FastQCFastQC Report
Mon 6 Jun 2016
ERR1211229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979922
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266930.3345020164357496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231410.28999030316336427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141620.1774704063523428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157300.049.0772551
GTATCAA274850.040.0443731
TACCTGG47650.035.702762
GGGCACC69800.034.21588594
GTACTAG58950.033.8954121
CAACGCA329700.032.2063985
ATCAACG331250.032.052363
GTGGTAT53200.031.8146171
TATCAAC349750.031.3885462
CTGTGCG86750.030.3838869
AACGCAG366200.029.471036
TCAACGC370350.028.9729824
ACCTGGG58200.028.7492583
CTAGTAC99150.027.35163
ACTGTGC106200.026.94288
GTACATG384050.026.4914251
GTACTGT103250.026.3931146
CGCAGAG405600.026.0288568
ACGCAGA410100.025.8694297
TACATGG388550.025.7867372
Produced by FastQC (version 0.11.5)