##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211239_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8064775 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16526896286629 33.0 31.0 34.0 30.0 34.0 2 32.304584442839385 34.0 31.0 34.0 31.0 34.0 3 32.381301400225055 34.0 31.0 34.0 30.0 34.0 4 35.99340328775446 37.0 35.0 37.0 35.0 37.0 5 35.88539233890592 37.0 35.0 37.0 35.0 37.0 6 35.915714821554225 37.0 35.0 37.0 35.0 37.0 7 35.856686640358845 37.0 35.0 37.0 35.0 37.0 8 35.849272546351266 37.0 35.0 37.0 35.0 37.0 9 37.44763691485503 39.0 37.0 39.0 34.0 39.0 10-11 37.42617090991379 39.0 37.0 39.0 34.0 39.0 12-13 37.31285534934328 39.0 37.0 39.0 34.0 39.0 14-15 38.51490934341008 40.0 38.0 41.0 34.0 41.0 16-17 38.47675031231498 40.0 38.0 41.0 34.0 41.0 18-19 38.50941142189336 40.0 38.0 41.0 34.0 41.0 20-21 38.47582821591427 40.0 38.0 41.0 34.0 41.0 22-23 38.337768009646894 40.0 38.0 41.0 34.0 41.0 24-25 38.26784361621992 40.0 38.0 41.0 34.0 41.0 26-27 38.121105176523834 40.0 37.0 41.0 33.0 41.0 28-29 38.1070502896857 40.0 37.0 41.0 33.5 41.0 30-31 38.108796525631526 40.0 37.0 41.0 33.5 41.0 32-33 37.96598689982052 40.0 37.0 41.0 33.0 41.0 34-35 37.822964682833685 40.0 37.0 41.0 33.0 41.0 36-37 37.68797585797496 40.0 36.5 41.0 33.0 41.0 38-39 37.58999302522389 40.0 36.0 41.0 33.0 41.0 40-41 37.47933965919694 40.0 36.0 41.0 32.5 41.0 42-43 37.31909830094454 40.0 36.0 41.0 32.0 41.0 44-45 37.14055513018032 39.0 36.0 41.0 31.0 41.0 46-47 36.86618355750781 39.0 35.0 41.0 31.0 41.0 48-49 36.58575484126959 39.0 35.0 41.0 30.0 41.0 50-51 36.35763365995952 39.0 35.0 40.5 30.0 41.0 52-53 36.13417305504493 38.5 35.0 40.0 29.5 41.0 54-55 35.87762560021823 38.0 35.0 40.0 29.0 41.0 56-57 35.59444634227241 38.0 34.5 40.0 28.5 41.0 58-59 35.29435340725563 37.0 34.0 40.0 28.0 41.0 60-61 35.02155590205554 37.0 34.0 40.0 28.0 41.0 62-63 34.698937912093015 36.0 34.0 39.5 27.0 41.0 64-65 34.32108769308505 36.0 33.0 39.0 26.0 41.0 66-67 34.018935804160684 35.0 33.0 39.0 26.0 40.0 68-69 33.70715506632237 35.0 33.0 38.0 26.0 40.0 70-71 33.4266869813479 35.0 33.0 37.5 26.0 40.0 72-73 33.11785232694031 35.0 32.0 37.0 25.0 39.5 74-75 32.80181641769299 35.0 32.0 36.5 25.0 39.0 76-77 31.83034356197166 34.0 30.5 35.0 24.0 38.0 78-79 32.345292274118 35.0 32.0 36.0 25.0 38.0 80-81 32.3011206760263 35.0 32.0 35.0 25.0 37.0 82-83 32.07457461615482 35.0 32.0 35.0 25.0 37.0 84-85 31.8432382180532 34.0 31.5 35.0 24.0 36.5 86-87 31.629675781407418 34.0 31.0 35.0 24.0 36.0 88-89 31.433965237715867 34.0 31.0 35.0 24.0 36.0 90-91 31.22843030338726 34.0 31.0 35.0 23.0 35.0 92-93 31.046990523604194 34.0 31.0 35.0 22.5 35.0 94-95 30.873780235654436 34.0 30.5 35.0 20.0 35.0 96-97 30.717433716377705 34.0 30.0 35.0 20.0 35.0 98-99 30.555765597924307 34.0 30.0 35.0 20.0 35.0 100 28.740215939068356 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3747624376630192 1101 2 -0.35372224737930225 1101 3 -0.41150056946225533 1101 4 -0.2223520551092193 1101 5 -0.2790981287276253 1101 6 -0.3210237096112678 1101 7 -0.3216786108105225 1101 8 -0.31905155287646636 1101 9 -0.45921959683444413 1101 10-11 -0.47263644449431297 1101 12-13 -0.45103242864620796 1101 14-15 -0.713856906089724 1101 16-17 -0.663899172936361 1101 18-19 -0.748406954920469 1101 20-21 -0.6708615445443513 1101 22-23 -0.7304632382712981 1101 24-25 -0.7048893857796372 1101 26-27 -0.801394955193409 1101 28-29 -0.8118391951369048 1101 30-31 -0.6544470033547611 1101 32-33 -0.6884409544498169 1101 34-35 -0.599053992100103 1101 36-37 -0.5874900685399425 1101 38-39 -0.6574765902671729 1101 40-41 -0.6593145277628167 1101 42-43 -0.7310641512508766 1101 44-45 -0.7307517459949509 1101 46-47 -0.6698074221898551 1101 48-49 -0.6773138554325371 1101 50-51 -0.723869758570018 1101 52-53 -0.7193146526828826 1101 54-55 -0.6514684302710734 1101 56-57 -0.6435653302178963 1101 58-59 -0.6631480819021149 1101 60-61 -0.7122076104439756 1101 62-63 -0.826072040822929 1101 64-65 -0.7078655710808945 1101 66-67 -0.7706642014014875 1101 68-69 -0.8804105204723314 1101 70-71 -0.7856275879602279 1101 72-73 -0.919782756063114 1101 74-75 -0.9474938714012779 1101 76-77 -0.8996412213299401 1101 78-79 -0.8292094921199933 1101 80-81 -0.6684023594308215 1101 82-83 -0.6187980053673741 1101 84-85 -0.6702232805666846 1101 86-87 -0.797825804914428 1101 88-89 -0.8479914866522726 1101 90-91 -0.8675785409757673 1101 92-93 -0.9204366582770867 1101 94-95 -0.9264316555305143 1101 96-97 -0.9372083757046425 1101 98-99 -1.080132760574596 1101 100 -0.9015908215029391 1102 1 -0.3836269223284141 1102 2 -0.3752048111221207 1102 3 -0.38878544394714254 1102 4 -0.24585594492047136 1102 5 -0.3836017577133646 1102 6 -0.40141113581520216 1102 7 -0.4016442198201844 1102 8 -0.46999276395238354 1102 9 -0.5626238892771696 1102 10-11 -0.5784235642999249 1102 12-13 -0.618698753277954 1102 14-15 -0.7393995700497342 1102 16-17 -0.7654391003781384 1102 18-19 -0.7742538764986975 1102 20-21 -0.8439025407258356 1102 22-23 -0.8224065961418106 1102 24-25 -0.8551407527847843 1102 26-27 -0.8942121412300637 1102 28-29 -0.874124318624176 1102 30-31 -0.8470053075303312 1102 32-33 -0.8186268499519258 1102 34-35 -0.8100053316612446 1102 36-37 -0.8848997272526233 1102 38-39 -0.9414804625228683 1102 40-41 -0.8280298241759638 1102 42-43 -0.8527227632578729 1102 44-45 -0.9745359300804424 1102 46-47 -0.9220735319271753 1102 48-49 -0.9168895410489384 1102 50-51 -0.9577458245330774 1102 52-53 -0.9990228845940266 1102 54-55 -0.9080339691301376 1102 56-57 -0.9456891771935716 1102 58-59 -1.0034210368760412 1102 60-61 -1.0285005526059123 1102 62-63 -0.9878193460439562 1102 64-65 -0.9964499943519698 1102 66-67 -1.0529461684701147 1102 68-69 -1.0721579994576018 1102 70-71 -1.0337253351873414 1102 72-73 -1.130466355369201 1102 74-75 -1.241897838528665 1102 76-77 -1.1087932858283658 1102 78-79 -0.9155552944414822 1102 80-81 -0.8951681176985957 1102 82-83 -0.8758567212029931 1102 84-85 -0.9352149972069945 1102 86-87 -0.9464995806288492 1102 88-89 -1.1126632083657846 1102 90-91 -1.084204474555552 1102 92-93 -1.104447705025759 1102 94-95 -1.2492077508006147 1102 96-97 -1.2974277288860314 1102 98-99 -1.2874815731377858 1102 100 -1.3120929715220342 1103 1 -0.3601425784246324 1103 2 -0.37789530752981904 1103 3 -0.4121590372454804 1103 4 -0.21485475181908242 1103 5 -0.281003695396592 1103 6 -0.33460772949344175 1103 7 -0.3707751362428553 1103 8 -0.31827986826577614 1103 9 -0.5686949312757434 1103 10-11 -0.5844321704894568 1103 12-13 -0.6381443012960872 1103 14-15 -0.6788875449696761 1103 16-17 -0.7362866955350924 1103 18-19 -0.6656153824940461 1103 20-21 -0.7187933933697934 1103 22-23 -0.7744080767193395 1103 24-25 -0.7871831665590108 1103 26-27 -0.8339860577860563 1103 28-29 -0.862369684346433 1103 30-31 -0.7603246954766831 1103 32-33 -0.7070067289635915 1103 34-35 -0.8726209441260195 1103 36-37 -0.8693921855053901 1103 38-39 -0.7874854976573076 1103 40-41 -0.8409549946060295 1103 42-43 -0.7538024264362448 1103 44-45 -0.7594483021897034 1103 46-47 -0.7886254210161994 1103 48-49 -0.9105918621194391 1103 50-51 -0.9974011228088671 1103 52-53 -0.9995220251370398 1103 54-55 -0.9754322142395111 1103 56-57 -0.9905640345975115 1103 58-59 -0.9874223641802686 1103 60-61 -0.9495047073945599 1103 62-63 -0.8663128752584441 1103 64-65 -0.9017175046072268 1103 66-67 -0.9273173288208625 1103 68-69 -1.0675949343676336 1103 70-71 -1.09248458957304 1103 72-73 -1.041492666487926 1103 74-75 -1.054727559073644 1103 76-77 -0.9688472029059128 1103 78-79 -0.8302485983761443 1103 80-81 -0.7587564124789914 1103 82-83 -0.7810368443875433 1103 84-85 -0.9357019060437075 1103 86-87 -0.9529040009315644 1103 88-89 -0.9221615762445943 1103 90-91 -1.0134484885811226 1103 92-93 -1.0245561423396836 1103 94-95 -1.0187663068348272 1103 96-97 -1.0666109161174404 1103 98-99 -1.0092752539668908 1103 100 -1.2553891050344355 1104 1 -0.4046664852426751 1104 2 -0.3309427757055907 1104 3 -0.42290325390067807 1104 4 -0.28247996248973095 1104 5 -0.3261138444635847 1104 6 -0.32054880597075197 1104 7 -0.304195408976625 1104 8 -0.334449263884693 1104 9 -0.48237384845488407 1104 10-11 -0.4603386651758399 1104 12-13 -0.5375818663340297 1104 14-15 -0.7549432930315803 1104 16-17 -0.6424893715088231 1104 18-19 -0.7937919675451681 1104 20-21 -0.7131704253737396 1104 22-23 -0.6988202117388411 1104 24-25 -0.7267135789322126 1104 26-27 -0.728338160949086 1104 28-29 -0.8461510378768438 1104 30-31 -0.7744080276992946 1104 32-33 -0.789455876783002 1104 34-35 -0.8057973465859831 1104 36-37 -0.6372067166627531 1104 38-39 -0.7298902037630199 1104 40-41 -0.772848030427383 1104 42-43 -0.687990774042575 1104 44-45 -0.7676087184412026 1104 46-47 -0.7680038351864837 1104 48-49 -0.8089131558141176 1104 50-51 -0.7511865843695276 1104 52-53 -0.6511670198039781 1104 54-55 -0.7597517674123893 1104 56-57 -0.7797434384877491 1104 58-59 -0.7750229234185255 1104 60-61 -0.7837901425688969 1104 62-63 -0.8868803880969907 1104 64-65 -0.8791206020685394 1104 66-67 -0.8990015301990866 1104 68-69 -0.9204951051147461 1104 70-71 -0.9130982540888155 1104 72-73 -0.9218496446724558 1104 74-75 -0.9110937110282862 1104 76-77 -0.7843940168418335 1104 78-79 -0.7205580681823385 1104 80-81 -0.6682292311566478 1104 82-83 -0.6625741633662834 1104 84-85 -0.6888404151483947 1104 86-87 -0.8144504050397252 1104 88-89 -0.7267075164520058 1104 90-91 -0.8058322590116909 1104 92-93 -0.7334572285349559 1104 94-95 -0.7703504341688117 1104 96-97 -0.8272949372884817 1104 98-99 -0.9150171175258883 1104 100 -1.0119523362456704 1105 1 -0.4437415090483867 1105 2 -0.4131585122867456 1105 3 -0.4942752502508867 1105 4 -0.26467661231940554 1105 5 -0.3520920259321585 1105 6 -0.4203370444810375 1105 7 -0.43617771375077297 1105 8 -0.4971273485668135 1105 9 -0.6351718574983423 1105 10-11 -0.5745955449243212 1105 12-13 -0.5851073320931377 1105 14-15 -0.8192450370094093 1105 16-17 -0.7578823202322909 1105 18-19 -0.7818045221841388 1105 20-21 -0.8597726308916975 1105 22-23 -0.8354412261976876 1105 24-25 -0.8104068387999135 1105 26-27 -0.9115151949144504 1105 28-29 -0.8880744566227463 1105 30-31 -0.7615167888382217 1105 32-33 -0.818111412408193 1105 34-35 -0.8231156736382204 1105 36-37 -0.8451572013604292 1105 38-39 -0.8707181669702777 1105 40-41 -0.9171639892897474 1105 42-43 -0.8715623608694187 1105 44-45 -0.8823599119631567 1105 46-47 -0.7951000952122556 1105 48-49 -0.8080726605854665 1105 50-51 -0.8681844438097954 1105 52-53 -0.8854293132228008 1105 54-55 -0.8309839766709572 1105 56-57 -0.8620473453883335 1105 58-59 -0.8761588251253585 1105 60-61 -0.837686152536051 1105 62-63 -0.8934668445904848 1105 64-65 -0.8903659222417915 1105 66-67 -0.933697937217211 1105 68-69 -1.0007364592508594 1105 70-71 -0.9877049257393438 1105 72-73 -1.0083897449773502 1105 74-75 -1.0729316004735594 1105 76-77 -1.0384280157678987 1105 78-79 -0.9202102158636869 1105 80-81 -0.8918365610073877 1105 82-83 -0.8196257926108785 1105 84-85 -0.8964657359987278 1105 86-87 -0.8923619228015518 1105 88-89 -0.9864742860940403 1105 90-91 -0.8971825461365768 1105 92-93 -0.9537405946146684 1105 94-95 -0.9413126198923933 1105 96-97 -0.9982213512616838 1105 98-99 -1.0812500596735397 1105 100 -1.1360959096339016 1106 1 -0.43008426480811934 1106 2 -0.4240032442999109 1106 3 -0.45075163811619845 1106 4 -0.3298597337327607 1106 5 -0.30340045107693925 1106 6 -0.4010412210423411 1106 7 -0.4803235893778819 1106 8 -0.41879746836495 1106 9 -0.5862328157809387 1106 10-11 -0.5286305855109745 1106 12-13 -0.6691204762010585 1106 14-15 -0.7589235269593004 1106 16-17 -0.7664132644762773 1106 18-19 -0.7176734751290965 1106 20-21 -0.7656376980254933 1106 22-23 -0.6885211768859847 1106 24-25 -0.7689927389605842 1106 26-27 -0.8426243738019821 1106 28-29 -0.823152259617494 1106 30-31 -0.8722786831616176 1106 32-33 -0.8874269989113799 1106 34-35 -0.8181563426424248 1106 36-37 -0.8715420588272522 1106 38-39 -0.9264924756367847 1106 40-41 -0.9254283451158045 1106 42-43 -0.8010256191535348 1106 44-45 -0.9156591557922198 1106 46-47 -0.8620483641936616 1106 48-49 -0.8706111665741361 1106 50-51 -0.9050074704891102 1106 52-53 -0.906708432551099 1106 54-55 -0.8721331846444613 1106 56-57 -0.9124266007694999 1106 58-59 -0.8258029945335821 1106 60-61 -0.8744888912800732 1106 62-63 -0.9333660382935065 1106 64-65 -0.9404402577315025 1106 66-67 -0.9004913852179044 1106 68-69 -0.883828053941599 1106 70-71 -0.9439563879109869 1106 72-73 -1.005880129149908 1106 74-75 -0.9733937147513991 1106 76-77 -0.9434948728206969 1106 78-79 -0.8111279964611171 1106 80-81 -0.6901867931186842 1106 82-83 -0.7198900376662323 1106 84-85 -0.7207073763355503 1106 86-87 -0.7538148086490075 1106 88-89 -0.8100426073243021 1106 90-91 -0.8351398309845273 1106 92-93 -0.8515771001528343 1106 94-95 -0.8164222198075919 1106 96-97 -0.8877905761886851 1106 98-99 -0.9268409788361218 1106 100 -0.9748760116369759 1107 1 -0.4052904288658574 1107 2 -0.41058638419018934 1107 3 -0.40723949575966145 1107 4 -0.25249728198213006 1107 5 -0.29686277193013666 1107 6 -0.327821830443348 1107 7 -0.3652833309771566 1107 8 -0.36300705409072975 1107 9 -0.5382026574944874 1107 10-11 -0.525576280869636 1107 12-13 -0.4913106114382231 1107 14-15 -0.6775589168479783 1107 16-17 -0.6852618491428544 1107 18-19 -0.6842589533339307 1107 20-21 -0.660774527935267 1107 22-23 -0.761108812026535 1107 24-25 -0.6794353508280864 1107 26-27 -0.6905948511218085 1107 28-29 -0.6786781189777784 1107 30-31 -0.6963842973524095 1107 32-33 -0.6622231504364606 1107 34-35 -0.7489917920339408 1107 36-37 -0.7889209184754904 1107 38-39 -0.7472085006676181 1107 40-41 -0.7265193306926676 1107 42-43 -0.6594801055333406 1107 44-45 -0.7684154593430392 1107 46-47 -0.7690377550243852 1107 48-49 -0.7208405840030139 1107 50-51 -0.6890007268333562 1107 52-53 -0.6920578146433343 1107 54-55 -0.7198270982512085 1107 56-57 -0.7355719351279575 1107 58-59 -0.7298519514169541 1107 60-61 -0.6578713087492218 1107 62-63 -0.6550287075121375 1107 64-65 -0.7072490734759427 1107 66-67 -0.6092024229074156 1107 68-69 -0.6691750780410644 1107 70-71 -0.7038167842213809 1107 72-73 -0.6955328175110367 1107 74-75 -0.6567971285345777 1107 76-77 -0.6890177740027177 1107 78-79 -0.5258825441724895 1107 80-81 -0.5823299730860185 1107 82-83 -0.6227686073831435 1107 84-85 -0.6060936771304064 1107 86-87 -0.5747983471113152 1107 88-89 -0.6253947077241726 1107 90-91 -0.6740014585742777 1107 92-93 -0.7596184441462874 1107 94-95 -0.7209300013703341 1107 96-97 -0.8306347399072109 1107 98-99 -0.8151788165011098 1107 100 -0.7956454053386786 1108 1 -0.41923489597512287 1108 2 -0.4232147954431298 1108 3 -0.4393431485621093 1108 4 -0.2888141626659504 1108 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1.2232848773673446 2310 78-79 1.1006705667258174 2310 80-81 1.0362623473075487 2310 82-83 1.0787835246938968 2310 84-85 1.1478242946522776 2310 86-87 1.141107789651695 2310 88-89 1.197650456054948 2310 90-91 1.2261614791355022 2310 92-93 1.2745266900149126 2310 94-95 1.3249432587521142 2310 96-97 1.2996366394454881 2310 98-99 1.2499682391163418 2310 100 1.5154522197904328 2311 1 0.430020399265608 2311 2 0.3838020180834647 2311 3 0.4653712044666136 2311 4 0.2966717413611235 2311 5 0.41477767406059485 2311 6 0.4587080090081983 2311 7 0.5066562936008054 2311 8 0.5187694924824626 2311 9 0.6943481487945036 2311 10-11 0.7011373104846541 2311 12-13 0.7583391215089463 2311 14-15 0.8574738808851521 2311 16-17 0.6607897932477016 2311 18-19 0.7530112693045297 2311 20-21 0.7724853014583033 2311 22-23 0.9137132954178355 2311 24-25 0.937717818149089 2311 26-27 0.31624877513315397 2311 28-29 0.6794833961254696 2311 30-31 0.9108143876599826 2311 32-33 1.0315554185511502 2311 34-35 1.068009872919589 2311 36-37 1.1251478203601337 2311 38-39 1.1231460274750944 2311 40-41 1.070114432006406 2311 42-43 1.1052572022631182 2311 44-45 1.0899757843263913 2311 46-47 0.8169649286090177 2311 48-49 1.063109270369317 2311 50-51 1.1600470414284914 2311 52-53 1.009711106469453 2311 54-55 1.0946841768792481 2311 56-57 1.2320950936281818 2311 58-59 1.2168504635686972 2311 60-61 1.216935315229584 2311 62-63 1.2015800973349613 2311 64-65 1.250644683349826 2311 66-67 1.2857628478995906 2311 68-69 1.2972337622956047 2311 70-71 1.2907868251186017 2311 72-73 1.3104306937630952 2311 74-75 1.3050685707905103 2311 76-77 1.2803245389599205 2311 78-79 1.013897449806997 2311 80-81 0.9891327660502895 2311 82-83 1.0754359519216834 2311 84-85 1.1323457116683606 2311 86-87 1.122556670992605 2311 88-89 1.2118966544594336 2311 90-91 1.2292202594106136 2311 92-93 1.3489807235330922 2311 94-95 1.3262309603656206 2311 96-97 1.3495551989686518 2311 98-99 1.449154207776342 2311 100 1.5803077493289024 2312 1 0.2648015770826788 2312 2 0.3720457348197783 2312 3 0.4780484247242711 2312 4 0.21276810706217475 2312 5 0.388014224594599 2312 6 0.4756537426993219 2312 7 0.36995489168921836 2312 8 0.4582568020534765 2312 9 0.6352560947213064 2312 10-11 0.6459249726730008 2312 12-13 0.7183190814436955 2312 14-15 0.9513679211796031 2312 16-17 0.9248650229308097 2312 18-19 0.8959145198060909 2312 20-21 0.9453384220305878 2312 22-23 1.0140978230066011 2312 24-25 0.9906926246318548 2312 26-27 1.045199009281184 2312 28-29 1.0058891680065685 2312 30-31 0.9957854642572315 2312 32-33 0.8864561000473117 2312 34-35 1.0028197564743806 2312 36-37 1.052136157793754 2312 38-39 0.878231249843175 2312 40-41 1.0063215808327186 2312 42-43 1.0653589011293292 2312 44-45 0.816308759349603 2312 46-47 0.8707706759702489 2312 48-49 1.0073113764365544 2312 50-51 0.814251873889674 2312 52-53 1.0017698213305124 2312 54-55 0.8530431184095804 2312 56-57 0.961538701829646 2312 58-59 1.0846563554818331 2312 60-61 1.0791570528215644 2312 62-63 1.1323534109333337 2312 64-65 1.0511153421046444 2312 66-67 1.0480513174949522 2312 68-69 1.1532003977822143 2312 70-71 1.057769673486824 2312 72-73 1.1015551280200668 2312 74-75 1.1040622700392149 2312 76-77 1.0740344430161564 2312 78-79 0.9863512201744911 2312 80-81 0.9799477904064489 2312 82-83 0.9650266709827378 2312 84-85 1.0225104059550496 2312 86-87 1.062075281538153 2312 88-89 1.041999894654463 2312 90-91 1.109218694826982 2312 92-93 1.148342453408194 2312 94-95 1.202833751649795 2312 96-97 0.7841788114936534 2312 98-99 0.5900116163495888 2312 100 0.886718337746295 2313 1 0.029379843688246865 2313 2 0.21307559242965368 2313 3 0.26426123283175684 2313 4 0.17492699512811782 2313 5 0.314468018662204 2313 6 0.2913462186638327 2313 7 0.25955928882408585 2313 8 0.3771968939423047 2313 9 0.5434687938870866 2313 10-11 0.37784665094598324 2313 12-13 0.397021514401807 2313 14-15 0.4442008929966761 2313 16-17 0.6992408526971019 2313 18-19 0.6862148179734291 2313 20-21 0.6239595749649567 2313 22-23 0.5200494478644941 2313 24-25 0.4424352577968236 2313 26-27 0.6462199776876929 2313 28-29 0.5202012329997174 2313 30-31 0.7813611731556662 2313 32-33 0.8818118597723412 2313 34-35 0.9366827810810747 2313 36-37 0.9748870163714898 2313 38-39 1.0320584060218252 2313 40-41 0.9535707781167702 2313 42-43 0.9305774402973483 2313 44-45 0.8148383043353746 2313 46-47 0.8753157391364681 2313 48-49 1.0181564266491208 2313 50-51 0.9782092456917013 2313 52-53 0.9772922232761374 2313 54-55 0.899953329984875 2313 56-57 0.6670469523465741 2313 58-59 0.7290147814967511 2313 60-61 0.6178405616454157 2313 62-63 0.9453617055339549 2313 64-65 1.0326754835565666 2313 66-67 0.9306214871922975 2313 68-69 1.0421521093717132 2313 70-71 1.029337621679744 2313 72-73 1.0329303736829374 2313 74-75 1.0891515640983158 2313 76-77 1.0323342686241794 2313 78-79 0.9238533430346152 2313 80-81 0.7982713622100945 2313 82-83 0.8644935678029668 2313 84-85 0.8840947600148716 2313 86-87 0.9131095087064658 2313 88-89 0.8966322659819888 2313 90-91 0.8528937214084422 2313 92-93 1.0155549667718375 2313 94-95 1.1291788451832936 2313 96-97 1.2242486921230693 2313 98-99 1.1915908773398591 2313 100 1.321404387092489 2314 1 -0.32209852489178914 2314 2 0.06624917267492236 2314 3 0.29557292768527077 2314 4 0.254302135433214 2314 5 0.362013184798478 2314 6 0.39689995417374746 2314 7 0.4295277957029313 2314 8 0.4460714102897896 2314 9 0.4439139844441087 2314 10-11 0.574803781306187 2314 12-13 0.4644684459442985 2314 14-15 0.7821940299350416 2314 16-17 0.8322978567681716 2314 18-19 0.8604319492941315 2314 20-21 0.9049040967457813 2314 22-23 0.9189809354102323 2314 24-25 0.8125476145247745 2314 26-27 0.7433167099038869 2314 28-29 0.8103190757763699 2314 30-31 0.8252197532157055 2314 32-33 0.8506888871459921 2314 34-35 0.896515091086151 2314 36-37 0.8546461877710669 2314 38-39 0.589779766483737 2314 40-41 0.6506129616656011 2314 42-43 0.888171851752233 2314 44-45 0.916128197728419 2314 46-47 0.8600517797798304 2314 48-49 0.877641027053734 2314 50-51 0.9167251009828945 2314 52-53 0.9267327936547929 2314 54-55 0.8192514192268092 2314 56-57 0.8028494401882966 2314 58-59 0.9377621349553209 2314 60-61 0.9585538195627663 2314 62-63 0.9137414245585873 2314 64-65 0.9480258487840629 2314 66-67 0.7077073292186427 2314 68-69 0.7133850721908885 2314 70-71 0.8728442287748166 2314 72-73 0.9932463554191813 2314 74-75 1.030443025592625 2314 76-77 0.8715113099352898 2314 78-79 0.8094164544329843 2314 80-81 0.8446235383848304 2314 82-83 0.8600381268133006 2314 84-85 0.8747489281173877 2314 86-87 0.9593421503734127 2314 88-89 0.9586979871896624 2314 90-91 1.0886591071091978 2314 92-93 0.9379858493765667 2314 94-95 0.9575101254480458 2314 96-97 0.8940769844862686 2314 98-99 1.065145673080341 2314 100 1.1710236520629778 2315 1 0.4187404995742554 2315 2 0.4363796608303758 2315 3 0.44303645652422574 2315 4 0.30565933138169754 2315 5 0.38772247215730005 2315 6 0.412391618833297 2315 7 0.3923775834670593 2315 8 0.28449335436518197 2315 9 0.5093285371689902 2315 10-11 0.551036439214073 2315 12-13 0.38403678097396465 2315 14-15 0.5885141007925583 2315 16-17 0.7231961557734223 2315 18-19 0.7355099029207821 2315 20-21 0.503423042584842 2315 22-23 0.8499799331493207 2315 24-25 0.8673012318579296 2315 26-27 0.806076048152697 2315 28-29 0.8371102286872016 2315 30-31 0.8297629151805523 2315 32-33 0.8128456063083433 2315 34-35 0.8462891939072463 2315 36-37 0.8136963189455244 2315 38-39 0.8971646424442312 2315 40-41 0.926563361404547 2315 42-43 1.000621544469908 2315 44-45 0.9786484630815337 2315 46-47 0.9583780105277455 2315 48-49 0.8857627072263128 2315 50-51 0.9338904377762276 2315 52-53 1.0269219153686322 2315 54-55 0.8351862023422072 2315 56-57 0.7876759674707685 2315 58-59 0.8169211940213756 2315 60-61 0.9296655910783542 2315 62-63 0.9782760206888739 2315 64-65 1.0331372888025 2315 66-67 1.0487069226166739 2315 68-69 1.103759450310008 2315 70-71 0.9449285753758048 2315 72-73 1.0694910236242094 2315 74-75 0.40743964846541303 2315 76-77 0.4309631224218542 2315 78-79 0.7219088792051451 2315 80-81 0.791923002032803 2315 82-83 0.7466213300081037 2315 84-85 0.8045719526663042 2315 86-87 0.8496641169802608 2315 88-89 0.8393673977721896 2315 90-91 0.9529832802439451 2315 92-93 0.9459851281112854 2315 94-95 1.0047807449592447 2315 96-97 1.1547128957279114 2315 98-99 1.1215685274779865 2315 100 1.0239162884235533 2316 1 0.33099044596398386 2316 2 0.1330144676802334 2316 3 0.26320795832655364 2316 4 0.04441598160788374 2316 5 0.29486080707341245 2316 6 0.3768531164489204 2316 7 0.4452222464371687 2316 8 0.4788451296309333 2316 9 0.6145231169155423 2316 10-11 0.6545220001817142 2316 12-13 0.4072020333506856 2316 14-15 0.5386473606032425 2316 16-17 0.8308127708721145 2316 18-19 0.7479320403877239 2316 20-21 0.7535823416688743 2316 22-23 0.7975100314858707 2316 24-25 0.49060472214170403 2316 26-27 0.7756347335268003 2316 28-29 0.9325606315300732 2316 30-31 0.5653247763248572 2316 32-33 0.7278592896912102 2316 34-35 0.8576216388418345 2316 36-37 0.9226050691625218 2316 38-39 0.799572126437738 2316 40-41 0.8733559356164733 2316 42-43 0.8275404091575922 2316 44-45 0.6494556294980143 2316 46-47 0.8049969895839908 2316 48-49 0.9393161745342624 2316 50-51 0.9533305423417744 2316 52-53 0.9039959945484526 2316 54-55 0.846037686580793 2316 56-57 0.9289577889945306 2316 58-59 0.8411590347018674 2316 60-61 0.9514439968621318 2316 62-63 0.9524476143945151 2316 64-65 0.9431898523944895 2316 66-67 0.9372187526159621 2316 68-69 0.9402074126092259 2316 70-71 0.9218322504873626 2316 72-73 0.9511769899875873 2316 74-75 0.9513149305575439 2316 76-77 0.8816771601430595 2316 78-79 0.6015660798300857 2316 80-81 0.6038295874160937 2316 82-83 0.5263547338006447 2316 84-85 0.6153144293586017 2316 86-87 0.6571124979501484 2316 88-89 0.6533368297075732 2316 90-91 0.5609512978893818 2316 92-93 0.2765048158442305 2316 94-95 0.39583331876271544 2316 96-97 0.6450400715389577 2316 98-99 0.8625368565735556 2316 100 0.5101524901615484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 6.0 12 39.0 13 188.0 14 739.0 15 2023.0 16 5094.0 17 10614.0 18 18330.0 19 26839.0 20 39134.0 21 56554.0 22 69308.0 23 69274.0 24 67205.0 25 69906.0 26 80894.0 27 99102.0 28 122705.0 29 150963.0 30 185891.0 31 232707.0 32 296846.0 33 394313.0 34 580216.0 35 802443.0 36 1256927.0 37 1812730.0 38 1387265.0 39 226489.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36425330245128 21.109687144943145 13.514684956065523 23.011374596540048 2 15.473054114962908 20.17071771996119 41.083551617993066 23.27267654708284 3 18.910806563109325 26.84559705633449 28.981763285398536 25.261833095157645 4 12.350628198498645 16.557318670965092 39.192161525522685 31.89989160501358 5 12.852175045181049 38.7454330715984 34.19970178957256 14.202690093647993 6 29.790254532829497 37.86368497571228 18.038680558354077 14.307379933104148 7 27.508804099441157 32.25171546797287 22.613711170483647 17.625769262102327 8 26.927694820996244 35.07810199292602 19.52771156045891 18.466491625618815 9 27.019389001531525 15.294456122075683 21.386446920515635 36.29970795587715 10-11 26.170982587335338 26.25503992165395 26.705556978417206 20.868420512593502 12-13 26.044266579042812 23.8901395265212 27.715782523380998 22.34981137105499 14-15 23.39306729438536 25.06492134482878 25.649600936618196 25.892410424167668 16-17 21.778962659787183 27.707819355353898 26.924273726756955 23.58894425810197 18-19 21.250078272487453 27.495553192742513 29.54655895545753 21.707809579312503 20-21 22.83434246479458 26.66905028392924 28.939399228013034 21.557208023263147 22-23 23.046774398541807 26.39536750870297 28.661109578382533 21.89674851437269 24-25 22.33422997538848 26.28916603981081 29.559583566421622 21.81702041837909 26-27 22.233333015075146 26.667082882243687 28.598374824901164 22.501209277780003 28-29 21.52039022619214 27.179536746202633 29.12630303808849 22.173769989516735 30-31 22.661800029239007 26.636693169839575 29.01802935779953 21.68347744312188 32-33 22.878461024276543 26.86143005340703 28.22878771061611 22.031321211700313 34-35 22.75067694881384 26.75575071614189 28.974159606124438 21.51941272891983 36-37 22.328193761566205 26.484741289064633 28.681812092857108 22.505252856512055 38-39 22.548144754492633 26.592321495189864 29.155977892033906 21.70355585828359 40-41 22.21443929874578 25.72407072854674 29.694614078490183 22.366875894217298 42-43 22.251726413687685 25.96223635709275 28.890963068681145 22.895074160538417 44-45 22.160099094418538 26.838474721269044 28.771360289401137 22.23006589491128 46-47 22.190598142199843 26.572935398658114 28.12511006659804 23.111356392543996 48-49 23.021608782865048 26.50442052927063 28.16753550804389 22.306435179820436 50-51 22.664666379582687 26.444446304599822 28.008750170901774 22.882137144915717 52-53 22.100674595323103 26.988190474017347 27.76124966773094 23.149885262928606 54-55 22.084767136408846 26.87245928689583 27.789611686712938 23.253161889982387 56-57 23.045360691286113 26.38618920693746 27.336325702416048 23.232124399360377 58-59 21.951078870477755 26.942378562122755 28.17356023909479 22.932982328304696 60-61 22.320202227059532 27.525431059630534 27.37377688521298 22.780589828096957 62-63 22.64515461844078 26.806148902266045 27.09197623854896 23.456720240744215 64-65 21.79310746891917 26.859399696781473 27.489944141181127 23.85754869311823 66-67 22.249389450778974 26.57065791668639 27.449518759976655 23.73043387255798 68-69 21.795502416215058 26.671903758602937 27.6594634629709 23.8731303622111 70-71 21.81584820417795 26.24414440914593 27.90977536142793 24.030232025248193 72-73 22.309592523434976 26.46411847223456 27.858072196250898 23.368216808079563 74-75 22.48725954640146 26.78015468094334 27.399797601546698 23.332788171108497 76-77 22.183045461353338 26.56653863053747 27.175034288193235 24.07538161991596 78-79 21.981859508293415 26.604630391635997 27.32909795587727 24.08441214419332 80-81 21.55356907815913 27.092774562012238 27.655523715126467 23.698132644702163 82-83 22.54368465365955 26.053968731441756 28.580963175791446 22.82138343910725 84-85 22.694632214479423 26.536252775971807 27.324426858473622 23.444688151075148 86-87 21.37539697615122 27.232796604165788 27.750148170103873 23.641658249579127 88-89 22.18763486504871 26.077611291351687 27.673563484817315 24.061190358782287 90-91 22.07799200137704 25.986952226914017 27.823205310911963 24.111850460796976 92-93 22.34443743927547 26.257576514070102 27.610102701014664 23.787883345639756 94-95 21.178162264790895 26.23311685915992 28.21943765906542 24.36928321698376 96-97 21.430547464525326 26.172622780918708 27.869866721822074 24.52696303273389 98-99 20.83684347181216 26.703632856124 27.67571461251761 24.783809059546233 100 21.341566953754352 26.231802644157092 27.095732458108053 25.330897943980506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1826.0 1 1305.5 2 1484.5 3 1982.5 4 1374.0 5 1130.5 6 1748.5 7 3255.0 8 4947.5 9 5643.0 10 5019.0 11 4248.5 12 4532.5 13 5326.0 14 6234.0 15 7379.5 16 8707.0 17 9951.5 18 10950.0 19 12003.0 20 13084.0 21 14000.5 22 15060.5 23 16446.0 24 17947.0 25 19941.0 26 23378.5 27 27169.5 28 32460.0 29 44783.0 30 63355.5 31 82018.0 32 108924.5 33 139700.0 34 164223.5 35 189469.0 36 213375.5 37 248119.5 38 263446.5 39 249966.5 40 231636.0 41 212068.0 42 207110.5 43 218333.5 44 241171.0 45 284375.5 46 321742.5 47 347134.5 48 368491.5 49 367597.5 50 357089.5 51 361341.5 52 361121.0 53 342670.0 54 323539.5 55 284370.0 56 247411.0 57 205191.0 58 163264.0 59 152411.0 60 125310.5 61 86140.5 62 63587.5 63 51877.5 64 36824.0 65 18889.5 66 10973.0 67 7048.0 68 5038.5 69 3770.5 70 2875.0 71 2187.0 72 1761.5 73 1668.5 74 1302.0 75 901.5 76 634.5 77 366.0 78 203.5 79 120.0 80 76.5 81 46.0 82 35.5 83 27.0 84 14.5 85 9.5 86 6.0 87 3.0 88 1.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04421698063492162 2 0.0 3 0.0 4 0.03216456751738269 5 0.0 6 0.0 7 8.183737302032605E-4 8 0.0 9 0.011469631824818423 10-11 5.14583481870232E-4 12-13 0.0 14-15 1.9839363156442678E-4 16-17 3.0379024833302853E-4 18-19 0.0 20-21 1.859940295916501E-5 22-23 0.0 24-25 6.881779094891053E-4 26-27 5.455824868021737E-4 28-29 2.0459343255081514E-4 30-31 0.002554318006391995 32-33 0.015251510426515309 34-35 0.026373953396095984 36-37 0.030937006922077803 38-39 0.022306883949025236 40-41 0.0013701560179918224 42-43 9.299701479582505E-5 44-45 0.01178582167512423 46-47 0.018289412909845596 48-49 0.003713680790846614 50-51 0.011562628839614248 52-53 0.011203040382403724 54-55 0.01051486247291462 56-57 4.8358447693829025E-4 58-59 0.008592924167134235 60-61 0.004643650938804865 62-63 0.0014383538288420942 64-65 0.036361832785167594 66-67 0.06228940051024362 68-69 0.06148342638201314 70-71 0.05351668211450413 72-73 0.046461308591994196 74-75 0.03663462402856869 76-77 0.03220796612428741 78-79 0.015710295699508044 80-81 9.981679588085223E-4 82-83 0.03921374123890623 84-85 0.053436084701681076 86-87 0.05929489663381805 88-89 0.07183089422829526 90-91 0.0497534029157664 92-93 0.040515699446047784 94-95 0.013298573115802983 96-97 0.003583484970132459 98-99 0.022585874993412712 100 0.03156938662268941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8064775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.9054335204024 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69928517710102 51.8671050734262 2 13.53590770522671 17.841797308102432 3 4.033136008817611 7.974167313236105 4 1.562834731777166 4.119972020740637 5 0.7354467964522813 2.423496997568937 6 0.4027394908161263 1.5925632442813757 7 0.23966833768082973 1.1056811987178494 8 0.1563649643138313 0.824424060840424 9 0.11968156600111483 0.7098898942533712 >10 0.46518127849956026 5.292961360364735 >50 0.02812386555008622 1.2880212599091934 >100 0.019542384997680764 2.451245449669758 >500 0.0014708973162696733 0.6676754578718708 >1k 5.21903842298547E-4 0.658110641654355 >5k 2.372290186950307E-5 0.09563734563881758 >10k+ 7.116870560850921E-5 1.0872513737238865 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29371 0.3641887095424237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24569 0.3046458208691501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15601 0.19344619037728888 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4799203945553348E-5 2 0.0 0.0 0.0 0.0 2.4799203945553348E-5 3 0.0 0.0 0.0 0.0 2.4799203945553348E-5 4 0.0 0.0 0.0 0.0 2.4799203945553348E-5 5 0.0 0.0 0.0 0.0 2.4799203945553348E-5 6 0.0 0.0 0.0 1.2399601972776674E-5 3.719880591833002E-5 7 0.0 0.0 0.0 1.2399601972776674E-5 3.719880591833002E-5 8 0.0 0.0 0.0 1.2399601972776674E-5 3.719880591833002E-5 9 0.0 0.0 0.0 2.4799203945553348E-5 4.9598407891106696E-5 10-11 0.0 0.0 0.0 2.4799203945553348E-5 5.579820887749503E-5 12-13 0.0 0.0 0.0 3.099900493194169E-5 6.199800986388336E-5 14-15 0.0 0.0 0.0 4.9598407891106696E-5 8.679721380943671E-5 16-17 0.0 0.0 0.0 8.059741282304838E-5 9.919681578221339E-5 18-19 0.0 0.0 0.0 1.1159641775499006E-4 1.1159641775499006E-4 20-21 0.0 0.0 0.0 1.9219383057803843E-4 1.363956217005434E-4 22-23 0.0 0.0 0.0 4.7738467595190195E-4 1.363956217005434E-4 24-25 0.0 0.0 0.0 0.001159362784454619 1.363956217005434E-4 26-27 0.0 0.0 0.0 0.002337324971868403 1.363956217005434E-4 28-29 0.0 0.0 0.0 0.006007607155810299 1.363956217005434E-4 30-31 0.0 0.0 0.0 0.020558540070863725 1.363956217005434E-4 32-33 0.0 0.0 0.0 0.053764674153959656 2.1079323353720346E-4 34-35 0.0 0.0 0.0 0.10293529577700555 2.1079323353720346E-4 36-37 0.0 0.0 0.0 0.18364430501780893 2.169930345235918E-4 38-39 0.0 0.0 0.0 0.3057121866388089 2.4179223846914512E-4 40-41 0.0 0.0 0.0 0.43676357988908554 2.6039164142831016E-4 42-43 0.0 0.0 0.0 0.5641570905573932 2.6039164142831016E-4 44-45 0.0 0.0 0.0 0.7096354207029953 2.727912434010868E-4 46-47 0.0 0.0 0.0 0.8782700075327582 2.727912434010868E-4 48-49 0.0 1.2399601972776674E-5 0.0 1.065280804486176 2.851908453738635E-4 50-51 0.0 1.2399601972776674E-5 0.0 1.2613619102826799 2.975904473466402E-4 52-53 0.0 1.2399601972776674E-5 0.0 1.446890954800351 2.975904473466402E-4 54-55 0.0 3.099900493194169E-5 0.0 1.6514285891422884 2.975904473466402E-4 56-57 0.0 3.719880591833002E-5 0.0 1.8821541828507304 2.975904473466402E-4 58-59 0.0 3.719880591833002E-5 0.0 2.1498119910350875 2.975904473466402E-4 60-61 0.0 3.719880591833002E-5 0.0 2.420309308071211 2.975904473466402E-4 62-63 0.0 3.719880591833002E-5 0.0 2.698004594052531 2.975904473466402E-4 64-65 0.0 3.719880591833002E-5 0.0 2.9751852965519805 2.975904473466402E-4 66-67 0.0 3.719880591833002E-5 0.0 3.2796017247846345 2.975904473466402E-4 68-69 0.0 3.719880591833002E-5 0.0 3.6153953458093993 2.975904473466402E-4 70-71 0.0 3.719880591833002E-5 0.0 3.977854310876621 2.975904473466402E-4 72-73 0.0 3.719880591833002E-5 0.0 4.342966790766017 3.037902483330285E-4 74-75 0.0 3.719880591833002E-5 0.0 4.688723491975908 3.0999004931941683E-4 76-77 0.0 3.719880591833002E-5 0.0 5.071015620398585 3.0999004931941683E-4 78-79 0.0 4.9598407891106696E-5 0.0 5.488138230762793 3.0999004931941683E-4 80-81 0.0 4.9598407891106696E-5 0.0 5.9252737987110615 3.0999004931941683E-4 82-83 0.0 4.9598407891106696E-5 0.0 6.370084720280479 3.0999004931941683E-4 84-85 0.0 4.9598407891106696E-5 0.0 6.801460673112393 3.0999004931941683E-4 86-87 0.0 4.9598407891106696E-5 0.0 7.2541949403424155 3.0999004931941683E-4 88 0.0 4.9598407891106696E-5 0.0 7.611532869794879 3.0999004931941683E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17250 0.0 47.671787 1 GTATCAA 29905 0.0 39.62027 1 GTACTAG 7335 0.0 37.755123 1 TACCTGG 5320 0.0 37.455032 2 ACCTGGG 5830 0.0 33.215324 3 TAGGCAT 9125 0.0 33.058674 5 GGGCACC 8520 0.0 32.941364 94 CAACGCA 35620 0.0 31.77797 5 ATCAACG 35660 0.0 31.738398 3 GTGGTAT 5520 0.0 31.089552 1 CTGTGCG 10280 0.0 30.943974 9 TATCAAC 38700 0.0 30.383116 2 CTAGGCA 10180 0.0 30.2415 4 TAGTACT 10805 0.0 29.666714 4 AACGCAG 40395 0.0 28.928867 6 CTGGGGG 4545 0.0 28.430342 5 ACTGTGC 11695 0.0 28.003551 8 CCTGGGG 5625 0.0 27.741209 4 TCAACGC 41755 0.0 27.510757 4 GCATCGT 9260 0.0 27.451546 8 >>END_MODULE