FastQCFastQC Report
Mon 6 Jun 2016
ERR1211229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979922
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286750.35933935193852773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227270.2848022825285761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139500.1748137387809054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151500.046.877021
GTATCAA265600.040.9501271
GTACTAG57000.038.3112641
TACCTGG51000.034.14342
GCATCGT58500.034.0698
CAACGCA316950.033.0883065
ATCAACG322250.032.6024553
TATCAAC335800.032.2065732
GTGGTAT53950.030.5323331
AACGCAG353350.030.1852746
GGGCACC67000.029.6022394
TCAACGC362600.029.1948554
TAGGCAT82800.029.1241155
GTACATG374000.028.3638841
CTGTGCG78750.028.3522179
ACCTGGG61000.028.2044053
CTAGGCA87000.027.8261874
TACATGG383400.027.2505972
CGCAGAG385400.027.1129728
GTACCTG67300.026.9932771
Produced by FastQC (version 0.11.5)