FastQCFastQC Report
Mon 6 Jun 2016
ERR1211234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612263
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360690.37523942072746036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279920.29121134117949127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172680.17964552155928318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188450.047.9461331
GTATCAA344200.040.948171
GTACTAG98400.037.5424921
GCATCGT91000.036.9860888
GGGCACC95150.034.47720394
CAACGCA394250.033.9709665
ATCAACG397650.033.6688583
TATCAAC435350.032.1471062
TAGGCAT123350.031.8987335
GTGGTAT61750.031.3984891
CTGTGCG124050.031.1499
AACGCAG449150.030.4260276
TAGTACT135850.029.5865024
CTAGGCA138150.029.0939254
ACTGTGC139650.028.6117748
TACCTGG42150.028.4696452
TCAACGC476850.028.3822614
ACGCAGA474600.028.2005867
CGCAGAG475500.028.0858428
CATCGTC124200.027.9321129
Produced by FastQC (version 0.11.5)