FastQCFastQC Report
Mon 6 Jun 2016
ERR1211225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10745576
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363180.3379809514166574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281850.26229398963815437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176310.1640768256629519No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220450.043.379661
GTATCAA383700.039.335051
GTACTAG107400.037.7724841
GCATCGT105850.034.6376388
CAACGCA429550.033.4982765
ATCAACG431700.033.277313
GGGCACC107650.032.91766794
TAGGCAT131800.032.135295
TATCAAC476150.031.4699292
CTGTGCG132050.031.0043229
GTGGTAT75050.030.2878881
AACGCAG490350.030.1313866
CTAGGCA147250.029.5932884
TACCTGG57500.029.464642
TAGTACT143150.028.897494
TCAACGC510200.028.4791724
ACTGTGC152600.028.0305358
ACGCAGA521150.027.5657317
CGCAGAG524400.027.2941258
CATCGTC137650.026.9428359
Produced by FastQC (version 0.11.5)