FastQCFastQC Report
Mon 6 Jun 2016
ERR1211227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3507351
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140930.40181322029075506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112430.3205553136826055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70940.20226090858884668No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.13443194023067553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.048.4892771
TACCTGG36300.046.544522
GTATCAA114650.041.2135851
GTACCTG43500.039.814321
ACCTGGG45300.037.04243
GTGGTAT24950.035.4623951
ATCAACG136300.033.5886233
CAACGCA137600.033.3396075
TATCAAC143250.032.8209842
TCAACGC144600.031.8881864
GTACTAG14650.031.1612511
AACGCAG149600.031.1365996
CCTGGGG43500.030.6872634
GTACATG134950.030.4105571
TACATGG136750.029.494042
TAACGCA3850.029.300814
ACATGGG136900.028.6346383
TGGTATC30800.028.1156332
CTGGGGG31450.027.9479565
ACGCAGA165350.027.8580687
Produced by FastQC (version 0.11.5)