##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211231_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5568643 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.310932663487318 31.0 31.0 34.0 28.0 34.0 2 31.319658487714154 31.0 31.0 34.0 28.0 34.0 3 31.38482122125624 31.0 31.0 34.0 28.0 34.0 4 34.976795603524955 37.0 35.0 37.0 32.0 37.0 5 35.03225902612181 37.0 35.0 37.0 32.0 37.0 6 35.09233308725303 37.0 35.0 37.0 32.0 37.0 7 35.00565523773027 37.0 35.0 37.0 32.0 37.0 8 35.00647787979944 37.0 35.0 37.0 32.0 37.0 9 36.556377918282784 39.0 37.0 39.0 32.0 39.0 10-11 36.50232695829128 39.0 37.0 39.0 32.0 39.0 12-13 36.398998373571445 39.0 37.0 39.0 32.0 39.0 14-15 37.580857760140134 40.0 37.0 41.0 32.0 41.0 16-17 37.553310743748526 40.0 37.0 41.0 32.0 41.0 18-19 37.5663483904427 40.0 37.0 41.0 32.0 41.0 20-21 37.52982503996036 40.0 37.0 41.0 31.5 41.0 22-23 37.423238210817246 40.0 37.0 41.0 31.0 41.0 24-25 37.332102991698335 40.0 37.0 41.0 31.0 41.0 26-27 37.189432506267686 40.0 36.0 41.0 31.0 41.0 28-29 37.06560655800704 40.0 36.0 41.0 30.0 41.0 30-31 36.91026090198277 39.0 36.0 41.0 30.0 41.0 32-33 36.72415182657606 39.0 35.5 41.0 30.0 41.0 34-35 36.57208345013318 39.0 35.0 41.0 30.0 41.0 36-37 36.46231029714062 39.0 35.0 41.0 29.5 41.0 38-39 36.325667402273766 39.0 35.0 41.0 29.0 41.0 40-41 36.16512712343025 39.0 35.0 40.0 28.0 41.0 42-43 35.95914040458331 38.5 35.0 40.0 27.5 41.0 44-45 35.74947325587221 38.0 34.5 40.0 27.0 41.0 46-47 35.53107390795208 38.0 34.0 40.0 26.0 41.0 48-49 35.336396856469335 38.0 34.0 40.0 26.0 41.0 50-51 34.67608481994626 37.5 33.5 39.5 24.5 40.5 52-53 34.79130858271934 38.0 33.5 39.5 25.0 40.5 54-55 35.28620805822891 38.0 34.0 40.0 26.0 41.0 56-57 35.259072991391264 38.0 34.0 40.0 26.0 41.0 58-59 35.062020675414104 38.0 34.0 40.0 26.0 41.0 60-61 34.843381502459394 37.5 34.0 40.0 26.0 41.0 62-63 34.55281538069508 37.0 33.0 40.0 25.0 41.0 64-65 34.30920926336991 36.5 33.0 40.0 25.0 41.0 66-67 34.139686993761316 36.0 33.0 39.5 25.0 41.0 68-69 33.84152835798596 36.0 33.0 39.0 24.0 41.0 70-71 33.528690472705826 35.0 32.5 39.0 24.0 41.0 72-73 33.18427541503379 35.0 32.0 38.0 23.5 40.0 74-75 32.84904661692265 35.0 32.0 37.0 23.0 39.5 76-77 32.50666347259107 35.0 31.5 37.0 22.5 39.0 78-79 32.16209550513473 35.0 31.0 36.0 22.0 39.0 80-81 31.85686845071591 35.0 31.0 36.0 21.0 38.5 82-83 31.56076686905589 35.0 31.0 35.5 20.0 37.0 84-85 31.302480873706575 34.0 31.0 35.0 20.0 37.0 86-87 31.07087058732262 34.0 30.0 35.0 20.0 36.5 88-89 30.85063686072172 34.0 30.0 35.0 19.5 36.0 90-91 30.622174827870992 34.0 30.0 35.0 19.0 36.0 92-93 30.429749671508837 34.0 29.5 35.0 18.0 35.5 94-95 30.25377107133641 34.0 29.0 35.0 18.0 35.0 96-97 30.076052549965944 34.0 29.0 35.0 17.5 35.0 98-99 29.841897658011117 34.0 29.0 35.0 17.0 35.0 100 28.708165705720408 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5764765517000647 1101 2 -0.45576342452710605 1101 3 -0.5917340689116131 1101 4 -0.5655727570524647 1101 5 -0.3861562362802502 1101 6 -0.3633991718527483 1101 7 -0.46046406837846376 1101 8 -0.3763579682279641 1101 9 -0.6823781258007955 1101 10-11 -0.6012913624490892 1101 12-13 -0.6206773380074821 1101 14-15 -0.7584841532311586 1101 16-17 -0.8275941199214287 1101 18-19 -0.8003678449648177 1101 20-21 -0.7300953754344164 1101 22-23 -0.8181917958007219 1101 24-25 -0.9019365768052836 1101 26-27 -0.8960990183435484 1101 28-29 -0.7947988084361626 1101 30-31 -0.9831741104368774 1101 32-33 -0.9342168334674454 1101 34-35 -0.8906876795675913 1101 36-37 -1.0964817127230404 1101 38-39 -1.0129406267167695 1101 40-41 -0.9432989294363026 1101 42-43 -1.1162715595177843 1101 44-45 -1.1030894569209124 1101 46-47 -1.16728310999477 1101 48-49 -1.1833875984762656 1101 50-51 -1.233879520109845 1101 52-53 -1.1849624717593201 1101 54-55 -1.061730537451588 1101 56-57 -1.0263901148116474 1101 58-59 -1.0723769276324475 1101 60-61 -1.0341715189156275 1101 62-63 -1.1115236831240765 1101 64-65 -1.0094674690621375 1101 66-67 -1.0581011966682823 1101 68-69 -0.9974441124850841 1101 70-71 -1.029579701497397 1101 72-73 -0.9792035496288918 1101 74-75 -1.06044811266581 1101 76-77 -1.0929111774359797 1101 78-79 -0.9995253379112725 1101 80-81 -1.0391727911002278 1101 82-83 -1.0143907241926513 1101 84-85 -1.1565150598252316 1101 86-87 -1.1832581953961459 1101 88-89 -1.3327684326619327 1101 90-91 -1.3695392949746399 1101 92-93 -1.4397468054014766 1101 94-95 -1.5299694253861134 1101 96-97 -1.4774346129535978 1101 98-99 -1.6142378232308623 1101 100 -1.7523404240423162 1102 1 -0.44736318683848353 1102 2 -0.37757111011151423 1102 3 -0.45842205234544053 1102 4 -0.21217326659834157 1102 5 -0.29291776453821683 1102 6 -0.38467587614607623 1102 7 -0.29205156243627073 1102 8 -0.32888212657159954 1102 9 -0.491487345955143 1102 10-11 -0.5400815706573496 1102 12-13 -0.6400280387714901 1102 14-15 -0.784663812843128 1102 16-17 -0.7838995524145105 1102 18-19 -0.7060833685544523 1102 20-21 -0.8272394427685086 1102 22-23 -0.7495687917264107 1102 24-25 -0.741556655855284 1102 26-27 -0.8787757042862694 1102 28-29 -0.9567562294790903 1102 30-31 -0.8811674472663142 1102 32-33 -0.8977563818050882 1102 34-35 -0.8726070863682338 1102 36-37 -0.8654311322662878 1102 38-39 -0.9966190820698131 1102 40-41 -1.0788651249193677 1102 42-43 -1.0576346303345403 1102 44-45 -1.0919614914580222 1102 46-47 -1.0456923887603367 1102 48-49 -1.1308442985457958 1102 50-51 -1.1823451676847512 1102 52-53 -1.0432460527863654 1102 54-55 -0.9191251222808532 1102 56-57 -0.927829945764536 1102 58-59 -0.9495942273556821 1102 60-61 -0.9738279432513863 1102 62-63 -0.9746174776905576 1102 64-65 -0.9359510211395161 1102 66-67 -1.003124562059817 1102 68-69 -1.1262745852585567 1102 70-71 -1.067699815745776 1102 72-73 -1.021447252086368 1102 74-75 -1.0567414076526944 1102 76-77 -1.1333546215611356 1102 78-79 -1.185313441335957 1102 80-81 -1.2751096768589676 1102 82-83 -1.2908148147497585 1102 84-85 -1.2322309314437163 1102 86-87 -1.326832495205604 1102 88-89 -1.2350081367424934 1102 90-91 -1.336173927167465 1102 92-93 -1.378569957704439 1102 94-95 -1.4724250101813645 1102 96-97 -1.429808762952451 1102 98-99 -1.5943190246897387 1102 100 -1.5545597407509462 1103 1 -0.4965071733720663 1103 2 -0.486023207572444 1103 3 -0.5957210634422943 1103 4 -0.5579844720758871 1103 5 -0.42193732201705103 1103 6 -0.45894939458053585 1103 7 -0.4316034727438236 1103 8 -0.6188214316237577 1103 9 -0.7489580401284854 1103 10-11 -0.6927630403162439 1103 12-13 -0.7057354422610302 1103 14-15 -0.89186583555054 1103 16-17 -0.8914144124318213 1103 18-19 -0.8110100414889772 1103 20-21 -0.7488398247221753 1103 22-23 -0.8717662213821313 1103 24-25 -0.8714964903093545 1103 26-27 -0.8759738937317607 1103 28-29 -0.9836566107465003 1103 30-31 -0.914138401728934 1103 32-33 -1.0030612829883054 1103 34-35 -1.0477428436433485 1103 36-37 -0.9925356273786363 1103 38-39 -1.0101662807004246 1103 40-41 -0.9935573022219444 1103 42-43 -1.2543566521792329 1103 44-45 -1.2914297366573777 1103 46-47 -1.217748301444317 1103 48-49 -1.2356649930622723 1103 50-51 -1.3389072513322162 1103 52-53 -1.193560801825356 1103 54-55 -0.9262548914322153 1103 56-57 -0.9220246617453256 1103 58-59 -0.9161356349177012 1103 60-61 -0.8762062322832591 1103 62-63 -0.8377916930728162 1103 64-65 -0.9159646287291778 1103 66-67 -0.8917085982395392 1103 68-69 -0.895031441748479 1103 70-71 -0.8515849198467791 1103 72-73 -0.8829599297913902 1103 74-75 -0.8580500107277302 1103 76-77 -0.9626456445714879 1103 78-79 -1.0641699136173557 1103 80-81 -1.0045846180120215 1103 82-83 -0.9344516489655916 1103 84-85 -1.0088935230228913 1103 86-87 -1.0930264582592315 1103 88-89 -1.1015905239333215 1103 90-91 -1.180715156301435 1103 92-93 -1.2757005993885642 1103 94-95 -1.4156580283898492 1103 96-97 -1.3950835274421287 1103 98-99 -1.4484299775249312 1103 100 -1.574420351190554 1104 1 -0.5950328552427493 1104 2 -0.49595631889956593 1104 3 -0.5403396608033333 1104 4 -0.40073869011637697 1104 5 -0.3498335186103674 1104 6 -0.39144915815632686 1104 7 -0.4787989777381867 1104 8 -0.49006170528672044 1104 9 -0.5102910540953474 1104 10-11 -0.501058429501505 1104 12-13 -0.5725604550124359 1104 14-15 -0.7227758982300898 1104 16-17 -0.9058091199401872 1104 18-19 -0.8434198669691497 1104 20-21 -0.7763775479492665 1104 22-23 -0.8886873153828461 1104 24-25 -0.8616287638420843 1104 26-27 -0.812414596989143 1104 28-29 -0.8971917418340354 1104 30-31 -0.9251845478592458 1104 32-33 -0.8678989564500412 1104 34-35 -0.9670881384914694 1104 36-37 -0.9300204923532789 1104 38-39 -1.0706961306136833 1104 40-41 -0.9449328841743281 1104 42-43 -1.0153242550857726 1104 44-45 -1.0365028111178276 1104 46-47 -1.1548076415959656 1104 48-49 -1.0095877707134377 1104 50-51 -1.1319718245981463 1104 52-53 -0.9207339085325117 1104 54-55 -0.7699583333036415 1104 56-57 -0.7676237025606696 1104 58-59 -0.7619958857289078 1104 60-61 -0.8011656332075532 1104 62-63 -0.7578783716149573 1104 64-65 -0.7590804982974291 1104 66-67 -0.86374595093951 1104 68-69 -0.922994689798557 1104 70-71 -0.8574384538848889 1104 72-73 -0.8998778262515401 1104 74-75 -0.8972986584587588 1104 76-77 -0.9080475037184712 1104 78-79 -0.96591540256588 1104 80-81 -0.9238662914923594 1104 82-83 -0.8495726051426189 1104 84-85 -0.9116535625251139 1104 86-87 -1.0174651445467795 1104 88-89 -1.0523429149185013 1104 90-91 -1.0174772802125247 1104 92-93 -1.0836086985844133 1104 94-95 -1.105755187113438 1104 96-97 -1.2266128277659938 1104 98-99 -1.1470090873916483 1104 100 -1.1725981794054974 1105 1 -0.650159812512058 1105 2 -0.5967334998111653 1105 3 -0.6378884938569449 1105 4 -0.49380178635511385 1105 5 -0.45104659892355414 1105 6 -0.39777819843384066 1105 7 -0.5174087586773481 1105 8 -0.5586899078525178 1105 9 -0.7762015533729922 1105 10-11 -0.7350416077317732 1105 12-13 -0.7497836788564172 1105 14-15 -0.9078992140681308 1105 16-17 -0.9469998451508488 1105 18-19 -0.9089142368965852 1105 20-21 -0.8940433494337654 1105 22-23 -0.8820035435719049 1105 24-25 -0.9307454566558775 1105 26-27 -0.9020896905118647 1105 28-29 -0.992797907808459 1105 30-31 -1.0168983637598004 1105 32-33 -1.0432007891212507 1105 34-35 -1.0078608178091457 1105 36-37 -1.1131819756843768 1105 38-39 -1.1297455550576387 1105 40-41 -1.0870450443644515 1105 42-43 -1.1166438860509018 1105 44-45 -1.2466647316032038 1105 46-47 -1.2746747293544658 1105 48-49 -1.281199585469544 1105 50-51 -1.1748169939520778 1105 52-53 -1.026936673738689 1105 54-55 -1.0105501617128212 1105 56-57 -0.8905112275099469 1105 58-59 -0.9646515751686664 1105 60-61 -0.9119028473574886 1105 62-63 -1.034717727912124 1105 64-65 -0.9626447202768915 1105 66-67 -0.9733667789224896 1105 68-69 -0.9845633983133979 1105 70-71 -0.8867194058225536 1105 72-73 -0.9284064781097143 1105 74-75 -0.8497136962922269 1105 76-77 -0.8949026441143566 1105 78-79 -0.9780332576259632 1105 80-81 -0.9455674878713687 1105 82-83 -1.1359952457187532 1105 84-85 -1.0952067963259928 1105 86-87 -1.19736023436419 1105 88-89 -1.1850447359991954 1105 90-91 -1.1791832382876954 1105 92-93 -1.1529319331062737 1105 94-95 -1.198086004299899 1105 96-97 -1.2014096057837946 1105 98-99 -1.0563870163937814 1105 100 -1.118155359459216 1106 1 -0.5610702731857877 1106 2 -0.5752978703553744 1106 3 -0.6152507229908011 1106 4 -0.463986842898926 1106 5 -0.5732289717988124 1106 6 -0.4534085114997808 1106 7 -0.43362736009961367 1106 8 -0.41794682384423254 1106 9 -0.6109954712088239 1106 10-11 -0.5988945770763721 1106 12-13 -0.7067930111452156 1106 14-15 -0.8586367619186674 1106 16-17 -0.9322574872293501 1106 18-19 -0.9976351819940916 1106 20-21 -0.8463457810266917 1106 22-23 -0.9087608992085308 1106 24-25 -0.856895216063883 1106 26-27 -0.9730066443448422 1106 28-29 -0.9971497620750895 1106 30-31 -1.0495811129440042 1106 32-33 -0.9360213999606728 1106 34-35 -0.9595427225514754 1106 36-37 -0.9166827534221511 1106 38-39 -0.9009414355029719 1106 40-41 -1.0386482966040447 1106 42-43 -0.9968035325677391 1106 44-45 -0.968958775979246 1106 46-47 -0.9098662449998614 1106 48-49 -0.9738987816823581 1106 50-51 -0.9010357793986259 1106 52-53 -0.9379838731055798 1106 54-55 -0.8426448310262842 1106 56-57 -0.756349852670219 1106 58-59 -0.7608903184055364 1106 60-61 -0.7124706958476281 1106 62-63 -0.7801626848773537 1106 64-65 -0.7809664708061277 1106 66-67 -0.893519529609371 1106 68-69 -0.8305857323877603 1106 70-71 -0.8908018846754757 1106 72-73 -0.8377167554967784 1106 74-75 -0.8188062921525976 1106 76-77 -0.886218993850953 1106 78-79 -0.8641588093742101 1106 80-81 -0.753168827011141 1106 82-83 -0.8461356908640703 1106 84-85 -0.8538619679858357 1106 86-87 -0.9666364668837168 1106 88-89 -1.038587867545207 1106 90-91 -1.0197211596887072 1106 92-93 -1.0297341150245458 1106 94-95 -1.1827793617121891 1106 96-97 -1.3084146987403287 1106 98-99 -1.0304727478475648 1106 100 -1.2931202226766807 1107 1 -0.6780936453235782 1107 2 -0.652807914201027 1107 3 -0.6349926986623942 1107 4 -0.5411575348859046 1107 5 -0.47671859074722533 1107 6 -0.5145158582011291 1107 7 -0.5693406875481841 1107 8 -0.6147622441821241 1107 9 -0.7102202601443253 1107 10-11 -0.7316665097621637 1107 12-13 -0.6886014835016923 1107 14-15 -0.8562198788899167 1107 16-17 -0.8526410532461242 1107 18-19 -0.9181507407884268 1107 20-21 -0.9189406987257271 1107 22-23 -0.9457561900910108 1107 24-25 -0.9640135060857062 1107 26-27 -1.045585967101836 1107 28-29 -1.144036745252599 1107 30-31 -1.0538133360235236 1107 32-33 -1.135659504808551 1107 34-35 -1.1111714375793156 1107 36-37 -1.1755756072536272 1107 38-39 -1.1240568468797818 1107 40-41 -1.0321715118304482 1107 42-43 -1.1129056561659567 1107 44-45 -1.004613530823434 1107 46-47 -0.8881522953815448 1107 48-49 -0.9539742545420964 1107 50-51 -1.018337252086532 1107 52-53 -0.8995546060278983 1107 54-55 -0.7979270284398225 1107 56-57 -0.7689684362025346 1107 58-59 -0.716482583225492 1107 60-61 -0.8003646401780884 1107 62-63 -0.8166264342773246 1107 64-65 -0.762911357281979 1107 66-67 -0.7600852651204875 1107 68-69 -0.714553610285023 1107 70-71 -0.7727742944666005 1107 72-73 -0.6621478804999796 1107 74-75 -0.6291337067583598 1107 76-77 -0.6709684465850287 1107 78-79 -0.666852046615567 1107 80-81 -0.7777379459772042 1107 82-83 -0.7631134362342955 1107 84-85 -0.795665584636037 1107 86-87 -0.7328845650592619 1107 88-89 -0.7321245946881803 1107 90-91 -0.7896031938492882 1107 92-93 -0.9312403089359407 1107 94-95 -0.9953264934009098 1107 96-97 -0.9903850477051854 1107 98-99 -0.9142874697244459 1107 100 -0.8180764587924649 1108 1 -0.6340779527104239 1108 2 -0.6897540676247687 1108 3 -0.6914487447115398 1108 4 -0.6001295396343451 1108 5 -0.4900119004706198 1108 6 -0.630258008516364 1108 7 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1.3238195579963374 2311 66-67 1.2552215287620072 2311 68-69 1.2526982674933862 2311 70-71 1.3021398192764195 2311 72-73 1.2828151624364068 2311 74-75 1.209672505040686 2311 76-77 1.1615608773599106 2311 78-79 1.1470856801095408 2311 80-81 1.1568169391224892 2311 82-83 1.2140211288475484 2311 84-85 1.2181541941378384 2311 86-87 1.170679021162794 2311 88-89 1.322275328521254 2311 90-91 1.4022498428693062 2311 92-93 1.3149722111111863 2311 94-95 1.4084244900334077 2311 96-97 1.449443982453431 2311 98-99 1.4934773724118493 2311 100 1.545089246835186 2312 1 0.6209708378441405 2312 2 0.6122679373473829 2312 3 0.6098909181883911 2312 4 0.27179410620345834 2312 5 0.4312454057269619 2312 6 0.5673373120747982 2312 7 0.37767515746659086 2312 8 0.5358122845464592 2312 9 0.7295414032096161 2312 10-11 0.6717919134936636 2312 12-13 0.8316835854902962 2312 14-15 1.0072455796482913 2312 16-17 1.0616591774143984 2312 18-19 1.003496911787046 2312 20-21 0.9887640054603963 2312 22-23 1.065223063684222 2312 24-25 1.0017676106271907 2312 26-27 1.070894047007009 2312 28-29 1.1461018865203911 2312 30-31 1.1147359447926846 2312 32-33 1.143416520954581 2312 34-35 1.1592003618123101 2312 36-37 1.2278834104432619 2312 38-39 1.2327336910568931 2312 40-41 1.2782739222794177 2312 42-43 1.2840218752061716 2312 44-45 1.3021688995172127 2312 46-47 1.3366048667487433 2312 48-49 1.3718298690694084 2312 50-51 1.3130069606586403 2312 52-53 1.2580729223111362 2312 54-55 1.2208723438768274 2312 56-57 1.1213689331632395 2312 58-59 1.0585414568406506 2312 60-61 1.0465650195829923 2312 62-63 1.0717995606241715 2312 64-65 1.1669487594291255 2312 66-67 1.1034792185981743 2312 68-69 1.1098022954346618 2312 70-71 1.0986275465840407 2312 72-73 1.0731266837036841 2312 74-75 1.1095678536175129 2312 76-77 1.13317019991851 2312 78-79 1.0941522606753153 2312 80-81 1.0462881319488595 2312 82-83 0.9901497406825541 2312 84-85 1.131940614578042 2312 86-87 1.110977343906118 2312 88-89 1.053859329073969 2312 90-91 1.1230220614051483 2312 92-93 1.2032427004598816 2312 94-95 1.2088978483049182 2312 96-97 1.222424545169968 2312 98-99 0.8764308870618578 2312 100 1.207126971984934 2313 1 0.6944497109574499 2313 2 0.6674054825173066 2313 3 0.6471706584881076 2313 4 0.5366886560896944 2313 5 0.41751784650327295 2313 6 0.6006779702498761 2313 7 0.5962817340763706 2313 8 0.6041356584304367 2313 9 0.771472263354056 2313 10-11 0.809282924284247 2313 12-13 0.8608817603055314 2313 14-15 1.0869696884632063 2313 16-17 1.1279978957901164 2313 18-19 1.0136540282505067 2313 20-21 1.0037727894706236 2313 22-23 1.0383487436312961 2313 24-25 1.0624187386381365 2313 26-27 1.0764820189054163 2313 28-29 1.0612850446058033 2313 30-31 1.116400192606683 2313 32-33 1.1678264903364024 2313 34-35 1.1875148577282602 2313 36-37 1.212028472654687 2313 38-39 1.2994583787910514 2313 40-41 1.280405534745121 2313 42-43 1.2839049267457696 2313 44-45 1.278851624538369 2313 46-47 1.3350198102796398 2313 48-49 1.3001351733606654 2313 50-51 1.336501723079735 2313 52-53 1.2543594317018787 2313 54-55 1.105294414559161 2313 56-57 1.104016366057536 2313 58-59 1.09204135608541 2313 60-61 1.0608855105169042 2313 62-63 1.0785295721696144 2313 64-65 1.088361609580268 2313 66-67 1.1126449758848125 2313 68-69 1.1146482907143138 2313 70-71 1.0818269525480346 2313 72-73 1.1238393153298532 2313 74-75 1.05678525048166 2313 76-77 0.9295308570922884 2313 78-79 1.066251503867278 2313 80-81 1.127024027062074 2313 82-83 1.1321605739935805 2313 84-85 1.095477576346589 2313 86-87 1.1981769092039833 2313 88-89 1.2154489493401606 2313 90-91 1.17322762250976 2313 92-93 1.274370482648905 2313 94-95 1.3064866870278173 2313 96-97 1.36630410630492 2313 98-99 1.3502021614854947 2313 100 1.519759275585642 2314 1 0.6327733934487014 2314 2 0.6300741061180375 2314 3 0.6637744258751326 2314 4 0.49806814861295123 2314 5 0.6163278091651847 2314 6 0.632890142172954 2314 7 0.6553668324218833 2314 8 0.5803738283168869 2314 9 0.7417295877135999 2314 10-11 0.76605754624984 2314 12-13 0.8522677678315489 2314 14-15 1.0194105013968553 2314 16-17 1.0120614143295938 2314 18-19 0.928817423424654 2314 20-21 1.0336069397646241 2314 22-23 1.0725332778907202 2314 24-25 1.083738606111659 2314 26-27 1.0557749859873127 2314 28-29 1.1079083980620439 2314 30-31 1.1359865583666036 2314 32-33 1.0835286735010428 2314 34-35 1.161491345320556 2314 36-37 1.106403283318123 2314 38-39 1.1174997809948337 2314 40-41 1.2416199622825914 2314 42-43 1.3191334810721216 2314 44-45 1.3364600117375716 2314 46-47 1.3309488916460026 2314 48-49 1.2731668258719537 2314 50-51 1.3359521223745858 2314 52-53 1.303301450914752 2314 54-55 1.1553715406947234 2314 56-57 1.057172401286003 2314 58-59 1.0059378159022643 2314 60-61 1.0527208844269609 2314 62-63 1.0631180548491912 2314 64-65 1.083954675573409 2314 66-67 1.0836076816001352 2314 68-69 1.088410052392959 2314 70-71 1.094046871302119 2314 72-73 1.1213521172190255 2314 74-75 1.0943946550797605 2314 76-77 1.092701701633743 2314 78-79 1.167903522423174 2314 80-81 1.1565405253484649 2314 82-83 1.1310994908241838 2314 84-85 1.1903982466071454 2314 86-87 1.215294805204831 2314 88-89 1.230294030969315 2314 90-91 1.3552494085329805 2314 92-93 1.4092441107572213 2314 94-95 1.3439361136345767 2314 96-97 1.3640374510744842 2314 98-99 1.4805105884681886 2314 100 1.459730186189585 2315 1 0.42386248389177794 2315 2 0.4397247515381295 2315 3 0.48624668413445704 2315 4 0.2403479263279209 2315 5 0.3650113382692197 2315 6 0.42705044212257093 2315 7 0.44314108987718015 2315 8 0.4567196823727713 2315 9 0.5897455112357477 2315 10-11 0.5288036603872897 2315 12-13 0.5620332687101879 2315 14-15 0.7985666824374889 2315 16-17 0.7457611118520191 2315 18-19 0.6002348942697111 2315 20-21 0.6699318108649379 2315 22-23 0.5662046960418294 2315 24-25 0.7658846279082994 2315 26-27 0.8316657411981652 2315 28-29 0.8339317944305549 2315 30-31 0.8147059638919529 2315 32-33 0.8813993160596212 2315 34-35 0.8851965990909889 2315 36-37 0.950823544137009 2315 38-39 1.0231443979297197 2315 40-41 1.062482773866975 2315 42-43 1.101053687030742 2315 44-45 1.079838761327217 2315 46-47 1.1065442133378411 2315 48-49 1.0802730410576302 2315 50-51 1.036989541027772 2315 52-53 1.0738260489425926 2315 54-55 0.9253484137998527 2315 56-57 0.894999039325846 2315 58-59 0.9467988302642425 2315 60-61 0.8420379772189364 2315 62-63 0.8635139814084098 2315 64-65 0.8511786494230549 2315 66-67 0.8543921307767022 2315 68-69 0.8647943196089898 2315 70-71 0.8603537131237218 2315 72-73 0.5946902021830951 2315 74-75 0.7615257834106544 2315 76-77 0.8832533369964111 2315 78-79 0.8599929788939704 2315 80-81 0.5723593192476493 2315 82-83 0.53186148898439 2315 84-85 0.8574460970501576 2315 86-87 0.9851466956458026 2315 88-89 1.0585631324371114 2315 90-91 1.1145473463135325 2315 92-93 1.1819005349385705 2315 94-95 1.1782431472476844 2315 96-97 1.1374387789563194 2315 98-99 0.5181554744378296 2315 100 0.8822031466603448 2316 1 0.2629845223243912 2316 2 0.21810371269600992 2316 3 0.2338013344573291 2316 4 0.08069890861980156 2316 5 0.07660802957288126 2316 6 0.12523725574573064 2316 7 0.09159991641663368 2316 8 0.13330914779384528 2316 9 0.2911011259994396 2316 10-11 0.13665385327890078 2316 12-13 -0.004407808882895381 2316 14-15 -0.013924199967853212 2316 16-17 0.12583582476754884 2316 18-19 0.22310363224096363 2316 20-21 0.28905985331455497 2316 22-23 0.13071434338492338 2316 24-25 0.24441389018634396 2316 26-27 0.3389726462968028 2316 28-29 0.27638645721810207 2316 30-31 0.19245960695907627 2316 32-33 0.36608109884222273 2316 34-35 0.4275356766744096 2316 36-37 0.4181479515645705 2316 38-39 0.5854798172536135 2316 40-41 0.642300528147949 2316 42-43 0.5909158712965308 2316 44-45 0.5038062331159807 2316 46-47 0.3338870661449391 2316 48-49 0.31364978852827363 2316 50-51 0.380888562447673 2316 52-53 0.41561087819734155 2316 54-55 0.14263546606445487 2316 56-57 0.034385578639962944 2316 58-59 0.11095533859968754 2316 60-61 0.0915821945965476 2316 62-63 0.3532985636141106 2316 64-65 0.2946813500438026 2316 66-67 0.24965254827061045 2316 68-69 0.2180746229976478 2316 70-71 0.020227422892368452 2316 72-73 -0.09404251819466225 2316 74-75 0.20741105718105501 2316 76-77 0.3221558928060162 2316 78-79 0.30997815983538146 2316 80-81 0.5293080765961058 2316 82-83 0.5030142826930835 2316 84-85 0.5747702131462908 2316 86-87 0.6952125805717024 2316 88-89 0.5546377327908196 2316 90-91 0.4224664401666196 2316 92-93 0.3955085999937893 2316 94-95 0.7071891256827705 2316 96-97 0.6465111848722387 2316 98-99 0.4062837641091406 2316 100 0.35371501174774167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18.0 1 347.0 2 112.0 3 136.0 4 77.0 5 80.0 6 107.0 7 510.0 8 1768.0 9 2976.0 10 4473.0 11 6750.0 12 9588.0 13 12546.0 14 15000.0 15 17816.0 16 21003.0 17 25561.0 18 31255.0 19 37090.0 20 44534.0 21 52754.0 22 58547.0 23 59989.0 24 60561.0 25 62246.0 26 68586.0 27 77550.0 28 90840.0 29 107975.0 30 129961.0 31 159599.0 32 200206.0 33 260886.0 34 362576.0 35 500868.0 36 745048.0 37 1070748.0 38 997458.0 39 270164.0 40 334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11474362383692 19.523679811176738 12.912925107485226 23.44865145750111 2 15.675799604041583 19.84035790900868 41.192082989432116 23.291759497517617 3 19.45760075579192 25.578940562210047 28.933449719541564 26.03000896245647 4 12.597412464161534 16.116303868156475 38.57784133494477 32.708442332737214 5 13.258741268789537 37.475274031001554 34.72367534095479 14.54230935925412 6 30.79985798700897 36.94650149271752 18.133247857228074 14.12039266304543 7 27.622532236152107 31.978471319199492 22.294482008792517 18.104514435855883 8 25.931755106616812 35.621206902716146 20.01291083671241 18.43412715395463 9 26.14026533791708 16.10973538068848 21.03417759905259 36.71582168234185 10-11 25.36773615659464 26.20795257564867 27.74238501098461 20.68192625677208 12-13 25.590961087349896 23.82392318882433 28.132209861799705 22.452905862026064 14-15 22.835576881196808 25.268648747919336 26.084623940247493 25.81115043063636 16-17 22.05996179209154 27.99630475481283 27.022939742304512 22.920793710791116 18-19 21.632635247020144 27.505465254023964 29.12784846309684 21.734051035859054 20-21 22.707461150989715 26.79906355726102 28.892112585793285 21.601362705955975 22-23 22.723007081380416 26.619692770457277 28.58559828624972 22.071701861912587 24-25 22.254920275107906 26.6064336739551 29.106572880424338 22.032073170512657 26-27 22.222623354769077 26.831995541321934 28.6003344906606 22.345046613248385 28-29 21.87010815833575 27.14954106549391 28.82042422221542 22.15992655395492 30-31 22.531419811790947 26.888399163395594 28.742839336212416 21.83734168860104 32-33 22.445577461191295 27.020850803152925 28.358886681870217 22.174685053785563 34-35 22.558633383791417 26.979812127093293 28.700835364903536 21.760719124211754 36-37 22.400723292615943 26.799699604010407 28.437040959399123 22.36253614397453 38-39 22.34548619203153 27.033139285980823 28.666470706411573 21.954903815576074 40-41 22.43853362650122 26.463065750280524 28.888921620101627 22.20947900311663 42-43 22.326006592324426 26.58571557653558 28.524907679973527 22.563370151166463 44-45 22.347245810613398 27.120307547008633 28.42768954294718 22.104757099430792 46-47 22.308897442922785 26.933025507889525 28.0205805863356 22.737496462852086 48-49 22.5684863769198 26.873073597490944 28.099063685517333 22.45937634007192 50-51 22.48816285561167 27.00034694807392 27.944300778383347 22.567189417931065 52-53 22.270485946276843 27.305250289347306 27.758037836791505 22.666225927584353 54-55 22.15921982254617 27.174093271136528 27.90273865826565 22.763948248051648 56-57 22.619361999039974 27.053646879769595 27.584446058860557 22.742545062329874 58-59 22.191191924824718 27.188286464958555 28.04890263084922 22.571618979367507 60-61 22.34944518895835 27.45829240303043 27.70464657502949 22.48761583298173 62-63 22.436545515339425 27.126294301923654 27.57508951700201 22.862070665734908 64-65 22.051970047408382 27.099951870438343 27.756297549721232 23.09178053243204 66-67 22.28747721232335 26.9425806683978 27.629599311427523 23.140342807851326 68-69 22.017835973500777 26.988164741849594 27.822485679069963 23.171513605579662 70-71 22.07383587237712 26.680763150330556 27.87242203219316 23.372978945099167 72-73 22.31052120031835 26.86222532827661 27.823448355158554 23.003805116246482 74-75 22.406595626224977 26.970385226553113 27.57721581810045 23.045803329121465 76-77 22.21682486491564 26.900006726490222 27.48812109909593 23.395047309498207 78-79 22.128953698089997 26.917930693653492 27.558633666313042 23.39448194194347 80-81 21.96157041307989 27.101714018851062 27.710157032327015 23.226558535742033 82-83 22.444746252108306 26.660495205847223 28.1585293099935 22.736229232050967 84-85 22.51961803625491 26.855440670133763 27.56968751408729 23.055253779524037 86-87 21.902919700818895 27.125280515960785 27.81246492147349 23.159334861746828 88-89 22.31938302669636 26.541977499768077 27.749959745001085 23.388679728534477 90-91 22.326849386815685 26.52895539716792 27.794102696832724 23.350092519183676 92-93 22.412274454730014 26.63431019999235 27.73731523109842 23.21610011417922 94-95 21.81407017800683 26.62753369313995 28.03165064840182 23.5267454804514 96-97 21.996797000187346 26.530985233186545 27.839011539025964 23.63320622760015 98-99 21.814245878293807 26.65879544007737 27.658748377974074 23.86821030365475 100 22.238107504259926 26.385136118245416 27.32433171342975 24.052424664064905 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1052.0 1 766.5 2 995.0 3 1455.0 4 1059.0 5 810.5 6 1170.0 7 2046.0 8 3273.0 9 3974.0 10 3667.0 11 3266.0 12 3495.0 13 3957.0 14 4436.0 15 4978.0 16 5529.5 17 6098.5 18 6737.5 19 7360.5 20 8146.5 21 9019.0 22 10242.5 23 12205.0 24 14582.5 25 17457.0 26 21736.5 27 26811.0 28 32958.5 29 42067.5 30 54036.0 31 66572.0 32 81502.5 33 97157.5 34 110876.0 35 124574.5 36 138936.0 37 157301.0 38 168604.0 39 169997.5 40 168649.5 41 167154.0 42 170065.0 43 177431.0 44 188071.0 45 204186.0 46 219303.5 47 230454.5 48 238298.0 49 238479.0 50 232561.0 51 230570.5 52 226369.0 53 215324.5 54 203049.5 55 181023.0 56 160031.0 57 137435.5 58 114585.5 59 101201.5 60 82995.0 61 63076.0 62 48824.5 63 38653.0 64 28681.5 65 18765.5 66 13069.0 67 9786.5 68 7529.5 69 5527.5 70 4196.0 71 3279.0 72 2526.0 73 1932.0 74 1459.0 75 1054.5 76 731.0 77 502.5 78 344.5 79 238.0 80 172.5 81 124.5 82 98.5 83 92.5 84 75.0 85 51.5 86 44.5 87 37.0 88 38.5 89 34.0 90 21.5 91 16.5 92 9.0 93 6.5 94 7.0 95 8.0 96 5.5 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057949486077667395 2 0.13383871079543078 3 0.01747283853534874 4 0.017095726912283657 5 0.020040789111458575 6 0.05306499267415778 7 0.05800335916667669 8 0.04985056502993637 9 0.041428405448149574 10-11 0.03986608586687995 12-13 0.04068316105018763 14-15 0.01814625214796495 16-17 0.01701491727876971 18-19 0.017409986598171223 20-21 0.021387616336691003 22-23 0.025814188483621596 24-25 0.0246559170699217 26-27 0.019277587017160196 28-29 0.018289913718656414 30-31 0.016126011310116307 32-33 0.02340785717453965 34-35 0.023623349530576838 36-37 0.02379394764577295 38-39 0.02132476439951349 40-41 0.025356267227042567 42-43 0.033509061363782885 44-45 0.04317928084095173 46-47 0.0488090186424233 48-49 0.04029707057895433 50-51 0.02743038115390051 52-53 0.040144430160094655 54-55 0.036130885028902014 56-57 0.03826785089293747 58-59 0.04307153466293314 60-61 0.02248303581321338 62-63 0.025221584504519324 64-65 0.02475468439977208 66-67 0.03166839748929856 68-69 0.03842947015996536 70-71 0.0402791128826179 72-73 0.04405022911326871 74-75 0.025832146179958024 76-77 0.01981631790725317 78-79 0.019511037069533815 80-81 0.023668243771417918 82-83 0.01938533319517879 84-85 0.012211233508774042 86-87 0.013971087749744418 88-89 0.018164209844301386 90-91 0.020633393090560843 92-93 0.019394312043347003 94-95 0.02335398408553035 96-97 0.024458382410220948 98-99 0.027493233091078026 100 0.02954041047343132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5568643.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.51274586236678 #Duplication Level Percentage of deduplicated Percentage of total 1 81.22793697073483 58.08832813512508 2 12.543456532286774 17.940340384581372 3 3.2525436415967697 6.977949805433004 4 1.2531700933145744 3.584705376220945 5 0.5799695129280551 2.0737606192972327 6 0.31873738152343684 1.367627121703307 7 0.19553701467456852 0.9788372185975813 8 0.1266131092305646 0.7243560882599562 9 0.09034148993596423 0.581451120955636 >10 0.3865429586485143 4.600278045703542 >50 0.01677519813656585 0.8176345364956132 >100 0.0076529093217543994 0.9864725389535568 >500 4.6284073457451895E-4 0.22689354274788373 >1k 1.7356462208411756E-4 0.2464005285930381 >5k 2.892743701376171E-5 0.17227970801047748 >10k+ 5.785487402752342E-5 0.6326852293218592 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17158 0.30811815374050733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13426 0.24110003101294156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8328 0.14955169508980912 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.591539267286482E-5 0.0 0.0 0.0 0.0 7 3.591539267286482E-5 0.0 0.0 0.0 0.0 8 3.591539267286482E-5 0.0 0.0 0.0 0.0 9 3.591539267286482E-5 0.0 0.0 0.0 0.0 10-11 3.591539267286482E-5 0.0 0.0 2.693654450464862E-5 1.795769633643241E-5 12-13 3.591539267286482E-5 0.0 0.0 8.080963351394586E-5 3.591539267286482E-5 14-15 3.591539267286482E-5 8.978848168216206E-6 0.0 8.978848168216207E-5 3.591539267286482E-5 16-17 7.183078534572965E-5 1.795769633643241E-5 0.0 8.978848168216207E-5 3.591539267286482E-5 18-19 7.183078534572965E-5 1.795769633643241E-5 0.0 9.876732985037828E-5 3.591539267286482E-5 20-21 7.183078534572965E-5 1.795769633643241E-5 0.0 1.705981151961079E-4 3.591539267286482E-5 22-23 8.080963351394586E-5 1.795769633643241E-5 0.0 4.489424084108103E-4 6.285193717751345E-5 24-25 8.978848168216207E-5 1.795769633643241E-5 0.0 0.0010056309948402152 8.978848168216207E-5 26-27 8.978848168216207E-5 1.795769633643241E-5 0.0 0.0019035158116618358 1.0774617801859448E-4 28-29 8.978848168216207E-5 1.795769633643241E-5 0.0 0.00461512795846313 1.0774617801859448E-4 30-31 8.978848168216207E-5 1.795769633643241E-5 0.0 0.016287630577144198 1.1672502618681069E-4 32-33 8.978848168216207E-5 1.795769633643241E-5 0.0 0.04435550995098806 1.346827225232431E-4 34-35 8.978848168216207E-5 1.795769633643241E-5 0.0 0.08599940775517481 1.616192670278917E-4 36-37 8.978848168216207E-5 1.795769633643241E-5 0.0 0.15289182660838557 1.616192670278917E-4 38-39 8.978848168216207E-5 2.693654450464862E-5 0.0 0.24951321174656016 1.616192670278917E-4 40-41 8.978848168216207E-5 3.591539267286482E-5 0.0 0.3532368657857938 1.616192670278917E-4 42-43 8.978848168216207E-5 3.591539267286482E-5 0.0 0.45474274432747797 1.616192670278917E-4 44-45 8.978848168216207E-5 3.591539267286482E-5 0.0 0.5746642404621737 1.616192670278917E-4 46-47 8.978848168216207E-5 3.591539267286482E-5 0.0 0.7169430685357276 1.616192670278917E-4 48-49 8.978848168216207E-5 3.591539267286482E-5 0.0 0.8719359456154758 1.9753465970075653E-4 50-51 8.978848168216207E-5 3.591539267286482E-5 0.0 1.0272789977737844 1.9753465970075653E-4 52-53 8.978848168216207E-5 3.591539267286482E-5 0.0 1.1774502333872003 2.0651350786897275E-4 54-55 8.978848168216207E-5 3.591539267286482E-5 0.0 1.346216663556992 2.1549235603718895E-4 56-57 8.978848168216207E-5 3.591539267286482E-5 0.0 1.5405997475507047 2.1549235603718895E-4 58-59 8.978848168216207E-5 3.591539267286482E-5 0.0 1.7567565383523418 2.2447120420540515E-4 60-61 8.978848168216207E-5 3.591539267286482E-5 0.0 1.9776810975313015 2.3345005237362137E-4 62-63 8.978848168216207E-5 3.591539267286482E-5 0.0 2.1979053065531406 2.514077487100538E-4 64-65 8.978848168216207E-5 3.591539267286482E-5 0.0 2.4267312521201303 2.514077487100538E-4 66-67 8.978848168216207E-5 3.591539267286482E-5 0.0 2.681739518945639 2.6038659687827E-4 68-69 8.978848168216207E-5 3.591539267286482E-5 0.0 2.961771835615966 2.693654450464862E-4 70-71 8.978848168216207E-5 3.591539267286482E-5 0.0 3.256035626632916 2.873231413829186E-4 72-73 1.0774617801859448E-4 3.591539267286482E-5 0.0 3.5516193083305936 3.411962303922158E-4 74-75 1.0774617801859448E-4 3.591539267286482E-5 0.0 3.8470772861539158 3.411962303922158E-4 76-77 1.0774617801859448E-4 3.591539267286482E-5 0.0 4.1733416920423885 3.411962303922158E-4 78-79 1.0774617801859448E-4 3.591539267286482E-5 0.0 4.531831184006588 3.411962303922158E-4 80-81 1.0774617801859448E-4 3.591539267286482E-5 0.0 4.9071829528306985 3.411962303922158E-4 82-83 1.0774617801859448E-4 3.591539267286482E-5 0.0 5.287724495896038 3.411962303922158E-4 84-85 1.0774617801859448E-4 3.591539267286482E-5 0.0 5.662995455086634 3.411962303922158E-4 86-87 1.0774617801859448E-4 3.591539267286482E-5 0.0 6.055945766320448 3.5915392672864827E-4 88 1.0774617801859448E-4 3.591539267286482E-5 0.0 6.375898041946664 3.5915392672864827E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8495 0.0 48.924717 1 GTATCAA 15340 0.0 40.471603 1 ATCAACG 18135 0.0 32.94969 3 CAACGCA 18275 0.0 32.8259 5 TACCTGG 4425 0.0 32.655483 2 TATCAAC 19500 0.0 31.572147 2 AACGCAG 20155 0.0 30.416851 6 GCATCGT 2895 0.0 30.202093 8 TCAACGC 20020 0.0 30.058636 4 GTACTAG 3380 0.0 29.522297 1 GTGGTAT 3605 0.0 29.377056 1 GTACCTG 5145 0.0 28.268587 1 ACGCAGA 22095 0.0 27.065527 7 GTACATG 19600 0.0 26.944355 1 CGCAGAG 22490 0.0 26.524355 8 ACCTGGG 5460 0.0 26.348272 3 TACATGG 19855 0.0 25.83971 2 ACATGGG 19810 0.0 25.156183 3 TGGTATC 4135 0.0 24.245722 2 CTGTGCG 3935 0.0 24.130352 9 >>END_MODULE