##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211237_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10345755 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45640187690507 31.0 31.0 34.0 29.0 34.0 2 31.47379616084085 31.0 31.0 34.0 28.0 34.0 3 31.54264101556629 33.0 31.0 34.0 28.0 34.0 4 35.13736696838462 37.0 35.0 37.0 32.0 37.0 5 35.191758745495136 37.0 35.0 37.0 32.0 37.0 6 35.25173252217939 37.0 35.0 37.0 32.0 37.0 7 35.16213703108183 37.0 35.0 37.0 32.0 37.0 8 35.16585014820088 37.0 35.0 37.0 32.0 37.0 9 36.73221558020657 39.0 37.0 39.0 32.0 39.0 10-11 36.68555494499918 39.0 37.0 39.0 32.0 39.0 12-13 36.57589194795353 39.0 37.0 39.0 32.0 39.0 14-15 37.781357861267736 40.0 37.0 41.0 32.0 41.0 16-17 37.75777761023724 40.0 37.0 41.0 32.0 41.0 18-19 37.75827114599176 40.0 37.0 41.0 32.0 41.0 20-21 37.71986771385946 40.0 37.0 41.0 32.0 41.0 22-23 37.61166478425209 40.0 37.0 41.0 32.0 41.0 24-25 37.520611497179274 40.0 37.0 41.0 31.5 41.0 26-27 37.375802684289354 40.0 37.0 41.0 31.0 41.0 28-29 37.255434136996286 40.0 36.0 41.0 31.0 41.0 30-31 37.110929120204375 39.5 36.0 41.0 30.5 41.0 32-33 36.93934546101275 39.0 36.0 41.0 30.0 41.0 34-35 36.796268276215706 39.0 35.5 41.0 30.0 41.0 36-37 36.696804679793786 39.0 35.0 41.0 30.0 41.0 38-39 36.55688511858246 39.0 35.0 41.0 30.0 41.0 40-41 36.38973743337243 39.0 35.0 40.5 29.0 41.0 42-43 36.175104765191136 39.0 35.0 40.0 28.5 41.0 44-45 35.95117630371104 38.0 35.0 40.0 28.0 41.0 46-47 35.71985137865724 38.0 34.5 40.0 27.0 41.0 48-49 35.51770798747892 38.0 34.0 40.0 26.5 41.0 50-51 34.82153105307442 37.5 33.5 39.5 25.5 40.5 52-53 34.91648710026479 38.0 33.5 39.5 26.0 40.5 54-55 35.394243001114944 38.0 34.0 40.0 27.0 41.0 56-57 35.34343230629374 38.0 34.0 40.0 26.5 41.0 58-59 35.115064149499 37.5 34.0 40.0 26.0 41.0 60-61 34.88154532946122 37.0 34.0 40.0 26.0 41.0 62-63 34.578360158345134 36.5 33.0 40.0 26.0 41.0 64-65 34.31739786028183 36.0 33.0 39.0 26.0 41.0 66-67 34.14351407896282 35.5 33.0 39.0 26.0 41.0 68-69 33.851222361248645 35.0 33.0 39.0 25.5 41.0 70-71 33.5590611801652 35.0 33.0 38.5 25.0 40.5 72-73 33.23412776544583 35.0 32.5 37.5 25.0 40.0 74-75 32.91606233667818 35.0 32.0 37.0 24.0 39.5 76-77 32.6043983256901 35.0 32.0 36.5 24.0 39.0 78-79 32.286257890313465 35.0 31.5 36.0 23.0 39.0 80-81 32.01119802276393 35.0 31.0 35.5 23.0 37.5 82-83 31.736806496964213 35.0 31.0 35.0 22.5 37.0 84-85 31.508551720004967 34.5 31.0 35.0 21.5 37.0 86-87 31.295920742372115 34.0 31.0 35.0 20.0 36.0 88-89 31.09867612368551 34.0 31.0 35.0 20.0 36.0 90-91 30.886462370315165 34.0 30.0 35.0 20.0 36.0 92-93 30.696359183065905 34.0 30.0 35.0 19.0 35.0 94-95 30.536883533391233 34.0 30.0 35.0 19.0 35.0 96-97 30.359475891319676 34.0 29.5 35.0 18.5 35.0 98-99 30.130483807126694 34.0 29.0 35.0 18.0 35.0 100 28.970309271773786 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4873983492340592 1101 2 -0.39274569131922377 1101 3 -0.43695371039805053 1101 4 -0.4219429205756242 1101 5 -0.356263115951883 1101 6 -0.32299762888773387 1101 7 -0.3403585836505556 1101 8 -0.2643803683884869 1101 9 -0.4991999234351212 1101 10-11 -0.4565260000475888 1101 12-13 -0.4902493387597602 1101 14-15 -0.6637864952333743 1101 16-17 -0.6389805886730215 1101 18-19 -0.7115966056448073 1101 20-21 -0.6832679859278343 1101 22-23 -0.7888169718968783 1101 24-25 -0.9063913315646417 1101 26-27 -0.8102029863032598 1101 28-29 -0.7807662813597034 1101 30-31 -0.8627780570769872 1101 32-33 -0.9268214382259714 1101 34-35 -0.9243867733587479 1101 36-37 -0.9959006699722721 1101 38-39 -1.0009750501701475 1101 40-41 -0.9950750365142227 1101 42-43 -1.0709776919634848 1101 44-45 -1.0954843755011225 1101 46-47 -1.2702871484429963 1101 48-49 -1.23661903396912 1101 50-51 -1.2677777096297191 1101 52-53 -1.0779087369934786 1101 54-55 -0.9421061481329573 1101 56-57 -0.9308334187997644 1101 58-59 -0.9046480632548679 1101 60-61 -0.9317120851138441 1101 62-63 -0.9672766099624255 1101 64-65 -0.8914470605797788 1101 66-67 -0.9407672612148374 1101 68-69 -0.8933333649129835 1101 70-71 -0.946710545615197 1101 72-73 -0.9824495458276985 1101 74-75 -1.035401119575166 1101 76-77 -1.0755298379524802 1101 78-79 -1.0690977226480172 1101 80-81 -1.1481821094844946 1101 82-83 -1.16455060681335 1101 84-85 -1.3207465862175631 1101 86-87 -1.282952544722125 1101 88-89 -1.3552689967084035 1101 90-91 -1.495337072065805 1101 92-93 -1.5912947771737258 1101 94-95 -1.6905212007313182 1101 96-97 -1.607102337196654 1101 98-99 -1.7557477094014828 1101 100 -1.796011986353836 1102 1 -0.46658473049092564 1102 2 -0.47848531321355736 1102 3 -0.5234008178021909 1102 4 -0.2987384772625603 1102 5 -0.32490225743839574 1102 6 -0.3322847675558904 1102 7 -0.356888998876137 1102 8 -0.3872877976469482 1102 9 -0.5406341589105139 1102 10-11 -0.5792941866467558 1102 12-13 -0.6100825136222525 1102 14-15 -0.7545145268264335 1102 16-17 -0.7700821987228963 1102 18-19 -0.7573865928968573 1102 20-21 -0.7747781552854605 1102 22-23 -0.7969028874060271 1102 24-25 -0.798655622740263 1102 26-27 -0.8536682167116041 1102 28-29 -0.9034982419044866 1102 30-31 -0.9140364195548116 1102 32-33 -0.9653494391957196 1102 34-35 -0.9638685630726584 1102 36-37 -0.9003844306550235 1102 38-39 -1.0618243807330217 1102 40-41 -1.107857982077391 1102 42-43 -1.0527470502846228 1102 44-45 -1.1913419485235721 1102 46-47 -1.223076737881506 1102 48-49 -1.1787611044890838 1102 50-51 -1.124439339979304 1102 52-53 -1.1054243850653194 1102 54-55 -0.9082837447780676 1102 56-57 -0.9010565013877923 1102 58-59 -0.895686263400151 1102 60-61 -0.8682757391724252 1102 62-63 -0.9482066832819172 1102 64-65 -1.011573267741639 1102 66-67 -1.0834460112568038 1102 68-69 -1.0715226354762422 1102 70-71 -1.0577824902311548 1102 72-73 -1.0778971224907323 1102 74-75 -1.1638286925333645 1102 76-77 -1.0922017808218953 1102 78-79 -1.1073087611956574 1102 80-81 -1.2424878443070142 1102 82-83 -1.2131189938667433 1102 84-85 -1.2664320003603784 1102 86-87 -1.277565150705815 1102 88-89 -1.2713386018169288 1102 90-91 -1.4043855418365467 1102 92-93 -1.4644714895976954 1102 94-95 -1.5152733613538736 1102 96-97 -1.5418327719147378 1102 98-99 -1.6122504202075945 1102 100 -1.6451681890733205 1103 1 -0.5539889529623139 1103 2 -0.4955782312489312 1103 3 -0.5461757151139857 1103 4 -0.4370962773403164 1103 5 -0.41767082160655633 1103 6 -0.44033263800817224 1103 7 -0.4465359204837611 1103 8 -0.44634971483151986 1103 9 -0.5870781100258569 1103 10-11 -0.5976073053819277 1103 12-13 -0.6652779902701909 1103 14-15 -0.8251205478204682 1103 16-17 -0.8474839476622122 1103 18-19 -0.7263984252826603 1103 20-21 -0.8004051928504694 1103 22-23 -0.8208673789796777 1103 24-25 -0.88821626371751 1103 26-27 -0.8955839757591448 1103 28-29 -0.9346372160929803 1103 30-31 -0.9966698353392118 1103 32-33 -1.0749005635540883 1103 34-35 -0.9888088715035153 1103 36-37 -0.9469240494268263 1103 38-39 -0.9849667750444624 1103 40-41 -1.1215151753227843 1103 42-43 -1.1247761748571108 1103 44-45 -1.1577055126635543 1103 46-47 -1.132648918252137 1103 48-49 -1.185700962162386 1103 50-51 -1.271996155151811 1103 52-53 -1.1458694968813674 1103 54-55 -0.8768807844278328 1103 56-57 -0.885791709496921 1103 58-59 -0.8948381686959834 1103 60-61 -0.9216638357923088 1103 62-63 -0.8650046875528474 1103 64-65 -0.8866853486075428 1103 66-67 -1.0614164359267662 1103 68-69 -1.0106463515215225 1103 70-71 -0.9444646498105982 1103 72-73 -0.9577629995804173 1103 74-75 -0.9669949488228404 1103 76-77 -0.9583070101729732 1103 78-79 -1.015766139251653 1103 80-81 -1.0445481725712042 1103 82-83 -0.9346260172328869 1103 84-85 -1.0000437121685337 1103 86-87 -1.1463232347608319 1103 88-89 -1.1950865212890882 1103 90-91 -1.2964331925445158 1103 92-93 -1.3883149978437395 1103 94-95 -1.3493026662293808 1103 96-97 -1.4438512360710618 1103 98-99 -1.4693713246526308 1103 100 -1.4378383866741622 1104 1 -0.4389806496223798 1104 2 -0.45156818847414826 1104 3 -0.49547188945130216 1104 4 -0.34110152846438524 1104 5 -0.3110050447136885 1104 6 -0.406977191607929 1104 7 -0.38624354227155067 1104 8 -0.38626536865116634 1104 9 -0.47858500759611644 1104 10-11 -0.5400153396017586 1104 12-13 -0.5321729568489815 1104 14-15 -0.7412808165029716 1104 16-17 -0.7712946774590534 1104 18-19 -0.8020592354873486 1104 20-21 -0.7672478341658788 1104 22-23 -0.7908656942440402 1104 24-25 -0.815939540418924 1104 26-27 -0.8477352190721703 1104 28-29 -0.8531474940743635 1104 30-31 -0.8509837869194712 1104 32-33 -0.9037948299829992 1104 34-35 -0.992193100627567 1104 36-37 -0.8916433330859803 1104 38-39 -0.9878355407021076 1104 40-41 -0.9720942758489883 1104 42-43 -1.0171298052436129 1104 44-45 -1.0538030632329765 1104 46-47 -1.0836402757771069 1104 48-49 -1.0588030746458372 1104 50-51 -1.2083880202854402 1104 52-53 -0.9465289724253552 1104 54-55 -0.8212307499673344 1104 56-57 -0.8108924107407844 1104 58-59 -0.8174934761602373 1104 60-61 -0.8507245847338822 1104 62-63 -0.9628101224662586 1104 64-65 -0.9592421318564561 1104 66-67 -0.9108659102673684 1104 68-69 -1.0367165634262605 1104 70-71 -0.9838243080499396 1104 72-73 -0.9028648385678295 1104 74-75 -0.8938197253765736 1104 76-77 -0.8951317210876546 1104 78-79 -0.9396325309636282 1104 80-81 -0.9957492031043031 1104 82-83 -0.9914120558067694 1104 84-85 -0.9531601120853921 1104 86-87 -0.9691849223408262 1104 88-89 -1.0268148303177398 1104 90-91 -1.0971560425921076 1104 92-93 -1.1299398706680819 1104 94-95 -1.0845313964419425 1104 96-97 -1.2457057033554655 1104 98-99 -1.2889587722781357 1104 100 -1.2344153758109009 1105 1 -0.5946417198998795 1105 2 -0.5507380581208885 1105 3 -0.5332930786774099 1105 4 -0.33837131186222535 1105 5 -0.28533890917484683 1105 6 -0.4021776414740046 1105 7 -0.3462337760321006 1105 8 -0.42539778351510193 1105 9 -0.6146257830038024 1105 10-11 -0.546767798130972 1105 12-13 -0.546231864637754 1105 14-15 -0.7538347064411184 1105 16-17 -0.7356697874041487 1105 18-19 -0.7463497780501456 1105 20-21 -0.7271925379112432 1105 22-23 -0.7417526570925119 1105 24-25 -0.7701433829258804 1105 26-27 -0.7530756877727711 1105 28-29 -0.7743872283734987 1105 30-31 -0.8175854272329772 1105 32-33 -0.8567534920487105 1105 34-35 -0.8862525421236 1105 36-37 -0.9517242863044117 1105 38-39 -0.9068291186042856 1105 40-41 -0.9280429811486641 1105 42-43 -0.978467639077131 1105 44-45 -1.0154523538409563 1105 46-47 -1.0523921571437214 1105 48-49 -1.0096893638936635 1105 50-51 -1.020492593801059 1105 52-53 -0.9205299596113576 1105 54-55 -0.7387397713122326 1105 56-57 -0.6382581290977285 1105 58-59 -0.6287910060418653 1105 60-61 -0.5703977524048511 1105 62-63 -0.6286950710871295 1105 64-65 -0.6639222261898681 1105 66-67 -0.7166896965958074 1105 68-69 -0.7407867096760654 1105 70-71 -0.6868627405991177 1105 72-73 -0.6832919188400908 1105 74-75 -0.7190484591412059 1105 76-77 -0.721436141203391 1105 78-79 -0.7761510325748731 1105 80-81 -0.7631474564804464 1105 82-83 -0.8596769950351657 1105 84-85 -0.8319486172810535 1105 86-87 -0.9659547755021372 1105 88-89 -0.9326501152787294 1105 90-91 -0.9836473975582471 1105 92-93 -0.8973152216221045 1105 94-95 -0.9796813920026466 1105 96-97 -0.9596810717304187 1105 98-99 -0.9585731836504294 1105 100 -1.1053232707187668 1106 1 -0.5676828608048261 1106 2 -0.5473078278537677 1106 3 -0.6089734018312996 1106 4 -0.42624640982632656 1106 5 -0.42391466183172355 1106 6 -0.459702026593483 1106 7 -0.4204287882286408 1106 8 -0.43398893508813075 1106 9 -0.5149564427911315 1106 10-11 -0.5053183429507868 1106 12-13 -0.5977132480406979 1106 14-15 -0.8040189678835716 1106 16-17 -0.8069267917685039 1106 18-19 -0.890442964770088 1106 20-21 -0.7957931734558343 1106 22-23 -0.7737143658849917 1106 24-25 -0.8055512499191764 1106 26-27 -0.8590654552045791 1106 28-29 -0.9117028653843704 1106 30-31 -0.921762773450105 1106 32-33 -0.9540412406070757 1106 34-35 -0.9534327211841216 1106 36-37 -1.0189707658627896 1106 38-39 -1.0125873812916453 1106 40-41 -1.0467919116591347 1106 42-43 -1.065939204113974 1106 44-45 -1.0386959912623936 1106 46-47 -0.9922948122853157 1106 48-49 -1.0353117171677155 1106 50-51 -1.0058828658705323 1106 52-53 -0.9700500516734039 1106 54-55 -0.8941420292465807 1106 56-57 -0.8552595665219798 1106 58-59 -0.8790743456225272 1106 60-61 -0.8796282569768792 1106 62-63 -0.9320563406044258 1106 64-65 -0.9208467396556657 1106 66-67 -0.8887234956169365 1106 68-69 -0.9622876827585358 1106 70-71 -0.9088012742210125 1106 72-73 -0.8520197915498997 1106 74-75 -0.8679700898297362 1106 76-77 -0.8565024517746807 1106 78-79 -0.8669689692847484 1106 80-81 -0.8385922942604296 1106 82-83 -0.8500118208897192 1106 84-85 -0.8599311155527012 1106 86-87 -0.9397207917408075 1106 88-89 -0.9523644670412921 1106 90-91 -1.0134400532308057 1106 92-93 -1.0715628951093628 1106 94-95 -1.056018123663442 1106 96-97 -1.1071708077179565 1106 98-99 -1.087774488924289 1106 100 -1.309922891858161 1107 1 -0.659912066564722 1107 2 -0.6080536426483754 1107 3 -0.6170429970696283 1107 4 -0.5653665665120116 1107 5 -0.5122353262848023 1107 6 -0.5879094237413796 1107 7 -0.5274372164052679 1107 8 -0.5503598660400186 1107 9 -0.6605931831929723 1107 10-11 -0.6681086352114392 1107 12-13 -0.7552061900731033 1107 14-15 -0.9233818937562717 1107 16-17 -0.8929805939487494 1107 18-19 -0.9612893686668968 1107 20-21 -0.8672171305869014 1107 22-23 -0.9642444253806559 1107 24-25 -0.9053771995783961 1107 26-27 -0.9513851159157625 1107 28-29 -1.0247592001350228 1107 30-31 -1.1055743737135302 1107 32-33 -1.0630323597246019 1107 34-35 -0.9896862728789131 1107 36-37 -1.1075469500445791 1107 38-39 -1.0707747603792441 1107 40-41 -1.1250781117558688 1107 42-43 -1.0923303587868318 1107 44-45 -1.0796615488179668 1107 46-47 -0.967727203354869 1107 48-49 -0.9086008063974944 1107 50-51 -0.9859044551952536 1107 52-53 -0.928838184959524 1107 54-55 -0.920099098988139 1107 56-57 -0.8684208513065457 1107 58-59 -0.9112634492317397 1107 60-61 -0.8531702836110711 1107 62-63 -0.8231220410331872 1107 64-65 -0.8354887245731248 1107 66-67 -0.8454788160566764 1107 68-69 -0.7811834119095593 1107 70-71 -0.8154136394580647 1107 72-73 -0.8257698235660982 1107 74-75 -0.8036645683114685 1107 76-77 -0.7889748918764283 1107 78-79 -0.7703075970838711 1107 80-81 -0.8545705836421646 1107 82-83 -0.8598666580941483 1107 84-85 -0.8942698043771138 1107 86-87 -0.8738137034330116 1107 88-89 -0.841503833444051 1107 90-91 -0.9310074351774738 1107 92-93 -0.9599285525680834 1107 94-95 -1.096853671283796 1107 96-97 -1.0626389719700988 1107 98-99 -1.1016876969201235 1107 100 -1.1114630674621004 1108 1 -0.5500693758832718 1108 2 -0.5346793968292793 1108 3 -0.5423593630195498 1108 4 -0.472950590412232 1108 5 -0.3974330771849921 1108 6 -0.4277568540545431 1108 7 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60-61 1.1786300201717452 2311 62-63 1.1653831552687919 2311 64-65 1.1928641919384972 2311 66-67 1.2122986672403258 2311 68-69 1.1902284948112438 2311 70-71 1.1860547056272281 2311 72-73 1.16270835861679 2311 74-75 1.1828682674433324 2311 76-77 1.1431190217190519 2311 78-79 1.1604477389996788 2311 80-81 1.1174282754384137 2311 82-83 1.21396285327981 2311 84-85 1.2487778304293435 2311 86-87 1.2533192490322485 2311 88-89 1.2450370050532342 2311 90-91 1.317870683849545 2311 92-93 1.3553520727061006 2311 94-95 1.400442683955017 2311 96-97 1.4681598810344205 2311 98-99 1.517853476132732 2311 100 1.621800011118335 2312 1 0.680200319554249 2312 2 0.68170989767329 2312 3 0.6733152870070214 2312 4 0.3778974471037344 2312 5 0.4901581858934989 2312 6 0.5704244957901778 2312 7 0.4440079318766763 2312 8 0.5535573544619936 2312 9 0.7605199482690281 2312 10-11 0.70116028038764 2312 12-13 0.862625842848125 2312 14-15 1.0845884588049515 2312 16-17 1.079046019757385 2312 18-19 1.0562678550838172 2312 20-21 1.0940199924642897 2312 22-23 1.1195309766934756 2312 24-25 1.1234993683206227 2312 26-27 1.1580055329107424 2312 28-29 1.1632150568827697 2312 30-31 1.2108169247683733 2312 32-33 1.2430393922764864 2312 34-35 1.2269737581074835 2312 36-37 1.2076456267369977 2312 38-39 1.255131250451349 2312 40-41 1.379679913562697 2312 42-43 1.4200806899888434 2312 44-45 1.4370915266734698 2312 46-47 1.4538760637083712 2312 48-49 1.4754809633618535 2312 50-51 1.4617163063092846 2312 52-53 1.2703150088366044 2312 54-55 1.2819611459069407 2312 56-57 1.198564007381485 2312 58-59 1.1767794159802847 2312 60-61 1.1360993111693958 2312 62-63 1.1296930361914903 2312 64-65 1.1865059793175874 2312 66-67 1.158974769548486 2312 68-69 1.1698803141247325 2312 70-71 1.128019136514375 2312 72-73 1.1611387125311836 2312 74-75 1.136225657741349 2312 76-77 1.1328772550184638 2312 78-79 1.1393885411760465 2312 80-81 1.1583765207574643 2312 82-83 1.1568421785994474 2312 84-85 1.2348997859619182 2312 86-87 1.24843179989686 2312 88-89 1.1791211405743383 2312 90-91 1.233065746952125 2312 92-93 1.3084377627448909 2312 94-95 1.344558418763949 2312 96-97 1.3577525292625054 2312 98-99 1.0100799905901923 2312 100 1.2999675302785825 2313 1 0.6104408885006869 2313 2 0.6328998811805029 2313 3 0.6349209282284747 2313 4 0.5249835658128461 2313 5 0.37839259319248697 2313 6 0.5141752212353126 2313 7 0.6060201759229571 2313 8 0.607469201437965 2313 9 0.7351771379206014 2313 10-11 0.7474519516237805 2313 12-13 0.8337569622363148 2313 14-15 1.0180511048178786 2313 16-17 1.0296898514791906 2313 18-19 0.9858519212001156 2313 20-21 0.9923502579151702 2313 22-23 0.9986197589035655 2313 24-25 1.0022027607221986 2313 26-27 1.0545760485791575 2313 28-29 1.0631834241395808 2313 30-31 1.0821661346435505 2313 32-33 1.1893406560652835 2313 34-35 1.2517583292536685 2313 36-37 1.2294647549540656 2313 38-39 1.268549704014454 2313 40-41 1.2689955909957078 2313 42-43 1.2748706324308614 2313 44-45 1.2680450197368103 2313 46-47 1.3616864094698613 2313 48-49 1.3665201783575114 2313 50-51 1.3893328851754063 2313 52-53 1.3287613214940563 2313 54-55 1.205996814193 2313 56-57 1.1350463562841 2313 58-59 1.121453688113661 2313 60-61 1.074035788992191 2313 62-63 1.0457138332192173 2313 64-65 1.1417295011372417 2313 66-67 1.1172901185132815 2313 68-69 1.1184451368332802 2313 70-71 1.148605728447187 2313 72-73 1.148907030504425 2313 74-75 1.064236265049992 2313 76-77 0.8880969211389314 2313 78-79 1.0338578508778156 2313 80-81 1.1284516922160037 2313 82-83 1.1459524399891663 2313 84-85 1.1628233398545937 2313 86-87 1.1700854966051573 2313 88-89 1.2504870006660909 2313 90-91 1.2626552197192815 2313 92-93 1.355199655842494 2313 94-95 1.3830837212228708 2313 96-97 1.4359587899574002 2313 98-99 1.4756007708076027 2313 100 1.5572813780555812 2314 1 0.5934028356029124 2314 2 0.5943069331564068 2314 3 0.6281476280048821 2314 4 0.49705333193939083 2314 5 0.4983924002165807 2314 6 0.5349039896004868 2314 7 0.5430112982102457 2314 8 0.5219615660076258 2314 9 0.6971391624184804 2314 10-11 0.6828338678724464 2314 12-13 0.7563607301524016 2314 14-15 0.93251193117149 2314 16-17 0.942000209029672 2314 18-19 0.8889425149695143 2314 20-21 0.8792407363404138 2314 22-23 0.929910175422556 2314 24-25 0.9250454389649576 2314 26-27 0.9671748627627608 2314 28-29 0.9888954000085661 2314 30-31 1.0237417626184495 2314 32-33 0.9787517500651859 2314 34-35 1.047359773298517 2314 36-37 1.0813052600623365 2314 38-39 1.0893033308579803 2314 40-41 1.1603153218887599 2314 42-43 1.251241329748261 2314 44-45 1.2649886740578467 2314 46-47 1.2815208705317929 2314 48-49 1.241652259071941 2314 50-51 1.216405651666662 2314 52-53 1.1704197623062385 2314 54-55 1.0557005602555947 2314 56-57 1.0142603585027103 2314 58-59 1.0578088624186393 2314 60-61 1.021666850680944 2314 62-63 1.0422592694468946 2314 64-65 1.0795045944457087 2314 66-67 1.1100117834149401 2314 68-69 1.1471417763016305 2314 70-71 1.1104961183395972 2314 72-73 1.1135666438505112 2314 74-75 1.080783858912497 2314 76-77 1.1125671265833361 2314 78-79 1.0677970194842459 2314 80-81 1.0501697854211045 2314 82-83 1.091633540483528 2314 84-85 1.2235233327411699 2314 86-87 1.2549921930127006 2314 88-89 1.2869167003658148 2314 90-91 1.3644419956657394 2314 92-93 1.3584994667979657 2314 94-95 1.4084036416531553 2314 96-97 1.4057491693695816 2314 98-99 1.5037366638739016 2314 100 1.5923398389762653 2315 1 0.45354296845664166 2315 2 0.4763030793438361 2315 3 0.543844287952929 2315 4 0.25428427504420625 2315 5 0.3289655962428597 2315 6 0.41258283455150746 2315 7 0.426879662825435 2315 8 0.4506617520032634 2315 9 0.6567268004374185 2315 10-11 0.49975303947503136 2315 12-13 0.5186505848094569 2315 14-15 0.7480562424880972 2315 16-17 0.7925117927409246 2315 18-19 0.7125761023280432 2315 20-21 0.7840182110785818 2315 22-23 0.6001328765961418 2315 24-25 0.7981062899960136 2315 26-27 0.8325812114075362 2315 28-29 0.8379613254210483 2315 30-31 0.8600630368217708 2315 32-33 0.9006940080156625 2315 34-35 0.9791312556028515 2315 36-37 1.040073019970535 2315 38-39 1.0719705066512049 2315 40-41 1.0835453961447286 2315 42-43 1.1107659525559583 2315 44-45 1.1785041446944717 2315 46-47 1.1926789684766632 2315 48-49 1.1424795254762614 2315 50-51 1.0247217232281827 2315 52-53 1.0982766584064976 2315 54-55 0.9889386938567455 2315 56-57 0.9322558616809431 2315 58-59 0.8957796261959032 2315 60-61 0.9038190150706811 2315 62-63 0.902653420163837 2315 64-65 0.9469564022571575 2315 66-67 0.9215051704524058 2315 68-69 0.9121974746187718 2315 70-71 0.8265164546176749 2315 72-73 0.6132353476959906 2315 74-75 0.8708503085420034 2315 76-77 0.8628446144372717 2315 78-79 0.881402983782543 2315 80-81 0.6485940468491478 2315 82-83 0.6868130449742651 2315 84-85 0.9670550252869496 2315 86-87 1.0893174767622291 2315 88-89 1.1225090725839095 2315 90-91 1.1937190454476756 2315 92-93 1.2246301385485516 2315 94-95 1.2242660751449321 2315 96-97 1.0591298853956026 2315 98-99 0.4514588537392896 2315 100 1.029348337014305 2316 1 0.30523833397994693 2316 2 0.2584901974106444 2316 3 0.3039616493345534 2316 4 0.14151036774517678 2316 5 0.10474923367055311 2316 6 0.19021781714045716 2316 7 0.12837691242555138 2316 8 0.2384672537180066 2316 9 0.2763521300999088 2316 10-11 0.23293682755358702 2316 12-13 0.041817899446066065 2316 14-15 0.06409485538966209 2316 16-17 0.2140409481649641 2316 18-19 0.25655354956371923 2316 20-21 0.3062861005830513 2316 22-23 0.2863623252793701 2316 24-25 0.3567365394402202 2316 26-27 0.3769367869634621 2316 28-29 0.3312059964026872 2316 30-31 0.22659313660808067 2316 32-33 0.4540812727614423 2316 34-35 0.44915742974824724 2316 36-37 0.455268313204499 2316 38-39 0.6169077695956062 2316 40-41 0.6552667928463904 2316 42-43 0.6030139158345307 2316 44-45 0.4931319523656441 2316 46-47 0.3365101513344726 2316 48-49 0.29967860728476126 2316 50-51 0.35879709664638426 2316 52-53 0.5194866009269887 2316 54-55 0.15790066815979031 2316 56-57 0.05722207597043649 2316 58-59 0.09658548719981042 2316 60-61 0.20445461272982612 2316 62-63 0.3758147574785511 2316 64-65 0.264440892802952 2316 66-67 0.19737263205392708 2316 68-69 0.16632309393248335 2316 70-71 -0.06708759910610951 2316 72-73 -0.08113192776244915 2316 74-75 0.11460485846799173 2316 76-77 0.24989310308592394 2316 78-79 0.31513096419428877 2316 80-81 0.48091737221254505 2316 82-83 0.4639287907763787 2316 84-85 0.5671634501889393 2316 86-87 0.5894479991695043 2316 88-89 0.5133747551555956 2316 90-91 0.41185182262710285 2316 92-93 0.43167337198882194 2316 94-95 0.5884107634021873 2316 96-97 0.5110268838312315 2316 98-99 0.459141323999674 2316 100 0.34576999110342754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 22.0 1 631.0 2 263.0 3 200.0 4 199.0 5 155.0 6 177.0 7 643.0 8 2192.0 9 4019.0 10 6568.0 11 10314.0 12 15156.0 13 19581.0 14 23463.0 15 27943.0 16 32991.0 17 40690.0 18 48974.0 19 59100.0 20 72718.0 21 88664.0 22 99373.0 23 102893.0 24 102802.0 25 107262.0 26 119584.0 27 138412.0 28 162923.0 29 194846.0 30 237112.0 31 296169.0 32 376352.0 33 495260.0 34 702657.0 35 985459.0 36 1495266.0 37 2067673.0 38 1734309.0 39 472344.0 40 396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29777591959821 19.371696101759124 12.671011595988304 23.659516382654363 2 15.839896119452563 19.16103836799293 40.80136726498706 24.197698247567452 3 19.6764003458618 24.91310918312578 28.42781759038269 26.98267288062973 4 12.917230030527504 15.574335317650212 37.91066107740112 33.59777357442116 5 13.736081167005306 37.519853663191185 34.19886233398161 14.545202835821891 6 31.742232334909616 36.499646144053315 17.30284015091922 14.455281370117849 7 28.417514858550007 31.568221861889906 21.789026094992977 18.22523718456711 8 26.759406188293095 35.27286642253793 19.238352768313305 18.72937462085567 9 26.4500807181132 15.612754187116144 20.726293798460482 37.210871296310174 10-11 26.30060823116333 25.764487969808087 26.70413435999145 21.230769439037132 12-13 26.129229989104115 23.223060332740385 27.545381160750516 23.10232851740499 14-15 23.37845225071005 24.570251580812048 25.20221869816195 26.84907747031595 16-17 22.529045647866937 27.558017460185724 26.19767422132016 23.715262670627187 18-19 22.120946234796115 27.30553228365853 28.362072649180163 22.211448832365193 20-21 23.316212003242025 26.40879726704533 28.05029675312506 22.224693976587588 22-23 23.53675743293999 26.151450486392747 27.779421128072666 22.5323709525946 24-25 22.991701708768026 26.114893296323988 28.37471666644027 22.518688328467714 26-27 22.959501399516277 26.37343169222195 27.645499058489655 23.021567849772122 28-29 22.387865410795165 26.728219883502042 28.0525801649135 22.831334540789292 30-31 23.08952380363129 26.455203972223206 27.955397658804387 22.499874565341113 32-33 23.20159984229358 26.61207851153835 27.400678286493452 22.78564335967462 34-35 23.22085244238088 26.51716072715122 27.93655254799547 22.325434282472425 36-37 22.972047707174887 26.24196264506782 27.689324629183858 23.096665018573436 38-39 23.090033905914893 26.409580609534967 27.982697885251433 22.517687599298707 40-41 22.91747436990446 25.755270608058655 28.33128881688822 22.99596620514866 42-43 22.879618260969323 25.909160811414928 27.88283633894355 23.3283845886722 44-45 22.923586562080644 26.510888387291576 27.79962978927191 22.765895261355865 46-47 22.92733394630905 26.37414740737961 27.342127484766188 23.356391161545147 48-49 23.441249124166884 26.368451171203787 27.258310188905334 22.931989515724 50-51 23.12532842689139 26.323218953355763 27.312147069501535 23.239305550251306 52-53 22.851796489334507 26.68377588169259 27.084589082705328 23.37983854626757 54-55 22.709592492093208 26.60119742136405 27.17221051866181 23.516999567880937 56-57 23.335002128499486 26.359545504321424 26.853439575392756 23.452012791786338 58-59 22.76632453602912 26.619723063135158 27.35517403368289 23.25877836715283 60-61 22.800435797629255 27.02134795428128 26.973462035883404 23.204754212206065 62-63 22.99936009920517 26.581818376943385 26.7172141725288 23.701607351322647 64-65 22.58923075182786 26.619691061644097 26.999818913074314 23.79125927345373 66-67 22.856151509903892 26.381961463872756 26.966703012382006 23.795184013841343 68-69 22.57805198679323 26.41114849494738 27.176421785080063 23.83437773317933 70-71 22.583683181180483 26.18426141342189 27.21099641111734 24.02105899428029 72-73 23.001792155420574 26.286673888925044 27.262505561503193 23.44902839415119 74-75 23.149964427389744 26.465016512004652 26.97553434933497 23.409484711270633 76-77 23.004169529141365 26.349678168578215 26.724136264172273 23.92201603810815 78-79 22.870924640948438 26.374969552647855 26.825003048964852 23.92910275743886 80-81 22.62588873076555 26.708004004373308 27.146857911312967 23.519249353548176 82-83 23.21863587769773 26.080265145135872 27.82348980791827 22.87760916924813 84-85 23.318080228806718 26.32823016280757 26.998840876193388 23.354848732192327 86-87 22.552783327427473 26.756424452955564 27.307407069234092 23.38338515038287 88-89 23.14800067190089 25.909339301488938 27.271760948298677 23.6708990783115 90-91 23.06849344734253 25.998183505926377 27.238816377527584 23.694506669203513 92-93 23.259126954394834 26.103560871740463 27.220296461364068 23.417015712500636 94-95 22.594872932738454 26.081627690736248 27.64681384971879 23.676685526806512 96-97 22.83864220535979 25.986370347682158 27.320069721772768 23.85491772518528 98-99 22.445856458449338 26.270288086501655 27.23480157415315 24.04905388089586 100 23.06499713033537 25.8890739259642 26.690095170764383 24.355833772936045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 802.0 1 619.5 2 698.5 3 1026.5 4 893.5 5 769.5 6 1130.5 7 2048.0 8 3395.0 9 4283.5 10 4190.0 11 4088.5 12 4602.5 13 5437.5 14 6415.0 15 7418.0 16 8390.5 17 9400.0 18 10335.5 19 11100.5 20 11974.0 21 13164.5 22 14920.5 23 17179.5 24 20033.5 25 23810.5 26 29230.0 27 35930.0 28 45470.5 29 61674.5 30 83705.5 31 105544.5 32 132691.0 33 161407.5 34 184198.0 35 207747.5 36 230021.0 37 264079.5 38 285645.5 39 282940.0 40 277164.5 41 271126.5 42 275831.5 43 292964.0 44 317110.0 45 358510.0 46 400676.5 47 432761.0 48 457516.0 49 462058.0 50 453944.0 51 462526.5 52 464409.5 53 445961.5 54 426411.5 55 381447.0 56 338385.5 57 291461.0 58 243886.5 59 223734.5 60 189153.0 61 144073.0 62 112784.5 63 91802.5 64 68892.0 65 43902.5 66 29891.0 67 21948.0 68 16515.5 69 12512.0 70 9776.5 71 7344.5 72 5477.5 73 4290.5 74 3351.0 75 2409.5 76 1645.0 77 1105.5 78 768.5 79 545.0 80 371.5 81 265.0 82 193.5 83 151.0 84 125.0 85 101.0 86 79.0 87 66.5 88 65.5 89 55.5 90 48.5 91 41.0 92 28.5 93 17.5 94 15.0 95 10.0 96 9.5 97 11.5 98 7.0 99 5.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05689289955155521 2 0.13423863217329235 3 0.016808826422044596 4 0.015861578009531446 5 0.018809647048475438 6 0.052088996888095644 7 0.057192539355513446 8 0.048676969443022765 9 0.040693018537554776 10-11 0.03910299441655056 12-13 0.04097332674125765 14-15 0.018766150947900854 16-17 0.017606254932578627 18-19 0.01791072763660071 20-21 0.021501572384035772 22-23 0.02633447244787838 24-25 0.0251455790321731 26-27 0.01935576475568965 28-29 0.019027127551348354 30-31 0.016663839420129316 32-33 0.024024346217361613 34-35 0.02403884491755314 36-37 0.024058176517808512 38-39 0.02203319139105846 40-41 0.025527378137216665 42-43 0.032878219134321277 44-45 0.04202206605511149 46-47 0.047778050031148045 48-49 0.04033538393283042 50-51 0.027934162369010283 52-53 0.040156566630468245 54-55 0.03574412887217994 56-57 0.038300733005952686 58-59 0.043312450372157474 60-61 0.023410567909253603 62-63 0.02653745425055977 64-65 0.025672365139131945 66-67 0.03294104683515123 68-69 0.03953312252223255 70-71 0.04093949644081075 72-73 0.044230701384287564 74-75 0.02731071826077459 76-77 0.020694478073374056 78-79 0.0201096971656491 80-81 0.02477827862732106 82-83 0.020655814872863314 84-85 0.012599370466437684 86-87 0.014580859492613153 88-89 0.01901746175122067 90-91 0.02129859058135438 92-93 0.02010486426558526 94-95 0.024285322820809113 96-97 0.025561208437663564 98-99 0.02826279957335158 100 0.02979966179365353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0345755E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.1852252171893 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70792928238235 45.710214786524155 2 14.638877555102878 17.913660402716538 3 5.01022536502511 9.196553020438072 4 2.186254278226972 5.350658415814436 5 1.104281070439127 3.378284299894843 6 0.6494234600374555 2.384107239823083 7 0.38683869687069766 1.656816895353036 8 0.2818533850436225 1.3796210273696974 9 0.1800589319642126 0.9915251674137222 >10 0.8114780385951516 8.224889897772341 >50 0.030633593120294365 1.2757599791323255 >100 0.011205478928579144 1.129178650618656 >500 5.646104933446502E-4 0.22420152876627314 >1k 2.717388473356758E-4 0.2522097233873998 >5k 4.180596913152918E-5 0.1632968235291928 >10k+ 6.270895369729377E-5 0.7690221414463373 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26102 0.2522967149328396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21501 0.20782436854535993 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12526 0.12107381239938506 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.66580012768522E-6 2.899740038305566E-5 2 0.0 0.0 0.0 9.66580012768522E-6 2.899740038305566E-5 3 0.0 0.0 0.0 9.66580012768522E-6 2.899740038305566E-5 4 0.0 0.0 0.0 9.66580012768522E-6 2.899740038305566E-5 5 0.0 0.0 0.0 9.66580012768522E-6 2.899740038305566E-5 6 5.799480076611132E-5 0.0 0.0 9.66580012768522E-6 2.899740038305566E-5 7 5.799480076611132E-5 9.66580012768522E-6 0.0 9.66580012768522E-6 3.866320051074088E-5 8 5.799480076611132E-5 9.66580012768522E-6 0.0 9.66580012768522E-6 3.866320051074088E-5 9 5.799480076611132E-5 9.66580012768522E-6 0.0 1.933160025537044E-5 4.83290006384261E-5 10-11 5.799480076611132E-5 9.66580012768522E-6 0.0 1.933160025537044E-5 4.83290006384261E-5 12-13 5.799480076611132E-5 9.66580012768522E-6 0.0 5.799480076611132E-5 4.83290006384261E-5 14-15 5.799480076611132E-5 1.4498700191527828E-5 0.0 5.799480076611132E-5 7.249350095763914E-5 16-17 1.0632380140453742E-4 1.933160025537044E-5 0.0 5.799480076611132E-5 9.66580012768522E-5 18-19 1.0632380140453742E-4 2.899740038305566E-5 0.0 5.799480076611132E-5 9.66580012768522E-5 20-21 1.1115670146838002E-4 2.899740038305566E-5 0.0 1.3532120178759308E-4 9.66580012768522E-5 22-23 1.1598960153222264E-4 2.899740038305566E-5 0.0 2.561437033836583E-4 1.3532120178759308E-4 24-25 1.2082250159606525E-4 2.899740038305566E-5 0.0 5.46117707214215E-4 1.8365020242601916E-4 26-27 1.2565540165990785E-4 2.899740038305566E-5 0.0 0.001188893415705282 1.8365020242601916E-4 28-29 1.2565540165990785E-4 2.899740038305566E-5 0.0 0.003938813552031727 1.884831024898618E-4 30-31 1.2565540165990785E-4 2.899740038305566E-5 0.0 0.01611288881285126 2.0781470274523222E-4 32-33 1.2565540165990785E-4 2.899740038305566E-5 0.0 0.04568540430350419 2.3197920306444527E-4 34-35 1.2565540165990785E-4 2.899740038305566E-5 0.0 0.08905101657636394 2.3197920306444527E-4 36-37 1.2565540165990785E-4 3.866320051074088E-5 0.0 0.16417361516873347 2.3197920306444527E-4 38-39 1.2565540165990785E-4 4.83290006384261E-5 0.0 0.27103870138042124 2.416450031921305E-4 40-41 1.2565540165990785E-4 4.83290006384261E-5 0.0 0.38362594126769867 2.416450031921305E-4 42-43 1.2565540165990785E-4 4.83290006384261E-5 0.0 0.4939175536246509 2.464779032559731E-4 44-45 1.2565540165990785E-4 4.83290006384261E-5 0.0 0.6221585568187145 2.658095035113435E-4 46-47 1.2565540165990785E-4 4.83290006384261E-5 0.0 0.7758737762492925 2.948069038943992E-4 48-49 1.3048830172375048E-4 4.83290006384261E-5 0.0 0.9458565372947648 3.1897140421361227E-4 50-51 1.3532120178759308E-4 4.83290006384261E-5 0.0 1.1203725586001214 3.2380430427745487E-4 52-53 1.3532120178759308E-4 4.83290006384261E-5 0.0 1.288320668718716 3.4796880459666787E-4 54-55 1.3532120178759308E-4 8.215930108532437E-5 0.0 1.4693804367105154 3.673004048520383E-4 56-57 1.3532120178759308E-4 8.699220114916698E-5 0.0 1.6785628501738152 3.673004048520383E-4 58-59 1.3532120178759308E-4 9.66580012768522E-5 0.0 1.915138141198975 3.673004048520383E-4 60-61 1.4015410185143568E-4 9.66580012768522E-5 0.0 2.1565366664878494 3.769662049797236E-4 62-63 1.546528020429635E-4 9.66580012768522E-5 0.0 2.399491385597281 4.1562940549046444E-4 64-65 1.546528020429635E-4 9.66580012768522E-5 0.0 2.6462689286572125 4.1562940549046444E-4 66-67 1.546528020429635E-4 1.0632380140453742E-4 0.0 2.9259681869520398 4.2046230555430704E-4 68-69 1.546528020429635E-4 1.0632380140453742E-4 0.0 3.2335097825146644 4.252952056181497E-4 70-71 1.5948570210680613E-4 1.1598960153222264E-4 0.0 3.5652883718974593 4.6879130619273315E-4 72-73 1.8365020242601916E-4 1.2565540165990785E-4 0.0 3.8943170411439283 5.992796079164836E-4 74-75 1.8365020242601916E-4 1.2565540165990785E-4 0.0 4.210074566815084 5.992796079164836E-4 76-77 1.8365020242601916E-4 1.3532120178759308E-4 0.0 4.563330564081596 5.992796079164836E-4 78-79 1.8365020242601916E-4 1.546528020429635E-4 0.0 4.9481115684645545 5.992796079164836E-4 80-81 1.8365020242601916E-4 1.7398440229833396E-4 0.0 5.357293885269852 6.137783081080115E-4 82-83 1.8365020242601916E-4 1.7398440229833396E-4 0.0 5.7692357880116045 6.572744086825949E-4 84-85 1.8365020242601916E-4 1.7881730236217656E-4 0.0 6.169863871703901 6.572744086825949E-4 86-87 1.8365020242601916E-4 1.8365020242601916E-4 0.0 6.59027784825757 6.572744086825949E-4 88 1.8365020242601916E-4 1.8365020242601916E-4 0.0 6.930195041347877 6.669402088102801E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16175 0.0 45.853592 1 GTATCAA 28940 0.0 40.19864 1 CAACGCA 31900 0.0 34.275562 5 GTACTAG 7200 0.0 33.98852 1 ATCAACG 32445 0.0 33.80139 3 GTGGTAT 5840 0.0 32.878254 1 TATCAAC 35915 0.0 32.10267 2 AACGCAG 36310 0.0 30.863823 6 TCAACGC 37560 0.0 29.535873 4 GCATCGT 8175 0.0 27.714827 8 ACGCAGA 39595 0.0 27.57927 7 CTGTGCG 11035 0.0 27.51776 9 GTACATG 42615 0.0 27.497822 1 TAGTACT 11170 0.0 27.31209 4 TAGGCAT 9635 0.0 27.0286 5 TACATGG 42875 0.0 26.93527 2 CGCAGAG 40550 0.0 26.916853 8 TACCTGG 6500 0.0 26.28114 2 ACATGGG 42800 0.0 25.821201 3 CTAGTAC 12075 0.0 25.771427 3 >>END_MODULE