FastQCFastQC Report
Mon 6 Jun 2016
ERR1211236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997151
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387510.29814995609422407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310650.23901391928123325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197790.15217950456988613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236800.048.2342221
GTATCAA437250.042.124351
CAACGCA508500.034.6973085
ATCAACG510750.034.5493473
GGGCACC130100.033.56686894
GTACTAG115400.033.0871731
TATCAAC558500.032.8295562
AACGCAG576900.031.1779986
CTGTGCG157600.030.440129
GTGGTAT72500.029.7055051
TCAACGC607350.029.4489354
TAGTACT168100.028.8020574
ACGCAGA629000.028.0203297
TAGGCAT147600.027.9820375
ACTGTGC184250.027.8478688
CGCAGAG633150.027.7921418
GTACATG644000.027.5711961
TCTAACG52750.026.8198432
TACATGG651400.026.8126752
CTAACGC52900.026.3909323
Produced by FastQC (version 0.11.5)