FastQCFastQC Report
Mon 6 Jun 2016
ERR1211230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5595535
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214970.38418131599570016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168870.3017941984099823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.19293954912264868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102650.050.9332161
TACCTGG49500.047.3449862
GTATCAA187750.041.407221
GTACCTG59400.040.4837231
ACCTGGG58900.039.0266843
ATCAACG216750.034.352963
CAACGCA218000.033.961915
TATCAAC230450.033.224672
GTACTAG35950.032.071051
AACGCAG240150.031.3191056
TCAACGC240050.031.0968824
GCATCGT32300.030.1239978
GTGGTAT38250.030.0195641
GTACATG238950.029.8761441
TACATGG240550.029.1494772
CCTGGGG52000.028.2949624
ACGCAGA263200.028.0940787
ACATGGG240700.028.0639483
CGCAGAG265200.027.7209438
CTGTGCG47550.027.4806779
Produced by FastQC (version 0.11.5)