##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211234_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9612263 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18523452801905 33.0 31.0 34.0 30.0 34.0 2 32.330081792393734 34.0 31.0 34.0 31.0 34.0 3 32.404392909349234 34.0 31.0 34.0 31.0 34.0 4 36.00804389143327 37.0 35.0 37.0 35.0 37.0 5 35.905242917302616 37.0 35.0 37.0 35.0 37.0 6 35.93229128250028 37.0 35.0 37.0 35.0 37.0 7 35.87425104785418 37.0 35.0 37.0 35.0 37.0 8 35.86568573914384 37.0 35.0 37.0 35.0 37.0 9 37.469349933517215 39.0 37.0 39.0 35.0 39.0 10-11 37.45206258921546 39.0 37.0 39.0 35.0 39.0 12-13 37.351479459103444 39.0 37.0 39.0 34.0 39.0 14-15 38.56859466912214 40.0 38.0 41.0 34.0 41.0 16-17 38.53531863412393 40.0 38.0 41.0 34.0 41.0 18-19 38.57512445300342 40.0 38.0 41.0 34.0 41.0 20-21 38.54604763727335 40.0 38.0 41.0 34.0 41.0 22-23 38.413722970334874 40.0 38.0 41.0 34.0 41.0 24-25 38.34852521201303 40.0 38.0 41.0 34.0 41.0 26-27 38.203727571748715 40.0 38.0 41.0 34.0 41.0 28-29 38.1903392052423 40.0 38.0 41.0 33.5 41.0 30-31 38.19232229704909 40.0 37.5 41.0 34.0 41.0 32-33 38.05702933846067 40.0 37.0 41.0 33.5 41.0 34-35 37.91835044463515 40.0 37.0 41.0 33.0 41.0 36-37 37.780245869260966 40.0 37.0 41.0 33.0 41.0 38-39 37.68246894617844 40.0 37.0 41.0 33.0 41.0 40-41 37.576027518181725 40.0 36.5 41.0 33.0 41.0 42-43 37.41961086582837 40.0 36.0 41.0 32.0 41.0 44-45 37.240316354223765 40.0 36.0 41.0 31.5 41.0 46-47 36.96783993529932 39.0 36.0 41.0 31.0 41.0 48-49 36.69042992269354 39.0 35.0 41.0 30.5 41.0 50-51 36.467552854098976 39.0 35.0 41.0 30.0 41.0 52-53 36.25315921963434 39.0 35.0 40.0 30.0 41.0 54-55 36.01028831608124 38.0 35.0 40.0 29.0 41.0 56-57 35.735321380615574 38.0 35.0 40.0 28.5 41.0 58-59 35.44237345565763 37.5 34.0 40.0 28.0 41.0 60-61 35.19185830641546 37.0 34.0 40.0 28.0 41.0 62-63 34.889348117087515 36.5 34.0 40.0 27.5 41.0 64-65 34.52406993025471 36.0 34.0 39.0 26.5 41.0 66-67 34.23497947361615 35.5 33.0 39.0 26.0 40.5 68-69 33.93475355387176 35.0 33.0 39.0 26.0 40.0 70-71 33.66747174936849 35.0 33.0 38.0 26.0 40.0 72-73 33.36312583207513 35.0 33.0 37.5 26.0 40.0 74-75 33.04765953657323 35.0 32.0 37.0 25.0 39.0 76-77 32.059571819872176 34.0 31.0 36.0 24.5 39.0 78-79 32.5628055536974 35.0 32.0 36.0 25.0 39.0 80-81 32.50389653300165 35.0 32.0 36.0 25.0 37.5 82-83 32.25787460247395 35.0 32.0 35.0 25.0 37.0 84-85 32.01409090658464 34.5 32.0 35.0 25.0 37.0 86-87 31.785012852852653 34.0 31.0 35.0 24.0 36.0 88-89 31.573419079357272 34.0 31.0 35.0 24.0 36.0 90-91 31.360802445792423 34.0 31.0 35.0 23.5 36.0 92-93 31.169873785184613 34.0 31.0 35.0 23.0 35.0 94-95 30.9885390672311 34.0 31.0 35.0 22.5 35.0 96-97 30.823151478481186 34.0 30.5 35.0 20.0 35.0 98-99 30.66121390977338 34.0 30.0 35.0 20.0 35.0 100 28.851190817396485 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3542742049443248 1101 2 -0.32534319260308564 1101 3 -0.3789287982270473 1101 4 -0.19623208114592927 1101 5 -0.22393783740059092 1101 6 -0.3261947426563836 1101 7 -0.3241566098619231 1101 8 -0.3485010807516957 1101 9 -0.44518016350137657 1101 10-11 -0.49146701443044805 1101 12-13 -0.5004507053526481 1101 14-15 -0.708053682878031 1101 16-17 -0.683302633903196 1101 18-19 -0.6989667970512699 1101 20-21 -0.6669491195688835 1101 22-23 -0.7107838074007233 1101 24-25 -0.6668610231325474 1101 26-27 -0.8006020786155545 1101 28-29 -0.8018675465922982 1101 30-31 -0.6597224232404457 1101 32-33 -0.7409190164723967 1101 34-35 -0.718036874031327 1101 36-37 -0.6324737483094296 1101 38-39 -0.6939705159810359 1101 40-41 -0.7430109492577657 1101 42-43 -0.7053368727984051 1101 44-45 -0.7953128302921613 1101 46-47 -0.772021108132499 1101 48-49 -0.7827671436030386 1101 50-51 -0.8119215352957596 1101 52-53 -0.7812348458779823 1101 54-55 -0.7150811209177661 1101 56-57 -0.7359052231158714 1101 58-59 -0.7600412989594787 1101 60-61 -0.8020847279974674 1101 62-63 -0.8867355763045381 1101 64-65 -0.7678890523792106 1101 66-67 -0.921222027692906 1101 68-69 -1.007296533679579 1101 70-71 -1.0056358979634794 1101 72-73 -0.9338314409239175 1101 74-75 -0.9436347420715876 1101 76-77 -0.9145877022358668 1101 78-79 -0.833457051593399 1101 80-81 -0.7126604435556168 1101 82-83 -0.7060835066120035 1101 84-85 -0.7379918897000373 1101 86-87 -0.8757501175904103 1101 88-89 -0.8159705784307683 1101 90-91 -0.8527358698364864 1101 92-93 -0.8724478605039359 1101 94-95 -0.8978399597892199 1101 96-97 -0.8997349229196203 1101 98-99 -1.0312615615157874 1101 100 -0.9601264264043223 1102 1 -0.383666399871732 1102 2 -0.34747741419609923 1102 3 -0.36814030786877794 1102 4 -0.25682654450692866 1102 5 -0.32972644082421 1102 6 -0.3726715234870781 1102 7 -0.37046509462859945 1102 8 -0.36640316341548385 1102 9 -0.5137506679715713 1102 10-11 -0.5470026940868351 1102 12-13 -0.6025764025033311 1102 14-15 -0.6808649946211389 1102 16-17 -0.7190147366240964 1102 18-19 -0.6996545427542742 1102 20-21 -0.7208282205006142 1102 22-23 -0.666646931170277 1102 24-25 -0.6997071932664412 1102 26-27 -0.8324862339111547 1102 28-29 -0.8056132304081203 1102 30-31 -0.7743750761990214 1102 32-33 -0.668829609873633 1102 34-35 -0.6979492038422777 1102 36-37 -0.7207626104128693 1102 38-39 -0.784418926316448 1102 40-41 -0.6864308541047137 1102 42-43 -0.6878064667841599 1102 44-45 -0.779194713316798 1102 46-47 -0.7666494074994006 1102 48-49 -0.7409197379732078 1102 50-51 -0.7600569848470329 1102 52-53 -0.8495298135104505 1102 54-55 -0.6658483892519982 1102 56-57 -0.7082672542629709 1102 58-59 -0.7939606991238009 1102 60-61 -0.7553608479783094 1102 62-63 -0.8130581154371441 1102 64-65 -0.7377478166228784 1102 66-67 -0.8128492642455001 1102 68-69 -0.9260781962610452 1102 70-71 -0.9605563993158057 1102 72-73 -0.9159543011621238 1102 74-75 -0.9949298885528925 1102 76-77 -0.8482106313411499 1102 78-79 -0.6929695480558244 1102 80-81 -0.6666865979699992 1102 82-83 -0.6002592019467912 1102 84-85 -0.6896833131105957 1102 86-87 -0.7272535441558325 1102 88-89 -0.8652855394437786 1102 90-91 -0.8041709432570165 1102 92-93 -0.8345896746066792 1102 94-95 -0.9272182785142782 1102 96-97 -1.1076074921001968 1102 98-99 -1.0829464408407894 1102 100 -1.0643761761187491 1103 1 -0.2991345775920031 1103 2 -0.28504671930128467 1103 3 -0.3579035583487453 1103 4 -0.2582439446321203 1103 5 -0.22272985670471002 1103 6 -0.3031470040790225 1103 7 -0.3615394500665161 1103 8 -0.3741995858613478 1103 9 -0.5374540965812429 1103 10-11 -0.5123874760308382 1103 12-13 -0.5502997831095797 1103 14-15 -0.6425452587580622 1103 16-17 -0.6959289354275526 1103 18-19 -0.6600819674323333 1103 20-21 -0.70202677068702 1103 22-23 -0.6960043440771386 1103 24-25 -0.7818737512320766 1103 26-27 -0.721260611264249 1103 28-29 -0.7852825080865315 1103 30-31 -0.7381933167121772 1103 32-33 -0.6612236001285936 1103 34-35 -0.7702476990439067 1103 36-37 -0.7268434813508549 1103 38-39 -0.7377910420758198 1103 40-41 -0.7630757718178103 1103 42-43 -0.6255227479230641 1103 44-45 -0.7440995922649734 1103 46-47 -0.777523993637196 1103 48-49 -0.8422616160382148 1103 50-51 -0.9030659123287919 1103 52-53 -0.8914885841084228 1103 54-55 -0.8799221318523394 1103 56-57 -0.8673364629151408 1103 58-59 -0.8607833528548241 1103 60-61 -0.9650210446374885 1103 62-63 -0.8925510218750858 1103 64-65 -0.8358022869908055 1103 66-67 -0.9159663372177818 1103 68-69 -0.9097036882042673 1103 70-71 -0.959865940028827 1103 72-73 -0.9248329658848533 1103 74-75 -0.9342132360370456 1103 76-77 -0.9088360143646277 1103 78-79 -0.7989544252717877 1103 80-81 -0.7769653406425192 1103 82-83 -0.827415714362985 1103 84-85 -0.8283487051101126 1103 86-87 -0.7356290784202777 1103 88-89 -0.8482035779961201 1103 90-91 -0.8496177700838174 1103 92-93 -0.9272004545113823 1103 94-95 -0.985973325894367 1103 96-97 -1.0225241816225115 1103 98-99 -1.0587831215970347 1103 100 -1.207143577359382 1104 1 -0.41066654634683175 1104 2 -0.3614774012757067 1104 3 -0.43161676183189357 1104 4 -0.2728334777494865 1104 5 -0.32342595487230597 1104 6 -0.384453671903664 1104 7 -0.38060086821158023 1104 8 -0.3720937671367963 1104 9 -0.57613996292271 1104 10-11 -0.547335651366339 1104 12-13 -0.6052298298953858 1104 14-15 -0.7552749049865426 1104 16-17 -0.7323807212037323 1104 18-19 -0.73963053059434 1104 20-21 -0.8306733840602973 1104 22-23 -0.7652934933496169 1104 24-25 -0.819465062413677 1104 26-27 -0.8719003097857865 1104 28-29 -0.9026289749650473 1104 30-31 -0.8366537171896127 1104 32-33 -0.7836273061407084 1104 34-35 -0.7556084473900171 1104 36-37 -0.8318863789706157 1104 38-39 -0.7951254056444057 1104 40-41 -0.8530269771685823 1104 42-43 -0.7334746470268385 1104 44-45 -0.8177329445699968 1104 46-47 -0.7980182166357466 1104 48-49 -0.8886967641120904 1104 50-51 -0.7806945681423798 1104 52-53 -0.7409593590539885 1104 54-55 -0.8955736779074783 1104 56-57 -0.808348547283444 1104 58-59 -0.8173040835581844 1104 60-61 -0.751126649721158 1104 62-63 -0.9037559512840403 1104 64-65 -0.954990981239142 1104 66-67 -1.0118919749867104 1104 68-69 -0.9347829305445359 1104 70-71 -1.0354682868313674 1104 72-73 -0.9313777730649448 1104 74-75 -0.9309556829363999 1104 76-77 -0.8487039861047627 1104 78-79 -0.7098190690848902 1104 80-81 -0.748226048618502 1104 82-83 -0.73843785067643 1104 84-85 -0.7788263045803667 1104 86-87 -0.8134732499478758 1104 88-89 -0.901911400512347 1104 90-91 -0.8318487117517392 1104 92-93 -0.8262666325443284 1104 94-95 -0.858128407010252 1104 96-97 -0.8621353590865688 1104 98-99 -0.9214064048192547 1104 100 -1.1032225456259823 1105 1 -0.4128889765973298 1105 2 -0.3851316917297467 1105 3 -0.4544884519400654 1105 4 -0.24312731047447045 1105 5 -0.28891999523211354 1105 6 -0.38562358364022487 1105 7 -0.42852808451502256 1105 8 -0.4838472955507953 1105 9 -0.5640698824663772 1105 10-11 -0.5481110097577897 1105 12-13 -0.5864881908807931 1105 14-15 -0.7653988401925389 1105 16-17 -0.6888795566124841 1105 18-19 -0.6756701979367392 1105 20-21 -0.8147172911548282 1105 22-23 -0.7845892179749541 1105 24-25 -0.7570892972856527 1105 26-27 -0.8209528560499777 1105 28-29 -0.7638149833721286 1105 30-31 -0.7698795554835627 1105 32-33 -0.8302542428450934 1105 34-35 -0.7636578416791693 1105 36-37 -0.7282944824563415 1105 38-39 -0.7525484780469966 1105 40-41 -0.8592068247778144 1105 42-43 -0.7926676048945538 1105 44-45 -0.7917421091808805 1105 46-47 -0.7969254107869332 1105 48-49 -0.8067495981393407 1105 50-51 -0.8542257903956951 1105 52-53 -0.9669041959837585 1105 54-55 -0.8404245018269094 1105 56-57 -0.7949452496525211 1105 58-59 -0.9127261740227723 1105 60-61 -0.9052006885460742 1105 62-63 -0.9020295378381249 1105 64-65 -0.9087379246999774 1105 66-67 -0.9257600881401231 1105 68-69 -0.9738740746501904 1105 70-71 -0.9273502385606562 1105 72-73 -0.9048562774412972 1105 74-75 -0.9416135569149517 1105 76-77 -0.9554416240092998 1105 78-79 -0.8893552045286626 1105 80-81 -0.8550995968270065 1105 82-83 -0.7723445734664267 1105 84-85 -0.8827845032077484 1105 86-87 -0.810310608550143 1105 88-89 -0.8463646842560912 1105 90-91 -0.876401329490605 1105 92-93 -0.8985774332826644 1105 94-95 -0.8680273913851231 1105 96-97 -0.9389518793771892 1105 98-99 -0.9687585135514567 1105 100 -1.0733684446270466 1106 1 -0.45210491529154595 1106 2 -0.3959686431348004 1106 3 -0.44018203106762854 1106 4 -0.27857476448453866 1106 5 -0.3073960102926563 1106 6 -0.36356230047774574 1106 7 -0.4177285503189694 1106 8 -0.41262010981652963 1106 9 -0.5784746645866008 1106 10-11 -0.5592325837143619 1106 12-13 -0.6499196865318382 1106 14-15 -0.7074342815447068 1106 16-17 -0.7529352697825047 1106 18-19 -0.728538915944533 1106 20-21 -0.7413728271629836 1106 22-23 -0.7151064840387704 1106 24-25 -0.7418756362167613 1106 26-27 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1.1685748714744122 2310 70-71 1.1605441433112418 2310 72-73 1.1896128512383157 2310 74-75 1.1358931914439268 2310 76-77 1.1301787081721706 2310 78-79 1.0060773141362773 2310 80-81 0.9681916018159384 2310 82-83 1.0280490620204503 2310 84-85 1.0121685260671036 2310 86-87 0.995783519477417 2310 88-89 1.0091090920915597 2310 90-91 1.0875933606541395 2310 92-93 1.1432663826052796 2310 94-95 1.203061463707602 2310 96-97 1.189764554630905 2310 98-99 1.1504463391185986 2310 100 1.3606643221694519 2311 1 0.37511707548184603 2311 2 0.30143035800251994 2311 3 0.4051239497523724 2311 4 0.2845915089621087 2311 5 0.3701509990300522 2311 6 0.44545235199485234 2311 7 0.46645041563569833 2311 8 0.4921571033718948 2311 9 0.6403440145124577 2311 10-11 0.6672797491063847 2311 12-13 0.7119933552877526 2311 14-15 0.8240951693772658 2311 16-17 0.6720900255698936 2311 18-19 0.812524480259043 2311 20-21 0.7458435128570997 2311 22-23 0.9272660590347073 2311 24-25 0.9309789758901914 2311 26-27 0.30241543274021865 2311 28-29 0.621090899192879 2311 30-31 0.8574355820311084 2311 32-33 0.9572796606736489 2311 34-35 1.0031045835382741 2311 36-37 1.030324677232933 2311 38-39 1.037800306505332 2311 40-41 1.0258510475167029 2311 42-43 1.012688571601963 2311 44-45 1.066840102706422 2311 46-47 0.7462317450109524 2311 48-49 0.9624064155267646 2311 50-51 1.084301842536128 2311 52-53 1.0292371658359016 2311 54-55 1.0558698580166563 2311 56-57 1.134392976809771 2311 58-59 1.1477856506179123 2311 60-61 1.1687832476605706 2311 62-63 1.1636650366626 2311 64-65 1.2187649797508655 2311 66-67 1.235094344590351 2311 68-69 1.2285264136158744 2311 70-71 1.2428892036663015 2311 72-73 1.2185946478711784 2311 74-75 1.2000084818200065 2311 76-77 1.153529331522801 2311 78-79 0.8782924808539434 2311 80-81 0.8142466527778538 2311 82-83 0.9672934238908866 2311 84-85 1.0880446624157578 2311 86-87 1.0885804136827595 2311 88-89 1.0487436008310134 2311 90-91 1.128169561157648 2311 92-93 1.1922431731995182 2311 94-95 1.2499000333085455 2311 96-97 1.2347436339125757 2311 98-99 1.2759359483428732 2311 100 1.4100602217243718 2312 1 0.25938478989701963 2312 2 0.3705890852715683 2312 3 0.4299099442270631 2312 4 0.1627825692940732 2312 5 0.31442124614027733 2312 6 0.4215156005867726 2312 7 0.3360033507265925 2312 8 0.4298007695791881 2312 9 0.619150386546572 2312 10-11 0.6187814841963188 2312 12-13 0.665346229039308 2312 14-15 0.8757042110190767 2312 16-17 0.8484433934852476 2312 18-19 0.8196214809387925 2312 20-21 0.8409802562734754 2312 22-23 0.8978477200860624 2312 24-25 0.8996764107263431 2312 26-27 0.9434385868709612 2312 28-29 0.9630458699026221 2312 30-31 0.9428561771458561 2312 32-33 0.8051606830494862 2312 34-35 0.9164535364830755 2312 36-37 0.9827840503640672 2312 38-39 0.8375846497085746 2312 40-41 0.9363331124225809 2312 42-43 1.0377706766949828 2312 44-45 0.7403941492806823 2312 46-47 0.745129567912052 2312 48-49 0.932068732130638 2312 50-51 0.7633867787210136 2312 52-53 0.9464139126139557 2312 54-55 0.8502854070744803 2312 56-57 0.8527053013482018 2312 58-59 0.9139458453690423 2312 60-61 0.9656005082954238 2312 62-63 0.9649042506544063 2312 64-65 0.9516968342010159 2312 66-67 0.9796457452040102 2312 68-69 1.03334504735313 2312 70-71 0.9466918966825304 2312 72-73 0.9140242515476515 2312 74-75 0.9892389018532697 2312 76-77 1.0203935270325424 2312 78-79 0.9215360620549262 2312 80-81 0.8678802973454154 2312 82-83 0.9036166569099109 2312 84-85 0.9882446364824666 2312 86-87 0.9863946173407676 2312 88-89 0.9538616676093774 2312 90-91 1.0486798882763821 2312 92-93 1.1299677131905845 2312 94-95 1.170113119141515 2312 96-97 0.8575276562050611 2312 98-99 0.6673462628826243 2312 100 0.839378249349334 2313 1 0.03775608333761937 2313 2 0.24251745011074632 2313 3 0.29458072518191614 2313 4 0.1894576548708926 2313 5 0.2990365961349255 2313 6 0.23422583281418952 2313 7 0.24242690676804557 2313 8 0.3879283183835298 2313 9 0.5854595479475861 2313 10-11 0.35551393048817914 2313 12-13 0.44524107321837647 2313 14-15 0.4933904336653683 2313 16-17 0.7175137839101708 2313 18-19 0.6607467823957194 2313 20-21 0.6725082072573727 2313 22-23 0.6719581510485568 2313 24-25 0.5465213936540323 2313 26-27 0.7767805057706454 2313 28-29 0.5834840397031797 2313 30-31 0.8071888025654061 2313 32-33 0.9039337999110941 2313 34-35 0.9484787181366983 2313 36-37 0.9746615990868719 2313 38-39 0.9707428293349949 2313 40-41 1.0206768601049703 2313 42-43 1.044185611294985 2313 44-45 0.9462939352236788 2313 46-47 0.9114649523885561 2313 48-49 1.039192311060411 2313 50-51 0.9753580929169985 2313 52-53 0.9689345706443717 2313 54-55 0.8949922203385938 2313 56-57 0.6070473557253493 2313 58-59 0.722750346103922 2313 60-61 0.61565042688175 2313 62-63 0.9549151602756822 2313 64-65 1.071322701799815 2313 66-67 1.01142017613833 2313 68-69 1.1180951377420953 2313 70-71 1.0459655365706482 2313 72-73 1.0844227695663804 2313 74-75 1.1846632042927183 2313 76-77 1.1210063685089757 2313 78-79 0.9951279329634914 2313 80-81 0.8652181276565258 2313 82-83 0.8820124122841904 2313 84-85 0.9303813355926316 2313 86-87 0.9425097925583934 2313 88-89 0.9161376267255825 2313 90-91 0.8009980156503786 2313 92-93 1.0243728053560268 2313 94-95 1.1678157096441488 2313 96-97 1.2010662763817557 2313 98-99 1.2238667909799155 2313 100 1.2542506549509937 2314 1 -0.3130773041682282 2314 2 0.050346204228489455 2314 3 0.27436070664735723 2314 4 0.22656570410086374 2314 5 0.3428014400349255 2314 6 0.3835694823588156 2314 7 0.4254692013949892 2314 8 0.4409108052901516 2314 9 0.46633578591561786 2314 10-11 0.5415721226076187 2314 12-13 0.4078906334635022 2314 14-15 0.7178606264338683 2314 16-17 0.7827233862032514 2314 18-19 0.7859925901986742 2314 20-21 0.8077086720323834 2314 22-23 0.8791817981339989 2314 24-25 0.78587591108775 2314 26-27 0.6971352028555557 2314 28-29 0.7456045454628466 2314 30-31 0.7782728822196745 2314 32-33 0.8038955515933353 2314 34-35 0.8326443145811737 2314 36-37 0.7998210867609927 2314 38-39 0.5632422472953778 2314 40-41 0.5501812609291932 2314 42-43 0.7558136731131313 2314 44-45 0.8445516462407596 2314 46-47 0.7818768550231496 2314 48-49 0.7808296647253243 2314 50-51 0.7575088527684599 2314 52-53 0.7988941214105765 2314 54-55 0.7101024853505535 2314 56-57 0.7078883651785759 2314 58-59 0.7625772258525245 2314 60-61 0.8586442824583216 2314 62-63 0.7422157896595607 2314 64-65 0.7688593419496641 2314 66-67 0.46454427063837755 2314 68-69 0.45183523739937925 2314 70-71 0.6439569140694559 2314 72-73 0.742490415042262 2314 74-75 0.8612148910485757 2314 76-77 0.7211069782143689 2314 78-79 0.5876328626626801 2314 80-81 0.6728279397610422 2314 82-83 0.7040930111651633 2314 84-85 0.7656552419250708 2314 86-87 0.801405436634365 2314 88-89 0.7917206112130906 2314 90-91 0.8575373063481777 2314 92-93 0.7817217484375867 2314 94-95 0.8645304865465988 2314 96-97 0.8044272055739938 2314 98-99 0.9088166354165956 2314 100 0.9209662658582651 2315 1 0.44161338151303653 2315 2 0.47576283000940833 2315 3 0.49884663649831396 2315 4 0.30370893505627805 2315 5 0.372230456923468 2315 6 0.4415431573879829 2315 7 0.3730767036573326 2315 8 0.29712380368743396 2315 9 0.49438981506988 2315 10-11 0.5826545319230263 2315 12-13 0.42364909136208695 2315 14-15 0.6513798536386943 2315 16-17 0.7920257117846461 2315 18-19 0.7390702649443668 2315 20-21 0.49794011262812177 2315 22-23 0.8493852183631816 2315 24-25 0.9068382389587555 2315 26-27 0.7944449887232636 2315 28-29 0.8346582720625761 2315 30-31 0.8662085198738083 2315 32-33 0.7924709830742742 2315 34-35 0.8664395887337477 2315 36-37 0.8445567740231681 2315 38-39 0.9134528089020222 2315 40-41 0.8781262614198226 2315 42-43 0.9345003410881461 2315 44-45 0.9398285245320608 2315 46-47 0.9078995438880568 2315 48-49 0.7935852687986369 2315 50-51 0.8798305636462445 2315 52-53 0.9336321527890235 2315 54-55 0.787093893891857 2315 56-57 0.6883986738387264 2315 58-59 0.7216933849907576 2315 60-61 0.8405122705523951 2315 62-63 0.9300992298409838 2315 64-65 0.9546137249019395 2315 66-67 0.9882959605826613 2315 68-69 1.025325326693853 2315 70-71 0.9443438295395268 2315 72-73 1.0323455023197141 2315 74-75 0.48881324853175556 2315 76-77 0.48009257552790885 2315 78-79 0.7613833941774146 2315 80-81 0.8204586787573263 2315 82-83 0.7700034277636831 2315 84-85 0.7845509996197713 2315 86-87 0.8208735948131469 2315 88-89 0.8449704831044151 2315 90-91 0.8519959245188886 2315 92-93 0.9312826354947994 2315 94-95 0.9539552513172573 2315 96-97 1.0798434906403394 2315 98-99 1.1152572992921286 2315 100 1.0432225595378704 2316 1 0.2951994228723862 2316 2 0.1271299197228828 2316 3 0.25372332344927884 2316 4 0.02712536567021573 2316 5 0.27704010825986813 2316 6 0.36243418783234205 2316 7 0.42706115049870874 2316 8 0.4521481145982804 2316 9 0.6275691400990908 2316 10-11 0.6228456590285987 2316 12-13 0.4110995426904225 2316 14-15 0.48679117901735935 2316 16-17 0.7596848079631116 2316 18-19 0.7315437951171191 2316 20-21 0.6924103184616186 2316 22-23 0.7314322183374173 2316 24-25 0.41012735230774666 2316 26-27 0.6963879014851173 2316 28-29 0.8936483248062217 2316 30-31 0.5505972861160799 2316 32-33 0.673976153833074 2316 34-35 0.8113932126331491 2316 36-37 0.8711310469079621 2316 38-39 0.7289659253692662 2316 40-41 0.7156052182851766 2316 42-43 0.6601991747692608 2316 44-45 0.571956224857658 2316 46-47 0.7402282950543153 2316 48-49 0.8675855868957925 2316 50-51 0.837727605454738 2316 52-53 0.8107386223698612 2316 54-55 0.6639532221468372 2316 56-57 0.7772612973113766 2316 58-59 0.6839769193189369 2316 60-61 0.7990940052795281 2316 62-63 0.8079347467320517 2316 64-65 0.8535233461087088 2316 66-67 0.8508908717002726 2316 68-69 0.9116016747145395 2316 70-71 0.8654551198603073 2316 72-73 0.8250002022611298 2316 74-75 0.8671656732944939 2316 76-77 0.7440495360872141 2316 78-79 0.4945070522050514 2316 80-81 0.4765441908881414 2316 82-83 0.3875482217789781 2316 84-85 0.5222762577656859 2316 86-87 0.5562522704313402 2316 88-89 0.5216409691770174 2316 90-91 0.4099021508226173 2316 92-93 0.2334476002296917 2316 94-95 0.33014321537227787 2316 96-97 0.5365850781467358 2316 98-99 0.6604928423926175 2316 100 0.37080673487660576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 33.0 13 177.0 14 776.0 15 2156.0 16 5570.0 17 11831.0 18 20684.0 19 31729.0 20 47019.0 21 67818.0 22 83329.0 23 81534.0 24 76864.0 25 78934.0 26 90886.0 27 112003.0 28 138803.0 29 171577.0 30 212186.0 31 266635.0 32 340178.0 33 452972.0 34 659882.0 35 922908.0 36 1459343.0 37 2134319.0 38 1804841.0 39 337230.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.18802349090209 22.18011599738759 14.056499938852879 22.575360572857445 2 14.908185512610297 20.382994098268014 41.88124066101812 22.82757972810357 3 18.46395588635059 27.118515171713465 29.460887618243486 24.956641323692455 4 11.955282447217783 17.1113870503061 40.39932987781417 30.53400062466195 5 12.434876157674838 39.02995579708961 35.09030079597281 13.444867249262737 6 29.083983657126318 39.218527416488705 17.898126591001514 13.799362335383458 7 26.505227166304607 32.63703671183259 23.31796083451144 17.539775287351357 8 25.920597470127483 36.04363509404601 19.689504958405738 18.34626247742077 9 25.95484371595752 15.654030723053653 22.177732247345116 36.213393313643714 10-11 25.467777100745852 26.881467615639554 27.201835955222403 20.44891932839219 12-13 25.46054848799109 24.495780727563435 28.210671338893555 21.832999445551913 14-15 22.856974916636304 25.697137948226988 26.123193353063375 25.32269378207334 16-17 20.994938377225232 28.47539024098821 27.418388244521942 23.111283137264614 18-19 20.44452487411133 28.248410389936275 30.159214328613356 21.147850407339043 20-21 22.06163221197923 27.33174421734737 29.666215420146923 20.940408150526476 22-23 22.294692727404566 27.1643628560725 29.260804661711816 21.28013975481112 24-25 21.63115848933215 27.147523683186336 30.215708184984518 21.005609642496996 26-27 21.615546016647677 27.41336371968095 29.20076716996916 21.770323093702217 28-29 20.82221773951502 27.73659998541444 29.83765918624438 21.603523088826158 30-31 21.8091871232813 27.32714771630695 29.71484325968829 21.148821900723462 32-33 22.140134457292973 27.631101687437777 28.838672266487315 21.390091588781935 34-35 22.003455345730988 27.50002016182007 29.593951349918402 20.90257314253054 36-37 21.658372927102786 27.06633583286781 29.357376887451014 21.91791435257839 38-39 21.76369083654991 27.25077454856415 29.887876460154374 21.097658154731565 40-41 21.454163342742277 26.385176566447488 30.44543603853963 21.71522405227061 42-43 21.57696423632606 26.569024630904707 29.647267417572788 22.206743715196442 44-45 21.650488901725854 27.467429961958707 29.428161130622048 21.453920005693398 46-47 21.691249479929425 27.32141893722313 28.72788955425666 22.259442028590783 48-49 22.47158549295031 27.262446033959296 28.712672431887505 21.553296041202888 50-51 22.10255660595265 27.07203485940332 28.59454822404741 22.23086031059662 52-53 21.461151772648222 27.711036672377027 28.319275926953857 22.508535628020894 54-55 21.53692696386112 27.664428359876613 28.222496413850262 22.576148262412 56-57 22.44688648651854 27.189844135373733 27.92394618121236 22.43932319689537 58-59 21.363019343301385 27.698047566436706 28.69583239391471 22.2431006963472 60-61 21.57501358614301 28.32925267864328 27.948377702422604 22.147356032791098 62-63 21.99045165800748 27.500811473823727 27.62990863637026 22.878828231798536 64-65 21.183248688702022 27.702231287986013 27.959708808592126 23.15481121471984 66-67 21.62140259503404 27.38405851149129 27.990847547097964 23.003691346376705 68-69 21.290682016344007 27.39641965778014 28.17455781820059 23.138340507675256 70-71 21.27979324740952 26.979322815318856 28.375708444206843 23.365175493064783 72-73 21.842504566973194 27.140927245825946 28.447360257489702 22.56920792971116 74-75 21.979560857537315 27.56331736119885 27.95530105784083 22.501820723423005 76-77 21.70065936314238 27.47514841641546 27.627778243477486 23.19641397696467 78-79 21.56140271196835 27.518548594735165 27.702675389590333 23.21737330370615 80-81 21.10520301188045 28.021590833960968 28.10227010452257 22.77093604963601 82-83 22.11567046920868 26.951374500268173 29.09185313859453 21.841101891928613 84-85 22.281193006644855 27.43435122731579 27.78014897256023 22.504306793479124 86-87 20.986176762006 28.092696487253587 28.270109394129744 22.651017356610666 88-89 21.911548040978154 26.997843320728066 28.0848394483665 23.005769189927282 90-91 21.60051413897802 27.073581639547108 28.232694621785292 23.093209599689576 92-93 21.922403294488717 27.35145943233795 27.98128785192931 22.744849421244023 94-95 20.82285358027001 27.25573725836654 28.63132872950797 23.290080431855483 96-97 21.203571691637755 27.26683212795451 28.140008714183516 23.389587466224214 98-99 20.55841955508494 27.802321390070283 28.058967936247097 23.58029111859768 100 21.217784488182797 27.261322195393088 27.392241697799076 24.12865161862504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 910.0 1 617.0 2 438.5 3 603.5 4 551.0 5 526.5 6 876.0 7 1686.0 8 2684.0 9 3440.5 10 3558.5 11 3628.0 12 4372.0 13 5446.0 14 6730.0 15 8308.0 16 9939.5 17 11545.0 18 13215.5 19 14749.5 20 16263.0 21 18005.5 22 20144.0 23 23395.5 24 27008.0 25 31066.0 26 38033.0 27 46554.5 28 56092.0 29 74376.5 30 101692.5 31 127911.5 32 164501.5 33 205705.5 34 237659.5 35 269678.5 36 300174.5 37 344198.5 38 361918.5 39 342409.0 40 316058.5 41 289415.5 42 281639.0 43 288153.0 44 306612.0 45 354762.5 46 393916.0 47 413967.0 48 430790.0 49 419305.5 50 394193.0 51 393017.5 52 387130.0 53 356730.0 54 333252.0 55 286703.0 56 241874.0 57 193447.0 58 146731.0 59 141406.0 60 115621.0 61 73007.0 62 50497.0 63 42953.5 64 30779.0 65 12107.0 66 5417.5 67 3435.0 68 2589.5 69 2026.0 70 1452.0 71 980.0 72 668.5 73 464.5 74 356.5 75 242.0 76 142.5 77 82.5 78 54.5 79 41.5 80 27.5 81 15.5 82 8.0 83 7.0 84 7.0 85 6.0 86 3.5 87 1.5 88 2.0 89 3.0 90 2.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04460968244418614 2 0.0 3 0.0 4 0.04021945716633014 5 0.0 6 0.0 7 7.90656685111508E-4 8 0.0 9 0.011537345576166612 10-11 4.7855536204117593E-4 12-13 1.5605066153516608E-5 14-15 1.6125235025300495E-4 16-17 3.068996343524933E-4 18-19 0.0 20-21 5.2016887178388685E-6 22-23 0.0 24-25 6.658161558833752E-4 26-27 5.357739379374034E-4 28-29 1.664540389708438E-4 30-31 0.002429188631230752 32-33 0.015386595227367374 34-35 0.02616969593944735 36-37 0.03128295594908296 38-39 0.022356858109271457 40-41 0.0013628424440737836 42-43 9.88320856389385E-5 44-45 0.011745413124880167 46-47 0.018606440543709635 48-49 0.0032822655809563264 50-51 0.01126685776283899 52-53 0.011214840875660602 54-55 0.010195309886964184 56-57 4.525469184519816E-4 58-59 0.0085515762521271 60-61 0.0046555114024657875 62-63 0.0012796154245883617 64-65 0.036026896059752005 66-67 0.06113544750076023 68-69 0.06041241276898063 70-71 0.05234459356761254 72-73 0.04600893670928479 74-75 0.03583963526590981 76-77 0.03190195690650578 78-79 0.01554784757762038 80-81 0.0013368340004845894 82-83 0.038851413033538514 84-85 0.052615081380940155 86-87 0.058024837647492576 88-89 0.07027481457800312 90-91 0.04935362255485519 92-93 0.04007901157094849 94-95 0.013212289343310726 96-97 0.003609971970180175 98-99 0.022460891883628237 100 0.034383162424914925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9612263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.54592913419524 #Duplication Level Percentage of deduplicated Percentage of total 1 74.02306857507233 45.558185328170765 2 15.49374933479756 19.071543971648705 3 5.374652670030269 9.923639770517886 4 2.2543274006231546 5.54978697776109 5 1.118551435524179 3.442114369186174 6 0.5924699214441247 2.1878467079605395 7 0.3330439332737772 1.4348248821089078 8 0.20767609465310918 1.0225294563509344 9 0.13318826315408158 0.7377475865028889 >10 0.4209357890362524 4.1264956244845985 >50 0.023017837196368466 0.9990532805624671 >100 0.021723671729048603 2.7324142580782858 >500 0.0024586440062939266 1.0342349012286403 >1k 0.0010436597097965169 0.9984378449196338 >5k 2.319243737244101E-5 0.09801956534587132 >10k+ 6.957731211732302E-5 1.0831254751725734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32266 0.3356753763395779 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26946 0.28032940838177234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16669 0.17341389847531222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.0806754871355474E-5 0.0 10-11 0.0 0.0 0.0 3.1210132307033216E-5 0.0 12-13 0.0 0.0 0.0 3.1210132307033216E-5 0.0 14-15 0.0 1.0403377435677737E-5 0.0 3.1210132307033216E-5 0.0 16-17 0.0 2.0806754871355474E-5 0.0 4.681519846054982E-5 0.0 18-19 0.0 2.0806754871355474E-5 0.0 6.76219533319053E-5 0.0 20-21 0.0 2.0806754871355474E-5 0.0 8.842870820326078E-5 0.0 22-23 0.0 2.0806754871355474E-5 0.0 2.548827471741046E-4 0.0 24-25 0.0 2.0806754871355474E-5 0.0 7.490431753687971E-4 0.0 26-27 0.0 2.0806754871355474E-5 0.0 0.001581313370223016 0.0 28-29 0.0 2.0806754871355474E-5 0.0 0.0047647468655404035 0.0 30-31 0.0 2.0806754871355474E-5 0.0 0.0168222613134909 0.0 32-33 0.0 2.0806754871355474E-5 0.0 0.04578526409441772 0.0 34-35 0.0 2.0806754871355474E-5 0.0 0.08856915379864241 0.0 36-37 0.0 2.0806754871355474E-5 0.0 0.16233950319503326 0.0 38-39 0.0 2.0806754871355474E-5 0.0 0.2650156367964547 0.0 40-41 0.0 2.0806754871355474E-5 0.0 0.37199356696752883 0.0 42-43 0.0 2.0806754871355474E-5 0.0 0.4780820083678526 0.0 44-45 0.0 2.0806754871355474E-5 0.0 0.603905656763657 0.0 46-47 0.0 2.0806754871355474E-5 0.0 0.7532201314092217 0.0 48-49 0.0 2.0806754871355474E-5 0.0 0.9169276787370466 0.0 50-51 0.0 2.0806754871355474E-5 0.0 1.0872933876237054 0.0 52-53 0.0 2.0806754871355474E-5 0.0 1.2499970090289874 0.0 54-55 0.0 2.0806754871355474E-5 0.0 1.4254863813027172 0.0 56-57 0.0 2.0806754871355474E-5 0.0 1.6309166738363277 0.0 58-59 0.0 2.0806754871355474E-5 0.0 1.8703243970748615 0.0 60-61 0.0 2.0806754871355474E-5 0.0 2.109170337931869 0.0 62-63 0.0 2.0806754871355474E-5 0.0 2.350247803248829 0.0 64-65 0.0 2.0806754871355474E-5 0.0 2.5969066805600303 0.0 66-67 0.0 2.0806754871355474E-5 0.0 2.8674881242845727 0.0 68-69 5.2016887178388685E-6 2.0806754871355474E-5 0.0 3.1682549676387337 0.0 70-71 1.0403377435677737E-5 2.0806754871355474E-5 0.0 3.4974906533456274 0.0 72-73 1.0403377435677737E-5 2.0806754871355474E-5 0.0 3.830372722843726 0.0 74-75 1.0403377435677737E-5 2.0806754871355474E-5 0.0 4.143103450248917 0.0 76-77 1.0403377435677737E-5 2.0806754871355474E-5 0.0 4.494805229528156 0.0 78-79 1.0403377435677737E-5 2.0806754871355474E-5 0.0 4.874127975899119 0.0 80-81 1.0403377435677737E-5 2.0806754871355474E-5 0.0 5.282913087167923 0.0 82-83 1.0403377435677737E-5 2.0806754871355474E-5 0.0 5.699089798104775 0.0 84-85 1.0403377435677737E-5 2.0806754871355474E-5 0.0 6.094829074069239 0.0 86-87 1.0403377435677737E-5 2.0806754871355474E-5 0.0 6.509429673324585 0.0 88 1.0403377435677737E-5 2.0806754871355474E-5 0.0 6.844454838574434 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19980 0.0 47.33181 1 GTATCAA 35175 0.0 38.944206 1 GGGCACC 9720 0.0 35.30691 94 GTACTAG 9730 0.0 34.12152 1 CAACGCA 40015 0.0 32.563007 5 ATCAACG 40190 0.0 32.412167 3 TACCTGG 4740 0.0 30.840212 2 TATCAAC 44855 0.0 30.399902 2 ACCTGGG 4795 0.0 30.000076 3 TAGGCAT 12045 0.0 29.882671 5 GTGGTAT 6360 0.0 29.871758 1 CTGTGCG 12375 0.0 29.693647 9 AACGCAG 45740 0.0 29.247503 6 CTAGGCA 13520 0.0 27.816525 4 ACTGTGC 14230 0.0 27.737791 8 TAGTACT 13670 0.0 27.580074 4 TCAACGC 48305 0.0 27.414753 4 GCATCGT 11320 0.0 27.188944 8 ACGCAGA 48805 0.0 26.707893 7 CGCAGAG 48910 0.0 26.612125 8 >>END_MODULE