FastQCFastQC Report
Mon 6 Jun 2016
ERR1211227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3507351
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131010.37352976648188335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111610.318217366895985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73740.21024414151877016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37280.1062910441526953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62250.052.795691
TACCTGG38450.050.1158032
GTACCTG45450.043.034831
ACCTGGG47400.039.9634933
GTATCAA108450.039.799151
CCTGGGG42300.034.1141664
ATCAACG134150.031.4295753
TATCAAC138750.030.8922142
CTGGGGG29550.030.848385
CAACGCA135650.030.7942815
GTACTAG16550.030.3980981
GTGGTAT26300.030.2128451
GTACATG125400.029.1329271
TCAACGC144950.029.0553784
AACGCAG147950.028.9011126
TACATGG127000.028.5693782
TGGGGGG79650.027.608846
TGGTATC27750.027.606532
ACATGGG128600.027.1571753
CATGGGG104950.025.7974514
Produced by FastQC (version 0.11.5)