##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5167244 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.301064939066162 31.0 31.0 34.0 28.0 34.0 2 31.301236597304094 31.0 31.0 34.0 28.0 34.0 3 31.358271643452486 31.0 31.0 34.0 28.0 34.0 4 34.959103924645326 37.0 35.0 37.0 32.0 37.0 5 35.017664348732126 37.0 35.0 37.0 32.0 37.0 6 35.08259683498592 37.0 35.0 37.0 32.0 37.0 7 34.99832483234777 37.0 35.0 37.0 32.0 37.0 8 34.99739280746177 37.0 35.0 37.0 32.0 37.0 9 36.543974312031715 39.0 37.0 39.0 32.0 39.0 10-11 36.48157422022262 39.0 37.0 39.0 32.0 39.0 12-13 36.370263238972264 39.0 37.0 39.0 32.0 39.0 14-15 37.53035738200093 40.0 37.0 41.0 32.0 41.0 16-17 37.49843669081623 40.0 37.0 41.0 32.0 41.0 18-19 37.52210443323365 40.0 37.0 41.0 32.0 41.0 20-21 37.487827844011235 40.0 37.0 41.0 31.0 41.0 22-23 37.37442570933364 40.0 37.0 41.0 31.0 41.0 24-25 37.27817023929971 40.0 36.5 41.0 31.0 41.0 26-27 37.12608520131815 40.0 36.0 41.0 30.5 41.0 28-29 36.99696279099652 39.5 36.0 41.0 30.0 41.0 30-31 36.82823938641179 39.0 36.0 41.0 30.0 41.0 32-33 36.612963699798186 39.0 35.0 41.0 30.0 41.0 34-35 36.453660500645995 39.0 35.0 41.0 29.5 41.0 36-37 36.336933479433135 39.0 35.0 41.0 29.0 41.0 38-39 36.18906219640489 39.0 35.0 40.0 28.5 41.0 40-41 36.02251693552695 39.0 35.0 40.0 28.0 41.0 42-43 35.81378593695208 38.0 35.0 40.0 27.0 41.0 44-45 35.59434661881653 38.0 34.0 40.0 26.5 41.0 46-47 35.357056875967146 38.0 34.0 40.0 26.0 41.0 48-49 35.14343603669577 38.0 34.0 40.0 24.5 41.0 50-51 34.462181483978696 37.5 32.5 39.5 24.0 40.5 52-53 34.57587439261626 38.0 33.0 39.5 24.0 40.5 54-55 35.065274351278944 38.0 34.0 40.0 25.0 41.0 56-57 35.03261167848857 38.0 34.0 40.0 25.0 41.0 58-59 34.82920643964171 38.0 34.0 40.0 25.0 41.0 60-61 34.60319640411794 37.0 33.0 40.0 25.0 41.0 62-63 34.29847835712809 37.0 33.0 40.0 24.0 41.0 64-65 34.05342064357711 36.0 33.0 39.5 24.0 41.0 66-67 33.88067081794473 36.0 33.0 39.0 23.5 41.0 68-69 33.58004005616921 35.0 32.5 39.0 23.0 41.0 70-71 33.262845048540385 35.0 32.0 38.5 23.0 40.5 72-73 32.91748841355276 35.0 32.0 37.5 22.5 40.0 74-75 32.58381634387693 35.0 31.5 37.0 22.0 39.5 76-77 32.24793690795325 35.0 31.0 36.5 21.0 39.0 78-79 31.90857892524526 35.0 31.0 36.0 20.0 39.0 80-81 31.615222931218266 35.0 31.0 36.0 20.0 37.5 82-83 31.325050355663485 34.5 30.5 35.0 20.0 37.0 84-85 31.071942606155236 34.0 30.0 35.0 19.5 37.0 86-87 30.83646282234785 34.0 30.0 35.0 19.0 36.0 88-89 30.617514868661125 34.0 30.0 35.0 18.0 36.0 90-91 30.392460371524937 34.0 29.0 35.0 18.0 36.0 92-93 30.190437784629484 34.0 29.0 35.0 17.5 35.0 94-95 30.01038425899764 34.0 29.0 35.0 17.0 35.0 96-97 29.82832279644623 34.0 29.0 35.0 16.0 35.0 98-99 29.58551105773213 34.0 29.0 35.0 15.0 35.0 100 28.449694072894566 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3870949494814191 1101 2 -0.3779387742140443 1101 3 -0.42806398631229925 1101 4 -0.4090680853717643 1101 5 -0.27808962141041604 1101 6 -0.2765129805542088 1101 7 -0.29560359563475913 1101 8 -0.22713522930774843 1101 9 -0.5257805034252527 1101 10-11 -0.5264514201212478 1101 12-13 -0.46391994433686534 1101 14-15 -0.583606225900084 1101 16-17 -0.6065344086109405 1101 18-19 -0.5566204088623365 1101 20-21 -0.6975713980683906 1101 22-23 -0.7260846498647453 1101 24-25 -0.855728605412402 1101 26-27 -0.8331590733386207 1101 28-29 -0.8209481551362785 1101 30-31 -0.9327594879973446 1101 32-33 -0.8907879943971793 1101 34-35 -0.8083223612010357 1101 36-37 -0.9305221196115028 1101 38-39 -0.9341590131104027 1101 40-41 -0.9430158463432505 1101 42-43 -0.9846416744346129 1101 44-45 -0.9724537368904222 1101 46-47 -1.0472216054556114 1101 48-49 -1.1857804620154226 1101 50-51 -1.1355221873487764 1101 52-53 -1.0342699142080463 1101 54-55 -0.9082083384483397 1101 56-57 -0.9135251258806534 1101 58-59 -0.8416739480117954 1101 60-61 -0.8178031734595805 1101 62-63 -0.9779024380451062 1101 64-65 -0.9161530560488771 1101 66-67 -0.8210023052966804 1101 68-69 -0.7828123767265112 1101 70-71 -0.8960859496664568 1101 72-73 -0.9251270882945875 1101 74-75 -0.950660080948218 1101 76-77 -0.9181111198844008 1101 78-79 -0.9895045489027048 1101 80-81 -1.0462282296508292 1101 82-83 -1.043714871778235 1101 84-85 -1.1532147519453808 1101 86-87 -1.0706307117643483 1101 88-89 -1.1538840801571943 1101 90-91 -1.3143466282749294 1101 92-93 -1.4386260550925911 1101 94-95 -1.5643652582336962 1101 96-97 -1.4994081584073982 1101 98-99 -1.5965889169669616 1101 100 -1.716417024278016 1102 1 -0.5586156304721825 1102 2 -0.4444623810108759 1102 3 -0.4221104735571508 1102 4 -0.3488399108645055 1102 5 -0.39028741664328237 1102 6 -0.41140929130688875 1102 7 -0.3942587377101532 1102 8 -0.45179170990297735 1102 9 -0.5451635657934943 1102 10-11 -0.5257582964330823 1102 12-13 -0.5208847669860575 1102 14-15 -0.6911070780132889 1102 16-17 -0.8077440026766425 1102 18-19 -0.7770668900913691 1102 20-21 -0.8327883734831758 1102 22-23 -0.796830072815979 1102 24-25 -0.7866168150661608 1102 26-27 -0.8067472472197394 1102 28-29 -0.8667848627765125 1102 30-31 -0.8388220835195739 1102 32-33 -1.0091757916143393 1102 34-35 -1.0055272150465484 1102 36-37 -0.9853466456777298 1102 38-39 -1.0522368114678073 1102 40-41 -0.9909890906792853 1102 42-43 -1.0589447179635272 1102 44-45 -1.2618801054855169 1102 46-47 -1.1844129052915733 1102 48-49 -1.2230001988755035 1102 50-51 -1.2483717475110367 1102 52-53 -1.0581421283810428 1102 54-55 -0.9480136499565219 1102 56-57 -0.9158871023413013 1102 58-59 -0.9108628544110147 1102 60-61 -0.8542683239862754 1102 62-63 -0.9842257241926191 1102 64-65 -1.1047429341564055 1102 66-67 -1.0650144547965468 1102 68-69 -0.9562235136350523 1102 70-71 -1.0766872952763578 1102 72-73 -1.0247233531459479 1102 74-75 -1.0344867651896728 1102 76-77 -1.1875027379142438 1102 78-79 -1.0177528113360736 1102 80-81 -1.074801914154552 1102 82-83 -1.116464544783149 1102 84-85 -1.107773829978079 1102 86-87 -1.1782458184077953 1102 88-89 -1.250185916532125 1102 90-91 -1.2520931790388552 1102 92-93 -1.333842296945722 1102 94-95 -1.327747502327714 1102 96-97 -1.3378567798562777 1102 98-99 -1.3961262359955384 1102 100 -1.3587205429338844 1103 1 -0.5083854946850082 1103 2 -0.4727838781171201 1103 3 -0.6136517479870172 1103 4 -0.3621487540321411 1103 5 -0.4401745842200171 1103 6 -0.4819036714521445 1103 7 -0.4930290231326495 1103 8 -0.43544939233206037 1103 9 -0.5911704275108249 1103 10-11 -0.6464431768883685 1103 12-13 -0.609436967571277 1103 14-15 -0.817301878073998 1103 16-17 -0.835777756513707 1103 18-19 -0.7274709954737943 1103 20-21 -0.9046346984287936 1103 22-23 -0.8441929237701231 1103 24-25 -0.8516980479398129 1103 26-27 -0.930036229998386 1103 28-29 -0.9547137789381352 1103 30-31 -1.030091939496458 1103 32-33 -1.0890665006467017 1103 34-35 -1.046930405062703 1103 36-37 -1.055598800847612 1103 38-39 -1.012627880528541 1103 40-41 -1.162391277675205 1103 42-43 -1.2546410993253474 1103 44-45 -1.2660038864188365 1103 46-47 -1.1570818539028167 1103 48-49 -1.285442106061133 1103 50-51 -1.2635271332885054 1103 52-53 -1.18386542068761 1103 54-55 -0.9506897432485886 1103 56-57 -0.9082437057441552 1103 58-59 -0.9590393724424331 1103 60-61 -0.9594190388073258 1103 62-63 -0.9465398124795783 1103 64-65 -0.9237636687319508 1103 66-67 -1.0211690869852035 1103 68-69 -1.0588696192692595 1103 70-71 -1.0043502698873041 1103 72-73 -1.123055736404396 1103 74-75 -0.9935680251242793 1103 76-77 -1.0461208584013875 1103 78-79 -1.0444562397239565 1103 80-81 -0.9161433051818442 1103 82-83 -0.9113726434215117 1103 84-85 -1.0349435304132903 1103 86-87 -1.1458645895344581 1103 88-89 -1.257657755488843 1103 90-91 -1.2700296663855006 1103 92-93 -1.2652689505760648 1103 94-95 -1.3748859238268203 1103 96-97 -1.2759302809440136 1103 98-99 -1.333869416928625 1103 100 -1.475216196120666 1104 1 -0.5119768404438076 1104 2 -0.5648350745178554 1104 3 -0.5639418422676599 1104 4 -0.3581607019628379 1104 5 -0.3878429931195768 1104 6 -0.42696141045541935 1104 7 -0.48876528622768234 1104 8 -0.37793858822549 1104 9 -0.555198915340803 1104 10-11 -0.5380303385492411 1104 12-13 -0.6243991963670652 1104 14-15 -0.8382260891568833 1104 16-17 -0.9711748111516982 1104 18-19 -0.9153380790616694 1104 20-21 -0.7546601382443541 1104 22-23 -0.8321911065128447 1104 24-25 -0.9107749116109858 1104 26-27 -0.8737273045976295 1104 28-29 -0.8800257404446725 1104 30-31 -0.944332892210852 1104 32-33 -0.8562054041236635 1104 34-35 -1.000636416063891 1104 36-37 -0.977582522938242 1104 38-39 -1.0394161253986027 1104 40-41 -1.0146365609637016 1104 42-43 -0.9320083418934644 1104 44-45 -0.9183804177200798 1104 46-47 -0.9601019872318588 1104 48-49 -1.0129245944174627 1104 50-51 -1.0623936845605257 1104 52-53 -0.8400106619386349 1104 54-55 -0.7659987663001289 1104 56-57 -0.7629597325004767 1104 58-59 -0.8149467834224282 1104 60-61 -0.7967474949603357 1104 62-63 -0.6830428664483037 1104 64-65 -0.6437688013524365 1104 66-67 -0.7581858495759022 1104 68-69 -0.9564858237023728 1104 70-71 -0.9188014843891565 1104 72-73 -0.8513539660671299 1104 74-75 -0.8728657760599994 1104 76-77 -0.6815391252905059 1104 78-79 -0.80958124463627 1104 80-81 -0.9033421954164105 1104 82-83 -0.9569715404439982 1104 84-85 -0.9188499964433205 1104 86-87 -1.0667110372490143 1104 88-89 -1.073389907128604 1104 90-91 -1.16892902593926 1104 92-93 -1.169380941735099 1104 94-95 -1.0730294871515689 1104 96-97 -1.1343850227827303 1104 98-99 -1.2427587047566924 1104 100 -1.3050555367635184 1105 1 -0.5307475283437029 1105 2 -0.6008954188841926 1105 3 -0.6333890688048882 1105 4 -0.3962150146046284 1105 5 -0.3704239232774782 1105 6 -0.4118853417743509 1105 7 -0.3986853226167142 1105 8 -0.4514960896983382 1105 9 -0.7634269529107698 1105 10-11 -0.6738859356261528 1105 12-13 -0.7111075583135786 1105 14-15 -0.897549224775176 1105 16-17 -0.8927273117851371 1105 18-19 -0.8226720588722998 1105 20-21 -0.8106494054831259 1105 22-23 -0.8917868503751762 1105 24-25 -0.8376274648204429 1105 26-27 -0.9146374815446237 1105 28-29 -0.9303782297581193 1105 30-31 -0.8860707841377149 1105 32-33 -0.9381593721691672 1105 34-35 -1.0188604872170757 1105 36-37 -1.0098304789659949 1105 38-39 -1.0630901504980201 1105 40-41 -1.1389572697127122 1105 42-43 -1.1691995498612044 1105 44-45 -1.212348648947291 1105 46-47 -1.1850214023482621 1105 48-49 -1.1006742328927928 1105 50-51 -1.167038401362639 1105 52-53 -1.1542810955073506 1105 54-55 -0.8789891718754532 1105 56-57 -0.8789322467503098 1105 58-59 -0.8720957595901737 1105 60-61 -0.9450275130782089 1105 62-63 -0.9300569623359536 1105 64-65 -0.821217090907048 1105 66-67 -0.8991082456218109 1105 68-69 -0.9178690492821175 1105 70-71 -0.8709901696804394 1105 72-73 -0.802123305002084 1105 74-75 -0.8657377964017812 1105 76-77 -0.855502171138955 1105 78-79 -1.002355585884601 1105 80-81 -0.9123346562766024 1105 82-83 -0.9483737314212917 1105 84-85 -1.1356896245287373 1105 86-87 -0.9534934949858958 1105 88-89 -1.0442766142671651 1105 90-91 -1.1129914945716273 1105 92-93 -1.0581825724025862 1105 94-95 -1.1487465559061825 1105 96-97 -1.1691975893226534 1105 98-99 -1.1910464906250162 1105 100 -1.2075422634172632 1106 1 -0.6427140196385324 1106 2 -0.5755231501272249 1106 3 -0.599332741480513 1106 4 -0.5593452064369302 1106 5 -0.43813087384297944 1106 6 -0.4681283634060023 1106 7 -0.453555025325322 1106 8 -0.4825448508858514 1106 9 -0.627236627639526 1106 10-11 -0.6402872814095559 1106 12-13 -0.7154246516839606 1106 14-15 -0.8959553082899276 1106 16-17 -0.8994610454574143 1106 18-19 -0.9641250602528544 1106 20-21 -0.8459033480307738 1106 22-23 -0.875879558321337 1106 24-25 -0.8279252051565535 1106 26-27 -0.9163311186370251 1106 28-29 -1.0095597335236732 1106 30-31 -0.9928491535557598 1106 32-33 -1.0464913734361545 1106 34-35 -1.0300143074174386 1106 36-37 -1.1167008254913 1106 38-39 -1.098804831288831 1106 40-41 -1.1032919998259274 1106 42-43 -1.1525200050167541 1106 44-45 -1.154240895749595 1106 46-47 -1.1109023038170136 1106 48-49 -1.0290169681255676 1106 50-51 -1.09345639290526 1106 52-53 -1.0453980843036135 1106 54-55 -1.011748942328616 1106 56-57 -0.9157856599006919 1106 58-59 -0.9650677222207804 1106 60-61 -0.9954174628265804 1106 62-63 -1.0255840725134249 1106 64-65 -0.9710108787395768 1106 66-67 -1.035399835590951 1106 68-69 -0.9449679571905136 1106 70-71 -0.9594247640242983 1106 72-73 -0.8939439981780701 1106 74-75 -0.7561053019844728 1106 76-77 -0.9230219410316352 1106 78-79 -0.9317492246731938 1106 80-81 -0.8843678541355615 1106 82-83 -0.8782241675647029 1106 84-85 -0.891578625329636 1106 86-87 -0.9018218116375145 1106 88-89 -0.9023307022451235 1106 90-91 -0.9335132764836978 1106 92-93 -1.067539785398985 1106 94-95 -1.1032366704067336 1106 96-97 -0.963261399264713 1106 98-99 -0.9343205004526247 1106 100 -1.134948473040442 1107 1 -0.6122462598707799 1107 2 -0.6043104987470933 1107 3 -0.5521211922418416 1107 4 -0.4763224918064708 1107 5 -0.3998299013570801 1107 6 -0.48128189074298433 1107 7 -0.40410187567761824 1107 8 -0.43188119637018474 1107 9 -0.6451453453501728 1107 10-11 -0.6012828764759988 1107 12-13 -0.682401141408377 1107 14-15 -0.8645212288720927 1107 16-17 -0.827769982178026 1107 18-19 -0.827704352862078 1107 20-21 -0.8835266384315545 1107 22-23 -0.9857504311717094 1107 24-25 -0.9075621687187763 1107 26-27 -0.9226684536617356 1107 28-29 -0.9214375174167699 1107 30-31 -0.964872303542478 1107 32-33 -0.9706555612070176 1107 34-35 -0.9779284804861632 1107 36-37 -1.0651642099027399 1107 38-39 -1.1389262744092008 1107 40-41 -1.0611745252450575 1107 42-43 -1.0680186444635922 1107 44-45 -1.0658457820138594 1107 46-47 -0.95465803332673 1107 48-49 -0.8894478194727711 1107 50-51 -1.0206770470916027 1107 52-53 -0.9425863782190689 1107 54-55 -0.8145656384918141 1107 56-57 -0.8256273706276502 1107 58-59 -0.8758542831519733 1107 60-61 -0.8616564154670172 1107 62-63 -0.7506071937877294 1107 64-65 -0.749006538841364 1107 66-67 -0.8134972647577783 1107 68-69 -0.7621759061350062 1107 70-71 -0.697002105919232 1107 72-73 -0.6435488782155403 1107 74-75 -0.6019948738789687 1107 76-77 -0.6959203914608736 1107 78-79 -0.6539654483269643 1107 80-81 -0.761064437635401 1107 82-83 -0.7864730529696651 1107 84-85 -0.8259836306639166 1107 86-87 -0.8109660140136548 1107 88-89 -0.7834563048598184 1107 90-91 -0.8549115596544858 1107 92-93 -0.8309027533420945 1107 94-95 -0.8935801590546113 1107 96-97 -0.8471886888892293 1107 98-99 -0.7833502760876172 1107 100 -0.7602169600278401 1108 1 -0.7462237410946848 1108 2 -0.6542212678878414 1108 3 -0.6724348167700889 1108 4 -0.6102301347325749 1108 5 -0.5499427877977467 1108 6 -0.6676431524297257 1108 7 -0.5859378162795394 1108 8 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1.2115304799479674 2311 62-63 1.2023959934141573 2311 64-65 1.1180911294558413 2311 66-67 1.1239843822810869 2311 68-69 1.0944415914504404 2311 70-71 1.110952063150215 2311 72-73 1.0886945569006983 2311 74-75 1.1452606749629268 2311 76-77 1.1267724523532152 2311 78-79 1.1950208683085535 2311 80-81 1.1384260913625006 2311 82-83 1.2621353237897246 2311 84-85 1.2839004283285576 2311 86-87 1.2632726093443978 2311 88-89 1.3098740430927833 2311 90-91 1.358028965404575 2311 92-93 1.3357984909946694 2311 94-95 1.3519716110878477 2311 96-97 1.3834278931307118 2311 98-99 1.4462495613127722 2311 100 1.503272599241246 2312 1 0.6906488640267696 2312 2 0.6863435436447105 2312 3 0.7033090092963192 2312 4 0.3685825119141768 2312 5 0.4448375163834797 2312 6 0.5510077818894175 2312 7 0.40534220937836807 2312 8 0.5520059142092677 2312 9 0.7583096622918433 2312 10-11 0.7228561369001909 2312 12-13 0.861202712342596 2312 14-15 1.028654704172908 2312 16-17 1.0703417462455747 2312 18-19 1.0639391624686212 2312 20-21 1.1105178063742471 2312 22-23 1.1516607048792054 2312 24-25 1.122016235586294 2312 26-27 1.1828279368980432 2312 28-29 1.1953222712323281 2312 30-31 1.1737485798010496 2312 32-33 1.2434197904417417 2312 34-35 1.2284256655890644 2312 36-37 1.2556979768470171 2312 38-39 1.2423450189336052 2312 40-41 1.2273815241943709 2312 42-43 1.2937911285423667 2312 44-45 1.3067968343657554 2312 46-47 1.262636823477152 2312 48-49 1.3505058219566948 2312 50-51 1.4269882802107787 2312 52-53 1.2478905672511686 2312 54-55 1.19375513883071 2312 56-57 1.138276550184699 2312 58-59 1.1233064925647227 2312 60-61 1.0692445666035937 2312 62-63 1.1041466177767418 2312 64-65 1.1205510169805777 2312 66-67 1.0913629150629234 2312 68-69 1.1397361308149812 2312 70-71 1.1054824004292385 2312 72-73 1.1691505887320375 2312 74-75 1.118639415981015 2312 76-77 1.0902097265851083 2312 78-79 1.1387159345772488 2312 80-81 1.2055446195974788 2312 82-83 1.1963582855459727 2312 84-85 1.2497988777162163 2312 86-87 1.2526217409411196 2312 88-89 1.1753226800453582 2312 90-91 1.2568753033958124 2312 92-93 1.3524353483110296 2312 94-95 1.3959776610152552 2312 96-97 1.4023058099264851 2312 98-99 1.1133070724521694 2312 100 1.4211365550707953 2313 1 0.644601802863356 2313 2 0.6258132650092847 2313 3 0.6841818680717893 2313 4 0.5442379807578988 2313 5 0.4184685691114254 2313 6 0.5756696688820071 2313 7 0.6393933619932994 2313 8 0.6413021592922661 2313 9 0.7894638872837945 2313 10-11 0.8659378428081723 2313 12-13 0.9113408304011728 2313 14-15 1.062452706117604 2313 16-17 1.086790383691202 2313 18-19 1.0716777989040054 2313 20-21 1.0851472158919293 2313 22-23 1.14064886776697 2313 24-25 1.0825055918140762 2313 26-27 1.1477132752320358 2313 28-29 1.1984455940844754 2313 30-31 1.2279749923936265 2313 32-33 1.248342791597203 2313 34-35 1.231823448754767 2313 36-37 1.2773971130961428 2313 38-39 1.3198475997681811 2313 40-41 1.29488814596872 2313 42-43 1.4178580424008942 2313 44-45 1.3888261753768774 2313 46-47 1.4552030727905034 2313 48-49 1.4104201729754493 2313 50-51 1.4262384570018938 2313 52-53 1.35872431099245 2313 54-55 1.215015572663475 2313 56-57 1.1636081124499 2313 58-59 1.150779279360954 2313 60-61 1.2169932422487193 2313 62-63 1.1800572498681206 2313 64-65 1.2145025509592031 2313 66-67 1.1768308827227827 2313 68-69 1.1535602883737468 2313 70-71 1.1631784570402743 2313 72-73 1.2222084436244174 2313 74-75 1.176276358062978 2313 76-77 1.0307762631840305 2313 78-79 1.165536843599277 2313 80-81 1.2228865257213961 2313 82-83 1.2833351732646783 2313 84-85 1.3184393303646118 2313 86-87 1.365905541192415 2313 88-89 1.332142081607433 2313 90-91 1.354295494835334 2313 92-93 1.453194958170922 2313 94-95 1.503274122351673 2313 96-97 1.5861922468909846 2313 98-99 1.593444472296845 2313 100 1.758055038581869 2314 1 0.5180884823620353 2314 2 0.5016663020398404 2314 3 0.5690660701176178 2314 4 0.4488298292721282 2314 5 0.4239256669384943 2314 6 0.4980794498872285 2314 7 0.5122739841999646 2314 8 0.5070235588562682 2314 9 0.6918036025978722 2314 10-11 0.6761208508451872 2314 12-13 0.7423116035540858 2314 14-15 0.8961670656958063 2314 16-17 0.9663436359252557 2314 18-19 0.8453078056359544 2314 20-21 0.8750706704002624 2314 22-23 0.9695960323302018 2314 24-25 0.9149598382858812 2314 26-27 0.9360759423458802 2314 28-29 1.0029698649322327 2314 30-31 1.025674283473677 2314 32-33 0.9922858641345087 2314 34-35 0.992631166674137 2314 36-37 1.0085988563538706 2314 38-39 1.0133025303708223 2314 40-41 1.0643241299141266 2314 42-43 1.1619014409636108 2314 44-45 1.2325208099926854 2314 46-47 1.2445333050547518 2314 48-49 1.2191518332868654 2314 50-51 1.2512893870795878 2314 52-53 1.2191751386327923 2314 54-55 1.151500692513963 2314 56-57 1.1714125810515696 2314 58-59 1.1201712595977114 2314 60-61 1.0936675704516858 2314 62-63 1.1071067867735138 2314 64-65 1.165780405291592 2314 66-67 1.1448665249673198 2314 68-69 1.1294041965714143 2314 70-71 1.1735067213651007 2314 72-73 1.2303554333272118 2314 74-75 1.2132773708925626 2314 76-77 1.1946622053281928 2314 78-79 1.203711238028049 2314 80-81 1.2197624446804447 2314 82-83 1.1987233476439165 2314 84-85 1.2955415289399639 2314 86-87 1.337958553433861 2314 88-89 1.332848692765932 2314 90-91 1.4060614455341671 2314 92-93 1.4475474611419692 2314 94-95 1.4332873401726083 2314 96-97 1.4347617694089756 2314 98-99 1.5256732168499205 2314 100 1.6673680582114407 2315 1 0.5096797851300323 2315 2 0.4842580679557358 2315 3 0.4957510018571405 2315 4 0.26313128223815596 2315 5 0.3286301313667437 2315 6 0.4105436383234178 2315 7 0.4554100686985265 2315 8 0.4346064272977017 2315 9 0.576142702885825 2315 10-11 0.507313502676773 2315 12-13 0.5332953141138432 2315 14-15 0.7132430080996599 2315 16-17 0.7310812880697455 2315 18-19 0.7072119096560812 2315 20-21 0.8211482899838671 2315 22-23 0.6002270175006714 2315 24-25 0.7493898999711988 2315 26-27 0.8427502513066756 2315 28-29 0.8880503708745522 2315 30-31 0.9303548625733455 2315 32-33 0.934684915636744 2315 34-35 0.9136022981330214 2315 36-37 0.9466811844560894 2315 38-39 0.9933460982211813 2315 40-41 1.058570571303477 2315 42-43 1.1552401134893344 2315 44-45 1.00767615562021 2315 46-47 1.0670977168351854 2315 48-49 1.0675197045525806 2315 50-51 0.9487138611650963 2315 52-53 0.9664431372768121 2315 54-55 0.8990953009103322 2315 56-57 0.8863169957578947 2315 58-59 0.8327893302111349 2315 60-61 0.9098142092829704 2315 62-63 0.8794525100460362 2315 64-65 0.9167023284240017 2315 66-67 0.9361195710417789 2315 68-69 0.8608876326900159 2315 70-71 0.9215720855761731 2315 72-73 0.6271308027561631 2315 74-75 0.7805845081118221 2315 76-77 0.8756922448317468 2315 78-79 0.9232875724545977 2315 80-81 0.619871144409661 2315 82-83 0.5648614484180605 2315 84-85 0.889928689036573 2315 86-87 1.0032144691852274 2315 88-89 1.0775800695142514 2315 90-91 1.1087165538249373 2315 92-93 1.2007279253393008 2315 94-95 1.2166902771227264 2315 96-97 0.9744335561801698 2315 98-99 0.5045376835590325 2315 100 1.0841097677781804 2316 1 0.2761990592128498 2316 2 0.2650511621142009 2316 3 0.31461879723631725 2316 4 0.20354127177948556 2316 5 0.13566126593628525 2316 6 0.18164819023991896 2316 7 0.13602060180395625 2316 8 0.20294086283215762 2316 9 0.23299447927210792 2316 10-11 0.25319188378031043 2316 12-13 0.07123922517573078 2316 14-15 0.1211958624623648 2316 16-17 0.26324456183979805 2316 18-19 0.3054288955269584 2316 20-21 0.3693787258886374 2316 22-23 0.3404226646365984 2316 24-25 0.4275638038363425 2316 26-27 0.4505272734191976 2316 28-29 0.4344556505567567 2316 30-31 0.31646275693305626 2316 32-33 0.43770454794123026 2316 34-35 0.3998099307155414 2316 36-37 0.5334844321912726 2316 38-39 0.6692130524747597 2316 40-41 0.7161966143247085 2316 42-43 0.6270791018691 2316 44-45 0.6222477542469633 2316 46-47 0.47037772554174495 2316 48-49 0.4595814915196925 2316 50-51 0.5667987858249646 2316 52-53 0.6122728645422342 2316 54-55 0.28757959087196383 2316 56-57 0.1862970592186386 2316 58-59 0.20067433421333192 2316 60-61 0.3419123226992866 2316 62-63 0.4756998193451807 2316 64-65 0.44873180855608297 2316 66-67 0.33891164058479006 2316 68-69 0.32191576196319005 2316 70-71 0.10675800270165325 2316 72-73 -0.003843009984940693 2316 74-75 0.2380359858967509 2316 76-77 0.41163530876107046 2316 78-79 0.40907431412848183 2316 80-81 0.5973600177814156 2316 82-83 0.6485419359851967 2316 84-85 0.7203900527099947 2316 86-87 0.7435532106487202 2316 88-89 0.6608705086223985 2316 90-91 0.5332262131400505 2316 92-93 0.6032488110032119 2316 94-95 0.7117987579937655 2316 96-97 0.695895494351003 2316 98-99 0.524436288122434 2316 100 0.43153267414751895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 14.0 1 333.0 2 109.0 3 113.0 4 84.0 5 65.0 6 86.0 7 467.0 8 1427.0 9 2569.0 10 3774.0 11 5881.0 12 8817.0 13 11530.0 14 14086.0 15 17009.0 16 20651.0 17 25566.0 18 31546.0 19 37766.0 20 44864.0 21 53092.0 22 58825.0 23 60605.0 24 59973.0 25 61165.0 26 66163.0 27 75255.0 28 88156.0 29 103257.0 30 124558.0 31 152248.0 32 190304.0 33 247269.0 34 344945.0 35 471360.0 36 692208.0 37 982334.0 38 876004.0 39 232495.0 40 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.26472023422251 18.93848560894755 12.567211172042011 23.229582984787932 2 16.082875091875984 19.742663921965534 41.08715278726493 23.087308198893556 3 19.536995672798867 26.179199084544173 28.91873393945635 25.365071303200608 4 12.7149360679155 16.193263420318132 38.08037286941441 33.01142764235196 5 13.170570058587655 37.41457095981709 34.10386901512811 15.310989966467142 6 30.194644007831766 36.428185837143886 18.833317068788183 14.543853086236167 7 27.608224529834892 32.17766164842238 22.357869984176148 17.85624383756658 8 26.271175611148262 35.05150068173202 20.25913561568669 18.418188091433024 9 27.114758865458306 15.930846259777235 21.02245281729393 35.93194205747052 10-11 25.60215687756891 25.972623367586955 27.704574294595346 20.720645460248786 12-13 25.730782377179747 23.812512215159895 27.95000891059311 22.50669649706725 14-15 23.107337754179458 25.10232215398272 26.275787166822713 25.51455292501511 16-17 22.06236283826132 27.651041954991012 27.217302131912962 23.06929307483471 18-19 21.538959584116242 27.14229927429388 29.4932445020394 21.825496639550476 20-21 22.815951134593277 26.478601592411277 29.124709977303393 21.580737295692057 22-23 22.740969467343003 26.317342147699346 28.90122756455455 22.040460820403098 24-25 22.22012841035254 26.115518601227848 29.52868036716491 22.13567262125469 26-27 22.053865178139194 26.427834090714207 28.99793835744906 22.520362373697537 28-29 21.81742604863135 26.99486626596217 29.095317893257626 22.092389792148857 30-31 22.740511237900428 26.476525922010314 29.140895180223136 21.642067659866125 32-33 22.665336584787976 26.5485478595257 28.689815795209462 22.09629976047686 34-35 22.62578994512788 26.750650030656576 28.94376369806832 21.679796326147226 36-37 22.404199327728165 26.637702003362328 28.687047104517653 22.271051564391854 38-39 22.474341547167565 26.81680331656886 28.83881860542093 21.870036530842647 40-41 22.45981929364961 26.13031480512361 29.191325870636415 22.21854003059037 42-43 22.332418463938968 26.43267240432226 28.583310788279924 22.651598343458843 44-45 22.091766280923096 26.995938632745624 28.73299958907153 22.17929549725975 46-47 22.11607316333887 26.614496110200196 28.239711302612687 23.029719423848242 48-49 22.50552102247768 26.62141496541218 28.246203431891193 22.626860580218953 50-51 22.44661496229996 26.765232047776905 28.174982459302143 22.61317053062099 52-53 22.156785019914302 27.16873263336823 27.919547089375506 22.754935257341963 54-55 22.07706120078407 26.96557365147739 28.168129129056457 22.78923601868209 56-57 22.62136320806861 26.834215469727706 27.690951601852436 22.853469720351242 58-59 22.09239898030167 27.066816493990288 28.205816508898156 22.634968016809882 60-61 22.417340794078893 27.312714658228 27.867024798971595 22.402919748721512 62-63 22.546220824131627 26.961336811374593 27.700699650760185 22.791742713733594 64-65 22.086684355534235 26.876209479707647 27.863266873288435 23.17383929146968 66-67 22.231979050695436 26.63474789923287 27.769724522530048 23.36354852754165 68-69 21.870979464127636 26.748654501398132 28.048042327243344 23.332323707230888 70-71 21.997835119427567 26.492459799569463 28.13495790370173 23.37474717730124 72-73 22.209490128534664 26.780749539011513 27.895892497717323 23.1138678347365 74-75 22.377074742110885 26.916240650436723 27.59762464616407 23.109059961288324 76-77 22.17806768361688 26.643691681610765 27.630906942503273 23.547333692269085 78-79 21.992504780085113 26.67732693072274 27.757419052128192 23.57274923706396 80-81 21.84269983948884 26.94130038041067 27.77920269392388 23.436797086176604 82-83 22.44030329460853 26.520150795614107 28.282936503788665 22.756609405988698 84-85 22.513307637428383 26.808855448340896 27.53392862050772 23.143908293723005 86-87 21.811318366324258 27.051058681642388 27.8939993475269 23.243623604506457 88-89 22.111183105502537 26.337333457858357 27.894667312519267 23.656816124119835 90-91 22.38899662525003 26.27218027779044 27.898049237920503 23.44077385903903 92-93 22.395916556133077 26.436156750743166 27.90103610419789 23.266890588925865 94-95 21.738674700649266 26.425993681252553 28.079905452041697 23.75542616605648 96-97 21.858445563017693 26.343342065741123 27.892382228396777 23.905830142844408 98-99 21.620754437569147 26.54576656048829 27.669040919812577 24.164438082129994 100 22.04174268024122 26.329566467095546 27.362641276842215 24.266049575821025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2248.0 1 1566.5 2 2374.5 3 3505.5 4 2302.5 5 1520.0 6 2074.0 7 3692.5 8 5791.5 9 6680.5 10 5682.5 11 4503.0 12 4480.0 13 4948.0 14 5472.0 15 6140.5 16 6712.0 17 7294.5 18 7881.5 19 8342.5 20 9045.0 21 9935.5 22 11144.5 23 12819.5 24 14715.0 25 16948.0 26 20458.5 27 25012.5 28 30385.0 29 38359.5 30 48939.5 31 59731.5 32 73269.5 33 88064.5 34 100983.5 35 113514.0 36 125940.5 37 141652.5 38 149758.0 39 149195.5 40 147159.0 41 144453.0 42 145792.0 43 152200.5 44 162113.5 45 177876.5 46 193041.0 47 203172.5 48 212700.5 49 217029.0 50 216321.5 51 218433.0 52 216721.5 53 208122.0 54 196952.5 55 177059.0 56 157593.0 57 136761.5 58 115434.0 59 101312.5 60 83105.0 61 63254.5 62 49109.5 63 38983.0 64 28787.0 65 18971.0 66 13129.5 67 9568.5 68 7186.0 69 5482.0 70 4299.0 71 3309.5 72 2460.5 73 1924.0 74 1574.0 75 1149.5 76 815.5 77 544.0 78 346.0 79 242.5 80 168.0 81 127.5 82 108.5 83 75.5 84 60.5 85 53.5 86 36.0 87 28.0 88 25.0 89 25.5 90 21.5 91 17.0 92 13.5 93 8.0 94 6.5 95 6.5 96 3.5 97 3.0 98 3.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05658722522102692 2 0.1307273277592465 3 0.016565890830779424 4 0.015946605192245614 5 0.01873339056564776 6 0.051013654474222625 7 0.05589052887767637 8 0.0475688781098783 9 0.03980845495200149 10-11 0.03869567607026105 12-13 0.039547193823245036 14-15 0.018636627184626855 16-17 0.01737870323135505 18-19 0.017349674217048777 20-21 0.020388044381105287 22-23 0.024839159908067048 24-25 0.02375541004063288 26-27 0.018868859299077032 28-29 0.017930254503174224 30-31 0.016314306040125067 32-33 0.022178166929992083 34-35 0.022091079887073265 36-37 0.022816805244730072 38-39 0.02054286579073874 40-41 0.023968289478878874 42-43 0.032154471513247684 44-45 0.04086317580512939 46-47 0.04624321978989186 48-49 0.03845376761770878 50-51 0.02710342302395629 52-53 0.03844409127960669 54-55 0.03617015182561536 56-57 0.037602249864724796 58-59 0.0423243028585451 60-61 0.022894215949546798 62-63 0.02534232948937577 64-65 0.024829483569964953 66-67 0.03156421488902014 68-69 0.03803768507931888 70-71 0.039924571009226584 72-73 0.043659637516633626 74-75 0.026338992313891117 76-77 0.020233222971471832 78-79 0.01970102437585684 80-81 0.0241521399028186 82-83 0.020242899309573924 84-85 0.0128114716471682 86-87 0.014466125462625726 88-89 0.01831730802725786 90-91 0.020968624667230734 92-93 0.019671995361550565 94-95 0.0230006556686698 96-97 0.023968289478878874 98-99 0.027267920771691833 100 0.029029014306272357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5167244.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.24412565289676 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56371650893846 57.29371951171123 2 12.36253921885273 17.367915165559104 3 3.22302696632931 6.791961336165321 4 1.1722146482185145 3.2936477216651 5 0.5269696912577614 1.85082626039892 6 0.2886524910018089 1.216568510877163 7 0.17871280218928243 0.8787467172935666 8 0.13069114643913232 0.7344228249753232 9 0.09459205622406565 0.5980082654852228 >10 0.42213035659118797 5.175107015593946 >50 0.022489739069722396 1.0752634993281698 >100 0.013073400945155432 1.7100780317082738 >500 7.521944040432516E-4 0.3537232127098593 >1k 3.134139563459559E-4 0.48489115251344106 >5k 3.1341395634539334E-5 0.11555930349787998 >10k+ 9.402418690361801E-5 1.0595614705174257 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21121 0.408747874108519 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16943 0.3278923929274484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10064 0.19476533331888335 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5249 0.10158219739574907 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.870535240836314E-5 0.0 0.0 0.0 0.0 7 3.870535240836314E-5 0.0 0.0 1.935267620418157E-5 0.0 8 3.870535240836314E-5 0.0 0.0 1.935267620418157E-5 0.0 9 3.870535240836314E-5 0.0 0.0 3.870535240836314E-5 0.0 10-11 3.870535240836314E-5 0.0 0.0 5.805802861254471E-5 0.0 12-13 3.870535240836314E-5 0.0 0.0 8.708704291881706E-5 0.0 14-15 3.870535240836314E-5 9.676338102090785E-6 0.0 1.2579239532718023E-4 0.0 16-17 3.870535240836314E-5 1.935267620418157E-5 0.0 1.35468733429271E-4 0.0 18-19 3.870535240836314E-5 1.935267620418157E-5 0.0 2.128794382459973E-4 2.9029014306272356E-5 20-21 3.870535240836314E-5 1.935267620418157E-5 0.0 2.902901430627236E-4 3.870535240836314E-5 22-23 3.870535240836314E-5 1.935267620418157E-5 0.0 5.128459194108116E-4 3.870535240836314E-5 24-25 3.870535240836314E-5 1.935267620418157E-5 0.0 0.0012095422627613481 3.870535240836314E-5 26-27 3.870535240836314E-5 1.935267620418157E-5 0.0 0.002399731849318515 3.870535240836314E-5 28-29 3.870535240836314E-5 1.935267620418157E-5 0.0 0.00591224258037747 5.805802861254471E-5 30-31 3.870535240836314E-5 3.870535240836314E-5 0.0 0.01971070071395893 5.805802861254471E-5 32-33 3.870535240836314E-5 3.870535240836314E-5 0.0 0.05206837532735052 5.805802861254471E-5 34-35 3.870535240836314E-5 5.805802861254471E-5 0.0 0.09939534498467656 5.805802861254471E-5 36-37 3.870535240836314E-5 6.77343667146355E-5 0.0 0.1753933044384976 5.805802861254471E-5 38-39 3.870535240836314E-5 7.741070481672628E-5 0.0 0.2883451991042033 5.805802861254471E-5 40-41 3.870535240836314E-5 7.741070481672628E-5 0.0 0.41543422373706373 5.805802861254471E-5 42-43 3.870535240836314E-5 7.741070481672628E-5 0.0 0.532392122377035 5.805802861254471E-5 44-45 3.870535240836314E-5 7.741070481672628E-5 0.0 0.6702218823032162 5.805802861254471E-5 46-47 3.870535240836314E-5 7.741070481672628E-5 0.0 0.8281590728055419 5.805802861254471E-5 48-49 3.870535240836314E-5 7.741070481672628E-5 0.0 1.0013364958186608 5.805802861254471E-5 50-51 3.870535240836314E-5 7.741070481672628E-5 0.0 1.1847127791913832 5.805802861254471E-5 52-53 3.870535240836314E-5 7.741070481672628E-5 0.0 1.3631734828082436 8.708704291881706E-5 54-55 3.870535240836314E-5 7.741070481672628E-5 0.0 1.5626415164447431 1.1611605722508942E-4 56-57 4.838169051045393E-5 7.741070481672628E-5 0.0 1.780601032194338 1.1611605722508942E-4 58-59 5.805802861254471E-5 7.741070481672628E-5 0.0 2.037227194999888 1.1611605722508942E-4 60-61 5.805802861254471E-5 7.741070481672628E-5 0.0 2.2972884578316797 1.35468733429271E-4 62-63 6.77343667146355E-5 7.741070481672628E-5 0.0 2.5595075440602377 1.7417408583763415E-4 64-65 7.741070481672628E-5 7.741070481672628E-5 0.0 2.817769008005041 1.7417408583763415E-4 66-67 8.708704291881706E-5 7.741070481672628E-5 0.0 3.1089203451588507 1.7417408583763415E-4 68-69 9.676338102090785E-5 7.741070481672628E-5 0.0 3.446847100698167 1.7417408583763415E-4 70-71 1.35468733429271E-4 9.676338102090785E-5 0.0 3.7959887320978067 1.935267620418157E-4 72-73 1.451450715313618E-4 1.1611605722508942E-4 0.0 4.129077318586078 2.4190845255226963E-4 74-75 1.5482140963345257E-4 1.1611605722508942E-4 0.0 4.457337412361406 2.515847906543604E-4 76-77 1.5482140963345257E-4 1.1611605722508942E-4 0.0 4.823673896568462 2.515847906543604E-4 78-79 1.5482140963345257E-4 1.1611605722508942E-4 0.0 5.221971325526722 2.515847906543604E-4 80-81 1.5482140963345257E-4 1.1611605722508942E-4 0.0 5.6349090540334466 2.70937466858542E-4 82-83 1.5482140963345257E-4 1.1611605722508942E-4 0.0 6.05682642429891 2.902901430627236E-4 84-85 1.5482140963345257E-4 1.1611605722508942E-4 0.0 6.478414799068904 2.902901430627236E-4 86-87 1.5482140963345257E-4 1.1611605722508942E-4 0.0 6.9200525463864295 2.902901430627236E-4 88 1.5482140963345257E-4 1.1611605722508942E-4 0.0 7.271322972168529 2.902901430627236E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9795 0.0 50.690796 1 TACCTGG 4750 0.0 45.080685 2 GTATCAA 17065 0.0 39.382477 1 ACCTGGG 5780 0.0 37.00423 3 GTACCTG 6005 0.0 35.894974 1 ATCAACG 20260 0.0 32.227795 3 CAACGCA 20420 0.0 31.929235 5 TATCAAC 21445 0.0 31.009075 2 AACGCAG 22005 0.0 30.078302 6 GTGGTAT 4010 0.0 29.810583 1 TCAACGC 22120 0.0 29.709124 4 GTACATG 20550 0.0 29.314281 1 TACATGG 20550 0.0 28.786983 2 GTACTAG 2790 0.0 28.170425 1 ACATGGG 20830 0.0 27.486906 3 CCTGGGG 5645 0.0 26.813885 4 ACGCAGA 24215 0.0 26.809038 7 CGCAGAG 24685 0.0 26.24045 8 CATGGGG 15555 0.0 26.04984 4 TGGTATC 4640 0.0 25.762526 2 >>END_MODULE