FastQCFastQC Report
Mon 6 Jun 2016
ERR1211232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6671020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170050.25490854472029767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146320.21933677308717406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85460.12810634655569914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115750.046.3845251
GTATCAA192850.036.5434881
GTACTAG46550.033.026011
CAACGCA219400.030.7542325
ATCAACG222500.030.3132633
TATCAAC240050.029.0527762
GGGCACC56250.028.33063994
AACGCAG245400.028.1851466
TAGGCAT59450.028.137485
CTGTGCG63950.027.8470529
TCAACGC251450.027.103614
TACCTGG45200.026.9287852
GCATCGT53100.026.458018
CTAGGCA67100.025.9173154
TAGTACT70600.025.7642234
GTACATG265600.025.383361
TACATGG265850.024.8898012
ACGCAGA273100.024.8274257
GTGGTAT49100.024.7996921
ACATGGG259700.024.5232543
Produced by FastQC (version 0.11.5)