FastQCFastQC Report
Mon 6 Jun 2016
ERR1211233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5824343
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172360.2959303736060874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147410.2530929239572601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93080.15981201656564525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102500.047.932411
GTATCAA171750.038.1321371
TACCTGG41200.031.8274652
CAACGCA200700.031.5110035
ATCAACG201950.031.1646063
GTACTAG38500.030.89561
TATCAAC215750.030.214872
GTGGTAT38600.029.110351
AACGCAG221500.028.9762066
TCAACGC227150.028.1210584
GTACCTG50750.026.865741
GTACATG226700.026.8153721
TACATGG226900.026.2651462
ACGCAGA246100.025.7361077
TAGTACT55900.025.5615224
CGCAGAG249750.025.2845138
ACCTGGG49350.025.2396683
ACATGGG226300.025.1111533
GGGCACC44100.024.8371694
TCTAACG17850.024.487242
Produced by FastQC (version 0.11.5)