FastQCFastQC Report
Mon 6 Jun 2016
ERR1211231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5568643
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171580.30811815374050733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134260.24110003101294156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83280.14955169508980912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84950.048.9247171
GTATCAA153400.040.4716031
ATCAACG181350.032.949693
CAACGCA182750.032.82595
TACCTGG44250.032.6554832
TATCAAC195000.031.5721472
AACGCAG201550.030.4168516
GCATCGT28950.030.2020938
TCAACGC200200.030.0586364
GTACTAG33800.029.5222971
GTGGTAT36050.029.3770561
GTACCTG51450.028.2685871
ACGCAGA220950.027.0655277
GTACATG196000.026.9443551
CGCAGAG224900.026.5243558
ACCTGGG54600.026.3482723
TACATGG198550.025.839712
ACATGGG198100.025.1561833
TGGTATC41350.024.2457222
CTGTGCG39350.024.1303529
Produced by FastQC (version 0.11.5)