FastQCFastQC Report
Mon 6 Jun 2016
ERR1211228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7640685
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320120.418967671092317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253690.332025204546451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163840.214431035960781No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC98920.1294648320144071No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159450.049.0573731
GTATCAA288200.040.7776641
GTACTAG78600.039.639961
ACCTGGG34350.039.413563
GCATCGT76850.037.73978
TACCTGG37850.036.6825562
GGGCACC82350.035.78362394
TAGGCAT98600.034.803285
CAACGCA337150.033.26765
ATCAACG338900.033.1101233
GTGGTAT51900.033.0089571
CTAGGCA109150.031.9994
TATCAAC369150.031.61932
CTGTGCG102750.031.0629169
AACGCAG388100.029.8451716
ACTGTGC110800.029.6971958
TAGTACT111200.029.4645184
CCTGGGG38750.029.3572274
GTACATG419800.029.0708581
TCTAACG39500.028.9542732
Produced by FastQC (version 0.11.5)