FastQCFastQC Report
Mon 6 Jun 2016
ERR1211237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10345755
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244370.2362031577202437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209380.20238252307347313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124930.12075484099517145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163550.047.461781
GTATCAA285100.038.7046661
CAACGCA326700.032.3164945
ATCAACG333700.031.6763973
TATCAAC363250.030.170862
GTGGTAT56800.029.385121
AACGCAG373050.029.145176
TCAACGC379150.028.1891484
CTGTGCG113650.027.2860459
TAGTACT114050.027.24014
TAGGCAT99050.026.9915665
TACCTGG65700.026.96752
GTACATG411600.026.4551731
GGGCACC101400.026.00927494
ACGCAGA406200.025.9453567
TACATGG417200.025.649772
CTAGGCA109700.025.3639954
CGCAGAG415650.025.2308868
GCATCGT93850.025.032368
CTAGTAC124500.024.9914363
Produced by FastQC (version 0.11.5)