##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211235_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8738949 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17622519595892 33.0 31.0 34.0 30.0 34.0 2 32.314066256708905 34.0 31.0 34.0 31.0 34.0 3 32.38912642698796 34.0 31.0 34.0 30.0 34.0 4 35.99673679294844 37.0 35.0 37.0 35.0 37.0 5 35.89356145687542 37.0 35.0 37.0 35.0 37.0 6 35.92222302704822 37.0 35.0 37.0 35.0 37.0 7 35.862630162963534 37.0 35.0 37.0 35.0 37.0 8 35.8539533758579 37.0 35.0 37.0 35.0 37.0 9 37.4496291258823 39.0 37.0 39.0 34.0 39.0 10-11 37.42946623215217 39.0 37.0 39.0 34.0 39.0 12-13 37.316926554898075 39.0 37.0 39.0 34.0 39.0 14-15 38.51690472160897 40.0 38.0 41.0 34.0 41.0 16-17 38.48002837641002 40.0 38.0 41.0 34.0 41.0 18-19 38.51298182424454 40.0 38.0 41.0 34.0 41.0 20-21 38.475634770268144 40.0 38.0 41.0 34.0 41.0 22-23 38.33775697741227 40.0 38.0 41.0 34.0 41.0 24-25 38.26353312051598 40.0 38.0 41.0 34.0 41.0 26-27 38.11141065132661 40.0 37.0 41.0 33.0 41.0 28-29 38.09968178095558 40.0 37.0 41.0 33.5 41.0 30-31 38.09883230809563 40.0 37.0 41.0 33.5 41.0 32-33 37.95823096118309 40.0 37.0 41.0 33.0 41.0 34-35 37.81488386074801 40.0 37.0 41.0 33.0 41.0 36-37 37.67730644726271 40.0 36.5 41.0 33.0 41.0 38-39 37.57256187214275 40.0 36.0 41.0 33.0 41.0 40-41 37.45413510251633 40.0 36.0 41.0 32.0 41.0 42-43 37.290406088878655 39.0 36.0 41.0 32.0 41.0 44-45 37.0959756144589 39.0 35.5 41.0 31.0 41.0 46-47 36.805137208147116 39.0 35.0 41.0 31.0 41.0 48-49 36.50562310181694 39.0 35.0 41.0 30.0 41.0 50-51 36.26472233674782 39.0 35.0 40.0 30.0 41.0 52-53 36.029652593235184 38.0 35.0 40.0 29.0 41.0 54-55 35.76339197081938 38.0 35.0 40.0 29.0 41.0 56-57 35.460639031077996 37.5 34.0 40.0 28.0 41.0 58-59 35.1438642678885 37.0 34.0 40.0 28.0 41.0 60-61 34.864880662422905 36.5 34.0 39.5 27.0 41.0 62-63 34.53532392739676 36.0 34.0 39.0 27.0 41.0 64-65 34.15640313268793 35.0 33.0 39.0 26.0 40.5 66-67 33.85993555975667 35.0 33.0 38.5 26.0 40.0 68-69 33.55654610182529 35.0 33.0 38.0 26.0 40.0 70-71 33.29283990557674 35.0 33.0 37.0 26.0 40.0 72-73 33.00159590129202 35.0 32.0 37.0 25.0 39.5 74-75 32.70009282580777 35.0 32.0 36.0 25.0 39.0 76-77 31.74737534227514 34.0 30.5 35.0 23.5 38.0 78-79 32.271672142725635 34.5 32.0 35.0 25.0 38.0 80-81 32.24110702557024 35.0 32.0 35.0 25.0 37.0 82-83 32.02817495559249 34.5 32.0 35.0 24.5 37.0 84-85 31.80900901241099 34.0 31.0 35.0 24.0 36.5 86-87 31.604172710013525 34.0 31.0 35.0 24.0 36.0 88-89 31.413550016140384 34.0 31.0 35.0 24.0 36.0 90-91 31.21236878713905 34.0 31.0 35.0 23.0 35.0 92-93 31.030127650361617 34.0 31.0 35.0 22.5 35.0 94-95 30.852533239408995 34.0 30.5 35.0 20.0 35.0 96-97 30.700594030243224 34.0 30.0 35.0 20.0 35.0 98-99 30.544020968654237 34.0 30.0 35.0 20.0 35.0 100 28.698176290993345 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.32676760959916606 1101 2 -0.3452197613428787 1101 3 -0.3646094020192834 1101 4 -0.22291221825377505 1101 5 -0.3000082854091275 1101 6 -0.3272663849070838 1101 7 -0.2797445786786241 1101 8 -0.3130989807442859 1101 9 -0.4878603212213548 1101 10-11 -0.44355085240601255 1101 12-13 -0.4800273301778262 1101 14-15 -0.7445642964851586 1101 16-17 -0.654072865285201 1101 18-19 -0.7007284567677274 1101 20-21 -0.6863932290315233 1101 22-23 -0.6926282790647988 1101 24-25 -0.7554142374162254 1101 26-27 -0.8689846498272047 1101 28-29 -0.8917512780211609 1101 30-31 -0.6698183724359907 1101 32-33 -0.6275281465312759 1101 34-35 -0.6339037825046674 1101 36-37 -0.6077567043116261 1101 38-39 -0.6455583617082183 1101 40-41 -0.7284167980858456 1101 42-43 -0.707418812712568 1101 44-45 -0.7671138241951638 1101 46-47 -0.7307007839712085 1101 48-49 -0.7875308733285493 1101 50-51 -0.7552459512312097 1101 52-53 -0.8388061349755063 1101 54-55 -0.6761167774557251 1101 56-57 -0.6981260213725164 1101 58-59 -0.7617496488795297 1101 60-61 -0.8590809880161814 1101 62-63 -0.9939719297077332 1101 64-65 -0.854221133229764 1101 66-67 -0.8690271259599385 1101 68-69 -0.8801142610335688 1101 70-71 -0.9767892690170825 1101 72-73 -1.0348470479783174 1101 74-75 -0.9927971884500622 1101 76-77 -0.9686066743445103 1101 78-79 -0.8006681683683041 1101 80-81 -0.7848287048420808 1101 82-83 -0.7868355099243054 1101 84-85 -0.7503538017209692 1101 86-87 -0.8345514145163548 1101 88-89 -0.9161466121441784 1101 90-91 -0.8957591827292042 1101 92-93 -1.0113697248040232 1101 94-95 -0.9406679149750303 1101 96-97 -1.0926108944004582 1101 98-99 -1.1325664434590585 1101 100 -0.9267709321170123 1102 1 -0.389977666705974 1102 2 -0.35200665573704626 1102 3 -0.36546154610736536 1102 4 -0.27086020335940475 1102 5 -0.3095543530514604 1102 6 -0.3353557604689712 1102 7 -0.37042560978842687 1102 8 -0.3524814254564106 1102 9 -0.5050339559773747 1102 10-11 -0.5426228976837137 1102 12-13 -0.4823800254411239 1102 14-15 -0.6426968263739141 1102 16-17 -0.6667211481793132 1102 18-19 -0.7118088027826772 1102 20-21 -0.7521246173885885 1102 22-23 -0.7177501469003502 1102 24-25 -0.7480737807980589 1102 26-27 -0.8156989407619903 1102 28-29 -0.8543327213034146 1102 30-31 -0.7511268661120383 1102 32-33 -0.6821671508054905 1102 34-35 -0.6957272808681765 1102 36-37 -0.7557291845671301 1102 38-39 -0.789761655928082 1102 40-41 -0.7652118481422221 1102 42-43 -0.7361673331120997 1102 44-45 -0.7787621403356368 1102 46-47 -0.7876285323462611 1102 48-49 -0.7709304292011154 1102 50-51 -0.8103528430920548 1102 52-53 -0.8553213509559612 1102 54-55 -0.7917703940553906 1102 56-57 -0.8925577503811439 1102 58-59 -0.9338805737383566 1102 60-61 -0.9128681573110455 1102 62-63 -0.7842852381987981 1102 64-65 -0.8274830388617502 1102 66-67 -0.9209680403063203 1102 68-69 -0.9897453919648811 1102 70-71 -1.0249756113018407 1102 72-73 -1.0508082724170826 1102 74-75 -1.04367138572902 1102 76-77 -0.9783934689995952 1102 78-79 -0.7572900639549474 1102 80-81 -0.7839021342399946 1102 82-83 -0.8073053600079412 1102 84-85 -0.9239150070362108 1102 86-87 -0.8359935784863133 1102 88-89 -0.9769633261891961 1102 90-91 -0.9195114756291005 1102 92-93 -0.9988248216086326 1102 94-95 -1.0174925117049085 1102 96-97 -1.1453329093442903 1102 98-99 -1.139628324417302 1102 100 -1.0630722908765762 1103 1 -0.34042839329590535 1103 2 -0.3599320063730467 1103 3 -0.33310362239465974 1103 4 -0.21246030621141898 1103 5 -0.2018730125775292 1103 6 -0.30924288696164837 1103 7 -0.37959025483454667 1103 8 -0.36656606140914505 1103 9 -0.5626866779755133 1103 10-11 -0.5360769625196653 1103 12-13 -0.5430975417426183 1103 14-15 -0.6467204342706694 1103 16-17 -0.6738386956261451 1103 18-19 -0.5788509116968399 1103 20-21 -0.6395945811582706 1103 22-23 -0.6867541976040243 1103 24-25 -0.8568232233674351 1103 26-27 -0.7196684641018152 1103 28-29 -0.7481068406206504 1103 30-31 -0.7244660767820434 1103 32-33 -0.5853266486413702 1103 34-35 -0.7636231537333344 1103 36-37 -0.7140344687213513 1103 38-39 -0.6905564297680442 1103 40-41 -0.8268485138338164 1103 42-43 -0.7632290938734911 1103 44-45 -0.7612536827817706 1103 46-47 -0.7671983152524504 1103 48-49 -0.8124732621783579 1103 50-51 -0.8584419240035785 1103 52-53 -0.8452780767257693 1103 54-55 -0.8474388029619675 1103 56-57 -0.8936184694530738 1103 58-59 -0.9353103534477043 1103 60-61 -0.9806541079029003 1103 62-63 -0.8897092830191013 1103 64-65 -0.8815272566703243 1103 66-67 -0.9369488210697057 1103 68-69 -0.9088800983156133 1103 70-71 -0.9234890061806666 1103 72-73 -0.908484567413403 1103 74-75 -0.9001866282568187 1103 76-77 -0.8998758780612377 1103 78-79 -0.7237955167746257 1103 80-81 -0.701277960755899 1103 82-83 -0.7627914732567724 1103 84-85 -0.8114052514858976 1103 86-87 -0.787363717851786 1103 88-89 -0.8725511095158147 1103 90-91 -0.8978326057107324 1103 92-93 -1.0816607652290031 1103 94-95 -1.0071348128575295 1103 96-97 -1.0950926277325586 1103 98-99 -1.0678880363032413 1103 100 -1.242452502101834 1104 1 -0.40600757736416426 1104 2 -0.36998055396863805 1104 3 -0.4197786010030029 1104 4 -0.30580417534226 1104 5 -0.33511381705123 1104 6 -0.3683468835864332 1104 7 -0.3959694499312505 1104 8 -0.3442922591254245 1104 9 -0.49649959901601903 1104 10-11 -0.562731301266659 1104 12-13 -0.612449367382915 1104 14-15 -0.7691000086304385 1104 16-17 -0.728084879031087 1104 18-19 -0.749225641556059 1104 20-21 -0.7662811476477671 1104 22-23 -0.7810613965170816 1104 24-25 -0.7513111172455851 1104 26-27 -0.8144052754131508 1104 28-29 -0.9774188821106833 1104 30-31 -0.8891323445225296 1104 32-33 -0.8597775908199381 1104 34-35 -0.9090811579632572 1104 36-37 -0.8003438576141093 1104 38-39 -0.7448600235981431 1104 40-41 -0.871769017666999 1104 42-43 -0.7543429090194493 1104 44-45 -0.871545280934761 1104 46-47 -0.9320109262782594 1104 48-49 -0.9370521375142999 1104 50-51 -0.8786623277792742 1104 52-53 -0.8497289595944011 1104 54-55 -0.9748001803889892 1104 56-57 -0.8874090065992135 1104 58-59 -0.9691731509471921 1104 60-61 -0.9285986495764647 1104 62-63 -0.9735380334034147 1104 64-65 -0.9093802654639092 1104 66-67 -0.9805277820043301 1104 68-69 -0.9300444359180915 1104 70-71 -0.9652046810300803 1104 72-73 -0.955921624332035 1104 74-75 -0.9831701317562676 1104 76-77 -0.8914112266289997 1104 78-79 -0.8100378373387933 1104 80-81 -0.7543653159715049 1104 82-83 -0.7577259149273665 1104 84-85 -0.8233227196116069 1104 86-87 -0.7979888462898792 1104 88-89 -0.8373602501082793 1104 90-91 -0.8989981343210509 1104 92-93 -1.0194452646226004 1104 94-95 -1.0539367403987328 1104 96-97 -0.9747197252608757 1104 98-99 -0.9905255768704464 1104 100 -1.1455169187949252 1105 1 -0.4550451322528559 1105 2 -0.38435794277413393 1105 3 -0.48056857696541755 1105 4 -0.2405506865310585 1105 5 -0.2893485402233438 1105 6 -0.3674738836852569 1105 7 -0.4045704414283975 1105 8 -0.4647463629607458 1105 9 -0.5852393718594584 1105 10-11 -0.5983197164768725 1105 12-13 -0.5813178102612397 1105 14-15 -0.8536026367538625 1105 16-17 -0.7377105482759703 1105 18-19 -0.7575569599304544 1105 20-21 -0.8583610460402085 1105 22-23 -0.8321847118735093 1105 24-25 -0.7783626359468983 1105 26-27 -0.8936707543691682 1105 28-29 -0.8809638630904928 1105 30-31 -0.8268494867336784 1105 32-33 -0.8473003895053708 1105 34-35 -0.854723858031349 1105 36-37 -0.7892189460962555 1105 38-39 -0.8351208261053316 1105 40-41 -0.884277399885292 1105 42-43 -0.8769263889809693 1105 44-45 -0.8808785494972753 1105 46-47 -0.8852997251493022 1105 48-49 -0.9662428591499577 1105 50-51 -0.9898827555453096 1105 52-53 -1.069116547351392 1105 54-55 -0.9255914493696835 1105 56-57 -0.9961665272140863 1105 58-59 -1.0181435287289418 1105 60-61 -0.9725825435523703 1105 62-63 -0.9976576887398068 1105 64-65 -0.9919579919191932 1105 66-67 -0.875575934018272 1105 68-69 -0.9963420844103155 1105 70-71 -0.9955309549326827 1105 72-73 -0.9420295588533776 1105 74-75 -1.042558610302791 1105 76-77 -0.9669528930476794 1105 78-79 -0.9614366254333717 1105 80-81 -0.88430605034441 1105 82-83 -0.8426713380448696 1105 84-85 -0.8454108172573136 1105 86-87 -0.8496052679360133 1105 88-89 -0.8649346253113102 1105 90-91 -0.8730349795866559 1105 92-93 -0.9560272715112426 1105 94-95 -0.9835610973314246 1105 96-97 -1.0034508269680416 1105 98-99 -1.0639768065033657 1105 100 -0.9985718167409878 1106 1 -0.4180344324202494 1106 2 -0.4021280850171749 1106 3 -0.45370344480881286 1106 4 -0.2802285355853016 1106 5 -0.33434766771377156 1106 6 -0.361081060202487 1106 7 -0.44713567304740565 1106 8 -0.4358120027346857 1106 9 -0.557423994391776 1106 10-11 -0.59651451631985 1106 12-13 -0.625651887472003 1106 14-15 -0.7800260386532898 1106 16-17 -0.7292191594350399 1106 18-19 -0.7200817440596623 1106 20-21 -0.7148873853147251 1106 22-23 -0.7720192667817258 1106 24-25 -0.7886234621980961 1106 26-27 -0.7756018944261953 1106 28-29 -0.7083968658539987 1106 30-31 -0.7436488744643839 1106 32-33 -0.8282728481475559 1106 34-35 -0.7876474638852642 1106 36-37 -0.7779539498765189 1106 38-39 -0.74218469183797 1106 40-41 -0.8149051649843244 1106 42-43 -0.714080026048471 1106 44-45 -0.7760992430753575 1106 46-47 -0.86154927997881 1106 48-49 -0.9168321628098539 1106 50-51 -0.9953305985959062 1106 52-53 -0.9058385873242614 1106 54-55 -0.8947535856272779 1106 56-57 -0.9553099294184904 1106 58-59 -0.9343518434930047 1106 60-61 -0.8901471141792854 1106 62-63 -1.0096542802341517 1106 64-65 -0.9814902520841997 1106 66-67 -0.9519222811484553 1106 68-69 -0.934438037958131 1106 70-71 -0.8896345795550928 1106 72-73 -1.0496462507053828 1106 74-75 -1.0595449004659017 1106 76-77 -1.0448189839810063 1106 78-79 -0.814707225082941 1106 80-81 -0.785793602295314 1106 82-83 -0.8308336767197595 1106 84-85 -0.8158991649812144 1106 86-87 -0.876620037942736 1106 88-89 -0.8585292045489545 1106 90-91 -0.9008397350650803 1106 92-93 -0.8737304737012828 1106 94-95 -0.8641474720277387 1106 96-97 -0.9455236471452366 1106 98-99 -0.9484126454456003 1106 100 -0.9924553955575597 1107 1 -0.4287844551474471 1107 2 -0.4036243970435862 1107 3 -0.44432183650422274 1107 4 -0.23305703270751366 1107 5 -0.24988586893925913 1107 6 -0.33287984556498174 1107 7 -0.3570215682272746 1107 8 -0.3568019767274606 1107 9 -0.5461477913005979 1107 10-11 -0.5718714504228544 1107 12-13 -0.5604088486192254 1107 14-15 -0.7467483926704332 1107 16-17 -0.7149546364686898 1107 18-19 -0.6801683481111738 1107 20-21 -0.6503448710034405 1107 22-23 -0.6952516602254164 1107 24-25 -0.7259500816424307 1107 26-27 -0.813361773962896 1107 28-29 -0.7756992074522628 1107 30-31 -0.8107028228985413 1107 32-33 -0.8071137606594831 1107 34-35 -0.7742165808981412 1107 36-37 -0.729059662421065 1107 38-39 -0.8428117969591611 1107 40-41 -0.8467437666311 1107 42-43 -0.7245283280639541 1107 44-45 -0.7425364720906131 1107 46-47 -0.7622469621927124 1107 48-49 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0.9884718342165542 2311 38-39 0.9433786526193231 2311 40-41 0.9519865209687737 2311 42-43 0.9473801111651596 2311 44-45 0.9924753485695774 2311 46-47 0.638060731959861 2311 48-49 0.9180305472993666 2311 50-51 1.0291985717533336 2311 52-53 0.9131969132330937 2311 54-55 0.9237912353563686 2311 56-57 1.0243306449582263 2311 58-59 1.0789443289408638 2311 60-61 1.0337951628094686 2311 62-63 1.032757455359807 2311 64-65 1.0938787566682464 2311 66-67 1.0266109098146785 2311 68-69 1.1000150018854455 2311 70-71 1.105128472381601 2311 72-73 1.1481228959576413 2311 74-75 1.1050635146465524 2311 76-77 1.0946993746698759 2311 78-79 0.8073329451956326 2311 80-81 0.824931707687341 2311 82-83 0.8671039004790764 2311 84-85 0.9362010463973611 2311 86-87 0.9682340514285634 2311 88-89 1.008361762265249 2311 90-91 1.0651637817654738 2311 92-93 1.1593467484475788 2311 94-95 1.2176131853694727 2311 96-97 1.2507224453725136 2311 98-99 1.2648762245912302 2311 100 1.4001142296368947 2312 1 0.26889013804841966 2312 2 0.3265722346684683 2312 3 0.42160357967267004 2312 4 0.17010373027918746 2312 5 0.32846906943913723 2312 6 0.39484566971679413 2312 7 0.3127714946266025 2312 8 0.38566595135119996 2312 9 0.5740066497340948 2312 10-11 0.5993903785890424 2312 12-13 0.629869430916763 2312 14-15 0.820440354578551 2312 16-17 0.8094895883056097 2312 18-19 0.7718243457390699 2312 20-21 0.8273726388559552 2312 22-23 0.9015708059893086 2312 24-25 0.9079391655697933 2312 26-27 0.9506123687243075 2312 28-29 0.9001500826558981 2312 30-31 0.9666358039351906 2312 32-33 0.8371376983798413 2312 34-35 0.9615684171187837 2312 36-37 0.999133742596257 2312 38-39 0.7975312772738832 2312 40-41 0.9415705332179982 2312 42-43 1.0018123441393598 2312 44-45 0.7458263784431125 2312 46-47 0.7169128133146359 2312 48-49 1.0242638844103737 2312 50-51 0.8521941213091537 2312 52-53 1.0158014296220372 2312 54-55 0.8618114171200304 2312 56-57 0.9144705503319699 2312 58-59 1.057852321254252 2312 60-61 1.0350101385962276 2312 62-63 1.0995040404727092 2312 64-65 1.0554865991509743 2312 66-67 1.0595674564891624 2312 68-69 1.1156725130555927 2312 70-71 1.0520893224967764 2312 72-73 1.0822405074084003 2312 74-75 1.1083520668218227 2312 76-77 1.1150955317914502 2312 78-79 1.0071375731339955 2312 80-81 0.9643523884064251 2312 82-83 0.9317411339756525 2312 84-85 1.0026330167992974 2312 86-87 1.0093167133721757 2312 88-89 1.025073129313487 2312 90-91 1.1415999793083778 2312 92-93 1.1939555783012885 2312 94-95 1.1642621554959405 2312 96-97 0.8030507523804147 2312 98-99 0.6144247253527446 2312 100 0.8959596074672405 2313 1 0.045616812492475844 2313 2 0.21394949712604472 2313 3 0.2940410437581633 2313 4 0.1873596471625163 2313 5 0.32478415831956653 2313 6 0.22500276330089264 2313 7 0.22984227553133962 2313 8 0.36887690544313045 2313 9 0.5843779882864766 2313 10-11 0.3507279859205923 2313 12-13 0.3975229492674188 2313 14-15 0.44231267571411337 2313 16-17 0.6945116101048399 2313 18-19 0.6750265667699225 2313 20-21 0.6397362251778063 2313 22-23 0.6179161155220143 2313 24-25 0.5194868270564186 2313 26-27 0.703917810039556 2313 28-29 0.5562922574730749 2313 30-31 0.7573741568454508 2313 32-33 0.874201680935208 2313 34-35 0.8887429500122295 2313 36-37 0.9162096265876656 2313 38-39 0.8934568882000704 2313 40-41 0.9210887371936991 2313 42-43 0.9901841531225841 2313 44-45 0.8324399032069891 2313 46-47 0.8800255723651347 2313 48-49 1.0000548230127748 2313 50-51 0.9275224726156566 2313 52-53 0.9551929659729694 2313 54-55 0.8911341901746539 2313 56-57 0.5642655596588781 2313 58-59 0.6285990612536239 2313 60-61 0.5859230061453644 2313 62-63 0.897285258446118 2313 64-65 0.9604687048884841 2313 66-67 0.9182943560881398 2313 68-69 0.9441865948206996 2313 70-71 0.9340528655175078 2313 72-73 0.9884403707767788 2313 74-75 1.0482260975141813 2313 76-77 1.0363679420694147 2313 78-79 0.9086698101883499 2313 80-81 0.8010467090078208 2313 82-83 0.9299794583204637 2313 84-85 0.9120358568741587 2313 86-87 0.9046424635156747 2313 88-89 0.9041882440689797 2313 90-91 0.7467532956523613 2313 92-93 0.9730987850709383 2313 94-95 1.089725561061087 2313 96-97 1.1499111317924076 2313 98-99 1.2272746390213563 2313 100 1.2996860443286984 2314 1 -0.3926055511706572 2314 2 0.0032547705391294812 2314 3 0.27706440482543115 2314 4 0.21823584806574559 2314 5 0.3367929426917158 2314 6 0.39269321649840805 2314 7 0.4305859267433192 2314 8 0.4475993819574029 2314 9 0.4280317180154469 2314 10-11 0.5637990760113141 2314 12-13 0.4700046600687102 2314 14-15 0.7613422514174459 2314 16-17 0.8002684827452242 2314 18-19 0.833424144163736 2314 20-21 0.8652604836263933 2314 22-23 0.9028764117461776 2314 24-25 0.854332529728218 2314 26-27 0.7407387546840027 2314 28-29 0.7823367921762028 2314 30-31 0.800096330913938 2314 32-33 0.8213804512208682 2314 34-35 0.8674641812544195 2314 36-37 0.793683783221006 2314 38-39 0.5240292207766046 2314 40-41 0.5306964316447562 2314 42-43 0.6753480070144846 2314 44-45 0.7629225410909655 2314 46-47 0.7503584270980284 2314 48-49 0.7619392183942608 2314 50-51 0.7080216276866977 2314 52-53 0.680518099360782 2314 54-55 0.668947677745507 2314 56-57 0.6564478872191231 2314 58-59 0.8229674942259635 2314 60-61 0.8619305552146344 2314 62-63 0.8352468301922045 2314 64-65 0.843970623401681 2314 66-67 0.4900516458727253 2314 68-69 0.5469023577125043 2314 70-71 0.6909361288773468 2314 72-73 0.8427660164110762 2314 74-75 0.9393297175692581 2314 76-77 0.8316201882519714 2314 78-79 0.7140217701943854 2314 80-81 0.7639593911000162 2314 82-83 0.8204467346895434 2314 84-85 0.7695206980998996 2314 86-87 0.823347499726804 2314 88-89 0.8185740729394091 2314 90-91 0.937750797289052 2314 92-93 0.9023837809965123 2314 94-95 0.9001120972881829 2314 96-97 0.8944648150022623 2314 98-99 1.0226778755405341 2314 100 1.0483418196298757 2315 1 0.3403026129015174 2315 2 0.4084493470615058 2315 3 0.4213620193349925 2315 4 0.2829825926332319 2315 5 0.34130352399590436 2315 6 0.39895553112695126 2315 7 0.37903806172941046 2315 8 0.22808035605643084 2315 9 0.4619709434243475 2315 10-11 0.5124044033624031 2315 12-13 0.35720669464524946 2315 14-15 0.5450263326617559 2315 16-17 0.7119204553089773 2315 18-19 0.6939492595167636 2315 20-21 0.4600619077128698 2315 22-23 0.8083237471753506 2315 24-25 0.8551126985406938 2315 26-27 0.7793738142408131 2315 28-29 0.7805339947301775 2315 30-31 0.8299628822099336 2315 32-33 0.7471947514386414 2315 34-35 0.8222509466574266 2315 36-37 0.8091177996320482 2315 38-39 0.8812165305359585 2315 40-41 0.8269350068807384 2315 42-43 0.9160190154515533 2315 44-45 0.9686632723778104 2315 46-47 0.9391539602643064 2315 48-49 0.8080511919884543 2315 50-51 0.8880263684029117 2315 52-53 0.9619662072736688 2315 54-55 0.7505519997855785 2315 56-57 0.7445808493566801 2315 58-59 0.738844039346958 2315 60-61 0.8534380049432642 2315 62-63 0.9478766382086192 2315 64-65 0.9988440265957479 2315 66-67 0.9920010223114417 2315 68-69 1.030530575565777 2315 70-71 0.9642609505652757 2315 72-73 1.027099614462287 2315 74-75 0.46918049348904134 2315 76-77 0.4644174620160193 2315 78-79 0.7216079747834314 2315 80-81 0.7929080167772042 2315 82-83 0.7515486613165017 2315 84-85 0.7997360329000465 2315 86-87 0.8276559810787809 2315 88-89 0.7976616356354178 2315 90-91 0.8759919900089166 2315 92-93 0.9059397625264332 2315 94-95 0.9171717747129762 2315 96-97 1.0798480963918706 2315 98-99 1.0550475229011447 2315 100 1.0546908922926157 2316 1 0.2562933689503524 2316 2 0.08290411972702572 2316 3 0.21610817864969079 2316 4 0.06732833601316202 2316 5 0.3003817549744312 2316 6 0.360507507587684 2316 7 0.39147778571097547 2316 8 0.39965041307042526 2316 9 0.6159288128680842 2316 10-11 0.5870727307732864 2316 12-13 0.35643266846376065 2316 14-15 0.4813112270563451 2316 16-17 0.7666918910471594 2316 18-19 0.717816597443452 2316 20-21 0.6986199929924766 2316 22-23 0.756432833069276 2316 24-25 0.4490900770408288 2316 26-27 0.6966290430896933 2316 28-29 0.8352675049872786 2316 30-31 0.5089702782560224 2316 32-33 0.6293157918710293 2316 34-35 0.7577120595432376 2316 36-37 0.7718018070951871 2316 38-39 0.7412699440627861 2316 40-41 0.740007595709244 2316 42-43 0.7330585964813494 2316 44-45 0.4713945673879678 2316 46-47 0.6569093987441832 2316 48-49 0.7431665322455316 2316 50-51 0.8345448888340243 2316 52-53 0.8388780875408202 2316 54-55 0.7147373087592115 2316 56-57 0.8154578881771002 2316 58-59 0.6711667005933606 2316 60-61 0.8295063004078855 2316 62-63 0.829759061240317 2316 64-65 0.8949042732166888 2316 66-67 0.8443138285306162 2316 68-69 0.8146795805125819 2316 70-71 0.8066950086409079 2316 72-73 0.8485829493009192 2316 74-75 0.8326187124692765 2316 76-77 0.7410087827521785 2316 78-79 0.5058150181365377 2316 80-81 0.5201359389298403 2316 82-83 0.3978387130867098 2316 84-85 0.563830028580913 2316 86-87 0.6066786960103805 2316 88-89 0.6213411409695873 2316 90-91 0.5181461394912539 2316 92-93 0.33840902639931514 2316 94-95 0.32293055784242597 2316 96-97 0.6034264318316573 2316 98-99 0.7784563945913199 2316 100 0.4065488726316495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 5.0 12 39.0 13 181.0 14 811.0 15 2267.0 16 5539.0 17 11572.0 18 19927.0 19 29648.0 20 44080.0 21 63347.0 22 77743.0 23 76180.0 24 73006.0 25 75589.0 26 87992.0 27 107066.0 28 133024.0 29 163881.0 30 202989.0 31 253360.0 32 324696.0 33 433072.0 34 641212.0 35 892764.0 36 1406013.0 37 1969098.0 38 1404134.0 39 239694.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.885794447773264 21.876320095723468 13.555890549024735 22.681994907478533 2 15.463324022144997 20.11428376570226 41.25433161356131 23.168060598591435 3 18.71096856155128 27.27427520174337 28.91555952552189 25.099196711183463 4 12.279498579271305 16.605995793333484 39.78277308933111 31.331732538064106 5 12.776708045784451 38.96333529352328 34.33192023434397 13.928036426348294 6 29.483980281839383 38.44326131208684 17.842065447458268 14.230692958615505 7 27.225300739511667 32.26512565976742 23.01216117635279 17.497412424368125 8 27.02797556090555 35.00339686156767 19.358998433335632 18.609629144191135 9 26.84305869527227 15.102871253373642 21.842796048185612 36.21127400316848 10-11 26.30143894474639 26.22692159108282 26.50279142556092 20.968848038609874 12-13 26.103213326911508 23.955998598916185 27.64926308644209 22.29152498773022 14-15 23.578015061899688 25.000642240546977 25.513502070474814 25.90784062707852 16-17 21.672226370647785 27.664308119380138 26.849654096450887 23.813811413521194 18-19 21.108540689992623 27.56657737484092 29.616578012789525 21.70830392237693 20-21 22.762349497768525 26.707303515977927 28.975551234796725 21.554795751456826 22-23 23.124691538993993 26.416494706628907 28.676818001798615 21.781995752578485 24-25 22.41000455377653 26.356044447467962 29.639209188844752 21.594741809910754 26-27 22.280966551301336 26.713112226302954 28.56341269300902 22.44250852938669 28-29 21.494434331334766 27.16504144900086 29.153276395788406 22.18724782387596 30-31 22.595949847186066 26.653929883649024 29.052744723147477 21.69737554601743 32-33 22.926782958529824 26.94824502412345 28.10133634622771 22.023635671119017 34-35 22.820447372110923 26.799907286322156 29.006258173254395 21.373387168312526 36-37 22.362438642796164 26.41270662162159 28.674318102575764 22.550536633006484 38-39 22.63134135934278 26.429764557977357 29.271947760128047 21.666946322551816 40-41 22.11992738400886 25.524827561623837 29.93954636919186 22.41569868517544 42-43 22.28097647752877 25.736732532195116 29.034219991059558 22.94807099921655 44-45 22.2041153444545 26.810577148002523 28.85861895011314 22.126688557429837 46-47 22.343099059670198 26.5422335808528 28.09991805305035 23.014749306426655 48-49 23.266647662988106 26.547803272790482 28.038123469516318 22.14742559470509 50-51 22.769249027250936 26.262453731511705 28.003848938546362 22.964448302691 52-53 22.095960838644807 26.95738682595861 27.709944447254646 23.236707888141936 54-55 22.139034330369228 26.897590028805624 27.613304592368742 23.350071048456403 56-57 23.237629581348344 26.283243268727276 27.204261811996734 23.27486533792765 58-59 22.001872818299717 26.880269804853246 28.10668255100397 23.01117482584306 60-61 22.294916767307964 27.664581631939004 27.1988976194946 22.841603981258434 62-63 22.758046005882473 26.723424015478486 26.84827469644423 23.67025528219481 64-65 21.771790807333826 26.904238267203805 27.302672415791413 24.021298509670956 66-67 22.3052293090732 26.533716911528764 27.35851956876822 23.802534210629815 68-69 21.90518151293516 26.611504548750787 27.561277507660925 23.922036430653126 70-71 21.88388509203916 26.14245525239529 27.81325613084946 24.160403524716084 72-73 22.555150045054674 26.36716690652704 27.858224146396832 23.219458902021454 74-75 22.725100120589428 26.82752163343642 27.32219641601744 23.125181829956716 76-77 22.415663544613047 26.60101519158795 27.032914315501728 23.950406948297275 78-79 22.25557794148346 26.669393950214427 27.112912285616975 23.96211582268514 80-81 21.741521060538503 27.258126318431035 27.63837686302541 23.361975758005048 82-83 22.905778153914184 26.039660806413846 28.724942409518107 22.32961863015386 84-85 23.07635943479588 26.627111964848883 27.217753430629827 23.078775169725414 86-87 21.565203233620068 27.43342871044312 27.731324507940386 23.270043547996426 88-89 22.58783414683804 26.15933653605188 27.579576001928803 23.673253315181274 90-91 22.37813656489795 26.09346655714151 27.79708460818519 23.731312269775348 92-93 22.821453500501832 26.445557336602754 27.42329787170107 23.309691291194348 94-95 21.481988856443596 26.41039462518247 28.17623116155868 23.93138535681526 96-97 21.878471303963526 26.411697465420993 27.70229731017893 24.007533920436554 98-99 21.163664016400286 26.97725130954322 27.573611319915297 24.2854733541412 100 21.874685926125018 26.42814184337412 26.76504652814869 24.932125702352174 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2025.0 1 1382.0 2 1181.0 3 1574.0 4 1174.5 5 977.5 6 1506.5 7 2967.5 8 4544.0 9 5021.5 10 4344.0 11 3702.0 12 4092.5 13 4949.5 14 6113.5 15 7450.5 16 8800.0 17 10299.0 18 11658.5 19 12735.0 20 13772.0 21 14550.0 22 15455.0 23 16806.5 24 18137.0 25 19696.0 26 22740.5 27 26273.5 28 31746.5 29 45954.5 30 68013.0 31 89673.0 32 121857.0 33 158328.0 34 185409.0 35 214040.0 36 240470.0 37 280498.0 38 296275.5 39 274629.5 40 247820.5 41 221740.5 42 215073.0 43 225635.0 44 250110.0 45 303308.5 46 350224.0 47 381453.0 48 409740.0 49 408530.0 50 389796.5 51 394665.0 52 396915.0 53 373618.5 54 353172.0 55 307661.5 56 265312.5 57 217756.0 58 169819.0 59 163030.0 60 134929.0 61 89528.0 62 65047.5 63 54904.0 64 39472.5 65 18732.0 66 10175.5 67 6519.5 68 5048.0 69 4027.5 70 3179.5 71 2606.0 72 2214.0 73 2141.5 74 1722.0 75 1277.0 76 859.5 77 479.0 78 299.5 79 202.0 80 147.0 81 92.5 82 63.5 83 40.5 84 19.5 85 12.0 86 8.0 87 5.5 88 3.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04333473052651984 2 0.0 3 0.0 4 0.03765899080083887 5 0.0 6 0.0 7 8.467837493959515E-4 8 0.0 9 0.0113057073568 10-11 5.149360638218623E-4 12-13 0.0 14-15 1.3159477186558703E-4 16-17 2.8035407919190284E-4 18-19 5.721511820242915E-6 20-21 5.721511820242915E-6 22-23 0.0 24-25 5.549866465635628E-4 26-27 5.034930401813765E-4 28-29 1.945314018882591E-4 30-31 0.002483136129985425 32-33 0.015940131931196762 34-35 0.027165738122513362 36-37 0.03216633945340566 38-39 0.02312635077742186 40-41 0.0011729099231497975 42-43 8.582267730364373E-5 44-45 0.011637555042374088 46-47 0.018182964564731983 48-49 0.0033184768557408904 50-51 0.01093380908848421 52-53 0.01083654338754008 54-55 0.010281556740976519 56-57 4.2339187469797574E-4 58-59 0.008942722975039676 60-61 0.0046401460862170035 62-63 0.0012587326004534413 64-65 0.03577661341197895 66-67 0.05977263398607774 68-69 0.05924625489861538 70-71 0.051665251736793524 72-73 0.045840752703786236 74-75 0.0355706389864502 76-77 0.03164568187776356 78-79 0.015070462134519836 80-81 0.001207238994071255 82-83 0.03830552163652631 84-85 0.05203142849328907 86-87 0.05747258623434008 88-89 0.06929322965496194 90-91 0.04890748303943643 92-93 0.03949559609513684 94-95 0.013319679517525504 96-97 0.00386774199048421 98-99 0.023212173454725506 100 0.03372259066851174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8738949.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.738558397938 #Duplication Level Percentage of deduplicated Percentage of total 1 75.54089015361188 45.882447680273756 2 14.659469353922203 17.807900708719725 3 4.830137006213847 8.801266758658837 4 2.0670024126551034 5.021869869989231 5 1.0203719244277711 3.0987959859736276 6 0.5900066053559514 2.150169039274897 7 0.3205860897397235 1.3630355853255953 8 0.22094939583875883 1.0736118225713258 9 0.1414750670144729 0.773369245773968 >10 0.5455588227803143 5.604585035695247 >50 0.033321465089645744 1.415565367552551 >100 0.026526253956287008 3.1724690759780545 >500 0.0023857399887312314 0.9818091208363251 >1k 0.001167435243686655 1.2788934561274559 >5k 5.075805387989599E-5 0.24172034280347893 >10k+ 1.0151610775979198E-4 1.332490904446038 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32174 0.36816784260899105 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25884 0.29619122391033526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16987 0.1943826425809328 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 11397 0.130416140430617 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 9328 0.10674052451845181 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.144302364048583E-5 2 0.0 0.0 0.0 0.0 1.144302364048583E-5 3 0.0 0.0 0.0 0.0 1.144302364048583E-5 4 0.0 0.0 0.0 0.0 1.144302364048583E-5 5 0.0 0.0 0.0 0.0 1.144302364048583E-5 6 0.0 0.0 0.0 0.0 3.432907092145749E-5 7 0.0 0.0 0.0 0.0 3.432907092145749E-5 8 0.0 0.0 0.0 0.0 3.432907092145749E-5 9 0.0 0.0 0.0 0.0 5.721511820242915E-5 10-11 0.0 0.0 0.0 5.721511820242915E-6 5.721511820242915E-5 12-13 0.0 5.721511820242915E-6 0.0 1.7164535460728746E-5 5.721511820242915E-5 14-15 0.0 2.8607559101214574E-5 0.0 2.8607559101214577E-5 9.726570094412956E-5 16-17 0.0 4.577209456194332E-5 0.0 5.1493606382186234E-5 1.0298721276437247E-4 18-19 0.0 4.577209456194332E-5 0.0 1.144302364048583E-4 1.0298721276437247E-4 20-21 0.0 4.577209456194332E-5 0.0 1.9453140188825912E-4 1.0298721276437247E-4 22-23 0.0 4.577209456194332E-5 0.0 5.778726938445343E-4 1.2587326004534412E-4 24-25 0.0 6.293663002267206E-5 0.0 0.0013216692304761132 1.4875930732631578E-4 26-27 0.0 6.865814184291498E-5 0.0 0.002477414618165182 1.4875930732631578E-4 28-29 0.0 6.865814184291498E-5 0.0 0.006270776954986235 1.4875930732631578E-4 30-31 0.0 6.865814184291498E-5 0.0 0.020088228000872874 1.5448081914655871E-4 32-33 0.0 6.865814184291498E-5 0.0 0.05128191044483724 1.6020233096680162E-4 34-35 0.0 9.154418912388664E-5 0.0 0.09688808116399353 1.7164535460728744E-4 36-37 0.0 9.154418912388664E-5 0.0 0.1724005941675595 1.7164535460728744E-4 38-39 0.0 9.726570094412956E-5 0.0 0.2843305299069716 1.7164535460728744E-4 40-41 0.0 1.0870872458461537E-4 0.0 0.40416759498195953 1.7164535460728744E-4 42-43 0.0 1.2587326004534412E-4 0.0 0.5217046123052097 1.7164535460728744E-4 44-45 0.0 1.2587326004534412E-4 0.0 0.6569096581293701 1.7736686642753034E-4 46-47 0.0 1.2587326004534412E-4 0.0 0.8139537145713975 1.8308837824777328E-4 48-49 0.0 1.3159477186558703E-4 0.0 0.9880593192613893 1.8308837824777328E-4 50-51 0.0 1.3731628368582997E-4 0.0 1.1715195957774784 1.8308837824777328E-4 52-53 0.0 1.3731628368582997E-4 0.0 1.3477307168173198 2.0025291370850203E-4 54-55 0.0 1.3731628368582997E-4 0.0 1.5352818742848826 2.1741744916923075E-4 56-57 0.0 1.3731628368582997E-4 0.0 1.7497126942839465 2.1741744916923075E-4 58-59 0.0 1.4303779550607287E-4 0.0 2.0012475184372858 2.1741744916923075E-4 60-61 0.0 1.8308837824777328E-4 0.0 2.256947603195762 2.288604728097166E-4 62-63 0.0 1.8308837824777328E-4 0.0 2.5174365933477816 2.288604728097166E-4 64-65 0.0 1.8308837824777328E-4 0.0 2.777313381735035 2.288604728097166E-4 66-67 0.0 2.0597442552874493E-4 0.0 3.065282793159681 2.288604728097166E-4 68-69 0.0 2.0597442552874493E-4 0.0 3.382574952663072 2.288604728097166E-4 70-71 0.0 2.0597442552874493E-4 0.0 3.7278453049674507 2.288604728097166E-4 72-73 0.0 2.0597442552874493E-4 0.0 4.078093372555442 2.4030349645020243E-4 74-75 0.0 2.0597442552874493E-4 0.0 4.407303441180398 2.4030349645020243E-4 76-77 0.0 2.1741744916923075E-4 0.0 4.776392447192448 2.4030349645020243E-4 78-79 0.0 2.1741744916923075E-4 0.0 5.171079497088265 2.4030349645020243E-4 80-81 0.0 2.1741744916923075E-4 0.0 5.588652594265054 2.4602500827044534E-4 82-83 0.0 2.1741744916923075E-4 0.0 6.018155043587049 2.7463256737165993E-4 84-85 0.0 2.1741744916923075E-4 0.0 6.425686887519312 2.7463256737165993E-4 86-87 0.0 2.1741744916923075E-4 0.0 6.855120678699464 2.8035407919190284E-4 88 0.0 2.1741744916923075E-4 0.0 7.201827130470724 3.089616382931174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18325 0.0 46.20972 1 GTATCAA 33150 0.0 40.706333 1 GTACTAG 9275 0.0 37.462276 1 TACCTGG 4280 0.0 35.908398 2 GGGCACC 10010 0.0 35.641987 94 CAACGCA 38120 0.0 33.761314 5 GTGGTAT 5535 0.0 33.638855 1 TAGGCAT 11525 0.0 33.30898 5 ATCAACG 38725 0.0 33.12428 3 ACCTGGG 4540 0.0 33.027 3 TATCAAC 43035 0.0 31.267387 2 CTGTGCG 12615 0.0 31.250174 9 TATTCTC 4230 0.0 30.991602 5 AACGCAG 44075 0.0 30.180592 6 TAGTACT 13330 0.0 30.148819 4 ACTGTGC 13720 0.0 29.86346 8 CTAGGCA 13145 0.0 29.714931 4 CCTGGGG 4665 0.0 28.313202 4 GTACTGT 14215 0.0 28.096504 6 TCAACGC 46520 0.0 27.998266 4 >>END_MODULE