FastQCFastQC Report
Mon 6 Jun 2016
ERR1211237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10345755
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261020.2522967149328396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215010.20782436854535993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125260.12107381239938506No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161750.045.8535921
GTATCAA289400.040.198641
CAACGCA319000.034.2755625
GTACTAG72000.033.988521
ATCAACG324450.033.801393
GTGGTAT58400.032.8782541
TATCAAC359150.032.102672
AACGCAG363100.030.8638236
TCAACGC375600.029.5358734
GCATCGT81750.027.7148278
ACGCAGA395950.027.579277
CTGTGCG110350.027.517769
GTACATG426150.027.4978221
TAGTACT111700.027.312094
TAGGCAT96350.027.02865
TACATGG428750.026.935272
CGCAGAG405500.026.9168538
TACCTGG65000.026.281142
ACATGGG428000.025.8212013
CTAGTAC120750.025.7714273
Produced by FastQC (version 0.11.5)