FastQCFastQC Report
Mon 6 Jun 2016
ERR1211228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7640685
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294240.3850963624334729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244310.3197488183323877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159740.20906502492904758No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC102910.13468687689650863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165600.048.4374771
GTATCAA297900.040.688831
ACCTGGG35650.039.0261463
GTACTAG80750.038.4294551
TACCTGG37850.037.871452
GGGCACC82950.034.2313294
CAACGCA343100.033.4839485
ATCAACG346300.033.1991843
TAGGCAT97650.033.0569655
GTACACG5150.032.866851
GTGGTAT48200.031.9955521
CCTGGGG37100.031.9264134
TATCAAC386450.031.108942
AACGCAG392900.030.0769966
CTAGGCA113350.029.8561944
CTGTGCG106950.029.4353839
TCTAACG41100.029.3879492
TAACGCC41700.029.1935674
GCATCGT98650.029.1494248
CTAACGC41300.029.0210843
Produced by FastQC (version 0.11.5)