FastQCFastQC Report
Mon 6 Jun 2016
ERR1211235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8738949
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344220.3938917597528032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259810.2973011972034624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168130.19239155646748826No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC113250.12959224272850203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177100.046.157011
GTATCAA324250.041.7557531
GTACTAG92550.040.068791
GCATCGT94400.036.150238
TACCTGG43850.035.844572
CAACGCA367950.035.310445
ATCAACG379500.034.3594443
GGGCACC98700.033.95132494
ACCTGGG46100.033.138583
TAGGCAT120100.032.876855
TATCAAC410900.032.8007162
GTGGTAT55200.032.2262041
AACGCAG426900.031.3267256
CTAGGCA131250.031.0865024
TCTAACG51000.030.265662
CTAACGC51350.030.0251243
CTGTGCG129900.029.7078029
TAACGCC53050.029.3287854
TAGTACT132150.029.2741374
CGCCTAC52500.029.1888647
Produced by FastQC (version 0.11.5)