##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211238_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9718232 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.201311102677934 33.0 31.0 34.0 30.0 34.0 2 32.34713711300574 34.0 31.0 34.0 31.0 34.0 3 32.42499232370662 34.0 31.0 34.0 31.0 34.0 4 36.02343677327316 37.0 35.0 37.0 35.0 37.0 5 35.92052854881423 37.0 35.0 37.0 35.0 37.0 6 35.95057310835963 37.0 35.0 37.0 35.0 37.0 7 35.89288987955834 37.0 35.0 37.0 35.0 37.0 8 35.88569165667171 37.0 35.0 37.0 35.0 37.0 9 37.49394406307649 39.0 37.0 39.0 35.0 39.0 10-11 37.47871541860701 39.0 37.0 39.0 35.0 39.0 12-13 37.37129068332594 39.0 37.0 39.0 34.0 39.0 14-15 38.59587685290904 40.0 38.0 41.0 34.0 41.0 16-17 38.56434514014484 40.0 38.0 41.0 34.0 41.0 18-19 38.60240010734463 40.0 38.0 41.0 34.0 41.0 20-21 38.573125595272884 40.0 38.0 41.0 34.0 41.0 22-23 38.439636654074526 40.0 38.0 41.0 34.0 41.0 24-25 38.3750860238776 40.0 38.0 41.0 34.0 41.0 26-27 38.23031648143407 40.0 38.0 41.0 34.0 41.0 28-29 38.216001686314954 40.0 38.0 41.0 34.0 41.0 30-31 38.21362635713986 40.0 37.5 41.0 34.0 41.0 32-33 38.07562363195281 40.0 37.0 41.0 33.5 41.0 34-35 37.93530140050166 40.0 37.0 41.0 33.0 41.0 36-37 37.79380467558296 40.0 37.0 41.0 33.0 41.0 38-39 37.69807198469845 40.0 37.0 41.0 33.0 41.0 40-41 37.58874042109717 40.0 36.5 41.0 33.0 41.0 42-43 37.42748007044903 40.0 36.0 41.0 32.0 41.0 44-45 37.24229659263125 40.0 36.0 41.0 31.5 41.0 46-47 36.96335238755363 39.0 35.5 41.0 31.0 41.0 48-49 36.68302217934291 39.0 35.0 41.0 30.5 41.0 50-51 36.45575033606936 39.0 35.0 41.0 30.0 41.0 52-53 36.23126475062543 39.0 35.0 40.0 30.0 41.0 54-55 35.983022940798286 38.0 35.0 40.0 29.0 41.0 56-57 35.7005260833452 38.0 35.0 40.0 28.5 41.0 58-59 35.407586997305685 37.5 34.0 40.0 28.0 41.0 60-61 35.146042099015546 37.0 34.0 40.0 28.0 41.0 62-63 34.83721267407488 36.5 34.0 40.0 27.5 41.0 64-65 34.46930172072451 36.0 33.5 39.0 26.5 41.0 66-67 34.174674621885956 35.5 33.0 39.0 26.0 40.5 68-69 33.86760580525346 35.0 33.0 38.5 26.0 40.0 70-71 33.59109481024944 35.0 33.0 38.0 26.0 40.0 72-73 33.28221110588839 35.0 33.0 37.0 26.0 40.0 74-75 32.96563644498299 35.0 32.0 37.0 25.0 39.0 76-77 31.981983296961836 34.0 30.5 35.5 24.5 38.5 78-79 32.479178568694394 35.0 32.0 36.0 25.0 38.5 80-81 32.424259268558316 35.0 32.0 35.5 25.0 37.5 82-83 32.18997354662865 35.0 32.0 35.0 25.0 37.0 84-85 31.956154216116673 34.5 32.0 35.0 24.5 37.0 86-87 31.732856809757166 34.0 31.0 35.0 24.0 36.0 88-89 31.521575529376126 34.0 31.0 35.0 24.0 36.0 90-91 31.31217339738339 34.0 31.0 35.0 23.0 35.5 92-93 31.128755518493485 34.0 31.0 35.0 23.0 35.0 94-95 30.95377116948844 34.0 31.0 35.0 21.0 35.0 96-97 30.794493226751534 34.0 30.5 35.0 20.0 35.0 98-99 30.63215212396658 34.0 30.0 35.0 20.0 35.0 100 28.834649656439566 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2980255338686639 1101 2 -0.29448253430227567 1101 3 -0.35932571506356226 1101 4 -0.15672834791106993 1101 5 -0.24654579125214582 1101 6 -0.25490406308242086 1101 7 -0.2995617528391108 1101 8 -0.3107163616362101 1101 9 -0.45087342422789334 1101 10-11 -0.40186247598472846 1101 12-13 -0.4017646053437218 1101 14-15 -0.6138952952089625 1101 16-17 -0.5953391371360439 1101 18-19 -0.65804232893273 1101 20-21 -0.67446952359834 1101 22-23 -0.6580430280875049 1101 24-25 -0.646549096253004 1101 26-27 -0.7430224130201424 1101 28-29 -0.8016349879791704 1101 30-31 -0.598312966124233 1101 32-33 -0.5515074717018749 1101 34-35 -0.5573153048717643 1101 36-37 -0.5283507150102906 1101 38-39 -0.6418389748729751 1101 40-41 -0.6945674722869839 1101 42-43 -0.6685847067097086 1101 44-45 -0.6981138154603315 1101 46-47 -0.7091555170712951 1101 48-49 -0.6904440568499979 1101 50-51 -0.690620321753066 1101 52-53 -0.8028702240706878 1101 54-55 -0.7157748107392052 1101 56-57 -0.7046118969962833 1101 58-59 -0.6727810834673349 1101 60-61 -0.753666328297605 1101 62-63 -0.7999329048119463 1101 64-65 -0.6965099696421007 1101 66-67 -0.7386814469625946 1101 68-69 -0.8965270633614395 1101 70-71 -0.9021238814162871 1101 72-73 -0.9509306714501875 1101 74-75 -0.9732770122411871 1101 76-77 -0.8708923364243759 1101 78-79 -0.8481128025690339 1101 80-81 -0.6982694764082709 1101 82-83 -0.7049318118657766 1101 84-85 -0.6836390239540187 1101 86-87 -0.7973775964686247 1101 88-89 -0.8437808338188155 1101 90-91 -0.8595209210123151 1101 92-93 -0.9294646493419343 1101 94-95 -0.9494609374613958 1101 96-97 -1.0243565655841245 1101 98-99 -1.031084706638783 1101 100 -0.8849317443996867 1102 1 -0.3912836787526359 1102 2 -0.3670989955670194 1102 3 -0.39274631492273926 1102 4 -0.28188756596335907 1102 5 -0.3045918500208771 1102 6 -0.3293232632855876 1102 7 -0.3328314483053987 1102 8 -0.34566146305633794 1102 9 -0.49346823237054593 1102 10-11 -0.543411892168919 1102 12-13 -0.5102821796349701 1102 14-15 -0.6747286508008941 1102 16-17 -0.7372854250626872 1102 18-19 -0.6561277072387313 1102 20-21 -0.7156275640870291 1102 22-23 -0.7380395018521995 1102 24-25 -0.7178515107292185 1102 26-27 -0.8178703034057406 1102 28-29 -0.8601909931418987 1102 30-31 -0.7585363493403108 1102 32-33 -0.6512037943785813 1102 34-35 -0.6952963030000845 1102 36-37 -0.839585428611457 1102 38-39 -0.8195155719528557 1102 40-41 -0.8090111088743868 1102 42-43 -0.7510077473941337 1102 44-45 -0.8008593189328792 1102 46-47 -0.7871382492972003 1102 48-49 -0.8861684832193362 1102 50-51 -0.8961948206400265 1102 52-53 -0.8588357136929616 1102 54-55 -0.8846479532100702 1102 56-57 -0.8834137401027391 1102 58-59 -0.8691598311065718 1102 60-61 -0.8768632043989939 1102 62-63 -0.9918059747918448 1102 64-65 -0.9211088899886875 1102 66-67 -0.9835825604628923 1102 68-69 -1.0770793944641568 1102 70-71 -0.9926226242843939 1102 72-73 -1.0220743490743445 1102 74-75 -1.1236563268379314 1102 76-77 -1.0378930917754872 1102 78-79 -0.8568194693036126 1102 80-81 -0.8221202962224439 1102 82-83 -0.8058831047486379 1102 84-85 -0.8909234345530272 1102 86-87 -0.8825689154624463 1102 88-89 -0.9355245453313152 1102 90-91 -0.9318064941910826 1102 92-93 -1.0394365513940862 1102 94-95 -1.0646478803462784 1102 96-97 -1.0587368415745182 1102 98-99 -1.1563823845056405 1102 100 -1.277411342842388 1103 1 -0.42301685393395516 1103 2 -0.3620864208654133 1103 3 -0.38864483539003203 1103 4 -0.2516725032139533 1103 5 -0.23052427305412948 1103 6 -0.3365738832538412 1103 7 -0.406948950634586 1103 8 -0.33754158958553404 1103 9 -0.4765320920895064 1103 10-11 -0.5062059124198299 1103 12-13 -0.6030765921159187 1103 14-15 -0.703554328285783 1103 16-17 -0.707497190005661 1103 18-19 -0.6654606617009975 1103 20-21 -0.6484009430184798 1103 22-23 -0.7298185252529024 1103 24-25 -0.8199357266750269 1103 26-27 -0.8085411630817774 1103 28-29 -0.9035226355405896 1103 30-31 -0.7685320530266253 1103 32-33 -0.7268077005571527 1103 34-35 -0.8137158185908646 1103 36-37 -0.7914024157814907 1103 38-39 -0.7817119110404533 1103 40-41 -0.8146280110175965 1103 42-43 -0.7362297262578892 1103 44-45 -0.7452507196563758 1103 46-47 -0.7996160034779223 1103 48-49 -0.7802902670610763 1103 50-51 -0.8517236190350346 1103 52-53 -0.8492993574975287 1103 54-55 -0.8623968823765082 1103 56-57 -0.8981820978065187 1103 58-59 -0.8541046302785276 1103 60-61 -0.87325743475769 1103 62-63 -0.812915289020026 1103 64-65 -0.7810972528819704 1103 66-67 -0.9047892092232246 1103 68-69 -0.8046930071123342 1103 70-71 -0.8273926875341857 1103 72-73 -0.8024987340103849 1103 74-75 -0.868992589239717 1103 76-77 -0.8584086670653832 1103 78-79 -0.7107509378914507 1103 80-81 -0.7156803770411884 1103 82-83 -0.685515560720102 1103 84-85 -0.7346769604961665 1103 86-87 -0.7855699136281338 1103 88-89 -0.7812857738982757 1103 90-91 -0.7216516868568696 1103 92-93 -0.9027244854757548 1103 94-95 -0.8708419498508064 1103 96-97 -1.056605136760588 1103 98-99 -1.0528567231539832 1103 100 -1.2073235060838634 1104 1 -0.3580925949353073 1104 2 -0.33253935814329694 1104 3 -0.4293297483623597 1104 4 -0.23213181101820624 1104 5 -0.2991462526282831 1104 6 -0.3227794368212784 1104 7 -0.32473458088693263 1104 8 -0.30656064890801105 1104 9 -0.5105048132580876 1104 10-11 -0.505859416710706 1104 12-13 -0.5880430819732894 1104 14-15 -0.769280113797663 1104 16-17 -0.6824697348930329 1104 18-19 -0.7250651588909705 1104 20-21 -0.6944433428484516 1104 22-23 -0.675706178331481 1104 24-25 -0.6665072391691851 1104 26-27 -0.786028025077016 1104 28-29 -0.8741037890708228 1104 30-31 -0.8501467826792677 1104 32-33 -0.7665630609856535 1104 34-35 -0.768867495027358 1104 36-37 -0.7003656360530925 1104 38-39 -0.7154233612782974 1104 40-41 -0.7423033651024795 1104 42-43 -0.6894640286088958 1104 44-45 -0.8587770634799625 1104 46-47 -0.8631875344779445 1104 48-49 -0.9155823064955655 1104 50-51 -0.8395677223659916 1104 52-53 -0.7881730319678439 1104 54-55 -0.7930227592138479 1104 56-57 -0.8052547312910363 1104 58-59 -0.803437552497364 1104 60-61 -0.7183720660521047 1104 62-63 -0.8117349155130995 1104 64-65 -0.8009020204205299 1104 66-67 -0.8502457315017153 1104 68-69 -0.8139849455118977 1104 70-71 -0.9216313544688717 1104 72-73 -0.9006156895156465 1104 74-75 -0.834285306407125 1104 76-77 -0.7947988743193122 1104 78-79 -0.6410716718691347 1104 80-81 -0.6424806974941859 1104 82-83 -0.6822998009134338 1104 84-85 -0.6863561185268772 1104 86-87 -0.6979653049138612 1104 88-89 -0.790654133885436 1104 90-91 -0.742747238040284 1104 92-93 -0.7414421699596687 1104 94-95 -0.7909769613675124 1104 96-97 -0.8340517165830512 1104 98-99 -0.8336505535670007 1104 100 -1.0821432200245908 1105 1 -0.3739273568442485 1105 2 -0.3484947181607687 1105 3 -0.4101045416255005 1105 4 -0.1723874190886363 1105 5 -0.2628831157191911 1105 6 -0.35912827976301287 1105 7 -0.3525294750864276 1105 8 -0.38302556694048207 1105 9 -0.48950162826701415 1105 10-11 -0.5144759278477196 1105 12-13 -0.5369351656755939 1105 14-15 -0.6637462436461874 1105 16-17 -0.6593448775047435 1105 18-19 -0.6263791562665162 1105 20-21 -0.6870250415582504 1105 22-23 -0.6901317033280279 1105 24-25 -0.7901530926842426 1105 26-27 -0.8231813154907002 1105 28-29 -0.7452419626986782 1105 30-31 -0.7043977148975316 1105 32-33 -0.7364182240702632 1105 34-35 -0.7176030484552669 1105 36-37 -0.6861473898732555 1105 38-39 -0.7300265781288289 1105 40-41 -0.7708613321245039 1105 42-43 -0.741595445799021 1105 44-45 -0.7982601790448385 1105 46-47 -0.7731492064418504 1105 48-49 -0.7946353229966405 1105 50-51 -0.7853623591726446 1105 52-53 -0.8782402993634619 1105 54-55 -0.7455075819785222 1105 56-57 -0.8292446426535136 1105 58-59 -0.9207278085038197 1105 60-61 -0.7926746435141752 1105 62-63 -0.8454923055324457 1105 64-65 -0.897846225531616 1105 66-67 -0.8343396874497557 1105 68-69 -0.7792448208949949 1105 70-71 -0.8268666023523394 1105 72-73 -0.842123121128779 1105 74-75 -0.7820082896997604 1105 76-77 -0.7361085776276894 1105 78-79 -0.6948813952924553 1105 80-81 -0.7258042110260092 1105 82-83 -0.6853846751488213 1105 84-85 -0.7392544222028867 1105 86-87 -0.734769026965548 1105 88-89 -0.7524682695929492 1105 90-91 -0.7632490473193592 1105 92-93 -0.6940407353968929 1105 94-95 -0.7501153379895413 1105 96-97 -0.8576757779230881 1105 98-99 -0.8861004149164629 1105 100 -0.928563779068913 1106 1 -0.3786123312257992 1106 2 -0.3625228923498156 1106 3 -0.4297996874351746 1106 4 -0.2923602293262775 1106 5 -0.2674764223315407 1106 6 -0.31580658043041865 1106 7 -0.344176612782249 1106 8 -0.36186562778433284 1106 9 -0.5351528786147597 1106 10-11 -0.5472559839967346 1106 12-13 -0.5630022671088 1106 14-15 -0.6725282288256906 1106 16-17 -0.6623137360725835 1106 18-19 -0.6524230464688188 1106 20-21 -0.7422571516757799 1106 22-23 -0.6721367441787365 1106 24-25 -0.7161438481399998 1106 26-27 -0.6838032963322576 1106 28-29 -0.7397844744719535 1106 30-31 -0.8074462755851997 1106 32-33 -0.7899415254974897 1106 34-35 -0.7808931499579046 1106 36-37 -0.7945871424529258 1106 38-39 -0.7895732033680076 1106 40-41 -0.8719829912209676 1106 42-43 -0.7363840454592676 1106 44-45 -0.8343690212464665 1106 46-47 -0.8173391322917638 1106 48-49 -0.8689981020753805 1106 50-51 -0.9093649133472681 1106 52-53 -0.830625849742944 1106 54-55 -0.8521414607204747 1106 56-57 -0.8349785492777428 1106 58-59 -0.8375726940527883 1106 60-61 -0.8940846678731305 1106 62-63 -0.8431854773543463 1106 64-65 -0.8528942174282861 1106 66-67 -0.9133178698319142 1106 68-69 -0.9027546107184534 1106 70-71 -0.8484380133290657 1106 72-73 -0.9254219861703419 1106 74-75 -0.9344664917955114 1106 76-77 -0.9219620430669657 1106 78-79 -0.7325154727105243 1106 80-81 -0.6640829057145439 1106 82-83 -0.7511458178702171 1106 84-85 -0.7146406368606257 1106 86-87 -0.6960859045985295 1106 88-89 -0.7853714448229674 1106 90-91 -0.7862537064088677 1106 92-93 -0.7574269064748727 1106 94-95 -0.8224177211574357 1106 96-97 -0.8542246179031601 1106 98-99 -0.8479897214516647 1106 100 -0.8935206936520217 1107 1 -0.37746363161508967 1107 2 -0.401290396340368 1107 3 -0.42089985048422207 1107 4 -0.24048930130567925 1107 5 -0.28989074772237444 1107 6 -0.3650702599029927 1107 7 -0.38773969096159533 1107 8 -0.4399080110834319 1107 9 -0.4543758563885092 1107 10-11 -0.5119817593660159 1107 12-13 -0.49730771035907395 1107 14-15 -0.6201352425050857 1107 16-17 -0.6549193626976475 1107 18-19 -0.669976093542104 1107 20-21 -0.7044733188502477 1107 22-23 -0.6724364601797248 1107 24-25 -0.6963624443582859 1107 26-27 -0.7176469199149551 1107 28-29 -0.7747184202560007 1107 30-31 -0.7689352067734205 1107 32-33 -0.7030693817732541 1107 34-35 -0.7338248569564954 1107 36-37 -0.7604859610489711 1107 38-39 -0.7934507809773166 1107 40-41 -0.7883447964418977 1107 42-43 -0.6994042019142697 1107 44-45 -0.7557683891987494 1107 46-47 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2208 72-73 1.1430576598649438 2208 74-75 1.1435727499520425 2208 76-77 1.2049403198050896 2208 78-79 1.0226733506946246 2208 80-81 0.9949146952194567 2208 82-83 0.9682779389496048 2208 84-85 0.9764027668662472 2208 86-87 1.0138627722952798 2208 88-89 1.0075955555746212 2208 90-91 1.0412328923692264 2208 92-93 1.1031655748006663 2208 94-95 1.1086863200372576 2208 96-97 1.2612077090280884 2208 98-99 1.244031263348223 2208 100 1.5431648096113513 2209 1 0.6598950557834229 2209 2 0.6009455276042033 2209 3 0.6115981158802768 2209 4 0.3727877111357998 2209 5 0.4526725244943819 2209 6 0.3168466798277123 2209 7 0.4585411418396248 2209 8 0.5055254715804764 2209 9 0.7103567607089118 2209 10-11 0.6580967521311862 2209 12-13 0.6999415001651457 2209 14-15 0.9426470414433368 2209 16-17 0.9271693760472957 2209 18-19 0.9128912612386912 2209 20-21 0.892039579122013 2209 22-23 0.9440808155145675 2209 24-25 0.9540100123289648 2209 26-27 0.9644754731721079 2209 28-29 0.9635954899557717 2209 30-31 0.9259277103694927 2209 32-33 0.9544183711144782 2209 34-35 0.9859999423556403 2209 36-37 1.0794654636313794 2209 38-39 1.0070828324142553 2209 40-41 1.0488260257991513 2209 42-43 1.0933100074901532 2209 44-45 1.0895099404248754 2209 46-47 1.0961688829173895 2209 48-49 1.034125954847248 2209 50-51 1.073801468079317 2209 52-53 1.0652561442059323 2209 54-55 1.0798667315042252 2209 56-57 1.1116574711839107 2209 58-59 1.05095525561957 2209 60-61 1.053923540274262 2209 62-63 1.120139862290337 2209 64-65 1.1491468725695029 2209 66-67 1.1311242276741638 2209 68-69 1.1128586082003906 2209 70-71 1.150898234354372 2209 72-73 1.189488718630173 2209 74-75 1.1639390496991453 2209 76-77 1.1676748474204999 2209 78-79 1.037679488205491 2209 80-81 0.9605315847804832 2209 82-83 1.0048104255787749 2209 84-85 0.9638614658054117 2209 86-87 1.0013340471805954 2209 88-89 1.040453493644538 2209 90-91 1.0517075571661572 2209 92-93 1.137663257008878 2209 94-95 1.1761446588470506 2209 96-97 1.2198354021843123 2209 98-99 1.2230939993130896 2209 100 1.4266511918011346 2210 1 0.6420637272412222 2210 2 0.6116455448492033 2210 3 0.59650528350695 2210 4 0.3420183103922909 2210 5 0.446505251850958 2210 6 0.45850026863681137 2210 7 0.47730607809797476 2210 8 0.5084334154722612 2210 9 0.6684413265312514 2210 10-11 0.6516721855498773 2210 12-13 0.6834445334114818 2210 14-15 0.8854851310817864 2210 16-17 0.9138670332734122 2210 18-19 0.8676283278500208 2210 20-21 0.9017510232201147 2210 22-23 0.9760235126419516 2210 24-25 0.9215752803253636 2210 26-27 0.9973242532685944 2210 28-29 0.9900412957463374 2210 30-31 0.9764936009151555 2210 32-33 0.9803111960902342 2210 34-35 1.0050668222069916 2210 36-37 1.0423843093405196 2210 38-39 1.0335664306912946 2210 40-41 1.0846277957412624 2210 42-43 1.1206386827524142 2210 44-45 1.1296458852946785 2210 46-47 1.2157982187255527 2210 48-49 1.1619158137312837 2210 50-51 1.2095322765334942 2210 52-53 1.1977645997794042 2210 54-55 1.207400509364625 2210 56-57 1.2591741073235667 2210 58-59 1.2177724437432929 2210 60-61 1.201158792317841 2210 62-63 1.240235677712171 2210 64-65 1.3114746927872716 2210 66-67 1.2999563305600361 2210 68-69 1.313307842815476 2210 70-71 1.3144027615267717 2210 72-73 1.3411841926106405 2210 74-75 1.37979025370268 2210 76-77 1.364519156787125 2210 78-79 1.1781165960799242 2210 80-81 1.0676515835507558 2210 82-83 1.1063091072250515 2210 84-85 1.1950634566179978 2210 86-87 1.22063326615989 2210 88-89 1.2297345981698804 2210 90-91 1.3041973578783406 2210 92-93 1.313537527999376 2210 94-95 1.3092109228742146 2210 96-97 1.3446805694776138 2210 98-99 1.379140713084059 2210 100 1.5174835295840623 2211 1 0.6416510979348828 2211 2 0.601118836021989 2211 3 0.5556574755482586 2211 4 0.3512211952540625 2211 5 0.42349049764307267 2211 6 0.46106701371915193 2211 7 0.4987371791786259 2211 8 0.4960074642667749 2211 9 0.6434160498114494 2211 10-11 0.6686774679607481 2211 12-13 0.723064430798054 2211 14-15 0.9084039215643784 2211 16-17 0.84967876512804 2211 18-19 0.8805570645511054 2211 20-21 0.8835182290212558 2211 22-23 0.9490705559758013 2211 24-25 0.94414432163002 2211 26-27 0.9672242392902461 2211 28-29 0.9309534264253756 2211 30-31 0.9862479539111888 2211 32-33 0.9695470790619751 2211 34-35 1.0390267414429033 2211 36-37 1.0854309317441562 2211 38-39 1.0684252713268378 2211 40-41 1.031089607846745 2211 42-43 1.0480580681899738 2211 44-45 1.071747248355912 2211 46-47 1.1102302265251822 2211 48-49 1.1011336615934724 2211 50-51 1.125395277006426 2211 52-53 1.1422202101797865 2211 54-55 1.1102515666319803 2211 56-57 1.117991741152501 2211 58-59 1.1996425474677395 2211 60-61 1.2252368057081284 2211 62-63 1.2254776639551537 2211 64-65 1.2130963721117354 2211 66-67 1.2651534590850204 2211 68-69 1.2764916573395624 2211 70-71 1.3044363908771857 2211 72-73 1.320347578019451 2211 74-75 1.294252673942367 2211 76-77 1.3057528391208493 2211 78-79 1.150714019974643 2211 80-81 1.0650113924961317 2211 82-83 1.1032058232477269 2211 84-85 1.1062081631635188 2211 86-87 1.1218796625063554 2211 88-89 1.2068234194311849 2211 90-91 1.2041176111166472 2211 92-93 1.2066524989073422 2211 94-95 1.249202059645036 2211 96-97 1.236395010484305 2211 98-99 1.3129719667464883 2211 100 1.5157695476050144 2212 1 0.1142110155514473 2212 2 0.3545878456915261 2212 3 0.47069626609553694 2212 4 0.2999962509996834 2212 5 0.38517788427179056 2212 6 0.4476677102390241 2212 7 0.4692187968341912 2212 8 0.47297457472009796 2212 9 0.6560415399418176 2212 10-11 0.6353342034817331 2212 12-13 0.6767766155491657 2212 14-15 0.9033298906136693 2212 16-17 0.5459894202002928 2212 18-19 0.7129483760027995 2212 20-21 0.8120509389518347 2212 22-23 0.898071657214679 2212 24-25 0.9172998027271362 2212 26-27 0.9869662927214407 2212 28-29 0.9936902402664103 2212 30-31 1.0139204810929527 2212 32-33 1.0475936513950117 2212 34-35 0.9154907518591742 2212 36-37 0.9764936406973845 2212 38-39 1.0635123733141683 2212 40-41 1.0978996631228242 2212 42-43 1.0964221135838983 2212 44-45 1.0611209659029726 2212 46-47 1.138925775130602 2212 48-49 1.147824603279986 2212 50-51 1.1172680590599668 2212 52-53 1.139259896868026 2212 54-55 1.151829914016325 2212 56-57 1.181738595395224 2212 58-59 1.1859409950186688 2212 60-61 1.1356434710098569 2212 62-63 1.1447789575253537 2212 64-65 1.2225597939929571 2212 66-67 1.234765731705778 2212 68-69 1.224269013402683 2212 70-71 1.2047885603819424 2212 72-73 1.1890884611037507 2212 74-75 1.20594337339341 2212 76-77 1.1467487738012991 2212 78-79 1.0136944973142548 2212 80-81 0.9453956603790417 2212 82-83 0.8575847254976097 2212 84-85 0.9938875159728688 2212 86-87 1.0246707341155137 2212 88-89 1.0618747594661322 2212 90-91 1.1109735183255829 2212 92-93 1.1767289165866366 2212 94-95 1.2375981204755604 2212 96-97 1.1855651888828582 2212 98-99 1.257533420732333 2212 100 1.3920743162100528 2213 1 0.4199603186701424 2213 2 0.4524774002787879 2213 3 0.4613737400812852 2213 4 0.24058706007663488 2213 5 0.3740556304740892 2213 6 0.40767691183506116 2213 7 0.46421595667187887 2213 8 0.4434925806737411 2213 9 0.5868220708715199 2213 10-11 0.6218693980435503 2213 12-13 0.5770247902161074 2213 14-15 0.8134558538720924 2213 16-17 0.7461972632813811 2213 18-19 0.780351253325513 2213 20-21 0.8426198398688527 2213 22-23 0.9096413810523885 2213 24-25 0.7919477702053186 2213 26-27 0.8903055366667658 2213 28-29 0.904838554966112 2213 30-31 0.905100857849483 2213 32-33 0.8946781645871766 2213 34-35 0.9351621111185509 2213 36-37 0.9851963377874853 2213 38-39 0.996135497938738 2213 40-41 0.98807807876846 2213 42-43 1.0464586786175545 2213 44-45 0.9864323793436398 2213 46-47 0.9891800730832685 2213 48-49 0.9669335285923211 2213 50-51 0.884286673819453 2213 52-53 0.9525546157265978 2213 54-55 1.0105990945738128 2213 56-57 1.0573340126941275 2213 58-59 1.0128287058222227 2213 60-61 1.0325221171961445 2213 62-63 1.0368401507009608 2213 64-65 1.0526506899589947 2213 66-67 1.0535038899932019 2213 68-69 1.0925716541757353 2213 70-71 1.1187225370234728 2213 72-73 1.0808134776685705 2213 74-75 1.0537419775724857 2213 76-77 1.0451894837862739 2213 78-79 0.9593972419175003 2213 80-81 0.8759670585019705 2213 82-83 0.666622068559029 2213 84-85 0.5756542838407874 2213 86-87 0.7384720276783661 2213 88-89 0.7498614762521818 2213 90-91 0.9543532871700542 2213 92-93 1.0562407075180822 2213 94-95 1.107178255660358 2213 96-97 1.1152445509920952 2213 98-99 1.194546310914749 2213 100 1.3013515970164384 2214 1 0.4143394641069449 2214 2 0.41115725037794704 2214 3 0.4445706012208319 2214 4 0.3007955114485128 2214 5 0.39650797022428463 2214 6 0.3918915939344245 2214 7 0.4525398315532456 2214 8 0.4401414813656288 2214 9 0.5715017351827143 2214 10-11 0.607352706326239 2214 12-13 0.6540469249403955 2214 14-15 0.821960477567778 2214 16-17 0.7484282984789772 2214 18-19 0.7430406144710346 2214 20-21 0.8125823817351758 2214 22-23 0.9112112296454313 2214 24-25 0.9178299215480621 2214 26-27 0.9608244947241076 2214 28-29 0.8267727289900151 2214 30-31 0.7794687006154888 2214 32-33 0.8598536778691397 2214 34-35 0.9001114941419743 2214 36-37 0.9041159824406719 2214 38-39 0.9285987100419959 2214 40-41 0.8143664534618082 2214 42-43 0.8396072519606008 2214 44-45 0.8294880330867755 2214 46-47 0.8399283392614194 2214 48-49 0.8108908004325741 2214 50-51 0.8268035680593115 2214 52-53 0.8924647578714655 2214 54-55 0.7980503978047153 2214 56-57 0.8080115496647338 2214 58-59 0.8809539959024733 2214 60-61 0.9173403625995178 2214 62-63 0.9301700210011461 2214 64-65 0.9205351643344315 2214 66-67 0.9240112960686702 2214 68-69 0.9779754390592146 2214 70-71 0.9486701492552925 2214 72-73 0.8866130080075081 2214 74-75 0.9164444531630522 2214 76-77 0.8735802679515388 2214 78-79 0.7878053583245972 2214 80-81 0.7781305662608347 2214 82-83 0.840298687860006 2214 84-85 0.7914840252164232 2214 86-87 0.9119265296043828 2214 88-89 0.799666484262584 2214 90-91 0.897651967972994 2214 92-93 0.9821274437937682 2214 94-95 0.9787821181376408 2214 96-97 0.9631071696667242 2214 98-99 1.113339635115782 2214 100 1.1980416169579087 2215 1 0.564596559164201 2215 2 0.5069035177384933 2215 3 0.4832645421266477 2215 4 0.28811200361560907 2215 5 0.3665686678751996 2215 6 0.39355826420800355 2215 7 0.3915942748762191 2215 8 0.4172520111536926 2215 9 0.5589615465656905 2215 10-11 0.5750324682434922 2215 12-13 0.5883090808265266 2215 14-15 0.7917342732049804 2215 16-17 0.8062452484185485 2215 18-19 0.7787727474577295 2215 20-21 0.73880408822464 2215 22-23 0.8264940217229437 2215 24-25 0.820741356182431 2215 26-27 0.8496310836180072 2215 28-29 0.8473568351872416 2215 30-31 0.5240487413247479 2215 32-33 0.655670844611798 2215 34-35 0.7718186064874288 2215 36-37 0.855253646519742 2215 38-39 0.8384262020386828 2215 40-41 0.8280956671813229 2215 42-43 0.8618504747276887 2215 44-45 0.8361460853404807 2215 46-47 0.811528244505098 2215 48-49 0.7786003124280754 2215 50-51 0.819006883409692 2215 52-53 0.8204532472200725 2215 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5 0.33193641880708924 2310 6 0.39294831060635005 2310 7 0.44585224911622134 2310 8 0.43039582967367096 2310 9 0.6026689426586458 2310 10-11 0.6114253024122576 2310 12-13 0.6597704000934002 2310 14-15 0.8317892101034445 2310 16-17 0.6434517335471526 2310 18-19 0.6938459947954314 2310 20-21 0.7973028740395662 2310 22-23 0.8515028411674663 2310 24-25 0.8818315742074319 2310 26-27 0.9068478670897591 2310 28-29 0.8967193982543549 2310 30-31 0.6969788617833714 2310 32-33 0.8595481038387263 2310 34-35 0.9111400282689459 2310 36-37 0.8070967932290856 2310 38-39 0.8777228229692469 2310 40-41 0.9026558278473829 2310 42-43 0.9902134957883248 2310 44-45 0.9931576534901794 2310 46-47 0.6147114445143274 2310 48-49 0.871569140965498 2310 50-51 0.941979034353345 2310 52-53 1.0141931865238405 2310 54-55 1.051388377842919 2310 56-57 1.0972019369199941 2310 58-59 1.0561826473727436 2310 60-61 1.0191986414600294 2310 62-63 0.4118781385146306 2310 64-65 0.5815718497894551 2310 66-67 0.9672118120208708 2310 68-69 1.0651780555459496 2310 70-71 1.062501374216268 2310 72-73 1.1118580852995734 2310 74-75 1.0921641437708942 2310 76-77 1.1079752109930112 2310 78-79 0.9800851213090525 2310 80-81 0.922945996904005 2310 82-83 0.9199798455277417 2310 84-85 0.9232893764289294 2310 86-87 0.9210669227939938 2310 88-89 0.9510253711136407 2310 90-91 1.0999360417559565 2310 92-93 1.0743208116582785 2310 94-95 1.0972422785217404 2310 96-97 1.1417155655346285 2310 98-99 1.1682665978727265 2310 100 1.3571649461036088 2311 1 0.3981976129717353 2311 2 0.3043602445105549 2311 3 0.3883107919729696 2311 4 0.27874515008872436 2311 5 0.38215687519589636 2311 6 0.4347227868320189 2311 7 0.4718623352714957 2311 8 0.4477419829900384 2311 9 0.653816121281956 2311 10-11 0.6228193682072458 2311 12-13 0.6933651729239827 2311 14-15 0.7930195784409548 2311 16-17 0.6599914522399999 2311 18-19 0.7874476467598726 2311 20-21 0.7430884038746726 2311 22-23 0.8774844870873508 2311 24-25 0.8710462671946857 2311 26-27 0.2690511386081198 2311 28-29 0.639300199718015 2311 30-31 0.8384107466093269 2311 32-33 0.9009491014346835 2311 34-35 0.9523300171440283 2311 36-37 0.9763934240740184 2311 38-39 1.0107756594168222 2311 40-41 0.99463222569306 2311 42-43 0.9606585368565135 2311 44-45 0.9480381599370347 2311 46-47 0.6622880042505699 2311 48-49 0.863148720552374 2311 50-51 0.9533290582966245 2311 52-53 0.9093537644683067 2311 54-55 0.9353474044576302 2311 56-57 1.018990426646269 2311 58-59 1.0406806367048134 2311 60-61 1.0659487530737763 2311 62-63 1.0633380949062783 2311 64-65 1.1262120321958946 2311 66-67 1.1382759953931298 2311 68-69 1.1432909567983742 2311 70-71 1.1129864787442614 2311 72-73 1.1411119383032542 2311 74-75 1.112565722325492 2311 76-77 1.06267150817159 2311 78-79 0.7723813867253213 2311 80-81 0.7040814024042277 2311 82-83 0.8518110604281546 2311 84-85 1.012672903941823 2311 86-87 0.9656921598972339 2311 88-89 0.982365874465291 2311 90-91 1.0764393512756563 2311 92-93 1.0927473900710787 2311 94-95 1.0844594784630743 2311 96-97 1.133798294209111 2311 98-99 1.2010066362954035 2311 100 1.360244958776125 2312 1 0.2698538272075979 2312 2 0.3625269081988449 2312 3 0.46191482174434384 2312 4 0.192339273185695 2312 5 0.3354774296072236 2312 6 0.40333812269621916 2312 7 0.32019573740117835 2312 8 0.4180911127204041 2312 9 0.6330136466772487 2312 10-11 0.6285552848602975 2312 12-13 0.7167003966887222 2312 14-15 0.9028421178367196 2312 16-17 0.8644743451047034 2312 18-19 0.7966021966694043 2312 20-21 0.8333471962369643 2312 22-23 0.9157194586295745 2312 24-25 0.9134488031120398 2312 26-27 0.9719019376987035 2312 28-29 0.9769131694072684 2312 30-31 0.939230790711143 2312 32-33 0.8274008376070512 2312 34-35 0.9188647563223853 2312 36-37 1.0004374023085347 2312 38-39 0.8158452918102412 2312 40-41 0.9519821644936641 2312 42-43 1.0787477127827856 2312 44-45 0.742850303742884 2312 46-47 0.7753408626256473 2312 48-49 0.9932426130976779 2312 50-51 0.8066716107603398 2312 52-53 0.9689866556089157 2312 54-55 0.8688652913408248 2312 56-57 0.9218706456149803 2312 58-59 1.0174937236079487 2312 60-61 1.0418441952484798 2312 62-63 1.0754814634373346 2312 64-65 1.0508948789250923 2312 66-67 1.0803887906584677 2312 68-69 1.1422521056957393 2312 70-71 0.9904883167394303 2312 72-73 1.0055250924338424 2312 74-75 1.0775937742469424 2312 76-77 1.054757652192393 2312 78-79 0.95151193321081 2312 80-81 0.9629695342536095 2312 82-83 0.9820315864987421 2312 84-85 1.0693539440117483 2312 86-87 1.095683282651148 2312 88-89 1.038404512886526 2312 90-91 1.1292609947667565 2312 92-93 1.1511204900018903 2312 94-95 1.1499903457073444 2312 96-97 0.8317414542872257 2312 98-99 0.6848683969097742 2312 100 0.8302442968210002 2313 1 0.04326551659622169 2313 2 0.21006823032340805 2313 3 0.23107549692112883 2313 4 0.18549055131596504 2313 5 0.3325371028051407 2313 6 0.2507642597036366 2313 7 0.2410708913486701 2313 8 0.33848637798639203 2313 9 0.556531017338429 2313 10-11 0.3602487057739694 2313 12-13 0.40304736215922077 2313 14-15 0.43578177012470576 2313 16-17 0.7006195702104705 2313 18-19 0.6382700392981562 2313 20-21 0.6247479956249364 2313 22-23 0.6038567487277362 2313 24-25 0.47789055689604965 2313 26-27 0.6940413322512882 2313 28-29 0.608579535796828 2313 30-31 0.8337019568602386 2313 32-33 0.9078778514680224 2313 34-35 0.9102426022615902 2313 36-37 0.9739783735674337 2313 38-39 0.9393048184492585 2313 40-41 0.9504879197556946 2313 42-43 0.9771959901240592 2313 44-45 0.7959038742152629 2313 46-47 0.8279750066184306 2313 48-49 0.9296817177867069 2313 50-51 0.8744955128776937 2313 52-53 0.9093790859926969 2313 54-55 0.8391003558086965 2313 56-57 0.6188692464419674 2313 58-59 0.7020026307442109 2313 60-61 0.5696006688828135 2313 62-63 0.870426656947437 2313 64-65 0.9385091728256825 2313 66-67 0.9260903866535131 2313 68-69 1.0066782798310285 2313 70-71 0.889982438556082 2313 72-73 0.9429443393388368 2313 74-75 1.048588953985714 2313 76-77 0.9819601996073288 2313 78-79 0.8489161817917434 2313 80-81 0.7865450151925231 2313 82-83 0.875064488143046 2313 84-85 0.8202216709080865 2313 86-87 0.9051605682407207 2313 88-89 0.8811827706639228 2313 90-91 0.7801160412416728 2313 92-93 0.9946310393433357 2313 94-95 1.1151357131119255 2313 96-97 1.1656346971942533 2313 98-99 1.1686363111021159 2313 100 1.1894951394459135 2314 1 -0.38735026857560584 2314 2 0.04871596519353005 2314 3 0.2903142173599136 2314 4 0.23074735285555903 2314 5 0.3211242309904918 2314 6 0.39653674121203863 2314 7 0.42405287901493693 2314 8 0.4397260148568307 2314 9 0.4291873138728377 2314 10-11 0.5775132460372703 2314 12-13 0.4373394501154806 2314 14-15 0.772985184754738 2314 16-17 0.7792624983246483 2314 18-19 0.8099914798656087 2314 20-21 0.8383412838130297 2314 22-23 0.9097231383686548 2314 24-25 0.8242636564754022 2314 26-27 0.7377005064482773 2314 28-29 0.7818128073858759 2314 30-31 0.8405427389671232 2314 32-33 0.8675290099739996 2314 34-35 0.8689543361547294 2314 36-37 0.8382524794649839 2314 38-39 0.6301666100457481 2314 40-41 0.6124658775549179 2314 42-43 0.812057719928788 2314 44-45 0.8503246599398153 2314 46-47 0.8214316320369122 2314 48-49 0.826619344595386 2314 50-51 0.7599441018618194 2314 52-53 0.8337152181055174 2314 54-55 0.745601688622564 2314 56-57 0.7406593865153397 2314 58-59 0.8518798415387963 2314 60-61 0.9017784050570654 2314 62-63 0.8416121635812388 2314 64-65 0.8504981797360927 2314 66-67 0.5666274044993145 2314 68-69 0.5268550999388211 2314 70-71 0.7125411483032877 2314 72-73 0.901600876977966 2314 74-75 0.9493227712741401 2314 76-77 0.8303076517363337 2314 78-79 0.7389072172393583 2314 80-81 0.7895119434917746 2314 82-83 0.8021753367487463 2314 84-85 0.8518821567556394 2314 86-87 0.9232793868509752 2314 88-89 0.8889779834315341 2314 90-91 0.9466750982205703 2314 92-93 0.9138127857368907 2314 94-95 0.8993658942731599 2314 96-97 0.9146160224616438 2314 98-99 1.0606658610013895 2314 100 1.1314456108691822 2315 1 0.3757198229742471 2315 2 0.39343902268883113 2315 3 0.418498163512119 2315 4 0.2569366427330664 2315 5 0.3321920488572445 2315 6 0.3913972216616415 2315 7 0.27427285835519655 2315 8 0.18614994809076535 2315 9 0.38626094597326244 2315 10-11 0.49246161436091995 2315 12-13 0.3271681903105659 2315 14-15 0.5351019305200069 2315 16-17 0.7229400137219173 2315 18-19 0.653067335686643 2315 20-21 0.41615930826355907 2315 22-23 0.7685530735871566 2315 24-25 0.7695575160832604 2315 26-27 0.741354993113589 2315 28-29 0.7203857850634776 2315 30-31 0.7990827469762607 2315 32-33 0.7210031332582574 2315 34-35 0.7923195790142401 2315 36-37 0.8047971456657166 2315 38-39 0.84940259466984 2315 40-41 0.846327271630777 2315 42-43 0.8635400596719904 2315 44-45 0.8786303340485802 2315 46-47 0.8464734150333157 2315 48-49 0.7405667624636152 2315 50-51 0.8114447937528482 2315 52-53 0.8855533919289371 2315 54-55 0.6982226717140421 2315 56-57 0.7215240753765073 2315 58-59 0.6240819170422114 2315 60-61 0.7710538671821041 2315 62-63 0.8601053184871787 2315 64-65 0.9034070142646726 2315 66-67 0.9340902164995128 2315 68-69 0.9615032557705305 2315 70-71 0.8033675879389364 2315 72-73 0.8800574863929072 2315 74-75 0.2797316529409599 2315 76-77 0.3254840190354358 2315 78-79 0.7005382491913181 2315 80-81 0.6958749331981977 2315 82-83 0.6450896077292683 2315 84-85 0.6644441824796026 2315 86-87 0.722345969508364 2315 88-89 0.6739006057888552 2315 90-91 0.7159246888486805 2315 92-93 0.7758666942180916 2315 94-95 0.8299296523487953 2315 96-97 0.909172798185903 2315 98-99 0.9529428739398504 2315 100 0.8828753504259446 2316 1 0.29577079450371 2316 2 0.07149782057216214 2316 3 0.20388593472238625 2316 4 0.0010262746366294095 2316 5 0.27208945632809645 2316 6 0.3273908075058003 2316 7 0.44371521081845344 2316 8 0.4354613659739357 2316 9 0.598258052438652 2316 10-11 0.5999833005843982 2316 12-13 0.39540950250660245 2316 14-15 0.48527715028389906 2316 16-17 0.766617696557347 2316 18-19 0.7300957682874767 2316 20-21 0.7014237211821168 2316 22-23 0.7463664706800586 2316 24-25 0.46143086541395206 2316 26-27 0.7136023482778882 2316 28-29 0.89824879122434 2316 30-31 0.54426424938152 2316 32-33 0.6667744328406116 2316 34-35 0.7849767088393875 2316 36-37 0.843937017202407 2316 38-39 0.7680291068784015 2316 40-41 0.7514751809124505 2316 42-43 0.7167665838890542 2316 44-45 0.561031335157459 2316 46-47 0.7704311357748139 2316 48-49 0.863732027983616 2316 50-51 0.8418783829701084 2316 52-53 0.8762258025396505 2316 54-55 0.711350115640137 2316 56-57 0.8854431005696668 2316 58-59 0.760057550630556 2316 60-61 0.8371893056631947 2316 62-63 0.8786812688121657 2316 64-65 0.8795126568408165 2316 66-67 0.8503456546513348 2316 68-69 0.8995003578715455 2316 70-71 0.8136880500730896 2316 72-73 0.8633974192165965 2316 74-75 0.8672343485340761 2316 76-77 0.7412766610390449 2316 78-79 0.48722004553261655 2316 80-81 0.4597070771648788 2316 82-83 0.3828794972841152 2316 84-85 0.5042787970258225 2316 86-87 0.5548765070862736 2316 88-89 0.534161166934151 2316 90-91 0.39213004433348786 2316 92-93 0.3047861168498649 2316 94-95 0.330966374056441 2316 96-97 0.5187781257603383 2316 98-99 0.7025523653832728 2316 100 0.3141428304600815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 7.0 12 32.0 13 169.0 14 812.0 15 2291.0 16 5744.0 17 12398.0 18 21539.0 19 32615.0 20 48031.0 21 69706.0 22 85829.0 23 85116.0 24 79693.0 25 81674.0 26 93635.0 27 113281.0 28 140136.0 29 173968.0 30 213284.0 31 266892.0 32 340892.0 33 452796.0 34 664284.0 35 932561.0 36 1480795.0 37 2186223.0 38 1799471.0 39 334325.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.199974696416184 21.36595292648094 13.578996337156973 22.855076039945896 2 15.22795504367461 20.186326072479027 41.44321724363032 23.14250164021604 3 18.82146876098451 26.857302850971248 29.135083418465417 25.186144969578827 4 12.15391735313966 16.709137615343728 39.732097455489914 31.404847576026707 5 12.65393746516856 38.84433917609705 34.645190606686484 13.856532752047904 6 29.520863465700348 38.4414572527184 17.90408996204248 14.13358931953878 7 27.065122119022337 32.39244703359963 22.887317349345174 17.655113498032854 8 26.402209784660418 35.590331657033914 19.561942954232826 18.445515604072842 9 26.418718944659474 15.40160679147102 21.77475466261691 36.404919601252594 10-11 25.847733516289168 26.534646231796703 26.859001815409606 20.758618436504523 12-13 25.804873932804632 24.204417882680282 27.891514195486216 22.09919398902887 14-15 23.10859949484587 25.344955075115593 25.89003935081952 25.656406079219018 16-17 21.328894721699605 28.151611141317883 27.142386597358936 23.377107539623577 18-19 20.777549867095164 27.870552997705754 29.93865550853283 21.41324162666625 20-21 22.408903431087406 26.92455550809607 29.405135564281192 21.26140549653533 22-23 22.643110392919205 26.78574662551789 29.04275695414557 21.52838602741733 24-25 21.988895852933645 26.711111586737875 29.996004392184496 21.303988168143988 26-27 21.947034621765795 27.037746837023008 28.927715209182463 22.08750333202873 28-29 21.11852251025986 27.43280580493554 29.562626379973356 21.88604530483125 30-31 22.10188023275162 27.01372473901263 29.474534829649603 21.409860198586152 32-33 22.37559330811278 27.246615543238185 28.633428633809416 21.74436251483962 34-35 22.31340324093482 27.065509016931944 29.396382811174032 21.224704930959202 36-37 21.94163239029369 26.771781963617368 29.039037881454334 22.247547764634607 38-39 22.08002722898178 26.90073339990313 29.60830652736 21.410932843755088 40-41 21.771219136080365 26.060456449165027 30.14777678318495 22.02054763156966 42-43 21.83975451061231 26.25885572363788 29.362587523096433 22.538802242653375 44-45 21.884731892331907 27.147194863480152 29.144126041914838 21.823947202273107 46-47 21.93571480729165 26.981551974132824 28.51282812352778 22.56990509504775 48-49 22.682277989163097 26.961755333248817 28.455626851222238 21.90033982636585 50-51 22.319599962334383 26.793698933373538 28.315693440985644 22.571007663306432 52-53 21.778442240260816 27.37610986374317 28.056434373223983 22.789013522772034 54-55 21.776729167362383 27.298850589498606 28.032415927286035 22.892004315852972 56-57 22.687308304047185 26.819029759557054 27.69335418358297 22.80030775281279 58-59 21.61909982273606 27.336249021796448 28.44778256968239 22.596868585785103 60-61 21.846656856826606 27.93649093284396 27.678726657445434 22.538125552883994 62-63 22.18890295542308 27.210182318541452 27.38900326308607 23.2119114629494 64-65 21.43210252452737 27.356510248710986 27.735008442609633 23.476378784152015 66-67 21.909834850692864 26.99487650160831 27.741972052400214 23.353316595298608 68-69 21.50981105418137 27.096696673445315 27.890149470350206 23.503342802023113 70-71 21.516885903331676 26.70583387362836 28.056345800485584 23.720934422554382 72-73 22.088553293315528 26.85880710865794 28.068138159024453 22.984501439002084 74-75 22.25870071498315 27.189226393865095 27.614252188525136 22.93782070262662 76-77 21.953418124284553 27.093741305424363 27.347938144595187 23.604902425695894 78-79 21.80820873426253 27.155137768495248 27.425996215381375 23.610657281860846 80-81 21.37153915132852 27.680023658900637 27.796105799697752 23.152331390073087 82-83 22.28591907707478 26.674288671892953 28.749096767675137 22.29069548335713 84-85 22.471922779168313 27.09274922466652 27.467470792087184 22.967857204077983 86-87 21.20841152306423 27.771242089821286 27.89495912786901 23.12538725924547 88-89 22.100927789398536 26.739771809352796 27.734163966249863 23.4251364349988 90-91 21.82041210691132 26.76730107524391 27.863181473179644 23.54910534466513 92-93 22.14268684962101 26.974118457504453 27.67031117721273 23.2128835156618 94-95 21.057343917056954 26.926860563924855 28.278876678474575 23.736918840543616 96-97 21.393096695705058 26.945355636889 27.79464044764896 23.86690721975698 98-99 20.738304587861496 27.51377887862658 27.659881152379562 24.08803538113236 100 21.358434920519898 26.96791892568462 27.084407258941102 24.58923889485438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1188.0 1 837.5 2 728.0 3 1017.5 4 881.0 5 746.5 6 1173.0 7 2349.5 8 3806.0 9 4689.5 10 4582.0 11 4435.0 12 5006.0 13 6047.0 14 7349.0 15 8890.0 16 10473.5 17 12017.5 18 13704.5 19 15327.5 20 16893.0 21 18506.0 22 20494.0 23 23164.0 24 26207.5 25 29939.5 26 35714.5 27 42103.0 28 50529.5 29 67956.0 30 93798.0 31 118071.5 32 152119.5 33 191339.0 34 221490.0 35 251449.5 36 279211.0 37 320532.0 38 337964.5 39 320603.5 40 296721.0 41 271375.0 42 263789.5 43 273746.0 44 297476.5 45 347403.5 46 390246.0 47 416345.5 48 437871.5 49 431912.0 50 411564.0 51 414800.0 52 414330.0 53 388237.0 54 363449.0 55 313214.5 56 268303.0 57 218811.0 58 170062.0 59 163423.0 60 134640.5 61 89560.0 62 65617.5 63 55290.5 64 39878.0 65 18708.0 66 10184.5 67 6906.0 68 5225.5 69 4220.0 70 3296.5 71 2381.0 72 1750.5 73 1487.5 74 1158.5 75 766.0 76 510.0 77 291.5 78 183.0 79 122.5 80 76.0 81 55.0 82 37.5 83 20.5 84 13.5 85 8.5 86 6.5 87 4.5 88 4.0 89 4.0 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04314570798474455 2 0.0 3 0.0 4 0.03453303028781367 5 0.0 6 0.0 7 9.260943760140734E-4 8 0.0 9 0.011349801074928032 10-11 4.0645253169506554E-4 12-13 2.0579875022534963E-5 14-15 1.0289937511267483E-4 16-17 2.881182503154895E-4 18-19 0.0 20-21 1.0289937511267482E-5 22-23 0.0 24-25 6.276861881873164E-4 26-27 5.505116568528103E-4 28-29 1.9550881271408212E-4 30-31 0.0023975554401253233 32-33 0.014627146172266724 34-35 0.02526179659016167 36-37 0.03049422981464118 38-39 0.021752927898819455 40-41 0.001327401938953505 42-43 1.1833428137957603E-4 44-45 0.0118025583254238 46-47 0.018794570864330055 48-49 0.003375099503695734 50-51 0.0118025583254238 52-53 0.011365235981194934 54-55 0.010634650417894942 56-57 4.4246731298450175E-4 58-59 0.008247384915280888 60-61 0.004512137598690791 62-63 0.00134798181397604 64-65 0.0362463048834397 66-67 0.06195056878658587 68-69 0.06041222312865138 70-71 0.05324528165205358 72-73 0.04688095530133465 74-75 0.036297754570996044 76-77 0.03213547484768834 78-79 0.01577447420477305 80-81 0.0011679079075288593 82-83 0.03926125657424107 84-85 0.05302919296431696 86-87 0.05912598093974295 88-89 0.07123209242174913 90-91 0.04995250164844799 92-93 0.04008959654389811 94-95 0.013366628827136457 96-97 0.0035963331601879846 98-99 0.021536839211082837 100 0.03185764653488412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9718232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.55796877702933 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61294605196352 46.676343494809075 2 15.435307075907104 19.312029162369132 3 5.08576018881528 9.544644812980954 4 2.1399247573259714 5.354773846159608 5 1.0257247843266248 3.2083629515865066 6 0.5606061957791431 2.1042230935056496 7 0.3250438532453275 1.4233858255740548 8 0.20118971999844035 1.0068816177537365 9 0.12751478077228706 0.7179359106746225 >10 0.4395655740629425 4.547039107114048 >50 0.02470526804673903 1.0806829332437144 >100 0.019123656279765446 2.3321382951868723 >500 0.0017834706326765488 0.7598294584866553 >1k 7.176365951425096E-4 0.712234713381044 >5k 2.1746562280169817E-5 0.1072555730440555 >10k+ 6.523968684050945E-5 1.1122392041302442 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34653 0.35657720457795206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29399 0.3025138728937527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17705 0.18218334363699076 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0289937511267482E-5 0.0 12-13 0.0 0.0 0.0 4.115975004506993E-5 0.0 14-15 0.0 5.144968755633741E-6 0.0 4.630471880070367E-5 0.0 16-17 0.0 1.0289937511267482E-5 0.0 7.202956257887237E-5 0.0 18-19 0.0 1.0289937511267482E-5 0.0 1.234792501352098E-4 0.0 20-21 0.0 2.0579875022534963E-5 0.0 1.9550881271408215E-4 0.0 22-23 0.0 2.0579875022534963E-5 0.0 4.013075629394318E-4 0.0 24-25 0.0 2.0579875022534963E-5 0.0 9.003695322359047E-4 0.0 26-27 0.0 2.0579875022534963E-5 0.0 0.0018779135958063155 0.0 28-29 0.0 2.0579875022534963E-5 0.0 0.005314752724569654 0.0 30-31 0.0 2.0579875022534963E-5 0.0 0.018984934708288503 5.144968755633741E-6 32-33 0.0 2.0579875022534963E-5 0.0 0.05264846527640007 2.0579875022534963E-5 34-35 0.0 2.0579875022534963E-5 0.0 0.10348075658206143 2.0579875022534963E-5 36-37 0.0 2.0579875022534963E-5 0.0 0.189231950832209 2.0579875022534963E-5 38-39 0.0 2.5724843778168707E-5 0.0 0.31037024018360543 2.0579875022534963E-5 40-41 0.0 3.086981253380245E-5 0.0 0.43867547101159965 2.0579875022534963E-5 42-43 0.0 3.086981253380245E-5 0.0 0.56256631864726 2.0579875022534963E-5 44-45 0.0 3.086981253380245E-5 0.0 0.7097227149958965 2.0579875022534963E-5 46-47 0.0 3.086981253380245E-5 0.0 0.8832573661546668 2.0579875022534963E-5 48-49 0.0 3.086981253380245E-5 0.0 1.0762811589597778 2.0579875022534963E-5 50-51 0.0 3.086981253380245E-5 0.0 1.2755921035842732 2.0579875022534963E-5 52-53 0.0 3.086981253380245E-5 0.0 1.4628432414455634 2.5724843778168707E-5 54-55 0.0 5.144968755633741E-5 0.0 1.6648244248542325 3.086981253380245E-5 56-57 0.0 5.144968755633741E-5 0.0 1.8990542724232142 3.086981253380245E-5 58-59 0.0 5.144968755633741E-5 0.0 2.170873261720856 3.086981253380245E-5 60-61 1.0289937511267482E-5 5.144968755633741E-5 0.0 2.4438910287385607 3.086981253380245E-5 62-63 1.0289937511267482E-5 5.6594656311971154E-5 0.0 2.717474742319385 3.086981253380245E-5 64-65 1.0289937511267482E-5 6.17396250676049E-5 0.0 2.9929312245272595 3.086981253380245E-5 66-67 1.0289937511267482E-5 8.231950009013985E-5 0.0 3.300476876864022 3.086981253380245E-5 68-69 1.0289937511267482E-5 8.231950009013985E-5 0.0 3.639231909672459 3.086981253380245E-5 70-71 1.0289937511267482E-5 8.231950009013985E-5 0.0 4.003629466758975 3.086981253380245E-5 72-73 1.0289937511267482E-5 8.231950009013985E-5 0.0 4.367599991438772 3.086981253380245E-5 74-75 1.0289937511267482E-5 8.231950009013985E-5 0.0 4.715276400069477 3.086981253380245E-5 76-77 1.0289937511267482E-5 8.231950009013985E-5 0.0 5.10534220627785 3.086981253380245E-5 78-79 1.0289937511267482E-5 8.231950009013985E-5 0.0 5.523628166110873 3.086981253380245E-5 80-81 1.0289937511267482E-5 8.231950009013985E-5 0.0 5.974353153948167 3.086981253380245E-5 82-83 1.0289937511267482E-5 8.231950009013985E-5 0.0 6.428751649476983 3.086981253380245E-5 84-85 1.0289937511267482E-5 8.231950009013985E-5 0.0 6.8625033853894415 3.086981253380245E-5 86-87 1.0289937511267482E-5 8.231950009013985E-5 0.0 7.3174523925751105 3.086981253380245E-5 88 1.0289937511267482E-5 8.231950009013985E-5 0.0 7.68277604403764 3.086981253380245E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21390 0.0 48.228603 1 GTATCAA 37455 0.0 38.916225 1 GGGCACC 9795 0.0 36.620388 94 TACCTGG 5185 0.0 36.167225 2 GTACTAG 9330 0.0 35.781254 1 ATCAACG 42785 0.0 32.4415 3 GTGGTAT 6600 0.0 32.415024 1 CAACGCA 42835 0.0 32.381496 5 ACCTGGG 5720 0.0 31.554714 3 CTGTGCG 12600 0.0 31.174974 9 TATCAAC 46990 0.0 30.74606 2 TAGGCAT 11640 0.0 30.477076 5 TAGTACT 13475 0.0 29.75427 4 AACGCAG 48590 0.0 29.300499 6 ACTGTGC 14565 0.0 28.388506 8 TCAACGC 50255 0.0 27.956041 4 GTACTGT 14935 0.0 27.276785 6 CTAGGCA 13380 0.0 27.190294 4 GCATCGT 11165 0.0 27.143845 8 ACGCAGA 51695 0.0 26.995241 7 >>END_MODULE