FastQCFastQC Report
Mon 6 Jun 2016
ERR1211238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9718232
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383250.39436185511932625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304330.31315366828040325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181810.18708135389235409No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205350.048.1776921
GTATCAA363800.040.6232761
TACCTGG52900.036.477872
GTACTAG90850.036.0572281
GGGCACC96650.034.76741894
CAACGCA415750.034.3271985
ATCAACG421700.033.8653263
GCATCGT90850.033.167028
TATCAAC457450.032.1622662
GTGGTAT61250.031.8896681
TAGGCAT113200.031.1861955
AACGCAG471900.030.9104396
CTGTGCG122700.030.5334539
CTAACGC49900.029.580143
ACCTGGG64350.029.5123583
TAACGCC49550.029.5040154
TAGTACT129000.029.333954
TCTAACG50750.029.2129782
TCAACGC494500.029.1073914
CGCCTAC51000.028.6657667
Produced by FastQC (version 0.11.5)