FastQCFastQC Report
Mon 6 Jun 2016
ERR1211226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5167244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211210.408747874108519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169430.3278923929274484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100640.19476533331888335No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.10158219739574907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97950.050.6907961
TACCTGG47500.045.0806852
GTATCAA170650.039.3824771
ACCTGGG57800.037.004233
GTACCTG60050.035.8949741
ATCAACG202600.032.2277953
CAACGCA204200.031.9292355
TATCAAC214450.031.0090752
AACGCAG220050.030.0783026
GTGGTAT40100.029.8105831
TCAACGC221200.029.7091244
GTACATG205500.029.3142811
TACATGG205500.028.7869832
GTACTAG27900.028.1704251
ACATGGG208300.027.4869063
CCTGGGG56450.026.8138854
ACGCAGA242150.026.8090387
CGCAGAG246850.026.240458
CATGGGG155550.026.049844
TGGTATC46400.025.7625262
Produced by FastQC (version 0.11.5)