##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211226_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5167244 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.178486636202976 33.0 31.0 34.0 30.0 34.0 2 32.31395885311396 34.0 31.0 34.0 31.0 34.0 3 32.391587469064746 34.0 31.0 34.0 30.0 34.0 4 36.000252358897704 37.0 35.0 37.0 35.0 37.0 5 35.89291390149178 37.0 35.0 37.0 35.0 37.0 6 35.92370652518054 37.0 35.0 37.0 35.0 37.0 7 35.86469847369313 37.0 35.0 37.0 35.0 37.0 8 35.85041445691359 37.0 35.0 37.0 35.0 37.0 9 37.46268784675158 39.0 37.0 39.0 35.0 39.0 10-11 37.44027038398032 39.0 37.0 39.0 34.5 39.0 12-13 37.34435513399406 39.0 37.0 39.0 34.0 39.0 14-15 38.574946915609175 40.0 38.0 41.0 34.0 41.0 16-17 38.52660451103141 40.0 38.0 41.0 34.0 41.0 18-19 38.552433270037184 40.0 38.0 41.0 34.0 41.0 20-21 38.518177291414915 40.0 38.0 41.0 34.0 41.0 22-23 38.3907036323425 40.0 38.0 41.0 34.0 41.0 24-25 38.3301231759135 40.0 38.0 41.0 34.0 41.0 26-27 38.19196616223272 40.0 38.0 41.0 34.0 41.0 28-29 38.16031427972048 40.0 38.0 41.0 33.5 41.0 30-31 38.157808398442185 40.0 37.5 41.0 34.0 41.0 32-33 38.008971126581216 40.0 37.0 41.0 33.0 41.0 34-35 37.865232607556365 40.0 37.0 41.0 33.0 41.0 36-37 37.71863221477445 40.0 37.0 41.0 33.0 41.0 38-39 37.62866791272098 40.0 37.0 41.0 33.0 41.0 40-41 37.5285424880265 40.0 36.0 41.0 32.5 41.0 42-43 37.37877502978377 40.0 36.0 41.0 32.0 41.0 44-45 37.237015321900806 40.0 36.0 41.0 31.5 41.0 46-47 36.99766200318777 39.5 36.0 41.0 31.0 41.0 48-49 36.762063297185115 39.0 35.0 41.0 30.5 41.0 50-51 36.560034227143134 39.0 35.0 41.0 30.0 41.0 52-53 36.360887544695004 39.0 35.0 40.5 30.0 41.0 54-55 36.12890788590591 38.5 35.0 40.0 29.0 41.0 56-57 35.879134215454116 38.0 35.0 40.0 28.5 41.0 58-59 35.61466896086192 38.0 34.5 40.0 28.0 41.0 60-61 35.35072409973286 37.5 34.0 40.0 28.0 41.0 62-63 35.047586198755084 37.0 34.0 40.0 27.5 41.0 64-65 34.6764471544212 36.5 34.0 39.0 27.0 41.0 66-67 34.3687891456258 36.0 33.5 39.0 26.0 40.5 68-69 34.03957728723475 35.5 33.0 39.0 26.0 40.0 70-71 33.70939382773486 35.0 33.0 38.0 26.0 40.0 72-73 33.36360727304536 35.0 32.5 37.5 26.0 40.0 74-75 33.02283489999698 35.0 32.0 37.0 25.0 39.0 76-77 32.022113916044994 34.0 30.5 36.0 24.5 38.5 78-79 32.505695008789985 35.0 32.0 36.0 25.0 38.0 80-81 32.4328568575434 35.0 32.0 36.0 25.0 37.5 82-83 32.18028662861673 35.0 32.0 35.0 25.0 37.0 84-85 31.926429253195707 34.0 32.0 35.0 24.5 36.5 86-87 31.687640645574312 34.0 31.0 35.0 24.0 36.0 88-89 31.47925625343026 34.0 31.0 35.0 24.0 36.0 90-91 31.26527438998429 34.0 31.0 35.0 23.0 35.5 92-93 31.087393299019748 34.0 31.0 35.0 23.0 35.0 94-95 30.902240439971482 34.0 30.5 35.0 20.5 35.0 96-97 30.73344823662285 34.0 30.0 35.0 20.0 35.0 98-99 30.562299554656214 34.0 30.0 35.0 20.0 35.0 100 28.819217168765398 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.29294035462191914 1101 2 -0.3054127464911254 1101 3 -0.35431398149675175 1101 4 -0.12559084758903083 1101 5 -0.16944301638384474 1101 6 -0.2543642786397413 1101 7 -0.2830076661104144 1101 8 -0.31212082783481776 1101 9 -0.41830587890409276 1101 10-11 -0.39592797854725603 1101 12-13 -0.42403516861774193 1101 14-15 -0.6212190941628322 1101 16-17 -0.5674727088245319 1101 18-19 -0.6141243989427565 1101 20-21 -0.6212466268360473 1101 22-23 -0.63270428631931 1101 24-25 -0.6286035673631787 1101 26-27 -0.7329376154125171 1101 28-29 -0.7502420443648035 1101 30-31 -0.5907249241857926 1101 32-33 -0.4885366368952688 1101 34-35 -0.564819973825287 1101 36-37 -0.5629492044458217 1101 38-39 -0.5529670151300863 1101 40-41 -0.6457765578955659 1101 42-43 -0.6267190871317894 1101 44-45 -0.6056200900693582 1101 46-47 -0.5952887454042539 1101 48-49 -0.5380828506708184 1101 50-51 -0.5963460675554941 1101 52-53 -0.6676800136339338 1101 54-55 -0.5109890442681078 1101 56-57 -0.6471306636384568 1101 58-59 -0.6367858331183953 1101 60-61 -0.6509557640277919 1101 62-63 -0.747163095120662 1101 64-65 -0.7193703485990426 1101 66-67 -0.6970986074771872 1101 68-69 -0.7478050393129152 1101 70-71 -0.7866758351067347 1101 72-73 -0.7274284682366456 1101 74-75 -0.8003086631274243 1101 76-77 -0.8082819568658621 1101 78-79 -0.602254313697415 1101 80-81 -0.5647980928893048 1101 82-83 -0.565969041868307 1101 84-85 -0.48300746206721357 1101 86-87 -0.6588336246210815 1101 88-89 -0.7252071972128356 1101 90-91 -0.7884350926265817 1101 92-93 -0.832416334183069 1101 94-95 -0.7975883237055648 1101 96-97 -0.8111532501986183 1101 98-99 -0.8490620484858766 1101 100 -0.6675642105601369 1102 1 -0.4211446800881866 1102 2 -0.3339366316730725 1102 3 -0.40027545600479186 1102 4 -0.30521661757939 1102 5 -0.38828671142906757 1102 6 -0.37938927421611623 1102 7 -0.35808156323959395 1102 8 -0.4231255878519633 1102 9 -0.521408583828304 1102 10-11 -0.5379148993577374 1102 12-13 -0.5959204819306692 1102 14-15 -0.7525374502542164 1102 16-17 -0.6910120885781978 1102 18-19 -0.6818711967849822 1102 20-21 -0.7232987885946827 1102 22-23 -0.703372132823219 1102 24-25 -0.7079270577723165 1102 26-27 -0.8206289265281441 1102 28-29 -0.840405212725031 1102 30-31 -0.7441446643300225 1102 32-33 -0.7237913186070131 1102 34-35 -0.7167842539764706 1102 36-37 -0.8049700716660979 1102 38-39 -0.843121650848424 1102 40-41 -0.8082744304900231 1102 42-43 -0.7939604026748697 1102 44-45 -0.8550668111638444 1102 46-47 -0.8532760885498121 1102 48-49 -0.8816941041205126 1102 50-51 -0.984406169888878 1102 52-53 -0.8694103137051528 1102 54-55 -0.8673496146844215 1102 56-57 -1.0121395116504672 1102 58-59 -0.9030216781023981 1102 60-61 -0.9573846492918463 1102 62-63 -1.0574166296831535 1102 64-65 -1.0556410745933533 1102 66-67 -1.0685162982527103 1102 68-69 -1.0401344244578041 1102 70-71 -1.0717312197792594 1102 72-73 -1.2113684555266957 1102 74-75 -1.259356969697265 1102 76-77 -1.0840855636872497 1102 78-79 -0.7950798965221253 1102 80-81 -0.922690625492514 1102 82-83 -0.8088634306280547 1102 84-85 -0.9200770029450922 1102 86-87 -0.9292269840158021 1102 88-89 -1.0264613267582874 1102 90-91 -0.9894897639947935 1102 92-93 -1.0547097640641674 1102 94-95 -1.098931002430266 1102 96-97 -1.2279594547183699 1102 98-99 -1.1304780158518994 1102 100 -1.09813543870656 1103 1 -0.37939844832639125 1103 2 -0.40368933570974264 1103 3 -0.4408104158192714 1103 4 -0.2869089980376103 1103 5 -0.3407297275256269 1103 6 -0.3563790781183087 1103 7 -0.37396119728133925 1103 8 -0.3344350675403618 1103 9 -0.5716971065706815 1103 10-11 -0.5674974517471654 1103 12-13 -0.5676639164131458 1103 14-15 -0.7535181893242466 1103 16-17 -0.7152662062557127 1103 18-19 -0.6806980896106509 1103 20-21 -0.7380896603457501 1103 22-23 -0.7748712980515364 1103 24-25 -0.9465718980126709 1103 26-27 -0.8886867910892278 1103 28-29 -0.8705692815398649 1103 30-31 -0.8441420317695787 1103 32-33 -0.6807621180700281 1103 34-35 -0.8259339604827574 1103 36-37 -0.7753706061788179 1103 38-39 -0.7735357981988642 1103 40-41 -0.7339839422986003 1103 42-43 -0.719714256213912 1103 44-45 -0.8664225592051622 1103 46-47 -0.9277210742133875 1103 48-49 -0.9122201867646709 1103 50-51 -0.9211198579089981 1103 52-53 -0.9234071434552718 1103 54-55 -0.9345378518748859 1103 56-57 -0.8446902832995349 1103 58-59 -0.8587348784370121 1103 60-61 -0.9646778712281616 1103 62-63 -0.8719282588350978 1103 64-65 -0.9526538391289066 1103 66-67 -0.9516851039115082 1103 68-69 -0.9771442387608147 1103 70-71 -1.1014810647670359 1103 72-73 -1.0381935552698494 1103 74-75 -0.9213643720454527 1103 76-77 -0.9616492216461694 1103 78-79 -0.8626494137664267 1103 80-81 -0.7500599593105939 1103 82-83 -0.8274137163564603 1103 84-85 -0.8652059131062444 1103 86-87 -0.8232909131725741 1103 88-89 -0.8789003572465752 1103 90-91 -0.7572402072650277 1103 92-93 -0.7839250375033693 1103 94-95 -0.9952677577980431 1103 96-97 -1.0403630500605914 1103 98-99 -1.100811339184446 1103 100 -1.1956487132823241 1104 1 -0.3630151475631642 1104 2 -0.3162914373451571 1104 3 -0.4261485078420364 1104 4 -0.33989139588834405 1104 5 -0.2761298635222076 1104 6 -0.4036579854298523 1104 7 -0.32725449974749665 1104 8 -0.3647520634211716 1104 9 -0.546565575727918 1104 10-11 -0.581971128906531 1104 12-13 -0.5733801343030152 1104 14-15 -0.7807655119660026 1104 16-17 -0.6678589834321045 1104 18-19 -0.7667286537106506 1104 20-21 -0.858409419029222 1104 22-23 -0.7976126175518061 1104 24-25 -0.7674870585350746 1104 26-27 -0.8111016056658684 1104 28-29 -0.8918978651152472 1104 30-31 -0.8779664919810415 1104 32-33 -0.7738397641383301 1104 34-35 -0.7609184107862461 1104 36-37 -0.7908032259469095 1104 38-39 -0.8058295525976149 1104 40-41 -0.8994839351534267 1104 42-43 -0.8217751045610555 1104 44-45 -0.9353629707913029 1104 46-47 -1.012441167767605 1104 48-49 -1.0518079635064055 1104 50-51 -0.9827129411372368 1104 52-53 -0.9331798880496436 1104 54-55 -1.0003282813835241 1104 56-57 -0.844016701290137 1104 58-59 -0.9113048654685016 1104 60-61 -0.8993075187207396 1104 62-63 -1.0172541355783764 1104 64-65 -0.9986577785044375 1104 66-67 -0.9967271979492196 1104 68-69 -1.004426096435985 1104 70-71 -1.0652471161146764 1104 72-73 -1.066635760296009 1104 74-75 -1.1102879001682808 1104 76-77 -0.8458747645904836 1104 78-79 -0.703944039877058 1104 80-81 -0.6563676045333864 1104 82-83 -0.7983006831921102 1104 84-85 -0.8112994053959994 1104 86-87 -0.8783685604317419 1104 88-89 -0.819862494345891 1104 90-91 -0.8584489185485786 1104 92-93 -0.9349367651243696 1104 94-95 -0.968438725862665 1104 96-97 -0.8146017312033926 1104 98-99 -0.9055818925904902 1104 100 -1.1203525158276726 1105 1 -0.41798605829579216 1105 2 -0.3879414917857389 1105 3 -0.4457687842354012 1105 4 -0.2942815825221814 1105 5 -0.28533442671447773 1105 6 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1105 68-69 -0.9012789930772911 1105 70-71 -0.9220992669340262 1105 72-73 -0.8792760717371166 1105 74-75 -1.016770301644243 1105 76-77 -0.8613949363476827 1105 78-79 -0.8130364939423096 1105 80-81 -0.7572081871529939 1105 82-83 -0.7411931988982197 1105 84-85 -0.7736432820871642 1105 86-87 -0.8121005842432112 1105 88-89 -0.8341297294291188 1105 90-91 -0.8353992613982157 1105 92-93 -0.9105301836730213 1105 94-95 -0.8844067740244874 1105 96-97 -0.9090377519867694 1105 98-99 -0.9790430710098263 1105 100 -1.045576641959432 1106 1 -0.38765186543397334 1106 2 -0.38821366658824275 1106 3 -0.41250644220279753 1106 4 -0.37300960757572454 1106 5 -0.335506498344067 1106 6 -0.31715756493846214 1106 7 -0.41688470114234377 1106 8 -0.436445560175855 1106 9 -0.5266926527120646 1106 10-11 -0.5778873058870246 1106 12-13 -0.5954789338114992 1106 14-15 -0.7600127791521629 1106 16-17 -0.7555991359115595 1106 18-19 -0.704676495099477 1106 20-21 -0.7640685782517451 1106 22-23 -0.6812883177498037 1106 24-25 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0.7882904918232612 2311 32-33 0.8885782229964008 2311 34-35 0.977114810824304 2311 36-37 1.0062233482822833 2311 38-39 0.9715448466767285 2311 40-41 0.9701624867599392 2311 42-43 0.9313592669720947 2311 44-45 0.9705180428983695 2311 46-47 0.6352211344993748 2311 48-49 0.842809766666683 2311 50-51 0.9712179120512516 2311 52-53 0.9082241863696296 2311 54-55 0.9083787491044291 2311 56-57 1.0469044077455507 2311 58-59 1.0077005613789183 2311 60-61 1.0715518756644613 2311 62-63 1.0842302696856123 2311 64-65 1.1458675902986712 2311 66-67 1.2101793377696382 2311 68-69 1.1304298299992297 2311 70-71 1.136454235703134 2311 72-73 1.1524582566086607 2311 74-75 1.1633789144122062 2311 76-77 1.147058507437361 2311 78-79 0.8297640737801331 2311 80-81 0.743715684587805 2311 82-83 0.8266325653262427 2311 84-85 0.8824145811106092 2311 86-87 0.9575019508490037 2311 88-89 1.0266924924861662 2311 90-91 1.0488917363306491 2311 92-93 1.1185854149038583 2311 94-95 1.1444945145177918 2311 96-97 1.1028259984264146 2311 98-99 1.1725316199287974 2311 100 1.1934316283981765 2312 1 0.20862217302756392 2312 2 0.3071334834464068 2312 3 0.4367271004279232 2312 4 0.16669755073022685 2312 5 0.33385791104050355 2312 6 0.3883540959159433 2312 7 0.29243074411060377 2312 8 0.40988202516382444 2312 9 0.6399057966988906 2312 10-11 0.6350543423368862 2312 12-13 0.6441334608412106 2312 14-15 0.811558823467827 2312 16-17 0.8264262808668761 2312 18-19 0.7723534834678034 2312 20-21 0.8570707547071379 2312 22-23 0.9056215663122913 2312 24-25 0.8677695053072298 2312 26-27 0.8829073157199332 2312 28-29 0.9097596338696903 2312 30-31 0.9612070148429126 2312 32-33 0.813920892069973 2312 34-35 0.9156432645622203 2312 36-37 0.9634819043134826 2312 38-39 0.7451200829570155 2312 40-41 0.8259119115488645 2312 42-43 0.9880049660214354 2312 44-45 0.6931549699960229 2312 46-47 0.7718222538245598 2312 48-49 0.8759935409651831 2312 50-51 0.7737973415780957 2312 52-53 0.9527417027204663 2312 54-55 0.7910944210692108 2312 56-57 0.8502597388010216 2312 58-59 1.0229771819040892 2312 60-61 1.0020498454995561 2312 62-63 1.050964474675915 2312 64-65 1.0902258419228161 2312 66-67 1.0760951355622765 2312 68-69 1.1350747996678976 2312 70-71 0.9824423097477393 2312 72-73 1.0266479005662248 2312 74-75 1.1032295575216011 2312 76-77 1.0398256624899993 2312 78-79 0.9143036958479698 2312 80-81 0.8985118193687143 2312 82-83 0.9095261657447153 2312 84-85 0.9235567181295039 2312 86-87 0.9584541885994149 2312 88-89 1.0274950742832694 2312 90-91 1.0575769598370819 2312 92-93 1.0868660093629465 2312 94-95 1.1080258273503816 2312 96-97 0.759845373534926 2312 98-99 0.5764776551744433 2312 100 0.7512221975670847 2313 1 -0.025513665688428944 2313 2 0.15198570501618747 2313 3 0.2601846768742959 2313 4 0.17744739761648987 2313 5 0.3433380188830881 2313 6 0.23157210461658906 2313 7 0.20028453154454695 2313 8 0.3351345838995172 2313 9 0.5710885134732067 2313 10-11 0.3609300897528911 2313 12-13 0.4219652756651513 2313 14-15 0.4627280914894669 2313 16-17 0.711421647992573 2313 18-19 0.6833698587448254 2313 20-21 0.6556640420116437 2313 22-23 0.6034923777835104 2313 24-25 0.4972890044201179 2313 26-27 0.7458694982062468 2313 28-29 0.5745542089080473 2313 30-31 0.8218409609468864 2313 32-33 0.8700165550050798 2313 34-35 0.8604931973184549 2313 36-37 0.9392327535422353 2313 38-39 0.9051403902578627 2313 40-41 0.9317865855902454 2313 42-43 1.046027494260663 2313 44-45 0.8066542344187013 2313 46-47 0.852868391570091 2313 48-49 0.9779422289225437 2313 50-51 0.9987342018941732 2313 52-53 1.0601994070246903 2313 54-55 1.0226397569407695 2313 56-57 0.7215739202897353 2313 58-59 0.7898095902410489 2313 60-61 0.721068978402279 2313 62-63 1.0225650735558176 2313 64-65 1.0784190367276807 2313 66-67 1.0618289212914434 2313 68-69 1.1660448540943094 2313 70-71 0.9896697120548552 2313 72-73 1.0595596186601668 2313 74-75 1.0864084748654363 2313 76-77 1.0391055543948688 2313 78-79 0.9143824231413689 2313 80-81 0.9031982834832704 2313 82-83 0.9270985317149467 2313 84-85 0.9129256922359161 2313 86-87 0.9120889823536338 2313 88-89 0.9432480702612729 2313 90-91 0.8550241040291802 2313 92-93 1.0183888517220971 2313 94-95 1.0389596948454418 2313 96-97 1.1730126416070839 2313 98-99 1.22720111756529 2313 100 1.270831489202969 2314 1 -0.42051112601977536 2314 2 -0.015713233669650606 2314 3 0.2313140213481475 2314 4 0.2076749227640704 2314 5 0.3056433818369726 2314 6 0.36587409673754223 2314 7 0.42427753469664964 2314 8 0.4474890997706211 2314 9 0.40375366484474995 2314 10-11 0.5544069690469442 2314 12-13 0.47588675494315424 2314 14-15 0.8109046640790325 2314 16-17 0.7473903888029767 2314 18-19 0.8153996676499489 2314 20-21 0.8341929510963695 2314 22-23 0.8934364898837615 2314 24-25 0.7779932490691976 2314 26-27 0.6791784373538405 2314 28-29 0.7269495174474159 2314 30-31 0.8185510115944368 2314 32-33 0.8605751241387551 2314 34-35 0.92660068119946 2314 36-37 0.8826182685603001 2314 38-39 0.5905306577372187 2314 40-41 0.6475037928782399 2314 42-43 0.8208475909381932 2314 44-45 0.9311630090346554 2314 46-47 0.9238642059774307 2314 48-49 0.948309141171535 2314 50-51 0.9278546623883486 2314 52-53 0.9073618589350403 2314 54-55 0.8422519683724303 2314 56-57 0.8320498863967032 2314 58-59 0.9628898316189307 2314 60-61 0.9887150427664722 2314 62-63 0.8354123793197701 2314 64-65 0.8870683704595876 2314 66-67 0.5836283767816255 2314 68-69 0.6559505066190283 2314 70-71 0.8409931497940946 2314 72-73 0.9031369910630858 2314 74-75 0.9152136184843087 2314 76-77 0.832377741256149 2314 78-79 0.8047875944657079 2314 80-81 0.861171172216622 2314 82-83 0.8195102235474181 2314 84-85 0.9273280589883797 2314 86-87 0.8931529577006181 2314 88-89 0.9148208694378788 2314 90-91 1.033493774431225 2314 92-93 0.9579003087871136 2314 94-95 0.9610425998375298 2314 96-97 0.9971610171912708 2314 98-99 1.1256504463150705 2314 100 1.1257823848918527 2315 1 0.31760426746536297 2315 2 0.31930991715348966 2315 3 0.4094292672244748 2315 4 0.28804088947181583 2315 5 0.3255539895414046 2315 6 0.3708001361335249 2315 7 0.34490079972451326 2315 8 0.23838136364380347 2315 9 0.5150912442565172 2315 10-11 0.5421988111128044 2315 12-13 0.3742425243090466 2315 14-15 0.5558409238680966 2315 16-17 0.7175199782108947 2315 18-19 0.7398927603162306 2315 20-21 0.4840437903787915 2315 22-23 0.7993405538675233 2315 24-25 0.8314733844123126 2315 26-27 0.7325460209845858 2315 28-29 0.8374293734459215 2315 30-31 0.7949455078485599 2315 32-33 0.7444505204709202 2315 34-35 0.820510341983308 2315 36-37 0.8054474763792356 2315 38-39 0.8942052626233377 2315 40-41 0.910579986621471 2315 42-43 1.0018547814078431 2315 44-45 0.9825925952376409 2315 46-47 1.0096653360744696 2315 48-49 0.9130949380502926 2315 50-51 0.9583947468226128 2315 52-53 0.9932536774542555 2315 54-55 0.7586660318921403 2315 56-57 0.6872746668008034 2315 58-59 0.8092628356269174 2315 60-61 0.9779355560629384 2315 62-63 0.9533084908287108 2315 64-65 1.0114043456871897 2315 66-67 1.0061381783600112 2315 68-69 1.0402940048404972 2315 70-71 0.9684159182902121 2315 72-73 1.0539882591187464 2315 74-75 0.42832815513067146 2315 76-77 0.404344286833048 2315 78-79 0.7240242972126154 2315 80-81 0.7700645672180855 2315 82-83 0.727000551379497 2315 84-85 0.781382433016347 2315 86-87 0.7401974048343725 2315 88-89 0.7348961266740268 2315 90-91 0.7937754352809954 2315 92-93 0.8292782945922035 2315 94-95 0.9360897866311113 2315 96-97 1.0255054712302858 2315 98-99 1.0205856091483057 2315 100 1.107181249254424 2316 1 0.26230814688521065 2316 2 0.1044165373818231 2316 3 0.23795970930278543 2316 4 0.029864397061963643 2316 5 0.3000060931100492 2316 6 0.34390042169624024 2316 7 0.4134363320689829 2316 8 0.44602227514873505 2316 9 0.6639352404708205 2316 10-11 0.6713400598907739 2316 12-13 0.4238541063101664 2316 14-15 0.5169527593477099 2316 16-17 0.8457440427016039 2316 18-19 0.8057739869007605 2316 20-21 0.729299504825967 2316 22-23 0.7913457467062841 2316 24-25 0.5265860903456101 2316 26-27 0.7491244055560173 2316 28-29 0.88556618409131 2316 30-31 0.5634295368356135 2316 32-33 0.7196677288011699 2316 34-35 0.8163199964171284 2316 36-37 0.9429406056052159 2316 38-39 0.8199693792655367 2316 40-41 0.8099350916955359 2316 42-43 0.8149100369940783 2316 44-45 0.6145016257605107 2316 46-47 0.7948096007493248 2316 48-49 0.9259755350147358 2316 50-51 0.9331854232560843 2316 52-53 0.9582383099891842 2316 54-55 0.8370330241308963 2316 56-57 0.9653340522970453 2316 58-59 0.8623902549735405 2316 60-61 0.9125446916167235 2316 62-63 0.9039983294126657 2316 64-65 0.9151751077415327 2316 66-67 0.9037503821570212 2316 68-69 0.9337616471987218 2316 70-71 0.9194492514064834 2316 72-73 0.9569643990261554 2316 74-75 0.9078952089129899 2316 76-77 0.8149888894876476 2316 78-79 0.47669165170125183 2316 80-81 0.5059255377335532 2316 82-83 0.43765883626974045 2316 84-85 0.5719246059269949 2316 86-87 0.6055364554795482 2316 88-89 0.6390897230646821 2316 90-91 0.517069369530244 2316 92-93 0.38279745343228555 2316 94-95 0.4501006256068223 2316 96-97 0.6737438373295568 2316 98-99 0.7806855649340605 2316 100 0.46000637978335845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 3.0 11 4.0 12 27.0 13 100.0 14 507.0 15 1401.0 16 3433.0 17 7265.0 18 12345.0 19 17682.0 20 24301.0 21 33409.0 22 40839.0 23 42899.0 24 42308.0 25 44317.0 26 51108.0 27 62264.0 28 76586.0 29 94596.0 30 116522.0 31 145036.0 32 184656.0 33 242935.0 34 348244.0 35 486854.0 36 750736.0 37 1158587.0 38 1016160.0 39 162095.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36574303627655 19.730990236818194 13.37738094569679 23.525885781208462 2 15.786906908208708 20.127847649540065 40.75669738065398 23.328548061597246 3 19.094201860798524 26.041077216403945 29.53489326224966 25.329827660547867 4 12.58757152711269 16.5483106756194 38.036616693276684 32.82750110399123 5 13.030369767713697 37.640684279666296 34.15652521924647 15.172420733373535 6 30.38372099324127 36.54632914567224 18.557629560361384 14.512320300725106 7 27.594882324822585 32.302682889539575 22.10692852401402 17.995506261623824 8 26.40566228341452 35.391109845016025 19.92079336683153 18.28243450473792 9 26.93857414155772 15.95832065595651 20.912445971148998 36.190659231336774 10-11 25.669273707770973 26.113099982398673 27.553679930105613 20.66394637972474 12-13 25.67256852445298 23.861573763804024 27.97256680206447 22.493290909678525 14-15 23.054310181914488 25.213095087305387 26.16459033074842 25.5680044000317 16-17 22.041217243937542 27.738969647280104 27.16648696047138 23.053326148310976 18-19 21.56054562161183 27.162942179622252 29.44148757055018 21.835024628215738 20-21 22.776790522217606 26.495272865556434 29.074056423711486 21.653880188514474 22-23 22.692144980960837 26.301293300645373 28.902244600796866 22.104317117596924 24-25 22.177572109222893 26.158112104328573 29.505515883947897 22.158799902500643 26-27 22.061434019095575 26.462476862551874 28.97370219587192 22.502386922480635 28-29 21.826535678507394 27.0175802902732 29.103686100623825 22.052197930595575 30-31 22.703846111045795 26.484814264861207 29.13758726481621 21.67375235927679 32-33 22.696831207652973 26.575325655547555 28.684418267931242 22.04342486886823 34-35 22.609720344933887 26.728136870168758 28.975491768887135 21.686651016010224 36-37 22.40459457929081 26.646614343492907 28.749301274137196 22.19948980307909 38-39 22.404757083962274 26.79668206575707 28.922296600185902 21.876264250094753 40-41 22.394791498941004 26.1071732136443 29.315816710430166 22.182218576984532 42-43 22.25688181698547 26.427241622219988 28.707724046105852 22.608152514688694 44-45 22.02655181216931 26.974977804779996 28.794714876342574 22.203755506708113 46-47 22.063188770871143 26.611480313767256 28.29900856771468 23.026322347646925 48-49 22.521677166163514 26.598366231713776 28.327014152886942 22.552942449235765 50-51 22.400727587049833 26.80517246418242 28.15782398981781 22.636275958949938 52-53 22.09069324936434 27.132790972287946 27.93531964211371 22.841196136234004 54-55 22.050593670425116 26.964655578444596 28.135738511531557 22.849012239598732 56-57 22.613213681631517 26.76543673387237 27.6873347757595 22.934014808736613 58-59 21.996661753229862 27.039276269338913 28.235658777297445 22.72840320013378 60-61 22.426758046860606 27.317173809711814 27.80966614179568 22.4464020016319 62-63 22.541517596699087 26.987833684411594 27.638537216601755 22.832111502287564 64-65 22.0143578064329 26.90102417754398 27.760577905387233 23.324040110635877 66-67 22.167883360796452 26.709207634059766 27.72841710093057 23.39449190421321 68-69 21.83116360038186 26.82613864990521 27.967896472465405 23.374801277247528 70-71 21.91601518688275 26.593193242791752 28.034053439107492 23.456738131218007 72-73 22.161606695986453 26.912922552970684 27.7507727664798 23.174697984563064 74-75 22.343906783633045 27.06784755909512 27.396575920473293 23.191669736798545 76-77 22.112324434993905 26.87684586209407 27.41017127122306 23.60065843168897 78-79 21.890767013204247 26.95183126959883 27.538879263741027 23.61852245345589 80-81 21.739086259738247 27.26720642484538 27.542162418823153 23.451544896593226 82-83 22.3216389383375 26.806145631566057 28.027518273399682 22.84469715669676 84-85 22.45224989524834 27.093455131824445 27.237900118421653 23.21639485450556 86-87 21.67088667401973 27.429424927502104 27.54582044998265 23.353867948495513 88-89 21.978350136428826 26.757934237423438 27.589066056843357 23.674649569304382 90-91 22.20628509796378 26.667157817512805 27.651282770160474 23.475274314362938 92-93 22.183615459346477 26.89689199962983 27.51625982104033 23.403232719983365 94-95 21.501232778624082 26.906868091961407 27.70875243306477 23.88314669634974 96-97 21.546333303367845 26.886144342749947 27.589707522978994 23.97781483090321 98-99 21.287244518968567 27.17683751563676 27.379194703948084 24.15672326144659 100 21.46644039867151 27.026771174293724 27.225994646074884 24.28079378095988 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2136.0 1 1531.0 2 2087.5 3 3072.5 4 2049.0 5 1362.0 6 1962.5 7 3415.5 8 5154.5 9 5835.0 10 5034.5 11 4054.5 12 3980.0 13 4339.0 14 4927.0 15 5692.5 16 6363.5 17 6953.5 18 7674.5 19 8531.0 20 9285.0 21 10012.0 22 11283.5 23 13191.0 24 15141.0 25 17657.5 26 21165.5 27 25476.0 28 30867.5 29 38808.0 30 49496.5 31 60302.0 32 73789.5 33 88466.5 34 101233.0 35 113940.5 36 125986.0 37 141817.5 38 150692.0 39 150376.5 40 148364.0 41 144889.0 42 146143.0 43 152624.5 44 162968.0 45 180038.0 46 195040.5 47 205455.0 48 216369.5 49 220635.0 50 218840.0 51 219553.5 52 217195.0 53 207573.5 54 196335.0 55 177157.0 56 156955.5 57 135578.5 58 114603.0 59 101141.0 60 82079.5 61 61072.5 62 46686.0 63 36701.0 64 26849.5 65 17262.0 66 11598.5 67 8400.0 68 6382.5 69 4860.5 70 3706.5 71 2747.5 72 2077.0 73 1667.5 74 1287.0 75 910.0 76 598.0 77 340.0 78 187.0 79 106.5 80 68.0 81 40.5 82 27.0 83 20.5 84 9.0 85 6.0 86 5.5 87 2.5 88 2.5 89 3.5 90 3.0 91 1.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043098409906712355 2 0.0 3 0.0 4 0.03667332140692408 5 0.0 6 0.0 7 0.0010450445150258047 8 0.0 9 0.010837498674341679 10-11 3.7737718598154064E-4 12-13 1.935267620418157E-5 14-15 1.6449774773554334E-4 16-17 2.612611287564512E-4 18-19 0.0 20-21 2.9029014306272356E-5 22-23 0.0 24-25 5.902566242275379E-4 26-27 4.934932432066301E-4 28-29 1.6449774773554334E-4 30-31 0.0027384036828916923 32-33 0.015104763777363717 34-35 0.025671324984846856 36-37 0.03079978417895497 38-39 0.02202334552035863 40-41 0.0014708033915177994 42-43 7.741070481672628E-5 44-45 0.011437431636671309 46-47 0.018007665207990953 48-49 0.003309307630915049 50-51 0.011166494169812767 52-53 0.011127788817404404 54-55 0.010266594726318324 56-57 4.644642289003577E-4 58-59 0.008902231053923522 60-61 0.005031695813087209 62-63 0.001306305643782256 64-65 0.03543475012985646 66-67 0.05893857537983498 68-69 0.05832896607940325 70-71 0.05139103166020416 72-73 0.04565296316566433 74-75 0.03647979464488226 76-77 0.03166097827004105 78-79 0.015501493639549438 80-81 0.001035368176923714 82-83 0.03771836592194988 84-85 0.0512458865886728 86-87 0.05720651085956073 88-89 0.06894390897739684 90-91 0.048449424877168565 92-93 0.039827807628205675 94-95 0.012840500661474472 96-97 0.003647979464488226 98-99 0.021897553125031447 100 0.032667317432658494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5167244.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.83988899196079 #Duplication Level Percentage of deduplicated Percentage of total 1 80.22338795004778 55.225691210402935 2 13.241253173003232 18.230527970879816 3 3.436498144216629 7.097044523068561 4 1.2621499428450667 3.475450478266561 5 0.5851052604763524 2.01392905899022 6 0.3271873769329987 1.3514125624583075 7 0.1922988968182705 0.9266484297172384 8 0.1407082546024201 0.7749072501666523 9 0.10232283117695175 0.6339503105608052 >10 0.4500348089336916 5.408476232154145 >50 0.024466529342165044 1.1676699269247655 >100 0.013294586790016497 1.7379512894724307 >500 8.399594697068788E-4 0.38013980479855536 >1k 3.553670565394892E-4 0.5635262003875309 >5k 0.0 0.0 >10k+ 9.691828814699626E-5 1.0126747517514192 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19225 0.3720552000253907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16243 0.3143455195845213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10067 0.19482339134749588 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.935267620418157E-5 0.0 0.0 0.0 6 1.935267620418157E-5 1.935267620418157E-5 0.0 0.0 0.0 7 1.935267620418157E-5 1.935267620418157E-5 0.0 0.0 0.0 8 1.935267620418157E-5 1.935267620418157E-5 0.0 0.0 5.805802861254471E-5 9 3.870535240836314E-5 1.935267620418157E-5 0.0 0.0 5.805802861254471E-5 10-11 3.870535240836314E-5 1.935267620418157E-5 0.0 0.0 5.805802861254471E-5 12-13 3.870535240836314E-5 1.935267620418157E-5 0.0 2.9029014306272356E-5 5.805802861254471E-5 14-15 3.870535240836314E-5 1.935267620418157E-5 0.0 6.77343667146355E-5 7.741070481672628E-5 16-17 3.870535240836314E-5 1.935267620418157E-5 0.0 7.741070481672628E-5 7.741070481672628E-5 18-19 3.870535240836314E-5 1.935267620418157E-5 0.0 1.5482140963345257E-4 9.676338102090785E-5 20-21 3.870535240836314E-5 1.935267620418157E-5 0.0 2.3223211445017885E-4 9.676338102090785E-5 22-23 3.870535240836314E-5 1.935267620418157E-5 0.0 4.3543521459408536E-4 1.1611605722508942E-4 24-25 3.870535240836314E-5 1.935267620418157E-5 0.0 0.0011127788817404405 1.35468733429271E-4 26-27 3.870535240836314E-5 1.935267620418157E-5 0.0 0.002302968468297607 1.451450715313618E-4 28-29 3.870535240836314E-5 1.935267620418157E-5 0.0 0.005863860889867015 1.5482140963345257E-4 30-31 3.870535240836314E-5 1.935267620418157E-5 0.0 0.01971070071395893 1.7417408583763415E-4 32-33 3.870535240836314E-5 2.9029014306272356E-5 0.0 0.052445752513332056 1.7417408583763415E-4 34-35 3.870535240836314E-5 3.870535240836314E-5 0.0 0.09995657259459781 1.7417408583763415E-4 36-37 3.870535240836314E-5 4.838169051045393E-5 0.0 0.1759835610627251 1.7417408583763415E-4 38-39 3.870535240836314E-5 5.805802861254471E-5 0.0 0.28978697348141486 1.7417408583763415E-4 40-41 3.870535240836314E-5 5.805802861254471E-5 0.0 0.41803715868652613 1.7417408583763415E-4 42-43 3.870535240836314E-5 7.741070481672628E-5 0.0 0.5362820102940755 2.128794382459973E-4 44-45 3.870535240836314E-5 7.741070481672628E-5 0.0 0.6751761674114867 2.3223211445017885E-4 46-47 5.805802861254471E-5 7.741070481672628E-5 0.0 0.8344970742624115 2.3223211445017885E-4 48-49 5.805802861254471E-5 7.741070481672628E-5 0.0 1.0090969189765375 2.902901430627236E-4 50-51 5.805802861254471E-5 8.708704291881706E-5 0.0 1.1931989277069168 2.902901430627236E-4 52-53 5.805802861254471E-5 9.676338102090785E-5 0.0 1.3719015397763294 3.386718335731775E-4 54-55 6.77343667146355E-5 9.676338102090785E-5 0.0 1.5717759796131168 3.6770084787944986E-4 56-57 7.741070481672628E-5 9.676338102090785E-5 0.0 1.7896871136722012 3.6770084787944986E-4 58-59 7.741070481672628E-5 1.1611605722508942E-4 0.0 2.046690653663733 3.6770084787944986E-4 60-61 7.741070481672628E-5 1.1611605722508942E-4 0.0 2.306984148609975 3.967298621857222E-4 62-63 7.741070481672628E-5 1.1611605722508942E-4 0.0 2.56937740892437 4.06406200287813E-4 64-65 8.708704291881706E-5 1.1611605722508942E-4 0.0 2.8277162835739906 4.06406200287813E-4 66-67 9.676338102090785E-5 1.1611605722508942E-4 0.0 3.1197578438331925 4.06406200287813E-4 68-69 9.676338102090785E-5 1.1611605722508942E-4 0.0 3.4583909720539614 4.06406200287813E-4 70-71 9.676338102090785E-5 1.1611605722508942E-4 0.0 3.8084518555733 4.06406200287813E-4 72-73 9.676338102090785E-5 1.1611605722508942E-4 0.0 4.142653220943311 4.06406200287813E-4 74-75 9.676338102090785E-5 1.1611605722508942E-4 0.0 4.471987388247971 4.06406200287813E-4 76-77 1.35468733429271E-4 1.1611605722508942E-4 0.0 4.839581796408298 4.06406200287813E-4 78-79 1.35468733429271E-4 1.1611605722508942E-4 0.0 5.240366044258796 4.06406200287813E-4 80-81 1.35468733429271E-4 1.1611605722508942E-4 0.0 5.654842310523753 4.160825383899038E-4 82-83 1.35468733429271E-4 1.1611605722508942E-4 0.0 6.077340261075343 4.644642289003577E-4 84-85 1.35468733429271E-4 1.1611605722508942E-4 0.0 6.499751124584014 4.644642289003577E-4 86-87 1.5482140963345257E-4 1.1611605722508942E-4 0.0 6.941940423173359 4.644642289003577E-4 88 1.5482140963345257E-4 1.1611605722508942E-4 0.0 7.29446877290873 4.644642289003577E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9560 0.0 52.133793 1 TACCTGG 4870 0.0 48.638893 2 GTATCAA 16825 0.0 39.459442 1 ACCTGGG 5925 0.0 38.792225 3 GTACCTG 6215 0.0 38.50774 1 ATCAACG 19920 0.0 32.515022 3 CAACGCA 20035 0.0 32.387165 5 CCTGGGG 5295 0.0 31.779074 4 GTGGTAT 3475 0.0 31.52626 1 TATCAAC 21190 0.0 31.073439 2 AACGCAG 21950 0.0 30.011114 6 TCAACGC 21820 0.0 29.748365 4 GTACTAG 3035 0.0 28.350695 1 GTACATG 20485 0.0 27.887632 1 CTGGGGG 4190 0.0 27.13751 5 TACATGG 20675 0.0 27.051016 2 ACATGGG 20475 0.0 26.583294 3 ACGCAGA 24545 0.0 26.359644 7 CGCAGAG 25005 0.0 25.855682 8 TGGGGGG 11995 0.0 25.304682 6 >>END_MODULE