##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211234_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9612263 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404693983092223 31.0 31.0 34.0 28.0 34.0 2 31.425260211877266 31.0 31.0 34.0 28.0 34.0 3 31.484441384926733 31.0 31.0 34.0 28.0 34.0 4 35.08539539544434 37.0 35.0 37.0 32.0 37.0 5 35.14768145648949 37.0 35.0 37.0 32.0 37.0 6 35.20998416293853 37.0 35.0 37.0 32.0 37.0 7 35.120939366723526 37.0 35.0 37.0 32.0 37.0 8 35.134403209733236 37.0 35.0 37.0 32.0 37.0 9 36.68331806984474 39.0 37.0 39.0 32.0 39.0 10-11 36.63924083225771 39.0 37.0 39.0 32.0 39.0 12-13 36.525431212192174 39.0 37.0 39.0 32.0 39.0 14-15 37.68745523296647 40.0 37.0 41.0 32.0 41.0 16-17 37.67601495090178 40.0 37.0 41.0 32.0 41.0 18-19 37.71498215352618 40.0 37.0 41.0 32.0 41.0 20-21 37.68857390814213 40.0 37.0 41.0 32.0 41.0 22-23 37.57647845257667 40.0 37.0 41.0 32.0 41.0 24-25 37.474430578938595 40.0 37.0 41.0 31.5 41.0 26-27 37.31797132475464 40.0 36.5 41.0 31.0 41.0 28-29 37.20677347259433 40.0 36.0 41.0 31.0 41.0 30-31 37.05452930282911 39.0 36.0 41.0 30.5 41.0 32-33 36.869119790001584 39.0 35.5 41.0 30.0 41.0 34-35 36.7220042772446 39.0 35.0 41.0 30.0 41.0 36-37 36.612041722121006 39.0 35.0 41.0 30.0 41.0 38-39 36.46466856972182 39.0 35.0 41.0 30.0 41.0 40-41 36.29655898928275 39.0 35.0 40.0 29.0 41.0 42-43 36.07958531721406 39.0 35.0 40.0 28.0 41.0 44-45 35.82525046391261 38.0 35.0 40.0 27.5 41.0 46-47 35.558873753246246 38.0 34.0 40.0 26.5 41.0 48-49 35.31489541016512 38.0 34.0 40.0 26.0 41.0 50-51 34.6182959725509 37.5 33.5 39.5 24.5 40.5 52-53 34.715755748672294 38.0 33.5 39.5 25.5 40.5 54-55 35.180793586276195 38.0 34.0 40.0 26.0 41.0 56-57 35.11451429283614 38.0 34.0 40.0 26.0 41.0 58-59 34.883439102737825 37.5 34.0 40.0 26.0 41.0 60-61 34.66924516110306 37.0 33.5 40.0 26.0 41.0 62-63 34.37710063696759 36.5 33.0 40.0 25.0 41.0 64-65 34.13731636348277 36.0 33.0 40.0 25.0 41.0 66-67 33.982882282767335 35.5 33.0 39.0 25.0 41.0 68-69 33.708431094738046 35.0 33.0 39.0 24.0 41.0 70-71 33.454760705153404 35.0 32.5 39.0 24.0 41.0 72-73 33.15904485759493 35.0 32.0 38.0 23.5 40.0 74-75 32.84261182824481 35.0 32.0 37.0 23.0 40.0 76-77 32.533158944985175 35.0 32.0 37.0 23.0 39.0 78-79 32.21140333967142 35.0 31.0 36.0 22.0 39.0 80-81 31.927563207540203 35.0 31.0 36.0 20.5 38.5 82-83 31.636912556387607 35.0 31.0 35.5 20.0 37.0 84-85 31.3898358274217 35.0 31.0 35.0 20.0 37.0 86-87 31.16839088776493 34.0 31.0 35.0 20.0 36.5 88-89 30.962818381061773 34.0 30.5 35.0 19.5 36.0 90-91 30.726935582182882 34.0 30.0 35.0 19.0 36.0 92-93 30.52328931282883 34.0 30.0 35.0 18.0 36.0 94-95 30.36569458201466 34.0 29.0 35.0 18.0 35.0 96-97 30.18672871310325 34.0 29.0 35.0 18.0 35.0 98-99 29.951717717253473 34.0 29.0 35.0 17.0 35.0 100 28.800359915245764 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.45269897853520646 1101 2 -0.29270216561499396 1101 3 -0.3445371584772907 1101 4 -0.32752179692223393 1101 5 -0.23159490652817283 1101 6 -0.31199155810683976 1101 7 -0.31337746428516056 1101 8 -0.2919721365873684 1101 9 -0.5205883404430409 1101 10-11 -0.48003414797358346 1101 12-13 -0.47222293402695215 1101 14-15 -0.7076840747461901 1101 16-17 -0.6425985013000926 1101 18-19 -0.6593099989966618 1101 20-21 -0.7170461069423908 1101 22-23 -0.738671374198816 1101 24-25 -0.8324295153708832 1101 26-27 -0.8371899799456983 1101 28-29 -0.8718345235452318 1101 30-31 -0.9211222975376074 1101 32-33 -0.9633626769029107 1101 34-35 -0.9824791006942348 1101 36-37 -0.9393334418035195 1101 38-39 -1.016684238554845 1101 40-41 -0.9235725587947385 1101 42-43 -1.0416855496817021 1101 44-45 -1.0089328636283028 1101 46-47 -1.1742418472035254 1101 48-49 -1.1954508684115055 1101 50-51 -1.227911841878452 1101 52-53 -1.067795792713632 1101 54-55 -0.908376678136797 1101 56-57 -0.7819316320565903 1101 58-59 -0.8095469207153627 1101 60-61 -0.7544684540927662 1101 62-63 -0.8730594054326417 1101 64-65 -0.8133870081525387 1101 66-67 -0.8367682107894012 1101 68-69 -0.846325388956636 1101 70-71 -0.9279353828261705 1101 72-73 -0.8743618272614313 1101 74-75 -0.8575201486032853 1101 76-77 -0.9352082388790635 1101 78-79 -0.9840633807907864 1101 80-81 -1.1033840600310683 1101 82-83 -1.0311051393018964 1101 84-85 -1.111979666647791 1101 86-87 -1.1257417630317867 1101 88-89 -1.1830447304576204 1101 90-91 -1.2318455132657036 1101 92-93 -1.3925717028309421 1101 94-95 -1.3534050952030157 1101 96-97 -1.4900039394983722 1101 98-99 -1.5022419768028037 1101 100 -1.5445237954436415 1102 1 -0.4779740185706203 1102 2 -0.4207148947179462 1102 3 -0.44951717331968766 1102 4 -0.3077847663747164 1102 5 -0.30241417807209103 1102 6 -0.2950238749255476 1102 7 -0.36928793845384433 1102 8 -0.4120775546105335 1102 9 -0.5470509093015536 1102 10-11 -0.5703981019169291 1102 12-13 -0.5436792089141775 1102 14-15 -0.721856713890233 1102 16-17 -0.7368139408193812 1102 18-19 -0.7266869787538539 1102 20-21 -0.7021057540551539 1102 22-23 -0.7910492413477144 1102 24-25 -0.7974773146051888 1102 26-27 -0.7933280580950992 1102 28-29 -0.9315803457804606 1102 30-31 -0.8725179351336863 1102 32-33 -0.9435853197130157 1102 34-35 -0.952546933053263 1102 36-37 -0.9332082119896299 1102 38-39 -1.0480535353500215 1102 40-41 -1.1737813185500556 1102 42-43 -1.123255349035233 1102 44-45 -1.230053711303242 1102 46-47 -1.1833466574147664 1102 48-49 -1.1993575504367868 1102 50-51 -1.1866918119058454 1102 52-53 -1.1131435988689091 1102 54-55 -0.8389334804567596 1102 56-57 -0.8482810533332952 1102 58-59 -0.8636167937327031 1102 60-61 -0.8541377550679314 1102 62-63 -0.9344140105844474 1102 64-65 -0.9156532147003276 1102 66-67 -0.9271178773836652 1102 68-69 -0.9733919323706459 1102 70-71 -0.9956948660969474 1102 72-73 -0.9990703070943425 1102 74-75 -1.0542551981087769 1102 76-77 -1.1269843448123922 1102 78-79 -1.062752274963671 1102 80-81 -1.1317504438516224 1102 82-83 -1.1753770429729542 1102 84-85 -1.1957035322768128 1102 86-87 -1.1644686677204348 1102 88-89 -1.2926753973444747 1102 90-91 -1.3551722659671093 1102 92-93 -1.4077235564517387 1102 94-95 -1.3918528312577791 1102 96-97 -1.4597437452863566 1102 98-99 -1.4192779446045343 1102 100 -1.4998532507495916 1103 1 -0.47280662992793054 1103 2 -0.44064340137583 1103 3 -0.523450973739358 1103 4 -0.36976482225185237 1103 5 -0.4198258513811126 1103 6 -0.3828139721528174 1103 7 -0.3721480658243195 1103 8 -0.4173183000414795 1103 9 -0.5772228581381498 1103 10-11 -0.5397188889210938 1103 12-13 -0.6057857035488965 1103 14-15 -0.7906908396256043 1103 16-17 -0.817670312427289 1103 18-19 -0.7502087213646931 1103 20-21 -0.7904722729146698 1103 22-23 -0.834866405786336 1103 24-25 -0.8073031217595101 1103 26-27 -0.8126320478382709 1103 28-29 -0.892423371439584 1103 30-31 -0.8834288059798112 1103 32-33 -0.9016302891301962 1103 34-35 -0.9416892754050608 1103 36-37 -0.9247822203278915 1103 38-39 -0.9754733579551953 1103 40-41 -0.8933836565188216 1103 42-43 -1.0207133854091808 1103 44-45 -1.0548816377994683 1103 46-47 -1.0957208583966107 1103 48-49 -1.149782206191361 1103 50-51 -1.233017279834236 1103 52-53 -1.1082710152795698 1103 54-55 -0.8952732654255797 1103 56-57 -0.772878608019532 1103 58-59 -0.8290639092624161 1103 60-61 -0.8180858462823082 1103 62-63 -0.7993553710838555 1103 64-65 -0.7911229802866728 1103 66-67 -0.8399192274635112 1103 68-69 -0.8263328965596699 1103 70-71 -0.8719163747271992 1103 72-73 -0.8266674851544948 1103 74-75 -0.8406920003827096 1103 76-77 -0.9091755081408195 1103 78-79 -0.9659478226396807 1103 80-81 -1.0240850531643915 1103 82-83 -0.9212523311837693 1103 84-85 -1.0024917909913746 1103 86-87 -1.0611275875253234 1103 88-89 -1.0753225144489456 1103 90-91 -1.199563489923154 1103 92-93 -1.2242479275714757 1103 94-95 -1.281581109315436 1103 96-97 -1.3196725668928835 1103 98-99 -1.3122291315016206 1103 100 -1.4038410254930724 1104 1 -0.5852479640520869 1104 2 -0.5160875340924314 1104 3 -0.5800324980296665 1104 4 -0.43698043087000826 1104 5 -0.4397446291903506 1104 6 -0.4843154113939576 1104 7 -0.5417196900890318 1104 8 -0.47280678965586986 1104 9 -0.606405506529974 1104 10-11 -0.6639786032994763 1104 12-13 -0.5838556540571389 1104 14-15 -0.8469864485066196 1104 16-17 -0.9286918109105997 1104 18-19 -0.9250891411007345 1104 20-21 -0.7965098054166191 1104 22-23 -0.8203042671509309 1104 24-25 -0.8763279452061994 1104 26-27 -0.9397873950388487 1104 28-29 -1.01193208245963 1104 30-31 -0.9826650140629383 1104 32-33 -1.0317067759154455 1104 34-35 -1.1401374862400075 1104 36-37 -1.10916717233021 1104 38-39 -1.0983639280812696 1104 40-41 -1.0703734191519487 1104 42-43 -1.1336078688485998 1104 44-45 -1.1583502546886706 1104 46-47 -1.1625979982683816 1104 48-49 -1.1084929491964601 1104 50-51 -1.2014625862871355 1104 52-53 -0.9472973927123931 1104 54-55 -0.8422261576115346 1104 56-57 -0.9588497920139432 1104 58-59 -0.9201407366103069 1104 60-61 -0.9268093909891135 1104 62-63 -0.9443922344440168 1104 64-65 -0.853028221407726 1104 66-67 -0.9262035022964028 1104 68-69 -0.9306146115676341 1104 70-71 -0.896828505573346 1104 72-73 -0.9006262918941559 1104 74-75 -0.899948210561007 1104 76-77 -0.8905509778640557 1104 78-79 -0.9702977597006637 1104 80-81 -1.0043926792804818 1104 82-83 -0.9664104531386961 1104 84-85 -0.9858104907405085 1104 86-87 -1.0120049196481098 1104 88-89 -1.0817009252777794 1104 90-91 -1.082776058604253 1104 92-93 -1.0977242512526146 1104 94-95 -1.076022993133197 1104 96-97 -1.1750374463165727 1104 98-99 -1.2097180844770428 1104 100 -1.1398775516408222 1105 1 -0.5506079711819218 1105 2 -0.5493729770956577 1105 3 -0.5358497399664586 1105 4 -0.434159645233116 1105 5 -0.39007055455390116 1105 6 -0.39660486726432254 1105 7 -0.39783934259849474 1105 8 -0.5097188664570069 1105 9 -0.7150230657531438 1105 10-11 -0.6454763926833706 1105 12-13 -0.6676497910900636 1105 14-15 -0.850409616360956 1105 16-17 -0.8447717830903301 1105 18-19 -0.8595858599563613 1105 20-21 -0.8470420706762525 1105 22-23 -0.8082593052422808 1105 24-25 -0.842024912484959 1105 26-27 -0.8896834767978063 1105 28-29 -0.9040637230797657 1105 30-31 -0.957321724813653 1105 32-33 -0.9579753789395298 1105 34-35 -1.0032473940339415 1105 36-37 -1.0486070450777802 1105 38-39 -0.9449562822337967 1105 40-41 -0.9830917230167202 1105 42-43 -1.0245091508761845 1105 44-45 -1.0748065992298734 1105 46-47 -1.1831259966090428 1105 48-49 -1.149138316915824 1105 50-51 -1.1612837602373673 1105 52-53 -0.974676317983274 1105 54-55 -0.9049809014436292 1105 56-57 -0.864160261385301 1105 58-59 -0.7881465163240833 1105 60-61 -0.8417024753272315 1105 62-63 -0.8836261300273947 1105 64-65 -0.8630566414495533 1105 66-67 -0.8231859016456937 1105 68-69 -0.9150808920195672 1105 70-71 -0.8105456347575952 1105 72-73 -0.784064416477058 1105 74-75 -0.8721736602372303 1105 76-77 -0.8108136606728316 1105 78-79 -0.9088256725329984 1105 80-81 -0.8870602079130556 1105 82-83 -0.9987862889106509 1105 84-85 -0.9654450051545922 1105 86-87 -1.0728679026441412 1105 88-89 -1.0561565861835476 1105 90-91 -1.0936101809533803 1105 92-93 -1.1404857218584112 1105 94-95 -1.1371800172347868 1105 96-97 -1.1877241709478774 1105 98-99 -1.1773003569792593 1105 100 -1.2376591304652678 1106 1 -0.6421190792437663 1106 2 -0.5584823621948622 1106 3 -0.6246357168547512 1106 4 -0.487920299215979 1106 5 -0.4879886868416108 1106 6 -0.45501541250288113 1106 7 -0.5035843687141437 1106 8 -0.5153656776699407 1106 9 -0.6919128109177706 1106 10-11 -0.615133144546185 1106 12-13 -0.7279361762314878 1106 14-15 -0.8675166539364696 1106 16-17 -0.881954300490392 1106 18-19 -0.9317996408793832 1106 20-21 -0.7873404767006633 1106 22-23 -0.8240183063196298 1106 24-25 -0.8895098869393081 1106 26-27 -0.9337656494039166 1106 28-29 -0.965711588622078 1106 30-31 -1.0440615434261389 1106 32-33 -1.001630087527552 1106 34-35 -1.0400738712497315 1106 36-37 -1.0583223979700662 1106 38-39 -1.136280002879026 1106 40-41 -1.1049911175301261 1106 42-43 -1.1150430087499288 1106 44-45 -1.153511962062197 1106 46-47 -1.110430870638929 1106 48-49 -1.1599863868253095 1106 50-51 -1.1201643807126658 1106 52-53 -1.1604122243208437 1106 54-55 -1.0099587073461223 1106 56-57 -0.9164626921967027 1106 58-59 -0.976240010680641 1106 60-61 -1.0574815852089117 1106 62-63 -1.0398082764551049 1106 64-65 -0.9560801699660288 1106 66-67 -1.0304817290700328 1106 68-69 -0.9891745311337203 1106 70-71 -0.996666639456663 1106 72-73 -0.9509380994024568 1106 74-75 -0.9784139837898032 1106 76-77 -0.9785001292160587 1106 78-79 -0.9598459407294335 1106 80-81 -0.9069249136352049 1106 82-83 -0.9871372836131052 1106 84-85 -0.9968370637370683 1106 86-87 -0.9902465814676695 1106 88-89 -0.967624834240123 1106 90-91 -1.1182023470176254 1106 92-93 -1.2230121915882748 1106 94-95 -1.3276119817357426 1106 96-97 -1.3119096304294686 1106 98-99 -1.1264996373881644 1106 100 -1.3087305951268782 1107 1 -0.6091847742453425 1107 2 -0.5836705647123175 1107 3 -0.5614710491944166 1107 4 -0.45666955420298194 1107 5 -0.3790238447296588 1107 6 -0.4403070678704495 1107 7 -0.4403606897504915 1107 8 -0.5084028959722104 1107 9 -0.7304162747294498 1107 10-11 -0.6564716353394502 1107 12-13 -0.6986980328608468 1107 14-15 -0.8060824177741139 1107 16-17 -0.814302345819705 1107 18-19 -0.8433592951963576 1107 20-21 -0.8393246542933923 1107 22-23 -0.9163318161714216 1107 24-25 -0.8963428073817283 1107 26-27 -0.9294449253418762 1107 28-29 -0.9888197211199881 1107 30-31 -0.92566290441777 1107 32-33 -1.0363736291463184 1107 34-35 -1.12874495451657 1107 36-37 -1.1976597418134602 1107 38-39 -1.0831605258564423 1107 40-41 -1.0801687712909 1107 42-43 -1.0783359328291482 1107 44-45 -1.09203840176108 1107 46-47 -1.008631767450133 1107 48-49 -0.9251468147562534 1107 50-51 -1.0319603751112254 1107 52-53 -0.8903019362980018 1107 54-55 -0.8100106945100336 1107 56-57 -0.7983690473941039 1107 58-59 -0.859081907717254 1107 60-61 -0.9129219041404681 1107 62-63 -0.8555572577541284 1107 64-65 -0.855564026215049 1107 66-67 -0.8194430794393739 1107 68-69 -0.7765190244920035 1107 70-71 -0.8471155908773724 1107 72-73 -0.7686436636401055 1107 74-75 -0.7546502925526006 1107 76-77 -0.7890756775117467 1107 78-79 -0.7293014768228474 1107 80-81 -0.8009373505026467 1107 82-83 -0.856417233788143 1107 84-85 -0.8846876063123119 1107 86-87 -0.912567285102238 1107 88-89 -0.8881989388104117 1107 90-91 -0.8953165979198872 1107 92-93 -0.9248495385685658 1107 94-95 -0.9839870168752611 1107 96-97 -0.9978787651714356 1107 98-99 -0.9619783383856308 1107 100 -0.9840605678147938 1108 1 -0.6452378933646585 1108 2 -0.5847205223619127 1108 3 -0.6198854444086876 1108 4 -0.43966999260176465 1108 5 -0.3986725193612486 1108 6 -0.4628494790791038 1108 7 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1.2556838726031927 2311 70-71 1.1854900742421037 2311 72-73 1.1570140085496519 2311 74-75 1.2668019962392592 2311 76-77 1.2177044501660959 2311 78-79 1.3215137354900506 2311 80-81 1.2654091512222259 2311 82-83 1.2992103275008624 2311 84-85 1.3113859547663225 2311 86-87 1.280250870104549 2311 88-89 1.349306598345514 2311 90-91 1.4039842254025956 2311 92-93 1.4248428032293354 2311 94-95 1.4581354114882856 2311 96-97 1.4901058514925545 2311 98-99 1.5546480566099952 2311 100 1.6847398749791083 2312 1 0.617390839558098 2312 2 0.6238826431997957 2312 3 0.6363756273563652 2312 4 0.36548628185078513 2312 5 0.4668560761374678 2312 6 0.5321982569397363 2312 7 0.4369014832878051 2312 8 0.5599988413671539 2312 9 0.7525060112413726 2312 10-11 0.712088878774189 2312 12-13 0.8553936023895332 2312 14-15 1.073793496353126 2312 16-17 1.11049813381306 2312 18-19 1.0047693738904826 2312 20-21 1.0146659973722976 2312 22-23 1.0950806038845684 2312 24-25 1.08322313022515 2312 26-27 1.1112841007696872 2312 28-29 1.1486268873165884 2312 30-31 1.1387618375634219 2312 32-33 1.2083428796448885 2312 34-35 1.2329505472540205 2312 36-37 1.1999739967374197 2312 38-39 1.2016261792092564 2312 40-41 1.248432933276618 2312 42-43 1.20133762814271 2312 44-45 1.2499775683682088 2312 46-47 1.354179993485502 2312 48-49 1.4162794796482814 2312 50-51 1.3512706195802124 2312 52-53 1.2445301200684327 2312 54-55 1.1551965077996513 2312 56-57 1.1025706047023505 2312 58-59 1.0870132085518946 2312 60-61 1.0829791042910486 2312 62-63 1.0537152929281461 2312 64-65 1.1000869277626748 2312 66-67 1.0793937599915395 2312 68-69 1.062039394005133 2312 70-71 1.0085047377810739 2312 72-73 1.0842105313223627 2312 74-75 1.0921291604522807 2312 76-77 1.0748833857174986 2312 78-79 1.0663545372669532 2312 80-81 1.0909406183429944 2312 82-83 1.1251283089349506 2312 84-85 1.1416997972319933 2312 86-87 1.1449460851547606 2312 88-89 1.0349198840407254 2312 90-91 1.1038374101001232 2312 92-93 1.2251371644193831 2312 94-95 1.304317550133561 2312 96-97 1.2753518119160177 2312 98-99 0.941659540563041 2312 100 1.3516925383817977 2313 1 0.6567785153355423 2313 2 0.655312192015483 2313 3 0.6698042841211134 2313 4 0.5690741614901782 2313 5 0.43916857862019043 2313 6 0.5781153496885807 2313 7 0.627753453904397 2313 8 0.6236377660713615 2313 9 0.7714720246613993 2313 10-11 0.8185185678289386 2313 12-13 0.9267456025867844 2313 14-15 1.0954620308085268 2313 16-17 1.0845237951495292 2313 18-19 1.0774964834008287 2313 20-21 1.0619629936536157 2313 22-23 1.09963165524384 2313 24-25 1.1206185306315035 2313 26-27 1.1269178137334421 2313 28-29 1.175859154135047 2313 30-31 1.1877287794965596 2313 32-33 1.2143607746068668 2313 34-35 1.2477797771595434 2313 36-37 1.2481964893492616 2313 38-39 1.2479700390176731 2313 40-41 1.2485459724901347 2313 42-43 1.3130345091540363 2313 44-45 1.326654315614931 2313 46-47 1.3935050671941767 2313 48-49 1.3282607915854356 2313 50-51 1.2831758267090834 2313 52-53 1.1857618695025707 2313 54-55 1.0631105524033728 2313 56-57 1.0231419592219524 2313 58-59 1.085476000810928 2313 60-61 1.045477701084998 2313 62-63 1.0146897030157476 2313 64-65 1.012945133408337 2313 66-67 1.020995243212127 2313 68-69 1.0374882536103627 2313 70-71 1.0541649402745534 2313 72-73 1.0332031027609574 2313 74-75 1.0380237937509165 2313 76-77 0.8839721396333502 2313 78-79 1.0040593148468062 2313 80-81 1.0573886653443445 2313 82-83 1.1282257578688508 2313 84-85 1.1129242972846072 2313 86-87 1.137043964133401 2313 88-89 1.2075765609239681 2313 90-91 1.2199480304832022 2313 92-93 1.2968144947997402 2313 94-95 1.311519185151532 2313 96-97 1.3079691856582905 2313 98-99 1.3901482216067578 2313 100 1.5393068454543553 2314 1 0.5782498749548957 2314 2 0.5664035616529688 2314 3 0.5681198841434849 2314 4 0.425623474103908 2314 5 0.46136044533462695 2314 6 0.4903267361472601 2314 7 0.5123312496724921 2314 8 0.5723979649928523 2314 9 0.7062689419679344 2314 10-11 0.683489644113429 2314 12-13 0.7645142006423171 2314 14-15 0.9147636109830941 2314 16-17 0.856162062818214 2314 18-19 0.8268815308282385 2314 20-21 0.8619273468765698 2314 22-23 0.8950206983354292 2314 24-25 0.9213838339900491 2314 26-27 0.9810199930560728 2314 28-29 0.8974022513971462 2314 30-31 0.9788929960536024 2314 32-33 0.9296399696889139 2314 34-35 0.9751344045760746 2314 36-37 1.0143302622106987 2314 38-39 0.999801873284035 2314 40-41 1.0508580902274218 2314 42-43 1.105377056728841 2314 44-45 1.1203791285433695 2314 46-47 1.1205345492645833 2314 48-49 1.0814069078441975 2314 50-51 1.1266147284230001 2314 52-53 1.0368192225778827 2314 54-55 0.9468875870097904 2314 56-57 0.9004310000962548 2314 58-59 0.9004584564738707 2314 60-61 0.9223237083939964 2314 62-63 0.9489541076144192 2314 64-65 0.9971825840294457 2314 66-67 0.9304873267174258 2314 68-69 0.9274747659130682 2314 70-71 0.9304845292840298 2314 72-73 0.9034671648129304 2314 74-75 0.9955111066730566 2314 76-77 1.014878818147423 2314 78-79 1.0187346186286632 2314 80-81 1.0045316886042563 2314 82-83 0.9582286309020738 2314 84-85 1.0555582772614258 2314 86-87 1.1633933984211318 2314 88-89 1.1684403177956852 2314 90-91 1.211980512829257 2314 92-93 1.2561660352645916 2314 94-95 1.2113659147187903 2314 96-97 1.2747521563129105 2314 98-99 1.324307603959415 2314 100 1.418537971118976 2315 1 0.5005580935355596 2315 2 0.5105827938731196 2315 3 0.5446991643362473 2315 4 0.2879318562275728 2315 5 0.3798251309504721 2315 6 0.4768555633259268 2315 7 0.4737957499396117 2315 8 0.479764895508886 2315 9 0.6433080232924127 2315 10-11 0.5332213654579689 2315 12-13 0.5983694405598925 2315 14-15 0.8485567727053152 2315 16-17 0.8410645911929251 2315 18-19 0.6431291185690569 2315 20-21 0.7711011549207143 2315 22-23 0.6667851364562125 2315 24-25 0.7609979679208578 2315 26-27 0.8864162986197996 2315 28-29 0.8940885295946828 2315 30-31 0.9232348172683942 2315 32-33 0.9770475406393118 2315 34-35 1.0508948795049307 2315 36-37 1.0590857109181613 2315 38-39 1.1178427781129159 2315 40-41 1.1346277426440423 2315 42-43 1.243996031532319 2315 44-45 1.2432679247579017 2315 46-47 1.2731532133363714 2315 48-49 1.3214373949842582 2315 50-51 1.1705990719068993 2315 52-53 1.2531717504074464 2315 54-55 1.0860185876748645 2315 56-57 1.0472334619634793 2315 58-59 1.0115442184206884 2315 60-61 1.0156246465856569 2315 62-63 1.0357375848655863 2315 64-65 1.086467315110255 2315 66-67 1.0652391229646625 2315 68-69 1.057518159321198 2315 70-71 0.9892547268439742 2315 72-73 0.7088256510589659 2315 74-75 0.9991745379141292 2315 76-77 1.0170298855380366 2315 78-79 1.0523959919537091 2315 80-81 0.7789095437923912 2315 82-83 0.8227475010380338 2315 84-85 1.0434385147258638 2315 86-87 1.193935802394133 2315 88-89 1.2226878019592746 2315 90-91 1.2478664797499626 2315 92-93 1.321526061540503 2315 94-95 1.3151126847446335 2315 96-97 1.1844289272109805 2315 98-99 0.573158850702189 2315 100 1.1000382600449328 2316 1 0.35200190738600057 2316 2 0.32061405475870686 2316 3 0.40826695991178497 2316 4 0.22994834575668222 2316 5 0.1797447106142087 2316 6 0.2218781829455807 2316 7 0.22635984819579136 2316 8 0.2763181336594869 2316 9 0.3567288109412843 2316 10-11 0.30426578817323957 2316 12-13 0.13160448859368756 2316 14-15 0.1493116934106382 2316 16-17 0.3110647256001684 2316 18-19 0.3370008201312018 2316 20-21 0.4108741634813313 2316 22-23 0.3722025325622127 2316 24-25 0.45191551019473764 2316 26-27 0.512545571510195 2316 28-29 0.46883531194118433 2316 30-31 0.35028813998355446 2316 32-33 0.5620767014116623 2316 34-35 0.5378795537185681 2316 36-37 0.5821965890567142 2316 38-39 0.701987651204476 2316 40-41 0.7619664007444911 2316 42-43 0.6128464239454772 2316 44-45 0.5366758653140522 2316 46-47 0.38412734106133684 2316 48-49 0.3514084190218796 2316 50-51 0.4039761435986122 2316 52-53 0.5366699566307247 2316 54-55 0.19293137641242453 2316 56-57 0.13295515607722308 2316 58-59 0.13190389221429655 2316 60-61 0.20796147338734983 2316 62-63 0.3912034235836188 2316 64-65 0.31978944783965346 2316 66-67 0.2891936305917113 2316 68-69 0.24691404354727098 2316 70-71 0.02989275804660707 2316 72-73 -0.06010275356060646 2316 74-75 0.16393687789950206 2316 76-77 0.3726557248832947 2316 78-79 0.4030541954440352 2316 80-81 0.5542220686437211 2316 82-83 0.5506745828662822 2316 84-85 0.5783843990079411 2316 86-87 0.6406689782469748 2316 88-89 0.6133416820164896 2316 90-91 0.5479504282764651 2316 92-93 0.55057656394359 2316 94-95 0.657349154135062 2316 96-97 0.5626150790082853 2316 98-99 0.4604230359870556 2316 100 0.35046707516664455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 35.0 1 623.0 2 251.0 3 221.0 4 148.0 5 143.0 6 145.0 7 589.0 8 1868.0 9 3818.0 10 6010.0 11 9264.0 12 13560.0 13 18290.0 14 22325.0 15 26900.0 16 32674.0 17 41502.0 18 51255.0 19 63834.0 20 79412.0 21 99752.0 22 108700.0 23 107175.0 24 103022.0 25 104143.0 26 113785.0 27 129243.0 28 152173.0 29 181319.0 30 219774.0 31 272330.0 32 344474.0 33 451266.0 34 645154.0 35 889612.0 36 1338673.0 37 1828644.0 38 1628429.0 39 521224.0 40 504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62700431779289 21.528444553626823 13.290693220190782 22.55385790838951 2 15.146750092790798 19.95263111785124 42.15710713969851 22.74351164965945 3 18.896943517605465 27.161485785052648 28.770268066908876 25.17130263043301 4 11.996381942132924 16.702656138519217 40.49959623048564 30.801365688862216 5 12.568800294347055 38.791628756441966 35.02939100293598 13.610179946275004 6 28.872629709068885 39.14793080239996 18.153315747827087 13.826123740704062 7 26.63182750398631 32.51088676123616 23.35723212592314 17.500053608854394 8 25.805525922976063 35.77376837903713 19.899003703468164 18.521701994518647 9 25.976513409096373 15.673682239640744 22.237263629921284 36.1125407213416 10-11 25.39464559107893 26.766325650801676 27.281307360294498 20.557721397824892 12-13 25.48689949946385 24.48030592726266 28.11896842905668 21.913826144216817 14-15 22.90650950671152 25.587739600585657 26.187900613302993 25.317850279399828 16-17 21.018048566393016 28.434613382801377 27.43658663834213 23.110751412463475 18-19 20.41427052629635 28.299650148548345 30.14002642420288 21.146052900952423 20-21 22.042069546653746 27.369674164339447 29.6260466242991 20.962209664707704 22-23 22.37972051982503 27.203137319052605 29.210523892259864 21.206618268862503 24-25 21.692140115719898 27.17648598299088 30.1829712609754 20.948402640313823 26-27 21.627483268934824 27.43547666854502 29.15493689792397 21.78210316459618 28-29 20.809122066924314 27.717864718870967 29.77532745983319 21.69768575437153 30-31 21.783307948258148 27.42086526237499 29.64805443636705 21.147772352999812 32-33 22.05632008174833 27.70649686406817 28.747024253165915 21.490158801017586 34-35 22.03885422755178 27.521186068095314 29.529469964061285 20.91048974029162 36-37 21.630192671166817 27.138089700446134 29.216633197101615 22.01508443128543 38-39 21.80434913018489 27.397879195637913 29.673784799641258 21.12398687453594 40-41 21.608362107701012 26.41094125949835 30.218746936729136 21.7619496960715 42-43 21.571128849208133 26.626521189132983 29.52195335366017 22.28039660799871 44-45 21.706725430251478 27.57094499729294 29.30748448879907 21.414845083656513 46-47 21.70879123767151 27.441099300356186 28.596628663887397 22.253480798084908 48-49 22.381214850333205 27.33285756793265 28.578234388596535 21.707693193137615 50-51 22.098540006411344 27.134661185509778 28.517960064325738 22.24883874375314 52-53 21.537437997090052 27.77268791330937 28.27097483249067 22.41889925710991 54-55 21.519713078124227 27.7214055358721 28.25454471279713 22.50433667320654 56-57 22.38674246587204 27.326476311083137 27.834373241148715 22.452407981896112 58-59 21.4963421221624 27.663849925531043 28.65623858376795 22.18356936853861 60-61 21.56612402973324 28.317335738054144 27.968937727900954 22.14760250431166 62-63 21.93561374616248 27.5262645475072 27.68168007357549 22.85644163275483 64-65 21.221537913539017 27.67180037793791 28.067147638507212 23.039514070015862 66-67 21.719077284511748 27.317743137716576 27.915798894541783 23.047380683229896 68-69 21.309575877797688 27.38153773862029 28.20905048320535 23.09983590037667 70-71 21.388643600854824 26.941103170133257 28.342029877548292 23.32822335146363 72-73 21.987336431772608 26.98810376463546 28.492128216039305 22.53243158755263 74-75 22.148506831479082 27.319147080762303 28.035730192373652 22.496615895384963 76-77 21.79229468536198 27.30684425483208 27.756593220779894 23.14426783902604 78-79 21.648357954533335 27.32583024979773 27.790018964517866 23.23579283115107 80-81 21.250535258373056 27.725931063404246 28.29088814647895 22.73264553174375 82-83 22.195118157011898 26.71652956788116 29.29708272424193 21.791269550865014 84-85 22.249543246406695 27.167473265497772 28.053194684381307 22.52978880371423 86-87 21.11298151195731 27.747936742427175 28.512646691881617 22.626435053733893 88-89 22.13092374016159 26.6344214544652 28.311772878997267 22.922881926375947 90-91 21.795602389150147 26.743930443454556 28.429119886270783 23.03134728112451 92-93 22.088682774609623 26.910316789452327 28.33253410490743 22.668466331030615 94-95 20.98131977016312 26.853605629001454 28.965709068500487 23.199365532334937 96-97 21.390722905125422 26.772644613517855 28.478476126969788 23.35815635438693 98-99 20.8228513676562 27.196379064370323 28.33757069326948 23.643198874703998 100 21.67823318636527 26.653723708923316 27.468368288199486 24.19967481651193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 894.0 1 647.0 2 619.0 3 894.0 4 789.0 5 714.5 6 1101.5 7 2007.0 8 3332.5 9 4217.0 10 4281.5 11 4506.5 12 5351.0 13 6519.0 14 7757.5 15 9263.0 16 10831.0 17 12046.0 18 13409.5 19 14562.5 20 15634.5 21 17231.5 22 19234.5 23 22101.0 24 25369.5 25 29420.0 26 36241.5 27 44718.5 28 54614.0 29 72963.5 30 100369.5 31 126907.0 32 164010.0 33 205511.5 34 237516.0 35 268551.0 36 298367.0 37 342372.0 38 358863.5 39 340858.0 40 316674.0 41 288938.0 42 278963.5 43 285800.0 44 304680.5 45 348923.0 46 386879.5 47 408403.5 48 424171.0 49 414126.5 50 392177.0 51 397649.0 52 395299.0 53 366800.0 54 339394.5 55 286504.0 56 243436.5 57 195138.5 58 147585.0 59 139845.0 60 115050.0 61 76305.0 62 54525.5 63 46378.5 64 34074.5 65 14826.5 66 7346.5 67 4667.5 68 3394.5 69 2673.5 70 2009.0 71 1412.5 72 992.5 73 716.5 74 579.5 75 431.0 76 307.5 77 228.0 78 162.5 79 110.0 80 93.0 81 85.0 82 66.0 83 51.0 84 45.5 85 37.5 86 23.0 87 21.0 88 21.0 89 20.0 90 18.0 91 11.5 92 9.5 93 7.5 94 5.5 95 4.0 96 3.0 97 2.5 98 2.5 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057249786028534595 2 0.1322477339623354 3 0.01706153899451149 4 0.016458143103242184 5 0.01913181110421136 6 0.05315085531887756 7 0.05868545211465812 8 0.049748950897410944 9 0.04132221517451198 10-11 0.04019344872274094 12-13 0.04125979490989791 14-15 0.019163021236518394 16-17 0.01819550713500036 18-19 0.018263129088332268 20-21 0.021649428443645372 22-23 0.026315343223546835 24-25 0.025181375083057966 26-27 0.019990089742654774 28-29 0.018824911469858867 30-31 0.016947101842719036 32-33 0.02372490224206308 34-35 0.023844541082573376 36-37 0.023808129261548502 38-39 0.021727453774412955 40-41 0.02535303081074665 42-43 0.03253656293008212 44-45 0.04208166172731645 46-47 0.0477358973636073 48-49 0.04042752471504369 50-51 0.028448035597860775 52-53 0.03998017948530955 54-55 0.036567871686407245 56-57 0.038690160683285506 58-59 0.04403749668522386 60-61 0.023688490421038207 62-63 0.026434982064057134 64-65 0.025836787861505665 66-67 0.03251575617521077 68-69 0.03922593462122291 70-71 0.041036122295030834 72-73 0.04481254830418186 74-75 0.02737128603326813 76-77 0.020941998778019288 78-79 0.020260577555982394 80-81 0.02515536663946877 82-83 0.020583082256488406 84-85 0.012770145802294422 86-87 0.014871628044301326 88-89 0.01918382799138975 90-91 0.021508982848263726 92-93 0.020312594443160782 94-95 0.02410462551846532 96-97 0.02531661898972178 98-99 0.028115127519919084 100 0.030367458734743315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9612263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.116109462056414 #Duplication Level Percentage of deduplicated Percentage of total 1 75.76453359770497 47.81962595894399 2 14.51990246608243 18.328795068552832 3 5.00257614761559 9.472294311755343 4 2.069915978187207 5.225801738260935 5 1.0346592113551596 3.265183201990861 6 0.5513783813336192 2.088051496275851 7 0.30659756876081273 1.354587199749548 8 0.18402917053001105 0.9292164217106901 9 0.12635729850553507 0.717766297542458 >10 0.39433542409018113 3.994335206828693 >50 0.021296930079304124 0.9403820429395429 >100 0.021123169542915446 2.6998912686230643 >500 0.002263742757643818 0.989635614548469 >1k 9.412688088597877E-4 0.9262746810716525 >5k 2.2411161405108245E-5 0.09775646241755302 >10k+ 6.723348421532474E-5 1.1504030287884779 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36069 0.37523942072746036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27992 0.29121134117949127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17268 0.17964552155928318 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.0 0.0 4 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.0 0.0 5 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.0 0.0 6 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.0 0.0 7 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.0 0.0 8 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.0 0.0 9 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.0403377435677737E-5 0.0 10-11 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.0403377435677737E-5 0.0 12-13 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.0403377435677737E-5 0.0 14-15 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.5605066153516605E-5 0.0 16-17 1.0403377435677737E-5 1.0403377435677737E-5 0.0 4.1613509742710955E-5 0.0 18-19 1.0403377435677737E-5 1.0403377435677737E-5 0.0 6.76219533319053E-5 0.0 20-21 1.0403377435677737E-5 1.0403377435677737E-5 0.0 8.842870820326078E-5 0.0 22-23 1.0403377435677737E-5 1.0403377435677737E-5 0.0 2.548827471741046E-4 0.0 24-25 1.0403377435677737E-5 1.0403377435677737E-5 0.0 7.594465528044748E-4 0.0 26-27 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.001544901549198144 0.0 28-29 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.004619099581440916 0.0 30-31 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.016562176877598957 0.0 32-33 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.04545755770519387 0.0 34-35 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.08812180856890828 0.0 36-37 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.1614344093581293 0.0 38-39 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.2634083149826425 0.0 40-41 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.36973083237526894 0.0 42-43 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.47501821371304553 0.0 44-45 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.599676683836054 0.0 46-47 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.7482629220611213 0.0 48-49 1.0403377435677737E-5 1.0403377435677737E-5 0.0 0.9112890481669094 0.0 50-51 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.0810253527187095 5.2016887178388685E-6 52-53 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.2439266382952692 1.0403377435677737E-5 54-55 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.4194368173238705 1.0403377435677737E-5 56-57 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.6248775132349167 1.0403377435677737E-5 58-59 1.0403377435677737E-5 1.0403377435677737E-5 0.0 1.8645245141544713 1.0403377435677737E-5 60-61 1.0403377435677737E-5 1.0403377435677737E-5 0.0 2.104036271167362 1.0403377435677737E-5 62-63 1.0403377435677737E-5 1.0403377435677737E-5 0.0 2.345087728040733 1.0403377435677737E-5 64-65 1.0403377435677737E-5 1.0403377435677737E-5 0.0 2.5924072198191 1.0403377435677737E-5 66-67 1.0403377435677737E-5 1.0403377435677737E-5 0.0 2.863779320228754 1.0403377435677737E-5 68-69 1.0403377435677737E-5 1.0403377435677737E-5 0.0 3.164592978781376 1.0403377435677737E-5 70-71 1.0403377435677737E-5 1.0403377435677737E-5 0.0 3.4932772854841776 1.0403377435677737E-5 72-73 1.0403377435677737E-5 1.0403377435677737E-5 0.0 3.825301076343833 1.0403377435677737E-5 74-75 1.0403377435677737E-5 1.0403377435677737E-5 0.0 4.137256752130066 1.0403377435677737E-5 76-77 1.0403377435677737E-5 1.0403377435677737E-5 0.0 4.487985815619069 1.0403377435677737E-5 78-79 1.0403377435677737E-5 1.0403377435677737E-5 0.0 4.86591971110237 1.0403377435677737E-5 80-81 1.0403377435677737E-5 2.0806754871355474E-5 0.0 5.273149517444539 1.0403377435677737E-5 82-83 1.0403377435677737E-5 2.0806754871355474E-5 0.0 5.6880674197116745 1.0403377435677737E-5 84-85 1.0403377435677737E-5 2.0806754871355474E-5 0.0 6.08392113282793 1.0403377435677737E-5 86-87 1.0403377435677737E-5 2.0806754871355474E-5 0.0 6.498594555725328 1.0403377435677737E-5 88 1.0403377435677737E-5 2.0806754871355474E-5 0.0 6.832563778165454 1.0403377435677737E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18845 0.0 47.946133 1 GTATCAA 34420 0.0 40.94817 1 GTACTAG 9840 0.0 37.542492 1 GCATCGT 9100 0.0 36.986088 8 GGGCACC 9515 0.0 34.477203 94 CAACGCA 39425 0.0 33.970966 5 ATCAACG 39765 0.0 33.668858 3 TATCAAC 43535 0.0 32.147106 2 TAGGCAT 12335 0.0 31.898733 5 GTGGTAT 6175 0.0 31.398489 1 CTGTGCG 12405 0.0 31.149 9 AACGCAG 44915 0.0 30.426027 6 TAGTACT 13585 0.0 29.586502 4 CTAGGCA 13815 0.0 29.093925 4 ACTGTGC 13965 0.0 28.611774 8 TACCTGG 4215 0.0 28.469645 2 TCAACGC 47685 0.0 28.382261 4 ACGCAGA 47460 0.0 28.200586 7 CGCAGAG 47550 0.0 28.085842 8 CATCGTC 12420 0.0 27.932112 9 >>END_MODULE