##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211231_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5568643 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.155016042508024 33.0 31.0 34.0 30.0 34.0 2 32.29313766387969 34.0 31.0 34.0 30.0 34.0 3 32.37096488318608 34.0 31.0 34.0 30.0 34.0 4 35.985573505071166 37.0 35.0 37.0 35.0 37.0 5 35.87067495617873 37.0 35.0 37.0 35.0 37.0 6 35.896780059343 37.0 35.0 37.0 35.0 37.0 7 35.83758502744744 37.0 35.0 37.0 35.0 37.0 8 35.822332119333204 37.0 35.0 37.0 35.0 37.0 9 37.43217836014986 39.0 37.0 39.0 34.0 39.0 10-11 37.41293264804369 39.0 37.0 39.0 34.0 39.0 12-13 37.32009306396549 39.0 37.0 39.0 34.0 39.0 14-15 38.55319734089616 40.0 38.0 41.0 34.0 41.0 16-17 38.5048816201721 40.0 38.0 41.0 34.0 41.0 18-19 38.5254736207726 40.0 38.0 41.0 34.0 41.0 20-21 38.48483688036744 40.0 38.0 41.0 34.0 41.0 22-23 38.35989504085646 40.0 38.0 41.0 34.0 41.0 24-25 38.29892758433249 40.0 38.0 41.0 34.0 41.0 26-27 38.16615430366069 40.0 38.0 41.0 33.5 41.0 28-29 38.14198477079604 40.0 38.0 41.0 33.5 41.0 30-31 38.15783683744855 40.0 37.5 41.0 33.5 41.0 32-33 38.031568373120706 40.0 37.0 41.0 33.0 41.0 34-35 37.89218405633114 40.0 37.0 41.0 33.0 41.0 36-37 37.747689697472076 40.0 37.0 41.0 33.0 41.0 38-39 37.65973433743194 40.0 37.0 41.0 33.0 41.0 40-41 37.5641043967085 40.0 37.0 41.0 32.5 41.0 42-43 37.42670781732641 40.0 36.0 41.0 32.0 41.0 44-45 37.29542646206625 40.0 36.0 41.0 31.5 41.0 46-47 37.072958528675656 39.5 36.0 41.0 31.0 41.0 48-49 36.854183236382724 39.0 36.0 41.0 31.0 41.0 50-51 36.66587622873293 39.0 35.0 41.0 30.0 41.0 52-53 36.47104510021562 39.0 35.0 40.5 30.0 41.0 54-55 36.24202548089364 39.0 35.0 40.0 29.5 41.0 56-57 35.99590016813791 38.0 35.0 40.0 29.0 41.0 58-59 35.731346398036294 38.0 34.5 40.0 28.5 41.0 60-61 35.47439061904309 37.5 34.0 40.0 28.0 41.0 62-63 35.178731604809286 37.0 34.0 40.0 28.0 41.0 64-65 34.80568111477069 36.5 34.0 39.5 27.0 41.0 66-67 34.500484498647154 36.0 33.5 39.0 26.5 40.5 68-69 34.17021651414896 35.5 33.0 39.0 26.0 40.0 70-71 33.83980854940782 35.0 33.0 38.0 26.0 40.0 72-73 33.49454552931478 35.0 33.0 37.5 26.0 40.0 74-75 33.150825793644884 35.0 32.0 37.0 26.0 39.0 76-77 32.14223563981386 34.0 31.0 36.0 24.5 38.5 78-79 32.63013556444541 35.0 32.0 36.0 26.0 38.5 80-81 32.54727974122241 35.0 32.0 36.0 25.5 37.5 82-83 32.28758083432535 35.0 32.0 35.0 25.0 37.0 84-85 32.02626052702607 34.0 32.0 35.0 25.0 37.0 86-87 31.781015859698673 34.0 31.0 35.0 24.5 36.0 88-89 31.56058064774488 34.0 31.0 35.0 24.0 36.0 90-91 31.34674614623347 34.0 31.0 35.0 24.0 35.5 92-93 31.16334868297357 34.0 31.0 35.0 23.0 35.0 94-95 30.97102813019258 34.0 30.5 35.0 23.0 35.0 96-97 30.8006759456478 34.0 30.0 35.0 20.5 35.0 98-99 30.62202937053067 34.0 30.0 35.0 20.0 35.0 100 28.84316663862273 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3516323574778788 1101 2 -0.36680870718970837 1101 3 -0.3894381954378403 1101 4 -0.17773432282734802 1101 5 -0.23325073373148086 1101 6 -0.3392564621525693 1101 7 -0.344560082228746 1101 8 -0.3045614509965162 1101 9 -0.5148026636229659 1101 10-11 -0.4836473243375181 1101 12-13 -0.4982552242143825 1101 14-15 -0.7378713893800608 1101 16-17 -0.6742069526850187 1101 18-19 -0.7250046570880215 1101 20-21 -0.7392520083837297 1101 22-23 -0.746723370708331 1101 24-25 -0.7510678556119998 1101 26-27 -0.8755946483962376 1101 28-29 -0.8634727035117606 1101 30-31 -0.7435769928200031 1101 32-33 -0.7637463847868915 1101 34-35 -0.7005171054681583 1101 36-37 -0.6907970010750901 1101 38-39 -0.7337782972400717 1101 40-41 -0.7999863831996876 1101 42-43 -0.8158257575222834 1101 44-45 -0.797344542778653 1101 46-47 -0.7419232010802546 1101 48-49 -0.8544508707110623 1101 50-51 -0.8072820424085307 1101 52-53 -0.7954951523533751 1101 54-55 -0.7353555639577962 1101 56-57 -0.7886682073312059 1101 58-59 -0.8290851926478169 1101 60-61 -0.8214470864071117 1101 62-63 -0.957974617603341 1101 64-65 -0.8606252654276361 1101 66-67 -0.886655545865132 1101 68-69 -0.9392135101652812 1101 70-71 -0.9996731803254661 1101 72-73 -1.0404857557837985 1101 74-75 -1.0325526690915723 1101 76-77 -0.9530270074719169 1101 78-79 -0.9349281685074082 1101 80-81 -0.8683395831762226 1101 82-83 -0.7996202529742433 1101 84-85 -0.7732455196348269 1101 86-87 -0.8807044844701686 1101 88-89 -0.9131504469882827 1101 90-91 -0.8380392430083106 1101 92-93 -0.8926364784940297 1101 94-95 -1.0760939085257526 1101 96-97 -1.0673782221187764 1101 98-99 -1.2076820149158678 1101 100 -0.9754072859471705 1102 1 -0.3758074697765075 1102 2 -0.3838187755061888 1102 3 -0.39200126973488025 1102 4 -0.19216102834668902 1102 5 -0.2964567280538617 1102 6 -0.3495340705299341 1102 7 -0.3818377293638733 1102 8 -0.40319539820528405 1102 9 -0.5113606582807222 1102 10-11 -0.551566499382595 1102 12-13 -0.5731266117342244 1102 14-15 -0.6907001916150364 1102 16-17 -0.761366527077378 1102 18-19 -0.6552862519776923 1102 20-21 -0.7432966375471253 1102 22-23 -0.7347557658666233 1102 24-25 -0.716566569091718 1102 26-27 -0.8547162591686757 1102 28-29 -0.8027811187702909 1102 30-31 -0.7559865943032307 1102 32-33 -0.785348792671968 1102 34-35 -0.7904656565212278 1102 36-37 -0.9067536295614147 1102 38-39 -0.8649663293188468 1102 40-41 -0.8053775676288737 1102 42-43 -0.8540112656009029 1102 44-45 -0.8991860306652697 1102 46-47 -1.0193604115340946 1102 48-49 -0.9275466980824234 1102 50-51 -1.0630715814522276 1102 52-53 -1.0297589487311782 1102 54-55 -0.9315092519167152 1102 56-57 -1.0240239046821529 1102 58-59 -1.127454914770503 1102 60-61 -1.1486756179705608 1102 62-63 -1.06221770230961 1102 64-65 -1.01152102445878 1102 66-67 -1.1142669882627985 1102 68-69 -1.1605766679892469 1102 70-71 -1.2085812556230806 1102 72-73 -1.2235372648448077 1102 74-75 -1.296455729609832 1102 76-77 -1.008030515444549 1102 78-79 -0.9083221142291436 1102 80-81 -0.9515409423870835 1102 82-83 -0.8276778644047766 1102 84-85 -0.8789684783862839 1102 86-87 -0.87816161957576 1102 88-89 -0.8979047069800714 1102 90-91 -0.9389337760287368 1102 92-93 -1.1010934411671442 1102 94-95 -1.125964228229801 1102 96-97 -1.314893375215874 1102 98-99 -1.2814228623503041 1102 100 -1.3656089291179327 1103 1 -0.354171990437397 1103 2 -0.36254995600380724 1103 3 -0.4067909942400121 1103 4 -0.228109770477964 1103 5 -0.27588377794214836 1103 6 -0.3717334609334486 1103 7 -0.3925683137941647 1103 8 -0.40788550691894443 1103 9 -0.5526443990737846 1103 10-11 -0.5109426482582435 1103 12-13 -0.5844538517170079 1103 14-15 -0.6729264050483152 1103 16-17 -0.6964292072823568 1103 18-19 -0.7042018113937374 1103 20-21 -0.7498612211480165 1103 22-23 -0.6985852631602967 1103 24-25 -0.8438630214126945 1103 26-27 -0.8406870106938911 1103 28-29 -0.8840758789659304 1103 30-31 -0.8171859445114151 1103 32-33 -0.8406108024972738 1103 34-35 -0.8685947894099257 1103 36-37 -0.8575787612501315 1103 38-39 -0.8362415702702393 1103 40-41 -0.7620653352096767 1103 42-43 -0.6778010185368757 1103 44-45 -0.7893169988139022 1103 46-47 -0.8236526845211642 1103 48-49 -0.765545107915969 1103 50-51 -0.9306748319799141 1103 52-53 -0.9505821882982914 1103 54-55 -0.880020897288027 1103 56-57 -0.8884862333250538 1103 58-59 -0.9531194367058831 1103 60-61 -0.9914156061472497 1103 62-63 -0.8260360233408832 1103 64-65 -0.7902390679462101 1103 66-67 -0.8587333168311915 1103 68-69 -0.9054583809769454 1103 70-71 -1.020564953965959 1103 72-73 -0.9075000145582948 1103 74-75 -0.938069562293343 1103 76-77 -0.9706534665856523 1103 78-79 -0.8686325594267359 1103 80-81 -0.7058641461705442 1103 82-83 -0.7012784541977126 1103 84-85 -0.8074335363901071 1103 86-87 -0.7287856765786209 1103 88-89 -0.8601703118364519 1103 90-91 -0.9290817046457498 1103 92-93 -1.0249185856007301 1103 94-95 -1.0262835360074014 1103 96-97 -1.0344361484387292 1103 98-99 -1.180921793252569 1103 100 -1.2387975658001906 1104 1 -0.3461411250627684 1104 2 -0.35489891969059784 1104 3 -0.3839638490197359 1104 4 -0.330853260285366 1104 5 -0.26629100120567273 1104 6 -0.3426669644922171 1104 7 -0.397180601341816 1104 8 -0.41864183959959433 1104 9 -0.5979919969262824 1104 10-11 -0.5079333750030202 1104 12-13 -0.5472797295484781 1104 14-15 -0.7212757377119061 1104 16-17 -0.6803546282337223 1104 18-19 -0.7744306739064726 1104 20-21 -0.8096722445625417 1104 22-23 -0.6833316962553511 1104 24-25 -0.8138961944426626 1104 26-27 -0.8648427243306926 1104 28-29 -0.8748993638743201 1104 30-31 -0.8727351695826187 1104 32-33 -0.807243071379041 1104 34-35 -0.831807608408198 1104 36-37 -0.7000094850803009 1104 38-39 -0.6797815693710518 1104 40-41 -0.8196693579870171 1104 42-43 -0.7175541800324439 1104 44-45 -0.8240133952438171 1104 46-47 -0.8379036771152713 1104 48-49 -0.8739790571925141 1104 50-51 -0.8582815058090674 1104 52-53 -0.7988568729238921 1104 54-55 -0.8329153497683066 1104 56-57 -0.8069177485949552 1104 58-59 -0.8905218438919391 1104 60-61 -0.9330574288926599 1104 62-63 -0.9533283886217205 1104 64-65 -0.9308569413065513 1104 66-67 -0.9948122329075701 1104 68-69 -1.008660456238907 1104 70-71 -1.0566270684200845 1104 72-73 -1.0514787743621525 1104 74-75 -0.9672228574267194 1104 76-77 -0.9012303842960776 1104 78-79 -0.7417643236784599 1104 80-81 -0.713554560680322 1104 82-83 -0.771059699788978 1104 84-85 -0.663748272052338 1104 86-87 -0.7449936027584556 1104 88-89 -0.7532405244011997 1104 90-91 -0.7713892747164586 1104 92-93 -0.981812366791285 1104 94-95 -0.9732204277964307 1104 96-97 -0.9958102071990567 1104 98-99 -0.9621865891448351 1104 100 -1.0793176137981817 1105 1 -0.4144112307372865 1105 2 -0.3958080956410903 1105 3 -0.4744594624183769 1105 4 -0.3322467465953167 1105 5 -0.31219585124532756 1105 6 -0.3898402692416809 1105 7 -0.3996873936606775 1105 8 -0.5541303481003723 1105 9 -0.5942715082626293 1105 10-11 -0.5971065168463454 1105 12-13 -0.6479386666156017 1105 14-15 -0.8171657345450427 1105 16-17 -0.8043434807995666 1105 18-19 -0.718037942805644 1105 20-21 -0.8808406648658504 1105 22-23 -0.8908007030207443 1105 24-25 -0.8631488910639789 1105 26-27 -0.9081363418781905 1105 28-29 -0.95364873757552 1105 30-31 -0.8116821023384304 1105 32-33 -0.8329048376283552 1105 34-35 -0.8362618591631801 1105 36-37 -0.8540766512345144 1105 38-39 -0.9262896968813763 1105 40-41 -0.9863047667536406 1105 42-43 -0.9232096581961713 1105 44-45 -0.8735721683096429 1105 46-47 -0.8805290714895762 1105 48-49 -0.9511129450455513 1105 50-51 -1.0441047020000767 1105 52-53 -0.977861625949977 1105 54-55 -0.9383696936125077 1105 56-57 -1.000228808963314 1105 58-59 -1.1053183184571367 1105 60-61 -0.9883378236639615 1105 62-63 -1.0732817778450894 1105 64-65 -1.1181450378418987 1105 66-67 -1.0740066324045259 1105 68-69 -0.9674692747085416 1105 70-71 -1.079346827428516 1105 72-73 -1.0959414830029317 1105 74-75 -1.0518085031601814 1105 76-77 -1.0162125125346932 1105 78-79 -0.8760970313974781 1105 80-81 -0.8713689672411071 1105 82-83 -0.7936920735732329 1105 84-85 -0.852789092588182 1105 86-87 -0.8456921826002564 1105 88-89 -0.8627777888375583 1105 90-91 -0.9068951233661657 1105 92-93 -1.0224301826078737 1105 94-95 -0.9656947461232299 1105 96-97 -0.9601299987887195 1105 98-99 -1.0133398573522214 1105 100 -1.0145219263844858 1106 1 -0.3949765360043962 1106 2 -0.3716744219117665 1106 3 -0.37421221884049416 1106 4 -0.2926230833915042 1106 5 -0.31056710468133986 1106 6 -0.3771628029466285 1106 7 -0.44474199336252696 1106 8 -0.4315678465503723 1106 9 -0.58860243155533 1106 10-11 -0.5831766129352474 1106 12-13 -0.648178782545628 1106 14-15 -0.7692435360954519 1106 16-17 -0.7271606508742323 1106 18-19 -0.7460385912936474 1106 20-21 -0.7580948352454655 1106 22-23 -0.7121571606414747 1106 24-25 -0.7479748325109199 1106 26-27 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0.918524825621617 2311 34-35 0.9341308838972253 2311 36-37 0.9790117681898138 2311 38-39 1.0079517863045524 2311 40-41 0.9625665511086581 2311 42-43 0.9789980913955105 2311 44-45 1.0299228515170995 2311 46-47 0.6333407157164146 2311 48-49 0.8942810966771191 2311 50-51 1.0276305834295698 2311 52-53 0.9716801606111787 2311 54-55 1.0054069669745545 2311 56-57 1.0959006939052074 2311 58-59 1.1316261058880812 2311 60-61 1.2084689475240324 2311 62-63 1.1854613787387507 2311 64-65 1.204458897637295 2311 66-67 1.2565984273260824 2311 68-69 1.295668596529012 2311 70-71 1.2982784691537574 2311 72-73 1.3068582370496884 2311 74-75 1.365865772643886 2311 76-77 1.2568593166460218 2311 78-79 0.9582955386464036 2311 80-81 0.9091769562602607 2311 82-83 0.9595975469809375 2311 84-85 1.0703000931284734 2311 86-87 1.11353399031427 2311 88-89 1.1093609437149468 2311 90-91 1.1533747319784347 2311 92-93 1.2560725172004759 2311 94-95 1.1895743234973217 2311 96-97 1.252693097778085 2311 98-99 1.3362215330016909 2311 100 1.5087305659271522 2312 1 0.21576983024006324 2312 2 0.3365734987840767 2312 3 0.4411449377106038 2312 4 0.16760467692368763 2312 5 0.3189828178497649 2312 6 0.4025372308138415 2312 7 0.2686572802979299 2312 8 0.34975696418190694 2312 9 0.5778359775233568 2312 10-11 0.5966904512024414 2312 12-13 0.6705664389736867 2312 14-15 0.8480866609871072 2312 16-17 0.8522173159443653 2312 18-19 0.8023632818243911 2312 20-21 0.7926871825843449 2312 22-23 0.9130037957541788 2312 24-25 0.8897676541976338 2312 26-27 0.902297794322628 2312 28-29 0.9644421618547341 2312 30-31 0.9635221095828612 2312 32-33 0.8266875092096413 2312 34-35 0.9197477018771565 2312 36-37 0.9728315272024872 2312 38-39 0.7978056360415735 2312 40-41 0.8435799995397488 2312 42-43 0.9864540127262202 2312 44-45 0.7247564841640539 2312 46-47 0.6886473564675484 2312 48-49 0.9822058712964292 2312 50-51 0.7572133564617474 2312 52-53 0.9256708871704049 2312 54-55 0.8076557491107508 2312 56-57 0.8489108646379719 2312 58-59 0.8970641256927863 2312 60-61 0.9920748706279952 2312 62-63 1.0590085825098754 2312 64-65 0.9661184137565471 2312 66-67 1.0223214654033086 2312 68-69 1.0857216806218588 2312 70-71 1.0221946310725443 2312 72-73 0.9887544256977634 2312 74-75 1.0277316468060604 2312 76-77 0.954932268440281 2312 78-79 0.8917283637046225 2312 80-81 0.857293614081108 2312 82-83 0.8809836838799896 2312 84-85 0.8823835866110556 2312 86-87 0.9646309378438467 2312 88-89 1.0249493573198478 2312 90-91 1.105288015351885 2312 92-93 1.134191858615015 2312 94-95 1.1323170218419243 2312 96-97 0.7874358318001136 2312 98-99 0.5752723799170028 2312 100 0.7745390221802708 2313 1 0.04486089548440475 2313 2 0.26063126097656664 2313 3 0.299636326234733 2313 4 0.2322277606891774 2313 5 0.3353690347928051 2313 6 0.23436119203845607 2313 7 0.23593347247276597 2313 8 0.3728622309271685 2313 9 0.5523523024937376 2313 10-11 0.3851415668619822 2313 12-13 0.42099718328324087 2313 14-15 0.502618728180714 2313 16-17 0.7500541939767871 2313 18-19 0.7050456911473404 2313 20-21 0.647783261045646 2313 22-23 0.6565500201377432 2313 24-25 0.5699056227576378 2313 26-27 0.751841512051314 2313 28-29 0.648352721639526 2313 30-31 0.7970879440030529 2313 32-33 0.9209174494167556 2313 34-35 0.928756654037052 2313 36-37 0.9730090494439239 2313 38-39 0.932309206414736 2313 40-41 0.9314762329177455 2313 42-43 1.0467823666983378 2313 44-45 0.8612544074183077 2313 46-47 0.9001808015696753 2313 48-49 1.0565276038652485 2313 50-51 0.9721410096525176 2313 52-53 0.9999857990990009 2313 54-55 0.9761173383969464 2313 56-57 0.6558397790440011 2313 58-59 0.7093935139894612 2313 60-61 0.621185193741205 2313 62-63 0.9891522697359179 2313 64-65 1.0859885093276276 2313 66-67 1.0672918951440096 2313 68-69 1.1728867483278478 2313 70-71 1.1168135101685337 2313 72-73 1.1408304940246126 2313 74-75 1.1492957318944335 2313 76-77 1.123957929312759 2313 78-79 1.045868696474848 2313 80-81 0.9373329223122866 2313 82-83 0.9058540232773709 2313 84-85 0.9606835387876913 2313 86-87 0.957574985533757 2313 88-89 0.9325019357561644 2313 90-91 0.8692708033966845 2313 92-93 0.9981493638920789 2313 94-95 1.1724106474246874 2313 96-97 1.2753924371238625 2313 98-99 1.252894274617546 2313 100 1.337289051423184 2314 1 -0.3187198285021182 2314 2 0.05872001100850355 2314 3 0.2880552701108172 2314 4 0.21244957189644254 2314 5 0.3357041833239123 2314 6 0.42040713284499986 2314 7 0.4461169966330232 2314 8 0.48885592396651845 2314 9 0.4729733811738015 2314 10-11 0.5823230297629678 2314 12-13 0.4444527215830618 2314 14-15 0.7331140965064833 2314 16-17 0.8356657328313943 2314 18-19 0.8355897395007119 2314 20-21 0.8785237860052248 2314 22-23 0.9212550155206713 2314 24-25 0.7816331980086559 2314 26-27 0.7544130903974136 2314 28-29 0.8725338524031088 2314 30-31 0.8526011282152126 2314 32-33 0.8704360098748865 2314 34-35 0.9636657542366933 2314 36-37 0.8913640659960436 2314 38-39 0.6103709839702773 2314 40-41 0.6620964493180352 2314 42-43 0.8802317504192203 2314 44-45 0.9480734116995961 2314 46-47 0.9061231410578898 2314 48-49 0.9386654782954196 2314 50-51 0.9289263302745994 2314 52-53 0.9554872809427621 2314 54-55 0.8735563902724266 2314 56-57 0.8435093151100972 2314 58-59 0.998563644982994 2314 60-61 1.1038628826854122 2314 62-63 1.011042245573833 2314 64-65 0.9868735779267794 2314 66-67 0.7151984258411659 2314 68-69 0.6680557688510262 2314 70-71 0.8015792164349804 2314 72-73 1.021991528650851 2314 74-75 1.0557041586946383 2314 76-77 0.909540543015062 2314 78-79 0.8301390481025024 2314 80-81 0.8796045687283041 2314 82-83 0.8857896169090864 2314 84-85 0.8690809119823797 2314 86-87 0.9351711320100016 2314 88-89 0.9104533506249766 2314 90-91 1.0201499865941877 2314 92-93 0.937914764482624 2314 94-95 0.9869165656750916 2314 96-97 0.9398461623549856 2314 98-99 1.0664378612634309 2314 100 1.0913520316080856 2315 1 0.38634138239128646 2315 2 0.4218954286926433 2315 3 0.40481729855388693 2315 4 0.2716673478523717 2315 5 0.33062899403404344 2315 6 0.39112882187417597 2315 7 0.3374214465469123 2315 8 0.2421832650955693 2315 9 0.4720788076882485 2315 10-11 0.5335033484440714 2315 12-13 0.2967320011667951 2315 14-15 0.5855795828142405 2315 16-17 0.7292397630335046 2315 18-19 0.7298164203754496 2315 20-21 0.442790275685347 2315 22-23 0.772272947806421 2315 24-25 0.8412974378033269 2315 26-27 0.7635313827420802 2315 28-29 0.7595261645045 2315 30-31 0.816051450705821 2315 32-33 0.7563379192755662 2315 34-35 0.7694656696202173 2315 36-37 0.7608300034746449 2315 38-39 0.7990342191637083 2315 40-41 0.8020563069622213 2315 42-43 0.9479461970151632 2315 44-45 0.9101025679574448 2315 46-47 0.8664024632131913 2315 48-49 0.8072723368823205 2315 50-51 0.8620051631640777 2315 52-53 0.93108194431138 2315 54-55 0.7505783093636254 2315 56-57 0.6992162094220191 2315 58-59 0.7331187677032176 2315 60-61 0.8585247876270472 2315 62-63 0.9556093344158683 2315 64-65 1.0018564329956021 2315 66-67 0.9910207154366759 2315 68-69 1.0150427002572542 2315 70-71 0.9215937566005934 2315 72-73 0.9540329428871388 2315 74-75 0.3480198589135455 2315 76-77 0.39290235614255664 2315 78-79 0.7240472212292204 2315 80-81 0.7357886157132683 2315 82-83 0.6915836841879681 2315 84-85 0.68293552173715 2315 86-87 0.7708384259855556 2315 88-89 0.7407794268956707 2315 90-91 0.844497072592258 2315 92-93 0.8371584279021498 2315 94-95 0.8632100391464199 2315 96-97 1.0304822166991308 2315 98-99 1.041819930118777 2315 100 0.9683898711492631 2316 1 0.2722929191710364 2316 2 0.09301774697606646 2316 3 0.2501198792583068 2316 4 0.027707166254920423 2316 5 0.309489014592117 2316 6 0.3421177906420638 2316 7 0.4369919894954677 2316 8 0.4370058697210979 2316 9 0.6282619525207949 2316 10-11 0.5994669014582783 2316 12-13 0.3695103573393581 2316 14-15 0.48068911220910593 2316 16-17 0.782150308575126 2316 18-19 0.7392689706432876 2316 20-21 0.7279956069382436 2316 22-23 0.7532549941080049 2316 24-25 0.4254402984483292 2316 26-27 0.7560597243264979 2316 28-29 0.9300720026343754 2316 30-31 0.5902881321551448 2316 32-33 0.6320341798057925 2316 34-35 0.787120116710291 2316 36-37 0.782810420132968 2316 38-39 0.6442211952419044 2316 40-41 0.7344380669734889 2316 42-43 0.7514920284983617 2316 44-45 0.5976394483580805 2316 46-47 0.7346920969763602 2316 48-49 0.8124842129496699 2316 50-51 0.8052009191514315 2316 52-53 0.8920001570919069 2316 54-55 0.8229397055239573 2316 56-57 0.9574730491933536 2316 58-59 0.7844139775930543 2316 60-61 0.8656195063364152 2316 62-63 0.8450523380765489 2316 64-65 0.8873944409999268 2316 66-67 0.9043534463973231 2316 68-69 0.8984041172208066 2316 70-71 0.8771950089890836 2316 72-73 0.927885364658394 2316 74-75 0.9521096860171951 2316 76-77 0.7787903503010796 2316 78-79 0.5185289227670324 2316 80-81 0.5267939780242585 2316 82-83 0.4427041232757887 2316 84-85 0.5324898796064303 2316 86-87 0.5926841223600299 2316 88-89 0.5897442702275555 2316 90-91 0.526942083893335 2316 92-93 0.32306026354046935 2316 94-95 0.3236052041151076 2316 96-97 0.6041601719478606 2316 98-99 0.8015130554049286 2316 100 0.44808167530134213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 4.0 12 22.0 13 119.0 14 449.0 15 1395.0 16 3362.0 17 6760.0 18 11512.0 19 16788.0 20 23584.0 21 32948.0 22 39686.0 23 42553.0 24 43232.0 25 46796.0 26 54555.0 27 67259.0 28 83355.0 29 102711.0 30 127149.0 31 158674.0 32 201957.0 33 263992.0 34 373948.0 35 524141.0 36 817517.0 37 1247860.0 38 1101591.0 39 174706.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40399354100149 20.222673039430216 13.706364211916666 23.66696920765163 2 15.465006465668566 20.23369068550453 40.68560329688938 23.615699551937517 3 18.97221639095916 25.485436936790524 29.64081195364831 25.901534718601997 4 12.53843631970611 16.5227716724975 38.43058917576471 32.50820283203169 5 13.144494987378433 37.662694484096036 34.749758603667 14.443051924858533 6 30.942780853432332 37.174981409294865 17.817913628149622 14.064324109123175 7 27.56825278018662 32.141088937778086 22.1336023177096 18.15705596432569 8 26.052361410131695 35.88870753610889 19.77494337489403 18.28398767886539 9 26.09115907247191 16.011305220663548 20.87703282677032 37.02050288009423 10-11 25.399984538363757 26.366552296650074 27.607694292025414 20.62576887296075 12-13 25.53157255111995 23.855005955221582 28.172108372911353 22.44131312074711 14-15 22.73551368773251 25.398266289985553 25.95896912637134 25.907250895910593 16-17 21.997174889124455 28.104115715334704 26.978120834366653 22.920588561174192 18-19 21.61665777461403 27.550616909721093 29.11211941580741 21.72060589985747 20-21 22.653691026893462 26.804659665555686 28.878729815538446 21.66291949201241 22-23 22.643083781811836 26.621826897504477 28.610129972418775 22.12495934826492 24-25 22.209653765761665 26.60288299719796 29.143910033635834 22.043553203404546 26-27 22.21733570938773 26.818667943584902 28.634280818993517 22.32971552803385 28-29 21.840041920514082 27.168108013563106 28.84212717158283 22.149722894339977 30-31 22.484504608548374 26.834770267560774 28.833177202413463 21.847547921477386 32-33 22.481979307320028 26.9835064907541 28.433414766353327 22.101099435572543 34-35 22.47992477347169 27.006485324270702 28.762983446750916 21.750606455506688 36-37 22.403932464278537 26.769008051352184 28.574073032865204 22.252986451504082 38-39 22.36072906275997 26.927721755661466 28.800860079162703 21.910689102415862 40-41 22.35204507583723 26.462713683636252 29.017058415884435 22.168182824642084 42-43 22.30439071378123 26.551784017602863 28.62343024929431 22.520395019321597 44-45 22.257818409874115 27.096780682225543 28.525567114438687 22.119833793461652 46-47 22.245963669568624 26.871046671835842 28.138554380247644 22.74443527834789 48-49 22.54290841890983 26.87312835320583 28.20927012385923 22.37469310402511 50-51 22.419039501266518 26.95354761401891 28.062352101930593 22.565060782783984 52-53 22.181880844689296 27.258797110758366 27.845808454346244 22.713513590206098 54-55 22.14143698819094 27.15689525724016 27.909249467789966 22.792418286778933 56-57 22.617391120866177 26.962634801187193 27.670126382356123 22.74984769559051 58-59 22.084207015745726 27.18383936198518 28.078975651951687 22.652977970317412 60-61 22.3093601045044 27.466400449107443 27.706461378879094 22.517778067509067 62-63 22.42438967932243 27.148162045595743 27.56669869464657 22.860749580435257 64-65 21.993897570075998 27.113291961840503 27.711498957628798 23.181311510454698 66-67 22.18495515598576 26.978049715955414 27.648865892557467 23.188129235501354 68-69 21.970597711058975 27.02348722373587 27.78163402710483 23.224281038100322 70-71 21.99782257146602 26.771321223352263 27.817660168967663 23.413196036214057 72-73 22.20919392413596 26.968936357287852 27.74334007235601 23.078529646220176 74-75 22.343682649125924 27.165842434557014 27.445703210153454 23.044771706163612 76-77 22.23070293209017 27.016784260920208 27.35105576155557 23.40145704543405 78-79 22.10302491163923 27.06227362015044 27.398762060196496 23.43593940801383 80-81 21.888976034701894 27.288133538712124 27.5428307703635 23.280059656222488 82-83 22.38566450570027 26.86470704794097 27.962373423400845 22.78725502295791 84-85 22.53754180270006 27.03004404274804 27.332744731193344 23.099669423358556 86-87 21.863430518809576 27.360613257912895 27.57360693215889 23.202349291118637 88-89 22.190547361893064 26.773696086608794 27.55421428341022 23.481542268087924 90-91 22.210314366784665 26.745054292334718 27.570529189687104 23.474102151193513 92-93 22.306351305662535 26.942406339700415 27.430617309500533 23.32062504513652 94-95 21.69688531747678 26.883706461965414 27.79452251370754 23.624885706850264 96-97 21.85388903510036 26.8442018142135 27.58752776932984 23.714381381356304 98-99 21.5898451655374 27.075882402864178 27.41138027238041 23.92289215921802 100 21.843323386273344 26.762321791821662 27.26059692419564 24.133757897709348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1003.0 1 709.0 2 791.0 3 1201.5 4 933.0 5 682.5 6 1026.0 7 1884.0 8 2940.5 9 3515.5 10 3226.0 11 2884.5 12 3016.0 13 3402.0 14 4003.0 15 4654.5 16 5276.5 17 5925.5 18 6707.0 19 7453.5 20 8240.5 21 9244.5 22 10575.5 23 12464.0 24 14774.0 25 17831.5 26 22075.0 27 26938.0 28 33288.0 29 42839.5 30 54595.0 31 66493.5 32 81535.5 33 97270.0 34 110877.5 35 125140.5 36 139428.0 37 157196.0 38 168760.5 39 170057.5 40 169006.0 41 167083.5 42 169501.5 43 177766.0 44 188665.5 45 206354.0 46 222642.5 47 232665.5 48 241236.5 49 241129.5 50 234636.5 51 231931.0 52 226850.5 53 216002.5 54 203916.0 55 182898.5 56 161390.5 57 137936.5 58 114171.5 59 100264.0 60 81585.0 61 60418.0 62 46100.0 63 36335.0 64 26555.0 65 16986.0 66 11609.5 67 8603.5 68 6391.0 69 4720.5 70 3559.0 71 2681.5 72 2011.5 73 1500.0 74 1131.0 75 778.0 76 492.0 77 279.5 78 166.5 79 105.5 80 73.0 81 57.5 82 31.5 83 15.0 84 13.5 85 11.0 86 6.5 87 5.0 88 6.0 89 4.5 90 2.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044086144505941574 2 0.0 3 0.0 4 0.03534074639009899 5 0.0 6 0.0 7 7.542232461301613E-4 8 0.0 9 0.010702787016513718 10-11 3.8609047123329686E-4 12-13 8.978848168216207E-6 14-15 1.257038743550269E-4 16-17 2.1549235603718895E-4 18-19 0.0 20-21 5.387308900929724E-5 22-23 0.0 24-25 5.117943455883238E-4 26-27 4.938366492518914E-4 28-29 1.616192670278917E-4 30-31 0.0023793947645772947 32-33 0.015748899687051225 34-35 0.026182321258518458 36-37 0.031461883981429586 38-39 0.02242018387603587 40-41 0.0012929541362231337 42-43 7.183078534572966E-5 44-45 0.011331306388288852 46-47 0.018388681048506793 48-49 0.0033131949740717805 50-51 0.011115814032251663 52-53 0.01090930052438269 54-55 0.010550146597654042 56-57 4.5792125657902655E-4 58-59 0.008915996231038692 60-61 0.004758789529154589 62-63 0.0013378483770642147 64-65 0.036059054243556286 66-67 0.06119085026639345 68-69 0.06037377508308577 70-71 0.05214017131283151 72-73 0.04586395644324838 74-75 0.03495465591886569 76-77 0.03162350324845748 78-79 0.015156295707948955 80-81 0.0012660175917184851 82-83 0.03864496251600255 84-85 0.05232872712436405 86-87 0.057814803355144155 88-89 0.07079821780638479 90-91 0.049491411103207726 92-93 0.03945305885114201 94-95 0.013001372147577068 96-97 0.004031502827529077 98-99 0.02236631078702657 100 0.03302420356269921 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5568643.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.31849016201983 #Duplication Level Percentage of deduplicated Percentage of total 1 80.14709540669196 56.358227398699334 2 13.107743482465859 18.4343346223611 3 3.5520651082297996 7.493275661037332 4 1.3512730289537482 3.800779167707476 5 0.6205940910262354 2.1819619742217986 6 0.34105721767045116 1.4389577163267286 7 0.20419501730851158 1.005107972101943 8 0.14766825909671893 0.8307047219628182 9 0.10424638057739267 0.6597403278351806 >10 0.3967346324538946 4.668308194084478 >50 0.018609601586012152 0.8829273965958756 >100 0.008066983341904603 1.0285781576312805 >500 3.253958308614789E-4 0.156549885453568 >1k 2.3665073935693232E-4 0.2902592939687364 >5k 2.9581342418476162E-5 0.1747296882099683 >10k+ 5.9162684836952325E-5 0.5955578218023336 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15386 0.2762971158323491 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13245 0.2378496879760473 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8400 0.15084464922603227 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.591539267286482E-5 0.0 0.0 0.0 3.591539267286482E-5 7 3.591539267286482E-5 1.795769633643241E-5 0.0 0.0 3.591539267286482E-5 8 3.591539267286482E-5 1.795769633643241E-5 0.0 0.0 3.591539267286482E-5 9 3.591539267286482E-5 1.795769633643241E-5 0.0 0.0 3.591539267286482E-5 10-11 3.591539267286482E-5 1.795769633643241E-5 0.0 8.978848168216206E-6 3.591539267286482E-5 12-13 3.591539267286482E-5 1.795769633643241E-5 0.0 6.285193717751344E-5 3.591539267286482E-5 14-15 3.591539267286482E-5 1.795769633643241E-5 0.0 7.183078534572965E-5 3.591539267286482E-5 16-17 8.978848168216207E-5 1.795769633643241E-5 0.0 7.183078534572965E-5 4.489424084108103E-5 18-19 8.978848168216207E-5 1.795769633643241E-5 0.0 8.080963351394586E-5 5.387308900929724E-5 20-21 8.978848168216207E-5 1.795769633643241E-5 0.0 1.5264041885967552E-4 6.285193717751344E-5 22-23 8.978848168216207E-5 1.795769633643241E-5 0.0 4.309847120743779E-4 8.978848168216205E-5 24-25 8.978848168216207E-5 1.795769633643241E-5 0.0 9.876732985037828E-4 1.257038743550269E-4 26-27 8.978848168216207E-5 1.795769633643241E-5 0.0 0.0018586215708207548 1.257038743550269E-4 28-29 8.978848168216207E-5 1.795769633643241E-5 0.0 0.004516360628612752 1.257038743550269E-4 30-31 8.978848168216207E-5 1.795769633643241E-5 0.0 0.016269672880807766 1.7957696336432413E-4 32-33 8.978848168216207E-5 1.795769633643241E-5 0.0 0.04437346764732449 1.7957696336432413E-4 34-35 1.1672502618681069E-4 1.795769633643241E-5 0.0 0.08607123854052057 1.9753465970075653E-4 36-37 1.257038743550269E-4 1.795769633643241E-5 0.0 0.1533048536241235 1.9753465970075653E-4 38-39 1.257038743550269E-4 1.795769633643241E-5 0.0 0.2509408486053065 1.9753465970075653E-4 40-41 1.257038743550269E-4 1.795769633643241E-5 0.0 0.3556791124875486 1.9753465970075653E-4 42-43 1.257038743550269E-4 1.795769633643241E-5 0.0 0.45740048338526995 2.0651350786897275E-4 44-45 1.257038743550269E-4 1.795769633643241E-5 0.0 0.5781390547032733 2.2447120420540515E-4 46-47 1.257038743550269E-4 1.795769633643241E-5 0.0 0.7215402387978543 2.693654450464862E-4 48-49 1.257038743550269E-4 1.795769633643241E-5 0.0 0.8772244871865551 3.0528083771935103E-4 50-51 1.257038743550269E-4 1.795769633643241E-5 0.0 1.0334295087690124 3.0528083771935103E-4 52-53 1.257038743550269E-4 1.795769633643241E-5 0.0 1.1838072578902974 3.1425968588756723E-4 54-55 1.257038743550269E-4 1.795769633643241E-5 0.0 1.352932841986818 3.232385340557834E-4 56-57 1.257038743550269E-4 1.795769633643241E-5 0.0 1.5472261374988485 3.232385340557834E-4 58-59 1.257038743550269E-4 1.795769633643241E-5 0.0 1.7634278225413265 3.232385340557834E-4 60-61 1.436615706914593E-4 1.795769633643241E-5 0.0 1.9842266778459312 3.232385340557834E-4 62-63 1.8855581153254033E-4 1.795769633643241E-5 0.0 2.204648421527471 3.232385340557834E-4 64-65 2.1549235603718895E-4 1.795769633643241E-5 0.0 2.4333755997646103 3.232385340557834E-4 66-67 2.1549235603718895E-4 1.795769633643241E-5 0.0 2.688285099260269 3.591539267286482E-4 68-69 2.1549235603718895E-4 1.795769633643241E-5 0.0 2.968784316035343 3.9506931940151306E-4 70-71 2.2447120420540515E-4 1.795769633643241E-5 0.0 3.2637843726020863 4.309847120743779E-4 72-73 2.3345005237362137E-4 1.795769633643241E-5 0.0 3.5608854796401923 5.566885864294048E-4 74-75 2.3345005237362137E-4 1.795769633643241E-5 0.0 3.8575825385107287 5.566885864294048E-4 76-77 2.3345005237362137E-4 1.795769633643241E-5 0.0 4.185139898535424 5.566885864294048E-4 78-79 2.3345005237362137E-4 1.795769633643241E-5 0.0 4.544652979190801 5.566885864294048E-4 80-81 2.3345005237362137E-4 1.795769633643241E-5 0.0 4.920902632831734 5.566885864294048E-4 82-83 2.3345005237362137E-4 1.795769633643241E-5 0.0 5.302683256944286 5.926039791022697E-4 84-85 2.3345005237362137E-4 1.795769633643241E-5 0.0 5.67877129131819 5.926039791022697E-4 86-87 2.3345005237362137E-4 1.795769633643241E-5 0.0 6.072996598991891 6.015828272704859E-4 88 2.3345005237362137E-4 1.795769633643241E-5 0.0 6.393478626660032 6.105616754387021E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8690 0.0 49.45129 1 GTATCAA 15350 0.0 39.05287 1 TACCTGG 4395 0.0 34.324657 2 ATCAACG 18165 0.0 32.031437 3 CAACGCA 18180 0.0 31.99524 5 TATCAAC 19480 0.0 30.639013 2 GTACCTG 5185 0.0 29.74242 1 AACGCAG 20105 0.0 29.586136 6 GTACTAG 3385 0.0 29.446142 1 TCAACGC 20165 0.0 29.040964 4 ACCTGGG 5515 0.0 27.015001 3 GTACATG 18700 0.0 25.947126 1 ACGCAGA 22510 0.0 25.819796 7 CGCAGAG 22830 0.0 25.396149 8 TACATGG 19060 0.0 25.075989 2 ACATGGG 18580 0.0 24.460875 3 GTGGTAT 3885 0.0 24.446209 1 CTAACGC 1445 0.0 24.39409 3 CTGTGCG 4250 0.0 24.321611 9 TAACGCC 1500 0.0 23.499641 4 >>END_MODULE