FastQCFastQC Report
Mon 6 Jun 2016
ERR1211236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997151
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420900.32384020159494953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312170.24018340634805274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195720.15058684784073065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA235350.048.2284051
GTATCAA440500.042.497041
CAACGCA502000.035.3315665
ATCAACG503550.035.213343
GTACTAG116750.034.01931
TATCAAC549250.033.8336222
GGGCACC123850.031.8761794
AACGCAG571200.031.8497416
GTGGTAT69300.029.878531
CTGTGCG152950.029.656919
TCAACGC605900.029.6529464
TAGGCAT150050.029.1671165
GCATCGT125550.029.0534738
ACGCAGA613750.028.8146787
TCTAACG59100.028.744262
CGCAGAG617700.028.6053858
TAACGCC59950.028.5423894
CTAACGC59700.028.3469473
GTACATG646550.028.0729031
TAGTACT164900.027.9657524
Produced by FastQC (version 0.11.5)