FastQCFastQC Report
Mon 6 Jun 2016
ERR1211234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612263
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322660.3356753763395779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269460.28032940838177234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166690.17341389847531222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199800.047.331811
GTATCAA351750.038.9442061
GGGCACC97200.035.3069194
GTACTAG97300.034.121521
CAACGCA400150.032.5630075
ATCAACG401900.032.4121673
TACCTGG47400.030.8402122
TATCAAC448550.030.3999022
ACCTGGG47950.030.0000763
TAGGCAT120450.029.8826715
GTGGTAT63600.029.8717581
CTGTGCG123750.029.6936479
AACGCAG457400.029.2475036
CTAGGCA135200.027.8165254
ACTGTGC142300.027.7377918
TAGTACT136700.027.5800744
TCAACGC483050.027.4147534
GCATCGT113200.027.1889448
ACGCAGA488050.026.7078937
CGCAGAG489100.026.6121258
Produced by FastQC (version 0.11.5)