##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211230_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5595535 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14960410398648 33.0 31.0 34.0 30.0 34.0 2 32.27761634946435 34.0 31.0 34.0 30.0 34.0 3 32.359524871169604 34.0 31.0 34.0 30.0 34.0 4 35.976412621849384 37.0 35.0 37.0 35.0 37.0 5 35.86942910731503 37.0 35.0 37.0 35.0 37.0 6 35.89778350059467 37.0 35.0 37.0 35.0 37.0 7 35.840075345789096 37.0 35.0 37.0 35.0 37.0 8 35.82916754162024 37.0 35.0 37.0 35.0 37.0 9 37.43739445826002 39.0 37.0 39.0 34.0 39.0 10-11 37.412491030795096 39.0 37.0 39.0 34.0 39.0 12-13 37.31047165284463 39.0 37.0 39.0 34.0 39.0 14-15 38.527108131751476 40.0 38.0 41.0 34.0 41.0 16-17 38.4864868149337 40.0 38.0 41.0 34.0 41.0 18-19 38.52568967936042 40.0 38.0 41.0 34.0 41.0 20-21 38.49440652234326 40.0 38.0 41.0 34.0 41.0 22-23 38.3633955466278 40.0 38.0 41.0 34.0 41.0 24-25 38.30115842363599 40.0 38.0 41.0 34.0 41.0 26-27 38.166098684040044 40.0 38.0 41.0 33.5 41.0 28-29 38.143116699296854 40.0 37.5 41.0 33.5 41.0 30-31 38.145056102767654 40.0 37.5 41.0 34.0 41.0 32-33 38.00187819395286 40.0 37.0 41.0 33.0 41.0 34-35 37.85199717274577 40.0 37.0 41.0 33.0 41.0 36-37 37.7041917350173 40.0 37.0 41.0 33.0 41.0 38-39 37.614254222339774 40.0 37.0 41.0 33.0 41.0 40-41 37.51428782055693 40.0 36.5 41.0 32.5 41.0 42-43 37.36507733398147 40.0 36.0 41.0 32.0 41.0 44-45 37.21866792004697 40.0 36.0 41.0 31.5 41.0 46-47 36.97126548221037 39.0 36.0 41.0 31.0 41.0 48-49 36.72900705651917 39.0 35.5 41.0 30.5 41.0 50-51 36.53041541157369 39.0 35.0 41.0 30.0 41.0 52-53 36.334712945232226 39.0 35.0 40.0 30.0 41.0 54-55 36.106160268857224 38.5 35.0 40.0 29.0 41.0 56-57 35.86274994258815 38.0 35.0 40.0 28.5 41.0 58-59 35.60175336227903 38.0 34.5 40.0 28.0 41.0 60-61 35.354832022317794 37.5 34.0 40.0 28.0 41.0 62-63 35.0624084202851 37.0 34.0 40.0 27.5 41.0 64-65 34.70212231716896 36.5 34.0 39.5 26.5 41.0 66-67 34.41040472448121 36.0 33.5 39.0 26.0 41.0 68-69 34.086761140802444 36.0 33.0 39.0 26.0 40.0 70-71 33.768309464599895 35.0 33.0 38.5 26.0 40.0 72-73 33.43009113516402 35.0 32.5 38.0 26.0 40.0 74-75 33.08920183682168 35.0 32.0 37.0 25.0 39.0 76-77 32.07806286619599 34.0 30.5 36.0 24.5 39.0 78-79 32.567980899056124 35.0 32.0 36.0 25.0 39.0 80-81 32.48473068258889 35.0 32.0 36.0 25.0 37.5 82-83 32.22182400789201 35.0 32.0 35.5 25.0 37.0 84-85 31.959001686165845 34.0 31.5 35.0 25.0 37.0 86-87 31.713960863438437 34.0 31.0 35.0 24.0 36.0 88-89 31.489385644089438 34.0 31.0 35.0 24.0 36.0 90-91 31.269928344653373 34.0 31.0 35.0 23.0 35.5 92-93 31.090428618532457 34.0 31.0 35.0 23.0 35.0 94-95 30.902633438983045 34.0 31.0 35.0 20.5 35.0 96-97 30.73557345276189 34.0 30.0 35.0 20.0 35.0 98-99 30.563524846149654 34.0 30.0 35.0 20.0 35.0 100 28.800099901081843 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33768507446682605 1101 2 -0.3329520862478148 1101 3 -0.40057707975429224 1101 4 -0.19464251107394404 1101 5 -0.24116429596043076 1101 6 -0.2910033573529063 1101 7 -0.2994750066397316 1101 8 -0.3308766910391441 1101 9 -0.41282308888258257 1101 10-11 -0.40273144148966367 1101 12-13 -0.4213697675309547 1101 14-15 -0.6342448103809204 1101 16-17 -0.5277831714567398 1101 18-19 -0.6854496983834863 1101 20-21 -0.6391205537414137 1101 22-23 -0.6863111433261224 1101 24-25 -0.6956566109257452 1101 26-27 -0.8011424737402209 1101 28-29 -0.7992846033172469 1101 30-31 -0.5776006309536541 1101 32-33 -0.6042567354973585 1101 34-35 -0.583533657883379 1101 36-37 -0.53829971070639 1101 38-39 -0.63640205766729 1101 40-41 -0.6532744955474996 1101 42-43 -0.6154637689038154 1101 44-45 -0.6322736361274721 1101 46-47 -0.5557003220518837 1101 48-49 -0.5766789846436993 1101 50-51 -0.6373512182741834 1101 52-53 -0.7395295878538732 1101 54-55 -0.6367120550336551 1101 56-57 -0.7163519044828419 1101 58-59 -0.7302857831408858 1101 60-61 -0.7391076168831887 1101 62-63 -0.823335774872497 1101 64-65 -0.7692157785151323 1101 66-67 -0.8024417318933317 1101 68-69 -0.980809157574484 1101 70-71 -0.9479238215966888 1101 72-73 -0.920821763974871 1101 74-75 -0.9730745124603075 1101 76-77 -0.9143981761128614 1101 78-79 -0.8063551284570174 1101 80-81 -0.7740445323154965 1101 82-83 -0.7532646324939734 1101 84-85 -0.6273633795566411 1101 86-87 -0.7476217001668743 1101 88-89 -0.7679034472728219 1101 90-91 -0.763695061559595 1101 92-93 -0.8410912175753005 1101 94-95 -0.8658111729357287 1101 96-97 -0.9621915171890585 1101 98-99 -1.075238795519443 1101 100 -0.9717935543537557 1102 1 -0.42393677546630926 1102 2 -0.3611318994898056 1102 3 -0.38909341717002377 1102 4 -0.2674294129868855 1102 5 -0.3077805191941323 1102 6 -0.3539960997551077 1102 7 -0.4252632537108809 1102 8 -0.35979946957292697 1102 9 -0.4459207067012372 1102 10-11 -0.5367953834288883 1102 12-13 -0.5099743222992288 1102 14-15 -0.6548901343919908 1102 16-17 -0.7098499835881924 1102 18-19 -0.7639906709641622 1102 20-21 -0.7350823418633539 1102 22-23 -0.7336043643543917 1102 24-25 -0.7124412917634899 1102 26-27 -0.8031432792710191 1102 28-29 -0.84368147191406 1102 30-31 -0.6921217311679442 1102 32-33 -0.6611535649907339 1102 34-35 -0.6675957619610884 1102 36-37 -0.7492515274520528 1102 38-39 -0.8573914528153423 1102 40-41 -0.7858745221378385 1102 42-43 -0.7892329882428086 1102 44-45 -0.8422964334308958 1102 46-47 -0.8366317268037307 1102 48-49 -0.8194681154525156 1102 50-51 -0.8349993029878675 1102 52-53 -0.9381206128331456 1102 54-55 -0.7359203277566593 1102 56-57 -0.9104370013811618 1102 58-59 -1.019958643791881 1102 60-61 -0.8802497891572898 1102 62-63 -0.8418134389896181 1102 64-65 -0.8552819337088522 1102 66-67 -0.9629296254694211 1102 68-69 -0.9570627355389121 1102 70-71 -1.0816415417099279 1102 72-73 -1.0372914275289062 1102 74-75 -1.1649895250516096 1102 76-77 -0.9681143711920832 1102 78-79 -0.7242245386207813 1102 80-81 -0.766718659917828 1102 82-83 -0.6951150853117625 1102 84-85 -0.7317305295417782 1102 86-87 -0.6860776227733503 1102 88-89 -0.8854845869033738 1102 90-91 -0.8306473397258003 1102 92-93 -0.9128139585697745 1102 94-95 -0.9730650559069645 1102 96-97 -1.1144324178194047 1102 98-99 -1.0028622059647567 1102 100 -1.0095473747594923 1103 1 -0.3744608207109792 1103 2 -0.3715702742468743 1103 3 -0.40228199702713496 1103 4 -0.3067190148616632 1103 5 -0.3186709307440836 1103 6 -0.32073468241674163 1103 7 -0.37593908223183803 1103 8 -0.3853237151407001 1103 9 -0.599686719451654 1103 10-11 -0.5783541067604787 1103 12-13 -0.5812368787055888 1103 14-15 -0.7054872436371227 1103 16-17 -0.795493466091358 1103 18-19 -0.7421716323794669 1103 20-21 -0.7259222596630721 1103 22-23 -0.7359803119597217 1103 24-25 -0.815965113879038 1103 26-27 -0.8320498649085266 1103 28-29 -0.937944048660917 1103 30-31 -0.8684992530046998 1103 32-33 -0.7733184013205943 1103 34-35 -0.8606709080890838 1103 36-37 -0.8888342405542247 1103 38-39 -0.8008279895308661 1103 40-41 -0.8658459329487513 1103 42-43 -0.77911848201461 1103 44-45 -0.8250752338838154 1103 46-47 -0.9024951517494415 1103 48-49 -0.9876900213696942 1103 50-51 -1.0522616535237148 1103 52-53 -0.9768287383697825 1103 54-55 -1.0325887156610918 1103 56-57 -0.9856842540268147 1103 58-59 -0.9509626662798496 1103 60-61 -1.0648634413926885 1103 62-63 -1.0827470078412844 1103 64-65 -1.0342896979242369 1103 66-67 -1.0881701287673522 1103 68-69 -1.2130266625867847 1103 70-71 -1.1577523795597813 1103 72-73 -1.0739623441273025 1103 74-75 -0.9932542600488006 1103 76-77 -0.9817081045305933 1103 78-79 -0.8258759560762599 1103 80-81 -0.8500929053931472 1103 82-83 -0.9109945900390812 1103 84-85 -0.8324126913547722 1103 86-87 -0.8080963141518787 1103 88-89 -0.9277711765350176 1103 90-91 -0.849302351902363 1103 92-93 -1.0906940963181064 1103 94-95 -1.041347074165337 1103 96-97 -1.1425053511475625 1103 98-99 -1.1457178133783152 1103 100 -1.2660369958652886 1104 1 -0.39463937446624797 1104 2 -0.3611536480560176 1104 3 -0.4451291764402363 1104 4 -0.26840173585407 1104 5 -0.3476510782063187 1104 6 -0.4110086935631543 1104 7 -0.393100826163689 1104 8 -0.4591302615631605 1104 9 -0.5639661399187972 1104 10-11 -0.5881358817579425 1104 12-13 -0.6549769876554237 1104 14-15 -0.8308986778766325 1104 16-17 -0.7809219264570189 1104 18-19 -0.8876414500718397 1104 20-21 -0.8798029699619434 1104 22-23 -0.8799395342847305 1104 24-25 -0.9585981581560361 1104 26-27 -0.9035551948014344 1104 28-29 -0.8991482979468159 1104 30-31 -0.8760129730666009 1104 32-33 -0.8604485181202577 1104 34-35 -0.9456005693196943 1104 36-37 -0.8581376329872015 1104 38-39 -0.833307113726498 1104 40-41 -0.9329498374226404 1104 42-43 -0.8145484928419364 1104 44-45 -0.8289698259573512 1104 46-47 -0.9757382748805625 1104 48-49 -1.0167280159088676 1104 50-51 -0.8854010267699692 1104 52-53 -0.8879061812249844 1104 54-55 -0.9414061606480786 1104 56-57 -0.9731953234468804 1104 58-59 -0.9382130714760422 1104 60-61 -0.8942017835933598 1104 62-63 -1.0284719545713088 1104 64-65 -1.0058962497256942 1104 66-67 -1.058166886131076 1104 68-69 -0.9736344488909623 1104 70-71 -1.1770699699549354 1104 72-73 -1.1335587951472306 1104 74-75 -1.0718469520944254 1104 76-77 -0.9493848645883851 1104 78-79 -0.7936893434250862 1104 80-81 -0.7519091588484486 1104 82-83 -0.7641118332070107 1104 84-85 -0.8366637753255652 1104 86-87 -0.7961640640693446 1104 88-89 -0.9544475551106686 1104 90-91 -1.0321316099596025 1104 92-93 -1.0193136795403 1104 94-95 -0.9978374247700543 1104 96-97 -1.0118721372721566 1104 98-99 -1.0186710420832945 1104 100 -1.1273597049934239 1105 1 -0.43136995227973784 1105 2 -0.43908674047463236 1105 3 -0.43856231107880816 1105 4 -0.23583683585155057 1105 5 -0.29730772610810163 1105 6 -0.35776637512747556 1105 7 -0.39088248539952986 1105 8 -0.47714262238136484 1105 9 -0.5523643423734441 1105 10-11 -0.5721270261163838 1105 12-13 -0.5894379037043151 1105 14-15 -0.8219111388287956 1105 16-17 -0.8368918158339724 1105 18-19 -0.7549068667498204 1105 20-21 -0.8648673160738625 1105 22-23 -0.8295723783133013 1105 24-25 -0.9009762086420992 1105 26-27 -0.9144990255076877 1105 28-29 -0.8811464311074033 1105 30-31 -0.797519363261415 1105 32-33 -0.7840086681988012 1105 34-35 -0.8246447732248683 1105 36-37 -0.8595052481030478 1105 38-39 -0.8618001246803004 1105 40-41 -0.8884211257652268 1105 42-43 -0.8767811850138187 1105 44-45 -0.8285047493368509 1105 46-47 -0.7385515825672826 1105 48-49 -0.8581853971576692 1105 50-51 -0.8533381857461677 1105 52-53 -0.9136843867215703 1105 54-55 -0.8675604448139893 1105 56-57 -0.8434687574871234 1105 58-59 -0.9600686314507101 1105 60-61 -0.9759142477481646 1105 62-63 -1.0254769091935785 1105 64-65 -0.830394816818881 1105 66-67 -0.8999753745096584 1105 68-69 -0.86336561568136 1105 70-71 -0.9550258450080307 1105 72-73 -0.8839217009633771 1105 74-75 -0.9485325450930233 1105 76-77 -0.9424675115986076 1105 78-79 -0.783848394222737 1105 80-81 -0.839418898631191 1105 82-83 -0.7149359409437928 1105 84-85 -0.7678968260949119 1105 86-87 -0.8304142895584796 1105 88-89 -0.8041130299692192 1105 90-91 -0.8015174148389512 1105 92-93 -0.8410472427875071 1105 94-95 -0.8980465008715228 1105 96-97 -0.8316209681133877 1105 98-99 -0.8701165322836992 1105 100 -1.0400220176538433 1106 1 -0.4558399715695032 1106 2 -0.36414530551186175 1106 3 -0.48567141901650146 1106 4 -0.3506587713918563 1106 5 -0.3687493369264274 1106 6 -0.3841839190903471 1106 7 -0.45274936320909376 1106 8 -0.42453230163204125 1106 9 -0.5869037753614208 1106 10-11 -0.5472998610945083 1106 12-13 -0.6246473906820356 1106 14-15 -0.7700166119484919 1106 16-17 -0.6896817240362978 1106 18-19 -0.7095571861147079 1106 20-21 -0.7767878705406233 1106 22-23 -0.7574441314078797 1106 24-25 -0.7707535481710792 1106 26-27 -0.7494841199828031 1106 28-29 -0.7653939134572099 1106 30-31 -0.8356328688677763 1106 32-33 -0.8131015499421466 1106 34-35 -0.8641741562376737 1106 36-37 -0.8856796065196306 1106 38-39 -0.7734091620049739 1106 40-41 -0.8887311961471411 1106 42-43 -0.8261409064612906 1106 44-45 -0.8502027761377491 1106 46-47 -0.9040599631481498 1106 48-49 -0.8832291495965237 1106 50-51 -0.9372169445728389 1106 52-53 -0.8318635700462238 1106 54-55 -0.7912001090869865 1106 56-57 -0.8716762034572128 1106 58-59 -0.8719184245065037 1106 60-61 -0.9475424275647271 1106 62-63 -0.981192897262332 1106 64-65 -0.9828894412701814 1106 66-67 -1.0795016555202679 1106 68-69 -1.0681827112524402 1106 70-71 -0.9733708752737016 1106 72-73 -1.0757749907272185 1106 74-75 -1.0567900103847876 1106 76-77 -1.0027984680039026 1106 78-79 -0.800492034526556 1106 80-81 -0.8750607257735474 1106 82-83 -0.8241775370199989 1106 84-85 -0.8746901886411749 1106 86-87 -0.8492496231366751 1106 88-89 -0.774915499240123 1106 90-91 -0.9145613934175536 1106 92-93 -0.9028194876432849 1106 94-95 -0.8845782580177683 1106 96-97 -0.8758043374716671 1106 98-99 -0.8548272635308223 1106 100 -0.8332175929567285 1107 1 -0.4171918816343414 1107 2 -0.4056060290244119 1107 3 -0.4117714506265102 1107 4 -0.21572317023056087 1107 5 -0.2765456526954182 1107 6 -0.3980664425362832 1107 7 -0.420962845435092 1107 8 -0.34298416585092184 1107 9 -0.5194153370855901 1107 10-11 -0.5171506250842555 1107 12-13 -0.5313972387051678 1107 14-15 -0.6801066284800612 1107 16-17 -0.6914783664641888 1107 18-19 -0.7167183740455414 1107 20-21 -0.6434572101395446 1107 22-23 -0.6854055797502525 1107 24-25 -0.7831529449449093 1107 26-27 -0.771759798388878 1107 28-29 -0.8209254470801497 1107 30-31 -0.783146170582242 1107 32-33 -0.738039686697185 1107 34-35 -0.849764688563944 1107 36-37 -0.872750533515422 1107 38-39 -0.7578674269367056 1107 40-41 -0.9667296114379695 1107 42-43 -0.778115476095202 1107 44-45 -0.8406925090136141 1107 46-47 -0.9510866804351323 1107 48-49 -0.91017922904242 1107 50-51 -0.9847846906684907 1107 52-53 -0.9872474721566533 1107 54-55 -0.9760286858930627 1107 56-57 -1.0019713320972485 1107 58-59 -1.0805672395299268 1107 60-61 -1.0931196587303162 1107 62-63 -1.185760968023267 1107 64-65 -1.0359270811034875 1107 66-67 -1.0479734412066293 1107 68-69 -0.9643757874866168 1107 70-71 -0.9157876630899153 1107 72-73 -0.9302829500475553 1107 74-75 -1.1151517879260844 1107 76-77 -1.1088696496666444 1107 78-79 -0.8163185254855847 1107 80-81 -0.7306859633931637 1107 82-83 -0.7679084734567105 1107 84-85 -0.8838103147208791 1107 86-87 -0.926657556680194 1107 88-89 -0.8326325057125317 1107 90-91 -0.972748082820388 1107 92-93 -0.9290565697936408 1107 94-95 -0.9183578455134338 1107 96-97 -1.0700701741707164 1107 98-99 -1.085371528654985 1107 100 -1.209268555585222 1108 1 -0.3717615618701693 1108 2 -0.41744929670505826 1108 3 -0.3840123312549011 1108 4 -0.2697368548641492 1108 5 -0.2926788952731272 1108 6 -0.37017947128331485 1108 7 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0.9472563857916754 2311 34-35 0.9749424737944494 2311 36-37 0.9939481722805183 2311 38-39 0.990618956212387 2311 40-41 0.9947674864797946 2311 42-43 0.9633409475955474 2311 44-45 0.9469838277251483 2311 46-47 0.6669221826928222 2311 48-49 0.9775043551683424 2311 50-51 1.094734925675084 2311 52-53 1.0082725295403137 2311 54-55 0.9787003337802815 2311 56-57 1.07611686433399 2311 58-59 1.1042202725455112 2311 60-61 1.1741055935833487 2311 62-63 1.1840937024863294 2311 64-65 1.1657411190222007 2311 66-67 1.2310076084217556 2311 68-69 1.2732155682237334 2311 70-71 1.2533437185522445 2311 72-73 1.2808120899416977 2311 74-75 1.2767465989810916 2311 76-77 1.217448484303759 2311 78-79 0.9305353204031093 2311 80-81 0.8911916356430964 2311 82-83 0.9653117286063448 2311 84-85 1.0419478194616474 2311 86-87 1.004919325298257 2311 88-89 1.0428285705017046 2311 90-91 1.1382959597134352 2311 92-93 1.1596816541691517 2311 94-95 1.1383332946288078 2311 96-97 1.1754281293021513 2311 98-99 1.2345956544382553 2311 100 1.3684261119546761 2312 1 0.25117491555436544 2312 2 0.37275862906781754 2312 3 0.4789944113754032 2312 4 0.2085327329414426 2312 5 0.3759260122382102 2312 6 0.4140671885366345 2312 7 0.2835662711357614 2312 8 0.404135706178387 2312 9 0.6216141079577611 2312 10-11 0.6405251130146112 2312 12-13 0.7024673124322405 2312 14-15 0.8722336474509973 2312 16-17 0.875912770953029 2312 18-19 0.843669832154859 2312 20-21 0.8776687118703066 2312 22-23 0.9117092232042836 2312 24-25 0.9362360360365969 2312 26-27 1.00395558252864 2312 28-29 0.9362321661128234 2312 30-31 0.9491671817499494 2312 32-33 0.8262502916269909 2312 34-35 0.9390361594312182 2312 36-37 1.008316100666697 2312 38-39 0.8079495106379468 2312 40-41 0.943541837958243 2312 42-43 0.9956310160254418 2312 44-45 0.7269189960344917 2312 46-47 0.7595131618485169 2312 48-49 0.8950951830643348 2312 50-51 0.7149805572057204 2312 52-53 0.9691382262229595 2312 54-55 0.7812127041551591 2312 56-57 0.8583818948332933 2312 58-59 0.9580005017982884 2312 60-61 1.0150801403113903 2312 62-63 1.0605665182694466 2312 64-65 1.0578945483289246 2312 66-67 0.9694559044722482 2312 68-69 1.050474080319347 2312 70-71 0.9425186484478161 2312 72-73 1.0076878082028742 2312 74-75 1.0944615310192134 2312 76-77 1.0874943640426835 2312 78-79 0.9338534957341125 2312 80-81 0.890314222561372 2312 82-83 0.9153320329978172 2312 84-85 0.9414735401963625 2312 86-87 0.9126561348288256 2312 88-89 0.9127918802349626 2312 90-91 0.9807991548523383 2312 92-93 1.0945636377854164 2312 94-95 1.1766525394549276 2312 96-97 0.9000967726662772 2312 98-99 0.6734475113914122 2312 100 0.8362033451975037 2313 1 0.05506904083108566 2313 2 0.20986320529778624 2313 3 0.2670884387784227 2313 4 0.17101415296291833 2313 5 0.3441386026954021 2313 6 0.25780587429532886 2313 7 0.23684935624954306 2313 8 0.3495148487875852 2313 9 0.5689509778947013 2313 10-11 0.37567580019129565 2313 12-13 0.4239029648551522 2313 14-15 0.5076401374201822 2313 16-17 0.7367117803556695 2313 18-19 0.6780051832087253 2313 20-21 0.6861163876534278 2313 22-23 0.6738663758460319 2313 24-25 0.5861855889916612 2313 26-27 0.7335789471236396 2313 28-29 0.5589325967851835 2313 30-31 0.7738161771944974 2313 32-33 0.8720447880620767 2313 34-35 0.9083963616995305 2313 36-37 0.9122145664650816 2313 38-39 1.0110842542768026 2313 40-41 1.002621855636427 2313 42-43 1.048409236725341 2313 44-45 0.8850696006629448 2313 46-47 0.8896850379820975 2313 48-49 0.9888439886433176 2313 50-51 0.9594596682263372 2313 52-53 0.922182029712431 2313 54-55 0.8777749925648166 2313 56-57 0.5964524928652466 2313 58-59 0.6833077774410654 2313 60-61 0.5942664611435333 2313 62-63 0.9370256874958969 2313 64-65 1.0366580737826396 2313 66-67 1.018059667626197 2313 68-69 1.0376468711648883 2313 70-71 1.0346790627013078 2313 72-73 1.0209045814807283 2313 74-75 1.0926501811033802 2313 76-77 0.9976553801173367 2313 78-79 0.8604416814377558 2313 80-81 0.7698866447758945 2313 82-83 0.8206647831714733 2313 84-85 0.8591017049844041 2313 86-87 0.8643151874970734 2313 88-89 0.8824101753586859 2313 90-91 0.7443998304531512 2313 92-93 0.9292001874061349 2313 94-95 1.0086727958677884 2313 96-97 1.1107518847905489 2313 98-99 1.1673040374751729 2313 100 1.1200866864435355 2314 1 -0.28042741502893875 2314 2 0.08673044310461364 2314 3 0.30205610438873975 2314 4 0.19841011178618828 2314 5 0.31128728621600743 2314 6 0.3769275516627033 2314 7 0.4601569971940549 2314 8 0.4789276261120179 2314 9 0.5059440647972266 2314 10-11 0.5797125973570019 2314 12-13 0.436466065947819 2314 14-15 0.7680474152563477 2314 16-17 0.8388507285630737 2314 18-19 0.8641196218589613 2314 20-21 0.8303444910600533 2314 22-23 0.88960673199891 2314 24-25 0.8094322376663214 2314 26-27 0.7142856472015353 2314 28-29 0.7327961428511856 2314 30-31 0.804853850227957 2314 32-33 0.8306594447970994 2314 34-35 0.8390924266551636 2314 36-37 0.8536990941935159 2314 38-39 0.6344433869010615 2314 40-41 0.6376378036298291 2314 42-43 0.8656036566316843 2314 44-45 0.9001418268111152 2314 46-47 0.8245695812455907 2314 48-49 0.8398031909117734 2314 50-51 0.8226876520604165 2314 52-53 0.8121032324344313 2314 54-55 0.78552855056261 2314 56-57 0.743693828802769 2314 58-59 0.9262175433196376 2314 60-61 0.9653070946805826 2314 62-63 0.8370045624178815 2314 64-65 0.9246536538419008 2314 66-67 0.655021461094627 2314 68-69 0.6663171887619797 2314 70-71 0.7726478740550959 2314 72-73 0.9316035792466337 2314 74-75 0.9611956925703566 2314 76-77 0.8467106342004982 2314 78-79 0.767932086042741 2314 80-81 0.8437738775505892 2314 82-83 0.7211211777631874 2314 84-85 0.796779062174835 2314 86-87 0.8925543404428602 2314 88-89 0.842831273047139 2314 90-91 0.9565002367273259 2314 92-93 0.8922060754046761 2314 94-95 0.8999839679766275 2314 96-97 0.9301813777042547 2314 98-99 1.0822022112044714 2314 100 1.1838606594094188 2315 1 0.39007338540490366 2315 2 0.4242911050110436 2315 3 0.38937668858918784 2315 4 0.30369749437188887 2315 5 0.3580857431452884 2315 6 0.41628670284094227 2315 7 0.34111320139113843 2315 8 0.22299355906368845 2315 9 0.4593565772993671 2315 10-11 0.5433727303669613 2315 12-13 0.3795734625439522 2315 14-15 0.5617492281686367 2315 16-17 0.6899757808574449 2315 18-19 0.6890337874138908 2315 20-21 0.43270942036190974 2315 22-23 0.8060165742320535 2315 24-25 0.8022248147241342 2315 26-27 0.7316785842579066 2315 28-29 0.6934662092128079 2315 30-31 0.7923249233337799 2315 32-33 0.7281617363088628 2315 34-35 0.786532126635386 2315 36-37 0.8104802775908624 2315 38-39 0.8382670210018404 2315 40-41 0.8285031637865998 2315 42-43 0.936502235186353 2315 44-45 0.939760701595354 2315 46-47 0.9478833555083881 2315 48-49 0.7833194800945265 2315 50-51 0.8096900426102422 2315 52-53 0.9166226985274903 2315 54-55 0.7178522492610213 2315 56-57 0.7478019635078184 2315 58-59 0.7287607323942211 2315 60-61 0.8656140951943883 2315 62-63 0.9193958613689546 2315 64-65 1.0045818508089468 2315 66-67 1.0820113962749573 2315 68-69 1.0733668413595296 2315 70-71 0.9429615030387595 2315 72-73 1.0103196307380315 2315 74-75 0.3915213660860104 2315 76-77 0.4241812143013135 2315 78-79 0.6783583859020297 2315 80-81 0.7402747963406 2315 82-83 0.6738772025204227 2315 84-85 0.7222643049789284 2315 86-87 0.8050651817454231 2315 88-89 0.7799569192202327 2315 90-91 0.8227092974251597 2315 92-93 0.8057545436538369 2315 94-95 0.925224491485011 2315 96-97 0.9456808127589724 2315 98-99 0.9345296406571144 2315 100 0.9712492765530918 2316 1 0.229352496347488 2316 2 0.07322576272230208 2316 3 0.15258179268995775 2316 4 -0.028788567410089172 2316 5 0.261939858528919 2316 6 0.36263338622230634 2316 7 0.4495793201539229 2316 8 0.4394276562049484 2316 9 0.6770352453059516 2316 10-11 0.573743571040886 2316 12-13 0.353191931090457 2316 14-15 0.474732756596147 2316 16-17 0.7566913562123361 2316 18-19 0.7141565524624127 2316 20-21 0.711568886541059 2316 22-23 0.7485153104774369 2316 24-25 0.3883722160218426 2316 26-27 0.6919769999917307 2316 28-29 0.8420492416490433 2316 30-31 0.49487617824146213 2316 32-33 0.6251339156001947 2316 34-35 0.7802442606926121 2316 36-37 0.8201004695549869 2316 38-39 0.7731388409921962 2316 40-41 0.7671705656327887 2316 42-43 0.6765275392851393 2316 44-45 0.5599504353107676 2316 46-47 0.7605503453762807 2316 48-49 0.8196756305384127 2316 50-51 0.8346484931886309 2316 52-53 0.815535161973969 2316 54-55 0.6821591250090862 2316 56-57 0.8155370027494726 2316 58-59 0.7943423236535665 2316 60-61 0.8169231524848684 2316 62-63 0.8119791248013399 2316 64-65 0.934637427445395 2316 66-67 0.8987473090477138 2316 68-69 0.8992448464739624 2316 70-71 0.953807104087133 2316 72-73 0.9715426793193913 2316 74-75 0.9332571536040035 2316 76-77 0.7979429611103726 2316 78-79 0.5386389297590739 2316 80-81 0.5912405171448327 2316 82-83 0.4419667698711436 2316 84-85 0.563187890081366 2316 86-87 0.5803718954973327 2316 88-89 0.6313546700666279 2316 90-91 0.5231408901743784 2316 92-93 0.31025576029508883 2316 94-95 0.41178279871920864 2316 96-97 0.6021037604684949 2316 98-99 0.7344211172947297 2316 100 0.5811383553311309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 5.0 12 22.0 13 135.0 14 539.0 15 1562.0 16 3817.0 17 8170.0 18 13661.0 19 20041.0 20 27582.0 21 38221.0 22 46713.0 23 47252.0 24 44889.0 25 47328.0 26 54456.0 27 66650.0 28 82578.0 29 100946.0 30 125118.0 31 157211.0 32 200106.0 33 262192.0 34 374627.0 35 520264.0 36 809159.0 37 1237554.0 38 1124638.0 39 180076.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68493845278042 20.501370973970804 13.660125039691733 23.15356553355704 2 15.273821001923855 20.544326860612973 41.55659825199914 22.625253885464037 3 18.9155639273099 26.61018115336603 29.581049890671757 24.89320502865231 4 12.098789180716137 16.922615403978284 39.292344349796046 31.68625106550953 5 12.605783003769972 38.165126301595826 34.7297622121924 14.499328482441804 6 29.61825812902609 37.698611482190714 18.703323274718144 13.979807114065053 7 27.070290104755685 32.385922534838734 22.630165404461017 17.913621955944567 8 25.86660614221875 35.89817595636521 20.152836145247953 18.082381756168086 9 26.401430591128687 15.948517307757616 21.272714923796535 36.37733717731716 10-11 25.251379854869505 26.647908789823855 27.75114037740219 20.349570977904452 12-13 25.524460621232876 24.32307405128143 28.2559244340286 21.896540893457093 14-15 22.63609034999193 25.623797142325593 26.590801334422537 25.149311173259935 16-17 21.45323593278515 28.192359976584836 27.52882909263385 22.825574997996164 18-19 20.896652420188598 27.660045018036705 30.034491786754973 21.408810775019727 20-21 22.370970782954625 26.90752537514286 29.645753265773518 21.075750576129 22-23 22.143217762019184 26.929087209712744 29.30192555314192 21.625769475126148 24-25 21.586619123768568 26.787996728804604 30.09883106234501 21.52655308508182 26-27 21.581500167098262 27.11412364556582 29.39294184086884 21.91143434646708 28-29 21.138891730940472 27.45720577729267 29.7069400516752 21.696962440091664 30-31 21.950494615096673 27.01919242221953 29.710120515711846 21.32019244697195 32-33 21.946988386813096 27.216593152379904 29.20275241779411 21.633666043012894 34-35 21.991392903010663 27.28018398655227 29.550538428447304 21.177884681989763 36-37 21.81131122935305 27.08434188970511 29.237500992169192 21.866845888772648 38-39 21.791110541088756 27.259440262873653 29.569532324959813 21.379916871077775 40-41 21.84536664314763 26.553766369497602 29.9368435312292 21.664023456125562 42-43 21.70914028289533 26.724730845545086 29.398846311767468 22.16728255979212 44-45 21.74662330088168 27.347817748597965 29.261127168430747 21.64443178208961 46-47 21.69126406879825 27.14096780784051 28.727955598303424 22.43981252505782 48-49 22.07475978761692 27.12109191882347 28.87644782883535 21.92770046472426 50-51 22.009529184299858 27.27769444321565 28.563388110727278 22.14938826175721 52-53 21.668159086603254 27.65381755657791 28.376988850640046 22.301034506178794 54-55 21.68724496078891 27.50087444568544 28.431727910036386 22.380152683489268 56-57 22.249764141291116 27.255357017437724 28.16600664353979 22.328872197731357 58-59 21.59961559139016 27.571487704929755 28.644446700391484 22.1844500032886 60-61 21.882906200427072 27.976243241639576 28.11219682547399 22.02865373245936 62-63 21.951508073275377 27.508443217657863 28.0613907595746 22.478657949492156 64-65 21.493003334639894 27.454692295556093 28.229324539883606 22.822979829920403 66-67 21.663247134661074 27.32514094113711 28.168270838021837 22.843341086179986 68-69 21.45741820595231 27.366123880958792 28.305820315199753 22.870637597889143 70-71 21.449484773323775 27.00906397350587 28.439266245761953 23.102185007408405 72-73 21.64113270692037 27.202074947492488 28.406900471642366 22.749891873944776 74-75 21.832541893398922 27.347405124640616 28.024392868852665 22.795660113107797 76-77 21.520080767307295 27.245415610632932 28.002130912578505 23.232372709481265 78-79 21.39091710175184 27.237782611726473 28.111352014141982 23.259948272379702 80-81 21.099563701650062 27.555467508345572 28.245756443188192 23.099212346816174 82-83 21.758967578054882 27.020650512785487 28.759226098317797 22.46115581084183 84-85 21.89874934177059 27.20184778070523 28.02526706903966 22.87413580848452 86-87 20.98727450081049 27.577648970059805 28.387885875527395 23.04719065360231 88-89 21.53736717811791 26.8762278451357 28.306818882950424 23.279586093795967 90-91 21.45006076555118 26.88910952943665 28.336227229010408 23.324602476001765 92-93 21.487983551969965 27.047918296198624 28.32605537801417 23.13804277381724 94-95 20.721237285136457 27.07807028989263 28.63045883431668 23.57023359065423 96-97 20.885409764533584 26.999308263847706 28.405844397522472 23.70943757409624 98-99 20.558466734652107 27.227606140854988 28.284505665105865 23.92942145938704 100 20.80731894810233 26.862809946904555 28.111625578776124 24.218245526216993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1273.0 1 876.5 2 1084.0 3 1590.5 4 1108.5 5 796.5 6 1179.5 7 2162.5 8 3370.0 9 4043.0 10 3785.5 11 3468.0 12 3697.0 13 4170.0 14 4859.5 15 5669.0 16 6505.5 17 7222.5 18 8045.0 19 8980.5 20 10001.5 21 11322.0 22 12931.0 23 14998.5 24 17692.0 25 20937.0 26 25604.5 27 30997.5 28 37083.5 29 46306.0 30 59169.0 31 72761.0 32 89263.5 33 107509.5 34 123994.5 35 140252.5 36 156324.5 37 177948.5 38 189315.5 39 187117.5 40 182602.5 41 176952.0 42 177314.5 43 183228.5 44 192714.0 45 210753.5 46 226551.0 47 235335.0 48 242351.5 49 240343.5 50 231960.0 51 227757.5 52 219892.5 53 203583.0 54 188342.5 55 166320.0 56 144003.0 57 118909.5 58 95337.0 59 85095.5 60 68690.0 61 48857.5 62 36373.0 63 28700.0 64 20397.0 65 11436.5 66 7132.0 67 5058.5 68 3762.5 69 2866.5 70 2205.0 71 1607.5 72 1208.5 73 973.5 74 731.5 75 531.0 76 337.0 77 192.5 78 119.5 79 76.5 80 52.5 81 33.5 82 22.0 83 13.5 84 8.5 85 5.5 86 2.5 87 3.0 88 2.5 89 2.0 90 2.0 91 1.5 92 1.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0434453541975879 2 0.0 3 0.0 4 0.035153028262713035 5 0.0 6 0.0 7 6.969842919399128E-4 8 0.0 9 0.011527047905160097 10-11 3.3955644991944466E-4 12-13 8.935696050511702E-6 14-15 1.0722835260614042E-4 16-17 2.770065775658628E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.8252758672763193E-4 26-27 4.4678480252558514E-4 28-29 1.3403544075767554E-4 30-31 0.0022071169244763907 32-33 0.014609863042586635 34-35 0.02556502640051398 36-37 0.030935379726871513 38-39 0.021669062922490878 40-41 0.0011973832707685682 42-43 4.4678480252558514E-5 44-45 0.011580662081463168 46-47 0.017406735906396794 48-49 0.0031096222255780725 50-51 0.011035584622381953 52-53 0.010633478300108926 54-55 0.010043722360775154 56-57 4.289134104245617E-4 58-59 0.007988512269157462 60-61 0.004342748280548688 62-63 0.00120631896681908 64-65 0.03534067787977378 66-67 0.060682311879024974 68-69 0.05903814380573082 70-71 0.051818101396917365 72-73 0.04514313644718512 74-75 0.03454540093127824 76-77 0.030157974170476998 78-79 0.015101326325364777 80-81 9.293123892532171E-4 82-83 0.03865582111451363 84-85 0.05146960925094741 86-87 0.05754588256529536 88-89 0.07023457095702199 90-91 0.04875315765159185 92-93 0.03860220693821056 94-95 0.012599331431221501 96-97 0.0034581143715480293 98-99 0.02144567052122809 100 0.03279400450537795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5595535.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.2101573611948 #Duplication Level Percentage of deduplicated Percentage of total 1 81.58646391814767 59.72957861991034 2 12.99742055787885 19.03086408663878 3 3.1881855171769717 7.002226902276251 4 1.0453103953886151 3.061093541507731 5 0.4379683710000237 1.603186668006894 6 0.22250184811932286 0.9773637188383376 7 0.12319027691489279 0.6313145690815926 8 0.0804657629834965 0.4712728936168307 9 0.05176104799924695 0.3410491022284704 >10 0.23598456943492271 3.0411345688526277 >50 0.01870615462824031 0.9624131827264021 >100 0.011012305811789476 1.5049069523277876 >500 7.23274950113359E-4 0.348377036981251 >1k 2.2254513883752724E-4 0.3331014839467564 >5k 0.0 0.0 >10k+ 8.345442706363958E-5 0.9621166730599 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19998 0.3573920992362661 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16670 0.2979161063240602 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10574 0.1889721000762215 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7871392101023405E-5 0.0 12-13 0.0 0.0 0.0 1.7871392101023405E-5 0.0 14-15 0.0 0.0 0.0 1.7871392101023405E-5 0.0 16-17 0.0 0.0 0.0 2.6807088151535106E-5 0.0 18-19 0.0 0.0 0.0 5.361417630307021E-5 0.0 20-21 0.0 0.0 0.0 1.4297113680818724E-4 0.0 22-23 0.0 0.0 0.0 2.859422736163745E-4 1.7871392101023405E-5 24-25 0.0 0.0 0.0 6.880485958894011E-4 3.574278420204681E-5 26-27 0.0 0.0 0.0 0.0014565184562334074 3.574278420204681E-5 28-29 0.0 0.0 0.0 0.0036546996846592863 3.574278420204681E-5 30-31 0.0 0.0 0.0 0.013358865595514996 4.4678480252558514E-5 32-33 0.0 0.0 0.0 0.037905222646270643 5.361417630307022E-5 34-35 0.0 0.0 0.0 0.07523856074530853 7.148556840409362E-5 36-37 0.0 0.0 0.0 0.13702889893459697 7.148556840409362E-5 38-39 0.0 1.7871392101023405E-5 0.0 0.23071073632816164 8.042126445460533E-5 40-41 0.0 3.574278420204681E-5 0.0 0.3349992449336837 8.935696050511703E-5 42-43 0.0 3.574278420204681E-5 0.0 0.4334795511063732 8.935696050511703E-5 44-45 0.0 3.574278420204681E-5 0.0 0.5517077455506936 1.0722835260614044E-4 46-47 0.0 3.574278420204681E-5 0.0 0.6853232085939951 1.0722835260614044E-4 48-49 0.0 4.4678480252558514E-5 0.0 0.8332268496220647 1.2509974470716384E-4 50-51 0.0 5.361417630307022E-5 0.0 0.98873476798912 1.2509974470716384E-4 52-53 0.0 5.361417630307022E-5 0.0 1.137013708251311 1.3403544075767554E-4 54-55 0.0 5.361417630307022E-5 0.0 1.3023062137936767 1.4297113680818724E-4 56-57 0.0 5.361417630307022E-5 0.0 1.494173479390264 1.4297113680818724E-4 58-59 0.0 5.361417630307022E-5 0.0 1.7095684326878484 1.4297113680818724E-4 60-61 0.0 7.148556840409362E-5 0.0 1.9284483074451326 1.4297113680818724E-4 62-63 0.0 7.148556840409362E-5 0.0 2.1549682023256045 1.4297113680818724E-4 64-65 0.0 7.148556840409362E-5 0.0 2.385678938653766 1.4297113680818724E-4 66-67 0.0 7.148556840409362E-5 0.0 2.640096076603935 1.4297113680818724E-4 68-69 0.0 7.148556840409362E-5 0.0 2.920140790826972 1.4297113680818724E-4 70-71 0.0 8.042126445460533E-5 0.0 3.2201210429387004 1.4297113680818724E-4 72-73 0.0 8.935696050511703E-5 0.0 3.5203336231477422 1.4297113680818724E-4 74-75 0.0 8.935696050511703E-5 0.0 3.815381371039588 1.4297113680818724E-4 76-77 0.0 8.935696050511703E-5 0.0 4.13898760350887 1.5190683285869894E-4 78-79 0.0 8.935696050511703E-5 0.0 4.494619370623184 1.6084252890921063E-4 80-81 0.0 8.935696050511703E-5 0.0 4.869409270069797 1.7871392101023406E-4 82-83 0.0 8.935696050511703E-5 0.0 5.2555743105887105 1.9658531311125745E-4 84-85 0.0 8.935696050511703E-5 0.0 5.636788975495641 1.9658531311125745E-4 86-87 0.0 8.935696050511703E-5 0.0 6.037108158558565 1.9658531311125745E-4 88 0.0 8.935696050511703E-5 0.0 6.35588911516057 1.9658531311125745E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10655 0.0 49.517 1 TACCTGG 5235 0.0 43.54536 2 GTATCAA 18740 0.0 39.746643 1 ACCTGGG 5805 0.0 38.30311 3 GTACCTG 6375 0.0 36.4386 1 GTACTAG 3635 0.0 33.375683 1 ATCAACG 22090 0.0 32.5377 3 CAACGCA 22340 0.0 32.30759 5 TATCAAC 23950 0.0 30.850618 2 AACGCAG 24540 0.0 29.928225 6 TCAACGC 24475 0.0 29.53988 4 CCTGGGG 5015 0.0 29.339216 4 CTGTGCG 4590 0.0 28.56196 9 GTACATG 23755 0.0 28.28734 1 TACATGG 23620 0.0 27.99826 2 GGGCACC 4230 0.0 27.893848 94 CTGGGGG 3890 0.0 27.54113 5 ACATGGG 23305 0.0 27.170248 3 ACGCAGA 26920 0.0 26.828436 7 GTGGTAT 4055 0.0 26.671772 1 >>END_MODULE