##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211233_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5824343 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12533671179737 33.0 31.0 34.0 30.0 34.0 2 32.26563991852815 34.0 31.0 34.0 30.0 34.0 3 32.34360562212768 34.0 31.0 34.0 30.0 34.0 4 35.965736221235595 37.0 35.0 37.0 35.0 37.0 5 35.84720525559707 37.0 35.0 37.0 35.0 37.0 6 35.874690930805414 37.0 35.0 37.0 35.0 37.0 7 35.811392632611096 37.0 35.0 37.0 35.0 37.0 8 35.79692370452771 37.0 35.0 37.0 33.0 37.0 9 37.399923218807686 39.0 37.0 39.0 34.0 39.0 10-11 37.37672120271763 39.0 37.0 39.0 34.0 39.0 12-13 37.274921652107366 39.0 37.0 39.0 34.0 39.0 14-15 38.49153071857204 40.0 38.0 41.0 34.0 41.0 16-17 38.4418221076609 40.0 38.0 41.0 34.0 41.0 18-19 38.462740346851135 40.0 38.0 41.0 34.0 41.0 20-21 38.42540866841118 40.0 38.0 41.0 34.0 41.0 22-23 38.29474071152747 40.0 38.0 41.0 34.0 41.0 24-25 38.234354501443335 40.0 38.0 41.0 34.0 41.0 26-27 38.09630253575382 40.0 37.0 41.0 33.0 41.0 28-29 38.07150900968573 40.0 37.0 41.0 33.0 41.0 30-31 38.086241916041004 40.0 37.0 41.0 33.0 41.0 32-33 37.95641834624094 40.0 37.0 41.0 33.0 41.0 34-35 37.81667382913403 40.0 37.0 41.0 33.0 41.0 36-37 37.67355648525508 40.0 37.0 41.0 33.0 41.0 38-39 37.583553715844005 40.0 37.0 41.0 33.0 41.0 40-41 37.48461045305882 40.0 36.0 41.0 32.0 41.0 42-43 37.34066486125559 40.0 36.0 41.0 32.0 41.0 44-45 37.19573023086038 39.0 36.0 41.0 31.0 41.0 46-47 36.9579969792301 39.0 36.0 41.0 31.0 41.0 48-49 36.721569625964676 39.0 35.0 41.0 30.5 41.0 50-51 36.521263428338614 39.0 35.0 40.5 30.0 41.0 52-53 36.31811673866048 39.0 35.0 40.0 30.0 41.0 54-55 36.07937478956855 38.0 35.0 40.0 29.0 41.0 56-57 35.82162880860554 38.0 35.0 40.0 28.5 41.0 58-59 35.54658542603003 38.0 34.0 40.0 28.0 41.0 60-61 35.28060426729675 37.0 34.0 40.0 28.0 41.0 62-63 34.97243560346635 37.0 34.0 40.0 27.5 41.0 64-65 34.601037318715605 36.0 33.5 39.0 26.5 41.0 66-67 34.297009808659965 36.0 33.0 39.0 26.0 40.5 68-69 33.97046121768584 35.0 33.0 38.5 26.0 40.0 70-71 33.65087143734495 35.0 33.0 38.0 26.0 40.0 72-73 33.31298259735047 35.0 32.0 37.0 26.0 39.5 74-75 32.979348486172604 35.0 32.0 37.0 25.0 39.0 76-77 31.989846065041156 34.0 30.5 36.0 24.5 38.0 78-79 32.49109839513229 35.0 32.0 36.0 25.0 38.0 80-81 32.4225021603295 35.0 32.0 35.5 25.0 37.0 82-83 32.17439615077615 34.5 32.0 35.0 25.0 37.0 84-85 31.924212396144938 34.0 31.0 35.0 25.0 36.5 86-87 31.689800892564193 34.0 31.0 35.0 24.0 36.0 88-89 31.47725743487291 34.0 31.0 35.0 24.0 36.0 90-91 31.264725738164802 34.0 31.0 35.0 23.5 35.0 92-93 31.080907065397763 34.0 31.0 35.0 23.0 35.0 94-95 30.894068137814 34.0 30.0 35.0 21.5 35.0 96-97 30.723702913787875 34.0 30.0 35.0 20.0 35.0 98-99 30.54801708965286 34.0 30.0 35.0 20.0 35.0 100 28.751640485459045 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.42401376321138073 1101 2 -0.4157176028288809 1101 3 -0.4709392802264851 1101 4 -0.3015238555413404 1101 5 -0.2775522312185643 1101 6 -0.34989781360867056 1101 7 -0.3202752458248881 1101 8 -0.42837356522667136 1101 9 -0.47563894393486095 1101 10-11 -0.4695541345392584 1101 12-13 -0.5494873138674663 1101 14-15 -0.7311366944587903 1101 16-17 -0.6850087392769098 1101 18-19 -0.7823881915759188 1101 20-21 -0.8419661723279788 1101 22-23 -0.7700341638151897 1101 24-25 -0.7481389182522449 1101 26-27 -0.8325537312340501 1101 28-29 -0.9260112692360991 1101 30-31 -0.7525985706872262 1101 32-33 -0.7834059937458235 1101 34-35 -0.7509494452764258 1101 36-37 -0.7487502339349561 1101 38-39 -0.805414360953236 1101 40-41 -0.8405060038046699 1101 42-43 -0.7798078429352486 1101 44-45 -0.9018248222303384 1101 46-47 -0.8482557485890183 1101 48-49 -0.7781741856587345 1101 50-51 -0.8713933817220365 1101 52-53 -0.9823410957624645 1101 54-55 -0.8935125577283003 1101 56-57 -0.9820615367002361 1101 58-59 -0.967577754326534 1101 60-61 -1.0284822429502043 1101 62-63 -1.1237869733905725 1101 64-65 -0.9496273380726663 1101 66-67 -0.987114866103532 1101 68-69 -1.0864273053367484 1101 70-71 -1.1264122724547292 1101 72-73 -1.223417701214487 1101 74-75 -1.1641887300040388 1101 76-77 -1.105763877134411 1101 78-79 -1.1251710358999922 1101 80-81 -0.9521559517765112 1101 82-83 -0.9545377051356283 1101 84-85 -0.9157332365456057 1101 86-87 -1.062543030546319 1101 88-89 -1.0047857681591452 1101 90-91 -1.1219653429850531 1101 92-93 -1.2106728088507666 1101 94-95 -1.151358972999482 1101 96-97 -1.1863390804641156 1101 98-99 -1.266981268190964 1101 100 -1.1121493661308612 1102 1 -0.4007270972640775 1102 2 -0.3811754698507208 1102 3 -0.3958030171497633 1102 4 -0.29777725842748737 1102 5 -0.3445143738920322 1102 6 -0.42900711235241573 1102 7 -0.37176795260306505 1102 8 -0.40549231984683587 1102 9 -0.5510062623773635 1102 10-11 -0.5618297775780476 1102 12-13 -0.5360253325140221 1102 14-15 -0.6557100553426878 1102 16-17 -0.6988749064688733 1102 18-19 -0.6488234807736006 1102 20-21 -0.7239021951157056 1102 22-23 -0.7601813488747595 1102 24-25 -0.7749507353598943 1102 26-27 -0.8726302414820353 1102 28-29 -0.8878903549139423 1102 30-31 -0.828913140972027 1102 32-33 -0.6632193027281588 1102 34-35 -0.8002103526304225 1102 36-37 -0.862729888822841 1102 38-39 -0.8585419025488363 1102 40-41 -0.7912797225452195 1102 42-43 -0.7891553853146362 1102 44-45 -0.8922888494759746 1102 46-47 -0.9577361585405058 1102 48-49 -0.8835121336573124 1102 50-51 -0.9790446749395585 1102 52-53 -1.070675322932189 1102 54-55 -0.8730001130025542 1102 56-57 -0.9676116648167863 1102 58-59 -0.9425029861200542 1102 60-61 -0.9269209013474153 1102 62-63 -1.1056634607692288 1102 64-65 -0.8862915943336844 1102 66-67 -0.9824134477220738 1102 68-69 -1.0695414205970835 1102 70-71 -1.1390259413337063 1102 72-73 -1.1258975737904606 1102 74-75 -1.1401960138311793 1102 76-77 -0.9424550290130504 1102 78-79 -0.7713791564438957 1102 80-81 -0.8586999615426762 1102 82-83 -0.8763446826426247 1102 84-85 -1.0259832584217818 1102 86-87 -0.9412938284228005 1102 88-89 -0.9720112838866406 1102 90-91 -0.9364021160048246 1102 92-93 -1.1439965327718156 1102 94-95 -1.18875928876799 1102 96-97 -1.3467070189552892 1102 98-99 -1.1954697935327019 1102 100 -1.0926626590617161 1103 1 -0.3316725901143336 1103 2 -0.36339746159652364 1103 3 -0.3899904018133391 1103 4 -0.2353343864608064 1103 5 -0.20484388184058844 1103 6 -0.26631548299412344 1103 7 -0.36906572132940596 1103 8 -0.31389177110343525 1103 9 -0.4410265809691083 1103 10-11 -0.4641490513258901 1103 12-13 -0.5018893126637138 1103 14-15 -0.6306499350444668 1103 16-17 -0.7216063768193948 1103 18-19 -0.6420604121057636 1103 20-21 -0.6351884159257324 1103 22-23 -0.7113508857585771 1103 24-25 -0.7628445423013162 1103 26-27 -0.7509457102736903 1103 28-29 -0.7581507251743105 1103 30-31 -0.6689693143615258 1103 32-33 -0.6557886381546396 1103 34-35 -0.7629555677565847 1103 36-37 -0.7872088942542348 1103 38-39 -0.7395865264506014 1103 40-41 -0.8274227205929847 1103 42-43 -0.7848054571551799 1103 44-45 -0.7046917285455194 1103 46-47 -0.7893001662949928 1103 48-49 -0.7758288354978262 1103 50-51 -0.9357368982984511 1103 52-53 -0.894673889787903 1103 54-55 -0.9306505944625556 1103 56-57 -0.9227078913415809 1103 58-59 -0.8917211210524769 1103 60-61 -0.974988958939278 1103 62-63 -0.8666952408486353 1103 64-65 -0.8636130811789826 1103 66-67 -0.9885874396103489 1103 68-69 -1.0508218114012848 1103 70-71 -1.0798558869255572 1103 72-73 -0.9275323325680702 1103 74-75 -0.9401868471553527 1103 76-77 -0.8606049424010607 1103 78-79 -0.72256849334752 1103 80-81 -0.7093594122021258 1103 82-83 -0.731954714443134 1103 84-85 -0.7900861891913813 1103 86-87 -0.675859424893158 1103 88-89 -0.8195434314187864 1103 90-91 -0.8545918389564555 1103 92-93 -0.8594688863394069 1103 94-95 -0.9437031913309433 1103 96-97 -1.0871633308401734 1103 98-99 -1.0187784501746933 1103 100 -1.1062434821663487 1104 1 -0.43660015742002756 1104 2 -0.39448892216021747 1104 3 -0.4030625145304292 1104 4 -0.28818705972997094 1104 5 -0.30544967672891943 1104 6 -0.36797402474910257 1104 7 -0.37280335801532516 1104 8 -0.45382983791729714 1104 9 -0.582323630304522 1104 10-11 -0.5859648790684915 1104 12-13 -0.6056665441433609 1104 14-15 -0.8223688844335086 1104 16-17 -0.7608929571985925 1104 18-19 -0.7983445784710028 1104 20-21 -0.8118690328352614 1104 22-23 -0.7572744360271884 1104 24-25 -0.7947050018191533 1104 26-27 -0.8796948209934428 1104 28-29 -0.9252652883385792 1104 30-31 -0.8517570326648283 1104 32-33 -0.9287427752991064 1104 34-35 -1.0515359068615098 1104 36-37 -0.9339551890108027 1104 38-39 -0.9508617475022163 1104 40-41 -0.9909890453504957 1104 42-43 -0.8860840771122653 1104 44-45 -0.9955023436328432 1104 46-47 -1.079094996930749 1104 48-49 -1.1145306697883584 1104 50-51 -1.0032525514038682 1104 52-53 -0.9613983630803062 1104 54-55 -1.0095324629931142 1104 56-57 -1.01561070528615 1104 58-59 -1.0851619427255983 1104 60-61 -1.143483918275166 1104 62-63 -1.178882816376074 1104 64-65 -1.1841362609778088 1104 66-67 -1.1161420062280811 1104 68-69 -1.1087900454107427 1104 70-71 -1.2450318668932994 1104 72-73 -1.1505063114370344 1104 74-75 -1.2049605096478544 1104 76-77 -1.0737556650367317 1104 78-79 -0.9416946813959228 1104 80-81 -0.9411161928276037 1104 82-83 -1.0363270067075376 1104 84-85 -1.0357229874988008 1104 86-87 -1.131030954367887 1104 88-89 -1.0165620375620215 1104 90-91 -1.0308170066239697 1104 92-93 -1.1521372378647925 1104 94-95 -1.1815532806408697 1104 96-97 -1.1514277405899556 1104 98-99 -1.30262345722074 1104 100 -1.3817541977205146 1105 1 -0.44379667547967827 1105 2 -0.40271359427152476 1105 3 -0.4633750957742109 1105 4 -0.25431848863438233 1105 5 -0.3109966658904355 1105 6 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68-69 -0.8356745089104365 1105 70-71 -0.8532614696616321 1105 72-73 -0.8973747839194814 1105 74-75 -0.9387016680833113 1105 76-77 -0.906469117662084 1105 78-79 -0.7927622246736412 1105 80-81 -0.7894477817542409 1105 82-83 -0.7945132981362661 1105 84-85 -0.7862785268678572 1105 86-87 -0.8623238734961447 1105 88-89 -0.9085422678685298 1105 90-91 -0.9309681200559758 1105 92-93 -0.8567216843313048 1105 94-95 -0.8152631742283241 1105 96-97 -0.9387452502495641 1105 98-99 -0.8739798697643302 1105 100 -0.9400087899551792 1106 1 -0.4783582318562303 1106 2 -0.4439682179258888 1106 3 -0.5380052034251399 1106 4 -0.29676639504589986 1106 5 -0.36857745539345643 1106 6 -0.444683654202386 1106 7 -0.5330473520686212 1106 8 -0.46800991362934496 1106 9 -0.6149150398899579 1106 10-11 -0.6387106974183965 1106 12-13 -0.6784435171756442 1106 14-15 -0.7428686906317878 1106 16-17 -0.825908702396859 1106 18-19 -0.7494454110693525 1106 20-21 -0.7669443499319257 1106 22-23 -0.7800244157345233 1106 24-25 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1107 46-47 -0.8720287346801001 1107 48-49 -0.8501224972301387 1107 50-51 -0.8265973921352412 1107 52-53 -0.8560546464889924 1107 54-55 -0.8212779309968283 1107 56-57 -0.8547669174138903 1107 58-59 -0.9538578039602683 1107 60-61 -0.9436287528461236 1107 62-63 -0.9145499453921389 1107 64-65 -0.8255882749984877 1107 66-67 -0.8258697702524316 1107 68-69 -0.9243710553213802 1107 70-71 -0.9620644799028994 1107 72-73 -0.8340329608345414 1107 74-75 -0.8597511583235544 1107 76-77 -0.902136971492471 1107 78-79 -0.7485979685515574 1107 80-81 -0.6272354652058674 1107 82-83 -0.70392456211178 1107 84-85 -0.7753282355210622 1107 86-87 -0.7435018797826665 1107 88-89 -0.600871792666311 1107 90-91 -0.7356793008291973 1107 92-93 -0.7743145362051358 1107 94-95 -0.891225284019832 1107 96-97 -0.9189888854682771 1107 98-99 -1.0313928598128044 1107 100 -1.0336880034797993 1108 1 -0.4306201602420714 1108 2 -0.422330552796673 1108 3 -0.4650794499582531 1108 4 -0.284020726513873 1108 5 -0.3515078434949146 1108 6 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1.2251838383429963 2310 78-79 1.0430191438233365 2310 80-81 1.0220770627246978 2310 82-83 1.0449492658797226 2310 84-85 1.0799063478579178 2310 86-87 1.057902968935693 2310 88-89 1.07726875525589 2310 90-91 1.1622710999787067 2310 92-93 1.1736167452374104 2310 94-95 1.2447929154444424 2310 96-97 1.3026584986080074 2310 98-99 1.272132037341997 2310 100 1.373624140558416 2311 1 0.3677814102515242 2311 2 0.32671669052371044 2311 3 0.41975977571799206 2311 4 0.28607177932225625 2311 5 0.418679668734633 2311 6 0.4456959098683342 2311 7 0.4924023924183416 2311 8 0.533018814408976 2311 9 0.6235378696135783 2311 10-11 0.6308256363539684 2311 12-13 0.6780031134293125 2311 14-15 0.8501633725165618 2311 16-17 0.6620647317757289 2311 18-19 0.7731819894588199 2311 20-21 0.6751299521433936 2311 22-23 0.9222793740115023 2311 24-25 0.9091832026747468 2311 26-27 0.28264665918494103 2311 28-29 0.6781216472682772 2311 30-31 0.9148823333199019 2311 32-33 0.9683632990672137 2311 34-35 1.032700130634673 2311 36-37 1.0614908602206015 2311 38-39 1.1028516290574615 2311 40-41 1.1142966816127426 2311 42-43 1.1291191953712953 2311 44-45 1.1647130663911867 2311 46-47 0.7914233764346363 2311 48-49 1.0644600412652139 2311 50-51 1.0946677172125518 2311 52-53 1.0326889251129074 2311 54-55 1.0952887035132122 2311 56-57 1.2104229089624639 2311 58-59 1.2134278442788613 2311 60-61 1.2273692267015832 2311 62-63 1.198935355177106 2311 64-65 1.2214666573961992 2311 66-67 1.2785921345601068 2311 68-69 1.306082613892201 2311 70-71 1.2600385350357328 2311 72-73 1.3479362362135632 2311 74-75 1.32879412395328 2311 76-77 1.2869391124378318 2311 78-79 0.9913226020025405 2311 80-81 0.9717827157485104 2311 82-83 1.07322043571682 2311 84-85 1.1435140422811862 2311 86-87 1.1654199726172436 2311 88-89 1.1673451831294024 2311 90-91 1.2025384810917963 2311 92-93 1.2834694344104634 2311 94-95 1.3412601270670308 2311 96-97 1.3593414636889385 2311 98-99 1.3883627122856197 2311 100 1.4929516215880234 2312 1 0.2414120794368131 2312 2 0.34446877547708965 2312 3 0.4218230601854316 2312 4 0.1647411077858436 2312 5 0.32529389515955387 2312 6 0.3986946248225891 2312 7 0.36865017552143087 2312 8 0.44519859694077724 2312 9 0.6592786211091735 2312 10-11 0.6295981843098204 2312 12-13 0.7297206997119616 2312 14-15 0.9077910572690868 2312 16-17 0.8765978135449188 2312 18-19 0.7849260600710721 2312 20-21 0.8739452062890862 2312 22-23 0.9093182362056069 2312 24-25 0.9021202651731741 2312 26-27 0.9617071861730082 2312 28-29 0.9135844576162953 2312 30-31 0.9540027669684505 2312 32-33 0.867311433241845 2312 34-35 0.9474875853141782 2312 36-37 1.042417914398854 2312 38-39 0.7995798133706344 2312 40-41 0.9547422704352968 2312 42-43 1.0702394507557145 2312 44-45 0.8155824308058826 2312 46-47 0.938213139511781 2312 48-49 1.04593416980628 2312 50-51 0.8638767736619215 2312 52-53 1.01767899995928 2312 54-55 0.955984235400031 2312 56-57 1.0007023335974523 2312 58-59 1.1428306546233742 2312 60-61 1.2022440294881136 2312 62-63 1.2155609525198443 2312 64-65 1.2094202308199655 2312 66-67 1.153021823134786 2312 68-69 1.1888458214920021 2312 70-71 1.1338253936884186 2312 72-73 1.0933061500712853 2312 74-75 1.1361289712742675 2312 76-77 1.1299193724051797 2312 78-79 1.0678024859659274 2312 80-81 0.9932631374870482 2312 82-83 1.025935919500398 2312 84-85 1.082041012977097 2312 86-87 1.077282112509664 2312 88-89 1.0445664774287344 2312 90-91 1.1159480733687523 2312 92-93 1.1572965494371488 2312 94-95 1.2562931445312984 2312 96-97 0.8375811334932415 2312 98-99 0.624020356465504 2312 100 0.8914602254640762 2313 1 0.038056778299051075 2313 2 0.23931357772805484 2313 3 0.28475601339220447 2313 4 0.16084540831527505 2313 5 0.3012336660559427 2313 6 0.2871280028956278 2313 7 0.24412480924670632 2313 8 0.38558674779312696 2313 9 0.6018413449226543 2313 10-11 0.41514249765420885 2313 12-13 0.46457046275935454 2313 14-15 0.5248957156759175 2313 16-17 0.750307017158633 2313 18-19 0.7472412932509158 2313 20-21 0.7383237652337655 2313 22-23 0.6544444627827843 2313 24-25 0.5716675856016522 2313 26-27 0.7310949856140567 2313 28-29 0.5609419455048439 2313 30-31 0.8333155410451241 2313 32-33 0.9152360869861909 2313 34-35 0.9810758908530275 2313 36-37 1.0362455421553136 2313 38-39 1.0025382357063322 2313 40-41 0.9972793863141263 2313 42-43 1.0753335028521818 2313 44-45 0.939693481346346 2313 46-47 0.9236609095846404 2313 48-49 0.9840570713393859 2313 50-51 0.9761239316790622 2313 52-53 1.044072922112882 2313 54-55 0.9730476853140928 2313 56-57 0.743025751143044 2313 58-59 0.8151091504071246 2313 60-61 0.6635927953757701 2313 62-63 0.9712043218644979 2313 64-65 1.0806999783556037 2313 66-67 0.9865252976752217 2313 68-69 1.0795353855592182 2313 70-71 1.04516530747712 2313 72-73 1.058281108136434 2313 74-75 1.152737412094396 2313 76-77 1.1047274131199138 2313 78-79 0.9477851055044013 2313 80-81 0.9345542045876343 2313 82-83 0.9823809631181604 2313 84-85 0.9903845871852361 2313 86-87 1.0376573630497177 2313 88-89 1.0169685448065913 2313 90-91 0.8837954631828602 2313 92-93 1.020308087384052 2313 94-95 1.1213702584478753 2313 96-97 1.2276088808467946 2313 98-99 1.2379723787358081 2313 100 1.3287345368151797 2314 1 -0.3331224432393185 2314 2 0.05121129971112737 2314 3 0.2765324681826655 2314 4 0.24376947458522125 2314 5 0.35276679415557055 2314 6 0.3795724593387746 2314 7 0.4366501647026624 2314 8 0.46135371637993217 2314 9 0.49710129074739484 2314 10-11 0.6224889039740091 2314 12-13 0.4367093508284583 2314 14-15 0.7537609324205476 2314 16-17 0.8360889800240585 2314 18-19 0.8598676387451221 2314 20-21 0.8839877240318614 2314 22-23 0.8726608826916689 2314 24-25 0.8277081297655187 2314 26-27 0.6945689115495028 2314 28-29 0.768791528760481 2314 30-31 0.8420332921112603 2314 32-33 0.9037605255475469 2314 34-35 0.9097618788488901 2314 36-37 0.8587977936614521 2314 38-39 0.6101101221381597 2314 40-41 0.6703189917006611 2314 42-43 0.8647268847612537 2314 44-45 0.8966088880933967 2314 46-47 0.8726771238462092 2314 48-49 0.88989378336003 2314 50-51 0.8046305169542833 2314 52-53 0.8268312686541037 2314 54-55 0.7835939917614709 2314 56-57 0.7947441697580828 2314 58-59 0.9765158231797173 2314 60-61 1.1127606795660085 2314 62-63 0.9768131433195677 2314 64-65 0.97518722709831 2314 66-67 0.7293664602734324 2314 68-69 0.7251150518451297 2314 70-71 0.9049158980592011 2314 72-73 1.0859264006338591 2314 74-75 1.1219083820843778 2314 76-77 0.8973355540305867 2314 78-79 0.8384710585381967 2314 80-81 0.9023983666284678 2314 82-83 0.9213279374162724 2314 84-85 0.9670429603546289 2314 86-87 0.9858770796206215 2314 88-89 1.0085312028485056 2314 90-91 1.0821495782588322 2314 92-93 0.9556117974032219 2314 94-95 1.0271057419001721 2314 96-97 1.0019844245612681 2314 98-99 1.0940449421068408 2314 100 1.152580562576766 2315 1 0.38378797719131796 2315 2 0.42959439241104036 2315 3 0.4468353586514411 2315 4 0.34333460561702367 2315 5 0.39450992156874776 2315 6 0.41587913398310405 2315 7 0.40158111946723096 2315 8 0.2750725259353999 2315 9 0.4823620459270117 2315 10-11 0.5778937744217174 2315 12-13 0.42849729556358795 2315 14-15 0.6661914415374994 2315 16-17 0.8240299348929128 2315 18-19 0.7892443463820413 2315 20-21 0.570451654438493 2315 22-23 0.8371805910703074 2315 24-25 0.8853532797182098 2315 26-27 0.8161962267988301 2315 28-29 0.7816398875063229 2315 30-31 0.8398070908343058 2315 32-33 0.8148590412069652 2315 34-35 0.8706603541311679 2315 36-37 0.8152935638382957 2315 38-39 0.8981532541463153 2315 40-41 0.8451010480213981 2315 42-43 0.9223898769126393 2315 44-45 0.9522897871316474 2315 46-47 0.8608751408039552 2315 48-49 0.7605003456066513 2315 50-51 0.8698216745235499 2315 52-53 0.9073494655785339 2315 54-55 0.728728354923696 2315 56-57 0.7489158306109616 2315 58-59 0.7728237019687825 2315 60-61 0.865468884989518 2315 62-63 0.9106890383624702 2315 64-65 1.0567109826416115 2315 66-67 1.0451745179733152 2315 68-69 1.064820881241772 2315 70-71 0.9799504092709412 2315 72-73 1.0176371570844296 2315 74-75 0.3598989529304575 2315 76-77 0.42121957745780136 2315 78-79 0.7656094256853478 2315 80-81 0.756168044373922 2315 82-83 0.6371992534503335 2315 84-85 0.7248413250967509 2315 86-87 0.760737148714739 2315 88-89 0.7697517461116838 2315 90-91 0.8730570349291789 2315 92-93 0.8998629309938693 2315 94-95 1.0141314897408584 2315 96-97 1.1102555505392537 2315 98-99 1.1563170874528126 2315 100 1.137051250532842 2316 1 0.3169551254272136 2316 2 0.1326700002665504 2316 3 0.22517481850645993 2316 4 0.04247326460522771 2316 5 0.32200648361529005 2316 6 0.3570375836406541 2316 7 0.4402107389068064 2316 8 0.44179277959397467 2316 9 0.6035089122299055 2316 10-11 0.6427500928871339 2316 12-13 0.4153421053749753 2316 14-15 0.5393373226961842 2316 16-17 0.8340501373672851 2316 18-19 0.7734502198732045 2316 20-21 0.7666243834532906 2316 22-23 0.8211621545281886 2316 24-25 0.5271886205118435 2316 26-27 0.788325221929135 2316 28-29 0.9354559652929879 2316 30-31 0.6295662300928058 2316 32-33 0.7381470022379872 2316 34-35 0.8855828742399652 2316 36-37 0.9095525770692205 2316 38-39 0.7631362781917161 2316 40-41 0.7798536813075927 2316 42-43 0.6936536829974358 2316 44-45 0.5803925245169665 2316 46-47 0.7220057894587342 2316 48-49 0.785935759245298 2316 50-51 0.8544843161588531 2316 52-53 0.8559695916156116 2316 54-55 0.7442839568697721 2316 56-57 0.8914089651121699 2316 58-59 0.761103643983013 2316 60-61 0.8790135473385376 2316 62-63 0.9041428014717994 2316 64-65 0.9492258552621777 2316 66-67 0.9445676467768109 2316 68-69 0.963238887375077 2316 70-71 0.9672890104256382 2316 72-73 0.9551492230788554 2316 74-75 1.0164963204947526 2316 76-77 0.8304210884563439 2316 78-79 0.5628949798813068 2316 80-81 0.554248511933217 2316 82-83 0.537464494191525 2316 84-85 0.6711944121059652 2316 86-87 0.6945403228013305 2316 88-89 0.6952805176872836 2316 90-91 0.5340070520044513 2316 92-93 0.4025508152545356 2316 94-95 0.43501758187646544 2316 96-97 0.5920406123415276 2316 98-99 0.7644334826814401 2316 100 0.35813648882128746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 1.0 11 1.0 12 22.0 13 116.0 14 494.0 15 1466.0 16 3432.0 17 6975.0 18 11663.0 19 17833.0 20 25092.0 21 35114.0 22 43957.0 23 46206.0 24 46762.0 25 50443.0 26 59144.0 27 73217.0 28 90345.0 29 111928.0 30 138096.0 31 172215.0 32 218622.0 33 287935.0 34 407940.0 35 566827.0 36 877308.0 37 1293889.0 38 1071387.0 39 165889.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.239574566029184 20.23516700962929 13.789794256778809 23.73546416756272 2 15.436985768180206 19.85981938220328 40.96709276909001 23.736102080526507 3 19.069841868859715 25.379102844732877 29.362779630251858 26.18827565615555 4 12.615346248712505 16.280062332102048 38.2342146081289 32.870376811056545 5 13.249786284908014 37.75807846481569 34.48335717865517 14.508778071621126 6 31.163240214389848 37.05720971446908 17.559336735491023 14.220213335650048 7 27.833873226632083 31.932443715544135 22.05558128111211 18.17810177671167 8 26.303447444630233 36.063174850794326 19.360982002605272 18.27239570197016 9 26.287383815471582 15.858430450692085 20.833987269283668 37.020198464552664 10-11 25.90286076314488 26.230796041426295 27.10230550519202 20.7640376902368 12-13 25.748683220466518 23.633122537007395 27.909364876807985 22.708829365718106 14-15 23.01929945816127 25.10722898899436 25.670640997696818 26.202830555147543 16-17 22.0963564100867 27.85744509942741 26.736173439512168 23.310025050973724 18-19 21.72146283280363 27.383054191691663 28.9901367416033 21.905346233901405 20-21 22.8783849850863 26.589773867179 28.708004379893527 21.823836767841176 22-23 22.92583043272005 26.357796922330984 28.43186776602958 22.28450487891939 24-25 22.35008579991455 26.32661931510644 29.079908483525035 22.243386401453975 26-27 22.380665447237213 26.610652577898303 28.41359622348301 22.595085751381472 28-29 21.90955011134328 26.969445412605396 28.767884031296305 22.35312044475502 30-31 22.69768185350075 26.622821449914024 28.669686079660206 22.00981061692502 32-33 22.781947411613007 26.749129768884544 28.17523383463385 22.2936889848686 34-35 22.746894145797896 26.695838598881693 28.651432562611905 21.90583469270851 36-37 22.557776112989437 26.566964851555035 28.358049257354406 22.51720977810112 38-39 22.61579412850654 26.663114690754846 28.663337941456795 22.05775323928182 40-41 22.46955238651531 26.082728707789098 29.01724440470267 22.430474500992915 42-43 22.429386407624968 26.296262975077518 28.48431384230897 22.790036774988543 44-45 22.371810105733395 26.902655079997196 28.38076935228851 22.3447654619809 46-47 22.386248913203445 26.687894009366154 27.925704415034275 23.000152662396125 48-49 22.845988777043935 26.693977164673754 27.97207275794135 22.48796130034096 50-51 22.66100944184401 26.719912852706184 27.815143748303804 22.803933957145997 52-53 22.35511534494016 27.05442519417332 27.613766269112006 22.976693191774512 54-55 22.265425754454643 26.957593218019426 27.70261446858811 23.074366558937818 56-57 22.84145224825349 26.680861168194543 27.430493614620488 23.047192968931483 58-59 22.262239766600512 26.980677421379333 27.9344991688895 22.822583643130656 60-61 22.514811817036414 27.31157236198903 27.46616367328255 22.70745214769201 62-63 22.641245897659388 26.941143575761856 27.30294879192084 23.11466173465792 64-65 22.129716921585576 26.94620811090583 27.48885364895611 23.435221318552486 66-67 22.385644803759185 26.713796805371327 27.445530562378273 23.455027828491215 68-69 22.120976005474116 26.81916562508026 27.559650453022442 23.500207916423182 70-71 22.11291391513445 26.485234746453628 27.71172153583511 23.690129802576813 72-73 22.41433892168481 26.695550589837012 27.613530978016186 23.276579510461993 74-75 22.614645292770945 26.89322306827393 27.304505705546124 23.187625933409002 76-77 22.423016870654877 26.778723213505177 27.137350649903812 23.66090926593613 78-79 22.279723391082253 26.70986724121662 27.324545139128283 23.685864228572843 80-81 22.008599709508488 27.084062860104357 27.44339566192584 23.463941768461318 82-83 22.63747731927386 26.53869962233714 27.97571813997778 22.84810491841122 84-85 22.801213263511872 26.738539205170508 27.205812629566744 23.254434901750873 86-87 21.979815978053907 27.11519628832928 27.524379227212275 23.38060850640454 88-89 22.42814626610056 26.41238521202358 27.480887569979256 23.678580951896606 90-91 22.39677389931181 26.402903229519044 27.536748582886556 23.66357428828259 92-93 22.486457547171433 26.538080494505213 27.41868774327721 23.556774215046147 94-95 21.872117326981236 26.516556459694606 27.754298552713554 23.857027660610605 96-97 21.97055975846722 26.45515550200828 27.554872078043495 24.019412661481006 98-99 21.65100816824763 26.723765746940252 27.409688004676596 24.215538080135516 100 21.929540120162805 26.390990434584573 27.18694621527698 24.492523229975642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1202.0 1 842.5 2 946.5 3 1396.0 4 1037.0 5 765.5 6 1196.5 7 2303.0 8 3531.5 9 4107.5 10 3650.0 11 3057.5 12 3242.5 13 3730.5 14 4322.0 15 5025.5 16 5653.5 17 6415.0 18 7244.5 19 7918.5 20 8657.5 21 9491.5 22 10803.0 23 12696.0 24 14861.0 25 17719.5 26 21729.5 27 26220.0 28 31841.5 29 40697.0 30 52719.0 31 64844.0 32 79900.0 33 96587.0 34 111210.5 35 125644.0 36 140016.0 37 161004.0 38 172362.0 39 170573.0 40 167706.5 41 163854.0 42 165832.0 43 174191.0 44 186905.0 45 208497.0 46 229042.0 47 242129.5 48 252348.0 49 253168.0 50 247528.5 51 248219.5 52 246756.0 53 236702.5 54 224815.5 55 202063.0 56 178443.0 57 152865.5 58 127834.5 59 114850.0 60 94578.5 61 70275.0 62 54128.5 63 42891.0 64 31301.5 65 19960.5 66 13476.0 67 9718.0 68 7191.0 69 5216.0 70 3932.5 71 3077.5 72 2397.5 73 1903.0 74 1460.5 75 966.0 76 602.5 77 353.0 78 222.5 79 141.5 80 84.5 81 51.0 82 35.0 83 24.5 84 14.0 85 11.0 86 7.0 87 3.5 88 3.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04331819056673002 2 0.0 3 0.0 4 0.034029589260110536 5 0.0 6 0.0 7 7.554500138470554E-4 8 0.0 9 0.010868178608299682 10-11 3.9489432542005165E-4 12-13 8.584659248261993E-6 14-15 1.4593920722045386E-4 16-17 3.1763239218569374E-4 18-19 0.0 20-21 8.584659248261993E-6 22-23 0.0 24-25 6.266801251231255E-4 26-27 5.923414881300775E-4 28-29 2.2320114045481184E-4 30-31 0.002446627885754668 32-33 0.015280693461906348 34-35 0.02589991695200643 36-37 0.030578556242309218 38-39 0.022199928816005514 40-41 0.0013220375242323469 42-43 6.867727398609594E-5 44-45 0.010971194519278827 46-47 0.01778741396239885 48-49 0.0035883875657735133 50-51 0.01097977917852709 52-53 0.010567715534610514 54-55 0.010584884853107037 56-57 3.777250069235277E-4 58-59 0.008730598455482448 60-61 0.0047559012235371435 62-63 0.001313452864984085 64-65 0.03492239382192979 66-67 0.060264307922799194 68-69 0.059998183486103064 70-71 0.05221189754792944 72-73 0.045498694015788564 74-75 0.035531904628556384 76-77 0.03203794831451376 78-79 0.015220600847168513 80-81 0.001313452864984085 82-83 0.038098717743786724 84-85 0.05183417254100591 86-87 0.05756014025959666 88-89 0.06948423195543257 90-91 0.048520494071176784 92-93 0.03916321549057121 94-95 0.01296283546487561 96-97 0.003536879610283941 98-99 0.02244029927495685 100 0.03277622900986429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5824343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.36294753736047 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73402053664587 53.82489715352183 2 13.837658045984428 18.919661820751347 3 3.885035641742289 7.967774632716111 4 1.4666659026530673 4.010624166316283 5 0.7114562883900467 2.4318624459166984 6 0.390720444956957 1.6026480768219955 7 0.24040663036332005 1.1504434101412884 8 0.15672893164885685 0.8571561385517885 9 0.11392244374628803 0.7009266640639849 >10 0.43423667703663943 5.034870544408669 >50 0.01928073175107055 0.9104364236953651 >100 0.00892770669698236 1.1059048621596252 >500 4.700098693104637E-4 0.22092238740205475 >1k 3.818819183764083E-4 0.4327867435328694 >5k 2.9375532180866076E-5 0.18692306661595007 >10k+ 5.875106436173215E-5 0.6421614633839959 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17236 0.2959303736060874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14741 0.2530929239572601 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9308 0.15981201656564525 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.584659248261992E-5 0.0 0.0 0.0 0.0 7 8.584659248261992E-5 0.0 0.0 0.0 0.0 8 8.584659248261992E-5 0.0 0.0 0.0 0.0 9 8.584659248261992E-5 0.0 0.0 3.433863699304797E-5 0.0 10-11 8.584659248261992E-5 0.0 0.0 3.433863699304797E-5 0.0 12-13 8.584659248261992E-5 0.0 0.0 5.150795548957196E-5 0.0 14-15 8.584659248261992E-5 0.0 0.0 5.150795548957196E-5 0.0 16-17 8.584659248261992E-5 0.0 0.0 5.150795548957196E-5 0.0 18-19 8.584659248261992E-5 0.0 0.0 1.116005702274059E-4 0.0 20-21 8.584659248261992E-5 0.0 0.0 1.8886250346176385E-4 0.0 22-23 8.584659248261992E-5 0.0 0.0 4.0347898466831366E-4 0.0 24-25 8.584659248261992E-5 0.0 0.0 8.498812655779373E-4 0.0 26-27 8.584659248261992E-5 0.0 0.0 0.001364960820473657 0.0 28-29 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.0036141415435182993 0.0 30-31 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.013469330360523068 0.0 32-33 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.036639325671582186 0.0 34-35 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.07283224906225474 1.7169318496523986E-5 36-37 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.13009192624816224 1.7169318496523986E-5 38-39 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.2169257545443323 1.7169318496523986E-5 40-41 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.3122154722000404 1.7169318496523986E-5 42-43 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.4038910483122302 1.7169318496523986E-5 44-45 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.5100403599169898 1.7169318496523986E-5 46-47 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.6395141906992772 1.7169318496523986E-5 48-49 8.584659248261992E-5 1.7169318496523986E-5 0.0 0.7794097978089546 1.7169318496523986E-5 50-51 1.0301591097914392E-4 1.7169318496523986E-5 0.0 0.9242415839863827 1.7169318496523986E-5 52-53 1.0301591097914392E-4 1.7169318496523986E-5 0.0 1.066918620692497 1.7169318496523986E-5 54-55 1.0301591097914392E-4 1.7169318496523986E-5 0.0 1.2249364434752557 1.7169318496523986E-5 56-57 1.0301591097914392E-4 1.7169318496523986E-5 0.0 1.4076094076190224 1.7169318496523986E-5 58-59 1.0301591097914392E-4 1.7169318496523986E-5 0.0 1.6159075796188516 1.7169318496523986E-5 60-61 1.116005702274059E-4 1.7169318496523986E-5 0.0 1.8213813987260021 1.7169318496523986E-5 62-63 1.201852294756679E-4 1.7169318496523986E-5 0.0 2.0315853650789455 1.7169318496523986E-5 64-65 1.201852294756679E-4 1.7169318496523986E-5 0.0 2.2479187781351477 1.7169318496523986E-5 66-67 1.6310852571697784E-4 1.7169318496523986E-5 0.0 2.4939465275310884 1.7169318496523986E-5 68-69 1.7169318496523985E-4 1.7169318496523986E-5 0.0 2.7640628307776516 1.7169318496523986E-5 70-71 1.8027784421350185E-4 1.7169318496523986E-5 0.0 3.047502525177518 1.7169318496523986E-5 72-73 2.232011404548118E-4 1.7169318496523986E-5 0.0 3.325310683110524 3.433863699304797E-5 74-75 2.232011404548118E-4 1.7169318496523986E-5 0.0 3.6030845024065377 3.433863699304797E-5 76-77 2.232011404548118E-4 1.7169318496523986E-5 0.0 3.9106728432717643 4.292329624130996E-5 78-79 2.232011404548118E-4 1.7169318496523986E-5 0.0 4.2481443829801915 5.150795548957196E-5 80-81 2.232011404548118E-4 1.7169318496523986E-5 0.0 4.602390346859723 5.150795548957196E-5 82-83 2.232011404548118E-4 1.7169318496523986E-5 0.0 4.965418417150226 5.150795548957196E-5 84-85 2.232011404548118E-4 1.7169318496523986E-5 0.0 5.323218429958538 5.150795548957196E-5 86-87 2.232011404548118E-4 1.7169318496523986E-5 0.0 5.703604681249027 5.150795548957196E-5 88 2.232011404548118E-4 1.7169318496523986E-5 0.0 6.0104118181226625 5.150795548957196E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10250 0.0 47.93241 1 GTATCAA 17175 0.0 38.132137 1 TACCTGG 4120 0.0 31.827465 2 CAACGCA 20070 0.0 31.511003 5 ATCAACG 20195 0.0 31.164606 3 GTACTAG 3850 0.0 30.8956 1 TATCAAC 21575 0.0 30.21487 2 GTGGTAT 3860 0.0 29.11035 1 AACGCAG 22150 0.0 28.976206 6 TCAACGC 22715 0.0 28.121058 4 GTACCTG 5075 0.0 26.86574 1 GTACATG 22670 0.0 26.815372 1 TACATGG 22690 0.0 26.265146 2 ACGCAGA 24610 0.0 25.736107 7 TAGTACT 5590 0.0 25.561522 4 CGCAGAG 24975 0.0 25.284513 8 ACCTGGG 4935 0.0 25.239668 3 ACATGGG 22630 0.0 25.111153 3 GGGCACC 4410 0.0 24.83716 94 TCTAACG 1785 0.0 24.48724 2 >>END_MODULE