##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211229_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7979922 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16017174102704 33.0 31.0 34.0 30.0 34.0 2 32.30010005110326 34.0 31.0 34.0 30.0 34.0 3 32.372605772337124 34.0 31.0 34.0 30.0 34.0 4 35.98823271706164 37.0 35.0 37.0 35.0 37.0 5 35.87692461154382 37.0 35.0 37.0 35.0 37.0 6 35.905144436248875 37.0 35.0 37.0 35.0 37.0 7 35.84566315309849 37.0 35.0 37.0 35.0 37.0 8 35.83407369144711 37.0 35.0 37.0 35.0 37.0 9 37.43318593840892 39.0 37.0 39.0 34.0 39.0 10-11 37.41065695880235 39.0 37.0 39.0 34.0 39.0 12-13 37.30760607685138 39.0 37.0 39.0 34.0 39.0 14-15 38.52122821501263 40.0 38.0 41.0 34.0 41.0 16-17 38.47699043925492 40.0 38.0 41.0 34.0 41.0 18-19 38.50170101913277 40.0 38.0 41.0 34.0 41.0 20-21 38.467914222720474 40.0 38.0 41.0 34.0 41.0 22-23 38.34579109921125 40.0 38.0 41.0 34.0 41.0 24-25 38.28001927838392 40.0 38.0 41.0 34.0 41.0 26-27 38.13944271635738 40.0 37.5 41.0 33.5 41.0 28-29 38.1201252343068 40.0 37.5 41.0 33.5 41.0 30-31 38.12659547298833 40.0 37.5 41.0 33.5 41.0 32-33 37.994979336890765 40.0 37.0 41.0 33.0 41.0 34-35 37.85838859076567 40.0 37.0 41.0 33.0 41.0 36-37 37.72900756423434 40.0 37.0 41.0 33.0 41.0 38-39 37.639856442205826 40.0 37.0 41.0 33.0 41.0 40-41 37.539575449484346 40.0 36.0 41.0 32.5 41.0 42-43 37.39649040429217 40.0 36.0 41.0 32.0 41.0 44-45 37.24565302768624 39.5 36.0 41.0 31.5 41.0 46-47 36.99647552946006 39.0 36.0 41.0 31.0 41.0 48-49 36.751439989012425 39.0 35.0 41.0 30.5 41.0 50-51 36.54681988119684 39.0 35.0 41.0 30.0 41.0 52-53 36.34223436018547 39.0 35.0 40.0 30.0 41.0 54-55 36.10593487004009 38.0 35.0 40.0 29.0 41.0 56-57 35.84808216170534 38.0 35.0 40.0 29.0 41.0 58-59 35.57752030658946 38.0 34.0 40.0 28.0 41.0 60-61 35.31482951838376 37.0 34.0 40.0 28.0 41.0 62-63 35.01081207310047 37.0 34.0 40.0 27.5 41.0 64-65 34.6418364114336 36.0 34.0 39.0 27.0 41.0 66-67 34.338128179699 36.0 33.0 39.0 26.0 40.5 68-69 34.01719434099732 35.0 33.0 39.0 26.0 40.0 70-71 33.71008400833993 35.0 33.0 38.0 26.0 40.0 72-73 33.373360165675805 35.0 32.5 37.0 26.0 39.5 74-75 33.03863358564156 35.0 32.0 37.0 25.5 39.0 76-77 32.04593935880577 34.0 30.5 36.0 24.5 38.0 78-79 32.5422331822291 35.0 32.0 36.0 25.0 38.0 80-81 32.47650171267338 35.0 32.0 35.5 25.0 37.0 82-83 32.22996264875772 35.0 32.0 35.0 25.0 37.0 84-85 31.98578638989203 34.0 32.0 35.0 25.0 36.5 86-87 31.757952959941214 34.0 31.0 35.0 24.5 36.0 88-89 31.55549903871241 34.0 31.0 35.0 24.0 36.0 90-91 31.348407916769112 34.0 31.0 35.0 24.0 35.0 92-93 31.17084214607611 34.0 31.0 35.0 23.0 35.0 94-95 30.99052954402311 34.0 31.0 35.0 23.0 35.0 96-97 30.827590232084976 34.0 30.0 35.0 21.0 35.0 98-99 30.662797330600476 34.0 30.0 35.0 20.0 35.0 100 28.871969926523093 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3829395406998586 1101 2 -0.33512451221101003 1101 3 -0.41172030296491613 1101 4 -0.23147529612735696 1101 5 -0.26027669467730874 1101 6 -0.3983671410209624 1101 7 -0.361293758469003 1101 8 -0.35343568853212304 1101 9 -0.5374186932833993 1101 10-11 -0.4803209103984827 1101 12-13 -0.48645843769678976 1101 14-15 -0.7130399018046489 1101 16-17 -0.6649609470451239 1101 18-19 -0.7047899346254951 1101 20-21 -0.731797078793285 1101 22-23 -0.793228964628824 1101 24-25 -0.7650649800571543 1101 26-27 -0.7784906702618812 1101 28-29 -0.8401496086191642 1101 30-31 -0.7733411504456811 1101 32-33 -0.7783035740367268 1101 34-35 -0.7277916964660278 1101 36-37 -0.689994646837647 1101 38-39 -0.757514879269479 1101 40-41 -0.7552232413384772 1101 42-43 -0.7392147404289986 1101 44-45 -0.855738054285581 1101 46-47 -0.842889703768634 1101 48-49 -0.7767564770151125 1101 50-51 -0.8654836477039822 1101 52-53 -0.8313178957031582 1101 54-55 -0.8104176217196795 1101 56-57 -0.824319636649939 1101 58-59 -0.8050311816640985 1101 60-61 -0.932200096606195 1101 62-63 -0.9397286294707001 1101 64-65 -0.8978295434319037 1101 66-67 -0.9838934346550658 1101 68-69 -0.962476759152274 1101 70-71 -1.0575257603278843 1101 72-73 -1.0394040155542896 1101 74-75 -1.0519334291689049 1101 76-77 -1.0299288801518642 1101 78-79 -0.8753214218829619 1101 80-81 -0.8024826033282295 1101 82-83 -0.8442440195322831 1101 84-85 -0.7887413815741482 1101 86-87 -0.9253106900166763 1101 88-89 -0.9680956912194603 1101 90-91 -1.0022661406393603 1101 92-93 -1.049974452139601 1101 94-95 -1.09251870499968 1101 96-97 -1.1586521829573684 1101 98-99 -1.2910796263514044 1101 100 -1.2514271721410388 1102 1 -0.39152792765988664 1102 2 -0.34279958044324843 1102 3 -0.3354022118292228 1102 4 -0.19393424575685003 1102 5 -0.27109231023425195 1102 6 -0.3433898637788886 1102 7 -0.34850522472670775 1102 8 -0.38974341972127746 1102 9 -0.46282529738044076 1102 10-11 -0.5121248759763901 1102 12-13 -0.47247573531131337 1102 14-15 -0.6301963908578045 1102 16-17 -0.695299116110462 1102 18-19 -0.6559638529276484 1102 20-21 -0.7083778701309669 1102 22-23 -0.6216376799656587 1102 24-25 -0.6649086359400442 1102 26-27 -0.7586635620912645 1102 28-29 -0.8198120715592623 1102 30-31 -0.7464576143290955 1102 32-33 -0.7356182029165126 1102 34-35 -0.7359061702046645 1102 36-37 -0.8338835140418581 1102 38-39 -0.8577429827160117 1102 40-41 -0.8792657839904834 1102 42-43 -0.7622265799733228 1102 44-45 -0.7983280467356764 1102 46-47 -0.8577890139268831 1102 48-49 -0.8237132363587136 1102 50-51 -0.8807969232417037 1102 52-53 -0.9498107746118549 1102 54-55 -0.927632070650553 1102 56-57 -0.9891671979557373 1102 58-59 -0.9310008502730085 1102 60-61 -0.9958784242274916 1102 62-63 -0.9648172101754753 1102 64-65 -0.8682627644434504 1102 66-67 -1.0525794059442077 1102 68-69 -1.0335058569879507 1102 70-71 -1.0816737195972266 1102 72-73 -1.075390819542399 1102 74-75 -1.2167563862376767 1102 76-77 -0.9459148780513829 1102 78-79 -0.8123216278753027 1102 80-81 -0.8265343444032709 1102 82-83 -0.776200547734156 1102 84-85 -0.8895283410087025 1102 86-87 -0.8198127006225953 1102 88-89 -0.861035160144457 1102 90-91 -0.8820200917272984 1102 92-93 -1.055736504599615 1102 94-95 -1.1689123169692763 1102 96-97 -1.1903020267949636 1102 98-99 -1.1677031062220493 1102 100 -1.2349533816137352 1103 1 -0.3490563933458013 1103 2 -0.38019923103743736 1103 3 -0.39593953435615603 1103 4 -0.27962727485364525 1103 5 -0.3043011235570958 1103 6 -0.3849186778543867 1103 7 -0.45931399362287806 1103 8 -0.4366854150639057 1103 9 -0.559391442592073 1103 10-11 -0.5771432257692624 1103 12-13 -0.5696589056956682 1103 14-15 -0.711450774342623 1103 16-17 -0.7279308800083228 1103 18-19 -0.7556559974768646 1103 20-21 -0.7388116232428672 1103 22-23 -0.7329338693028546 1103 24-25 -0.8687438335903579 1103 26-27 -0.8763270809887302 1103 28-29 -0.8744281087663452 1103 30-31 -0.806559521693849 1103 32-33 -0.6886975016393819 1103 34-35 -0.8351450707493271 1103 36-37 -0.8956239961556633 1103 38-39 -0.8344021014803289 1103 40-41 -0.8716234139064127 1103 42-43 -0.8201348125696555 1103 44-45 -0.8531909317163553 1103 46-47 -0.971117098793755 1103 48-49 -0.9067457551078633 1103 50-51 -1.0451032253388917 1103 52-53 -1.0412656887641916 1103 54-55 -0.9826190483240467 1103 56-57 -0.887665372043486 1103 58-59 -0.8666473385503508 1103 60-61 -1.061546772676465 1103 62-63 -0.9346107344059718 1103 64-65 -0.9255133946427563 1103 66-67 -1.0633501812720851 1103 68-69 -1.1376629595041052 1103 70-71 -1.101047099885946 1103 72-73 -1.0359508347922528 1103 74-75 -1.0572970156288477 1103 76-77 -1.0483489477374484 1103 78-79 -0.9270351287024425 1103 80-81 -0.8537731258735306 1103 82-83 -0.8039732967489925 1103 84-85 -0.9235545340378977 1103 86-87 -0.8830096447482418 1103 88-89 -0.9761939780537823 1103 90-91 -0.9385041355687278 1103 92-93 -1.0889395186112019 1103 94-95 -1.0183008998921288 1103 96-97 -1.1003722417316233 1103 98-99 -1.126728772734829 1103 100 -1.3526798311179213 1104 1 -0.3315332592179203 1104 2 -0.33743583857918935 1104 3 -0.37389131489962324 1104 4 -0.2368640461111582 1104 5 -0.2948518866471588 1104 6 -0.3393421575462128 1104 7 -0.31754808125170086 1104 8 -0.31687393121122653 1104 9 -0.4357484643099454 1104 10-11 -0.4999836803256983 1104 12-13 -0.5729210876760007 1104 14-15 -0.7280687149555689 1104 16-17 -0.6537002822173363 1104 18-19 -0.6325571194173136 1104 20-21 -0.7572750224389324 1104 22-23 -0.7089348492689993 1104 24-25 -0.6939272207049214 1104 26-27 -0.7558353490710275 1104 28-29 -0.8790492770536318 1104 30-31 -0.8072096005830147 1104 32-33 -0.7232205707175368 1104 34-35 -0.734993548178231 1104 36-37 -0.6729170408845562 1104 38-39 -0.7411887526359564 1104 40-41 -0.7959449958197382 1104 42-43 -0.7130246790881998 1104 44-45 -0.7763691864510847 1104 46-47 -0.7921438065091735 1104 48-49 -0.7790692025804233 1104 50-51 -0.628978609416059 1104 52-53 -0.6548609853778444 1104 54-55 -0.6746280076506537 1104 56-57 -0.6719706718790732 1104 58-59 -0.7470305945904414 1104 60-61 -0.743752214195311 1104 62-63 -0.8850619241174584 1104 64-65 -0.8115947048373968 1104 66-67 -0.858088049425163 1104 68-69 -0.8302889362323072 1104 70-71 -0.8791304096684627 1104 72-73 -0.8954641518684952 1104 74-75 -0.8632333598865571 1104 76-77 -0.7499027357853549 1104 78-79 -0.6314993190458011 1104 80-81 -0.6365637093361158 1104 82-83 -0.6653108985037939 1104 84-85 -0.6505162071605461 1104 86-87 -0.7184817758879092 1104 88-89 -0.8283245512655775 1104 90-91 -0.8032520002059229 1104 92-93 -0.796163701946071 1104 94-95 -0.8287064569288063 1104 96-97 -0.8583983612345243 1104 98-99 -0.8856089791505681 1104 100 -1.0108026029464696 1105 1 -0.40544380355734333 1105 2 -0.39213400233796847 1105 3 -0.39684035025830866 1105 4 -0.2386275508242548 1105 5 -0.2667304446114258 1105 6 -0.3751521558422226 1105 7 -0.3712603332960498 1105 8 -0.4064853978280567 1105 9 -0.5618763997870815 1105 10-11 -0.5201053225360823 1105 12-13 -0.5802849866063724 1105 14-15 -0.8107666940171327 1105 16-17 -0.7391019271871215 1105 18-19 -0.7090226911473394 1105 20-21 -0.7388692918722342 1105 22-23 -0.7522703749912978 1105 24-25 -0.7074890160032083 1105 26-27 -0.7489721631338426 1105 28-29 -0.7411808702138458 1105 30-31 -0.6767582686698645 1105 32-33 -0.7913025025826883 1105 34-35 -0.7802773679644517 1105 36-37 -0.7124507776823137 1105 38-39 -0.7620284366253429 1105 40-41 -0.8192784424084891 1105 42-43 -0.7404499872657553 1105 44-45 -0.7590014933415148 1105 46-47 -0.7827036299840344 1105 48-49 -0.8278873098657797 1105 50-51 -0.9188243083734449 1105 52-53 -0.9551998045479095 1105 54-55 -0.8440941773987447 1105 56-57 -0.92765790548318 1105 58-59 -0.9738142973378814 1105 60-61 -0.9092653032290912 1105 62-63 -0.9695072118766248 1105 64-65 -0.9057167525562022 1105 66-67 -0.8915409286381859 1105 68-69 -0.9562602180988407 1105 70-71 -0.9729473495140724 1105 72-73 -0.9242368800926499 1105 74-75 -0.9638899194534005 1105 76-77 -0.8915062680865837 1105 78-79 -0.8385540822884323 1105 80-81 -0.8583277896618711 1105 82-83 -0.8075703786452308 1105 84-85 -0.7933995010682189 1105 86-87 -0.8253562234913865 1105 88-89 -0.8056841614763108 1105 90-91 -0.8815083463907207 1105 92-93 -0.919273724593225 1105 94-95 -0.8958198154760844 1105 96-97 -0.9844997087135319 1105 98-99 -0.9477629298138552 1105 100 -0.9611204870621535 1106 1 -0.43004744113393656 1106 2 -0.4012693703634511 1106 3 -0.4432381494075308 1106 4 -0.30331138454531725 1106 5 -0.36540777714723305 1106 6 -0.3624241944370752 1106 7 -0.4047442522882889 1106 8 -0.39948316283469154 1106 9 -0.5310875238626878 1106 10-11 -0.5728683169977131 1106 12-13 -0.5920015118015911 1106 14-15 -0.7107449274670685 1106 16-17 -0.7447700755294377 1106 18-19 -0.6796287436279087 1106 20-21 -0.6964422562668062 1106 22-23 -0.6882677241377735 1106 24-25 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0.3304926725219204 2311 28-29 0.6604990575779226 2311 30-31 0.8908715453016995 2311 32-33 0.9460966891614504 2311 34-35 0.9798533486696712 2311 36-37 0.9861632475101487 2311 38-39 1.0161059830133468 2311 40-41 1.0050591745051065 2311 42-43 0.9737732241796948 2311 44-45 0.9728624767843499 2311 46-47 0.5545298011765212 2311 48-49 0.8369896428504546 2311 50-51 1.0383987042834093 2311 52-53 0.9140060922428503 2311 54-55 0.8747884254003679 2311 56-57 0.998962713859072 2311 58-59 1.039770117778552 2311 60-61 1.0809079202796994 2311 62-63 1.0858730447157967 2311 64-65 1.1396591183968283 2311 66-67 1.1771947961085587 2311 68-69 1.166569036567978 2311 70-71 1.1391333896836358 2311 72-73 1.1800534726222978 2311 74-75 1.1745151265316736 2311 76-77 1.1545762211906379 2311 78-79 0.8954647187466023 2311 80-81 0.819201696917041 2311 82-83 0.9494996228653676 2311 84-85 1.0216318767468344 2311 86-87 1.0609389755609193 2311 88-89 1.1003302916247328 2311 90-91 1.0872922083798358 2311 92-93 1.156227509662088 2311 94-95 1.1568844612949256 2311 96-97 1.1935663788331645 2311 98-99 1.2002286400457862 2311 100 1.2686218979381607 2312 1 0.2503710983209899 2312 2 0.3714785046344815 2312 3 0.450058585696425 2312 4 0.19131434069667108 2312 5 0.3637761276715139 2312 6 0.43342588120935943 2312 7 0.29821118710518135 2312 8 0.4195009002806529 2312 9 0.607623342599112 2312 10-11 0.6386103731267383 2312 12-13 0.6663131323865699 2312 14-15 0.8489467811046296 2312 16-17 0.8527440534465995 2312 18-19 0.8067678475145144 2312 20-21 0.824448524660994 2312 22-23 0.9091007742731989 2312 24-25 0.8900489127276998 2312 26-27 0.9354561861557968 2312 28-29 0.9174042693706213 2312 30-31 0.9494366163658228 2312 32-33 0.8115369113242963 2312 34-35 0.9096870764502043 2312 36-37 0.9618371478295273 2312 38-39 0.7767457191154463 2312 40-41 0.8988216600314445 2312 42-43 0.9896481335204541 2312 44-45 0.7422810488854807 2312 46-47 0.781487245413139 2312 48-49 0.9516719856123501 2312 50-51 0.7717189954108861 2312 52-53 0.9621684136282553 2312 54-55 0.8490698316682668 2312 56-57 0.8994912412986054 2312 58-59 1.0286158507505263 2312 60-61 1.0936232757275235 2312 62-63 1.094043509527289 2312 64-65 1.114394616100121 2312 66-67 1.1038928276897835 2312 68-69 1.138555332708755 2312 70-71 1.0567002866565645 2312 72-73 1.0745300984445905 2312 74-75 1.060925466137995 2312 76-77 1.0245350905268253 2312 78-79 0.9404340854679916 2312 80-81 0.9038320935273347 2312 82-83 0.9083403298165464 2312 84-85 0.9363740227940873 2312 86-87 0.9497152031187817 2312 88-89 0.95559679796769 2312 90-91 1.0634114523136162 2312 92-93 1.1151819382862271 2312 94-95 1.1210470244382869 2312 96-97 0.8271980923881479 2312 98-99 0.6041211022870066 2312 100 0.8085962588700717 2313 1 0.01864700719031731 2313 2 0.2020205290129411 2313 3 0.2590564835419116 2313 4 0.19414079047528077 2313 5 0.29722708591958735 2313 6 0.21472329840723603 2313 7 0.23574270057272884 2313 8 0.39432215419794403 2313 9 0.587371229324738 2313 10-11 0.38015739670242965 2313 12-13 0.44995348445612393 2313 14-15 0.443770454758166 2313 16-17 0.7027621530460735 2313 18-19 0.6697295472678562 2313 20-21 0.6516787858078175 2313 22-23 0.6178243834626898 2313 24-25 0.4561475051509376 2313 26-27 0.6950693112811308 2313 28-29 0.5686099766277763 2313 30-31 0.7566155961211791 2313 32-33 0.8727413831186297 2313 34-35 0.9100554498195166 2313 36-37 0.9345188928149142 2313 38-39 0.9347370640965806 2313 40-41 0.9230315258730428 2313 42-43 0.9779601260667263 2313 44-45 0.8130673268918684 2313 46-47 0.8527860052948526 2313 48-49 0.9606131635396764 2313 50-51 0.9037188589980829 2313 52-53 0.9429965521033026 2313 54-55 0.9050117748769608 2313 56-57 0.6963095579134091 2313 58-59 0.7871539987087459 2313 60-61 0.6223562601844179 2313 62-63 0.9668112284748602 2313 64-65 1.0502880864162591 2313 66-67 1.0029685894380336 2313 68-69 1.0785064267816367 2313 70-71 1.0292725797357463 2313 72-73 1.031370923863804 2313 74-75 1.0878917595164168 2313 76-77 1.0885572441875553 2313 78-79 0.9187259626596571 2313 80-81 0.8671459555239238 2313 82-83 0.905877532223748 2313 84-85 0.8717523825906106 2313 86-87 0.9315838523919844 2313 88-89 0.9130385107203729 2313 90-91 0.8408953983873957 2313 92-93 0.9760491100538147 2313 94-95 1.0333094667628941 2313 96-97 1.1619275817165793 2313 98-99 1.155885541724114 2313 100 1.2734372767337163 2314 1 -0.3496650489147619 2314 2 0.05019906772375293 2314 3 0.29705696795580394 2314 4 0.245415790352979 2314 5 0.347583349226376 2314 6 0.4045696713098863 2314 7 0.4640396058508429 2314 8 0.46953321453353425 2314 9 0.44902753060964073 2314 10-11 0.5872133556288617 2314 12-13 0.4555127042756624 2314 14-15 0.7442667078940985 2314 16-17 0.8158678226708744 2314 18-19 0.8342055568370057 2314 20-21 0.8843063784742782 2314 22-23 0.8991811748144158 2314 24-25 0.8336437686562874 2314 26-27 0.7334773776548218 2314 28-29 0.8003901928334543 2314 30-31 0.8804881226698242 2314 32-33 0.8910472335886368 2314 34-35 0.9285401478078441 2314 36-37 0.8658520747040299 2314 38-39 0.6482038653513698 2314 40-41 0.6716007153581387 2314 42-43 0.8790576085600108 2314 44-45 0.9081720995348803 2314 46-47 0.865522868378406 2314 48-49 0.8770231651615674 2314 50-51 0.8765490602663917 2314 52-53 0.9201124403810184 2314 54-55 0.8492928191724332 2314 56-57 0.842154792190513 2314 58-59 0.9237269242302091 2314 60-61 1.002925668586684 2314 62-63 0.9203950382241644 2314 64-65 1.0141152744481516 2314 66-67 0.7170186618621486 2314 68-69 0.7034499568275905 2314 70-71 0.7887798760530131 2314 72-73 0.9800700225409997 2314 74-75 1.03683056941464 2314 76-77 0.9020683633615505 2314 78-79 0.7802516303291824 2314 80-81 0.7911658083438624 2314 82-83 0.8641643674661452 2314 84-85 0.8901356613199845 2314 86-87 0.9797049496374903 2314 88-89 0.8983013172953953 2314 90-91 1.0050448708509947 2314 92-93 0.9407790774670275 2314 94-95 0.8773685106613236 2314 96-97 0.9624081150882375 2314 98-99 1.0379214545001503 2314 100 0.9938107195839478 2315 1 0.39426724455892526 2315 2 0.44161772660904575 2315 3 0.4307098348068905 2315 4 0.2984472332596866 2315 5 0.3753439972085175 2315 6 0.41766191590669877 2315 7 0.3785875033813966 2315 8 0.24225856752101294 2315 9 0.5013556890583715 2315 10-11 0.5225554868774509 2315 12-13 0.3888104441892466 2315 14-15 0.6061179197403774 2315 16-17 0.7663612227983307 2315 18-19 0.733617531043933 2315 20-21 0.48798496971988925 2315 22-23 0.7918969383801979 2315 24-25 0.8347629606356008 2315 26-27 0.7811168243636146 2315 28-29 0.7559008225216886 2315 30-31 0.8384093644715449 2315 32-33 0.7856941653816705 2315 34-35 0.837304816358099 2315 36-37 0.8394653897327942 2315 38-39 0.8408854802969401 2315 40-41 0.878460328691915 2315 42-43 0.966273356491925 2315 44-45 0.9602201812012225 2315 46-47 0.9722677474398651 2315 48-49 0.8772238106083563 2315 50-51 0.9718595967520116 2315 52-53 1.0118491216194911 2315 54-55 0.7879921074976934 2315 56-57 0.8505441055219052 2315 58-59 0.8460488495874614 2315 60-61 0.9785402159163041 2315 62-63 1.0374667524807677 2315 64-65 1.0525582403230516 2315 66-67 1.050217442799486 2315 68-69 1.1665972278330088 2315 70-71 1.0337941807651063 2315 72-73 1.1543099954309852 2315 74-75 0.586071475223001 2315 76-77 0.567366773322675 2315 78-79 0.816435301818494 2315 80-81 0.8466871315069397 2315 82-83 0.8529877605895919 2315 84-85 0.8404375112748106 2315 86-87 0.8758446378606344 2315 88-89 0.8918449836304383 2315 90-91 0.9051698938801991 2315 92-93 0.9702967235282642 2315 94-95 1.0325967114161294 2315 96-97 1.073403266893692 2315 98-99 1.109131494560046 2315 100 1.1103091813402663 2316 1 0.3314915093680426 2316 2 0.13539338324142847 2316 3 0.23753030433560696 2316 4 0.04062159492568185 2316 5 0.30421915917347064 2316 6 0.38942661253910416 2316 7 0.46385927909867775 2316 8 0.4947922771422597 2316 9 0.6724595891737195 2316 10-11 0.670745810387146 2316 12-13 0.4450975715839647 2316 14-15 0.4990241769937427 2316 16-17 0.7781944509795693 2316 18-19 0.7528620989619839 2316 20-21 0.7350014978586614 2316 22-23 0.7784272786415443 2316 24-25 0.5125854123622702 2316 26-27 0.7754927233843105 2316 28-29 0.9699124929177358 2316 30-31 0.525174765751828 2316 32-33 0.6803051881888109 2316 34-35 0.8324550186775994 2316 36-37 0.8829422300745193 2316 38-39 0.8257917277717794 2316 40-41 0.7945282924260368 2316 42-43 0.8344593734897643 2316 44-45 0.7040381100993756 2316 46-47 0.7855108858444098 2316 48-49 0.8657937976035868 2316 50-51 0.9241130626582503 2316 52-53 0.9241548999501461 2316 54-55 0.8545228924172932 2316 56-57 0.9393859319930016 2316 58-59 0.75891444803041 2316 60-61 0.9352827315019709 2316 62-63 0.938915750344627 2316 64-65 1.02753248747468 2316 66-67 1.0066150509213116 2316 68-69 1.0791180014009356 2316 70-71 0.9912556816525395 2316 72-73 1.0324786892569549 2316 74-75 1.0260022254817187 2316 76-77 0.8728356436442795 2316 78-79 0.6202298323105921 2316 80-81 0.5810674037712502 2316 82-83 0.5813016852179658 2316 84-85 0.7141135545484438 2316 86-87 0.7907885676445368 2316 88-89 0.7412253758137233 2316 90-91 0.6284108327790712 2316 92-93 0.44614930286655863 2316 94-95 0.4527599908506694 2316 96-97 0.6518330041839171 2316 98-99 0.8703704796054552 2316 100 0.46848077436445834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 28.0 13 153.0 14 665.0 15 1909.0 16 4417.0 17 9040.0 18 16031.0 19 23866.0 20 34456.0 21 49154.0 22 62206.0 23 64713.0 24 64105.0 25 67097.0 26 78060.0 27 95882.0 28 119118.0 29 147653.0 30 182130.0 31 229442.0 32 290841.0 33 385423.0 34 557953.0 35 772326.0 36 1192300.0 37 1783694.0 38 1513436.0 39 233788.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.214542155305594 20.822459193532797 13.92838933100048 23.034609320161127 2 15.574375288380013 20.168918944320506 41.03020054581987 23.22650522147961 3 18.92257844124291 26.20669725844438 29.413056919603974 25.45766738070873 4 12.621090981844047 16.618990987146685 38.661977948802104 32.097940082207174 5 12.924011036699357 38.478458812003424 34.298618457674145 14.29891169362307 6 30.316624648712104 37.46230852883023 17.868620770979966 14.352446051477695 7 27.611716413645976 32.2846375018672 22.275637330435366 17.828008754051456 8 26.614934331438327 35.67947155373198 19.472796851899055 18.23279726293064 9 26.806751082773555 15.680872124311604 21.1133386121599 36.39903818075494 10-11 25.873314699812678 26.33669236294663 27.00763569996757 20.78235723727312 12-13 25.83023065015305 23.859001862299138 27.918340294346272 22.392427193201534 14-15 23.16376711469589 25.089584332456838 25.910740107251822 25.835908445595447 16-17 21.93161139799173 27.927528387897738 26.868711463318384 23.272148750792145 18-19 21.53168289113603 27.53047586179414 29.27303675399334 21.6648044930765 20-21 22.85426760490486 26.681667650594537 28.94787429599867 21.516190448501934 22-23 22.934221662818256 26.402783134972996 28.65609463350644 22.0069005687023 24-25 22.282224543199384 26.413817306571097 29.30543473949753 21.99852341073198 26-27 22.300795494069554 26.631040415564968 28.55623142869191 22.51193266167357 28-29 21.628083116706048 27.350821663196285 28.9095105309432 22.11158468915447 30-31 22.72236114680041 26.734648750565416 28.820021229989738 21.722968872644437 32-33 22.902767975661256 26.654447913255247 28.30058308858853 22.142201022494966 34-35 22.694383032996097 26.832910547501125 28.840691478110454 21.632014941392324 36-37 22.444923893803757 26.577328048345343 28.66962872856995 22.308119329280945 38-39 22.605609506712238 26.71175438173186 28.898350839997107 21.784285271558797 40-41 22.302407733298544 26.133987332310006 29.318040524427282 22.245564409964175 42-43 22.389521393458278 26.230464080073023 28.539260313015824 22.840754213452875 44-45 22.23149862887357 26.877950666519528 28.649614830686986 22.24093587391991 46-47 22.171453853652363 26.73413964587966 28.146384612901983 22.948021887565993 48-49 22.850780958584828 26.729203673570336 28.082703141623345 22.337312226221492 50-51 22.63743129257839 26.680452799258557 28.003351283504614 22.67876462465844 52-53 22.24790203221419 27.10036469340701 27.696734102173558 22.954999172205245 54-55 22.148019418460095 26.987639032606214 27.817474846970686 23.046866701963005 56-57 22.862648305671495 26.64085343564389 27.433345860661934 23.06315239802268 58-59 22.003897843465655 27.212272429456075 27.990675604477794 22.793154122600473 60-61 22.467228575306322 27.36372763058348 27.617694344909243 22.551349449200956 62-63 22.619086109024597 26.865870557885184 27.32045508830156 23.194588244788665 64-65 22.085721912896133 26.940369433360473 27.626564186760156 23.347344466983245 66-67 22.36412769699659 26.759301978791733 27.5738389442348 23.302731379976876 68-69 21.873393259585054 26.769394816412873 27.73891385554817 23.618298068453907 70-71 21.948168355612438 26.414042807578543 27.943278052665416 23.694510784143603 72-73 22.29724443802543 26.792152104533628 27.801034163191375 23.109569294249567 74-75 22.52811371269238 26.950027434586033 27.487681388243956 23.034177464477633 76-77 22.31766365490058 26.71977438360666 27.204347462834072 23.758214498658685 78-79 22.103918593924224 26.74679567822719 27.487867252834462 23.66141847501412 80-81 21.810684401026137 27.119594768766635 27.72827872843048 23.341442101776742 82-83 22.54305709394256 26.457281948411033 28.30049018541649 22.69917077222992 84-85 22.748042121232544 26.834271850898734 27.31008693498918 23.107599092879543 86-87 21.710839821899295 27.40492908453025 27.557022236758634 23.327208856811822 88-89 22.15867750976569 26.40968229385467 27.73853041313712 23.693109783242523 90-91 22.293669540273118 26.321169402360066 27.81662955551878 23.568531501848035 92-93 22.487293175622955 26.634800150837123 27.5049719085911 23.372934764948823 94-95 21.597785906176114 26.596241204128145 27.98181700826619 23.824155881429547 96-97 21.68961044657347 26.522109365699 27.76188178679516 24.026398400932372 98-99 21.276884698997662 26.894977077375387 27.666108809266916 24.16202941436003 100 21.644371314698287 26.525610551342073 27.285943185274007 24.544074948685633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2232.0 1 1586.5 2 1360.5 3 1855.5 4 1477.0 5 1147.5 6 1729.0 7 3255.5 8 5118.5 9 6046.5 10 5348.0 11 4445.5 12 4702.5 13 5451.0 14 6264.0 15 7360.0 16 8593.5 17 9716.0 18 10913.0 19 12021.0 20 12996.5 21 13894.5 22 15246.5 23 17268.0 24 19295.5 25 21774.5 26 25767.0 27 30563.0 28 37194.0 29 48348.5 30 64251.5 31 81686.5 32 104575.5 33 129729.0 34 151437.5 35 174342.5 36 194731.0 37 225331.0 38 240845.5 39 231811.0 40 222436.0 41 215791.5 42 221585.0 43 239591.5 44 268844.0 45 313798.0 46 349353.0 47 364154.0 48 373720.0 49 375016.0 50 367354.5 51 365662.0 52 356656.5 53 329304.5 54 303290.5 55 267962.5 56 233663.5 57 193883.5 58 155290.0 59 139125.5 60 111689.5 61 78236.5 62 58414.5 63 46835.5 64 33169.0 65 17963.0 66 10692.0 67 7183.5 68 5179.0 69 3734.5 70 2728.0 71 1972.0 72 1462.5 73 1163.0 74 869.5 75 599.0 76 384.5 77 203.0 78 109.0 79 77.0 80 58.0 81 36.0 82 26.5 83 18.5 84 12.5 85 9.0 86 6.5 87 5.0 88 2.5 89 2.0 90 1.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042970344822919324 2 0.0 3 0.0 4 0.0345742727811124 5 0.0 6 0.0 7 9.273273598413618E-4 8 0.0 9 0.010977550908392338 10-11 4.76195130729348E-4 12-13 1.2531450808667052E-5 14-15 1.3784595889533758E-4 16-17 3.132862702166763E-4 18-19 0.0 20-21 1.2531450808667052E-5 22-23 0.0 24-25 5.701810117943509E-4 26-27 4.8246085613368157E-4 28-29 1.2531450808667051E-4 30-31 0.0021616752644950665 32-33 0.015075335322826464 34-35 0.02612180921066647 36-37 0.031121858083324624 38-39 0.022174402205936347 40-41 0.0011654249252060359 42-43 7.518870485200231E-5 44-45 0.011259508551587347 46-47 0.017788394422902882 48-49 0.0034273517961704385 50-51 0.011146725494309343 52-53 0.011165522670522343 54-55 0.010194335232850647 56-57 4.386007783033468E-4 58-59 0.007995065615929579 60-61 0.004172973119286129 62-63 0.0013533966873360416 64-65 0.03525723685018475 66-67 0.059869006238406845 68-69 0.05928002805039949 70-71 0.05185514344626426 72-73 0.045338789025757394 74-75 0.0346494614859644 76-77 0.031171983886559295 78-79 0.015106663949848132 80-81 0.0010401104171193652 82-83 0.03812693908536951 84-85 0.05181128336843393 86-87 0.056974241101604754 88-89 0.06982524390589281 90-91 0.0486470920392455 92-93 0.03892268621171986 94-95 0.012995114488587733 96-97 0.003408554619957438 98-99 0.02228091953781002 100 0.032882526921942344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7979922.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.17895412989551 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30881082631092 43.23208896511026 2 15.32050922715359 17.82662407146419 3 5.0120789126757135 8.747925274679307 4 2.089681963226411 4.863020443384776 5 1.0643928251473398 3.0962630675218485 6 0.5729947932471476 2.000174267579686 7 0.3706412714605136 1.5094465071663157 8 0.238344896368537 1.109332543433585 9 0.16163568284666513 0.8463415480281422 >10 0.7701760631821745 7.910555036440457 >50 0.05329054322165446 2.1529616394892472 >100 0.03380133984799746 3.714311265272475 >500 0.0027312692463013774 1.0533753211581032 >1k 7.765057617949451E-4 0.7601923874712425 >5k 5.355212129833652E-5 0.18045592318463624 >10k+ 8.032818194750479E-5 0.9969317386156753 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26693 0.3345020164357496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23141 0.28999030316336427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14162 0.1774704063523428 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.2531450808667052E-5 0.0 0.0 0.0 6 2.5062901617334105E-5 1.2531450808667052E-5 0.0 0.0 0.0 7 2.5062901617334105E-5 1.2531450808667052E-5 0.0 0.0 0.0 8 2.5062901617334105E-5 1.2531450808667052E-5 0.0 0.0 0.0 9 2.5062901617334105E-5 1.2531450808667052E-5 0.0 0.0 0.0 10-11 2.5062901617334105E-5 1.2531450808667052E-5 0.0 0.0 0.0 12-13 2.5062901617334105E-5 1.2531450808667052E-5 0.0 1.2531450808667052E-5 0.0 14-15 2.5062901617334105E-5 1.8797176213000578E-5 0.0 1.2531450808667052E-5 1.8797176213000578E-5 16-17 3.7594352426001156E-5 2.5062901617334105E-5 0.0 1.8797176213000578E-5 2.5062901617334105E-5 18-19 3.7594352426001156E-5 2.5062901617334105E-5 0.0 4.386007783033468E-5 6.265725404333526E-5 20-21 3.7594352426001156E-5 2.5062901617334105E-5 0.0 1.0651733187366994E-4 6.265725404333526E-5 22-23 3.7594352426001156E-5 2.5062901617334105E-5 0.0 2.5689474157767456E-4 1.002516064693364E-4 24-25 3.7594352426001156E-5 4.386007783033468E-5 0.0 7.706842247330237E-4 1.2531450808667051E-4 26-27 3.7594352426001156E-5 5.012580323466821E-5 0.0 0.001478711195422712 1.3784595889533758E-4 28-29 3.7594352426001156E-5 5.012580323466821E-5 0.0 0.00412284731605146 1.5037740970400462E-4 30-31 3.7594352426001156E-5 5.012580323466821E-5 0.0 0.014398636979158443 1.5037740970400462E-4 32-33 3.7594352426001156E-5 5.012580323466821E-5 0.0 0.039329958363001544 1.8170603672567227E-4 34-35 3.7594352426001156E-5 5.012580323466821E-5 0.0 0.07667368177282936 2.0050321293867284E-4 36-37 3.7594352426001156E-5 6.265725404333526E-5 0.0 0.13721938635490422 2.0050321293867284E-4 38-39 3.7594352426001156E-5 8.772015566066937E-5 0.0 0.2302466114330441 2.1930038915167341E-4 40-41 3.7594352426001156E-5 1.1278305727800347E-4 0.0 0.3347401140011143 2.5062901617334103E-4 42-43 3.7594352426001156E-5 2.2556611455600695E-4 0.0 0.43484134306074673 2.631604669820081E-4 44-45 3.7594352426001156E-5 2.38097565364674E-4 0.0 0.5511144093889639 3.132862702166763E-4 46-47 3.7594352426001156E-5 2.38097565364674E-4 0.0 0.6856270023691962 3.132862702166763E-4 48-49 3.7594352426001156E-5 2.443632907690075E-4 0.0 0.834055771472453 3.383491718340104E-4 50-51 5.012580323466821E-5 2.5062901617334103E-4 0.0 0.9927853931404342 3.383491718340104E-4 52-53 5.012580323466821E-5 2.5062901617334103E-4 0.0 1.1493909338963464 3.759435242600116E-4 54-55 5.012580323466821E-5 2.5689474157767456E-4 0.0 1.3198625249720486 3.9474070047301215E-4 56-57 5.012580323466821E-5 2.631604669820081E-4 0.0 1.5157291011115146 4.010064258773457E-4 58-59 5.012580323466821E-5 2.631604669820081E-4 0.0 1.7364706071061846 4.010064258773457E-4 60-61 6.892297944766879E-5 2.7569191779067516E-4 0.0 1.9587472158249164 4.010064258773457E-4 62-63 8.772015566066937E-5 2.7569191779067516E-4 0.0 2.1971768646360204 4.448665037076803E-4 64-65 8.772015566066937E-5 2.7569191779067516E-4 0.0 2.433820781706889 4.636636799206809E-4 66-67 8.772015566066937E-5 2.882233685993422E-4 0.0 2.6932406106225097 4.636636799206809E-4 68-69 8.772015566066937E-5 2.882233685993422E-4 0.0 2.984014129461416 4.636636799206809E-4 70-71 1.0025160646933642E-4 2.882233685993422E-4 0.0 3.296604904158211 4.76195130729348E-4 72-73 1.1278305727800347E-4 2.882233685993422E-4 0.0 3.602785841766373 4.88726581538015E-4 74-75 1.1278305727800347E-4 2.882233685993422E-4 0.0 3.900927853680775 4.88726581538015E-4 76-77 1.19048782682337E-4 3.132862702166763E-4 0.0 4.232347133217592 5.137894831553491E-4 78-79 1.2531450808667051E-4 3.132862702166763E-4 0.0 4.5993181386985995 5.137894831553491E-4 80-81 1.2531450808667051E-4 3.1955199562100985E-4 0.0 4.9810386617814055 5.263209339640162E-4 82-83 1.2531450808667051E-4 3.258177210253434E-4 0.0 5.367496073269889 5.513838355813503E-4 84-85 1.2531450808667051E-4 3.258177210253434E-4 0.0 5.748765464123585 5.513838355813503E-4 86-87 1.2531450808667051E-4 3.258177210253434E-4 0.0 6.142704151744842 5.576495609856838E-4 88 1.2531450808667051E-4 3.258177210253434E-4 0.0 6.462531839283642 5.639152863900174E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15730 0.0 49.077255 1 GTATCAA 27485 0.0 40.044373 1 TACCTGG 4765 0.0 35.70276 2 GGGCACC 6980 0.0 34.215885 94 GTACTAG 5895 0.0 33.895412 1 CAACGCA 32970 0.0 32.206398 5 ATCAACG 33125 0.0 32.05236 3 GTGGTAT 5320 0.0 31.814617 1 TATCAAC 34975 0.0 31.388546 2 CTGTGCG 8675 0.0 30.383886 9 AACGCAG 36620 0.0 29.47103 6 TCAACGC 37035 0.0 28.972982 4 ACCTGGG 5820 0.0 28.749258 3 CTAGTAC 9915 0.0 27.3516 3 ACTGTGC 10620 0.0 26.9428 8 GTACATG 38405 0.0 26.491425 1 GTACTGT 10325 0.0 26.393114 6 CGCAGAG 40560 0.0 26.028856 8 ACGCAGA 41010 0.0 25.869429 7 TACATGG 38855 0.0 25.786737 2 >>END_MODULE