FastQCFastQC Report
Mon 6 Jun 2016
ERR1211235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8738949
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321740.36816784260899105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258840.29619122391033526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169870.1943826425809328No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC113970.130416140430617No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA93280.10674052451845181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183250.046.209721
GTATCAA331500.040.7063331
GTACTAG92750.037.4622761
TACCTGG42800.035.9083982
GGGCACC100100.035.64198794
CAACGCA381200.033.7613145
GTGGTAT55350.033.6388551
TAGGCAT115250.033.308985
ATCAACG387250.033.124283
ACCTGGG45400.033.0273
TATCAAC430350.031.2673872
CTGTGCG126150.031.2501749
TATTCTC42300.030.9916025
AACGCAG440750.030.1805926
TAGTACT133300.030.1488194
ACTGTGC137200.029.863468
CTAGGCA131450.029.7149314
CCTGGGG46650.028.3132024
GTACTGT142150.028.0965046
TCAACGC465200.027.9982664
Produced by FastQC (version 0.11.5)