FastQCFastQC Report
Mon 6 Jun 2016
ERR1211226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5167244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192250.3720552000253907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162430.3143455195845213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100670.19482339134749588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95600.052.1337931
TACCTGG48700.048.6388932
GTATCAA168250.039.4594421
ACCTGGG59250.038.7922253
GTACCTG62150.038.507741
ATCAACG199200.032.5150223
CAACGCA200350.032.3871655
CCTGGGG52950.031.7790744
GTGGTAT34750.031.526261
TATCAAC211900.031.0734392
AACGCAG219500.030.0111146
TCAACGC218200.029.7483654
GTACTAG30350.028.3506951
GTACATG204850.027.8876321
CTGGGGG41900.027.137515
TACATGG206750.027.0510162
ACATGGG204750.026.5832943
ACGCAGA245450.026.3596447
CGCAGAG250050.025.8556828
TGGGGGG119950.025.3046826
Produced by FastQC (version 0.11.5)