##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9718232 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47292748310598 31.0 31.0 34.0 30.0 34.0 2 31.48879847692461 31.0 31.0 34.0 28.0 34.0 3 31.549475974642302 33.0 31.0 34.0 28.0 34.0 4 35.15087806094771 37.0 35.0 37.0 32.0 37.0 5 35.21783386113853 37.0 35.0 37.0 32.0 37.0 6 35.28014396034176 37.0 35.0 37.0 32.0 37.0 7 35.19846552335857 37.0 35.0 37.0 32.0 37.0 8 35.207393793438975 37.0 35.0 37.0 32.0 37.0 9 36.76806768967853 39.0 37.0 39.0 32.0 39.0 10-11 36.721206593956595 39.0 37.0 39.0 32.0 39.0 12-13 36.60808133619366 39.0 37.0 39.0 32.0 39.0 14-15 37.78358409225052 40.0 37.0 41.0 32.0 41.0 16-17 37.77020799668088 40.0 37.0 41.0 32.0 41.0 18-19 37.803516524404856 40.0 37.0 41.0 32.0 41.0 20-21 37.78108250554216 40.0 37.0 41.0 32.0 41.0 22-23 37.665456021218674 40.0 37.0 41.0 32.0 41.0 24-25 37.567814701274884 40.0 37.0 41.0 32.0 41.0 26-27 37.41271812609536 40.0 37.0 41.0 31.0 41.0 28-29 37.30296421200893 40.0 36.0 41.0 31.0 41.0 30-31 37.1492874938569 39.5 36.0 41.0 31.0 41.0 32-33 36.9607555160239 39.0 36.0 41.0 30.0 41.0 34-35 36.813345729964055 39.0 35.0 41.0 30.0 41.0 36-37 36.70454023941804 39.0 35.0 41.0 30.0 41.0 38-39 36.55532822225277 39.0 35.0 41.0 30.0 41.0 40-41 36.38680451341355 39.0 35.0 40.5 29.5 41.0 42-43 36.165731019798656 39.0 35.0 40.0 28.5 41.0 44-45 35.908365894125595 38.0 35.0 40.0 27.5 41.0 46-47 35.635253356783416 38.0 34.0 40.0 27.0 41.0 48-49 35.38734154525227 38.0 34.0 40.0 26.0 41.0 50-51 34.67903981917699 37.5 33.5 39.5 25.0 40.5 52-53 34.76454791365343 38.0 33.5 39.5 25.5 40.5 54-55 35.219680596223675 38.0 34.0 40.0 26.0 41.0 56-57 35.15128137504846 38.0 34.0 40.0 26.0 41.0 58-59 34.914513308593584 37.5 34.0 40.0 26.0 41.0 60-61 34.69395719303676 37.0 34.0 40.0 26.0 41.0 62-63 34.395533158706236 36.0 33.0 40.0 25.5 41.0 64-65 34.15183173235626 36.0 33.0 39.5 25.0 41.0 66-67 33.992675519580104 35.5 33.0 39.0 25.0 41.0 68-69 33.71571588330059 35.0 33.0 39.0 25.0 41.0 70-71 33.45567887245334 35.0 33.0 38.5 24.0 41.0 72-73 33.155103623786715 35.0 32.5 37.5 24.0 40.0 74-75 32.84285757944449 35.0 32.0 37.0 23.5 39.5 76-77 32.5404819518612 35.0 32.0 36.5 23.0 39.0 78-79 32.22736676794709 35.0 31.5 36.0 22.0 39.0 80-81 31.95483869905555 35.0 31.0 36.0 21.5 38.5 82-83 31.67305843285075 35.0 31.0 35.0 20.5 37.0 84-85 31.43781260830159 35.0 31.0 35.0 20.0 37.0 86-87 31.22136994671459 34.5 31.0 35.0 20.0 36.5 88-89 31.024727337235824 34.0 31.0 35.0 20.0 36.0 90-91 30.798532284473144 34.0 30.0 35.0 19.0 36.0 92-93 30.59689838645548 34.0 30.0 35.0 18.5 36.0 94-95 30.44520376751656 34.0 30.0 35.0 18.0 35.0 96-97 30.265157952598784 34.0 29.0 35.0 18.0 35.0 98-99 30.040817506723446 34.0 29.0 35.0 17.0 35.0 100 28.885222538420567 33.0 27.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.46748698241810516 1101 2 -0.3530554884944479 1101 3 -0.43645755417844256 1101 4 -0.35464224086019414 1101 5 -0.3352288515555699 1101 6 -0.2850501720686367 1101 7 -0.3259600099778268 1101 8 -0.3284416506512855 1101 9 -0.5343096823858602 1101 10-11 -0.4998793919879603 1101 12-13 -0.5030155061005672 1101 14-15 -0.6558971521770474 1101 16-17 -0.674177817860695 1101 18-19 -0.6829220248329051 1101 20-21 -0.6221898167561264 1101 22-23 -0.7524741212308115 1101 24-25 -0.8253431870510539 1101 26-27 -0.8263734428896825 1101 28-29 -0.8047793378207899 1101 30-31 -0.8280136831837694 1101 32-33 -0.9368864903526273 1101 34-35 -0.8626907808905884 1101 36-37 -0.9505516526692261 1101 38-39 -0.9784082472289484 1101 40-41 -0.9183543955928499 1101 42-43 -1.0443652767415514 1101 44-45 -1.0167418749461277 1101 46-47 -1.113964324939694 1101 48-49 -1.1159967022138204 1101 50-51 -1.1248277415697387 1101 52-53 -1.110371022317608 1101 54-55 -0.9280138764689312 1101 56-57 -0.7559383169515499 1101 58-59 -0.7882993654365364 1101 60-61 -0.7943630188118718 1101 62-63 -0.8595322307038984 1101 64-65 -0.7893759228936759 1101 66-67 -0.8199218116369664 1101 68-69 -0.8016188711321703 1101 70-71 -0.9543727940597293 1101 72-73 -0.9265575992716606 1101 74-75 -1.0106667388113628 1101 76-77 -0.9129753541250878 1101 78-79 -0.9610249815396941 1101 80-81 -1.0831187669199274 1101 82-83 -1.0351510133817499 1101 84-85 -1.1358449655980323 1101 86-87 -1.1464468419330949 1101 88-89 -1.1313623182689483 1101 90-91 -1.1418619852017287 1101 92-93 -1.307899610706876 1101 94-95 -1.3904548828329624 1101 96-97 -1.5193837736198965 1101 98-99 -1.5362659457096974 1101 100 -1.5604078868941365 1102 1 -0.4517875953398658 1102 2 -0.4594412199036988 1102 3 -0.4592849232841303 1102 4 -0.30200641254462113 1102 5 -0.32836357524269744 1102 6 -0.35805664242021606 1102 7 -0.3552188792976452 1102 8 -0.46017902394238064 1102 9 -0.5720420732261005 1102 10-11 -0.6080719932545477 1102 12-13 -0.5979298237352424 1102 14-15 -0.7127560677631521 1102 16-17 -0.7314836055949456 1102 18-19 -0.734079457972868 1102 20-21 -0.8290668284322038 1102 22-23 -0.750516672716131 1102 24-25 -0.7828199222783354 1102 26-27 -0.8306980634092653 1102 28-29 -0.8390047635763977 1102 30-31 -0.8796531679104191 1102 32-33 -0.8732723008267769 1102 34-35 -0.9091026395476547 1102 36-37 -0.885904407678261 1102 38-39 -1.0180103526768889 1102 40-41 -1.0904925334622462 1102 42-43 -1.039544042775951 1102 44-45 -1.1412556110066419 1102 46-47 -1.074630211005811 1102 48-49 -1.1366164282962856 1102 50-51 -1.0900704488987785 1102 52-53 -1.0136938165268319 1102 54-55 -0.9242944725862969 1102 56-57 -0.9375419076943317 1102 58-59 -0.8864967123738481 1102 60-61 -0.8169764833096309 1102 62-63 -0.9307972966389855 1102 64-65 -0.8546211739211742 1102 66-67 -0.9684951373429627 1102 68-69 -0.9420791905318637 1102 70-71 -0.9510077608344076 1102 72-73 -0.9436050132779172 1102 74-75 -0.9241820942513215 1102 76-77 -1.048898703044177 1102 78-79 -0.9820939043375887 1102 80-81 -1.162938255239137 1102 82-83 -1.1077469310981094 1102 84-85 -1.0740601142788364 1102 86-87 -1.1731496570521749 1102 88-89 -1.2313285942760466 1102 90-91 -1.238106614192798 1102 92-93 -1.2631822505482546 1102 94-95 -1.3212849627098358 1102 96-97 -1.386043751850103 1102 98-99 -1.435685310261249 1102 100 -1.5143605485177432 1103 1 -0.5579473603707328 1103 2 -0.441580179550229 1103 3 -0.5511517458712554 1103 4 -0.4639364792373897 1103 5 -0.38658243983407203 1103 6 -0.5122231729354283 1103 7 -0.5039464201427961 1103 8 -0.4817098915358855 1103 9 -0.6343681954850595 1103 10-11 -0.6591763229163234 1103 12-13 -0.7197964379679433 1103 14-15 -0.8178709012202319 1103 16-17 -0.8601677785491546 1103 18-19 -0.8292937846505097 1103 20-21 -0.8236204666590226 1103 22-23 -0.8581051741024766 1103 24-25 -0.8246741126671466 1103 26-27 -0.8973832476224644 1103 28-29 -0.9972063250732219 1103 30-31 -1.0297107756305053 1103 32-33 -1.0578121665987155 1103 34-35 -1.0876638720084557 1103 36-37 -0.9983457880729745 1103 38-39 -1.0047743677280394 1103 40-41 -1.0478387260584654 1103 42-43 -1.13835104578434 1103 44-45 -1.181779933766542 1103 46-47 -1.1973185585628414 1103 48-49 -1.190923438648312 1103 50-51 -1.2983228693680289 1103 52-53 -1.1928275966874153 1103 54-55 -0.8815067718501908 1103 56-57 -0.8707363931107182 1103 58-59 -0.9076955222260779 1103 60-61 -0.9725387607304654 1103 62-63 -0.930837587032677 1103 64-65 -0.8190730776942203 1103 66-67 -0.8728665212409865 1103 68-69 -0.8440221922769169 1103 70-71 -0.8604597904170959 1103 72-73 -0.8777790775701675 1103 74-75 -0.8484144220677408 1103 76-77 -0.899538627212273 1103 78-79 -0.9411706340976913 1103 80-81 -1.0220454810273587 1103 82-83 -0.9946850131740383 1103 84-85 -1.0304141043399895 1103 86-87 -1.006117625349514 1103 88-89 -1.0397256844414748 1103 90-91 -1.336898332184294 1103 92-93 -1.3073017544461365 1103 94-95 -1.3465566318257096 1103 96-97 -1.3148885825712462 1103 98-99 -1.2988875685499153 1103 100 -1.4365766630584815 1104 1 -0.5328073823000388 1104 2 -0.47235911831776534 1104 3 -0.5703065460749634 1104 4 -0.4059110195780846 1104 5 -0.3967146420472929 1104 6 -0.3871756073077748 1104 7 -0.4138385271821008 1104 8 -0.37952492844012653 1104 9 -0.5460995294392603 1104 10-11 -0.5329634112999031 1104 12-13 -0.5700986948919251 1104 14-15 -0.7398059947333735 1104 16-17 -0.8597125167615047 1104 18-19 -0.7977258400369962 1104 20-21 -0.7946660941444534 1104 22-23 -0.7059301528105522 1104 24-25 -0.8077044983903789 1104 26-27 -0.892559984959135 1104 28-29 -0.8374050964744271 1104 30-31 -0.8441628091376927 1104 32-33 -0.8382441273091175 1104 34-35 -1.0224698921572326 1104 36-37 -0.977790201704515 1104 38-39 -0.9342979978481551 1104 40-41 -0.9512539388036956 1104 42-43 -0.94741282673764 1104 44-45 -0.9694198924753792 1104 46-47 -0.9985010082042862 1104 48-49 -0.9604423912156221 1104 50-51 -1.023846106218258 1104 52-53 -0.7934993050094334 1104 54-55 -0.727455369259637 1104 56-57 -0.7653444000278355 1104 58-59 -0.8007883925328656 1104 60-61 -0.7381592340472878 1104 62-63 -0.736441211322223 1104 64-65 -0.6601799609307051 1104 66-67 -0.7366701947871022 1104 68-69 -0.7952564352243812 1104 70-71 -0.8019003103724742 1104 72-73 -0.7860577848786008 1104 74-75 -0.8116054063068603 1104 76-77 -0.7639556572002171 1104 78-79 -0.9058376847586622 1104 80-81 -0.8973862055444961 1104 82-83 -1.0062860058272136 1104 84-85 -0.9324563712321385 1104 86-87 -0.8700638157472973 1104 88-89 -0.947829129582896 1104 90-91 -0.9360491496880705 1104 92-93 -1.0596854299743548 1104 94-95 -1.0532398922601764 1104 96-97 -1.1602901973419328 1104 98-99 -1.1599446438519365 1104 100 -1.1544121966279057 1105 1 -0.5702997355125596 1105 2 -0.4909344323402358 1105 3 -0.5787624516163739 1105 4 -0.3827466583940975 1105 5 -0.28351820645011827 1105 6 -0.34758541218780437 1105 7 -0.26905480683301874 1105 8 -0.40794997326833027 1105 9 -0.5615599897768178 1105 10-11 -0.5528361492889431 1105 12-13 -0.5702639187487932 1105 14-15 -0.7315173605216074 1105 16-17 -0.7580823529583114 1105 18-19 -0.7347982232232084 1105 20-21 -0.7336576788743869 1105 22-23 -0.7490976557204831 1105 24-25 -0.7791223738062598 1105 26-27 -0.7634969109839957 1105 28-29 -0.7991058100402739 1105 30-31 -0.7663874823672501 1105 32-33 -0.8349484615002112 1105 34-35 -0.8410349701241415 1105 36-37 -0.8770435714620177 1105 38-39 -0.8577707659958875 1105 40-41 -0.9038614725641381 1105 42-43 -0.8814441568388744 1105 44-45 -0.9059621241262334 1105 46-47 -1.0110235151958804 1105 48-49 -0.9380232381636588 1105 50-51 -0.9165512524906987 1105 52-53 -0.8070658268698381 1105 54-55 -0.6906028218509306 1105 56-57 -0.6569288198878027 1105 58-59 -0.6749911815361571 1105 60-61 -0.6441708927628014 1105 62-63 -0.7080100044513742 1105 64-65 -0.6821824892521278 1105 66-67 -0.7045272664826498 1105 68-69 -0.7448800358110859 1105 70-71 -0.7610515867271062 1105 72-73 -0.6844464604414711 1105 74-75 -0.6188009857333441 1105 76-77 -0.6761701771889363 1105 78-79 -0.7515627048745941 1105 80-81 -0.7475414857484743 1105 82-83 -0.8356346322601524 1105 84-85 -0.8093120177165645 1105 86-87 -0.8413049936090609 1105 88-89 -0.8890980600549483 1105 90-91 -1.0068864547151897 1105 92-93 -0.9538288440989575 1105 94-95 -0.9704781123676369 1105 96-97 -1.025251112918376 1105 98-99 -1.1032812470799591 1105 100 -1.0567096089114578 1106 1 -0.5655446953975805 1106 2 -0.5684667059382207 1106 3 -0.6453315104757849 1106 4 -0.39347450486461355 1106 5 -0.4259837325752116 1106 6 -0.511831701192861 1106 7 -0.5302142651090662 1106 8 -0.4953531499697874 1106 9 -0.6674772757696701 1106 10-11 -0.6022599666416681 1106 12-13 -0.6839521082020354 1106 14-15 -0.8679682099105293 1106 16-17 -0.8429589993856439 1106 18-19 -0.9394398933498564 1106 20-21 -0.8420476528498781 1106 22-23 -0.8783781820738099 1106 24-25 -0.8445807499079621 1106 26-27 -0.909197695738996 1106 28-29 -0.9623789179414359 1106 30-31 -1.0002512719516545 1106 32-33 -0.9965668221536461 1106 34-35 -1.0409651298032685 1106 36-37 -0.9484506660840069 1106 38-39 -1.0414552179838594 1106 40-41 -1.126859137603219 1106 42-43 -1.0715367556871271 1106 44-45 -1.0434259775023875 1106 46-47 -1.0408839466342457 1106 48-49 -0.9641360482522714 1106 50-51 -0.998385023635727 1106 52-53 -0.9964835655975364 1106 54-55 -0.936210298334295 1106 56-57 -0.8334127748661544 1106 58-59 -0.7796504150571124 1106 60-61 -0.8371396617917668 1106 62-63 -0.8587390982046443 1106 64-65 -0.8545792200939601 1106 66-67 -0.9550082436360228 1106 68-69 -0.9782771337622975 1106 70-71 -0.9194916742948678 1106 72-73 -0.8344285307517794 1106 74-75 -0.7745040953683144 1106 76-77 -0.7855327436911139 1106 78-79 -0.818320578049601 1106 80-81 -0.7716118577925108 1106 82-83 -0.9079039799669602 1106 84-85 -0.8892063580896306 1106 86-87 -0.8708338870206696 1106 88-89 -0.8330048364382527 1106 90-91 -0.9758711909671653 1106 92-93 -1.0031021860534821 1106 94-95 -1.0687517465444856 1106 96-97 -1.0101982633813122 1106 98-99 -1.016971026943942 1106 100 -1.1611573428485116 1107 1 -0.591574284117506 1107 2 -0.5751155265284282 1107 3 -0.5551592114428203 1107 4 -0.4938952299084889 1107 5 -0.4196074777637264 1107 6 -0.48364740702034936 1107 7 -0.4998093250094229 1107 8 -0.4752324422677816 1107 9 -0.7021396897878134 1107 10-11 -0.677478017787486 1107 12-13 -0.6933264388633802 1107 14-15 -0.8146330606537333 1107 16-17 -0.8298305522454186 1107 18-19 -0.8783374761528648 1107 20-21 -0.874963914651282 1107 22-23 -0.9283780491293072 1107 24-25 -0.9114812610352772 1107 26-27 -0.9637500848847012 1107 28-29 -1.024696329742369 1107 30-31 -0.9709398151782551 1107 32-33 -1.0563255249225278 1107 34-35 -1.1141473278840834 1107 36-37 -1.0919151880702245 1107 38-39 -1.1227607093895884 1107 40-41 -1.157619689482452 1107 42-43 -1.0726586005078076 1107 44-45 -1.1261251409269306 1107 46-47 -1.0227105509568304 1107 48-49 -1.0853392196582092 1107 50-51 -1.0334462416260095 1107 52-53 -1.0058501263911452 1107 54-55 -0.8945170698478933 1107 56-57 -0.9520873417846616 1107 58-59 -0.980244056587317 1107 60-61 -0.9187757714101252 1107 62-63 -0.831151222625337 1107 64-65 -0.8108809225812834 1107 66-67 -0.88106586887492 1107 68-69 -0.8630139523884708 1107 70-71 -0.9167569467380474 1107 72-73 -0.8099431663169128 1107 74-75 -0.8399432804953086 1107 76-77 -0.7985153063943926 1107 78-79 -0.7850025579537103 1107 80-81 -0.819739157178514 1107 82-83 -0.9194116563304036 1107 84-85 -0.8738383278161024 1107 86-87 -0.8585817805149887 1107 88-89 -0.8365533911689909 1107 90-91 -0.9105559987486167 1107 92-93 -0.9721849369175501 1107 94-95 -0.982880763573494 1107 96-97 -0.9722052645343844 1107 98-99 -0.9527387120344528 1107 100 -1.029020903970931 1108 1 -0.6248198896624686 1108 2 -0.5852413605701727 1108 3 -0.6541426204160885 1108 4 -0.42197837388723514 1108 5 -0.48087622830952625 1108 6 -0.40852677169797147 1108 7 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1.0974602807021725 2311 68-69 1.0955195912865676 2311 70-71 1.0435493471980664 2311 72-73 1.0972011007497997 2311 74-75 1.0882029164280596 2311 76-77 1.0908637212938501 2311 78-79 1.1457010918770223 2311 80-81 1.06950430234755 2311 82-83 1.1112364916920114 2311 84-85 1.1490035810736359 2311 86-87 1.164282209791498 2311 88-89 1.2425264136803627 2311 90-91 1.2427519846850323 2311 92-93 1.2460207425799723 2311 94-95 1.296533085146148 2311 96-97 1.325716773757403 2311 98-99 1.483439800288167 2311 100 1.5471810031107793 2312 1 0.628053849893611 2312 2 0.6366865919754296 2312 3 0.6791520663630983 2312 4 0.41655849937816214 2312 5 0.520676487585952 2312 6 0.5786560490412427 2312 7 0.4192305311216984 2312 8 0.6025640621040793 2312 9 0.7806786952940712 2312 10-11 0.7394357319784888 2312 12-13 0.8338821179700773 2312 14-15 1.0430239316261378 2312 16-17 1.0321450048966057 2312 18-19 0.9989512652847736 2312 20-21 1.0022027027588152 2312 22-23 1.0849834910329719 2312 24-25 1.0603492696366175 2312 26-27 1.1013805368311935 2312 28-29 1.1065666117382307 2312 30-31 1.104349605925563 2312 32-33 1.1000382312269394 2312 34-35 1.1169220380796858 2312 36-37 1.1503277447600766 2312 38-39 1.191470615516529 2312 40-41 1.1723420364899866 2312 42-43 1.2222273953538974 2312 44-45 1.270219396786537 2312 46-47 1.3279110123274975 2312 48-49 1.335444216586609 2312 50-51 1.3332003874195593 2312 52-53 1.2054107669716174 2312 54-55 1.1078335292149362 2312 56-57 1.0502038795412219 2312 58-59 1.0252400045707262 2312 60-61 1.005358235795228 2312 62-63 1.0090313464681628 2312 64-65 0.9957726082770861 2312 66-67 0.9668703150860765 2312 68-69 1.0037311603240795 2312 70-71 0.9893983033854425 2312 72-73 1.027689882246463 2312 74-75 1.0126646500104926 2312 76-77 1.0242705954083604 2312 78-79 1.04767691151212 2312 80-81 1.0266063855178125 2312 82-83 1.0811409725744738 2312 84-85 1.090645582780624 2312 86-87 1.0514300773521477 2312 88-89 0.9869525250161821 2312 90-91 1.049043481154147 2312 92-93 1.1708648622161562 2312 94-95 1.2525612688293855 2312 96-97 1.2453291040181362 2312 98-99 0.9293075391375609 2312 100 1.2647643937254642 2313 1 0.6742254054562622 2313 2 0.6511669084408815 2313 3 0.6531351475967888 2313 4 0.5500678646219299 2313 5 0.44564008453300374 2313 6 0.5429462294471392 2313 7 0.6152612596768066 2313 8 0.6186924931398892 2313 9 0.7498958092552996 2313 10-11 0.7776479027571739 2313 12-13 0.8697381525489831 2313 14-15 1.011592232539897 2313 16-17 1.0187567393415833 2313 18-19 1.009730928891571 2313 20-21 1.0114952402660862 2313 22-23 1.0245744773648937 2313 24-25 1.0351163292964998 2313 26-27 1.076266327984463 2313 28-29 1.1020410732437398 2313 30-31 1.122892749386132 2313 32-33 1.1336915150012388 2313 34-35 1.1798924155206194 2313 36-37 1.2449297422389307 2313 38-39 1.2619569438847904 2313 40-41 1.2139016795122757 2313 42-43 1.370753552448825 2313 44-45 1.3665479654764852 2313 46-47 1.3695218614727125 2313 48-49 1.3124901909355486 2313 50-51 1.3365961221828613 2313 52-53 1.2672028747719892 2313 54-55 1.2176401507542494 2313 56-57 1.1352353243238085 2313 58-59 1.1332008833442728 2313 60-61 1.0901545806092088 2313 62-63 1.0710759385069437 2313 64-65 1.0980326927806843 2313 66-67 1.113528578431179 2313 68-69 1.1064574953073034 2313 70-71 1.1017299226245925 2313 72-73 1.1154082635842002 2313 74-75 1.0800677647864703 2313 76-77 0.8944492321857709 2313 78-79 1.060992467109088 2313 80-81 1.1493823712476932 2313 82-83 1.1497843205784903 2313 84-85 1.1758881053627945 2313 86-87 1.2348914019558528 2313 88-89 1.3119104048645163 2313 90-91 1.3188885699666493 2313 92-93 1.3394175797831949 2313 94-95 1.4065011042420288 2313 96-97 1.447222939195882 2313 98-99 1.4892211140593616 2313 100 1.645396431192946 2314 1 0.6034295155722447 2314 2 0.6137893885962136 2314 3 0.6531913298894843 2314 4 0.5257410998765479 2314 5 0.5156148306216579 2314 6 0.544577641396458 2314 7 0.6183461802859043 2314 8 0.5933092281226067 2314 9 0.7324963971607801 2314 10-11 0.7389049238547116 2314 12-13 0.8099322517064209 2314 14-15 0.9731129203800322 2314 16-17 0.9881569754722861 2314 18-19 0.9547570886803101 2314 20-21 1.0135392290584022 2314 22-23 1.0472898211821544 2314 24-25 1.0173428835153189 2314 26-27 1.0227773488109548 2314 28-29 1.0492761240117687 2314 30-31 1.0662045494607213 2314 32-33 1.0344243499029773 2314 34-35 1.0795207702387586 2314 36-37 1.072882729959865 2314 38-39 1.0955634902759144 2314 40-41 1.1896427396199911 2314 42-43 1.2033508444287193 2314 44-45 1.2925637085700217 2314 46-47 1.2736075200404642 2314 48-49 1.2279508172607407 2314 50-51 1.1975190587682008 2314 52-53 1.1601010005790542 2314 54-55 1.0778739741232428 2314 56-57 1.0136971171327644 2314 58-59 0.9721468072667108 2314 60-61 0.9776410193818776 2314 62-63 1.0605467435998506 2314 64-65 1.0599671151462644 2314 66-67 1.025031205329519 2314 68-69 1.0480525618709464 2314 70-71 1.0661543190767162 2314 72-73 1.0923262448504119 2314 74-75 1.1032605083598455 2314 76-77 1.0681135341490844 2314 78-79 1.0930397795821563 2314 80-81 1.1331083908326853 2314 82-83 1.129243491510593 2314 84-85 1.1670106274978096 2314 86-87 1.207812829682883 2314 88-89 1.2114227795221062 2314 90-91 1.3050575442012011 2314 92-93 1.3812059720117063 2314 94-95 1.3410547225237401 2314 96-97 1.3712532751505826 2314 98-99 1.4589269856604012 2314 100 1.5403043631181532 2315 1 0.47050926826270967 2315 2 0.4831274948248172 2315 3 0.527652759356311 2315 4 0.25467161301839525 2315 5 0.3112982497326442 2315 6 0.37863533183774933 2315 7 0.4444607364860502 2315 8 0.4313545754902748 2315 9 0.6251532191286273 2315 10-11 0.46527354032892987 2315 12-13 0.5083422248504377 2315 14-15 0.7276496855422465 2315 16-17 0.7339163347206181 2315 18-19 0.6171568607772571 2315 20-21 0.7109339835672301 2315 22-23 0.5890358672234726 2315 24-25 0.728539242807642 2315 26-27 0.8221550365494821 2315 28-29 0.8299770472228616 2315 30-31 0.8689552752960736 2315 32-33 0.905151570856809 2315 34-35 0.9214564020102145 2315 36-37 0.9980516690646155 2315 38-39 1.0284942016518102 2315 40-41 1.0588935583766244 2315 42-43 1.1183295413568786 2315 44-45 1.1482436704110697 2315 46-47 1.1823649894325712 2315 48-49 1.214923480669647 2315 50-51 1.0513370249854503 2315 52-53 1.1047326366865136 2315 54-55 0.9957084150970203 2315 56-57 0.92540758556882 2315 58-59 0.9632864622785036 2315 60-61 0.9598092653065038 2315 62-63 0.9049718954575425 2315 64-65 0.9982126885968228 2315 66-67 0.9618922732094504 2315 68-69 0.9782042431623665 2315 70-71 0.953127421732546 2315 72-73 0.6600567506734336 2315 74-75 0.9002487185390251 2315 76-77 0.9096306326472643 2315 78-79 0.9138742755793245 2315 80-81 0.6437822049429336 2315 82-83 0.6855465148302784 2315 84-85 0.9199382321198755 2315 86-87 1.0135119013359777 2315 88-89 1.0700627992080065 2315 90-91 1.07533850570508 2315 92-93 1.1181097166233158 2315 94-95 1.16143034871779 2315 96-97 1.0558637662930614 2315 98-99 0.47764229814532655 2315 100 0.9607450331142644 2316 1 0.2904544513336944 2316 2 0.26754090945730624 2316 3 0.3305926254353757 2316 4 0.2223729574844171 2316 5 0.18384330467693388 2316 6 0.2466806773043757 2316 7 0.20920756182222533 2316 8 0.2841438956243678 2316 9 0.31951974767160607 2316 10-11 0.32043599787326826 2316 12-13 0.10928986154042519 2316 14-15 0.1489304913831191 2316 16-17 0.3111593884950423 2316 18-19 0.38566621663782996 2316 20-21 0.4009738110432224 2316 22-23 0.3820036538244125 2316 24-25 0.4303849200831138 2316 26-27 0.45651375718993137 2316 28-29 0.4153670524724191 2316 30-31 0.34842328745071427 2316 32-33 0.5567933293896985 2316 34-35 0.528431407582886 2316 36-37 0.5102050264853872 2316 38-39 0.7148559016450946 2316 40-41 0.7684185080691535 2316 42-43 0.6195009860048728 2316 44-45 0.5889102766247234 2316 46-47 0.4097563227039842 2316 48-49 0.2967082888005592 2316 50-51 0.3494459215240866 2316 52-53 0.5357112580323431 2316 54-55 0.22959985206545497 2316 56-57 0.11982670261901518 2316 58-59 0.11872480207013325 2316 60-61 0.2574350346429952 2316 62-63 0.4351427906053331 2316 64-65 0.3514728314323037 2316 66-67 0.33257406592098704 2316 68-69 0.3179545518929885 2316 70-71 0.02913559624221307 2316 72-73 -0.012657345857419955 2316 74-75 0.22664373942286886 2316 76-77 0.4168856644588814 2316 78-79 0.45817122373689045 2316 80-81 0.6292124985201148 2316 82-83 0.6046585740901378 2316 84-85 0.6310852369397111 2316 86-87 0.6966382306190688 2316 88-89 0.6561584605546109 2316 90-91 0.5065609700634575 2316 92-93 0.5588083750351842 2316 94-95 0.7268801596746393 2316 96-97 0.5970437110932778 2316 98-99 0.5006181715796103 2316 100 0.3708726030237628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 34.0 1 592.0 2 217.0 3 210.0 4 169.0 5 156.0 6 158.0 7 551.0 8 1795.0 9 3439.0 10 5505.0 11 8728.0 12 12606.0 13 16871.0 14 21026.0 15 25180.0 16 31344.0 17 40005.0 18 50341.0 19 61950.0 20 78366.0 21 98698.0 22 108571.0 23 106835.0 24 102512.0 25 103220.0 26 112467.0 27 128495.0 28 151462.0 29 179662.0 30 218708.0 31 271196.0 32 343766.0 33 452638.0 34 653939.0 35 909365.0 36 1374550.0 37 1895633.0 38 1638950.0 39 507768.0 40 554.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88657399995408 20.62028087059775 12.807811275286276 22.685333854161893 2 15.536598820969004 19.798906943019958 41.65331823224071 23.011176003770327 3 19.26011987113601 26.838593042978715 28.547678349661577 25.353608736223705 4 12.217782309286003 16.42101805178468 39.770067009672154 31.591132629257164 5 12.787311768087065 38.58410595276751 34.60426978908352 14.024312490061911 6 29.35382296574612 38.32935678800149 18.16017473120355 14.156645515048844 7 27.139392018759768 32.32307882225552 22.922357025095287 17.61517213388943 8 26.296521107646743 35.28261818044053 19.814322220202126 18.6065384917106 9 26.552494136481624 15.420548914260868 21.83356625446352 36.19339069479398 10-11 25.792152493672056 26.387034406171672 26.969898138329825 20.85091496182645 12-13 25.8778131996423 24.190580542844963 27.811743032644838 22.1198632248679 14-15 23.203027766568287 25.23329719880751 25.943102375976295 25.620572658647912 16-17 21.39694723423893 28.058405613670505 27.164720540157912 23.379926611932653 18-19 20.780765540986526 27.893188939005608 29.911381525142616 21.41466399486525 20-21 22.42877912332231 26.939295702777077 29.393495305048834 21.238429868851778 22-23 22.726283239015842 26.796374058411864 29.001990167321857 21.475352535250437 24-25 22.05682437365851 26.726840407226693 29.935149373200492 21.2811858459143 26-27 21.98987225434582 27.012809043287994 28.90757383603097 22.089744866335216 28-29 21.16966286642018 27.392799343383274 29.5084740093501 21.92906378084645 30-31 22.096460590248967 27.05618312872347 29.427352241574717 21.42000403945285 32-33 22.362482454819954 27.273486097012782 28.566339757024185 21.79769169114308 34-35 22.34411231337279 27.10060993762501 29.330448811024834 21.224828937977367 36-37 21.955194610442554 26.78778267624018 28.953705516297184 22.303317197020085 38-39 22.16546695839307 26.978787911691438 29.44171020271698 21.414034927198507 40-41 21.91545039618314 26.091813004234837 29.950580675773107 22.042155923808913 42-43 21.888931691464283 26.30481420894536 29.23919580222534 22.567058297365016 44-45 21.95971371879898 27.209725943140086 29.055719833543076 21.774840504517858 46-47 22.02184803659784 27.006582206808694 28.417549977801944 22.554019778791528 48-49 22.66861971000508 26.977413917456555 28.342560946692448 22.011405425845922 50-51 22.374616749276317 26.798453040129804 28.29711415858994 22.529816052003937 52-53 21.873394141829394 27.416126851266238 28.009882615885314 22.700596391019054 54-55 21.79702526195842 27.347522672868884 28.02945092567478 22.82600113949791 56-57 22.67390642859731 26.88931763680863 27.662818984060266 22.773956950533794 58-59 21.777265487096443 27.32255875812723 28.408896837927287 22.49127891684904 60-61 21.858459706757255 27.945972054887346 27.701973621779892 22.493594616575507 62-63 22.192130232887504 27.195195354683378 27.463722873466256 23.148951538962866 64-65 21.545584263676503 27.2932856015314 27.855719147375652 23.305410987416444 66-67 21.991423008851562 26.956756378691342 27.76732306050784 23.28449755194925 68-69 21.599298534673068 27.04920154842418 27.97277766074222 23.378722256160533 70-71 21.688185486133165 26.686438073924545 28.09285527154889 23.532521168393405 72-73 22.28696646521929 26.784207261370508 28.19440409617673 22.734422177233473 74-75 22.460196350398924 27.035655308957924 27.792091391946144 22.712056948697015 76-77 22.178868367122615 26.946009055302554 27.563132082431412 23.311990495143416 78-79 22.04625606665812 26.989168899292228 27.577266735248834 23.387308298800818 80-81 21.634952361641886 27.460551816556556 28.05071784320769 22.85377797859387 82-83 22.54068014128189 26.507022540788206 29.02827557286481 21.924021745065094 84-85 22.673200953663823 26.8636235417515 27.845716771477274 22.6174587331074 86-87 21.571997385362753 27.52123363619771 28.226391194952306 22.680377783487227 88-89 22.55724167940625 26.368897272394378 28.117959737080493 22.955901311118872 90-91 22.30769800792532 26.51079609090054 28.160617698149952 23.020888203024185 92-93 22.61655929234944 26.68128193788575 28.076753536652397 22.62540523311241 94-95 21.5574165314077 26.624105978532164 28.729576067345068 23.088901422715065 96-97 21.967974456470778 26.54706397523129 28.230413593024235 23.254547975273695 98-99 21.408821475250896 27.017661178965795 28.082533873833192 23.490983471950113 100 22.236097074145746 26.494595858397872 27.308796460554095 23.960510606902286 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1202.0 1 871.5 2 857.0 3 1273.0 4 1141.5 5 1010.0 6 1487.5 7 2705.0 8 4462.0 9 5602.0 10 5491.0 11 5337.5 12 5962.5 13 6971.5 14 8316.5 15 9835.5 16 11129.0 17 12543.5 18 13987.5 19 15090.5 20 16332.0 21 17818.0 22 19727.5 23 22069.0 24 24869.0 25 28744.5 26 34088.5 27 40459.5 28 49358.5 29 66943.5 30 92916.0 31 117631.5 32 151216.5 33 189867.5 34 221176.5 35 251200.0 36 278854.0 37 320868.0 38 337875.5 39 320930.0 40 299209.0 41 273646.5 42 264610.0 43 274519.5 44 297087.0 45 343066.0 46 385369.0 47 413747.0 48 433821.0 49 426680.0 50 409115.0 51 416211.0 52 416032.0 53 391998.5 54 365659.5 55 312568.5 56 267809.5 57 217655.5 58 168885.0 59 160040.0 60 133238.5 61 91790.0 62 68264.5 63 57763.0 64 42793.5 65 21677.5 66 12270.0 67 8324.5 68 6172.0 69 4859.5 70 3792.5 71 2858.5 72 2138.5 73 1763.0 74 1403.0 75 1009.0 76 734.5 77 499.0 78 339.0 79 257.5 80 212.5 81 169.5 82 121.0 83 93.0 84 68.5 85 58.5 86 46.5 87 31.5 88 26.0 89 18.0 90 20.5 91 17.0 92 13.0 93 10.5 94 6.0 95 3.5 96 4.5 97 6.5 98 3.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05662552612450496 2 0.1335016492711843 3 0.016782888080877262 4 0.016206651580246284 5 0.01879971583308569 6 0.051727515869141635 7 0.056913644374820445 8 0.048753723928385326 9 0.040418874544258666 10-11 0.03892683360512489 12-13 0.040058726731364305 14-15 0.01814115983236457 16-17 0.017143035893771624 18-19 0.017405429300308944 20-21 0.021094371898098336 22-23 0.025374985902785613 24-25 0.02434599215165886 26-27 0.019082689114645543 28-29 0.018079420207296963 30-31 0.016150056923934314 32-33 0.023095764744039862 34-35 0.02303402511897226 36-37 0.023234678900441973 38-39 0.02109951686685397 40-41 0.02466498021450815 42-43 0.03173931225350455 44-45 0.041231779607648796 46-47 0.04676262101995507 48-49 0.03931270626179741 50-51 0.027371233779971502 52-53 0.03910176254281643 54-55 0.03526361585111366 56-57 0.03745537254101363 58-59 0.04248715198402343 60-61 0.022493803399630714 62-63 0.025395565777808146 64-65 0.024875923933489134 66-67 0.031420324190655255 68-69 0.03818081313555799 70-71 0.03979118835607135 72-73 0.04319715767230089 74-75 0.026136441278619405 76-77 0.019921319021813846 78-79 0.019617765865231455 80-81 0.02389323490116309 82-83 0.019679505490299058 84-85 0.012152416200806896 86-87 0.014369897734485038 88-89 0.01850130764525893 90-91 0.02088857314787299 92-93 0.019664070584032155 94-95 0.023687436150937744 96-97 0.02495309846482364 98-99 0.02734550893619333 100 0.028945594219195427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9718232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.692038339184734 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97003093751952 48.386861231015445 2 14.562050599059194 18.549733701048527 3 4.895163336839374 9.35348792779635 4 1.989852249458348 5.069509830472564 5 0.9879389471762796 3.14619226501627 6 0.5383914906890483 2.0574750879874606 7 0.2968408482131938 1.3234479079521597 8 0.1899226276923421 0.967724742756749 9 0.11629945258079698 0.6666614273342085 >10 0.40876948339340624 4.328431391552871 >50 0.02422901402787082 1.0725057241718305 >100 0.018163028357933823 2.2670413047168054 >500 0.0015442347551066046 0.6901303895899048 >1k 6.979936314455547E-4 0.7534322140802993 >5k 4.23026425240403E-5 0.1961480633468721 >10k+ 6.345396378606046E-5 1.171216791161656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38325 0.39436185511932625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30433 0.31315366828040325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18181 0.18708135389235409 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.0579875022534963E-5 0.0 0.0 0.0 6 0.0 2.0579875022534963E-5 0.0 0.0 0.0 7 0.0 2.0579875022534963E-5 0.0 0.0 0.0 8 0.0 2.0579875022534963E-5 0.0 0.0 0.0 9 0.0 2.0579875022534963E-5 0.0 0.0 0.0 10-11 0.0 2.0579875022534963E-5 0.0 0.0 0.0 12-13 0.0 2.0579875022534963E-5 0.0 2.0579875022534963E-5 0.0 14-15 0.0 2.0579875022534963E-5 0.0 2.5724843778168707E-5 0.0 16-17 0.0 3.086981253380245E-5 0.0 5.1449687556337414E-5 0.0 18-19 0.0 3.086981253380245E-5 0.0 9.775440635704107E-5 0.0 20-21 0.0 3.086981253380245E-5 0.0 1.646390001802797E-4 0.0 22-23 0.0 3.086981253380245E-5 0.0 3.7043775040562934E-4 0.0 24-25 0.0 3.086981253380245E-5 0.0 8.643547509464684E-4 0.0 26-27 0.0 3.086981253380245E-5 0.0 0.001841898814516879 0.0 28-29 0.0 3.086981253380245E-5 0.0 0.0052838829120358515 0.0 30-31 0.0 3.086981253380245E-5 0.0 0.018876890364420196 0.0 32-33 0.0 3.086981253380245E-5 0.0 0.05241179671364092 0.0 34-35 0.0 3.086981253380245E-5 0.0 0.10318234839423468 0.0 36-37 0.0 4.115975004506993E-5 0.0 0.18892839767562658 0.0 38-39 0.0 5.144968755633741E-5 0.0 0.30969110430786173 0.0 40-41 0.0 5.144968755633741E-5 0.0 0.4373480690726461 0.0 42-43 0.0 5.144968755633741E-5 0.0 0.5604928962387397 0.0 44-45 0.0 5.6594656311971154E-5 0.0 0.7066563136175387 0.0 46-47 0.0 6.688459382323863E-5 0.0 0.8792700153690507 0.0 48-49 0.0 7.202956257887237E-5 0.0 1.0713985836106814 0.0 50-51 0.0 7.202956257887237E-5 0.0 1.2701230017970349 0.0 52-53 0.0 7.202956257887237E-5 0.0 1.4573175450020126 0.0 54-55 0.0 7.202956257887237E-5 0.0 1.6592627136293927 0.0 56-57 0.0 7.202956257887237E-5 0.0 1.8937703895111786 0.0 58-59 0.0 7.202956257887237E-5 0.0 2.1658209024028237 0.0 60-61 0.0 7.202956257887237E-5 0.0 2.4395692549838284 0.0 62-63 0.0 7.202956257887237E-5 0.0 2.7136005808463928 0.0 64-65 0.0 7.202956257887237E-5 0.0 2.9887123501476403 0.0 66-67 0.0 7.202956257887237E-5 0.0 3.296098508452978 5.144968755633741E-6 68-69 0.0 7.202956257887237E-5 0.0 3.634457378667231 1.0289937511267482E-5 70-71 0.0 7.717453133450611E-5 0.0 3.9986028322847202 1.0289937511267482E-5 72-73 0.0 8.231950009013985E-5 0.0 4.361925090901308 1.0289937511267482E-5 74-75 0.0 8.231950009013985E-5 0.0 4.70863939037471 1.0289937511267482E-5 76-77 0.0 8.231950009013985E-5 0.0 5.097542433644309 1.0289937511267482E-5 78-79 0.0 8.231950009013985E-5 0.0 5.51463990569478 1.0289937511267482E-5 80-81 0.0 8.231950009013985E-5 0.0 5.964577713312463 1.0289937511267482E-5 82-83 0.0 8.231950009013985E-5 0.0 6.418327942778069 1.0289937511267482E-5 84-85 0.0 8.231950009013985E-5 0.0 6.852167143159373 1.0289937511267482E-5 86-87 0.0 8.231950009013985E-5 0.0 7.306241505656585 1.0289937511267482E-5 88 0.0 8.231950009013985E-5 0.0 7.671590881962892 1.0289937511267482E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20535 0.0 48.177692 1 GTATCAA 36380 0.0 40.623276 1 TACCTGG 5290 0.0 36.47787 2 GTACTAG 9085 0.0 36.057228 1 GGGCACC 9665 0.0 34.767418 94 CAACGCA 41575 0.0 34.327198 5 ATCAACG 42170 0.0 33.865326 3 GCATCGT 9085 0.0 33.16702 8 TATCAAC 45745 0.0 32.162266 2 GTGGTAT 6125 0.0 31.889668 1 TAGGCAT 11320 0.0 31.186195 5 AACGCAG 47190 0.0 30.910439 6 CTGTGCG 12270 0.0 30.533453 9 CTAACGC 4990 0.0 29.58014 3 ACCTGGG 6435 0.0 29.512358 3 TAACGCC 4955 0.0 29.504015 4 TAGTACT 12900 0.0 29.33395 4 TCTAACG 5075 0.0 29.212978 2 TCAACGC 49450 0.0 29.107391 4 CGCCTAC 5100 0.0 28.665766 7 >>END_MODULE