##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211228_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7640685 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.449550662015252 31.0 31.0 34.0 29.0 34.0 2 31.473191736081255 31.0 31.0 34.0 28.0 34.0 3 31.532464301302827 31.0 31.0 34.0 28.0 34.0 4 35.136791269369176 37.0 35.0 37.0 32.0 37.0 5 35.201547897865176 37.0 35.0 37.0 32.0 37.0 6 35.26323032555327 37.0 35.0 37.0 32.0 37.0 7 35.17435216868645 37.0 35.0 37.0 32.0 37.0 8 35.1894429622475 37.0 35.0 37.0 32.0 37.0 9 36.73802911649937 39.0 37.0 39.0 32.0 39.0 10-11 36.69218041314359 39.0 37.0 39.0 32.0 39.0 12-13 36.567175390688135 39.0 37.0 39.0 32.0 39.0 14-15 37.72064625095787 40.0 37.0 41.0 32.0 41.0 16-17 37.69933841010328 40.0 37.0 41.0 32.0 41.0 18-19 37.73086967987818 40.0 37.0 41.0 32.0 41.0 20-21 37.70125512306815 40.0 37.0 41.0 32.0 41.0 22-23 37.59295711052085 40.0 37.0 41.0 32.0 41.0 24-25 37.486775989325565 40.0 37.0 41.0 31.5 41.0 26-27 37.32436901926987 40.0 36.0 41.0 31.0 41.0 28-29 37.206433323713775 40.0 36.0 41.0 31.0 41.0 30-31 37.043015253213554 39.0 36.0 41.0 30.5 41.0 32-33 36.847848524052495 39.0 35.0 41.0 30.0 41.0 34-35 36.705806482010445 39.0 35.0 41.0 30.0 41.0 36-37 36.60085005467442 39.0 35.0 41.0 30.0 41.0 38-39 36.45109509160501 39.0 35.0 41.0 30.0 41.0 40-41 36.27240679336997 39.0 35.0 40.0 29.0 41.0 42-43 36.05595460354667 38.5 35.0 40.0 28.0 41.0 44-45 35.79634600824403 38.0 35.0 40.0 27.5 41.0 46-47 35.5144759272238 38.0 34.0 40.0 26.5 41.0 48-49 35.264364909690684 38.0 34.0 40.0 26.0 41.0 50-51 34.552691676204425 37.5 33.5 39.5 25.0 40.5 52-53 34.64173146779379 37.5 33.5 39.5 25.5 40.5 54-55 35.10172065724474 38.0 34.0 40.0 26.0 41.0 56-57 35.022931582704956 37.5 34.0 40.0 26.0 41.0 58-59 34.77707417594103 37.0 34.0 40.0 26.0 41.0 60-61 34.546474759789206 36.5 33.0 40.0 26.0 41.0 62-63 34.22582340457694 36.0 33.0 39.5 25.0 41.0 64-65 33.976563226988155 35.5 33.0 39.0 25.0 41.0 66-67 33.814051161643235 35.0 33.0 39.0 25.0 41.0 68-69 33.534975725344 35.0 33.0 39.0 24.0 41.0 70-71 33.28521081552243 35.0 32.5 38.0 24.0 40.5 72-73 32.990289352329015 35.0 32.0 37.0 23.5 40.0 74-75 32.678458868543856 35.0 32.0 37.0 23.0 39.5 76-77 32.38658693559543 35.0 32.0 36.0 23.0 39.0 78-79 32.07652782440318 35.0 31.0 36.0 22.0 39.0 80-81 31.8184993230319 35.0 31.0 36.0 20.5 38.0 82-83 31.54272804074504 35.0 31.0 35.0 20.0 37.0 84-85 31.313438651115703 34.5 31.0 35.0 20.0 37.0 86-87 31.100808108173545 34.0 31.0 35.0 20.0 36.5 88-89 30.915924946519848 34.0 30.0 35.0 19.0 36.0 90-91 30.688476491309352 34.0 30.0 35.0 19.0 36.0 92-93 30.49075763495027 34.0 30.0 35.0 18.0 35.5 94-95 30.33159016501793 34.0 29.0 35.0 18.0 35.0 96-97 30.149087745405026 34.0 29.0 35.0 17.5 35.0 98-99 29.92220586243249 34.0 29.0 35.0 17.0 35.0 100 28.75894582750107 33.0 26.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.46051466029173227 1101 2 -0.4383946569857322 1101 3 -0.38941006061890704 1101 4 -0.37102217553768213 1101 5 -0.317716481902103 1101 6 -0.276471727866344 1101 7 -0.39599786678849114 1101 8 -0.3614803510966951 1101 9 -0.5201423832628649 1101 10-11 -0.5011655952123562 1101 12-13 -0.4715339303511641 1101 14-15 -0.7022109562761187 1101 16-17 -0.6782711069423755 1101 18-19 -0.624796395530737 1101 20-21 -0.7541256184310541 1101 22-23 -0.8011356353005681 1101 24-25 -0.8523191375891059 1101 26-27 -0.8079398376185409 1101 28-29 -0.8684089950991591 1101 30-31 -0.8502019760370914 1101 32-33 -0.8847942850622559 1101 34-35 -0.8563623799667042 1101 36-37 -0.8717564360431638 1101 38-39 -0.8584444528904811 1101 40-41 -0.9365578652415891 1101 42-43 -1.016127882760614 1101 44-45 -0.9077622943675223 1101 46-47 -1.1976744620519995 1101 48-49 -1.011514528860701 1101 50-51 -1.128972138007164 1101 52-53 -0.9785475695656416 1101 54-55 -0.8285689993689545 1101 56-57 -0.8327941290490841 1101 58-59 -0.811279337801146 1101 60-61 -0.6707511441270597 1101 62-63 -0.7718370899800888 1101 64-65 -0.7321539013010039 1101 66-67 -0.7613491770293308 1101 68-69 -0.86815780566684 1101 70-71 -0.8729167100111113 1101 72-73 -0.8345015320362776 1101 74-75 -0.8834551772687753 1101 76-77 -0.8918783147335283 1101 78-79 -0.9451084503435503 1101 80-81 -0.9076669799010411 1101 82-83 -0.8679174204909863 1101 84-85 -1.0608907899940867 1101 86-87 -1.1654260113647759 1101 88-89 -1.2025005146667453 1101 90-91 -1.1794068326645153 1101 92-93 -1.4200108093607717 1101 94-95 -1.4537689377591256 1101 96-97 -1.4051970515059793 1101 98-99 -1.480747127669808 1101 100 -1.684669256330693 1102 1 -0.41293741181277355 1102 2 -0.37359222899810973 1102 3 -0.4018165109206464 1102 4 -0.2965439457954844 1102 5 -0.2859452936785516 1102 6 -0.31258165733059684 1102 7 -0.37641416656965276 1102 8 -0.31799538658049187 1102 9 -0.5348175747452473 1102 10-11 -0.547472297090053 1102 12-13 -0.571484316769947 1102 14-15 -0.7036071630452696 1102 16-17 -0.7588689546485696 1102 18-19 -0.6749052604841026 1102 20-21 -0.7739866168588065 1102 22-23 -0.7433699377187111 1102 24-25 -0.7536637169723193 1102 26-27 -0.7912313995709965 1102 28-29 -0.8387831219854363 1102 30-31 -0.7564709002682193 1102 32-33 -0.8300381240823214 1102 34-35 -0.8984316439177533 1102 36-37 -1.0009301320625212 1102 38-39 -1.0566890635780766 1102 40-41 -1.0852051360800132 1102 42-43 -1.0103706873002096 1102 44-45 -1.11965796564958 1102 46-47 -1.0731280798792042 1102 48-49 -1.0723255813359387 1102 50-51 -1.0519432136405342 1102 52-53 -1.040017743029047 1102 54-55 -0.7805705560462428 1102 56-57 -0.8017598811304367 1102 58-59 -0.7897219634758059 1102 60-61 -0.6509809539526117 1102 62-63 -0.7860701484529713 1102 64-65 -0.8177390330461236 1102 66-67 -0.8388707769554031 1102 68-69 -0.8897369182764123 1102 70-71 -0.868750368327639 1102 72-73 -0.8740892336788662 1102 74-75 -0.9014528036393337 1102 76-77 -0.9247871363682663 1102 78-79 -0.9638462071018346 1102 80-81 -1.0548185798896768 1102 82-83 -1.1845776872488294 1102 84-85 -1.194002908229237 1102 86-87 -1.1557914520815764 1102 88-89 -1.13472293439008 1102 90-91 -1.155137991853536 1102 92-93 -1.2053085094482192 1102 94-95 -1.2316526359849362 1102 96-97 -1.3111537632666987 1102 98-99 -1.4269623105395937 1102 100 -1.4605596827425167 1103 1 -0.5272702143678458 1103 2 -0.4389454010220284 1103 3 -0.5122221222094687 1103 4 -0.4257091436898861 1103 5 -0.3954917346194904 1103 6 -0.4112776455511522 1103 7 -0.38406424506433723 1103 8 -0.42285020581339694 1103 9 -0.5526660857377834 1103 10-11 -0.5433861344317705 1103 12-13 -0.5267259766278443 1103 14-15 -0.7391202131141412 1103 16-17 -0.742487202500449 1103 18-19 -0.7031741066613861 1103 20-21 -0.8144830330817854 1103 22-23 -0.824284738225586 1103 24-25 -0.7511265220892867 1103 26-27 -0.8270869527259208 1103 28-29 -0.8831186850406425 1103 30-31 -0.8390188124111759 1103 32-33 -0.90365041685439 1103 34-35 -0.9671661317170006 1103 36-37 -0.9121886318312349 1103 38-39 -0.9113577712102767 1103 40-41 -0.9216188029021808 1103 42-43 -1.0162448788475515 1103 44-45 -1.0045580094178987 1103 46-47 -1.085184127082158 1103 48-49 -1.1499865359800339 1103 50-51 -1.2545838464007275 1103 52-53 -1.0528338798784276 1103 54-55 -0.8221151514939464 1103 56-57 -0.7274677317618625 1103 58-59 -0.7192046270762873 1103 60-61 -0.7932203203144326 1103 62-63 -0.7774904026368503 1103 64-65 -0.6776301269953535 1103 66-67 -0.831871725623941 1103 68-69 -0.8121239068979591 1103 70-71 -0.8177366042388883 1103 72-73 -0.9173377858188587 1103 74-75 -0.9331721111858329 1103 76-77 -0.8550748434651574 1103 78-79 -0.9038205218786217 1103 80-81 -0.9632654830352294 1103 82-83 -0.8221748391629049 1103 84-85 -0.9553185017420915 1103 86-87 -1.1154891635978892 1103 88-89 -1.144323801124056 1103 90-91 -1.2421382803116714 1103 92-93 -1.3806431642943835 1103 94-95 -1.3785145706004336 1103 96-97 -1.4120577420888623 1103 98-99 -1.3529032332157769 1103 100 -1.5791623332157805 1104 1 -0.5650136195096671 1104 2 -0.5779015601661115 1104 3 -0.5455814417594276 1104 4 -0.4025965809286518 1104 5 -0.4019459550658553 1104 6 -0.45443018163064863 1104 7 -0.4852033121235664 1104 8 -0.42053871952229116 1104 9 -0.5772343969020497 1104 10-11 -0.598663663939405 1104 12-13 -0.6030005471570092 1104 14-15 -0.7841889383053768 1104 16-17 -0.7731419565815756 1104 18-19 -0.8883853343499268 1104 20-21 -0.804769212795776 1104 22-23 -0.8414369555700318 1104 24-25 -0.8931182218342926 1104 26-27 -0.8580462712900996 1104 28-29 -0.8801669766865032 1104 30-31 -0.9791209801381058 1104 32-33 -1.0052811409415625 1104 34-35 -1.0125853833139686 1104 36-37 -1.0200489010144764 1104 38-39 -1.025792484743036 1104 40-41 -1.0312180675114249 1104 42-43 -1.0057491719129246 1104 44-45 -1.0153302682964807 1104 46-47 -0.9678195566187142 1104 48-49 -0.9360964650696957 1104 50-51 -1.0213130826195425 1104 52-53 -0.8749633750284076 1104 54-55 -0.7628377024915522 1104 56-57 -0.7141896650049446 1104 58-59 -0.7556474207068433 1104 60-61 -0.7035597402823583 1104 62-63 -0.8505492404697108 1104 64-65 -0.7261780341875905 1104 66-67 -0.8123301865608283 1104 68-69 -0.8713701806552265 1104 70-71 -0.807284394780666 1104 72-73 -0.7623169694628231 1104 74-75 -0.7286777416240398 1104 76-77 -0.7240961789978719 1104 78-79 -0.7739291710992013 1104 80-81 -0.7997316889052151 1104 82-83 -0.8165887609841 1104 84-85 -0.857617172912672 1104 86-87 -0.8667133772752358 1104 88-89 -0.9612558201955856 1104 90-91 -1.0676892483461593 1104 92-93 -1.1226914641550607 1104 94-95 -0.9897367962911368 1104 96-97 -1.1371997763413795 1104 98-99 -1.178667991962918 1104 100 -1.1833551777782247 1105 1 -0.6242566297162107 1105 2 -0.6064562746534499 1105 3 -0.6056236651058384 1105 4 -0.41454763787134397 1105 5 -0.42994557534782274 1105 6 -0.42440270075549336 1105 7 -0.3656835945753585 1105 8 -0.42671990830746154 1105 9 -0.6466188196190856 1105 10-11 -0.6908918886724322 1105 12-13 -0.6971562364162267 1105 14-15 -0.9464483335881937 1105 16-17 -0.9234268160960468 1105 18-19 -0.8739361810683945 1105 20-21 -0.8803256457505952 1105 22-23 -0.904592521907702 1105 24-25 -0.9523292541845692 1105 26-27 -0.8507615878414683 1105 28-29 -0.8791559774398507 1105 30-31 -0.9545484164694145 1105 32-33 -1.0032636657986842 1105 34-35 -1.0162881310363687 1105 36-37 -0.9910406445814814 1105 38-39 -0.9931439859882829 1105 40-41 -1.033371932825922 1105 42-43 -0.9528644812431608 1105 44-45 -1.0633171035211717 1105 46-47 -1.0891729045268264 1105 48-49 -1.170661728497052 1105 50-51 -1.1168589414820858 1105 52-53 -0.9088036244901687 1105 54-55 -0.8532873275808512 1105 56-57 -0.8019141844446054 1105 58-59 -0.7515828962286193 1105 60-61 -0.7543634666306076 1105 62-63 -0.7868423020558382 1105 64-65 -0.7761435816732387 1105 66-67 -0.75531802990443 1105 68-69 -0.8475512112245482 1105 70-71 -0.779944281019425 1105 72-73 -0.7830484986757611 1105 74-75 -0.7991196242790011 1105 76-77 -0.7572373882618564 1105 78-79 -0.8674046336472792 1105 80-81 -0.833078883028346 1105 82-83 -0.9729003865626531 1105 84-85 -0.9689419993722019 1105 86-87 -1.029492278340438 1105 88-89 -1.0943322218155522 1105 90-91 -1.219664857142476 1105 92-93 -1.183763317678018 1105 94-95 -1.3116806746969907 1105 96-97 -1.1541911032733267 1105 98-99 -1.1553918144414936 1105 100 -1.2175294148025984 1106 1 -0.5820315940432188 1106 2 -0.5540697835202657 1106 3 -0.6095911236208984 1106 4 -0.4818665184252424 1106 5 -0.47483188987708047 1106 6 -0.4322131607240536 1106 7 -0.49760076588297153 1106 8 -0.5100899729270125 1106 9 -0.6557381570151293 1106 10-11 -0.5414365931473668 1106 12-13 -0.6462291082439364 1106 14-15 -0.932845954116253 1106 16-17 -0.9011410486542957 1106 18-19 -0.8711136355957549 1106 20-21 -0.809350049666115 1106 22-23 -0.8885313860731543 1106 24-25 -0.812792894707492 1106 26-27 -0.9007626319778623 1106 28-29 -0.9875569789714902 1106 30-31 -0.9668604232086366 1106 32-33 -0.9318327563039333 1106 34-35 -0.9595929136506669 1106 36-37 -0.984233444816411 1106 38-39 -0.9991918837720277 1106 40-41 -1.1513757718506668 1106 42-43 -1.064715459299208 1106 44-45 -1.1016381082181894 1106 46-47 -0.9929889083101031 1106 48-49 -0.9872185843215959 1106 50-51 -1.0332690087154717 1106 52-53 -1.0667386963921928 1106 54-55 -0.8569433536816575 1106 56-57 -0.8676681992989188 1106 58-59 -0.8324687855095974 1106 60-61 -0.7531687148098385 1106 62-63 -0.839103846532403 1106 64-65 -1.0040316354639458 1106 66-67 -0.9070448466542871 1106 68-69 -0.9501884799395981 1106 70-71 -0.8797073170123539 1106 72-73 -0.8513202311258468 1106 74-75 -0.8776531891674821 1106 76-77 -0.8390131815032653 1106 78-79 -0.9121854654213202 1106 80-81 -0.8397199202264787 1106 82-83 -0.9889179584289636 1106 84-85 -0.9992096450025016 1106 86-87 -1.0450730115160276 1106 88-89 -1.0084282030294922 1106 90-91 -1.0571942965431447 1106 92-93 -1.0384303026147776 1106 94-95 -1.1474310755404495 1106 96-97 -1.1049145698940812 1106 98-99 -1.0944820529052635 1106 100 -1.215330293695665 1107 1 -0.6122616634673825 1107 2 -0.5963684389164818 1107 3 -0.5683031248855741 1107 4 -0.4786555831221406 1107 5 -0.5582459808598159 1107 6 -0.41426747970290023 1107 7 -0.464653220844923 1107 8 -0.48138618121625143 1107 9 -0.694885466551753 1107 10-11 -0.6270470722218633 1107 12-13 -0.655422380448023 1107 14-15 -0.6895934736441092 1107 16-17 -0.7977511480307697 1107 18-19 -0.7872409521353703 1107 20-21 -0.8488282529803755 1107 22-23 -0.8977860698772844 1107 24-25 -0.83220782486719 1107 26-27 -0.8758536043741572 1107 28-29 -0.8985250808901242 1107 30-31 -0.954862556609207 1107 32-33 -0.9891802085597377 1107 34-35 -1.052354027451237 1107 36-37 -1.020804274257138 1107 38-39 -0.909388573258326 1107 40-41 -0.9628449668169807 1107 42-43 -0.9776842890955066 1107 44-45 -0.9603318879174907 1107 46-47 -0.874535535079751 1107 48-49 -0.8670465727444991 1107 50-51 -0.9018812296731298 1107 52-53 -0.8571315976642992 1107 54-55 -0.7314837393189322 1107 56-57 -0.6515713176982132 1107 58-59 -0.7268655556632595 1107 60-61 -0.7719526863329804 1107 62-63 -0.7199251565053615 1107 64-65 -0.68695916140382 1107 66-67 -0.6637771979601581 1107 68-69 -0.6361947117149569 1107 70-71 -0.7114468707612929 1107 72-73 -0.6606538586307593 1107 74-75 -0.6223185796679402 1107 76-77 -0.6922111400969726 1107 78-79 -0.6469374871532416 1107 80-81 -0.7635253611858985 1107 82-83 -0.783592632078264 1107 84-85 -0.7204075799574241 1107 86-87 -0.8299346335012778 1107 88-89 -0.833212782266461 1107 90-91 -0.8245649394344454 1107 92-93 -0.9261929947968888 1107 94-95 -1.0272090777772718 1107 96-97 -0.9296737374344843 1107 98-99 -0.8368315316946209 1107 100 -0.9133662551861121 1108 1 -0.5962988904460182 1108 2 -0.5429278719869828 1108 3 -0.5761737950601109 1108 4 -0.4336223053966606 1108 5 -0.42680447446040404 1108 6 -0.49707914304791245 1108 7 -0.4499911110583952 1108 8 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1.3231113094186178 2310 96-97 1.318464753385964 2310 98-99 1.3675423694203275 2310 100 1.5177270886439373 2311 1 0.6874372757665697 2311 2 0.6607640729812907 2311 3 0.6551958599559065 2311 4 0.5737897097833695 2311 5 0.5861195183359911 2311 6 0.6066642065038863 2311 7 0.667310157808167 2311 8 0.6070917257013022 2311 9 0.8310048178155043 2311 10-11 0.8249889497482599 2311 12-13 0.8742913724565042 2311 14-15 1.1140105824579436 2311 16-17 1.0676481516430982 2311 18-19 1.023482833627959 2311 20-21 1.0767347837917711 2311 22-23 1.1131499632473805 2311 24-25 1.1416915184513954 2311 26-27 1.183060625223213 2311 28-29 1.1888621070960426 2311 30-31 1.160545519881218 2311 32-33 1.20494368881279 2311 34-35 1.1940594062484493 2311 36-37 1.2401243783626867 2311 38-39 1.2977917882475722 2311 40-41 1.367168227131991 2311 42-43 1.3444417463095277 2311 44-45 1.3625826965824288 2311 46-47 1.3653453220290501 2311 48-49 1.367057543279266 2311 50-51 1.389109791005204 2311 52-53 1.2998970605567237 2311 54-55 1.193325376889149 2311 56-57 1.1265284823658988 2311 58-59 1.0562030396263893 2311 60-61 1.0649892058752641 2311 62-63 1.1416807073017523 2311 64-65 1.1282818842884481 2311 66-67 1.1180453367238243 2311 68-69 1.120187613755256 2311 70-71 1.0858155045101725 2311 72-73 1.0922716857193748 2311 74-75 1.1592169291775747 2311 76-77 1.141523831000974 2311 78-79 1.1499092500013681 2311 80-81 1.103611173685433 2311 82-83 1.1745311255035062 2311 84-85 1.1889427935918704 2311 86-87 1.1833139950413418 2311 88-89 1.2638821552817028 2311 90-91 1.2892477910666997 2311 92-93 1.3145624880405258 2311 94-95 1.3576474029203531 2311 96-97 1.3747083116457937 2311 98-99 1.419298675730282 2311 100 1.4966746942484157 2312 1 0.7026728569457887 2312 2 0.6439761604652254 2312 3 0.6891382700732329 2312 4 0.37337512482716306 2312 5 0.5166072626547944 2312 6 0.566347704176998 2312 7 0.4592203950195568 2312 8 0.5704396106591645 2312 9 0.7704995605021523 2312 10-11 0.7448827009271355 2312 12-13 0.9180795076915658 2312 14-15 1.121400095068985 2312 16-17 1.1112222594229593 2312 18-19 1.0550076617114499 2312 20-21 1.0460817154281372 2312 22-23 1.1209100536578376 2312 24-25 1.1242388511154573 2312 26-27 1.1429791795022268 2312 28-29 1.1466546116699519 2312 30-31 1.191316289864993 2312 32-33 1.2242370615902018 2312 34-35 1.2419090433530968 2312 36-37 1.2138547581522303 2312 38-39 1.2551086591366527 2312 40-41 1.3023444449958461 2312 42-43 1.353165249130285 2312 44-45 1.4041437574560902 2312 46-47 1.4377800134209977 2312 48-49 1.440758502799099 2312 50-51 1.4392676919395768 2312 52-53 1.2617775909473394 2312 54-55 1.179940386649605 2312 56-57 1.092348922998248 2312 58-59 1.134420734627092 2312 60-61 1.1311154601716709 2312 62-63 1.122020377128159 2312 64-65 1.1489396869070418 2312 66-67 1.114509627813689 2312 68-69 1.062335662673739 2312 70-71 1.0688256710646442 2312 72-73 1.1088082241383503 2312 74-75 1.132666933129208 2312 76-77 1.0944530170947786 2312 78-79 1.0899012075708576 2312 80-81 1.1116321770568227 2312 82-83 1.183531807423151 2312 84-85 1.2086631171850541 2312 86-87 1.1661784724613007 2312 88-89 1.1379296875301215 2312 90-91 1.1605309657074017 2312 92-93 1.3184230061772304 2312 94-95 1.3412504426006429 2312 96-97 1.31631638127298 2312 98-99 1.0073771680238792 2312 100 1.2130708175103564 2313 1 0.6860692085039233 2313 2 0.6506788758819759 2313 3 0.617801243804923 2313 4 0.5588502155977508 2313 5 0.439743520663086 2313 6 0.5215310993313196 2313 7 0.6087376286449597 2313 8 0.6342782778286349 2313 9 0.7905278172093517 2313 10-11 0.8418838918553178 2313 12-13 0.8824305912676991 2313 14-15 1.0847454646336345 2313 16-17 1.0748067679759004 2313 18-19 1.0459716198261688 2313 20-21 1.0401482848248307 2313 22-23 1.136989370006333 2313 24-25 1.115799283329956 2313 26-27 1.1061750795009146 2313 28-29 1.1869300761353827 2313 30-31 1.1372289999340879 2313 32-33 1.106813277889401 2313 34-35 1.1877690110504275 2313 36-37 1.1639847763511781 2313 38-39 1.2635489043697135 2313 40-41 1.2734191709596772 2313 42-43 1.2629475746367973 2313 44-45 1.28072650898104 2313 46-47 1.2881172640599416 2313 48-49 1.235400892767089 2313 50-51 1.2781073765121107 2313 52-53 1.1933625068556495 2313 54-55 1.080942967158208 2313 56-57 1.0097289214157286 2313 58-59 1.0595553274446488 2313 60-61 1.0227081585975384 2313 62-63 1.0194470568556895 2313 64-65 0.9887312345687533 2313 66-67 1.0333618552661932 2313 68-69 1.0443526078296443 2313 70-71 1.0116497252714822 2313 72-73 1.0575672732364936 2313 74-75 1.0800819755393931 2313 76-77 0.839761347866947 2313 78-79 0.9886510901876697 2313 80-81 1.0969305919674888 2313 82-83 1.1453099944394722 2313 84-85 1.1567451498272874 2313 86-87 1.156331685971974 2313 88-89 1.1971923442459165 2313 90-91 1.1989565056478888 2313 92-93 1.2723881904966667 2313 94-95 1.3523259957895384 2313 96-97 1.348594023030234 2313 98-99 1.421796351092084 2313 100 1.5379570777603853 2314 1 0.5655661521492839 2314 2 0.5690803942542111 2314 3 0.5680573634731978 2314 4 0.4454301968894825 2314 5 0.43683168224126234 2314 6 0.4384727202100791 2314 7 0.5048661251046198 2314 8 0.5015392141754873 2314 9 0.6480808066110626 2314 10-11 0.6367600670291722 2314 12-13 0.6877646436886309 2314 14-15 0.8532995675698487 2314 16-17 0.8139835798239972 2314 18-19 0.7919867268193812 2314 20-21 0.8383630142970517 2314 22-23 0.8867909666846288 2314 24-25 0.9076047111304248 2314 26-27 0.8864802178602886 2314 28-29 0.9392870943763043 2314 30-31 0.9940412235065494 2314 32-33 0.9686061069136827 2314 34-35 1.0095996431339032 2314 36-37 1.0056807581127103 2314 38-39 1.0557316259986322 2314 40-41 1.1044804942984783 2314 42-43 1.1537818002993134 2314 44-45 1.2394164795215588 2314 46-47 1.2455328019960987 2314 48-49 1.2355795549132012 2314 50-51 1.2682490120251728 2314 52-53 1.2179479325532796 2314 54-55 1.063688885235976 2314 56-57 1.0522005252520543 2314 58-59 1.0131314441052908 2314 60-61 0.9422528743253267 2314 62-63 1.0168442624811647 2314 64-65 1.0110050317055652 2314 66-67 1.047888235558375 2314 68-69 1.0002632612280635 2314 70-71 1.0414110828835987 2314 72-73 1.0607337698185617 2314 74-75 1.1088563404768976 2314 76-77 1.0606983771586016 2314 78-79 1.07490038245 2314 80-81 1.0832890910910358 2314 82-83 1.0771011018649688 2314 84-85 1.1169066822819218 2314 86-87 1.1764107030770568 2314 88-89 1.173346416375086 2314 90-91 1.2775088367459944 2314 92-93 1.2338568592017864 2314 94-95 1.2559964386115716 2314 96-97 1.3599648440126622 2314 98-99 1.4179532219610707 2314 100 1.4741880446830464 2315 1 0.4685523292841225 2315 2 0.49901397292774163 2315 3 0.5663338798420874 2315 4 0.2640507642158738 2315 5 0.3544140894885359 2315 6 0.3832822702548455 2315 7 0.44174104659250446 2315 8 0.43849544980631094 2315 9 0.626451052895149 2315 10-11 0.5102691065632143 2315 12-13 0.584276935488866 2315 14-15 0.7957520318334659 2315 16-17 0.7974026831843162 2315 18-19 0.6416972181589173 2315 20-21 0.7734488389368153 2315 22-23 0.6244563018976095 2315 24-25 0.7814629450080943 2315 26-27 0.8520322785304444 2315 28-29 0.8890038636900499 2315 30-31 0.9382343160216635 2315 32-33 0.9119170242449215 2315 34-35 0.954328951323312 2315 36-37 1.0284623104292976 2315 38-39 1.063134363974143 2315 40-41 1.1115354217204825 2315 42-43 1.1219731261158046 2315 44-45 1.1225768740347277 2315 46-47 1.1622324469970309 2315 48-49 1.1346880226723641 2315 50-51 1.0508213137108342 2315 52-53 1.1166126110350376 2315 54-55 1.008545112737778 2315 56-57 0.9579719903377395 2315 58-59 0.9337875187671827 2315 60-61 0.9798964283224691 2315 62-63 0.9688589021419247 2315 64-65 1.0211980117203439 2315 66-67 1.0266705278132875 2315 68-69 1.0112475774946788 2315 70-71 0.9900813115019034 2315 72-73 0.7302360720319712 2315 74-75 1.0028324624895575 2315 76-77 1.0546356032872168 2315 78-79 1.0680956095362468 2315 80-81 0.7604906757044425 2315 82-83 0.7755725888696574 2315 84-85 1.0733854887575518 2315 86-87 1.1655625074285432 2315 88-89 1.1493111202622721 2315 90-91 1.2298424010904085 2315 92-93 1.3103414847844235 2315 94-95 1.3380181655540788 2315 96-97 1.2177988842071557 2315 98-99 0.5844762959423839 2315 100 1.1171451190943529 2316 1 0.25586632950134813 2316 2 0.22598037468911158 2316 3 0.2671576819234076 2316 4 0.2390462079920752 2316 5 0.09246380479358152 2316 6 0.13347933843988358 2316 7 0.12807366381631624 2316 8 0.16958877783785908 2316 9 0.21310785234582852 2316 10-11 0.22526285671278856 2316 12-13 0.019730615721115896 2316 14-15 0.06933219283845204 2316 16-17 0.23573644912879388 2316 18-19 0.24239399824333674 2316 20-21 0.3132540350569357 2316 22-23 0.2675517359170385 2316 24-25 0.35528150236957146 2316 26-27 0.3845788118424238 2316 28-29 0.3529686436198318 2316 30-31 0.2900760429317373 2316 32-33 0.4354867841733494 2316 34-35 0.4503872194428098 2316 36-37 0.38862600312442197 2316 38-39 0.5466675268402739 2316 40-41 0.6066912813924787 2316 42-43 0.4886464200170906 2316 44-45 0.4621464351461171 2316 46-47 0.3148970555391628 2316 48-49 0.25412073138883784 2316 50-51 0.28869780046648685 2316 52-53 0.34128451689885253 2316 54-55 0.04085490650192014 2316 56-57 -0.028635288943135606 2316 58-59 0.039035552227353776 2316 60-61 0.09819716854775606 2316 62-63 0.2681681970225682 2316 64-65 0.19919025417404157 2316 66-67 0.10644347139158583 2316 68-69 0.12269502076278371 2316 70-71 -0.09431210323595707 2316 72-73 -0.13111371157040708 2316 74-75 0.10837319881214569 2316 76-77 0.21654281095713657 2316 78-79 0.29549719970438204 2316 80-81 0.4433513583464297 2316 82-83 0.47982451214836885 2316 84-85 0.6229702740995862 2316 86-87 0.6196873166654342 2316 88-89 0.6220676632757893 2316 90-91 0.5057061966917651 2316 92-93 0.5104287433735948 2316 94-95 0.6672722518885337 2316 96-97 0.6387646931992101 2316 98-99 0.5172875504594856 2316 100 0.4464277393556486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 32.0 1 497.0 2 193.0 3 157.0 4 142.0 5 101.0 6 112.0 7 513.0 8 1699.0 9 3182.0 10 5122.0 11 7632.0 12 10983.0 13 14136.0 14 17035.0 15 20194.0 16 25196.0 17 31836.0 18 39670.0 19 48829.0 20 62317.0 21 77790.0 22 85515.0 23 84960.0 24 81302.0 25 82103.0 26 90020.0 27 102866.0 28 121681.0 29 146287.0 30 177274.0 31 221800.0 32 281053.0 33 372573.0 34 547025.0 35 739954.0 36 1094104.0 37 1476688.0 38 1198855.0 39 368939.0 40 318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.982781686599566 21.458292301180887 13.119096608194978 22.43982940402457 2 15.57656094354664 19.905972443063906 41.81657654403777 22.70089006935168 3 19.02056806539076 27.462419333957904 28.528070454004894 24.988942146646444 4 12.343029825920237 16.40165776787768 39.826157164363984 31.429155241838103 5 12.697176389335779 38.939821913895464 34.361648680114975 14.00135301665378 6 28.9824423473564 38.44053872817504 18.359200217580124 14.217818706888435 7 27.1319941966758 32.51134415260349 23.12001878393408 17.236642866786628 8 26.727536564363962 35.238074287306546 19.708694996323135 18.325694152006353 9 27.22956981616671 15.253656528540747 21.91239571947734 35.60437793581521 10-11 26.146390252677993 26.336894474801465 26.750648696335745 20.766066576184798 12-13 26.064021449855414 24.04634139762913 27.680367773158192 22.209269379357263 14-15 23.57322606893504 25.078509612011324 25.74374357916387 25.604520739889768 16-17 21.55846916695078 27.795419423444244 27.207517044967005 23.438594364637968 18-19 20.938951231266227 27.822740023461538 29.87286614413696 21.365442601135275 20-21 22.543878796962854 27.009199075936145 29.20721827523135 21.239703851869645 22-23 23.06016591059862 26.634951191310364 28.829504555881343 21.475378342209677 24-25 22.279398283771233 26.54608551217612 29.807776329830528 21.36673987422212 26-27 22.017451934332744 26.87016544445267 28.79226197523946 22.32012064597513 28-29 21.17403293943684 27.56136349832025 29.25735204795456 22.00725151428835 30-31 22.649944010483054 26.92366320733112 29.15546838148375 21.270924400702075 32-33 22.881908744524413 27.130861408688627 28.213012439215774 21.77421740757119 34-35 22.647619880444207 27.09090418780397 29.22278917085922 21.038686760892606 36-37 22.071809930959567 26.81994250634202 28.903271066973456 22.204976495724956 38-39 22.51755317004308 27.007049020064734 29.23612096541519 21.239276844477 40-41 22.17386196019753 25.805312908786306 29.889580856274456 22.131244274741707 42-43 22.030443049840226 26.250803840840558 28.956860716114836 22.761892393204377 44-45 21.938780653894767 27.24344828010602 29.009311894455507 21.808459171543713 46-47 22.005017585116942 26.829382465163658 28.271584650798598 22.8940152989208 48-49 22.807008126061845 26.846275188382602 28.192965949726933 22.153750735828623 50-51 22.46793402980856 26.5824927516955 28.251901990577878 22.69767122791806 52-53 21.85904715218119 27.34108870295659 27.929386228326976 22.870477916535243 54-55 21.681861197333905 27.092012711453872 28.124209527410642 23.10191656380158 56-57 22.798064496109497 26.66990367913067 27.362009850870045 23.17002197388979 58-59 21.797731741516944 27.22352089013505 28.389919334195156 22.58882803415285 60-61 22.1994322164165 27.855006777219433 27.6093473002265 22.336213706137567 62-63 22.54986861590601 26.95604026459049 27.29663611685382 23.197455002649676 64-65 21.62039104960462 27.026509924956056 27.725036275692116 23.628062749747208 66-67 22.20852279779152 26.660424791403624 27.5542353194393 23.576817091365555 68-69 21.539883540277117 26.84079892511609 27.95996994891892 23.659347585687872 70-71 21.73027794255329 26.353154643429903 28.133424104799797 23.783143309217007 72-73 22.36256091534662 26.710349998258533 28.024954833147795 22.90213425324705 74-75 22.548226675619485 26.953937540346267 27.642254177912783 22.85558160612147 76-77 22.133815452114646 26.674028394600484 27.447225822869783 23.744930330415084 78-79 21.931611754136487 26.723598400816634 27.556007874699134 23.788781970347745 80-81 21.5644974594064 27.22164155833547 28.044726072247794 23.16913491001033 82-83 22.66910867997757 26.224811665112373 29.10605923352793 22.00002042138213 84-85 22.747092400170033 26.826655968134784 27.566098176264642 22.860153455430545 86-87 21.24268706160657 27.6340453393672 28.132124936285447 22.991142662740778 88-89 22.30107569108325 26.147036204351853 28.09863501444836 23.453253090116537 90-91 22.30231342996859 26.071032089841843 28.26893750200041 23.35771697818916 92-93 22.66406070619862 26.344900233716977 27.979007884904533 23.01203117517987 94-95 21.28809151014797 26.44931878523458 28.60078305874394 23.661806645873508 96-97 21.605238679317964 26.3078387161955 28.17416698745241 23.912755617034126 98-99 20.899205779935308 26.79546532508798 28.03310648874664 24.272222406230075 100 21.741534737076652 26.422072660781637 27.023082207051374 24.813310395090333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3112.0 1 2083.5 2 1529.0 3 1959.0 4 1533.5 5 1292.0 6 1896.0 7 3369.0 8 5290.0 9 6101.5 10 5251.5 11 4513.0 12 4902.0 13 5811.0 14 6913.5 15 8253.5 16 9550.0 17 10629.5 18 11525.5 19 12092.5 20 12529.5 21 13066.0 22 14049.5 23 15340.0 24 16353.5 25 17838.0 26 20987.0 27 24596.5 28 29873.0 29 42797.0 30 63191.5 31 83365.5 32 113055.0 33 146271.5 34 171355.5 35 196917.5 36 221526.5 37 258851.0 38 270700.0 39 250405.5 40 227074.0 41 200606.0 42 190339.5 43 198691.0 44 219202.5 45 261226.5 46 300081.5 47 324881.0 48 343310.0 49 343743.5 50 334437.5 51 347337.5 52 350327.0 53 327149.5 54 304106.5 55 257931.5 56 222120.0 57 183027.0 58 141518.0 59 130657.5 60 105140.0 61 69613.5 62 50152.0 63 42063.0 64 30848.5 65 14460.0 66 7658.5 67 4920.0 68 3608.0 69 2902.5 70 2375.5 71 1957.0 72 1656.0 73 1514.5 74 1240.5 75 983.5 76 740.0 77 485.5 78 354.5 79 265.0 80 184.0 81 139.5 82 125.5 83 96.0 84 62.5 85 46.0 86 39.5 87 38.0 88 27.0 89 14.0 90 16.0 91 15.5 92 8.5 93 10.0 94 9.5 95 8.0 96 8.5 97 5.5 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05754719635739466 2 0.132920019605572 3 0.01724976229225521 4 0.016438316721602838 5 0.019095146573900115 6 0.0518016382039045 7 0.05722000056277677 8 0.0489615787066212 9 0.040742420345819784 10-11 0.04003567742944514 12-13 0.041239757953638975 14-15 0.019540132854580446 16-17 0.018408035405202545 18-19 0.017989224788091646 20-21 0.02097325043500681 22-23 0.025292234923962972 24-25 0.024886512138636782 26-27 0.019298007966563207 28-29 0.019016619583191823 30-31 0.017125427890300412 32-33 0.023283252745009118 34-35 0.022785915137189923 36-37 0.022975688698068302 38-39 0.021058321341607462 40-41 0.024048890904414984 42-43 0.03084801951657476 44-45 0.03970848163482724 46-47 0.04639636367681693 48-49 0.039747745130181394 50-51 0.02845949021586415 52-53 0.041069616140437665 54-55 0.037287232754654856 56-57 0.03905409004559146 58-59 0.04413216877806113 60-61 0.024330279287786367 62-63 0.02634580538263258 64-65 0.025665238129827368 66-67 0.03257561331215722 68-69 0.03956451548519537 70-71 0.041743639477350526 72-73 0.0451137561619148 74-75 0.027772379047166583 76-77 0.021293902313732342 78-79 0.020102909621323217 80-81 0.02511554919486931 82-83 0.021372429304440634 84-85 0.013185990523101003 86-87 0.014959391729929973 88-89 0.01845384281644905 90-91 0.02115648007999283 92-93 0.02003747046239964 94-95 0.023486114137672213 96-97 0.024729458157220198 98-99 0.027772379047166583 100 0.028845581253513268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7640685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.71191418379206 #Duplication Level Percentage of deduplicated Percentage of total 1 80.28273552250394 51.95249491572349 2 12.519777157858655 16.203574900790986 3 3.657080543483519 7.099700468793634 4 1.4549847251666803 3.766193866948579 5 0.699689177033286 2.263911298975305 6 0.3836486123130504 1.4895981646039815 7 0.21622941442426186 0.979483351716411 8 0.13903697757387104 0.719787916890733 9 0.10118949632294749 0.5893349402116548 >10 0.47715456105742277 5.501731117553235 >50 0.03631460817961219 1.6331537081870038 >100 0.027694126412211518 3.4692989107084062 >500 0.0030811831075147136 1.3434728738577746 >1k 0.0012533384721111608 1.4704505404555706 >5k 5.222243630663468E-5 0.27140072362266965 >10k+ 7.833365445995201E-5 1.2464123009604176 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32012 0.418967671092317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25369 0.332025204546451 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16384 0.214431035960781 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9892 0.1294648320144071 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.543915892357819E-6 0.0 12-13 0.0 0.0 0.0 2.6175663569431275E-5 0.0 14-15 0.0 0.0 0.0 2.6175663569431275E-5 0.0 16-17 0.0 0.0 0.0 3.9263495354146915E-5 0.0 18-19 0.0 0.0 0.0 5.889524303122037E-5 0.0 20-21 0.0 0.0 0.0 1.3742223373951418E-4 0.0 22-23 0.0 0.0 0.0 4.2535453300325825E-4 0.0 24-25 0.0 0.0 0.0 9.357799726071681E-4 0.0 26-27 0.0 0.0 0.0 0.002080965253769786 0.0 28-29 0.0 0.0 0.0 0.005490345433688209 0.0 30-31 0.0 0.0 0.0 0.019010075667299463 0.0 32-33 0.0 0.0 0.0 0.050191834894384474 0.0 34-35 0.0 0.0 0.0 0.09406879095264364 0.0 36-37 0.0 0.0 0.0 0.16594061919840958 0.0 38-39 0.0 0.0 0.0 0.276866537489767 0.0 40-41 0.0 0.0 0.0 0.3989105688822403 0.0 42-43 0.0 0.0 0.0 0.5127223540821275 0.0 44-45 0.0 0.0 0.0 0.6427826824427391 0.0 46-47 0.0 0.0 0.0 0.7956420137723255 0.0 48-49 0.0 0.0 0.0 0.9670140831613919 0.0 50-51 0.0 0.0 0.0 1.1490265597914324 0.0 52-53 0.0 0.0 0.0 1.3255748666513538 0.0 54-55 0.0 0.0 0.0 1.5126130706867251 6.543915892357819E-6 56-57 0.0 0.0 0.0 1.72572223563725 1.3087831784715637E-5 58-59 0.0 0.0 0.0 1.979037219830421 1.9631747677073454E-5 60-61 0.0 0.0 0.0 2.2348977873057194 2.6175663569431275E-5 62-63 0.0 0.0 0.0 2.5004368063858147 2.6175663569431275E-5 64-65 0.0 0.0 0.0 2.757992248077234 2.6175663569431275E-5 66-67 0.0 0.0 0.0 3.045832932518485 2.6175663569431275E-5 68-69 0.0 0.0 0.0 3.3652022037291163 2.6175663569431275E-5 70-71 0.0 0.0 0.0 3.7098898855272795 2.6175663569431275E-5 72-73 0.0 0.0 0.0 4.053046291006631 2.6175663569431275E-5 74-75 6.543915892357819E-6 0.0 0.0 4.379620413614748 2.6175663569431275E-5 76-77 1.3087831784715637E-5 0.0 0.0 4.746930412652793 2.6175663569431275E-5 78-79 1.3087831784715637E-5 0.0 0.0 5.137176836893551 2.6175663569431275E-5 80-81 1.3087831784715637E-5 6.543915892357819E-6 0.0 5.54968566300011 2.6175663569431275E-5 82-83 1.3087831784715637E-5 1.3087831784715637E-5 0.0 5.971225093038124 2.6175663569431275E-5 84-85 1.3087831784715637E-5 1.3087831784715637E-5 0.0 6.374886544858216 2.6175663569431275E-5 86-87 1.3087831784715637E-5 1.3087831784715637E-5 0.0 6.797394474448299 2.6175663569431275E-5 88 1.3087831784715637E-5 1.3087831784715637E-5 0.0 7.139058867104193 2.6175663569431275E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15945 0.0 49.057373 1 GTATCAA 28820 0.0 40.777664 1 GTACTAG 7860 0.0 39.63996 1 ACCTGGG 3435 0.0 39.41356 3 GCATCGT 7685 0.0 37.7397 8 TACCTGG 3785 0.0 36.682556 2 GGGCACC 8235 0.0 35.783623 94 TAGGCAT 9860 0.0 34.80328 5 CAACGCA 33715 0.0 33.2676 5 ATCAACG 33890 0.0 33.110123 3 GTGGTAT 5190 0.0 33.008957 1 CTAGGCA 10915 0.0 31.999 4 TATCAAC 36915 0.0 31.6193 2 CTGTGCG 10275 0.0 31.062916 9 AACGCAG 38810 0.0 29.845171 6 ACTGTGC 11080 0.0 29.697195 8 TAGTACT 11120 0.0 29.464518 4 CCTGGGG 3875 0.0 29.357227 4 GTACATG 41980 0.0 29.070858 1 TCTAACG 3950 0.0 28.954273 2 >>END_MODULE