##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211228_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7640685 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1409963897216 33.0 31.0 34.0 30.0 34.0 2 32.28948346908687 34.0 31.0 34.0 30.0 34.0 3 32.360648161781306 34.0 31.0 34.0 30.0 34.0 4 35.978975314386076 37.0 35.0 37.0 35.0 37.0 5 35.87286427853 37.0 35.0 37.0 35.0 37.0 6 35.89856079657779 37.0 35.0 37.0 35.0 37.0 7 35.83888538789389 37.0 35.0 37.0 35.0 37.0 8 35.8288975399457 37.0 35.0 37.0 35.0 37.0 9 37.413691311708305 39.0 37.0 39.0 34.0 39.0 10-11 37.39401879543523 39.0 37.0 39.0 34.0 39.0 12-13 37.275212104673855 39.0 37.0 39.0 33.5 39.0 14-15 38.45668379209456 40.0 38.0 41.0 34.0 41.0 16-17 38.41716155030602 40.0 38.0 41.0 34.0 41.0 18-19 38.45463554642025 40.0 38.0 41.0 34.0 41.0 20-21 38.419346694700806 40.0 38.0 41.0 34.0 41.0 22-23 38.28513987947416 40.0 38.0 41.0 34.0 41.0 24-25 38.21151683389644 40.0 37.5 41.0 34.0 41.0 26-27 38.054754972885284 40.0 37.0 41.0 33.0 41.0 28-29 38.04154247950282 40.0 37.0 41.0 33.0 41.0 30-31 38.038754116941085 40.0 37.0 41.0 33.0 41.0 32-33 37.892851491718346 40.0 37.0 41.0 33.0 41.0 34-35 37.75526631447312 40.0 36.5 41.0 33.0 41.0 36-37 37.62312495541957 40.0 36.0 41.0 33.0 41.0 38-39 37.519412657372996 40.0 36.0 41.0 32.5 41.0 40-41 37.405055175026845 40.0 36.0 41.0 32.0 41.0 42-43 37.24585518183252 39.0 36.0 41.0 32.0 41.0 44-45 37.05832160336409 39.0 35.0 41.0 31.0 41.0 46-47 36.771905267655974 39.0 35.0 41.0 31.0 41.0 48-49 36.47752407015864 39.0 35.0 41.0 30.0 41.0 50-51 36.24810969173575 39.0 35.0 40.0 30.0 41.0 52-53 36.02869925929416 38.0 35.0 40.0 29.0 41.0 54-55 35.771506154225705 38.0 35.0 40.0 29.0 41.0 56-57 35.47954993564059 38.0 34.0 40.0 28.0 41.0 58-59 35.17197528755602 37.0 34.0 40.0 28.0 41.0 60-61 34.90052966455232 36.5 34.0 40.0 27.0 41.0 62-63 34.57390417220445 36.0 34.0 39.0 26.5 41.0 64-65 34.19976762554666 35.5 33.0 39.0 26.0 40.5 66-67 33.90512820512821 35.0 33.0 38.5 26.0 40.0 68-69 33.60046049536135 35.0 33.0 38.0 26.0 40.0 70-71 33.334101654498255 35.0 32.5 37.5 25.5 40.0 72-73 33.03535887161949 35.0 32.0 37.0 25.0 39.5 74-75 32.721717751746084 35.0 32.0 36.0 25.0 39.0 76-77 31.761306152524284 34.0 30.5 35.0 23.5 38.0 78-79 32.28607421978526 34.5 31.5 36.0 25.0 38.0 80-81 32.254277070707666 35.0 32.0 35.0 25.0 37.0 82-83 32.028157292179955 34.5 32.0 35.0 24.5 37.0 84-85 31.80060563941584 34.0 31.0 35.0 24.0 36.5 86-87 31.58510513128077 34.0 31.0 35.0 24.0 36.0 88-89 31.394934289268566 34.0 31.0 35.0 23.5 36.0 90-91 31.18882063584613 34.0 31.0 35.0 23.0 35.0 92-93 30.993406023151067 34.0 31.0 35.0 22.0 35.0 94-95 30.809688463796114 34.0 30.0 35.0 20.0 35.0 96-97 30.649541238776365 34.0 30.0 35.0 20.0 35.0 98-99 30.495828057301146 34.0 30.0 35.0 20.0 35.0 100 28.66985185228811 32.0 26.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33176448298451433 1101 2 -0.3161602028932826 1101 3 -0.3731607781973132 1101 4 -0.24771680197356716 1101 5 -0.3098263149516498 1101 6 -0.3425609098732849 1101 7 -0.27167632328546176 1101 8 -0.38799856213789496 1101 9 -0.46337716614147695 1101 10-11 -0.44526184387189005 1101 12-13 -0.47246238238213323 1101 14-15 -0.7328531332901562 1101 16-17 -0.5901992018375424 1101 18-19 -0.6974571837325385 1101 20-21 -0.721719456957068 1101 22-23 -0.7593368637220834 1101 24-25 -0.7403176826191498 1101 26-27 -0.8613691694407066 1101 28-29 -0.7988783972011149 1101 30-31 -0.6551745475649113 1101 32-33 -0.6274910116660237 1101 34-35 -0.5848710247647091 1101 36-37 -0.557128605556791 1101 38-39 -0.7326044681413464 1101 40-41 -0.74720191851317 1101 42-43 -0.6548402008631413 1101 44-45 -0.6995484909529424 1101 46-47 -0.7368951228288765 1101 48-49 -0.6725934094614203 1101 50-51 -0.7307234727625058 1101 52-53 -0.7656565910928279 1101 54-55 -0.6880880756362089 1101 56-57 -0.6523823373227415 1101 58-59 -0.6976945859884367 1101 60-61 -0.6915211078999519 1101 62-63 -0.8450831928356806 1101 64-65 -0.7252852854922907 1101 66-67 -0.782614031258305 1101 68-69 -0.8417737544112853 1101 70-71 -0.7447567499341972 1101 72-73 -0.8249589161414406 1101 74-75 -0.9389238300442315 1101 76-77 -0.8276277626201534 1101 78-79 -0.7071537260097855 1101 80-81 -0.6333096249391943 1101 82-83 -0.680973087341119 1101 84-85 -0.674796167966722 1101 86-87 -0.8492784861009746 1101 88-89 -0.7896628606436167 1101 90-91 -0.8739216898832716 1101 92-93 -0.9455489586544523 1101 94-95 -0.911606883412361 1101 96-97 -1.0004646836369666 1101 98-99 -0.980298300277024 1101 100 -1.053722386236359 1102 1 -0.3876797766634361 1102 2 -0.3648974777100662 1102 3 -0.3750119822187372 1102 4 -0.26956928493764565 1102 5 -0.28536590291307107 1102 6 -0.3588286281076236 1102 7 -0.36191566117140894 1102 8 -0.39632974917934405 1102 9 -0.5055736697151119 1102 10-11 -0.5117769099056702 1102 12-13 -0.5228308078526425 1102 14-15 -0.631017958655562 1102 16-17 -0.6747592155355733 1102 18-19 -0.6544690720177897 1102 20-21 -0.8323258214570117 1102 22-23 -0.7505843779253851 1102 24-25 -0.7753208819500941 1102 26-27 -0.82915354137252 1102 28-29 -0.8002133897100308 1102 30-31 -0.7440701539854189 1102 32-33 -0.6653706993238657 1102 34-35 -0.7364447549667972 1102 36-37 -0.8039697429445525 1102 38-39 -0.7874548976272848 1102 40-41 -0.6989964938885223 1102 42-43 -0.705310262164474 1102 44-45 -0.702639868246365 1102 46-47 -0.7730125131382906 1102 48-49 -0.6985641023056388 1102 50-51 -0.7213945483522366 1102 52-53 -0.8385481650823934 1102 54-55 -0.760181131370409 1102 56-57 -0.8210989803048605 1102 58-59 -0.780503275391645 1102 60-61 -0.7610259985255183 1102 62-63 -0.84391027108164 1102 64-65 -0.7727308365456338 1102 66-67 -0.8864591762837364 1102 68-69 -0.8645492935545107 1102 70-71 -0.8843220628066035 1102 72-73 -0.8836215153797724 1102 74-75 -0.9976376160837788 1102 76-77 -0.7663794090198195 1102 78-79 -0.5640140240533 1102 80-81 -0.5934732734861683 1102 82-83 -0.6159979584529403 1102 84-85 -0.7242487383245155 1102 86-87 -0.7506464975257536 1102 88-89 -0.7771037539682233 1102 90-91 -0.8359515472711898 1102 92-93 -0.8995945583009686 1102 94-95 -1.005830936084017 1102 96-97 -1.0560138971277624 1102 98-99 -1.0563574082928717 1102 100 -0.9731422866130863 1103 1 -0.3680649305208803 1103 2 -0.3568606793955382 1103 3 -0.38252649276048345 1103 4 -0.254513420539503 1103 5 -0.2360762740723672 1103 6 -0.3276556772251169 1103 7 -0.359939718842071 1103 8 -0.368546427104782 1103 9 -0.5339427944076505 1103 10-11 -0.5248020908595024 1103 12-13 -0.5418501972026561 1103 14-15 -0.6478948276546248 1103 16-17 -0.7310039246793494 1103 18-19 -0.706694931544412 1103 20-21 -0.7404774993300194 1103 22-23 -0.74614157388298 1103 24-25 -0.8351253401762477 1103 26-27 -0.7873628822071836 1103 28-29 -0.7377219177348593 1103 30-31 -0.7264573256574849 1103 32-33 -0.6720961269823036 1103 34-35 -0.8164347920224486 1103 36-37 -0.7838483239369154 1103 38-39 -0.7470007120194637 1103 40-41 -0.7874475191516197 1103 42-43 -0.7125493471936153 1103 44-45 -0.7830173724757756 1103 46-47 -0.7921205816612726 1103 48-49 -0.8124994763806441 1103 50-51 -0.8687691882300328 1103 52-53 -0.8365221398029092 1103 54-55 -0.9523053683732527 1103 56-57 -0.9213895556027509 1103 58-59 -0.9017553774013223 1103 60-61 -0.9434783599345664 1103 62-63 -0.8150110062813312 1103 64-65 -0.8553832656219527 1103 66-67 -0.956973605169388 1103 68-69 -1.0077513131873488 1103 70-71 -1.018735128715278 1103 72-73 -0.9463363130856095 1103 74-75 -0.9436198270949205 1103 76-77 -0.8738096815136558 1103 78-79 -0.7687796706549008 1103 80-81 -0.7314333117965504 1103 82-83 -0.7692682686248808 1103 84-85 -0.7882710470641605 1103 86-87 -0.8460203030699169 1103 88-89 -0.7796666353029984 1103 90-91 -0.9147862570734624 1103 92-93 -0.9517691063504614 1103 94-95 -0.9756996737086574 1103 96-97 -0.9945948984984696 1103 98-99 -1.073753708739467 1103 100 -1.214674275326363 1104 1 -0.39398638697575805 1104 2 -0.3507808083353652 1104 3 -0.40985350506299767 1104 4 -0.2463164258495638 1104 5 -0.28097774551108046 1104 6 -0.4094778394974057 1104 7 -0.40388432794803464 1104 8 -0.4462945311241313 1104 9 -0.5650691613538399 1104 10-11 -0.5174596429086122 1104 12-13 -0.5601446443123521 1104 14-15 -0.7727422784314086 1104 16-17 -0.6332871519867282 1104 18-19 -0.7133136537783216 1104 20-21 -0.7797905102918818 1104 22-23 -0.7736282001546329 1104 24-25 -0.712800044771285 1104 26-27 -0.7258632009422286 1104 28-29 -0.8420027841027249 1104 30-31 -0.8573211058551706 1104 32-33 -0.7759709758296012 1104 34-35 -0.7793915771358755 1104 36-37 -0.7542665486812652 1104 38-39 -0.7965358353074947 1104 40-41 -0.83137598613461 1104 42-43 -0.6740552306483067 1104 44-45 -0.7320523774703815 1104 46-47 -0.8194924879597423 1104 48-49 -0.7365387530820939 1104 50-51 -0.624593948028668 1104 52-53 -0.6960392562036333 1104 54-55 -0.7010088231983005 1104 56-57 -0.5738105454558067 1104 58-59 -0.6711304841939949 1104 60-61 -0.7725563202274159 1104 62-63 -0.7083765937395938 1104 64-65 -0.7797408666313856 1104 66-67 -0.7255857194013942 1104 68-69 -0.7722199128561371 1104 70-71 -0.8164250800178152 1104 72-73 -0.8211915268681054 1104 74-75 -0.7309763552487532 1104 76-77 -0.679709524774875 1104 78-79 -0.5322824949103122 1104 80-81 -0.49658842404386405 1104 82-83 -0.6177228143781477 1104 84-85 -0.5919565442908343 1104 86-87 -0.6056343543566847 1104 88-89 -0.6526020454525785 1104 90-91 -0.6560086799198643 1104 92-93 -0.729676865413051 1104 94-95 -0.8277701371559907 1104 96-97 -0.8085260024389562 1104 98-99 -0.8506610669188 1104 100 -1.0052825170918247 1105 1 -0.44292914002070205 1105 2 -0.4186594016098937 1105 3 -0.4911772605229672 1105 4 -0.2679033753953419 1105 5 -0.3495502775366006 1105 6 -0.4535344207288645 1105 7 -0.37759522378642885 1105 8 -0.4302168804103559 1105 9 -0.5710303489787663 1105 10-11 -0.5539230400786508 1105 12-13 -0.6179682048422919 1105 14-15 -0.9020135788480061 1105 16-17 -0.7784346416083778 1105 18-19 -0.7643469655158626 1105 20-21 -0.8865634060515717 1105 22-23 -0.9328019402189796 1105 24-25 -0.8232232133316018 1105 26-27 -0.9373594684681876 1105 28-29 -0.9412685307374957 1105 30-31 -0.8036497071824797 1105 32-33 -0.8348294099714479 1105 34-35 -0.8949119733894833 1105 36-37 -0.8225242108920625 1105 38-39 -0.8558673043984086 1105 40-41 -0.9831328108156399 1105 42-43 -0.8783274438486188 1105 44-45 -0.9020353936079957 1105 46-47 -0.8221136376858809 1105 48-49 -1.0014981727463734 1105 50-51 -0.9547327885174823 1105 52-53 -0.9807726036817357 1105 54-55 -0.9354095725734553 1105 56-57 -0.9863467373302441 1105 58-59 -0.9965531740499642 1105 60-61 -0.8733021659458657 1105 62-63 -0.8069473874192425 1105 64-65 -0.796144867950666 1105 66-67 -0.8714183343401345 1105 68-69 -0.9526473147044996 1105 70-71 -0.9254390727893878 1105 72-73 -0.8592301656114358 1105 74-75 -0.9116619227588458 1105 76-77 -0.8547221414742765 1105 78-79 -0.8069442274062624 1105 80-81 -0.8065109510186517 1105 82-83 -0.7998504381749463 1105 84-85 -0.8141118741161044 1105 86-87 -0.8974406904550101 1105 88-89 -0.8930659403940098 1105 90-91 -0.9159801016758422 1105 92-93 -0.9890148152683658 1105 94-95 -0.8849029029677347 1105 96-97 -1.0243557005266126 1105 98-99 -0.9939810316894047 1105 100 -1.1639083435160629 1106 1 -0.4089810384954724 1106 2 -0.3945569567420435 1106 3 -0.47186797123613644 1106 4 -0.27665747487965575 1106 5 -0.34457795014858306 1106 6 -0.3427205149103685 1106 7 -0.47204054805872175 1106 8 -0.4125588653546046 1106 9 -0.5088038565106814 1106 10-11 -0.5488041639011456 1106 12-13 -0.6052863739845904 1106 14-15 -0.7250979857350757 1106 16-17 -0.7466575944041196 1106 18-19 -0.7107935995048891 1106 20-21 -0.7370239001768866 1106 22-23 -0.695131517425672 1106 24-25 -0.7164051125827342 1106 26-27 -0.662373909024403 1106 28-29 -0.6673608997390943 1106 30-31 -0.8039196139425968 1106 32-33 -0.8005210564106733 1106 34-35 -0.7412330012673323 1106 36-37 -0.7206547371338061 1106 38-39 -0.7821564106786099 1106 40-41 -0.772385989511541 1106 42-43 -0.7801360179769645 1106 44-45 -0.8534236004221327 1106 46-47 -0.836848660348636 1106 48-49 -0.8317677367899563 1106 50-51 -0.9652439147649687 1106 52-53 -0.8810109365566134 1106 54-55 -0.8745199943436077 1106 56-57 -0.9066731653416369 1106 58-59 -0.9032539218614488 1106 60-61 -0.916011660922571 1106 62-63 -0.8805510997887396 1106 64-65 -0.861972891592309 1106 66-67 -0.8830840056633917 1106 68-69 -0.9573278834061298 1106 70-71 -0.9934517395127358 1106 72-73 -1.0568812583925933 1106 74-75 -1.0670352734604798 1106 76-77 -1.0341032153880398 1106 78-79 -0.8604775799703575 1106 80-81 -0.7394189328268972 1106 82-83 -0.7231739672840227 1106 84-85 -0.8047142369018445 1106 86-87 -0.8145475613620796 1106 88-89 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26-27 0.2638594524590445 2311 28-29 0.6312927281478196 2311 30-31 0.8417264489173135 2311 32-33 0.910421379054533 2311 34-35 0.9566644144037681 2311 36-37 0.972268774547949 2311 38-39 1.0253342361193418 2311 40-41 0.985144574717765 2311 42-43 0.9489648384524614 2311 44-45 0.948835111333338 2311 46-47 0.612865882006524 2311 48-49 0.8351495078470137 2311 50-51 0.9559055942917851 2311 52-53 0.8653939851569703 2311 54-55 0.912784301714467 2311 56-57 0.9686069788549432 2311 58-59 0.990747069982227 2311 60-61 1.031894146527101 2311 62-63 1.0216432696183162 2311 64-65 1.0509619941770225 2311 66-67 1.0563402957331505 2311 68-69 1.099676040234499 2311 70-71 1.114174991271426 2311 72-73 1.0971914654060555 2311 74-75 1.0814498410574416 2311 76-77 1.073246013802887 2311 78-79 0.8032820562524989 2311 80-81 0.8303849545354254 2311 82-83 0.9634229341162914 2311 84-85 0.9586748700208751 2311 86-87 0.9576968811422155 2311 88-89 1.0163024295853766 2311 90-91 1.0609013344493725 2311 92-93 1.1377803987181743 2311 94-95 1.200588123386531 2311 96-97 1.2114073670195005 2311 98-99 1.2462026323768889 2311 100 1.3687185981130305 2312 1 0.25695448093660644 2312 2 0.3363903592301085 2312 3 0.4460409468277149 2312 4 0.1761230679529433 2312 5 0.36656008513913463 2312 6 0.4386513956790381 2312 7 0.3341595650650788 2312 8 0.44164309439088356 2312 9 0.628090113775059 2312 10-11 0.674952381566051 2312 12-13 0.7255028570984337 2312 14-15 0.9090064637642143 2312 16-17 0.9012308510722775 2312 18-19 0.8889020058619721 2312 20-21 0.9178624997840714 2312 22-23 0.9907959638454287 2312 24-25 0.9892342072734692 2312 26-27 1.0148717403130405 2312 28-29 1.0258851115691598 2312 30-31 1.0252457802529662 2312 32-33 0.9062039302412543 2312 34-35 0.9853832245656946 2312 36-37 1.0604399979499917 2312 38-39 0.8666457099885818 2312 40-41 0.9891932254396991 2312 42-43 1.027363117112479 2312 44-45 0.7852184717751882 2312 46-47 0.7694418900102349 2312 48-49 0.9504773887674816 2312 50-51 0.847526871971624 2312 52-53 1.0100172190356815 2312 54-55 0.8608059798674006 2312 56-57 0.9073179812706442 2312 58-59 1.0426412752264227 2312 60-61 1.0312062827503965 2312 62-63 1.1096696897291949 2312 64-65 1.0789591372937224 2312 66-67 1.1115690720948805 2312 68-69 1.175880688328995 2312 70-71 1.0473885525932758 2312 72-73 1.0670948863995378 2312 74-75 1.1235053309008265 2312 76-77 1.036106428154696 2312 78-79 1.0637785295945932 2312 80-81 0.9932865644542801 2312 82-83 1.0110605080270822 2312 84-85 1.010774334154977 2312 86-87 1.048889574973341 2312 88-89 1.0618423392930332 2312 90-91 1.11082155976273 2312 92-93 1.2180437884457511 2312 94-95 1.2317716578542353 2312 96-97 0.8858937152491926 2312 98-99 0.6926738407478403 2312 100 0.8434363209215014 2313 1 0.028655688231914667 2313 2 0.2552567514952031 2313 3 0.3039300626054242 2313 4 0.20698693824090242 2313 5 0.32309827687344495 2313 6 0.21254829673335962 2313 7 0.2159319590425639 2313 8 0.3710603259174263 2313 9 0.5601563623325916 2313 10-11 0.3451960863045471 2313 12-13 0.38855197022350296 2313 14-15 0.4830468753094195 2313 16-17 0.7459864511494132 2313 18-19 0.6928997988117302 2313 20-21 0.6774605398469831 2313 22-23 0.6598689037126206 2313 24-25 0.5230745973768478 2313 26-27 0.7693790822187054 2313 28-29 0.6055712725324796 2313 30-31 0.8477951543554738 2313 32-33 0.9171043940292449 2313 34-35 0.9753025215928943 2313 36-37 1.0368211952772057 2313 38-39 1.0040950652408043 2313 40-41 0.9938628960977027 2313 42-43 1.0582469905792706 2313 44-45 0.9000761826606691 2313 46-47 0.9825712030725029 2313 48-49 1.0436467515440526 2313 50-51 0.9691640592408959 2313 52-53 1.0236393591846564 2313 54-55 1.0010391518975936 2313 56-57 0.667826941955731 2313 58-59 0.795758741085784 2313 60-61 0.5938925130843344 2313 62-63 0.906345592510597 2313 64-65 1.015273881203271 2313 66-67 0.9596874109993792 2313 68-69 1.0826291450049155 2313 70-71 1.0642414894195866 2313 72-73 1.0422195416772695 2313 74-75 1.1399164733809073 2313 76-77 1.1180414933034797 2313 78-79 0.9850491339773413 2313 80-81 0.8807062598206485 2313 82-83 0.9180057324257938 2313 84-85 0.9579715710163619 2313 86-87 0.9711964893627858 2313 88-89 0.9759057515377556 2313 90-91 0.8665439716100494 2313 92-93 1.0620588151687151 2313 94-95 1.1306263847187097 2313 96-97 1.2821249956487293 2313 98-99 1.2223029335287912 2313 100 1.2614822379721637 2314 1 -0.35191035575380525 2314 2 0.03201470408205864 2314 3 0.2795142607805303 2314 4 0.2287098273749919 2314 5 0.31348014777007194 2314 6 0.40310722644410646 2314 7 0.42760048536603534 2314 8 0.45764293455831506 2314 9 0.458808087515429 2314 10-11 0.5606001959209195 2314 12-13 0.42025501217460004 2314 14-15 0.7235955576373314 2314 16-17 0.7882842004318462 2314 18-19 0.7996909771492184 2314 20-21 0.8591510664336184 2314 22-23 0.9102718941215215 2314 24-25 0.7690309736215113 2314 26-27 0.710911044558145 2314 28-29 0.7743893447331871 2314 30-31 0.8034901211381396 2314 32-33 0.8467106250629897 2314 34-35 0.8757446602252941 2314 36-37 0.8253245163378793 2314 38-39 0.5476648313125025 2314 40-41 0.6477002645313306 2314 42-43 0.7783128180550776 2314 44-45 0.8321879444687141 2314 46-47 0.8371455044824714 2314 48-49 0.8397214173935552 2314 50-51 0.764233794087275 2314 52-53 0.8197779780297552 2314 54-55 0.7531921479433947 2314 56-57 0.8015288942179737 2314 58-59 0.8535868521640495 2314 60-61 0.9357970241587026 2314 62-63 0.8771643309955053 2314 64-65 0.8976699171415561 2314 66-67 0.6353784591136247 2314 68-69 0.6502889504522358 2314 70-71 0.7814189455331189 2314 72-73 0.9155245769618716 2314 74-75 1.0212052366965523 2314 76-77 0.8625733379049834 2314 78-79 0.7281077700643905 2314 80-81 0.8464522974151478 2314 82-83 0.8026863482091322 2314 84-85 0.8818291597021322 2314 86-87 0.9608254913167364 2314 88-89 0.9063257697171885 2314 90-91 1.0166908940322834 2314 92-93 0.9159438220337144 2314 94-95 0.9453752471582568 2314 96-97 0.8928731649296289 2314 98-99 1.0495880914868003 2314 100 1.1925425072463653 2315 1 0.4530668197963834 2315 2 0.4959806956728059 2315 3 0.488398001624077 2315 4 0.31638282734749623 2315 5 0.38327268648160384 2315 6 0.45595710624306207 2315 7 0.4122942091829742 2315 8 0.24994043529558496 2315 9 0.5471023792973355 2315 10-11 0.5998390203031576 2315 12-13 0.4323439703501748 2315 14-15 0.6801953109632635 2315 16-17 0.8036135218121885 2315 18-19 0.8092853990493367 2315 20-21 0.5299422103925409 2315 22-23 0.8636704317249411 2315 24-25 0.9020394717102391 2315 26-27 0.8867706462913674 2315 28-29 0.8897332468284205 2315 30-31 0.9558990451346929 2315 32-33 0.9340482386408624 2315 34-35 0.9581468635546244 2315 36-37 0.8772598306795842 2315 38-39 0.9616394401416883 2315 40-41 0.9679981532090736 2315 42-43 1.0687463535076276 2315 44-45 1.1161668178874322 2315 46-47 1.095119439268096 2315 48-49 0.9477516352961644 2315 50-51 1.0049008516097615 2315 52-53 1.0649670400015623 2315 54-55 0.9046984933399216 2315 56-57 0.9444424181784825 2315 58-59 0.9506980315527827 2315 60-61 1.0278237393350977 2315 62-63 1.104384423052899 2315 64-65 1.1558845475411061 2315 66-67 1.1709149440578983 2315 68-69 1.1970026256472224 2315 70-71 1.111179017293722 2315 72-73 1.1908700377678727 2315 74-75 0.59236593648221 2315 76-77 0.6165297106568275 2315 78-79 0.9097570118582254 2315 80-81 0.9209275279614744 2315 82-83 0.856485969290258 2315 84-85 0.9542377686018888 2315 86-87 0.9692179926599387 2315 88-89 0.9225915875314605 2315 90-91 1.012692764964445 2315 92-93 1.0160311326004887 2315 94-95 1.1091956292844074 2315 96-97 1.202053000915157 2315 98-99 1.2004759785489192 2315 100 1.086382334918369 2316 1 0.18247351661696598 2316 2 0.003736216987647367 2316 3 0.19503352028802823 2316 4 -0.014296298520257267 2316 5 0.2445966975049032 2316 6 0.3337214454617481 2316 7 0.3852771827126915 2316 8 0.4117289050118842 2316 9 0.5738660981332302 2316 10-11 0.5836450043278418 2316 12-13 0.38594706895053577 2316 14-15 0.465430752677662 2316 16-17 0.7643044478393648 2316 18-19 0.7307233985081112 2316 20-21 0.6752620020764581 2316 22-23 0.7453271667441683 2316 24-25 0.3964100040796694 2316 26-27 0.6951071551746253 2316 28-29 0.8996116923000912 2316 30-31 0.5200123855738994 2316 32-33 0.6568080032267574 2316 34-35 0.8005690809905133 2316 36-37 0.8057070511989721 2316 38-39 0.7495029485628777 2316 40-41 0.7376073120473166 2316 42-43 0.7431232553858962 2316 44-45 0.6674216663538743 2316 46-47 0.8397566257860944 2316 48-49 0.875720038114217 2316 50-51 0.8655939867151403 2316 52-53 0.8054852161129631 2316 54-55 0.7472790280473092 2316 56-57 0.8090988236768766 2316 58-59 0.7295842143208731 2316 60-61 0.8362938974498704 2316 62-63 0.8648139414922511 2316 64-65 0.92698833187616 2316 66-67 0.9389910026205541 2316 68-69 0.9607093116065002 2316 70-71 0.9066465410827362 2316 72-73 0.8731397919895159 2316 74-75 0.9398370021599476 2316 76-77 0.7819250528154242 2316 78-79 0.536433472104946 2316 80-81 0.5308897343151457 2316 82-83 0.5160554199477367 2316 84-85 0.6017592748986864 2316 86-87 0.6310580075344028 2316 88-89 0.6473203771590939 2316 90-91 0.5359514130454066 2316 92-93 0.4178075996585271 2316 94-95 0.4654987737231444 2316 96-97 0.6644296377040391 2316 98-99 0.8463042264001324 2316 100 0.38169338233439376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 10.0 12 31.0 13 161.0 14 634.0 15 2056.0 16 4693.0 17 9957.0 18 17405.0 19 26380.0 20 38746.0 21 56005.0 22 68308.0 23 67689.0 24 65433.0 25 67204.0 26 78169.0 27 95543.0 28 117894.0 29 145813.0 30 180102.0 31 226696.0 32 288580.0 33 385191.0 34 568099.0 35 775777.0 36 1189948.0 37 1680555.0 38 1266778.0 39 216815.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.255607025210765 22.210641622543275 13.946654404033678 22.587096948212285 2 15.266733283730453 20.32180360792259 41.58023789751835 22.83122521082861 3 18.52586515476034 27.419190818624244 29.23557246503422 24.819371561581193 4 12.270029574523047 16.7844222952286 39.70680517774761 31.238742952500743 5 12.597627045219111 39.19843574234509 34.31357267051318 13.890364541922615 6 29.225233077924294 38.43953781630836 18.085970040644263 14.249259065123088 7 26.97157231453227 32.68218592351532 23.022505387328117 17.323736374624293 8 26.89841290407863 35.45343120413942 19.494966747091393 18.15318914469056 9 27.12925528733076 15.203108869464055 21.854431368072987 35.813204475132196 10-11 26.183225344069154 26.48486192571406 26.64176608499943 20.69014664521736 12-13 25.940023948120867 24.088900167694433 27.782758426177345 22.188317458007354 14-15 23.448695936806118 25.260066958730683 25.658087997836315 25.633149106626878 16-17 21.48837408359598 27.839894595582727 27.177877741424783 23.49385357939651 18-19 20.919583780773582 27.737787907759582 29.87890483641192 21.46372347505492 20-21 22.498075925003842 26.928549398944558 29.248066714441617 21.325307961609987 22-23 22.880147526039877 26.619642087064182 28.90954148744517 21.59066889945077 24-25 22.129108294593124 26.486328370398294 29.86555445617121 21.519008878837376 26-27 21.924933592019944 26.829661567971723 28.86476382631421 22.380641013694127 28-29 21.110559094655788 27.59797106904443 29.31890563581171 21.972564200488073 30-31 22.58429105986658 26.805152549116613 29.265492134666754 21.345064256350053 32-33 22.85037149241049 27.036869511560507 28.37584700145238 21.736911994576623 34-35 22.550251918244864 27.073246932375895 29.31770944711255 21.058791702266692 36-37 22.065815685993933 26.740294009696285 29.05619985008416 22.137690454225627 38-39 22.442238262303967 26.79778873865303 29.52037432704605 21.239598671996955 40-41 22.004057016008318 25.786643991282876 30.1254379405991 22.083861052109715 42-43 22.009198002717167 26.16273511240262 29.124460838272192 22.703606046608023 44-45 21.830078465910397 27.13189567626282 29.151466962974038 21.886558894852747 46-47 21.968884183375735 26.73449255465119 28.39206017960364 22.90456308236943 48-49 22.8727478332343 26.76282848325092 28.354874470251634 22.009549213263146 50-51 22.47087517571559 26.493339545512228 28.323733317940324 22.712051960831857 52-53 21.720483632785268 27.277591721089667 28.00352912872851 22.998395517396556 54-55 21.69865554434976 27.085699476236258 28.064436749457943 23.15120822995604 56-57 22.845023848092566 26.514605492488663 27.464524301784156 23.175846357634615 58-59 21.674412833324748 27.19225504189095 28.4395888987398 22.693743226044504 60-61 22.181435737629414 27.843258874730616 27.599768099037075 22.375537288602896 62-63 22.59267051476148 26.91626385570986 27.243372637735824 23.24769299179283 64-65 21.58778933779703 27.046085220901254 27.61677106906793 23.749354372233782 66-67 22.06796833093067 26.742038468469225 27.604568274775374 23.58542492582473 68-69 21.518958166471887 26.817602391594846 27.949381331458724 23.714058110474543 70-71 21.60882391590142 26.29287251102731 28.1919613671486 23.906342205922666 72-73 22.139625195066326 26.719287545373625 28.059984711566337 23.081102547993716 74-75 22.358341483468642 27.05461310751239 27.62620271775918 22.96084269125978 76-77 21.957553834720446 26.710364913065764 27.407883764488737 23.924197487725046 78-79 21.73677910181471 26.73097048700081 27.58549462891296 23.94675578227152 80-81 21.33491733217042 27.31271115876312 27.972877182851875 23.379494326214587 82-83 22.51684953981066 26.187732740273002 29.059039787191686 22.23637793272465 84-85 22.703675996192572 26.813415985619876 27.45421389559376 23.02869412259379 86-87 21.001216044453326 27.64762708947892 28.03854622579664 23.31261064027111 88-89 21.981443855124525 26.207481722463825 28.029561008429248 23.781513413982406 90-91 22.003466984265142 26.058897092555856 28.2783261498961 23.659309773282907 92-93 22.377207885593737 26.441757279489014 27.818232476487452 23.362802358429793 94-95 20.990952830550498 26.484296942769586 28.452554031760975 24.07219619491894 96-97 21.262974699552647 26.385168260529866 28.077841751317028 24.274015288600456 98-99 20.496526122350346 26.89230573474535 28.033676859343366 24.57749128356094 100 21.20635076908897 26.461808440818395 27.178509367333348 25.153331422759283 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3103.0 1 2014.5 2 1312.0 3 1701.5 4 1340.5 5 1120.5 6 1702.0 7 3183.5 8 4897.0 9 5560.0 10 4800.5 11 3983.0 12 4263.5 13 5118.5 14 6127.5 15 7419.0 16 8812.0 17 10140.5 18 11381.5 19 12308.5 20 13046.0 21 13835.0 22 14673.0 23 15673.5 24 16931.0 25 18691.5 26 22049.5 27 25684.5 28 30766.5 29 43881.0 30 64159.5 31 84099.0 32 113410.5 33 146671.0 34 171313.5 35 197641.0 36 222839.5 37 259309.5 38 272153.0 39 250614.0 40 225119.5 41 198973.5 42 189979.0 43 198473.0 44 218627.5 45 264212.5 46 305060.5 47 327337.5 48 346671.0 49 347251.0 50 336054.5 51 344356.5 52 346378.0 53 323134.5 54 302268.5 55 261083.5 56 223563.0 57 183920.0 58 143578.0 59 133469.5 60 105585.5 61 66857.0 62 47462.0 63 39961.0 64 28198.5 65 12187.5 66 6067.5 67 3787.5 68 2893.5 69 2368.0 70 1965.5 71 1623.5 72 1327.0 73 1354.5 74 1126.5 75 814.0 76 538.5 77 297.5 78 198.5 79 137.5 80 90.5 81 61.5 82 38.0 83 21.0 84 14.5 85 7.5 86 4.5 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04438083758197072 2 0.0 3 0.0 4 0.035232443164454494 5 0.0 6 0.0 7 9.161482249300945E-4 8 0.0 9 0.011334062325563742 10-11 4.3844236478797385E-4 12-13 2.6175663569431275E-5 14-15 1.1124657017008293E-4 16-17 2.4212488801723928E-4 18-19 0.0 20-21 6.543915892357819E-6 22-23 0.0 24-25 5.889524303122037E-4 26-27 4.8424977603447856E-4 28-29 1.43966149631872E-4 30-31 0.002552127198019549 32-33 0.015201516617947212 34-35 0.026129856158184767 36-37 0.03140425236742517 38-39 0.02220350662277008 40-41 0.00123025618776327 42-43 5.889524303122036E-5 44-45 0.011392957568594961 46-47 0.01857163330251149 48-49 0.0034682754229496443 50-51 0.011170464428254796 52-53 0.01098069086737642 54-55 0.010339387109925354 56-57 4.188106171109004E-4 58-59 0.00876230337986712 60-61 0.004639636367681693 62-63 0.0014069419168569312 64-65 0.036253294043662315 66-67 0.06173530252850366 68-69 0.06032181669575437 70-71 0.05333945843860858 72-73 0.046317836686108636 74-75 0.0363187332025859 76-77 0.03230731276057055 78-79 0.015312763188117296 80-81 0.0014003980009645732 82-83 0.03944018108324057 84-85 0.052796313419542884 86-87 0.058253939273769303 88-89 0.07099494351618997 90-91 0.04958325071639519 92-93 0.04006185309301456 94-95 0.013846926028229144 96-97 0.00374966380632103 98-99 0.02186976691225983 100 0.032614876807511364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7640685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.39691520168245 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81971938978323 49.96927066374492 2 13.504023388273186 17.122268512557834 3 3.9312092095854965 7.476796107004945 4 1.5547908850840746 3.942757835920955 5 0.7180083467895018 2.275975713775712 6 0.4031148149218796 1.5333741442886588 7 0.24848297443819345 1.1027137841663985 8 0.15036283542448128 0.7626031941512299 9 0.10613239901680593 0.6055620030557614 >10 0.49238697766132683 5.5117791182370075 >50 0.037785171028864195 1.6673434105336098 >100 0.029377732041412617 3.6316001352758436 >500 0.003106305315658029 1.3328562843840812 >1k 0.001365680400536726 1.602085109487731 >5k 2.6778047062234867E-5 0.10286034562380392 >10k+ 1.0711218824893947E-4 1.3601536377915777 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29424 0.3850963624334729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24431 0.3197488183323877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15974 0.20906502492904758 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 10291 0.13468687689650863 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.543915892357819E-6 0.0 12-13 0.0 0.0 0.0 3.9263495354146915E-5 0.0 14-15 0.0 0.0 0.0 3.9263495354146915E-5 0.0 16-17 0.0 0.0 0.0 5.235132713886255E-5 0.0 18-19 0.0 0.0 0.0 7.198307481593602E-5 0.0 20-21 0.0 0.0 0.0 1.5051006552422983E-4 0.0 22-23 0.0 0.0 0.0 4.3844236478797385E-4 0.0 24-25 0.0 0.0 0.0 9.488678043918837E-4 0.0 26-27 0.0 0.0 0.0 0.0021202287491239335 0.0 28-29 0.0 0.0 0.0 0.005516521097257641 0.0 30-31 0.0 0.0 0.0 0.01914095398514662 0.0 32-33 0.0 0.0 0.0 0.050532118520787074 0.0 34-35 0.0 0.0 0.0 0.0945399528968934 0.0 36-37 0.0 0.0 0.0 0.1670661727318951 0.0 38-39 0.0 0.0 0.0 0.2783650942291169 0.0 40-41 6.543915892357819E-6 0.0 0.0 0.4012205711922426 0.0 42-43 1.3087831784715637E-5 0.0 0.0 0.5156736601495808 0.0 44-45 1.3087831784715637E-5 0.0 0.0 0.6466305049874455 0.0 46-47 1.3087831784715637E-5 0.0 0.0 0.8003470892989306 0.0 48-49 1.3087831784715637E-5 0.0 0.0 0.9723670063613407 0.0 50-51 1.3087831784715637E-5 0.0 0.0 1.1547197666177835 0.0 52-53 1.3087831784715637E-5 0.0 0.0 1.3313662322160904 0.0 54-55 1.3087831784715637E-5 0.0 0.0 1.518227750522368 0.0 56-57 1.3087831784715637E-5 0.0 0.0 1.7315855842768024 0.0 58-59 1.3087831784715637E-5 0.0 0.0 1.9843835991144774 0.0 60-61 1.3087831784715637E-5 0.0 0.0 2.240355413159946 0.0 62-63 1.3087831784715637E-5 0.0 0.0 2.5057373782586243 0.0 64-65 1.3087831784715637E-5 0.0 0.0 2.762442110884037 0.0 66-67 1.3087831784715637E-5 0.0 0.0 3.050335146652427 0.0 68-69 1.3087831784715637E-5 0.0 0.0 3.3695146443021797 0.0 70-71 1.3087831784715637E-5 0.0 0.0 3.7135937419223537 0.0 72-73 1.3087831784715637E-5 0.0 0.0 4.057528873392896 0.0 74-75 1.3087831784715637E-5 0.0 0.0 4.384449823543308 0.0 76-77 1.3087831784715637E-5 0.0 0.0 4.752100106207754 0.0 78-79 1.3087831784715637E-5 0.0 0.0 5.143164519935058 0.0 80-81 1.3087831784715637E-5 0.0 0.0 5.5559416465932046 0.0 82-83 1.3087831784715637E-5 0.0 0.0 5.97670889455592 0.0 84-85 1.3087831784715637E-5 0.0 0.0 6.380134765403888 0.0 86-87 1.3087831784715637E-5 0.0 0.0 6.802407114021845 0.0 88 1.3087831784715637E-5 0.0 0.0 7.145170884547655 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16560 0.0 48.437477 1 GTATCAA 29790 0.0 40.68883 1 ACCTGGG 3565 0.0 39.026146 3 GTACTAG 8075 0.0 38.429455 1 TACCTGG 3785 0.0 37.87145 2 GGGCACC 8295 0.0 34.23132 94 CAACGCA 34310 0.0 33.483948 5 ATCAACG 34630 0.0 33.199184 3 TAGGCAT 9765 0.0 33.056965 5 GTACACG 515 0.0 32.86685 1 GTGGTAT 4820 0.0 31.995552 1 CCTGGGG 3710 0.0 31.926413 4 TATCAAC 38645 0.0 31.10894 2 AACGCAG 39290 0.0 30.076996 6 CTAGGCA 11335 0.0 29.856194 4 CTGTGCG 10695 0.0 29.435383 9 TCTAACG 4110 0.0 29.387949 2 TAACGCC 4170 0.0 29.193567 4 GCATCGT 9865 0.0 29.149424 8 CTAACGC 4130 0.0 29.021084 3 >>END_MODULE