##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211233_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5824343 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.264438409619764 31.0 31.0 34.0 28.0 34.0 2 31.274261320117994 31.0 31.0 34.0 28.0 34.0 3 31.333670767672853 31.0 31.0 34.0 28.0 34.0 4 34.93241520974984 37.0 35.0 37.0 32.0 37.0 5 34.98797890165466 37.0 35.0 37.0 32.0 37.0 6 35.05057514641565 37.0 35.0 37.0 32.0 37.0 7 34.95565903313043 37.0 35.0 37.0 32.0 37.0 8 34.962276088478994 37.0 35.0 37.0 32.0 37.0 9 36.501481111260105 39.0 37.0 39.0 32.0 39.0 10-11 36.44596884146418 39.0 37.0 39.0 32.0 39.0 12-13 36.33055058742248 39.0 37.0 39.0 32.0 39.0 14-15 37.491814613253375 40.0 37.0 41.0 32.0 41.0 16-17 37.46523470544231 40.0 37.0 41.0 31.5 41.0 18-19 37.48040817651021 40.0 37.0 41.0 31.0 41.0 20-21 37.44695899606187 40.0 37.0 41.0 31.0 41.0 22-23 37.335981157016334 40.0 37.0 41.0 31.0 41.0 24-25 37.23894935445938 40.0 36.5 41.0 31.0 41.0 26-27 37.087901502366876 40.0 36.0 41.0 30.0 41.0 28-29 36.96403834732948 39.5 36.0 41.0 30.0 41.0 30-31 36.80765779075855 39.0 36.0 41.0 30.0 41.0 32-33 36.61853903521822 39.0 35.0 41.0 30.0 41.0 34-35 36.4641957041335 39.0 35.0 41.0 29.5 41.0 36-37 36.35592829955242 39.0 35.0 41.0 29.0 41.0 38-39 36.21536609365211 39.0 35.0 40.0 28.5 41.0 40-41 36.051689606879265 39.0 35.0 40.0 28.0 41.0 42-43 35.83895230758216 38.0 35.0 40.0 27.0 41.0 44-45 35.62387474432739 38.0 34.0 40.0 26.5 41.0 46-47 35.3843019719134 38.0 34.0 40.0 26.0 41.0 48-49 35.17506438065203 38.0 34.0 40.0 25.0 41.0 50-51 34.49932842210701 37.5 32.5 39.5 24.0 40.5 52-53 34.61335175486746 37.5 33.0 39.5 24.5 40.5 54-55 35.102687719456085 38.0 34.0 40.0 26.0 41.0 56-57 35.065494940802765 38.0 34.0 40.0 26.0 41.0 58-59 34.856668211333016 37.5 34.0 40.0 25.5 41.0 60-61 34.63284039075309 37.0 33.0 40.0 25.0 41.0 62-63 34.33235233570551 36.5 33.0 40.0 25.0 41.0 64-65 34.0845187173901 36.0 33.0 39.5 24.5 41.0 66-67 33.91055102695703 36.0 33.0 39.0 24.0 41.0 68-69 33.618924743958246 35.0 32.5 39.0 23.5 41.0 70-71 33.31951818428276 35.0 32.0 38.5 23.5 40.5 72-73 32.986328071681214 35.0 32.0 37.5 23.0 40.0 74-75 32.65523931197046 35.0 31.5 37.0 22.5 39.5 76-77 32.32417227144761 35.0 31.0 36.5 22.0 39.0 78-79 31.99463261349821 35.0 31.0 36.0 21.0 39.0 80-81 31.705959453280826 35.0 31.0 36.0 20.0 37.5 82-83 31.419850273241117 34.5 31.0 35.0 20.0 37.0 84-85 31.173247866755098 34.0 30.0 35.0 20.0 37.0 86-87 30.950999365937065 34.0 30.0 35.0 19.5 36.0 88-89 30.74038548210502 34.0 30.0 35.0 19.0 36.0 90-91 30.51633403115167 34.0 29.5 35.0 18.5 36.0 92-93 30.32494308800151 34.0 29.0 35.0 18.0 35.0 94-95 30.155681507768342 34.0 29.0 35.0 18.0 35.0 96-97 29.97660276875864 34.0 29.0 35.0 17.5 35.0 98-99 29.743364616403944 34.0 29.0 35.0 16.5 35.0 100 28.60248460641827 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5856402828995435 1101 2 -0.49362562054396975 1101 3 -0.5127108617350977 1101 4 -0.4602704499256376 1101 5 -0.44052959710338513 1101 6 -0.42056354245049477 1101 7 -0.4437631767563843 1101 8 -0.4019957780774206 1101 9 -0.713375607525407 1101 10-11 -0.6214484240664646 1101 12-13 -0.6988363104137107 1101 14-15 -0.8563543680487911 1101 16-17 -0.8800823506263882 1101 18-19 -0.8283627015697093 1101 20-21 -0.8394714655620348 1101 22-23 -0.9235070719411738 1101 24-25 -1.0492569296494452 1101 26-27 -0.9047747622715576 1101 28-29 -0.947063449390761 1101 30-31 -0.9976130603682094 1101 32-33 -1.0562741845534092 1101 34-35 -1.100625398074456 1101 36-37 -1.1385357341555178 1101 38-39 -1.1518329560416305 1101 40-41 -1.1783779354081219 1101 42-43 -1.1848104905571972 1101 44-45 -1.1871866261553592 1101 46-47 -1.2834186116260256 1101 48-49 -1.2876579092005542 1101 50-51 -1.3691246791970215 1101 52-53 -1.3322826453245469 1101 54-55 -1.1559568994296683 1101 56-57 -1.0512267417600185 1101 58-59 -1.0280364746644892 1101 60-61 -0.9894921979651556 1101 62-63 -1.032597601116123 1101 64-65 -0.9887499898183236 1101 66-67 -1.0428126838318406 1101 68-69 -1.0896033495746096 1101 70-71 -1.136319919624306 1101 72-73 -1.1442742682056561 1101 74-75 -1.2498870813855163 1101 76-77 -1.1920922632646587 1101 78-79 -1.228586710860153 1101 80-81 -1.3334584631120023 1101 82-83 -1.2190713273473683 1101 84-85 -1.2111541834416926 1101 86-87 -1.308571746758254 1101 88-89 -1.3821400119178868 1101 90-91 -1.5033378212824822 1101 92-93 -1.4608183612654564 1101 94-95 -1.574960343241191 1101 96-97 -1.555041023333029 1101 98-99 -1.7282706265083654 1101 100 -1.8322169611063366 1102 1 -0.40183466574174886 1102 2 -0.36965121056993766 1102 3 -0.42979172628157514 1102 4 -0.3029424678002215 1102 5 -0.3576045564244339 1102 6 -0.35865208540054283 1102 7 -0.38794344288879046 1102 8 -0.46849581001931995 1102 9 -0.615459668946194 1102 10-11 -0.5001444107142063 1102 12-13 -0.5831611042542448 1102 14-15 -0.6512968645741068 1102 16-17 -0.757667671739128 1102 18-19 -0.8284557357121187 1102 20-21 -0.8048922907152871 1102 22-23 -0.8113583053548723 1102 24-25 -0.7746577736672151 1102 26-27 -0.8852833847059358 1102 28-29 -0.8475830018605635 1102 30-31 -0.8032113751242349 1102 32-33 -0.9772033771426791 1102 34-35 -1.0318923856632765 1102 36-37 -0.9767085070936901 1102 38-39 -1.0335807095059835 1102 40-41 -1.1663606406133624 1102 42-43 -1.0573273030024097 1102 44-45 -1.3172775075746799 1102 46-47 -1.253663147010947 1102 48-49 -1.2530152263385403 1102 50-51 -1.23464498055678 1102 52-53 -1.0619769103306638 1102 54-55 -0.8663686707986713 1102 56-57 -0.9163068890900234 1102 58-59 -0.9458248925848096 1102 60-61 -0.9248326637371278 1102 62-63 -1.0185904064042006 1102 64-65 -0.9960253349116428 1102 66-67 -1.089330506607432 1102 68-69 -1.0859817285043363 1102 70-71 -1.1500293299231288 1102 72-73 -1.1871865750712338 1102 74-75 -1.1603629512923241 1102 76-77 -1.2512851692636708 1102 78-79 -1.2108760271799959 1102 80-81 -1.3133955670928223 1102 82-83 -1.2746124956264282 1102 84-85 -1.3034140434234267 1102 86-87 -1.391602061501402 1102 88-89 -1.3819752561284844 1102 90-91 -1.4560239204355092 1102 92-93 -1.4883701031190633 1102 94-95 -1.5282321333888085 1102 96-97 -1.6624145562200354 1102 98-99 -1.7073123909467718 1102 100 -1.702470783800333 1103 1 -0.5681529463457444 1103 2 -0.4536223612077528 1103 3 -0.5201051689759701 1103 4 -0.41549594225845965 1103 5 -0.35204714134320625 1103 6 -0.4844199254540982 1103 7 -0.4083469966256743 1103 8 -0.46498795889242217 1103 9 -0.6184685386217339 1103 10-11 -0.5760263104056307 1103 12-13 -0.7110407368957823 1103 14-15 -0.8629813905443413 1103 16-17 -0.9099537097394546 1103 18-19 -0.7860419980602771 1103 20-21 -0.8415281940178545 1103 22-23 -0.8956051896017527 1103 24-25 -0.847613251384594 1103 26-27 -0.8839643786250306 1103 28-29 -0.9710127110045619 1103 30-31 -0.9222721941850551 1103 32-33 -1.019247200138885 1103 34-35 -1.0810667824567233 1103 36-37 -0.9955196259048122 1103 38-39 -1.0575970859033106 1103 40-41 -1.1100477081099527 1103 42-43 -1.219884627464495 1103 44-45 -1.1084056844171428 1103 46-47 -1.1233333928716647 1103 48-49 -1.0719449881254448 1103 50-51 -1.1416218161050438 1103 52-53 -1.0661363811568023 1103 54-55 -0.8095829473462786 1103 56-57 -0.772033259102102 1103 58-59 -0.7752869553802029 1103 60-61 -0.955662813614893 1103 62-63 -0.8230646870689569 1103 64-65 -0.7824698642133114 1103 66-67 -0.8843891445232188 1103 68-69 -0.7138513039929606 1103 70-71 -0.8643828252290078 1103 72-73 -0.9088703729455077 1103 74-75 -0.9699401370599858 1103 76-77 -0.9107429001499732 1103 78-79 -1.010201726505695 1103 80-81 -1.0579452449758335 1103 82-83 -1.014311168658434 1103 84-85 -1.0494264608644173 1103 86-87 -1.0433995418703113 1103 88-89 -1.1097707124953686 1103 90-91 -1.2230832874948767 1103 92-93 -1.2573324720814298 1103 94-95 -1.4491764162378509 1103 96-97 -1.4940640497742912 1103 98-99 -1.300975494133688 1103 100 -1.5592282215101498 1104 1 -0.5684084439253212 1104 2 -0.5971416223086159 1104 3 -0.6477053248455213 1104 4 -0.4443350482753914 1104 5 -0.4464070382412544 1104 6 -0.438645554674423 1104 7 -0.5979233364245715 1104 8 -0.47752466216142864 1104 9 -0.6955265472450236 1104 10-11 -0.7206830864210403 1104 12-13 -0.6277324998147549 1104 14-15 -0.8072598507856057 1104 16-17 -0.9439760784110334 1104 18-19 -0.9022811384754519 1104 20-21 -0.7573349901102588 1104 22-23 -0.8573414860932473 1104 24-25 -0.8771699192678852 1104 26-27 -0.9012173701255861 1104 28-29 -0.9123713450428212 1104 30-31 -0.9943194427901716 1104 32-33 -1.093656122020242 1104 34-35 -1.1326183036987914 1104 36-37 -1.111298423990732 1104 38-39 -1.0673817259603098 1104 40-41 -1.0600171356917159 1104 42-43 -1.0743554668468391 1104 44-45 -1.0825765260636047 1104 46-47 -1.1791517714300852 1104 48-49 -1.1048030918689875 1104 50-51 -1.1158924971900532 1104 52-53 -0.9522711382114366 1104 54-55 -0.802971312396366 1104 56-57 -0.9512837356581159 1104 58-59 -0.8554471813119449 1104 60-61 -0.9154749504352964 1104 62-63 -1.058502688959905 1104 64-65 -0.9567365183045737 1104 66-67 -1.0003296020278967 1104 68-69 -1.055120014340197 1104 70-71 -0.9103775895233639 1104 72-73 -0.862444352059967 1104 74-75 -0.9610322802690234 1104 76-77 -0.9709740932367197 1104 78-79 -1.1012970476640724 1104 80-81 -1.0392313344777975 1104 82-83 -1.0392212790655755 1104 84-85 -1.0535790552167796 1104 86-87 -1.0604676701481672 1104 88-89 -1.1121996785079524 1104 90-91 -1.1645230581260435 1104 92-93 -1.2234513039272663 1104 94-95 -1.2577335085751464 1104 96-97 -1.2782714692085015 1104 98-99 -1.256570198933563 1104 100 -1.1574901082682238 1105 1 -0.572282931809351 1105 2 -0.5567326467198512 1105 3 -0.602350564072033 1105 4 -0.46035744242315246 1105 5 -0.33037186920937245 1105 6 -0.38161410156497055 1105 7 -0.3520056963266711 1105 8 -0.4213560740326514 1105 9 -0.6832144725141944 1105 10-11 -0.6961911682161528 1105 12-13 -0.6581392454018129 1105 14-15 -0.927295575637018 1105 16-17 -0.9111675985774284 1105 18-19 -0.8540374609224912 1105 20-21 -0.856462487936291 1105 22-23 -0.7986212829501795 1105 24-25 -0.8747185983000421 1105 26-27 -0.9255795784323979 1105 28-29 -0.9061794483535337 1105 30-31 -0.9921180164855627 1105 32-33 -1.0547776111683262 1105 34-35 -0.947260898300172 1105 36-37 -1.0553497543716475 1105 38-39 -1.060939654456817 1105 40-41 -1.18266022560001 1105 42-43 -1.0380366953872766 1105 44-45 -1.1391706635123953 1105 46-47 -1.1967211592217737 1105 48-49 -1.132096519751407 1105 50-51 -1.0766380237393989 1105 52-53 -1.0321987696134514 1105 54-55 -0.8625818353149555 1105 56-57 -0.8601553884143556 1105 58-59 -0.8670062102414562 1105 60-61 -0.8710301200050736 1105 62-63 -0.8449976753781883 1105 64-65 -0.7897469309557437 1105 66-67 -0.8559919013277479 1105 68-69 -0.9015874787274072 1105 70-71 -0.872088516307592 1105 72-73 -0.9520279468675383 1105 74-75 -0.9045577672834639 1105 76-77 -0.9353223612313748 1105 78-79 -1.0932455088508384 1105 80-81 -1.042783368597945 1105 82-83 -1.1581635746898442 1105 84-85 -1.057195804210476 1105 86-87 -1.0555784754589332 1105 88-89 -1.1654264013243427 1105 90-91 -1.17113434081406 1105 92-93 -1.2027739212993147 1105 94-95 -1.2737682293654835 1105 96-97 -1.2949729493020925 1105 98-99 -1.2697336524553968 1105 100 -1.146019607044522 1106 1 -0.6188307696113604 1106 2 -0.6260075240463756 1106 3 -0.6963405344373825 1106 4 -0.5975520274853565 1106 5 -0.5761396204646658 1106 6 -0.496546543104067 1106 7 -0.5853034122455796 1106 8 -0.606069783233707 1106 9 -0.7786270710953005 1106 10-11 -0.7751817514635633 1106 12-13 -0.8450207302347934 1106 14-15 -1.000242597529386 1106 16-17 -1.084759716969593 1106 18-19 -1.018871345618571 1106 20-21 -0.9582887690123201 1106 22-23 -1.0196537313114007 1106 24-25 -1.0922864604287525 1106 26-27 -1.094036192344987 1106 28-29 -1.0811828767492244 1106 30-31 -1.0792634323218806 1106 32-33 -1.0676919686654784 1106 34-35 -1.0767769863155792 1106 36-37 -1.087388528036243 1106 38-39 -1.1198875904031524 1106 40-41 -1.1565133353802182 1106 42-43 -1.06088844323817 1106 44-45 -1.1356729546097384 1106 46-47 -1.1234299458151398 1106 48-49 -1.0994464932948702 1106 50-51 -1.0633818319666588 1106 52-53 -1.1324844045454014 1106 54-55 -1.00794394045667 1106 56-57 -0.9928784303393741 1106 58-59 -1.0183026637897044 1106 60-61 -1.0534310313967552 1106 62-63 -1.0130136589817837 1106 64-65 -1.049055545221087 1106 66-67 -1.0724520345976956 1106 68-69 -1.079822941359076 1106 70-71 -1.008463579606378 1106 72-73 -1.0175174628404662 1106 74-75 -0.9337158219403427 1106 76-77 -0.9016567635493473 1106 78-79 -0.9266754138624371 1106 80-81 -0.977960725850501 1106 82-83 -1.0023622820078764 1106 84-85 -1.0320047049496495 1106 86-87 -1.024834761798541 1106 88-89 -1.1403662154740992 1106 90-91 -1.2275638098751607 1106 92-93 -1.1855537380115244 1106 94-95 -1.2196418345041558 1106 96-97 -1.110624126784419 1106 98-99 -1.114382315041361 1106 100 -1.3314603510556111 1107 1 -0.4925551237596544 1107 2 -0.4538044282647071 1107 3 -0.5156916135532548 1107 4 -0.2945754476994793 1107 5 -0.34071439596149133 1107 6 -0.4610921362146527 1107 7 -0.38592201002651905 1107 8 -0.45318129870382506 1107 9 -0.4998192208175354 1107 10-11 -0.5127615379862434 1107 12-13 -0.5458091572262589 1107 14-15 -0.7286034550719549 1107 16-17 -0.7338255011580301 1107 18-19 -0.7252043585370487 1107 20-21 -0.7584852812854379 1107 22-23 -0.8479010197223147 1107 24-25 -0.8060217549362676 1107 26-27 -0.7551531687529547 1107 28-29 -0.8153170370022806 1107 30-31 -0.8094788149944705 1107 32-33 -0.9378471132533761 1107 34-35 -0.8399436408454051 1107 36-37 -0.9506831303977279 1107 38-39 -0.8926417651253047 1107 40-41 -0.9846203952072443 1107 42-43 -0.9283126804241846 1107 44-45 -0.823087369217717 1107 46-47 -0.899562120033444 1107 48-49 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1.2304130280297159 2311 68-69 1.2265276166291201 2311 70-71 1.1644877264378977 2311 72-73 1.1760036101047646 2311 74-75 1.2205885899429063 2311 76-77 1.2745900587392143 2311 78-79 1.282105065801101 2311 80-81 1.3041328724214338 2311 82-83 1.3275412476620723 2311 84-85 1.286146041881338 2311 86-87 1.3392046416457255 2311 88-89 1.3911768872364263 2311 90-91 1.3971791412781904 2311 92-93 1.4239235921533577 2311 94-95 1.4261096110238611 2311 96-97 1.4789810326932837 2311 98-99 1.5120777277341233 2311 100 1.6047975716949736 2312 1 0.5688305999412044 2312 2 0.6055284759501589 2312 3 0.622285419653668 2312 4 0.3683700949136792 2312 5 0.5123823632480224 2312 6 0.5219883858944101 2312 7 0.3791539898729539 2312 8 0.5404885719799992 2312 9 0.6937026917393538 2312 10-11 0.6961383488989341 2312 12-13 0.7983059446312382 2312 14-15 0.9939316855925355 2312 16-17 0.9693598910195718 2312 18-19 0.9855831765825016 2312 20-21 1.0218069339841307 2312 22-23 1.1040025823379693 2312 24-25 1.0574200218048446 2312 26-27 1.0996777802719322 2312 28-29 1.1267666074094436 2312 30-31 1.1171115818585093 2312 32-33 1.1091285764848706 2312 34-35 1.145836018917528 2312 36-37 1.2033153381866413 2312 38-39 1.2526433986127827 2312 40-41 1.2948263431168598 2312 42-43 1.313986057633869 2312 44-45 1.3912924307417214 2312 46-47 1.4297350840091454 2312 48-49 1.334085014550027 2312 50-51 1.315390002040111 2312 52-53 1.2260606233780535 2312 54-55 1.133365845027626 2312 56-57 1.0786133690672628 2312 58-59 1.0605523690351788 2312 60-61 0.9854673522773112 2312 62-63 1.0391938023479739 2312 64-65 1.1231625984861964 2312 66-67 1.1039627458249655 2312 68-69 1.0152189486937928 2312 70-71 1.0476789390164711 2312 72-73 1.0505204787865665 2312 74-75 1.1017933681409176 2312 76-77 1.095429532330158 2312 78-79 1.088886653906389 2312 80-81 1.1111987663704213 2312 82-83 1.0850515342567526 2312 84-85 1.1203946164701364 2312 86-87 1.1448019291183904 2312 88-89 1.103474923278064 2312 90-91 1.1644780651313305 2312 92-93 1.1766286538018669 2312 94-95 1.2858747282956884 2312 96-97 1.2613128555773443 2312 98-99 0.9620468297882319 2312 100 1.192768923280962 2313 1 0.6648682435404858 2313 2 0.6801915228620175 2313 3 0.6802229137910665 2313 4 0.6019902632669343 2313 5 0.4398005833894416 2313 6 0.5735717239982918 2313 7 0.6601894444798546 2313 8 0.6227848137758016 2313 9 0.7565691690669496 2313 10-11 0.8355915719682088 2313 12-13 0.9247862625808807 2313 14-15 1.137411352793272 2313 16-17 1.0828878983967911 2313 18-19 1.0899991152231152 2313 20-21 1.1306023661372677 2313 22-23 1.111680792095683 2313 24-25 1.0880323559966456 2313 26-27 1.104381193358499 2313 28-29 1.1707541786055842 2313 30-31 1.1400946762677933 2313 32-33 1.2197841672007215 2313 34-35 1.255552741836432 2313 36-37 1.2924268232494143 2313 38-39 1.3182084190146384 2313 40-41 1.3236856513435455 2313 42-43 1.3288268375358996 2313 44-45 1.2884588236478365 2313 46-47 1.3603576906878203 2313 48-49 1.3362108438496634 2313 50-51 1.3069538353511447 2313 52-53 1.268694062680801 2313 54-55 1.1408253495308003 2313 56-57 1.034424066144517 2313 58-59 1.0359941312335366 2313 60-61 1.0354017579439017 2313 62-63 1.0641836311634236 2313 64-65 1.1846107354890165 2313 66-67 1.1396277598124485 2313 68-69 1.0903870308053953 2313 70-71 1.1494529561166473 2313 72-73 1.0725417830535733 2313 74-75 1.1160260538923126 2313 76-77 0.8757949798226221 2313 78-79 1.1432372805668827 2313 80-81 1.1462868420607606 2313 82-83 1.1337334983217495 2313 84-85 1.2573220815910986 2313 86-87 1.3150465005207543 2313 88-89 1.368603563437027 2313 90-91 1.3774510090476078 2313 92-93 1.4196354740779178 2313 94-95 1.4304992195424795 2313 96-97 1.511202888670013 2313 98-99 1.4744716677734822 2313 100 1.599717284269687 2314 1 0.6116472110715492 2314 2 0.644100074530737 2314 3 0.6318559688141328 2314 4 0.5496587719952046 2314 5 0.5583783386677865 2314 6 0.5535940527630316 2314 7 0.625518885147315 2314 8 0.6248641129182033 2314 9 0.7371170395842555 2314 10-11 0.7469628739551908 2314 12-13 0.7883100828084935 2314 14-15 1.0208940741592656 2314 16-17 1.030445337618147 2314 18-19 0.9536211666725123 2314 20-21 1.028166338735737 2314 22-23 1.0370526894437404 2314 24-25 1.0220264351681791 2314 26-27 1.0883349823231683 2314 28-29 1.0805224765889463 2314 30-31 1.080097209130443 2314 32-33 1.0750494696820354 2314 34-35 1.0524775229421053 2314 36-37 1.098883837191309 2314 38-39 1.0329359379930878 2314 40-41 1.1663007783773836 2314 42-43 1.250661803046711 2314 44-45 1.1864418378734527 2314 46-47 1.2935809382587493 2314 48-49 1.1656253631306086 2314 50-51 1.179195962097232 2314 52-53 1.163969707894239 2314 54-55 1.0351889420070322 2314 56-57 1.0208479306909481 2314 58-59 0.9989212510241003 2314 60-61 0.9935320647145645 2314 62-63 1.0545030296718423 2314 64-65 1.0839432264072641 2314 66-67 1.0926598420686915 2314 68-69 1.1078620538954524 2314 70-71 1.1142636986235317 2314 72-73 1.047736675558248 2314 74-75 1.124264617275422 2314 76-77 1.1057688899119498 2314 78-79 1.1288383210779322 2314 80-81 1.1723714392542632 2314 82-83 1.1043285433012322 2314 84-85 1.203308271914139 2314 86-87 1.2180963509636378 2314 88-89 1.2614978482391166 2314 90-91 1.285372344168895 2314 92-93 1.298714349232018 2314 94-95 1.344284559306594 2314 96-97 1.3815539517345172 2314 98-99 1.47178777448271 2314 100 1.5885909870683967 2315 1 0.4011862973815141 2315 2 0.4463991436730872 2315 3 0.4401018672266801 2315 4 0.24116194220358977 2315 5 0.2912083590551475 2315 6 0.3407817572558116 2315 7 0.35808750510294374 2315 8 0.39191665175716395 2315 9 0.5247671340257014 2315 10-11 0.435319238389674 2315 12-13 0.4948744924940627 2315 14-15 0.7382776794761412 2315 16-17 0.7085166317853222 2315 18-19 0.5788058212084408 2315 20-21 0.6696296572934486 2315 22-23 0.5162099869193 2315 24-25 0.6951951371490424 2315 26-27 0.7770701879630693 2315 28-29 0.7765730448018715 2315 30-31 0.8265217221280352 2315 32-33 0.828188718999634 2315 34-35 0.8977014189092145 2315 36-37 0.9331897740322148 2315 38-39 0.9643923209052474 2315 40-41 0.9880867669883528 2315 42-43 1.0604416824562577 2315 44-45 1.1255974350445115 2315 46-47 1.1592672697984128 2315 48-49 1.1298645253391655 2315 50-51 1.0729799274517617 2315 52-53 1.0916340125470967 2315 54-55 1.0517012451399594 2315 56-57 0.9059932981718006 2315 58-59 0.9154846529105924 2315 60-61 0.9559415461078373 2315 62-63 0.9481748702502486 2315 64-65 0.89062734176661 2315 66-67 0.9322789249764227 2315 68-69 0.9636571063433692 2315 70-71 0.8691746008750059 2315 72-73 0.6338030223037023 2315 74-75 0.8672088627671286 2315 76-77 0.9813785391033676 2315 78-79 0.9886536323240911 2315 80-81 0.7313664452309965 2315 82-83 0.6733016359620443 2315 84-85 0.9292995771418546 2315 86-87 1.0608585734400506 2315 88-89 1.0372566060701303 2315 90-91 1.0519372573466406 2315 92-93 1.2083812321233225 2315 94-95 1.2295055931895007 2315 96-97 1.0554976080263145 2315 98-99 0.5182114442531045 2315 100 0.957145268739847 2316 1 0.3605348841539602 2316 2 0.28851408247745525 2316 3 0.3515187037242633 2316 4 0.26924646239066163 2316 5 0.2664188143828099 2316 6 0.24160264335984039 2316 7 0.31128120060821374 2316 8 0.3494249624989152 2316 9 0.3462528651955239 2316 10-11 0.393314768485169 2316 12-13 0.24704087005265762 2316 14-15 0.22032437188597243 2316 16-17 0.40226096604635586 2316 18-19 0.46331833005385903 2316 20-21 0.451427292114694 2316 22-23 0.4273249282216227 2316 24-25 0.5410077270827003 2316 26-27 0.5501206533491327 2316 28-29 0.5075098441489772 2316 30-31 0.4255922941147574 2316 32-33 0.6474488652296699 2316 34-35 0.666619589728839 2316 36-37 0.6654207200501787 2316 38-39 0.8261732991527282 2316 40-41 0.8906183033196768 2316 42-43 0.7567832313118359 2316 44-45 0.6880417441238151 2316 46-47 0.4947635014017564 2316 48-49 0.47508293009783387 2316 50-51 0.5448452995613806 2316 52-53 0.674773397844227 2316 54-55 0.41889156555686213 2316 56-57 0.2916157000799515 2316 58-59 0.3910842235485319 2316 60-61 0.4016146849649971 2316 62-63 0.6434704319507674 2316 64-65 0.5284110955942722 2316 66-67 0.4452788556285512 2316 68-69 0.4965349264554604 2316 70-71 0.23551954337515468 2316 72-73 0.11953050467500503 2316 74-75 0.4506389619559954 2316 76-77 0.6226479453559222 2316 78-79 0.6214503166155829 2316 80-81 0.767776248807607 2316 82-83 0.7867892201355495 2316 84-85 0.7719216296937432 2316 86-87 0.8317824203175732 2316 88-89 0.7997171875145668 2316 90-91 0.7233283816041087 2316 92-93 0.741329886982772 2316 94-95 0.8979522579553425 2316 96-97 0.8335497738031776 2316 98-99 0.7259643757470435 2316 100 0.5943567539369319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 23.0 1 350.0 2 146.0 3 128.0 4 113.0 5 71.0 6 107.0 7 423.0 8 1515.0 9 2853.0 10 4560.0 11 7070.0 12 10190.0 13 13395.0 14 16159.0 15 19146.0 16 22738.0 17 27664.0 18 33224.0 19 39770.0 20 48072.0 21 56952.0 22 63362.0 23 65106.0 24 65220.0 25 67509.0 26 74175.0 27 84333.0 28 98571.0 29 117823.0 30 141297.0 31 173762.0 32 218172.0 33 283098.0 34 394681.0 35 541092.0 36 796480.0 37 1105368.0 38 969489.0 39 259824.0 40 312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20283668181444 19.410447893240743 12.890285112926309 23.496430312018507 2 15.776870444230504 19.440052950075643 41.33795213863293 23.445124467060925 3 19.569689989478626 25.36590844187992 28.74449439440957 26.319907174231886 4 12.672703675198088 15.914376000057699 38.21818266741811 33.194737657326094 5 13.393397157944447 37.58943888721934 34.41109346155498 14.606070493281242 6 31.09576795178844 36.82262612297989 17.853701088168332 14.227904837063344 7 27.972716215659055 31.833297829941042 22.10240781715982 18.09157813724008 8 26.24995898816987 35.680927789805054 19.606008151519948 18.463105070505133 9 26.46056538724203 15.881368438843612 20.935394620947605 36.72267155296675 10-11 25.8854774763324 26.03431699442249 27.24139337698067 20.83881215226443 12-13 25.848198646832714 23.57828848497257 27.872607736504335 22.70090513169038 14-15 23.1401365217018 24.989730820418153 25.747786888764868 26.122345769115185 16-17 22.202697215660223 27.743660533461988 26.766242482893936 23.28739976798385 18-19 21.778684481330792 27.326825846374707 29.020876076549477 21.87361359574502 20-21 22.934015489855362 26.59738113786957 28.680403389843022 21.788199982432044 22-23 23.02952360352092 26.38423850728067 28.387278427855637 22.198959461342767 24-25 22.458845135846772 26.30346920872887 29.05631470590862 22.18137094951574 26-27 22.447725285184568 26.599718969072427 28.394281325439053 22.55827442030395 28-29 21.975340421752133 26.96050808014659 28.716476327799175 22.347675170302107 30-31 22.73319870315419 26.632313647104077 28.63633709748324 21.998150552258487 32-33 22.75594442907429 26.776648286021594 28.125066010094844 22.34234127480928 34-35 22.83832211413996 26.718369838824003 28.554198814782634 21.889109232253404 36-37 22.6016024622459 26.582504669218572 28.27242218820564 22.543470680329897 38-39 22.63833615714522 26.70672037461846 28.536545323468708 22.11839814476761 40-41 22.593617602255925 26.094070126689665 28.859689947775074 22.452622323279332 42-43 22.4919370787721 26.3289575682142 28.40493490195297 22.774170451060726 44-45 22.45232442578557 26.924594388756763 28.341661579600252 22.281419605857415 46-47 22.452408595800424 26.701378850818514 27.853521728697 22.992690824684058 48-49 22.919816715873704 26.66090289606714 27.840454799116248 22.578825588942905 50-51 22.7418855957055 26.712706010492383 27.77618153499553 22.76922685880659 52-53 22.443556496577617 27.07290582156061 27.57072114861487 22.9128165332469 54-55 22.356958949582534 26.951865513120126 27.691411787448377 22.999763749848963 56-57 22.8758202490395 26.75758879309375 27.376224184843633 22.990366773023116 58-59 22.395245744626763 26.996730916951 27.863127244145126 22.744896094277113 60-61 22.5486689006931 27.324042082312307 27.474575255337957 22.65271376165664 62-63 22.65738937490769 26.941327398064857 27.331670908597555 23.069612318429904 64-65 22.212397899001168 26.922763502645914 27.561608796517923 23.303229801834995 66-67 22.48845179069701 26.71835405073767 27.407043044414632 23.386151114150692 68-69 22.140502303826107 26.792451598696953 27.62788699658231 23.43915910089463 70-71 22.21817816119446 26.486399258808923 27.734264997967617 23.561157582029 72-73 22.55282061200677 26.659441441234076 27.67846790637771 23.109270040381446 74-75 22.6958732106206 26.806270017357754 27.383633772237527 23.114222999784122 76-77 22.515371874428467 26.706765342036547 27.217332244572727 23.56053053896226 78-79 22.32171368559917 26.697432849487353 27.361375512317736 23.619477952595737 80-81 22.120775210532674 26.96842995214505 27.538952398101397 23.37184243922088 82-83 22.691813839731978 26.461150724189196 28.120678335871773 22.726357100207053 84-85 22.824192394709073 26.652002746415636 27.374108807959622 23.14969605091567 86-87 22.034857748436348 27.07170992682454 27.668579301150164 23.22485302358895 88-89 22.535889099071195 26.288466160185592 27.670177047994578 23.50546769274863 90-91 22.516754178766824 26.33575126099082 27.653940998260463 23.493553561981898 92-93 22.58562832111288 26.431512025856748 27.644616042730057 23.338243610300317 94-95 21.96024058945395 26.44586329417133 27.96745497577387 23.62644114060085 96-97 22.11920327357078 26.375501320051924 27.715646406407007 23.78964899997029 98-99 21.846764612030633 26.597368369530162 27.535017248006167 24.02084977043304 100 22.319574199214824 26.258018499890685 27.116994745844185 24.305412555050303 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1319.0 1 925.5 2 1155.0 3 1638.5 4 1173.5 5 906.5 6 1376.0 7 2554.5 8 3957.0 9 4597.0 10 4095.5 11 3499.5 12 3698.0 13 4183.5 14 4680.0 15 5355.5 16 6009.0 17 6570.5 18 7274.5 19 7893.5 20 8546.5 21 9362.5 22 10522.5 23 12355.0 24 14490.5 25 17158.0 26 21030.0 27 25708.0 28 31230.5 29 40179.5 30 52494.0 31 64346.5 32 79783.0 33 96877.5 34 110741.0 35 124779.5 36 139107.0 37 159652.5 38 171404.0 39 170822.5 40 168591.5 41 164502.0 42 166602.0 43 175269.0 44 187520.0 45 207774.0 46 226599.5 47 239843.0 48 250616.5 49 252158.0 50 246593.0 51 248038.5 52 246927.0 53 236343.5 54 223520.0 55 199882.5 56 177239.0 57 151700.5 58 126296.5 59 112989.0 60 94180.0 61 71728.0 62 55740.0 63 44922.5 64 33810.5 65 22045.0 66 15040.0 67 10939.0 68 8319.0 69 6240.0 70 4790.5 71 3665.0 72 2833.0 73 2241.0 74 1745.0 75 1287.0 76 879.0 77 563.0 78 389.0 79 279.5 80 205.0 81 148.0 82 113.5 83 91.0 84 72.5 85 60.0 86 41.0 87 31.5 88 29.5 89 25.5 90 20.0 91 14.5 92 15.0 93 13.5 94 10.0 95 6.0 96 2.5 97 3.5 98 3.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05573160783971686 2 0.12950816941928042 3 0.016482545756663025 4 0.015950296883270782 5 0.018766065116700717 6 0.05152512480806848 7 0.05671025899401873 8 0.048966896352086405 9 0.04036506778532789 10-11 0.03949801720125343 12-13 0.04073420813300316 14-15 0.018766065116700717 16-17 0.017881845214129728 18-19 0.018036369080598448 20-21 0.02131570891343453 22-23 0.02564237717455857 24-25 0.025050035686428496 26-27 0.019581607745285605 28-29 0.018688803183466357 30-31 0.016877440082083077 32-33 0.02352196634023786 34-35 0.02335885781452088 36-37 0.023461873725500026 38-39 0.021375801528172363 40-41 0.02494701977544935 42-43 0.03187483978879678 44-45 0.0410175018881958 46-47 0.0473186417764201 48-49 0.04024488255585222 50-51 0.028192020971292385 52-53 0.03956669447523953 54-55 0.03647621714586521 56-57 0.03870822855041332 58-59 0.04338686784071611 60-61 0.023590643614223958 62-63 0.02614887207020603 64-65 0.02564237717455857 66-67 0.0326817977581334 68-69 0.039300570038543405 70-71 0.04056251494803791 72-73 0.044811921275927605 74-75 0.027196200498493993 76-77 0.020740536743800976 78-79 0.02016536457416742 80-81 0.02462938738326366 82-83 0.020774875380794023 84-85 0.0129199121686343 86-87 0.015014569025210226 88-89 0.01874031113895593 90-91 0.021350047550427576 92-93 0.020294134462891352 94-95 0.023942614643402697 96-97 0.02517022091590416 98-99 0.02817485165279586 100 0.029428211903042113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5824343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.94007191203745 #Duplication Level Percentage of deduplicated Percentage of total 1 80.20129444266205 56.09284300758273 2 12.967270890832818 18.138637172154347 3 3.5566620343293116 7.462595953434163 4 1.38455513758879 3.8734354355656344 5 0.6531761384214534 2.2841593046211686 6 0.3551023892792141 1.4901531985394731 7 0.19869898908154274 0.9727915109648569 8 0.13665738296938507 0.7646261753751706 9 0.10306336945806834 0.6487433524254764 >10 0.41594189108427754 4.88972948814845 >50 0.0180973707072202 0.8659727859929691 >100 0.00865447827421635 1.0943344526665657 >500 5.123709603378564E-4 0.25677931400807863 >1k 2.27719528268766E-4 0.3037026454794688 >5k 2.846494103189433E-5 0.18542683971824528 >10k+ 5.692988206378866E-5 0.6760693633231577 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18932 0.3250495377761921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15027 0.2580033490472659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9314 0.1599150324766244 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.433863699304797E-5 0.0 0.0 0.0 1.7169318496523986E-5 7 3.433863699304797E-5 0.0 0.0 0.0 1.7169318496523986E-5 8 3.433863699304797E-5 0.0 0.0 3.433863699304797E-5 1.7169318496523986E-5 9 3.433863699304797E-5 0.0 0.0 5.150795548957196E-5 1.7169318496523986E-5 10-11 3.433863699304797E-5 8.584659248261993E-6 0.0 5.150795548957196E-5 1.7169318496523986E-5 12-13 3.433863699304797E-5 1.7169318496523986E-5 0.0 6.867727398609594E-5 1.7169318496523986E-5 14-15 5.150795548957196E-5 1.7169318496523986E-5 0.0 6.867727398609594E-5 1.7169318496523986E-5 16-17 5.150795548957196E-5 1.7169318496523986E-5 0.0 7.726193323435793E-5 1.7169318496523986E-5 18-19 5.150795548957196E-5 1.7169318496523986E-5 0.0 1.2876988872392988E-4 1.7169318496523986E-5 20-21 5.150795548957196E-5 1.7169318496523986E-5 0.0 2.146164812065498E-4 1.7169318496523986E-5 22-23 5.150795548957196E-5 1.7169318496523986E-5 0.0 4.1206364391657566E-4 1.7169318496523986E-5 24-25 5.150795548957196E-5 1.7169318496523986E-5 0.0 8.498812655779373E-4 1.7169318496523986E-5 26-27 5.150795548957196E-5 1.7169318496523986E-5 0.0 0.0013563761612253949 1.7169318496523986E-5 28-29 5.150795548957196E-5 1.7169318496523986E-5 0.0 0.003588387565773513 1.7169318496523986E-5 30-31 5.150795548957196E-5 1.7169318496523986E-5 0.0 0.013434991723530017 1.7169318496523986E-5 32-33 6.0092614737833954E-5 1.7169318496523986E-5 0.0 0.036725172264064805 1.7169318496523986E-5 34-35 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.07270347917353082 3.433863699304797E-5 36-37 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.1294652461230391 3.433863699304797E-5 38-39 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.2154577778128795 3.433863699304797E-5 40-41 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.3102925085284297 4.292329624130996E-5 42-43 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.401392912470986 5.150795548957196E-5 44-45 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.506606496217685 6.867727398609594E-5 46-47 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.6352561997121393 6.867727398609594E-5 48-49 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.7746453119261691 6.867727398609594E-5 50-51 6.867727398609594E-5 1.7169318496523986E-5 0.0 0.9189620185487015 6.867727398609594E-5 52-53 6.867727398609594E-5 1.7169318496523986E-5 0.0 1.061544624003085 6.867727398609594E-5 54-55 6.867727398609594E-5 1.7169318496523986E-5 0.0 1.2195710314450916 6.867727398609594E-5 56-57 7.726193323435793E-5 1.7169318496523986E-5 0.0 1.4024671967293134 6.867727398609594E-5 58-59 8.584659248261992E-5 1.7169318496523986E-5 0.0 1.6107911227068872 6.867727398609594E-5 60-61 1.2018522947566791E-4 1.7169318496523986E-5 0.0 1.8169517145538991 6.867727398609594E-5 62-63 1.5452386646871587E-4 1.7169318496523986E-5 0.0 2.0274990672767728 6.867727398609594E-5 64-65 1.5452386646871587E-4 1.7169318496523986E-5 0.0 2.243446170666803 6.867727398609594E-5 66-67 1.7169318496523985E-4 1.7169318496523986E-5 0.0 2.4891047797150683 6.867727398609594E-5 68-69 1.7169318496523985E-4 1.7169318496523986E-5 0.0 2.758826188636212 6.867727398609594E-5 70-71 1.7169318496523985E-4 1.7169318496523986E-5 0.0 3.041287231881776 6.867727398609594E-5 72-73 1.7169318496523985E-4 1.7169318496523986E-5 0.0 3.3187605881041002 8.584659248261992E-5 74-75 1.7169318496523985E-4 1.7169318496523986E-5 0.0 3.595212369875881 8.584659248261992E-5 76-77 1.7169318496523985E-4 1.7169318496523986E-5 0.0 3.900937839684236 8.584659248261992E-5 78-79 1.7169318496523985E-4 1.7169318496523986E-5 0.0 4.237224696416403 8.584659248261992E-5 80-81 1.7169318496523985E-4 1.7169318496523986E-5 0.0 4.590337485275164 8.584659248261992E-5 82-83 1.7169318496523985E-4 1.7169318496523986E-5 0.0 4.952034933382186 9.443125173088193E-5 84-85 1.7169318496523985E-4 1.7169318496523986E-5 0.0 5.308864879695443 1.0301591097914392E-4 86-87 1.8027784421350185E-4 1.7169318496523986E-5 0.0 5.688736051431038 1.0301591097914392E-4 88 1.8886250346176385E-4 1.7169318496523986E-5 0.0 5.994478690557888 1.0301591097914392E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9585 0.0 47.425316 1 GTATCAA 16935 0.0 40.37436 1 TACCTGG 4175 0.0 33.475643 2 CAACGCA 19875 0.0 33.206158 5 ATCAACG 19950 0.0 33.081326 3 TATCAAC 21685 0.0 31.270407 2 GTACTAG 3690 0.0 30.351196 1 AACGCAG 22115 0.0 30.225346 6 TCAACGC 22200 0.0 30.046097 4 GTACATG 22445 0.0 27.968004 1 ACCTGGG 5070 0.0 27.5364 3 ACGCAGA 24015 0.0 27.344656 7 TACATGG 22640 0.0 27.24929 2 TCTAACG 1695 0.0 27.207293 2 GTACCTG 5340 0.0 27.141552 1 CGCAGAG 24430 0.0 26.782328 8 CTAACGC 1785 0.0 26.334219 3 CTGTGCG 5045 0.0 26.273203 9 ACATGGG 22560 0.0 26.191168 3 GTGGTAT 3750 0.0 26.10101 1 >>END_MODULE