##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211230_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5595535 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41553899671792 31.0 31.0 34.0 28.0 34.0 2 31.423024250585513 31.0 31.0 34.0 28.0 34.0 3 31.49023480328512 31.0 31.0 34.0 28.0 34.0 4 35.086545611813705 37.0 35.0 37.0 32.0 37.0 5 35.153085987309524 37.0 35.0 37.0 32.0 37.0 6 35.21321678802831 37.0 35.0 37.0 32.0 37.0 7 35.13392034899255 37.0 35.0 37.0 32.0 37.0 8 35.13861284041651 37.0 35.0 37.0 32.0 37.0 9 36.70263200927168 39.0 37.0 39.0 32.0 39.0 10-11 36.648484193200474 39.0 37.0 39.0 32.0 39.0 12-13 36.54056073279856 39.0 37.0 39.0 32.0 39.0 14-15 37.71985556340904 40.0 37.0 41.0 32.0 41.0 16-17 37.70101884806368 40.0 37.0 41.0 32.0 41.0 18-19 37.73459097298114 40.0 37.0 41.0 32.0 41.0 20-21 37.705234173318544 40.0 37.0 41.0 32.0 41.0 22-23 37.591500633987636 40.0 37.0 41.0 32.0 41.0 24-25 37.50171234743416 40.0 37.0 41.0 31.5 41.0 26-27 37.35655339480496 40.0 37.0 41.0 31.0 41.0 28-29 37.236273653904405 40.0 36.0 41.0 31.0 41.0 30-31 37.07733844931718 39.5 36.0 41.0 30.5 41.0 32-33 36.87537625982145 39.0 36.0 41.0 30.0 41.0 34-35 36.71795270693508 39.0 35.0 41.0 30.0 41.0 36-37 36.604287436322 39.0 35.0 41.0 30.0 41.0 38-39 36.46267398917172 39.0 35.0 41.0 29.5 41.0 40-41 36.29809526702987 39.0 35.0 40.5 29.0 41.0 42-43 36.09139295885022 39.0 35.0 40.0 28.0 41.0 44-45 35.86941096785205 38.5 35.0 40.0 27.0 41.0 46-47 35.62479396518832 38.0 34.5 40.0 26.5 41.0 48-49 35.413673134025615 38.0 34.0 40.0 26.0 41.0 50-51 34.74132544966656 37.5 33.5 39.5 24.5 40.5 52-53 34.855851942665 38.0 33.5 40.0 25.5 40.5 54-55 35.34666845976301 38.0 34.0 40.0 26.0 41.0 56-57 35.31768856775983 38.0 34.0 40.0 26.0 41.0 58-59 35.123583089016506 38.0 34.0 40.0 26.0 41.0 60-61 34.913956663661295 37.5 34.0 40.0 26.0 41.0 62-63 34.62720168848912 37.0 33.5 40.0 25.0 41.0 64-65 34.39575482952033 37.0 33.0 40.0 25.0 41.0 66-67 34.23143229378424 36.0 33.0 40.0 25.0 41.0 68-69 33.94134707405101 36.0 33.0 39.0 24.0 41.0 70-71 33.63646264387587 35.0 33.0 39.0 24.0 41.0 72-73 33.29780253362726 35.0 32.0 38.5 23.5 40.0 74-75 32.962646288513966 35.0 32.0 37.5 23.0 40.0 76-77 32.61696745708855 35.0 32.0 37.0 22.5 39.0 78-79 32.26817149745288 35.0 31.5 36.5 22.0 39.0 80-81 31.958055306597135 35.0 31.0 36.0 21.0 38.5 82-83 31.65001246529599 35.0 31.0 36.0 20.0 37.0 84-85 31.387150826507206 35.0 31.0 35.0 20.0 37.0 86-87 31.148936518134548 34.0 31.0 35.0 20.0 36.5 88-89 30.923534568186955 34.0 30.0 35.0 19.0 36.0 90-91 30.689388414155214 34.0 30.0 35.0 18.5 36.0 92-93 30.494953476298512 34.0 30.0 35.0 18.0 36.0 94-95 30.31839672167183 34.0 29.0 35.0 18.0 35.0 96-97 30.13934494199393 34.0 29.0 35.0 17.5 35.0 98-99 29.907386603783195 34.0 29.0 35.0 17.0 35.0 100 28.791315039580667 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.37208103056345365 1101 2 -0.2894544355378841 1101 3 -0.35722131078598096 1101 4 -0.2150998531950492 1101 5 -0.17168799951502223 1101 6 -0.12778846154922974 1101 7 -0.29874145680130937 1101 8 -0.16531134076341658 1101 9 -0.33685822264941834 1101 10-11 -0.37337636331474044 1101 12-13 -0.36323363941520626 1101 14-15 -0.5185439989787923 1101 16-17 -0.5489146180681885 1101 18-19 -0.5122745683710832 1101 20-21 -0.6172356629399403 1101 22-23 -0.6493161316143059 1101 24-25 -0.7137784443420472 1101 26-27 -0.7781169805542163 1101 28-29 -0.7305443422799982 1101 30-31 -0.7720257299840512 1101 32-33 -0.812695738246866 1101 34-35 -0.7468460814691156 1101 36-37 -0.8621968750545221 1101 38-39 -0.8511375866389344 1101 40-41 -0.7811051282045653 1101 42-43 -0.9680904830135191 1101 44-45 -0.8521679112609988 1101 46-47 -0.9486955721955042 1101 48-49 -0.9389997698732913 1101 50-51 -1.0701678818633056 1101 52-53 -0.9168639775691432 1101 54-55 -0.7503747632433289 1101 56-57 -0.7361757302181573 1101 58-59 -0.7473651647980191 1101 60-61 -0.6957114973647336 1101 62-63 -0.8046339726942406 1101 64-65 -0.7540758318884784 1101 66-67 -0.7647858657119784 1101 68-69 -0.8948235844179777 1101 70-71 -0.8269645970273771 1101 72-73 -0.8539399313757414 1101 74-75 -0.91003021129697 1101 76-77 -0.9647141059446014 1101 78-79 -0.9284869945507594 1101 80-81 -0.9310103420569895 1101 82-83 -0.9673353120885082 1101 84-85 -0.9726227916780559 1101 86-87 -1.08815036914514 1101 88-89 -1.2275268611611843 1101 90-91 -1.2984883397459157 1101 92-93 -1.237806875767447 1101 94-95 -1.3924407457576713 1101 96-97 -1.4179007870377518 1101 98-99 -1.514443123111974 1101 100 -1.6887357439693957 1102 1 -0.4287063257943977 1102 2 -0.36568001544184625 1102 3 -0.3773685743275017 1102 4 -0.35699347620663957 1102 5 -0.3362036643516433 1102 6 -0.3366267766603599 1102 7 -0.2756443864307627 1102 8 -0.2871417726217729 1102 9 -0.47632798005169974 1102 10-11 -0.4583749946944593 1102 12-13 -0.5198502536832663 1102 14-15 -0.6642999075080311 1102 16-17 -0.7125271890409337 1102 18-19 -0.7385765563897877 1102 20-21 -0.7593339924411353 1102 22-23 -0.7240569149343443 1102 24-25 -0.7342818228789483 1102 26-27 -0.758445488523499 1102 28-29 -0.8193853946316807 1102 30-31 -0.7981915598371785 1102 32-33 -0.9795680107821809 1102 34-35 -0.9058539881839565 1102 36-37 -0.9995403048951346 1102 38-39 -1.0764904951525338 1102 40-41 -1.0705868512583905 1102 42-43 -1.1709742441384492 1102 44-45 -1.3166973796106731 1102 46-47 -1.226656953641708 1102 48-49 -1.2218272220497894 1102 50-51 -1.2099844867465421 1102 52-53 -1.1634767988038774 1102 54-55 -0.9787682376618605 1102 56-57 -1.0636773334590472 1102 58-59 -0.9799094689914725 1102 60-61 -0.9625743459718663 1102 62-63 -1.0737737361966566 1102 64-65 -1.085985682826184 1102 66-67 -1.1506192381487423 1102 68-69 -1.145614216798755 1102 70-71 -1.1756378174299869 1102 72-73 -1.2521448099204697 1102 74-75 -1.2174095380922125 1102 76-77 -1.2042542495324184 1102 78-79 -1.1671736665354935 1102 80-81 -1.227479966507584 1102 82-83 -1.2580895000665464 1102 84-85 -1.215051193285987 1102 86-87 -1.1598617701430598 1102 88-89 -1.2328697982984984 1102 90-91 -1.3823792098755838 1102 92-93 -1.3359168111685698 1102 94-95 -1.4881321336168405 1102 96-97 -1.5001860309649864 1102 98-99 -1.5483225750382132 1102 100 -1.603601619182509 1103 1 -0.5312003330516433 1103 2 -0.44580694632544393 1103 3 -0.5274453288959471 1103 4 -0.5014237388024938 1103 5 -0.5076525058368944 1103 6 -0.45631459313234757 1103 7 -0.4826884094364843 1103 8 -0.43341439141183 1103 9 -0.6587868876189233 1103 10-11 -0.6693034753392411 1103 12-13 -0.655902618759491 1103 14-15 -0.8624239663746707 1103 16-17 -0.9099394845033331 1103 18-19 -0.7633125305363961 1103 20-21 -0.7842209408668666 1103 22-23 -0.8961116952908483 1103 24-25 -0.9377070475027907 1103 26-27 -1.120463434083696 1103 28-29 -0.9633730972585042 1103 30-31 -1.0718318436426273 1103 32-33 -1.099373324460423 1103 34-35 -1.136490178926067 1103 36-37 -1.0977403478567354 1103 38-39 -1.053247583046307 1103 40-41 -1.1467354423089375 1103 42-43 -1.2700198047010502 1103 44-45 -1.3862890486966322 1103 46-47 -1.2337037811709095 1103 48-49 -1.3167672376071877 1103 50-51 -1.4201675728195227 1103 52-53 -1.213355210383412 1103 54-55 -1.0160710195642437 1103 56-57 -0.9099617096744694 1103 58-59 -0.9713762440175273 1103 60-61 -1.0392110704980055 1103 62-63 -0.9108739529154377 1103 64-65 -0.9985738095832417 1103 66-67 -1.1282463061766208 1103 68-69 -0.9845243527780738 1103 70-71 -0.9642586483209215 1103 72-73 -0.9969743549897814 1103 74-75 -1.1682549093032506 1103 76-77 -1.0893919084888033 1103 78-79 -1.1510067443798206 1103 80-81 -1.183511394737362 1103 82-83 -1.105452192446073 1103 84-85 -1.1297205615408785 1103 86-87 -1.1999492743575502 1103 88-89 -1.271703665107676 1103 90-91 -1.2900938221825697 1103 92-93 -1.3844400369376437 1103 94-95 -1.4906399862825843 1103 96-97 -1.5137235967865372 1103 98-99 -1.6238988583850755 1103 100 -1.5491245210445292 1104 1 -0.5288345266511989 1104 2 -0.5585627277379324 1104 3 -0.5708909027012332 1104 4 -0.45422726681514547 1104 5 -0.41392054614401985 1104 6 -0.48901203909152 1104 7 -0.5576694972327658 1104 8 -0.46206421816012266 1104 9 -0.6779399652700775 1104 10-11 -0.6702665901230063 1104 12-13 -0.6103821310657835 1104 14-15 -0.8793435411474206 1104 16-17 -1.0226002572823703 1104 18-19 -0.9967714855496226 1104 20-21 -0.932005403534383 1104 22-23 -0.8772585005436468 1104 24-25 -0.939974035781681 1104 26-27 -0.9392006565843829 1104 28-29 -0.9823264624955357 1104 30-31 -1.0076698163610516 1104 32-33 -1.0362210591445091 1104 34-35 -1.1692153008163544 1104 36-37 -1.1685196655057979 1104 38-39 -1.0677845325286057 1104 40-41 -1.1427730032521737 1104 42-43 -1.1521454807804261 1104 44-45 -1.1638855329352893 1104 46-47 -1.244450404269216 1104 48-49 -1.227628015826184 1104 50-51 -1.2591407580612923 1104 52-53 -1.12709300925966 1104 54-55 -0.9212259274458674 1104 56-57 -0.9370814745711513 1104 58-59 -0.9966943877027319 1104 60-61 -0.9026247124120701 1104 62-63 -0.9720550557515821 1104 64-65 -0.876027396113443 1104 66-67 -1.0299067134733733 1104 68-69 -1.0237990513847066 1104 70-71 -1.0166020054079326 1104 72-73 -0.9494106102538282 1104 74-75 -1.0168089190447205 1104 76-77 -0.9247277944839283 1104 78-79 -0.9485634415627722 1104 80-81 -0.9595325985005587 1104 82-83 -0.9929151899356938 1104 84-85 -1.0545637801530034 1104 86-87 -1.0057850939532997 1104 88-89 -1.0090918594887874 1104 90-91 -1.1215552492488179 1104 92-93 -1.2223430798272226 1104 94-95 -1.1633318155324872 1104 96-97 -1.2364095289488475 1104 98-99 -1.2102287350650869 1104 100 -1.2148820914688194 1105 1 -0.5911367719383982 1105 2 -0.5334179155236463 1105 3 -0.6325651612640861 1105 4 -0.38632990986732096 1105 5 -0.25808905338698906 1105 6 -0.41350468900739656 1105 7 -0.33066528713858645 1105 8 -0.48422453529379084 1105 9 -0.6564327903734508 1105 10-11 -0.5966286700874264 1105 12-13 -0.5664306445608318 1105 14-15 -0.7153028096416278 1105 16-17 -0.7408302245490503 1105 18-19 -0.731174551428424 1105 20-21 -0.6987443979318684 1105 22-23 -0.6891722902738238 1105 24-25 -0.7656942221532432 1105 26-27 -0.7459585799419912 1105 28-29 -0.7629495728049775 1105 30-31 -0.7301871840836966 1105 32-33 -0.8298177121247008 1105 34-35 -0.7915026307617978 1105 36-37 -0.9018331597750944 1105 38-39 -0.8870957325400539 1105 40-41 -0.8808009597373427 1105 42-43 -0.8254930198447852 1105 44-45 -1.056530552870278 1105 46-47 -1.0357607215099023 1105 48-49 -1.0305202314753075 1105 50-51 -0.9886687794439339 1105 52-53 -0.7953069464574583 1105 54-55 -0.6485504948714791 1105 56-57 -0.657855433854742 1105 58-59 -0.5909084095281827 1105 60-61 -0.7053101084467599 1105 62-63 -0.7685693164305007 1105 64-65 -0.6996702815468652 1105 66-67 -0.6259305618005016 1105 68-69 -0.6501874995828061 1105 70-71 -0.6294663055716185 1105 72-73 -0.5929384103430451 1105 74-75 -0.53680398480968 1105 76-77 -0.6456645324619679 1105 78-79 -0.7843815388215667 1105 80-81 -0.6711928469446633 1105 82-83 -0.7963827534602181 1105 84-85 -0.7528061532174419 1105 86-87 -0.8381383760211989 1105 88-89 -0.8487130572658934 1105 90-91 -0.9651160255869868 1105 92-93 -0.9020285315419478 1105 94-95 -0.9037676802200103 1105 96-97 -0.8719970290018821 1105 98-99 -0.9851890954211839 1105 100 -1.017656001117203 1106 1 -0.6168484824195808 1106 2 -0.5390319385322506 1106 3 -0.6197612595522237 1106 4 -0.5295352025760209 1106 5 -0.4471114230072786 1106 6 -0.4870965193085084 1106 7 -0.49107140829925555 1106 8 -0.5257239234069786 1106 9 -0.5679234530389436 1106 10-11 -0.6000528360941786 1106 12-13 -0.6883854972596808 1106 14-15 -0.9434475775095166 1106 16-17 -0.9172965521520098 1106 18-19 -0.9503289846558545 1106 20-21 -0.7575629706079994 1106 22-23 -0.860174631317264 1106 24-25 -0.8616419470727905 1106 26-27 -0.9845406466522562 1106 28-29 -0.9328336238803487 1106 30-31 -0.9550347484240049 1106 32-33 -0.9916734127845288 1106 34-35 -1.0489013344572697 1106 36-37 -1.1023218456839388 1106 38-39 -1.085804328443352 1106 40-41 -1.1407205502908582 1106 42-43 -1.0305701241862053 1106 44-45 -1.0635963626147173 1106 46-47 -1.0931902931319968 1106 48-49 -0.9743582628902558 1106 50-51 -1.0723475514991847 1106 52-53 -1.0642785258925826 1106 54-55 -0.9227273133738976 1106 56-57 -0.8602731889704032 1106 58-59 -0.8863756579754423 1106 60-61 -0.966305346639686 1106 62-63 -0.9916927692549677 1106 64-65 -1.0880559802409593 1106 66-67 -1.0749193561978174 1106 68-69 -1.0340838087232598 1106 70-71 -0.9834803769094336 1106 72-73 -0.9796567550357267 1106 74-75 -0.9286303759632553 1106 76-77 -0.9484681533539714 1106 78-79 -0.9527467699398358 1106 80-81 -0.8938813999518977 1106 82-83 -0.8911443947139226 1106 84-85 -0.984632350727729 1106 86-87 -0.930836473564959 1106 88-89 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1107 46-47 -1.0619339499942484 1107 48-49 -1.0384739147619442 1107 50-51 -1.0404060664165442 1107 52-53 -0.9440239128508452 1107 54-55 -0.9643171609274148 1107 56-57 -0.9232402552699739 1107 58-59 -0.9789184797161923 1107 60-61 -0.9366804151520327 1107 62-63 -0.80961705424005 1107 64-65 -0.8351034053691322 1107 66-67 -0.8834971834568321 1107 68-69 -0.8474186062622948 1107 70-71 -0.7985474671398691 1107 72-73 -0.7632351157736323 1107 74-75 -0.7299844007457708 1107 76-77 -0.7224570742712828 1107 78-79 -0.6970731574150371 1107 80-81 -0.7917984805723357 1107 82-83 -0.888563519869102 1107 84-85 -0.830363031832956 1107 86-87 -0.8108936595476592 1107 88-89 -0.8744019298391201 1107 90-91 -0.9888539905475078 1107 92-93 -0.8933321814553068 1107 94-95 -0.9379042128850976 1107 96-97 -0.916135925132501 1107 98-99 -1.0083408848743751 1107 100 -0.9109901232534767 1108 1 -0.5555977871470894 1108 2 -0.5762454581984322 1108 3 -0.5414758098555197 1108 4 -0.39123086506924665 1108 5 -0.36912658999983705 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1.2558156164927823 2310 94-95 1.3170395962397414 2310 96-97 1.312174952968256 2310 98-99 1.4505452108102403 2310 100 1.4337548974858834 2311 1 0.7168723913376276 2311 2 0.7014819283063538 2311 3 0.7531911161380087 2311 4 0.6186101598385818 2311 5 0.5989258008950458 2311 6 0.5686682187608341 2311 7 0.6647507954262224 2311 8 0.5640876638021624 2311 9 0.7978260356147473 2311 10-11 0.8111140672525678 2311 12-13 0.9113903287552816 2311 14-15 1.1240333072506772 2311 16-17 1.1281092921750329 2311 18-19 1.0522006181775936 2311 20-21 1.0856326516051453 2311 22-23 1.1214670298427123 2311 24-25 1.134103856590123 2311 26-27 1.1922475801512817 2311 28-29 1.226107766348882 2311 30-31 1.230817813030761 2311 32-33 1.234087316502027 2311 34-35 1.2850446341613377 2311 36-37 1.3085245481270533 2311 38-39 1.303163513217008 2311 40-41 1.2346573394971685 2311 42-43 1.3612962501170713 2311 44-45 1.4299546658927937 2311 46-47 1.4340345553207143 2311 48-49 1.4513529767177644 2311 50-51 1.4367692136938288 2311 52-53 1.3985612162204433 2311 54-55 1.2781506828347702 2311 56-57 1.2372331372274772 2311 58-59 1.1983425768768399 2311 60-61 1.130703795434762 2311 62-63 1.2028516026531335 2311 64-65 1.187310992706223 2311 66-67 1.142258166635834 2311 68-69 1.165949134886283 2311 70-71 1.203313453872397 2311 72-73 1.157599333405237 2311 74-75 1.1544031304205689 2311 76-77 1.0923384630536361 2311 78-79 1.0844685920009667 2311 80-81 1.043887408688061 2311 82-83 1.1237572267265747 2311 84-85 1.172533040302369 2311 86-87 1.1317083950222653 2311 88-89 1.198159538887591 2311 90-91 1.2347321116294765 2311 92-93 1.2906216945722662 2311 94-95 1.323133686974284 2311 96-97 1.3049340668864424 2311 98-99 1.484306333819859 2311 100 1.539948468562713 2312 1 0.623857564683032 2312 2 0.6409759805966537 2312 3 0.653345543402903 2312 4 0.4362942348961454 2312 5 0.5246834073898867 2312 6 0.5571745451536572 2312 7 0.429354101714317 2312 8 0.5617646705825123 2312 9 0.759089741078995 2312 10-11 0.693278340990652 2312 12-13 0.8081904919022236 2312 14-15 1.0598546314529074 2312 16-17 1.055063416351068 2312 18-19 1.0428372507165093 2312 20-21 0.993592898418278 2312 22-23 1.0656436556265447 2312 24-25 1.0569424710227224 2312 26-27 1.0839523553668968 2312 28-29 1.1079485222630439 2312 30-31 1.1431008131834446 2312 32-33 1.1295387057245776 2312 34-35 1.0910897551103815 2312 36-37 1.1222685035221716 2312 38-39 1.1788771832152989 2312 40-41 1.158010450482287 2312 42-43 1.2457390289251933 2312 44-45 1.289441728205027 2312 46-47 1.3460034691372016 2312 48-49 1.3441079569881609 2312 50-51 1.3792077408584689 2312 52-53 1.2708019277176419 2312 54-55 1.251368033782562 2312 56-57 1.13742219406803 2312 58-59 1.1967141682393034 2312 60-61 1.1218071976393063 2312 62-63 1.1185569871784438 2312 64-65 1.1638848143841898 2312 66-67 1.1111654700596105 2312 68-69 1.0664965170789955 2312 70-71 1.0371931016828526 2312 72-73 1.1063920851823568 2312 74-75 1.1534325413583204 2312 76-77 1.1657371832897283 2312 78-79 1.1336545122490165 2312 80-81 1.1597849108386136 2312 82-83 1.2110029725027829 2312 84-85 1.2340782667773027 2312 86-87 1.2303626818825677 2312 88-89 1.1380148705431914 2312 90-91 1.1761024605268986 2312 92-93 1.2305459650644863 2312 94-95 1.358309648283118 2312 96-97 1.4668330275364951 2312 98-99 1.1357526079729219 2312 100 1.4062331065911131 2313 1 0.5871370739349899 2313 2 0.6545112891852831 2313 3 0.6414204669812698 2313 4 0.5512188997745824 2313 5 0.4002220819102291 2313 6 0.5322152093216701 2313 7 0.5532466898381401 2313 8 0.5551261145533459 2313 9 0.7047828342653588 2313 10-11 0.7403359084954886 2313 12-13 0.7805727132637159 2313 14-15 0.9593687556693737 2313 16-17 0.9300719551294208 2313 18-19 0.9079145998954203 2313 20-21 0.9842651311819353 2313 22-23 0.9939977470900487 2313 24-25 0.9850243928859115 2313 26-27 0.9572143525491938 2313 28-29 1.0403962739822603 2313 30-31 1.0393579219108133 2313 32-33 1.0804443069581282 2313 34-35 1.1035576847260131 2313 36-37 1.1169513644816504 2313 38-39 1.1573229566795575 2313 40-41 1.1748118864300707 2313 42-43 1.254032005659326 2313 44-45 1.2278806093632326 2313 46-47 1.220632984071699 2313 48-49 1.1933450285174487 2313 50-51 1.1740040037061803 2313 52-53 1.0960701732237155 2313 54-55 1.001621213600579 2313 56-57 1.0087386630002086 2313 58-59 1.0041667725189 2313 60-61 0.9383637466075712 2313 62-63 0.9498398272048121 2313 64-65 0.9338206702748195 2313 66-67 0.9549740326877938 2313 68-69 1.007717191112711 2313 70-71 0.9928251716041387 2313 72-73 0.9828862158824236 2313 74-75 0.9514540491039511 2313 76-77 0.8092081159980324 2313 78-79 0.9617426569089318 2313 80-81 1.0572320804809756 2313 82-83 1.0115156609143128 2313 84-85 1.0703206251034487 2313 86-87 1.1053584801700467 2313 88-89 1.118378756011385 2313 90-91 1.1900536436197093 2313 92-93 1.2652390589650615 2313 94-95 1.2691190931384604 2313 96-97 1.289358819024514 2313 98-99 1.3555734550146674 2313 100 1.5100915607972603 2314 1 0.5450535690632243 2314 2 0.5511232947777209 2314 3 0.5614444368763287 2314 4 0.44844681339272796 2314 5 0.4380857301734551 2314 6 0.4924035827523028 2314 7 0.5269243825642249 2314 8 0.5250653228366104 2314 9 0.6960085680631138 2314 10-11 0.642245665574741 2314 12-13 0.6918674855762887 2314 14-15 0.8517404585078907 2314 16-17 0.8465248622407628 2314 18-19 0.8343692119316586 2314 20-21 0.8322576323710749 2314 22-23 0.9563524292662748 2314 24-25 0.905474884337103 2314 26-27 0.936279922834963 2314 28-29 0.9599572743591054 2314 30-31 0.9352154524550755 2314 32-33 0.9581255380845306 2314 34-35 1.1101135720701905 2314 36-37 1.0559097491722866 2314 38-39 1.0767867848242005 2314 40-41 1.1454722707961693 2314 42-43 1.2355446032318795 2314 44-45 1.1847824240474054 2314 46-47 1.1799639928945567 2314 48-49 1.218586660415987 2314 50-51 1.1956518710113002 2314 52-53 1.1192557335952813 2314 54-55 0.9787962691870717 2314 56-57 1.0164002725270223 2314 58-59 1.0337868051943175 2314 60-61 0.9988886026213706 2314 62-63 0.9909968123741066 2314 64-65 1.0215526458084057 2314 66-67 0.9966511829824398 2314 68-69 1.049719659568808 2314 70-71 1.0170655230446783 2314 72-73 1.0425705242709569 2314 74-75 1.04225873077322 2314 76-77 0.9683449657229488 2314 78-79 0.9895622116247083 2314 80-81 1.0138052605071515 2314 82-83 1.0095158358287932 2314 84-85 1.0696665941174075 2314 86-87 1.0858871167665782 2314 88-89 1.0562655996504766 2314 90-91 1.1427744728215643 2314 92-93 1.2033408048805079 2314 94-95 1.1941349375053072 2314 96-97 1.1938791725536682 2314 98-99 1.2509570727187835 2314 100 1.3620557731865048 2315 1 0.38886538590382 2315 2 0.4129475828968445 2315 3 0.4694528487306151 2315 4 0.20512226732009964 2315 5 0.29991521932061715 2315 6 0.3158794236711202 2315 7 0.37944258922178165 2315 8 0.3788721606762522 2315 9 0.5039333351488438 2315 10-11 0.3765979138087019 2315 12-13 0.42619761851456417 2315 14-15 0.6718268681254784 2315 16-17 0.6845674715099861 2315 18-19 0.5300332197512034 2315 20-21 0.6513477828827021 2315 22-23 0.5043935822476513 2315 24-25 0.6818603114586423 2315 26-27 0.7505333795081199 2315 28-29 0.7823236136203278 2315 30-31 0.8180570293158738 2315 32-33 0.8656294939252618 2315 34-35 0.8348657933462249 2315 36-37 0.9180763436073107 2315 38-39 0.9647090667800597 2315 40-41 0.9890538438073193 2315 42-43 1.0498354941950865 2315 44-45 1.113782010237884 2315 46-47 1.2176482163670883 2315 48-49 1.1541472560639434 2315 50-51 1.0376345212558888 2315 52-53 1.14693350691892 2315 54-55 1.0035329704206148 2315 56-57 0.8960751663167841 2315 58-59 0.9594665183702986 2315 60-61 0.9564897783509068 2315 62-63 0.969609114282683 2315 64-65 1.0051443420306114 2315 66-67 1.006453438148192 2315 68-69 1.0335417962979747 2315 70-71 0.9167702313998802 2315 72-73 0.7159738042927373 2315 74-75 0.9234973857576421 2315 76-77 0.908813867683655 2315 78-79 0.9405589995746979 2315 80-81 0.6462235217623977 2315 82-83 0.7333013870407399 2315 84-85 0.9799584758108892 2315 86-87 1.0642914212597603 2315 88-89 1.0705155642048148 2315 90-91 1.1530128276323772 2315 92-93 1.2038143606686624 2315 94-95 1.247051835741555 2315 96-97 1.1221940805531645 2315 98-99 0.5595552199639933 2315 100 1.0356948477147014 2316 1 0.3035445671472843 2316 2 0.28415033097752485 2316 3 0.32675937209528527 2316 4 0.15388085199579393 2316 5 0.15795991074693916 2316 6 0.14215869450030993 2316 7 0.17522201795986803 2316 8 0.1572971246797934 2316 9 0.29425310659977555 2316 10-11 0.21687833768488218 2316 12-13 -0.03784791427217016 2316 14-15 0.01111405470399518 2316 16-17 0.20342198287446678 2316 18-19 0.31117464090315394 2316 20-21 0.34290182354417453 2316 22-23 0.332436330247468 2316 24-25 0.34101481281151536 2316 26-27 0.4039267551397714 2316 28-29 0.3277833021112997 2316 30-31 0.22650378632945234 2316 32-33 0.46050015322150983 2316 34-35 0.49677314165194275 2316 36-37 0.4772368483989453 2316 38-39 0.6268585523170032 2316 40-41 0.6662729164949042 2316 42-43 0.6150099085939118 2316 44-45 0.48294974200951657 2316 46-47 0.4541352602342954 2316 48-49 0.3181321853231296 2316 50-51 0.3812551270441631 2316 52-53 0.5057131801015586 2316 54-55 0.27332876033120357 2316 56-57 0.17912538213733598 2316 58-59 0.22895727997462956 2316 60-61 0.20374129334020807 2316 62-63 0.4113793952040652 2316 64-65 0.3151218264983626 2316 66-67 0.26859641483243735 2316 68-69 0.20153528445674596 2316 70-71 -0.05103774566715913 2316 72-73 -0.03735882937189672 2316 74-75 0.2431142789068943 2316 76-77 0.3087007912008133 2316 78-79 0.30318260954349086 2316 80-81 0.48611512848916405 2316 82-83 0.5420062281944098 2316 84-85 0.5711497874726028 2316 86-87 0.6305025934922668 2316 88-89 0.5993201709958385 2316 90-91 0.5244408035514674 2316 92-93 0.48642281173487945 2316 94-95 0.6342447055724989 2316 96-97 0.5259520024773465 2316 98-99 0.37001437491362665 2316 100 0.32113106785824996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18.0 1 372.0 2 115.0 3 131.0 4 96.0 5 62.0 6 97.0 7 513.0 8 1760.0 9 3042.0 10 4274.0 11 6020.0 12 8468.0 13 10867.0 14 13375.0 15 16038.0 16 19497.0 17 24997.0 18 30731.0 19 37071.0 20 45243.0 21 55052.0 22 61522.0 23 61409.0 24 59189.0 25 60239.0 26 65377.0 27 73999.0 28 87287.0 29 102891.0 30 124667.0 31 154412.0 32 194845.0 33 255414.0 34 358815.0 35 496014.0 36 742459.0 37 1077841.0 38 1046139.0 39 294728.0 40 449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.294688761540726 19.77056946180938 12.852802247634635 23.08193952901526 2 15.463199326431221 20.096579455257512 41.940769253482145 22.499451964829113 3 19.34168258083336 26.659172530062943 28.899262788590992 25.099882100512712 4 12.11582329185186 16.534974813329793 39.393124759367296 31.956077135451054 5 12.708762217213698 38.01837638393533 34.67909252416184 14.593768874689134 6 29.441320304328002 37.517938723145555 19.002583748312517 14.038157224213924 7 27.07627125463288 32.278038162360176 22.77287138956675 17.872819193440204 8 25.70796435651283 35.47133821851581 20.532374391430743 18.288323033540614 9 26.465648524688174 15.951743202523867 21.403955356794864 36.1786529159931 10-11 25.223189662986513 26.481275846601054 27.872073504600202 20.423460985812227 12-13 25.532136283885638 24.279052337890082 28.267418378177172 21.92139300004711 14-15 22.702629126342707 25.49934918013857 26.691988641665127 25.106033051853593 16-17 21.48955902662989 28.074581534810438 27.605917682146718 22.829941756412946 18-19 20.919002987191824 27.623114415595744 30.02382496400069 21.43405763321174 20-21 22.401288368194166 26.898261080103214 29.631699835021447 21.068750716681173 22-23 22.205342395229277 26.939465009688746 29.262725862155264 21.592466732926717 24-25 21.660231384157765 26.785406566848284 30.07115456118524 21.483207487808713 26-27 21.64376007693325 27.107602878562005 29.321417974210203 21.92721907029454 28-29 21.16326849072512 27.42047084996195 29.716449361795227 21.699811297517694 30-31 21.968770756518573 27.099442536661954 29.646046171434453 21.285740535385017 32-33 21.93065514154478 27.237029232758083 29.118453661173426 21.713861964523712 34-35 22.031899283971875 27.28707719970645 29.47682925554007 21.204194260781602 36-37 21.8027983209538 27.149138467684885 29.08886315809231 21.959200053269004 38-39 21.82267204313905 27.373927147708827 29.40636169423409 21.39703911491803 40-41 21.970889969117056 26.566444802494587 29.727403715480804 21.73526151290755 42-43 21.7420455483996 26.75902331622983 29.27578924184801 22.223141893522563 44-45 21.839077890999263 27.434968663204767 29.10781091187348 21.618142533922494 46-47 21.74368314894303 27.197105921271774 28.615037144169513 22.44417378561568 48-49 22.051843572142943 27.183244817126695 28.755949501244878 22.008962109485484 50-51 22.03564974217251 27.31518951668082 28.52748954741937 22.1216711937273 52-53 21.761051738785923 27.668357729065775 28.331951925579908 22.238638606568397 54-55 21.692595334538186 27.53645445140035 28.467421127464853 22.30352908659661 56-57 22.252734507096832 27.333244478222497 28.104800044266653 22.30922097041402 58-59 21.690788421521912 27.588231860276156 28.599537091874595 22.121442626327333 60-61 21.875393820743614 27.998789110142535 28.12968283587759 21.996134233236265 62-63 21.946571981296028 27.53751895298373 28.073913865772298 22.441995199947947 64-65 21.55306752757023 27.468758505020226 28.26906275291612 22.709111214493426 66-67 21.787245940350648 27.314477673749792 28.117920379037965 22.7803560068616 68-69 21.551840963310887 27.402234357211103 28.287075640900948 22.758849038577058 70-71 21.58482477482555 27.048913833926797 28.3784212628935 22.98784012835415 72-73 21.85915248187102 27.177844068400265 28.378602474124797 22.584400975603913 74-75 22.013001685363935 27.334393508524425 28.04158315522724 22.611021650884407 76-77 21.78367556697576 27.204397274047594 27.98211596469668 23.02981119427997 78-79 21.662784229439886 27.20474887137621 28.03187602512788 23.100590874056024 80-81 21.40720476225121 27.509531813617805 28.193530718904945 22.889732705226038 82-83 22.051355660228456 27.024785145179276 28.737832162719624 22.18602703187264 84-85 22.144156628395688 27.177440680818325 28.045309601519914 22.633093089266072 86-87 21.35088906665585 27.566388317690148 28.375321318840967 22.70740129681304 88-89 21.98430424139401 26.77782037910414 28.28168890724023 22.95618647226162 90-91 21.912091267095466 26.875042564939573 28.285025739164194 22.927840428800767 92-93 21.97587414896767 26.966341943375898 28.3137116739958 22.74407223366063 94-95 21.270904492682845 27.018250161058173 28.625623228985752 23.08522211727323 96-97 21.518079527738305 26.940074307497614 28.332412585815646 23.20943357894843 98-99 21.23606987548258 27.152772421130965 28.14965544111126 23.46150226227519 100 21.68239485590786 26.77766674591839 27.782066110236165 23.75787228793759 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1303.0 1 929.0 2 1275.0 3 1887.5 4 1335.0 5 1005.0 6 1370.5 7 2428.5 8 3915.0 9 4648.5 10 4299.0 11 4029.0 12 4306.0 13 4823.0 14 5468.0 15 6141.0 16 6881.0 17 7538.0 18 8242.0 19 8963.0 20 9837.0 21 11021.0 22 12583.5 23 14702.0 24 17308.5 25 20547.0 26 24985.0 27 30253.0 28 36401.0 29 45663.5 30 58619.0 31 72002.0 32 88156.5 33 106605.5 34 123443.5 35 139308.5 36 155909.0 37 177677.0 38 188417.0 39 186559.0 40 182109.0 41 175873.5 42 175523.5 43 181877.0 44 191745.5 45 208567.5 46 223384.0 47 232310.5 48 238885.5 49 237438.0 50 229986.5 51 227101.5 52 220035.5 53 205407.0 54 190335.5 55 166832.0 56 145374.5 57 120934.0 58 97403.5 59 85906.5 60 69733.0 61 51032.0 62 38636.5 63 30577.0 64 22159.5 65 13121.5 66 8366.0 67 5831.0 68 4372.5 69 3422.0 70 2672.0 71 2017.5 72 1527.0 73 1263.5 74 1001.5 75 720.5 76 532.0 77 360.0 78 228.5 79 168.0 80 132.5 81 100.0 82 70.5 83 55.0 84 48.0 85 31.5 86 29.5 87 29.5 88 27.5 89 23.0 90 18.5 91 16.0 92 8.0 93 7.5 94 7.5 95 4.5 96 5.5 97 6.5 98 5.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05659869878394113 2 0.13206958762656296 3 0.016781237182860977 4 0.016245095419830276 5 0.01915813233229709 6 0.05155896621145252 7 0.056580827391840095 8 0.04832424424116729 9 0.0394779051511607 10-11 0.039281319838049444 12-13 0.04029105349175727 14-15 0.018818575882377647 16-17 0.018112655894387222 18-19 0.017567578435306007 20-21 0.020936335846348922 22-23 0.02528801982294812 24-25 0.02457316413890718 26-27 0.01921174650860016 28-29 0.018121591590437733 30-31 0.01620935263562823 32-33 0.02257156822359256 34-35 0.02272347505645126 36-37 0.023009417330067634 38-39 0.02073975053323766 40-41 0.024063829464028016 42-43 0.03125706478468994 44-45 0.04056806006932313 46-47 0.046429876678458805 48-49 0.0394779051511607 50-51 0.028263606607768516 52-53 0.039442162366958654 54-55 0.03660061102289593 56-57 0.03859327124216005 58-59 0.04318621901212306 60-61 0.023268552515532476 62-63 0.02553821931236245 64-65 0.02524334134269556 66-67 0.0320702131252865 68-69 0.03858433554610953 70-71 0.04039828184436341 72-73 0.0444372164591947 74-75 0.0266462456226259 76-77 0.02073081483718715 78-79 0.019855116624237005 80-81 0.024555292746806158 82-83 0.021016757110803523 84-85 0.013198023066605783 86-87 0.01464560582678868 88-89 0.01840753386405411 90-91 0.02040912977936873 92-93 0.019292167773054765 94-95 0.02292899606561303 96-97 0.02409957224823006 98-99 0.026780281063383576 100 0.02761130079608116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5595535.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.13948300304905 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4914559806308 61.15873898572745 2 12.394801276070638 18.378883170668196 3 3.0210779483215133 6.7194347160140735 4 0.9924034687261583 2.9430512040715966 5 0.403718566744974 1.4965742908602162 6 0.20838241475605768 0.9269618698164632 7 0.11633784978067144 0.6037659625497753 8 0.07302638767804057 0.43313109024241264 9 0.04502459541738089 0.3004290203999463 >10 0.2245961884493948 2.936287407405879 >50 0.01781365194970756 0.9249750054123793 >100 0.010324378837541595 1.436555233242205 >500 6.824302793014443E-4 0.33410909982628456 >1k 2.729710444572774E-4 0.41180144258488227 >5k 0.0 0.0 >10k+ 8.189131333438758E-5 0.9953015011783559 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21497 0.38418131599570016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16887 0.3017941984099823 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10796 0.19293954912264868 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.7871392101023405E-5 3 0.0 0.0 0.0 0.0 1.7871392101023405E-5 4 0.0 0.0 0.0 0.0 1.7871392101023405E-5 5 0.0 0.0 0.0 0.0 1.7871392101023405E-5 6 0.0 0.0 0.0 0.0 1.7871392101023405E-5 7 0.0 0.0 0.0 0.0 1.7871392101023405E-5 8 0.0 0.0 0.0 0.0 1.7871392101023405E-5 9 0.0 0.0 0.0 0.0 1.7871392101023405E-5 10-11 0.0 0.0 0.0 2.6807088151535106E-5 3.574278420204681E-5 12-13 0.0 0.0 0.0 3.574278420204681E-5 3.574278420204681E-5 14-15 0.0 0.0 0.0 3.574278420204681E-5 3.574278420204681E-5 16-17 0.0 0.0 0.0 4.4678480252558514E-5 4.4678480252558514E-5 18-19 8.935696050511702E-6 0.0 0.0 7.148556840409362E-5 5.361417630307022E-5 20-21 1.7871392101023405E-5 0.0 0.0 1.6084252890921066E-4 5.361417630307022E-5 22-23 1.7871392101023405E-5 0.0 0.0 3.038136657173979E-4 5.361417630307022E-5 24-25 1.7871392101023405E-5 0.0 0.0 7.059199879904244E-4 5.361417630307022E-5 26-27 1.7871392101023405E-5 0.0 0.0 0.0014922612404354545 5.361417630307022E-5 28-29 1.7871392101023405E-5 0.0 0.0 0.003610021204406728 5.361417630307022E-5 30-31 1.7871392101023405E-5 0.0 0.0 0.013153344586353225 5.361417630307022E-5 32-33 1.7871392101023405E-5 0.0 0.0 0.037485244931896596 5.361417630307022E-5 34-35 1.7871392101023405E-5 0.0 0.0 0.07465774050202528 5.361417630307022E-5 36-37 1.7871392101023405E-5 0.0 0.0 0.13618000780979833 5.361417630307022E-5 38-39 1.7871392101023405E-5 0.0 0.0 0.22932570344033235 5.361417630307022E-5 40-41 1.7871392101023405E-5 0.0 0.0 0.3328368064894599 5.361417630307022E-5 42-43 1.7871392101023405E-5 0.0 0.0 0.43087032785962376 5.361417630307022E-5 44-45 1.7871392101023405E-5 0.0 0.0 0.5485266377567114 5.361417630307022E-5 46-47 1.7871392101023405E-5 0.0 0.0 0.6808821676568907 5.361417630307022E-5 48-49 1.7871392101023405E-5 0.0 0.0 0.8282854097061318 5.361417630307022E-5 50-51 1.7871392101023405E-5 0.0 0.0 0.9832035721338532 5.361417630307022E-5 52-53 1.7871392101023405E-5 0.0 0.0 1.1317773903657113 6.254987235358192E-5 54-55 1.7871392101023405E-5 0.0 0.0 1.2975702948869055 7.148556840409362E-5 56-57 1.7871392101023405E-5 0.0 0.0 1.4895090460518967 7.148556840409362E-5 58-59 1.7871392101023405E-5 0.0 0.0 1.7043142434101477 8.042126445460533E-5 60-61 1.7871392101023405E-5 0.0 0.0 1.9236140958818058 9.829265655562873E-5 62-63 1.7871392101023405E-5 0.0 0.0 2.150455675820096 1.0722835260614044E-4 64-65 1.7871392101023405E-5 0.0 0.0 2.381613196950783 1.0722835260614044E-4 66-67 1.7871392101023405E-5 0.0 0.0 2.6362447916061647 1.0722835260614044E-4 68-69 1.7871392101023405E-5 0.0 0.0 2.9165128982304642 1.0722835260614044E-4 70-71 1.7871392101023405E-5 0.0 0.0 3.216430600469839 1.2509974470716384E-4 72-73 1.7871392101023405E-5 0.0 0.0 3.516080231827698 1.9658531311125745E-4 74-75 1.7871392101023405E-5 8.935696050511702E-6 0.0 3.8099484678408766 1.9658531311125745E-4 76-77 1.7871392101023405E-5 1.7871392101023405E-5 0.0 4.132312638559137 1.9658531311125745E-4 78-79 1.7871392101023405E-5 1.7871392101023405E-5 0.0 4.486952543411846 1.9658531311125745E-4 80-81 1.7871392101023405E-5 1.7871392101023405E-5 0.0 4.861018651478366 2.1445670521228085E-4 82-83 1.7871392101023405E-5 1.7871392101023405E-5 0.0 5.246585000361895 2.3232809731330427E-4 84-85 1.7871392101023405E-5 1.7871392101023405E-5 0.0 5.628505585256816 2.3232809731330427E-4 86-87 1.7871392101023405E-5 1.7871392101023405E-5 0.0 6.028663925790831 2.3232809731330427E-4 88 1.7871392101023405E-5 1.7871392101023405E-5 0.0 6.346774705189048 2.3232809731330427E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10265 0.0 50.933216 1 TACCTGG 4950 0.0 47.344986 2 GTATCAA 18775 0.0 41.40722 1 GTACCTG 5940 0.0 40.483723 1 ACCTGGG 5890 0.0 39.026684 3 ATCAACG 21675 0.0 34.35296 3 CAACGCA 21800 0.0 33.96191 5 TATCAAC 23045 0.0 33.22467 2 GTACTAG 3595 0.0 32.07105 1 AACGCAG 24015 0.0 31.319105 6 TCAACGC 24005 0.0 31.096882 4 GCATCGT 3230 0.0 30.123997 8 GTGGTAT 3825 0.0 30.019564 1 GTACATG 23895 0.0 29.876144 1 TACATGG 24055 0.0 29.149477 2 CCTGGGG 5200 0.0 28.294962 4 ACGCAGA 26320 0.0 28.094078 7 ACATGGG 24070 0.0 28.063948 3 CGCAGAG 26520 0.0 27.720943 8 CTGTGCG 4755 0.0 27.480677 9 >>END_MODULE