FastQCFastQC Report
Mon 6 Jun 2016
ERR1211238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9718232
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346530.35657720457795206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293990.3025138728937527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177050.18218334363699076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213900.048.2286031
GTATCAA374550.038.9162251
GGGCACC97950.036.62038894
TACCTGG51850.036.1672252
GTACTAG93300.035.7812541
ATCAACG427850.032.44153
GTGGTAT66000.032.4150241
CAACGCA428350.032.3814965
ACCTGGG57200.031.5547143
CTGTGCG126000.031.1749749
TATCAAC469900.030.746062
TAGGCAT116400.030.4770765
TAGTACT134750.029.754274
AACGCAG485900.029.3004996
ACTGTGC145650.028.3885068
TCAACGC502550.027.9560414
GTACTGT149350.027.2767856
CTAGGCA133800.027.1902944
GCATCGT111650.027.1438458
ACGCAGA516950.026.9952417
Produced by FastQC (version 0.11.5)