FastQCFastQC Report
Mon 6 Jun 2016
ERR1211231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5568643
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153860.2762971158323491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132450.2378496879760473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84000.15084464922603227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86900.049.451291
GTATCAA153500.039.052871
TACCTGG43950.034.3246572
ATCAACG181650.032.0314373
CAACGCA181800.031.995245
TATCAAC194800.030.6390132
GTACCTG51850.029.742421
AACGCAG201050.029.5861366
GTACTAG33850.029.4461421
TCAACGC201650.029.0409644
ACCTGGG55150.027.0150013
GTACATG187000.025.9471261
ACGCAGA225100.025.8197967
CGCAGAG228300.025.3961498
TACATGG190600.025.0759892
ACATGGG185800.024.4608753
GTGGTAT38850.024.4462091
CTAACGC14450.024.394093
CTGTGCG42500.024.3216119
TAACGCC15000.023.4996414
Produced by FastQC (version 0.11.5)