FastQCFastQC Report
Mon 6 Jun 2016
ERR1211239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8064775
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293710.3641887095424237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245690.3046458208691501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156010.19344619037728888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172500.047.6717871
GTATCAA299050.039.620271
GTACTAG73350.037.7551231
TACCTGG53200.037.4550322
ACCTGGG58300.033.2153243
TAGGCAT91250.033.0586745
GGGCACC85200.032.94136494
CAACGCA356200.031.777975
ATCAACG356600.031.7383983
GTGGTAT55200.031.0895521
CTGTGCG102800.030.9439749
TATCAAC387000.030.3831162
CTAGGCA101800.030.24154
TAGTACT108050.029.6667144
AACGCAG403950.028.9288676
CTGGGGG45450.028.4303425
ACTGTGC116950.028.0035518
CCTGGGG56250.027.7412094
TCAACGC417550.027.5107574
GCATCGT92600.027.4515468
Produced by FastQC (version 0.11.5)