##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10345755 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.159164797542566 33.0 31.0 34.0 30.0 34.0 2 32.30404663555245 34.0 31.0 34.0 30.0 34.0 3 32.38338758263655 34.0 31.0 34.0 30.0 34.0 4 35.99591484623404 37.0 35.0 37.0 35.0 37.0 5 35.87748492014357 37.0 35.0 37.0 35.0 37.0 6 35.90206891618833 37.0 35.0 37.0 35.0 37.0 7 35.83851367058276 37.0 35.0 37.0 35.0 37.0 8 35.827508867163395 37.0 35.0 37.0 35.0 37.0 9 37.43412404411278 39.0 37.0 39.0 34.0 39.0 10-11 37.413862932188124 39.0 37.0 39.0 34.0 39.0 12-13 37.31351612327955 39.0 37.0 39.0 34.0 39.0 14-15 38.54580762834612 40.0 38.0 41.0 34.0 41.0 16-17 38.49882120734543 40.0 38.0 41.0 34.0 41.0 18-19 38.512224917369494 40.0 38.0 41.0 34.0 41.0 20-21 38.468698611169515 40.0 38.0 41.0 34.0 41.0 22-23 38.33834543733154 40.0 38.0 41.0 34.0 41.0 24-25 38.27275587910211 40.0 38.0 41.0 34.0 41.0 26-27 38.1367598111496 40.0 38.0 41.0 33.0 41.0 28-29 38.117919813488726 40.0 37.5 41.0 33.5 41.0 30-31 38.14435374701991 40.0 37.5 41.0 33.5 41.0 32-33 38.02777124530786 40.0 37.0 41.0 33.0 41.0 34-35 37.89342769087418 40.0 37.0 41.0 33.0 41.0 36-37 37.75528861837536 40.0 37.0 41.0 33.0 41.0 38-39 37.669669057502325 40.0 37.0 41.0 33.0 41.0 40-41 37.565953475604246 40.0 36.5 41.0 32.5 41.0 42-43 37.42191662184152 40.0 36.0 41.0 32.0 41.0 44-45 37.27988952957034 39.5 36.0 41.0 31.5 41.0 46-47 37.04528635174523 39.0 36.0 41.0 31.0 41.0 48-49 36.806402094385575 39.0 35.0 41.0 31.0 41.0 50-51 36.599318416104 39.0 35.0 40.5 30.0 41.0 52-53 36.37949062199907 39.0 35.0 40.0 30.0 41.0 54-55 36.12786215215806 38.0 35.0 40.0 29.5 41.0 56-57 35.85515044576253 38.0 35.0 40.0 29.0 41.0 58-59 35.56523743313079 37.5 34.0 40.0 28.5 41.0 60-61 35.29179905188167 37.0 34.0 40.0 28.0 41.0 62-63 34.97736685239502 36.5 34.0 39.5 28.0 41.0 64-65 34.593982991091515 36.0 33.5 39.0 27.0 41.0 66-67 34.28407617423765 35.5 33.0 39.0 26.5 40.0 68-69 33.95624364775698 35.0 33.0 38.5 26.0 40.0 70-71 33.6458286514614 35.0 33.0 37.5 26.0 40.0 72-73 33.320762911938274 35.0 32.5 37.0 26.0 39.5 74-75 32.99771447323081 35.0 32.0 36.5 26.0 39.0 76-77 32.02215309564164 34.0 30.5 35.5 24.5 38.0 78-79 32.53335923767767 35.0 32.0 36.0 25.5 38.0 80-81 32.47657739816958 35.0 32.0 35.0 25.5 37.0 82-83 32.24585474912174 35.0 32.0 35.0 25.0 37.0 84-85 32.01336804322159 34.0 32.0 35.0 25.0 36.5 86-87 31.793491871787026 34.0 31.0 35.0 25.0 36.0 88-89 31.588612817527576 34.0 31.0 35.0 24.0 36.0 90-91 31.384810968363354 34.0 31.0 35.0 24.0 35.0 92-93 31.210926268793337 34.0 31.0 35.0 23.5 35.0 94-95 31.035184237399783 34.0 31.0 35.0 23.0 35.0 96-97 30.874122961543165 34.0 30.0 35.0 22.5 35.0 98-99 30.707600750259406 34.0 30.0 35.0 20.0 35.0 100 28.86384241652736 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33383810723099927 1101 2 -0.3214183161015711 1101 3 -0.3997628119741421 1101 4 -0.19242359023137112 1101 5 -0.247245204212021 1101 6 -0.35475186222709 1101 7 -0.3253600373475294 1101 8 -0.27667748532492453 1101 9 -0.5115022698163756 1101 10-11 -0.48232491649959996 1101 12-13 -0.48848334175173136 1101 14-15 -0.6796478853686381 1101 16-17 -0.653579379835854 1101 18-19 -0.7103785360804196 1101 20-21 -0.7621920805891307 1101 22-23 -0.7252215227775523 1101 24-25 -0.7423846200019213 1101 26-27 -0.809166421259377 1101 28-29 -0.8504524018384885 1101 30-31 -0.6746462243147846 1101 32-33 -0.7027873267408253 1101 34-35 -0.7509984177062847 1101 36-37 -0.6206942670613742 1101 38-39 -0.728239461876008 1101 40-41 -0.7681236633322044 1101 42-43 -0.7803573617581137 1101 44-45 -0.8385944749377998 1101 46-47 -0.8067473166533503 1101 48-49 -0.8236647252123177 1101 50-51 -0.8262588471350725 1101 52-53 -0.8342561205042003 1101 54-55 -0.8054765123394532 1101 56-57 -0.8559480910211406 1101 58-59 -0.8907886787005452 1101 60-61 -0.9188288231742519 1101 62-63 -1.0218319737701265 1101 64-65 -0.9328147110685236 1101 66-67 -1.0262510552401523 1101 68-69 -1.0189602171378453 1101 70-71 -1.0372904052448533 1101 72-73 -1.0799663997554774 1101 74-75 -1.1182761610285041 1101 76-77 -1.1407927589120384 1101 78-79 -0.9693208321001734 1101 80-81 -0.9118829480583308 1101 82-83 -0.9150674597137964 1101 84-85 -0.854250003226209 1101 86-87 -1.0377706083442746 1101 88-89 -1.0159368463399048 1101 90-91 -1.0262387887507458 1101 92-93 -1.0553078264312035 1101 94-95 -1.0176747840184248 1101 96-97 -1.1106653304675476 1101 98-99 -1.341824998412683 1101 100 -1.0919826419601009 1102 1 -0.45360408997389 1102 2 -0.3901230841539238 1102 3 -0.3886825260334419 1102 4 -0.2462535614098016 1102 5 -0.3452307708922575 1102 6 -0.3526336023771961 1102 7 -0.3656039203081818 1102 8 -0.4244736788212151 1102 9 -0.5126227832492347 1102 10-11 -0.5371542234036113 1102 12-13 -0.5403355796293923 1102 14-15 -0.6988404433233129 1102 16-17 -0.7299638508365902 1102 18-19 -0.6923333910300542 1102 20-21 -0.7865075455997683 1102 22-23 -0.7285857043825175 1102 24-25 -0.7875761449173453 1102 26-27 -0.8886953759952334 1102 28-29 -0.8471471434239319 1102 30-31 -0.7052760913884129 1102 32-33 -0.6783478673835575 1102 34-35 -0.7879420873172478 1102 36-37 -0.8382688828921232 1102 38-39 -0.9349242429649891 1102 40-41 -0.8573532710668701 1102 42-43 -0.792082759917804 1102 44-45 -0.8502940256238674 1102 46-47 -0.9413631989881424 1102 48-49 -0.9578653568544482 1102 50-51 -0.9748923856585137 1102 52-53 -1.0630322621978578 1102 54-55 -0.9418934884895549 1102 56-57 -0.9879260718241909 1102 58-59 -1.0293697048857524 1102 60-61 -1.0201493983152687 1102 62-63 -0.9872120655640586 1102 64-65 -0.9999533937029881 1102 66-67 -1.025181625917078 1102 68-69 -1.1694517195744538 1102 70-71 -1.1304669448244482 1102 72-73 -1.1154223747547576 1102 74-75 -1.2449075325269021 1102 76-77 -0.9858696138014018 1102 78-79 -0.8508083726450764 1102 80-81 -0.8951769340333229 1102 82-83 -0.8158612701295809 1102 84-85 -0.9158947968595292 1102 86-87 -0.8978029868014232 1102 88-89 -1.0079314991593549 1102 90-91 -1.0654265846618998 1102 92-93 -1.0799770907557757 1102 94-95 -1.133991978513503 1102 96-97 -1.293624002879767 1102 98-99 -1.2596452523436916 1102 100 -1.2253958758484949 1103 1 -0.3672249916016881 1103 2 -0.3553149660103685 1103 3 -0.39700546001436265 1103 4 -0.25682947179920035 1103 5 -0.2529900100135052 1103 6 -0.33458097643271856 1103 7 -0.3792547296997171 1103 8 -0.3389168411108372 1103 9 -0.5599423196450886 1103 10-11 -0.5568052074636967 1103 12-13 -0.5594673698693597 1103 14-15 -0.7277946576247274 1103 16-17 -0.7315213872211785 1103 18-19 -0.6750324934590779 1103 20-21 -0.7159595540064032 1103 22-23 -0.7343252510331055 1103 24-25 -0.8713689373040552 1103 26-27 -0.8440571540084036 1103 28-29 -0.9203304327144934 1103 30-31 -0.8375053692184622 1103 32-33 -0.841893791126644 1103 34-35 -0.9265886877601162 1103 36-37 -0.9507189812136119 1103 38-39 -0.8514481641049585 1103 40-41 -0.9654511981455585 1103 42-43 -0.8529560181624376 1103 44-45 -0.940640877927521 1103 46-47 -0.9572816851188932 1103 48-49 -0.9620515873380455 1103 50-51 -0.9840608224876561 1103 52-53 -0.9685976473983615 1103 54-55 -0.9468879329026976 1103 56-57 -1.0127358295983484 1103 58-59 -0.9850753940095274 1103 60-61 -1.033051606463843 1103 62-63 -0.9251929872505755 1103 64-65 -0.938945111032929 1103 66-67 -1.169108242587221 1103 68-69 -1.0934354584956765 1103 70-71 -1.0490931146811633 1103 72-73 -1.0339514288963798 1103 74-75 -0.9720955096960324 1103 76-77 -0.9244324127804369 1103 78-79 -0.7511701224859024 1103 80-81 -0.7301540918942528 1103 82-83 -0.8273286155619992 1103 84-85 -0.860600739534437 1103 86-87 -0.8875209308962155 1103 88-89 -0.9085680026330039 1103 90-91 -0.9470060842083257 1103 92-93 -1.0214510172484879 1103 94-95 -1.0851645735810855 1103 96-97 -1.149680212346805 1103 98-99 -1.1344095737563684 1103 100 -1.1809979020036998 1104 1 -0.32860621226923925 1104 2 -0.3239439850347523 1104 3 -0.3628024165503696 1104 4 -0.2494030477052931 1104 5 -0.29117506837756224 1104 6 -0.3315397222892571 1104 7 -0.37011261021476116 1104 8 -0.38457626195561545 1104 9 -0.5265560666503077 1104 10-11 -0.5401118074702609 1104 12-13 -0.565985207281436 1104 14-15 -0.7263963817437968 1104 16-17 -0.698389748104681 1104 18-19 -0.7213995470314813 1104 20-21 -0.7821491742844913 1104 22-23 -0.7070360453750197 1104 24-25 -0.7301483682134773 1104 26-27 -0.7908576550531663 1104 28-29 -0.8703573775448703 1104 30-31 -0.7996883052532127 1104 32-33 -0.8024079965112279 1104 34-35 -0.8312773945614182 1104 36-37 -0.7713186924017918 1104 38-39 -0.7883599671002841 1104 40-41 -0.8492301258222739 1104 42-43 -0.7875907368444075 1104 44-45 -0.8453805753314967 1104 46-47 -0.880533914111659 1104 48-49 -0.8487856288438067 1104 50-51 -0.8838403299759747 1104 52-53 -0.8313768157515895 1104 54-55 -0.867407914523767 1104 56-57 -0.8747862732089331 1104 58-59 -0.8513632868865955 1104 60-61 -0.9191302092272409 1104 62-63 -0.9932455203709409 1104 64-65 -0.9527710909692573 1104 66-67 -0.9504832494246216 1104 68-69 -0.9798754151739075 1104 70-71 -1.0612195573728016 1104 72-73 -1.0342913516610963 1104 74-75 -1.0364464488814527 1104 76-77 -0.907880683738437 1104 78-79 -0.7584608378421578 1104 80-81 -0.7739842289704697 1104 82-83 -0.7022651863591847 1104 84-85 -0.7619021868755098 1104 86-87 -0.799347764140208 1104 88-89 -0.8795086539911843 1104 90-91 -0.8311093125556468 1104 92-93 -0.9192507853238396 1104 94-95 -0.9096618222108717 1104 96-97 -0.9130404941571264 1104 98-99 -1.0141310782034125 1104 100 -1.034694671468415 1105 1 -0.3653733511971424 1105 2 -0.37504746899862695 1105 3 -0.45006735929226593 1105 4 -0.2552158333267869 1105 5 -0.31882862760903663 1105 6 -0.34338324914759255 1105 7 -0.3848358930197122 1105 8 -0.4407199816491101 1105 9 -0.5585868122413018 1105 10-11 -0.512962381743371 1105 12-13 -0.5888118466373484 1105 14-15 -0.7494051249940199 1105 16-17 -0.7191159335787773 1105 18-19 -0.7152872623942699 1105 20-21 -0.8489204746633305 1105 22-23 -0.786705068408132 1105 24-25 -0.7722820458038058 1105 26-27 -0.7910248535122761 1105 28-29 -0.823439691134837 1105 30-31 -0.7673472507032599 1105 32-33 -0.7415000449131313 1105 34-35 -0.7196767745642916 1105 36-37 -0.6996750660747395 1105 38-39 -0.7775034591921965 1105 40-41 -0.816744058524236 1105 42-43 -0.8134772852937644 1105 44-45 -0.8800073832190591 1105 46-47 -0.8448814657069406 1105 48-49 -0.8510921021374429 1105 50-51 -0.9344627229663232 1105 52-53 -0.9350827606793288 1105 54-55 -0.8905961750418072 1105 56-57 -0.919310257735475 1105 58-59 -1.0113973510331675 1105 60-61 -0.9352450000899779 1105 62-63 -1.0144457967606044 1105 64-65 -0.9907446001633033 1105 66-67 -0.8580508843726378 1105 68-69 -0.876859867802672 1105 70-71 -0.874956205988191 1105 72-73 -0.9253091413357311 1105 74-75 -0.975699514782896 1105 76-77 -0.9461530991796252 1105 78-79 -0.8404556745907783 1105 80-81 -0.8741759184714795 1105 82-83 -0.7650265420644544 1105 84-85 -0.8323254266540836 1105 86-87 -0.8662959736051334 1105 88-89 -0.9103155588095575 1105 90-91 -0.8326449495974586 1105 92-93 -0.913001075376279 1105 94-95 -0.8929553189813504 1105 96-97 -0.9627379526219961 1105 98-99 -1.0263632079715315 1105 100 -1.0969155515667879 1106 1 -0.3908771196238554 1106 2 -0.3606855420221535 1106 3 -0.4377086583695551 1106 4 -0.26805709485726226 1106 5 -0.30447578838255396 1106 6 -0.35361865523385916 1106 7 -0.41183832782181895 1106 8 -0.44095958846386907 1106 9 -0.5214535617151697 1106 10-11 -0.5565824660478 1106 12-13 -0.6481963067928334 1106 14-15 -0.7341484337508604 1106 16-17 -0.7248557467255381 1106 18-19 -0.6975410638195925 1106 20-21 -0.7065872298389806 1106 22-23 -0.6946740795489319 1106 24-25 -0.6900513666906534 1106 26-27 -0.7523859501998444 1106 28-29 -0.7706429127866201 1106 30-31 -0.793068242350742 1106 32-33 -0.7856716874027398 1106 34-35 -0.7064472960523176 1106 36-37 -0.7895712785539999 1106 38-39 -0.7678435221470252 1106 40-41 -0.7633981157843479 1106 42-43 -0.7717594111236394 1106 44-45 -0.80136253636649 1106 46-47 -0.7957842193948395 1106 48-49 -0.8422372216766618 1106 50-51 -0.9460858793851727 1106 52-53 -0.9424278344180266 1106 54-55 -0.9537627693566861 1106 56-57 -0.9505567151106433 1106 58-59 -0.8333258090590618 1106 60-61 -0.9349047943740771 1106 62-63 -0.9850171601750475 1106 64-65 -0.9320698347913563 1106 66-67 -0.9401607202405131 1106 68-69 -0.9637314355387616 1106 70-71 -0.9649498978548863 1106 72-73 -0.9347680484691239 1106 74-75 -0.9483808988530704 1106 76-77 -0.9637317322091512 1106 78-79 -0.803108255367718 1106 80-81 -0.7627503009252905 1106 82-83 -0.8067994301585486 1106 84-85 -0.7541511226993691 1106 86-87 -0.8246125386496708 1106 88-89 -0.8777459077048562 1106 90-91 -0.908358255109782 1106 92-93 -0.9049226234626815 1106 94-95 -0.876441090023647 1106 96-97 -0.9206470853471416 1106 98-99 -0.8951142344052734 1106 100 -0.998944050014213 1107 1 -0.4605590172234244 1107 2 -0.4387050246076356 1107 3 -0.43241281169333945 1107 4 -0.2916458493648193 1107 5 -0.27509724407409664 1107 6 -0.32836939049182945 1107 7 -0.39159105470792355 1107 8 -0.4285373257833598 1107 9 -0.5703988273326672 1107 10-11 -0.606803416697268 1107 12-13 -0.6022416061445668 1107 14-15 -0.7208932663369652 1107 16-17 -0.7044038853933259 1107 18-19 -0.6974653079293134 1107 20-21 -0.7559055025298207 1107 22-23 -0.8093158415621744 1107 24-25 -0.8065553041674534 1107 26-27 -0.8393157449189417 1107 28-29 -0.8346296602477921 1107 30-31 -0.8713838090264545 1107 32-33 -0.8060839832366682 1107 34-35 -0.8352085260613293 1107 36-37 -0.8905429033099779 1107 38-39 -0.8850741694242714 1107 40-41 -0.8587088671822585 1107 42-43 -0.8037170938938445 1107 44-45 -0.8843413748916973 1107 46-47 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0.8833630385679356 2311 32-33 0.9839650826583934 2311 34-35 1.066545681518818 2311 36-37 1.078402979712898 2311 38-39 1.0911425459924189 2311 40-41 1.0903477564750617 2311 42-43 1.0745305166083696 2311 44-45 1.0527782643661254 2311 46-47 0.7881398450971702 2311 48-49 1.0573237321571227 2311 50-51 1.1254519971180699 2311 52-53 1.0379826619702541 2311 54-55 1.0326648092282085 2311 56-57 1.132312954772253 2311 58-59 1.1371206986418585 2311 60-61 1.1379172797914592 2311 62-63 1.1359999657250412 2311 64-65 1.1791011350645846 2311 66-67 1.1716957974237445 2311 68-69 1.2094862972464 2311 70-71 1.1996969515615774 2311 72-73 1.2058393006581412 2311 74-75 1.2673781302937073 2311 76-77 1.2086209174869218 2311 78-79 0.9748682523117864 2311 80-81 0.9051833657643442 2311 82-83 1.004970184285881 2311 84-85 1.0557828492476382 2311 86-87 1.1505585692320253 2311 88-89 1.121278162098264 2311 90-91 1.1868385416396023 2311 92-93 1.1964695533765308 2311 94-95 1.2329926666881228 2311 96-97 1.2886870678095654 2311 98-99 1.348144592283841 2311 100 1.4227804280425538 2312 1 0.32256461327881425 2312 2 0.3863951776495682 2312 3 0.4744996238419361 2312 4 0.2256591906546106 2312 5 0.3530155876949337 2312 6 0.4500346402991795 2312 7 0.33487651032390175 2312 8 0.4452951283641582 2312 9 0.6089127303188349 2312 10-11 0.6356138116253689 2312 12-13 0.6876005460775474 2312 14-15 0.8819194437900109 2312 16-17 0.8524422714060691 2312 18-19 0.8648174617155249 2312 20-21 0.8753104479615459 2312 22-23 0.9624740623729693 2312 24-25 0.9489959414487146 2312 26-27 1.0048731807607894 2312 28-29 0.9874302433837343 2312 30-31 0.9993551035255024 2312 32-33 0.8326040332015268 2312 34-35 0.9554640968811725 2312 36-37 1.005720285836432 2312 38-39 0.8101336914479305 2312 40-41 0.9670575810729432 2312 42-43 1.0917485715211583 2312 44-45 0.7863169643308012 2312 46-47 0.8415488967366613 2312 48-49 0.9961238221037121 2312 50-51 0.8489642360040222 2312 52-53 1.0323721774545973 2312 54-55 0.9568411686213665 2312 56-57 0.9354874883558679 2312 58-59 1.032125095789958 2312 60-61 1.0953811882358337 2312 62-63 1.1292607332576026 2312 64-65 1.074863233051211 2312 66-67 1.0914695458806278 2312 68-69 1.1483214785082367 2312 70-71 1.0704214153081253 2312 72-73 1.059947637423349 2312 74-75 1.0740848196066537 2312 76-77 1.1030083630269623 2312 78-79 0.9984260334627564 2312 80-81 0.9632138699713622 2312 82-83 0.999939462193673 2312 84-85 1.0485957269121151 2312 86-87 1.0406967018853663 2312 88-89 1.0545654458615203 2312 90-91 1.1420936031071776 2312 92-93 1.1194270576645948 2312 94-95 1.156908918208213 2312 96-97 0.8253050280704031 2312 98-99 0.6041013264688537 2312 100 0.8220476887076025 2313 1 0.033720585249881196 2313 2 0.2338732501948826 2313 3 0.3185589519790142 2313 4 0.22688174748214607 2313 5 0.35381639907279094 2313 6 0.24405205605215485 2313 7 0.2318931930937822 2313 8 0.396430858581553 2313 9 0.5917055186404099 2313 10-11 0.3650003565426587 2313 12-13 0.4395031368405711 2313 14-15 0.5002300525128547 2313 16-17 0.7992643681112739 2313 18-19 0.7185265776290493 2313 20-21 0.6526935068731561 2313 22-23 0.6341270019500271 2313 24-25 0.5437464837147772 2313 26-27 0.7296631752347409 2313 28-29 0.5772059564279246 2313 30-31 0.8297685718963663 2313 32-33 0.9095321629686168 2313 34-35 0.9203071645875127 2313 36-37 1.0412569678111652 2313 38-39 1.0007663002833738 2313 40-41 0.9849987731217169 2313 42-43 1.0651160164520306 2313 44-45 0.9266972216567027 2313 46-47 0.935026194406511 2313 48-49 1.0540154444031558 2313 50-51 1.0093550751562574 2313 52-53 1.0187786497993585 2313 54-55 1.0211259771131864 2313 56-57 0.7153591742890129 2313 58-59 0.8045442355074215 2313 60-61 0.6788117779123297 2313 62-63 0.972014260155035 2313 64-65 1.0786155488054874 2313 66-67 1.0567551417889476 2313 68-69 1.1586555228111592 2313 70-71 1.0715723181687835 2313 72-73 1.1014890605739254 2313 74-75 1.1601278956381122 2313 76-77 1.1255814972354479 2313 78-79 1.0124857271574754 2313 80-81 0.9147267349319677 2313 82-83 0.971058160627031 2313 84-85 0.9702225204459545 2313 86-87 1.0381518203460516 2313 88-89 1.068752400977175 2313 90-91 0.9505916408364641 2313 92-93 1.0725688421302095 2313 94-95 1.1713956806189607 2313 96-97 1.2562328120252992 2313 98-99 1.2435957502472839 2313 100 1.322262720651203 2314 1 -0.39625481083572467 2314 2 -0.014249969332965406 2314 3 0.2645293431113984 2314 4 0.22894354873359646 2314 5 0.32443951313109665 2314 6 0.4094398339438641 2314 7 0.4310534220124751 2314 8 0.4458569785269546 2314 9 0.4737399393826962 2314 10-11 0.6081283742931518 2314 12-13 0.45151449465290483 2314 14-15 0.772486332929013 2314 16-17 0.8278887891707356 2314 18-19 0.8548452687104557 2314 20-21 0.8609576755386144 2314 22-23 0.9092799995073406 2314 24-25 0.840419097991159 2314 26-27 0.7442715473197907 2314 28-29 0.8310954986634158 2314 30-31 0.8692785275539947 2314 32-33 0.9303462179579256 2314 34-35 0.9103873306781765 2314 36-37 0.8266977814858478 2314 38-39 0.6175396053826958 2314 40-41 0.6282428035199388 2314 42-43 0.8566246199439362 2314 44-45 0.9029449417439182 2314 46-47 0.8608287175459068 2314 48-49 0.8952138416972204 2314 50-51 0.8921560041401975 2314 52-53 0.9293070404039341 2314 54-55 0.839729943496792 2314 56-57 0.8107502617687956 2314 58-59 0.9278043280701596 2314 60-61 1.028423263786749 2314 62-63 0.9821405423541663 2314 64-65 0.9772030935043077 2314 66-67 0.62753891470188 2314 68-69 0.6400791735375009 2314 70-71 0.8330536851135903 2314 72-73 0.9112096622604753 2314 74-75 1.0317344323478252 2314 76-77 0.9208915527991657 2314 78-79 0.7836021410000527 2314 80-81 0.8615654727196969 2314 82-83 0.8732984799857277 2314 84-85 0.9141747287831556 2314 86-87 0.9574201203895001 2314 88-89 0.9517953966755641 2314 90-91 1.022197216515913 2314 92-93 0.909653741211514 2314 94-95 0.8859006502103703 2314 96-97 0.9141220133709211 2314 98-99 1.0626507208189722 2314 100 1.000213743059092 2315 1 0.47075691363080097 2315 2 0.45287811299663616 2315 3 0.4961840238928801 2315 4 0.34255898639711546 2315 5 0.4144378409346814 2315 6 0.46919987582742095 2315 7 0.42572180844071994 2315 8 0.3176894869462714 2315 9 0.5214508411461765 2315 10-11 0.5837529360336049 2315 12-13 0.4357927892691791 2315 14-15 0.656166426698249 2315 16-17 0.8466033678519977 2315 18-19 0.8133957323336389 2315 20-21 0.5349609789543521 2315 22-23 0.8517994699633036 2315 24-25 0.9087944437935604 2315 26-27 0.8934117424152319 2315 28-29 0.8309506495638814 2315 30-31 0.8904904904533737 2315 32-33 0.842905718167799 2315 34-35 0.868985846665943 2315 36-37 0.8608104526007665 2315 38-39 0.934641628197106 2315 40-41 0.954564613478027 2315 42-43 0.9999754364047035 2315 44-45 1.0364395262230275 2315 46-47 0.9730779611641864 2315 48-49 0.920610098440001 2315 50-51 0.9538716543827306 2315 52-53 1.0158187196766661 2315 54-55 0.8544067460088911 2315 56-57 0.8125792783398325 2315 58-59 0.8137695737027286 2315 60-61 0.9437328920112265 2315 62-63 0.9464549485487836 2315 64-65 1.0072535224883907 2315 66-67 0.9921602312844087 2315 68-69 1.0408200491630808 2315 70-71 0.9632139852148995 2315 72-73 1.0789921570026522 2315 74-75 0.5018976067954242 2315 76-77 0.5176191453800989 2315 78-79 0.7508299627060424 2315 80-81 0.8021079925820729 2315 82-83 0.7613268341662405 2315 84-85 0.8387328720925531 2315 86-87 0.8692148377906364 2315 88-89 0.866872252261274 2315 90-91 0.9161448588664172 2315 92-93 0.9432263438460637 2315 94-95 0.9849994038600904 2315 96-97 1.0416608791322979 2315 98-99 1.0791072005361144 2315 100 1.1261721581418058 2316 1 0.2902887896395967 2316 2 0.07593206516963846 2316 3 0.18216218851115684 2316 4 0.013326757862799354 2316 5 0.2794949578260386 2316 6 0.3560818078766914 2316 7 0.4029273128484405 2316 8 0.40802243014422146 2316 9 0.5599035806363659 2316 10-11 0.6031124600791813 2316 12-13 0.3619299152979423 2316 14-15 0.5019134471482047 2316 16-17 0.7326559221192994 2316 18-19 0.6916126596517458 2316 20-21 0.6861041329933357 2316 22-23 0.702402352865029 2316 24-25 0.43353937370150675 2316 26-27 0.7059017769628255 2316 28-29 0.8700223518484904 2316 30-31 0.5033108555412866 2316 32-33 0.6045879902498754 2316 34-35 0.7425319498705747 2316 36-37 0.8167867620267657 2316 38-39 0.6942928195570275 2316 40-41 0.6952468147524229 2316 42-43 0.6910845775455599 2316 44-45 0.5284114916345786 2316 46-47 0.7094194297205618 2316 48-49 0.8288703400917328 2316 50-51 0.8449531911879333 2316 52-53 0.8268410026231976 2316 54-55 0.7199874146922411 2316 56-57 0.839212958785005 2316 58-59 0.6920558595298161 2316 60-61 0.8683297886491204 2316 62-63 0.8874818836427423 2316 64-65 0.9140238526481497 2316 66-67 0.894639139357281 2316 68-69 0.8600406599359474 2316 70-71 0.8175376697634889 2316 72-73 0.8461623033224015 2316 74-75 0.8759323063512312 2316 76-77 0.7376836606857076 2316 78-79 0.46631097426593726 2316 80-81 0.44308145280498223 2316 82-83 0.42238072322579256 2316 84-85 0.5350857192655276 2316 86-87 0.6225417587490512 2316 88-89 0.5944984566740885 2316 90-91 0.46596802683610505 2316 92-93 0.30312825130188514 2316 94-95 0.4097727283214958 2316 96-97 0.6441061847524203 2316 98-99 0.8267177664344807 2316 100 0.4350089949560605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 7.0 12 42.0 13 216.0 14 819.0 15 2343.0 16 5229.0 17 10639.0 18 18473.0 19 27531.0 20 40390.0 21 58338.0 22 72122.0 23 76862.0 24 79221.0 25 87130.0 26 103437.0 27 126488.0 28 157290.0 29 194630.0 30 240448.0 31 299884.0 32 384017.0 33 508279.0 34 724329.0 35 1023133.0 36 1620307.0 37 2356633.0 38 1844008.0 39 283493.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44318238409498 20.158372876268544 13.470619867480762 23.92782487215571 2 15.560024377147922 19.57421183857534 40.42755700284803 24.43820678142871 3 19.171428281454567 24.974107737907964 29.042307690448883 26.812156290188582 4 12.848188118923884 15.934002981247842 37.82458439805895 33.393224501769325 5 13.554863806459752 37.73180401043713 34.294432837429454 14.41889934567366 6 31.918965798049538 36.70006683900788 17.014659635763653 14.366307727178926 7 28.318455933738463 31.68935409693137 21.716592545480378 18.275597423849785 8 26.84168530957866 35.617323240304835 18.995133752925717 18.54585769719078 9 26.272957607875018 15.584792466801552 20.661532914242294 37.48071701108113 10-11 26.287627790878755 25.92582085465175 26.63463632417337 21.151915030296127 12-13 26.008082542066767 23.260061735465413 27.637707446194113 23.0941482762737 14-15 23.274637343416195 24.69242890164046 25.146934902082386 26.885998852860954 16-17 22.46345120987407 27.66040912258884 26.20306328444535 23.673076383091743 18-19 22.098778677824864 27.32877880831317 28.380205214602512 22.192237299259453 20-21 23.23489311485209 26.407344191281556 28.127803535256902 22.229959158609457 22-23 23.421596587199293 26.151102553656063 27.869387009454606 22.557913849690042 24-25 22.91478313317354 26.11400831795455 28.439609045020426 22.53159950385148 26-27 22.883376790168946 26.392373493294052 27.744900746991036 22.979348969545967 28-29 22.335068459823486 26.745028714477105 28.109856235989707 22.810046589709707 30-31 23.06670756045697 26.4131097011367 28.05624455620749 22.46393818219885 32-33 23.215032338477513 26.554538547718483 27.496860794848963 22.73356831895504 34-35 23.18260731602227 26.499940032128855 28.025014119400215 22.29243853244866 36-37 22.949012631118407 26.21317595700271 27.790428099555115 23.04738331232377 38-39 23.03751685064711 26.318033966534653 28.148357334120433 22.496091848697805 40-41 22.80681481356898 25.738783200738443 28.505483413036224 22.948918572656353 42-43 22.81944302612122 25.879444840393575 28.017632948732402 23.283479184752807 44-45 22.862169473948164 26.463515522909148 27.924029493131037 22.750285510011654 46-47 22.840920345878697 26.38962972967055 27.439014752199007 23.330435172251747 48-49 23.38001223636276 26.36120352877795 27.411516303220722 22.847267931638566 50-51 23.06085296504235 26.28955044451323 27.365215812656956 23.284380777787465 52-53 22.772121648828588 26.660139285807155 27.150036023524866 23.417703041839395 54-55 22.660828104674767 26.571290985053608 27.192523918475704 23.57535699179592 56-57 23.305999130557286 26.290299624264456 26.920401528846625 23.48329971633163 58-59 22.62120092036067 26.658453245972698 27.392180124279143 23.32816570938749 60-61 22.759515851891262 26.99328890978519 26.97681277152521 23.270382466798335 62-63 22.952842624903656 26.577567073351084 26.69266415700412 23.776926144741143 64-65 22.48891130864736 26.608309866073203 26.952965306358458 23.949813518920973 66-67 22.729732840337693 26.408248553364714 26.97584314924015 23.886175457057444 68-69 22.464309898360156 26.38647455923449 27.158791931580694 23.990423610824656 70-71 22.41456035187188 26.108601982649628 27.2216876123541 24.25515005312439 72-73 22.77765222646002 26.27877401700813 27.21237780625327 23.731195950278583 74-75 22.863108475975356 26.509014907437496 26.950737709035366 23.677138907551782 76-77 22.701826731015444 26.4078002868071 26.660452174410782 24.22992080776668 78-79 22.574791164099757 26.398774809406227 26.806602916527076 24.219831109966936 80-81 22.320586213841004 26.779220956219792 27.0352707363954 23.864922093543807 82-83 22.86443503012649 26.098452278070212 27.7237041379352 23.313408553868094 84-85 23.011132594803016 26.34839179116981 26.87223508680973 23.768240527217436 86-87 22.12859763378791 26.807882576645213 27.191138876055543 23.872380913511332 88-89 22.6471690766613 26.019299592392848 27.132346991831557 24.201184339114292 90-91 22.565017915843764 26.01613574312976 27.107637460976434 24.311208880050035 92-93 22.7110157627045 26.186447778544412 27.055481043460706 24.047055415290387 94-95 21.945978082370555 26.107988249695186 27.52042709047034 24.42560657746392 96-97 22.11612867679309 26.042993597865326 27.251117709334434 24.589760016007148 98-99 21.671371977321503 26.41135995715938 27.16432889998211 24.752939165537008 100 22.061462416713663 26.034531553012947 26.777660644934105 25.12634538533928 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 695.0 1 520.5 2 517.0 3 715.5 4 607.0 5 549.5 6 923.5 7 1830.5 8 2969.0 9 3643.0 10 3555.0 11 3458.5 12 3964.0 13 4731.5 14 5676.5 15 6791.5 16 7906.0 17 9096.0 18 10315.5 19 11368.0 20 12443.0 21 13591.0 22 15097.5 23 17415.0 24 20466.5 25 24513.5 26 30095.5 27 36937.5 28 46637.0 29 62988.5 30 84854.0 31 106254.0 32 133461.0 33 161796.0 34 184073.0 35 208014.5 36 230673.5 37 265264.0 38 286208.5 39 282081.5 40 275346.0 41 269095.5 42 275797.5 43 293857.5 44 318115.0 45 361444.0 46 403667.0 47 435353.5 48 462994.5 49 467785.5 50 457587.0 51 463953.0 52 465259.5 53 445011.0 54 425927.0 55 384152.0 56 339895.5 57 292398.0 58 244498.5 59 224788.5 60 188735.0 61 141144.0 62 109376.0 63 87746.5 64 64631.5 65 40381.0 66 27248.0 67 19805.0 68 14821.5 69 11080.5 70 8575.5 71 6412.5 72 4687.0 73 3646.5 74 2850.0 75 2033.5 76 1333.0 77 773.5 78 454.5 79 267.5 80 167.0 81 110.5 82 67.0 83 43.0 84 30.0 85 17.5 86 12.0 87 10.5 88 7.5 89 5.0 90 2.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04314813176998682 2 0.0 3 0.0 4 0.030021975196590292 5 0.0 6 0.0 7 8.215930108532437E-4 8 0.0 9 0.011067341146199576 10-11 4.252952056181497E-4 12-13 0.0 14-15 1.6431860217064873E-4 16-17 2.8514110376671396E-4 18-19 0.0 20-21 4.83290006384261E-6 22-23 0.0 24-25 6.041125079803262E-4 26-27 4.687913061927331E-4 28-29 1.498199019791209E-4 30-31 0.002474444832687416 32-33 0.015233301001231906 34-35 0.0262571460468569 36-37 0.031341356914019326 38-39 0.022323165394889016 40-41 0.0014063739185781994 42-43 9.18251012130096E-5 44-45 0.010719372341602909 46-47 0.017780239334876963 48-49 0.0034845209460305218 50-51 0.011415309950796244 52-53 0.010883690943773557 54-55 0.01048256023847462 56-57 4.5912550606504795E-4 58-59 0.008675055614597485 60-61 0.004745907862693443 62-63 0.001362877818003616 64-65 0.03563780507077541 66-67 0.0603629217973942 68-69 0.05952683008634943 70-71 0.05220015358956403 72-73 0.045409928999865165 74-75 0.03514001636419961 76-77 0.03154433871670072 78-79 0.015160807500274267 80-81 0.0010680709141092167 82-83 0.03810741700339898 84-85 0.05174586098356282 86-87 0.05739552115819484 88-89 0.07004322062527095 90-91 0.04878812614449115 92-93 0.038987004815018336 94-95 0.012976336671417408 96-97 0.0036488395482011703 98-99 0.02223617319373985 100 0.03162649801778604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0345755E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64040531966097 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13087592044285 43.615550812770465 2 15.3876522005031 18.35451628311956 3 5.33943338182419 9.553379132079682 4 2.4353741386713574 5.809868029415199 5 1.1893339518885624 3.546617947553401 6 0.6903686710192193 2.470432041574915 7 0.42285288109894054 1.7653382053529059 8 0.299494667769612 1.4289586701485488 9 0.20946689228412374 1.1243421321187415 >10 0.8497689816920635 8.46536416067574 >50 0.0324012398164219 1.3081003271787115 >100 0.012023715173219315 1.2304581256856655 >500 5.634296816079292E-4 0.22187080559132014 >1k 3.0327744428407493E-4 0.2857709894742 >5k 2.1662672615675742E-5 0.07179480485864162 >10k+ 6.498801784702722E-5 0.7476375324023525 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24437 0.2362031577202437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20938 0.20238252307347313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12493 0.12075484099517145 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.66580012768522E-6 2 0.0 0.0 0.0 0.0 9.66580012768522E-6 3 0.0 0.0 0.0 0.0 1.933160025537044E-5 4 0.0 0.0 0.0 0.0 1.933160025537044E-5 5 0.0 2.899740038305566E-5 0.0 0.0 1.933160025537044E-5 6 4.83290006384261E-5 2.899740038305566E-5 0.0 0.0 5.799480076611132E-5 7 4.83290006384261E-5 2.899740038305566E-5 0.0 0.0 5.799480076611132E-5 8 4.83290006384261E-5 2.899740038305566E-5 0.0 0.0 6.766060089379654E-5 9 4.83290006384261E-5 2.899740038305566E-5 0.0 0.0 8.699220114916698E-5 10-11 4.83290006384261E-5 2.899740038305566E-5 0.0 0.0 8.699220114916698E-5 12-13 4.83290006384261E-5 2.899740038305566E-5 0.0 3.866320051074088E-5 9.66580012768522E-5 14-15 4.83290006384261E-5 2.899740038305566E-5 0.0 3.866320051074088E-5 1.0632380140453742E-4 16-17 8.699220114916698E-5 2.899740038305566E-5 0.0 3.866320051074088E-5 1.2565540165990785E-4 18-19 8.699220114916698E-5 2.899740038305566E-5 0.0 3.866320051074088E-5 1.2565540165990785E-4 20-21 8.699220114916698E-5 2.899740038305566E-5 0.0 1.1115670146838002E-4 1.2565540165990785E-4 22-23 8.699220114916698E-5 2.899740038305566E-5 0.0 2.2714630300060267E-4 1.6915150223449133E-4 24-25 8.699220114916698E-5 2.899740038305566E-5 0.0 5.171203068311593E-4 2.2714630300060265E-4 26-27 8.699220114916698E-5 2.899740038305566E-5 0.0 0.0011550631152583838 2.416450031921305E-4 28-29 8.699220114916698E-5 2.899740038305566E-5 0.0 0.0039001503515209857 2.513108033198157E-4 30-31 8.699220114916698E-5 2.899740038305566E-5 0.0 0.01607422561234052 2.561437033836583E-4 32-33 8.699220114916698E-5 2.899740038305566E-5 0.0 0.04575306490439798 3.09305604085927E-4 34-35 8.699220114916698E-5 3.866320051074088E-5 0.0 0.089355489280386 3.3830300446898267E-4 36-37 8.699220114916698E-5 5.316190070226871E-5 0.0 0.164463589172564 3.479688045966679E-4 38-39 8.699220114916698E-5 5.799480076611132E-5 0.0 0.2719086233919129 3.528017046605105E-4 40-41 8.699220114916698E-5 5.799480076611132E-5 0.0 0.38494532298512774 3.576346047243531E-4 42-43 8.699220114916698E-5 5.799480076611132E-5 0.0 0.4956622305476981 3.673004048520384E-4 44-45 8.699220114916698E-5 5.799480076611132E-5 0.0 0.6247199938525512 3.817991050435662E-4 46-47 8.699220114916698E-5 6.282770082995393E-5 0.0 0.7791891456930886 4.0113070529893663E-4 48-49 8.699220114916698E-5 6.766060089379654E-5 0.0 0.9497325231459666 4.5429260600120535E-4 50-51 9.66580012768522E-5 6.766060089379654E-5 0.0 1.1246206777562393 4.5429260600120535E-4 52-53 9.66580012768522E-5 7.249350095763914E-5 0.0 1.292592952375153 4.8812290644810366E-4 54-55 9.66580012768522E-5 1.1115670146838002E-4 0.0 1.4733095844624196 5.364519070865297E-4 56-57 9.66580012768522E-5 1.3532120178759308E-4 0.0 1.6824001728245062 5.509506072780575E-4 58-59 9.66580012768522E-5 1.449870019152783E-4 0.0 1.9182021998394512 5.557835073419001E-4 60-61 1.0632380140453742E-4 1.546528020429635E-4 0.0 2.15891445331926 5.606164074057427E-4 62-63 1.1598960153222264E-4 1.546528020429635E-4 0.0 2.4020673213313093 5.799480076611132E-4 64-65 1.1598960153222264E-4 1.6431860217064873E-4 0.0 2.6481440938819834 5.799480076611132E-4 66-67 1.1598960153222264E-4 1.7398440229833396E-4 0.0 2.9279980049788534 5.992796079164836E-4 68-69 1.1598960153222264E-4 1.8365020242601916E-4 0.0 3.2357135849437766 6.282770082995392E-4 70-71 1.2082250159606525E-4 1.884831024898618E-4 0.0 3.5679368011324453 6.669402088102801E-4 72-73 1.6431860217064873E-4 1.933160025537044E-4 0.0 3.8969799690791054 8.312588109809289E-4 74-75 1.6431860217064873E-4 2.0298180268138962E-4 0.0 4.213157957055817 8.312588109809289E-4 76-77 1.6431860217064873E-4 2.0298180268138962E-4 0.0 4.567491691036565 8.360917110447714E-4 78-79 1.6431860217064873E-4 2.0298180268138962E-4 0.0 4.952987964628971 8.409246111086141E-4 80-81 1.6431860217064873E-4 2.0298180268138962E-4 0.0 5.362890383543782 8.409246111086141E-4 82-83 1.6431860217064873E-4 2.0298180268138962E-4 0.0 5.774450487180491 8.409246111086141E-4 84-85 1.6431860217064873E-4 2.0298180268138962E-4 0.0 6.174488957065 8.409246111086141E-4 86-87 1.6431860217064873E-4 2.0298180268138962E-4 0.0 6.594777278217007 8.409246111086141E-4 88 1.6431860217064873E-4 2.0298180268138962E-4 0.0 6.934834625409166 8.409246111086141E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16355 0.0 47.46178 1 GTATCAA 28510 0.0 38.704666 1 CAACGCA 32670 0.0 32.316494 5 ATCAACG 33370 0.0 31.676397 3 TATCAAC 36325 0.0 30.17086 2 GTGGTAT 5680 0.0 29.38512 1 AACGCAG 37305 0.0 29.14517 6 TCAACGC 37915 0.0 28.189148 4 CTGTGCG 11365 0.0 27.286045 9 TAGTACT 11405 0.0 27.2401 4 TAGGCAT 9905 0.0 26.991566 5 TACCTGG 6570 0.0 26.9675 2 GTACATG 41160 0.0 26.455173 1 GGGCACC 10140 0.0 26.009274 94 ACGCAGA 40620 0.0 25.945356 7 TACATGG 41720 0.0 25.64977 2 CTAGGCA 10970 0.0 25.363995 4 CGCAGAG 41565 0.0 25.230886 8 GCATCGT 9385 0.0 25.03236 8 CTAGTAC 12450 0.0 24.991436 3 >>END_MODULE