FastQCFastQC Report
Mon 6 Jun 2016
ERR1211230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5595535
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199980.3573920992362661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166700.2979161063240602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105740.1889721000762215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106550.049.5171
TACCTGG52350.043.545362
GTATCAA187400.039.7466431
ACCTGGG58050.038.303113
GTACCTG63750.036.43861
GTACTAG36350.033.3756831
ATCAACG220900.032.53773
CAACGCA223400.032.307595
TATCAAC239500.030.8506182
AACGCAG245400.029.9282256
TCAACGC244750.029.539884
CCTGGGG50150.029.3392164
CTGTGCG45900.028.561969
GTACATG237550.028.287341
TACATGG236200.027.998262
GGGCACC42300.027.89384894
CTGGGGG38900.027.541135
ACATGGG233050.027.1702483
ACGCAGA269200.026.8284367
GTGGTAT40550.026.6717721
Produced by FastQC (version 0.11.5)