FastQCFastQC Report
Mon 6 Jun 2016
ERR1211233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824343
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189320.3250495377761921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150270.2580033490472659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93140.1599150324766244No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95850.047.4253161
GTATCAA169350.040.374361
TACCTGG41750.033.4756432
CAACGCA198750.033.2061585
ATCAACG199500.033.0813263
TATCAAC216850.031.2704072
GTACTAG36900.030.3511961
AACGCAG221150.030.2253466
TCAACGC222000.030.0460974
GTACATG224450.027.9680041
ACCTGGG50700.027.53643
ACGCAGA240150.027.3446567
TACATGG226400.027.249292
TCTAACG16950.027.2072932
GTACCTG53400.027.1415521
CGCAGAG244300.026.7823288
CTAACGC17850.026.3342193
CTGTGCG50450.026.2732039
ACATGGG225600.026.1911683
GTGGTAT37500.026.101011
Produced by FastQC (version 0.11.5)