FastQCFastQC Report
Mon 6 Jun 2016
ERR1211232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6671020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184130.2760147623601788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148680.22287446297567687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87410.13102943777713152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111700.046.2249221
GTATCAA192250.037.6296581
GTACTAG45300.032.6252751
CAACGCA218550.031.7057615
ATCAACG219000.031.6189063
TATCAAC239650.029.9517122
CTGTGCG58950.029.8215649
GCATCGT44650.029.5824438
AACGCAG243400.029.1447356
GGGCACC55050.028.4289794
TCAACGC249250.028.1587164
TAACGCC23150.027.0077784
CTAGTAC71450.026.8439333
CTAACGC23300.026.833913
TACCTGG45500.026.482432
TCTAACG23800.026.3029172
ACGCAGA263600.026.2875467
TAGTACT68500.026.28434
CGCCTAC23850.026.0185787
GTACATG256000.026.015921
Produced by FastQC (version 0.11.5)