##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211225_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10745576 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44744814051848 31.0 31.0 34.0 29.0 34.0 2 31.465625760778202 31.0 31.0 34.0 28.0 34.0 3 31.53401064773075 33.0 31.0 34.0 28.0 34.0 4 35.13397336727226 37.0 35.0 37.0 32.0 37.0 5 35.195630369186354 37.0 35.0 37.0 32.0 37.0 6 35.25421708431451 37.0 35.0 37.0 32.0 37.0 7 35.16626730851841 37.0 35.0 37.0 32.0 37.0 8 35.177133361673675 37.0 35.0 37.0 32.0 37.0 9 36.7320514042244 39.0 37.0 39.0 32.0 39.0 10-11 36.689360160869924 39.0 37.0 39.0 32.0 39.0 12-13 36.57569254547174 39.0 37.0 39.0 32.0 39.0 14-15 37.75642101456451 40.0 37.0 41.0 32.0 41.0 16-17 37.740843859835906 40.0 37.0 41.0 32.0 41.0 18-19 37.764198261684626 40.0 37.0 41.0 32.0 41.0 20-21 37.73335589455605 40.0 37.0 41.0 32.0 41.0 22-23 37.62126813862747 40.0 37.0 41.0 32.0 41.0 24-25 37.524727059768594 40.0 37.0 41.0 31.5 41.0 26-27 37.37393230479222 40.0 36.5 41.0 31.0 41.0 28-29 37.25554730616581 40.0 36.0 41.0 31.0 41.0 30-31 37.10553133680317 39.5 36.0 41.0 30.5 41.0 32-33 36.92603267614505 39.0 36.0 41.0 30.0 41.0 34-35 36.78203709135741 39.0 35.0 41.0 30.0 41.0 36-37 36.67720455376241 39.0 35.0 41.0 30.0 41.0 38-39 36.53261360768376 39.0 35.0 41.0 30.0 41.0 40-41 36.35964949668589 39.0 35.0 40.0 29.0 41.0 42-43 36.14038521527371 39.0 35.0 40.0 28.5 41.0 44-45 35.89154234263478 38.0 35.0 40.0 27.5 41.0 46-47 35.63458729434327 38.0 34.0 40.0 27.0 41.0 48-49 35.405994708892294 38.0 34.0 40.0 26.0 41.0 50-51 34.702823143217266 37.5 33.5 39.5 25.0 40.5 52-53 34.79464730415569 38.0 33.5 39.5 26.0 40.5 54-55 35.26204076915002 38.0 34.0 40.0 26.0 41.0 56-57 35.20019620167407 38.0 34.0 40.0 26.0 41.0 58-59 34.966452752276844 37.5 34.0 40.0 26.0 41.0 60-61 34.74488082351286 37.0 34.0 40.0 26.0 41.0 62-63 34.44518199861971 36.5 33.0 40.0 26.0 41.0 64-65 34.19724573163877 36.0 33.0 39.5 25.0 41.0 66-67 34.03362793209038 35.5 33.0 39.0 25.0 41.0 68-69 33.751614664490766 35.0 33.0 39.0 25.0 41.0 70-71 33.48526979847334 35.0 33.0 38.5 24.5 41.0 72-73 33.17582426479511 35.0 32.0 37.5 24.0 40.0 74-75 32.860184879805416 35.0 32.0 37.0 23.5 39.5 76-77 32.550011092937225 35.0 32.0 36.5 23.0 39.0 78-79 32.227623861205764 35.0 31.5 36.0 22.5 39.0 80-81 31.94367575083923 35.0 31.0 36.0 21.5 38.5 82-83 31.655167950047534 35.0 31.0 35.0 20.5 37.0 84-85 31.41795358387489 35.0 31.0 35.0 20.0 37.0 86-87 31.19475693997232 34.0 31.0 35.0 20.0 36.5 88-89 30.994601080481864 34.0 30.5 35.0 20.0 36.0 90-91 30.764165597079206 34.0 30.0 35.0 19.0 36.0 92-93 30.562645827454944 34.0 30.0 35.0 18.5 35.5 94-95 30.400721934310454 34.0 29.5 35.0 18.0 35.0 96-97 30.21986745987372 34.0 29.0 35.0 18.0 35.0 98-99 29.984764055458733 34.0 29.0 35.0 17.0 35.0 100 28.82278502334356 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4600102993694257 1101 2 -0.4684088134996749 1101 3 -0.47200857946306485 1101 4 -0.3565774687110732 1101 5 -0.31905072341627516 1101 6 -0.3415055566030034 1101 7 -0.3168521358253358 1101 8 -0.3822514104478074 1101 9 -0.5441007960050257 1101 10-11 -0.5350412626660415 1101 12-13 -0.6189967530251934 1101 14-15 -0.7191339084932835 1101 16-17 -0.7540920069106463 1101 18-19 -0.6805918964567539 1101 20-21 -0.7374070487168396 1101 22-23 -0.7794116976387286 1101 24-25 -0.8705784360825106 1101 26-27 -0.9379677611823993 1101 28-29 -0.8746927508936153 1101 30-31 -0.8655460590996071 1101 32-33 -0.8998747190923098 1101 34-35 -0.8670159241001869 1101 36-37 -0.9761978298589185 1101 38-39 -1.0141653303195284 1101 40-41 -0.9988509086158004 1101 42-43 -1.111781604953265 1101 44-45 -1.0981408093381901 1101 46-47 -1.255544181785119 1101 48-49 -1.2202649612792484 1101 50-51 -1.2898020400998789 1101 52-53 -1.076712183480943 1101 54-55 -0.9252471533515916 1101 56-57 -0.9255826853321807 1101 58-59 -0.920623264344087 1101 60-61 -0.8557126142545428 1101 62-63 -0.9108018995300853 1101 64-65 -0.8876282265107065 1101 66-67 -0.9805457915323714 1101 68-69 -0.9844620315511889 1101 70-71 -0.9684886692269146 1101 72-73 -1.0564675547305598 1101 74-75 -1.0242717172996478 1101 76-77 -1.0295808082692162 1101 78-79 -1.0985145337035362 1101 80-81 -1.2060683061085058 1101 82-83 -1.1968234681903134 1101 84-85 -1.2225647354292626 1101 86-87 -1.2984565662025496 1101 88-89 -1.327759626241047 1101 90-91 -1.3189626502455987 1101 92-93 -1.4588336636357333 1101 94-95 -1.5359996347795608 1101 96-97 -1.5959474267527547 1101 98-99 -1.6855921438025412 1101 100 -1.710639250443112 1102 1 -0.5051836068863906 1102 2 -0.3980459363295594 1102 3 -0.4382948685663415 1102 4 -0.2663714349834976 1102 5 -0.3407013599827522 1102 6 -0.4081471720222183 1102 7 -0.3393106953052438 1102 8 -0.3457050616494186 1102 9 -0.49384792917784637 1102 10-11 -0.524534445917368 1102 12-13 -0.5527788679769401 1102 14-15 -0.7413123145205205 1102 16-17 -0.7017170358390175 1102 18-19 -0.7581470573044413 1102 20-21 -0.7871447605838782 1102 22-23 -0.7942684562927056 1102 24-25 -0.7253817734151653 1102 26-27 -0.7926378468974846 1102 28-29 -0.8696314839371553 1102 30-31 -0.8306678837323602 1102 32-33 -0.9140013451386295 1102 34-35 -0.9493220458116411 1102 36-37 -0.8712494602929581 1102 38-39 -0.9522405873998636 1102 40-41 -1.0996817192791681 1102 42-43 -1.0607450403652265 1102 44-45 -1.19661628824678 1102 46-47 -1.1860035204215578 1102 48-49 -1.1575026473861598 1102 50-51 -1.1537302079175475 1102 52-53 -0.9934378789287237 1102 54-55 -0.8559141771263654 1102 56-57 -0.8542674213317625 1102 58-59 -0.9564984792181619 1102 60-61 -0.8642583249663431 1102 62-63 -1.0059495191435417 1102 64-65 -0.9724754982103931 1102 66-67 -0.9712700066379583 1102 68-69 -0.9595381206255666 1102 70-71 -1.0676216477763916 1102 72-73 -1.0439845901489164 1102 74-75 -1.0620981643762022 1102 76-77 -1.119253325635114 1102 78-79 -1.1472908400362556 1102 80-81 -1.221887899054984 1102 82-83 -1.18960308206103 1102 84-85 -1.1918486388569356 1102 86-87 -1.2656506160164334 1102 88-89 -1.2559582870240504 1102 90-91 -1.3338240085448554 1102 92-93 -1.336126292027501 1102 94-95 -1.293309400621073 1102 96-97 -1.4557709847846745 1102 98-99 -1.4640256796824396 1102 100 -1.5562820170908687 1103 1 -0.5779595329013176 1103 2 -0.5136765718620033 1103 3 -0.5628278656036301 1103 4 -0.48769917764756343 1103 5 -0.4262398907858511 1103 6 -0.5120106227944916 1103 7 -0.5024970725703426 1103 8 -0.46553974645566853 1103 9 -0.6820226539264382 1103 10-11 -0.6376659025188616 1103 12-13 -0.741568999590065 1103 14-15 -0.8453133651039195 1103 16-17 -0.8477048071670978 1103 18-19 -0.7797831603815482 1103 20-21 -0.8468322015615328 1103 22-23 -0.8820210960006563 1103 24-25 -0.8778018991466325 1103 26-27 -0.9693335378170715 1103 28-29 -1.0366017566241368 1103 30-31 -1.0343685915789251 1103 32-33 -1.058701339993675 1103 34-35 -1.0830260707834398 1103 36-37 -1.04279042041847 1103 38-39 -1.048050128631239 1103 40-41 -1.134225747525825 1103 42-43 -1.197306649250045 1103 44-45 -1.2496991475200119 1103 46-47 -1.1039311333591186 1103 48-49 -1.1287529751180827 1103 50-51 -1.2663915884973633 1103 52-53 -1.1055915378607537 1103 54-55 -0.8544166951098049 1103 56-57 -0.844487085573995 1103 58-59 -0.8208126685989825 1103 60-61 -0.9170714141240524 1103 62-63 -0.8425613975191624 1103 64-65 -0.7725475809275295 1103 66-67 -0.9170251440027002 1103 68-69 -0.9319806383123108 1103 70-71 -0.8941987149585628 1103 72-73 -0.8940479720553753 1103 74-75 -0.8382094049887812 1103 76-77 -0.9212410565400546 1103 78-79 -0.9112993073886138 1103 80-81 -0.9918963061073818 1103 82-83 -1.023447135998989 1103 84-85 -1.1208723641693403 1103 86-87 -1.1422675916422556 1103 88-89 -1.158576919287576 1103 90-91 -1.3462295526507901 1103 92-93 -1.3714440197519444 1103 94-95 -1.4109844075889377 1103 96-97 -1.5367047812713217 1103 98-99 -1.55955119703545 1103 100 -1.5227698880436087 1104 1 -0.5312900085078063 1104 2 -0.48391505297690784 1104 3 -0.5342822017524789 1104 4 -0.38838726059275785 1104 5 -0.3507214787152009 1104 6 -0.3910527767438339 1104 7 -0.3917519540919372 1104 8 -0.43423668958303807 1104 9 -0.4912705238579207 1104 10-11 -0.5172942775916596 1104 12-13 -0.4946316390903789 1104 14-15 -0.6848528323455554 1104 16-17 -0.832962141147604 1104 18-19 -0.8502046204026286 1104 20-21 -0.7868047551352575 1104 22-23 -0.7442105934407621 1104 24-25 -0.7498991746772816 1104 26-27 -0.771683075948637 1104 28-29 -0.8147971533284277 1104 30-31 -0.8921887440875693 1104 32-33 -0.938814368292924 1104 34-35 -1.0633552853401227 1104 36-37 -1.0335355546684397 1104 38-39 -1.0047277465037112 1104 40-41 -0.9645363004216208 1104 42-43 -0.9431423398700716 1104 44-45 -0.9787052962003386 1104 46-47 -1.1183439738271517 1104 48-49 -1.0814116790664556 1104 50-51 -1.0617597278048336 1104 52-53 -0.9279035023736455 1104 54-55 -0.7475366767672611 1104 56-57 -0.7973076068711364 1104 58-59 -0.8019455317797224 1104 60-61 -0.8311812990876462 1104 62-63 -0.82171706889131 1104 64-65 -0.8087080411837775 1104 66-67 -0.8734137836700384 1104 68-69 -0.9438950979779577 1104 70-71 -0.9320962960521371 1104 72-73 -0.8278865978672769 1104 74-75 -0.9536650668918369 1104 76-77 -0.8874461067160802 1104 78-79 -0.9081344343080815 1104 80-81 -1.0009392746481574 1104 82-83 -1.0404517065389918 1104 84-85 -1.0307296343138965 1104 86-87 -0.9995293639478326 1104 88-89 -1.1353266886181004 1104 90-91 -1.0478910477781618 1104 92-93 -1.0999501328028565 1104 94-95 -1.1629468826777902 1104 96-97 -1.2671325015822035 1104 98-99 -1.2224561390492177 1104 100 -1.1812592918312994 1105 1 -0.5985917478517884 1105 2 -0.5876913309224712 1105 3 -0.6390886019964555 1105 4 -0.45748439742990854 1105 5 -0.3775238215424537 1105 6 -0.44546034792696076 1105 7 -0.4504812164614691 1105 8 -0.48675517488829456 1105 9 -0.6674522982214413 1105 10-11 -0.6999955343944464 1105 12-13 -0.6568857996236446 1105 14-15 -0.9019963915012283 1105 16-17 -0.8661662679522237 1105 18-19 -0.8626523013400345 1105 20-21 -0.8695314352148458 1105 22-23 -0.8870584045643284 1105 24-25 -0.8923511262997934 1105 26-27 -0.8556787315562744 1105 28-29 -0.8297407676868289 1105 30-31 -0.9015049301354807 1105 32-33 -0.9743393942377168 1105 34-35 -0.9544589197868163 1105 36-37 -0.9807099080774719 1105 38-39 -1.035906161092548 1105 40-41 -1.0587844839590659 1105 42-43 -1.0764218898124156 1105 44-45 -1.0432098001963226 1105 46-47 -1.1306505495451162 1105 48-49 -1.1810569046114736 1105 50-51 -1.1290046937465164 1105 52-53 -1.0525198422005886 1105 54-55 -1.0023729280407707 1105 56-57 -0.8961833135322976 1105 58-59 -0.8883979868366936 1105 60-61 -0.8317347375024937 1105 62-63 -0.872396196174499 1105 64-65 -0.8392477652225026 1105 66-67 -0.8730667828354512 1105 68-69 -0.8321504398724642 1105 70-71 -0.8622220016907249 1105 72-73 -0.8153191426393249 1105 74-75 -0.8954034999655676 1105 76-77 -0.8387910350760635 1105 78-79 -0.8770805183094836 1105 80-81 -0.9175166806828621 1105 82-83 -1.0060246916926623 1105 84-85 -0.9966952374036744 1105 86-87 -1.0530813051482575 1105 88-89 -1.1060929164443607 1105 90-91 -1.0851425905176555 1105 92-93 -1.0980973279104624 1105 94-95 -1.1470795654730104 1105 96-97 -1.2570262224275481 1105 98-99 -1.1847431488039355 1105 100 -1.2352683039135606 1106 1 -0.5440571022626308 1106 2 -0.5904407898253581 1106 3 -0.6669506544539239 1106 4 -0.5085188292757223 1106 5 -0.44988759576975923 1106 6 -0.5141530690883798 1106 7 -0.4669451638866562 1106 8 -0.4743441898926193 1106 9 -0.6194583422715354 1106 10-11 -0.6303597166185426 1106 12-13 -0.6900606120221937 1106 14-15 -0.8599131532972066 1106 16-17 -0.832237553274858 1106 18-19 -0.9466556809221487 1106 20-21 -0.8587113390791714 1106 22-23 -0.9127488052566264 1106 24-25 -0.862415819877711 1106 26-27 -0.9262833702025048 1106 28-29 -1.004132932776585 1106 30-31 -1.0411679930255744 1106 32-33 -1.0423456056266218 1106 34-35 -1.016330309765813 1106 36-37 -1.0775717586953704 1106 38-39 -1.1000415327392474 1106 40-41 -1.1678214533665354 1106 42-43 -1.0539871004983112 1106 44-45 -1.1087159720404856 1106 46-47 -1.1042970885048788 1106 48-49 -1.095316865386323 1106 50-51 -1.1611306878830803 1106 52-53 -1.0965877773575414 1106 54-55 -0.9225893604290789 1106 56-57 -0.938980969804426 1106 58-59 -0.9390091083989205 1106 60-61 -0.990776926411904 1106 62-63 -0.9269402386829881 1106 64-65 -0.9821335729710015 1106 66-67 -1.057628623906119 1106 68-69 -1.0548180044468012 1106 70-71 -1.008657126247364 1106 72-73 -0.9667532567875554 1106 74-75 -0.9522023895089617 1106 76-77 -0.9868005647930858 1106 78-79 -1.0771236553645238 1106 80-81 -0.980147844123767 1106 82-83 -0.9329835826774229 1106 84-85 -1.0149023057996907 1106 86-87 -1.054484164830047 1106 88-89 -1.0434532286912308 1106 90-91 -1.1367725994136073 1106 92-93 -1.2168316255220653 1106 94-95 -1.2933815644837487 1106 96-97 -1.199008421807438 1106 98-99 -1.159124185883261 1106 100 -1.3751359856048886 1107 1 -0.6254597973775162 1107 2 -0.6171246067278169 1107 3 -0.5314193789698969 1107 4 -0.4635539337690986 1107 5 -0.4020357747196428 1107 6 -0.4662547995649504 1107 7 -0.49131783732634204 1107 8 -0.4423954095279399 1107 9 -0.6053417041658093 1107 10-11 -0.6367943299382404 1107 12-13 -0.6527650350920808 1107 14-15 -0.7954989750044845 1107 16-17 -0.776480601348176 1107 18-19 -0.861924263785518 1107 20-21 -0.8574093951579442 1107 22-23 -0.8557464136864041 1107 24-25 -0.836389696835397 1107 26-27 -0.9165206790226179 1107 28-29 -0.9608225981741896 1107 30-31 -0.9298726517110723 1107 32-33 -0.9952371814264325 1107 34-35 -1.008510311476428 1107 36-37 -1.0628103689676678 1107 38-39 -1.0573417600240234 1107 40-41 -1.008701031082147 1107 42-43 -0.98575503570396 1107 44-45 -0.9744519344301779 1107 46-47 -0.8131199981389372 1107 48-49 -0.7418742787136239 1107 50-51 -0.8093476728063678 1107 52-53 -0.7368539746104119 1107 54-55 -0.7352311823059807 1107 56-57 -0.631901694356948 1107 58-59 -0.7494736445666561 1107 60-61 -0.7080918521847721 1107 62-63 -0.7140433219479903 1107 64-65 -0.6737071147758584 1107 66-67 -0.7246583862448261 1107 68-69 -0.6643170004743126 1107 70-71 -0.6958489975934157 1107 72-73 -0.6745317740234498 1107 74-75 -0.675749524191346 1107 76-77 -0.6914452311693786 1107 78-79 -0.663557758665636 1107 80-81 -0.742995319247008 1107 82-83 -0.7350592767342015 1107 84-85 -0.6395972940762924 1107 86-87 -0.6626030422773503 1107 88-89 -0.7224750122384549 1107 90-91 -0.847766505576498 1107 92-93 -0.8922104935634287 1107 94-95 -0.9001636296926279 1107 96-97 -0.8748899522176607 1107 98-99 -0.8849075150944508 1107 100 -0.9720771308849834 1108 1 -0.6639839665015472 1108 2 -0.6516723329921241 1108 3 -0.7097453162939189 1108 4 -0.49824286668501827 1108 5 -0.5014493689637121 1108 6 -0.4929604678329653 1108 7 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1.1738596094975051 2311 64-65 1.173833396637356 2311 66-67 1.1457999586210406 2311 68-69 1.1246667890829016 2311 70-71 1.110218554270098 2311 72-73 1.1473082059114503 2311 74-75 1.139486575829963 2311 76-77 1.179013703855908 2311 78-79 1.2028794004886905 2311 80-81 1.1706740006506244 2311 82-83 1.1860623113705095 2311 84-85 1.157684157022782 2311 86-87 1.156088312687654 2311 88-89 1.2260889367820766 2311 90-91 1.3199599576031424 2311 92-93 1.3383030585885471 2311 94-95 1.3782884719128496 2311 96-97 1.3909082039942327 2311 98-99 1.4645739062011778 2311 100 1.6132489975144857 2312 1 0.6395179476757029 2312 2 0.66158512369935 2312 3 0.7343297129778357 2312 4 0.4157090541331243 2312 5 0.5477655448044345 2312 6 0.5866794166790896 2312 7 0.47256123179307963 2312 8 0.6336040587383422 2312 9 0.8031316175011156 2312 10-11 0.7817325893258271 2312 12-13 0.9009490859417681 2312 14-15 1.1187264635835135 2312 16-17 1.1095867379324957 2312 18-19 1.0975901800952172 2312 20-21 1.0998403550177969 2312 22-23 1.1526900967995601 2312 24-25 1.1307357895442323 2312 26-27 1.1966703447844793 2312 28-29 1.1826911516371723 2312 30-31 1.2051637156745656 2312 32-33 1.2483004594718778 2312 34-35 1.2578169809166013 2312 36-37 1.333930697702293 2312 38-39 1.2955868322347897 2312 40-41 1.3385406483946625 2312 42-43 1.4094611237108623 2312 44-45 1.4377491417198272 2312 46-47 1.510513292962294 2312 48-49 1.5454193876295008 2312 50-51 1.5679034521563935 2312 52-53 1.3956879300372904 2312 54-55 1.32887810642805 2312 56-57 1.2426216162766437 2312 58-59 1.2208370320472994 2312 60-61 1.2059822548799346 2312 62-63 1.208860844365283 2312 64-65 1.2102343430968432 2312 66-67 1.153689826173796 2312 68-69 1.182100493318785 2312 70-71 1.1721548381744071 2312 72-73 1.2231291657791203 2312 74-75 1.2426372018336238 2312 76-77 1.2377856896797255 2312 78-79 1.2543561604129252 2312 80-81 1.2077682920045767 2312 82-83 1.2591186044183509 2312 84-85 1.2876952496792846 2312 86-87 1.3367414837045821 2312 88-89 1.2253227102708522 2312 90-91 1.28589290079643 2312 92-93 1.400579685214435 2312 94-95 1.447639071310121 2312 96-97 1.5136986400771377 2312 98-99 1.1452520664135015 2312 100 1.3874731220073322 2313 1 0.5859498151116931 2313 2 0.6276058065513546 2313 3 0.6286506579606446 2313 4 0.4952635831428509 2313 5 0.39521212839994035 2313 6 0.5168098765811493 2313 7 0.5856456207558338 2313 8 0.6011107886124236 2313 9 0.7591093603505428 2313 10-11 0.7741017068356015 2313 12-13 0.8763802141638379 2313 14-15 1.0610650538294166 2313 16-17 1.0482578054027911 2313 18-19 1.0268843443419868 2313 20-21 1.0741177923422995 2313 22-23 1.1224281930588305 2313 24-25 1.0664488660617053 2313 26-27 1.0567038743164545 2313 28-29 1.084401397444715 2313 30-31 1.1001126645022978 2313 32-33 1.1700616681496925 2313 34-35 1.2343056013113411 2313 36-37 1.2554858347343867 2313 38-39 1.2909741500488323 2313 40-41 1.2627960480621354 2313 42-43 1.3464065177522357 2313 44-45 1.3240986486162143 2313 46-47 1.3732218812151018 2313 48-49 1.3234464181340613 2313 50-51 1.4152620743423938 2313 52-53 1.318874358271735 2313 54-55 1.128648975344987 2313 56-57 1.0746175190637146 2313 58-59 1.0672648754348657 2313 60-61 1.0227855331548596 2313 62-63 1.0560941029362638 2313 64-65 1.120886529075996 2313 66-67 1.1038234901771062 2313 68-69 1.110510048100089 2313 70-71 1.097416729300562 2313 72-73 1.0845808885512014 2313 74-75 1.0239790390703263 2313 76-77 0.8342720016078076 2313 78-79 1.0516142696904396 2313 80-81 1.1026539282961956 2313 82-83 1.1405757640708991 2313 84-85 1.1922218626415209 2313 86-87 1.2071740752169937 2313 88-89 1.2590214541117817 2313 90-91 1.3073952836890577 2313 92-93 1.389768858379714 2313 94-95 1.4631202289241507 2313 96-97 1.452744208956485 2313 98-99 1.479363004004167 2313 100 1.571083614303614 2314 1 0.611445210472386 2314 2 0.5835190340296421 2314 3 0.6115314814705606 2314 4 0.48211930811294224 2314 5 0.471135553764924 2314 6 0.49099373689700343 2314 7 0.5320565332282499 2314 8 0.5252786734618979 2314 9 0.6730998643327979 2314 10-11 0.6964957402159513 2314 12-13 0.773573432324639 2314 14-15 0.8938443478397531 2314 16-17 0.8839282981111225 2314 18-19 0.8794843255113562 2314 20-21 0.9105864103024359 2314 22-23 0.9263469541745621 2314 24-25 0.9343962662425582 2314 26-27 1.0216384959583493 2314 28-29 1.0153838886270279 2314 30-31 1.0011323186667695 2314 32-33 1.053824238970897 2314 34-35 1.0448046496919972 2314 36-37 1.067026868083559 2314 38-39 1.1108610439914273 2314 40-41 1.155478935971594 2314 42-43 1.2291094132061673 2314 44-45 1.2903112372592602 2314 46-47 1.2500394283054348 2314 48-49 1.1926546940737026 2314 50-51 1.2267598981207115 2314 52-53 1.1893206228066262 2314 54-55 1.029669577154806 2314 56-57 1.0031230089871812 2314 58-59 1.0028342024171337 2314 60-61 0.9919912565659246 2314 62-63 0.9998952520938005 2314 64-65 1.0388252414888726 2314 66-67 1.0253192535910287 2314 68-69 1.0497264921312421 2314 70-71 1.0336154548092793 2314 72-73 1.0351379725669858 2314 74-75 1.0846738098410285 2314 76-77 1.1026473737114557 2314 78-79 1.1030478308866307 2314 80-81 1.084653105373203 2314 82-83 1.0794397421148751 2314 84-85 1.161819489370739 2314 86-87 1.187293701685764 2314 88-89 1.1868552469002651 2314 90-91 1.2894916402678227 2314 92-93 1.3201832270064138 2314 94-95 1.3166535979623148 2314 96-97 1.3611504192738373 2314 98-99 1.4711377022402665 2314 100 1.535970688604511 2315 1 0.4489999667459408 2315 2 0.46137029881052527 2315 3 0.5231518203210221 2315 4 0.16310033542272606 2315 5 0.2574400463833655 2315 6 0.3412935722495192 2315 7 0.38049884035404347 2315 8 0.39136211589628545 2315 9 0.620392724205395 2315 10-11 0.475390070538495 2315 12-13 0.4884323776558901 2315 14-15 0.7142659189997929 2315 16-17 0.7321914552143909 2315 18-19 0.6092261533811651 2315 20-21 0.7363558441059226 2315 22-23 0.5487412699557765 2315 24-25 0.7418906553756202 2315 26-27 0.8313908593961727 2315 28-29 0.8412938846218054 2315 30-31 0.8878152194305997 2315 32-33 0.8640805477904223 2315 34-35 0.9035772921002376 2315 36-37 1.0062397540133574 2315 38-39 1.0243748750566013 2315 40-41 1.0221399118660415 2315 42-43 1.1005425928774883 2315 44-45 1.0843920872542583 2315 46-47 1.133708961166782 2315 48-49 1.1410209614584232 2315 50-51 0.9699106756065987 2315 52-53 1.037575902503697 2315 54-55 0.9344694837800702 2315 56-57 0.9244822371205501 2315 58-59 0.8803069432862003 2315 60-61 0.8837838907733158 2315 62-63 0.8655321563684808 2315 64-65 0.8778262466411988 2315 66-67 0.9014556002858001 2315 68-69 0.9045200454124469 2315 70-71 0.8345545814874171 2315 72-73 0.6136659074213071 2315 74-75 0.8434102661307676 2315 76-77 0.8558621707981402 2315 78-79 0.9170302850558798 2315 80-81 0.6090093489598054 2315 82-83 0.6811996992194729 2315 84-85 0.9042014896016006 2315 86-87 1.0447074606742852 2315 88-89 0.9967438415719982 2315 90-91 1.0918573590371814 2315 92-93 1.1538668595727621 2315 94-95 1.1125097379438422 2315 96-97 0.98963356750399 2315 98-99 0.46289984227666636 2315 100 1.0022657297458757 2316 1 0.38730861101616654 2316 2 0.33780933497033416 2316 3 0.3753360099191383 2316 4 0.26508944791932976 2316 5 0.16429802349625788 2316 6 0.22775638936700204 2316 7 0.18532986938841134 2316 8 0.21608232824011253 2316 9 0.36559650901290297 2316 10-11 0.295491082041778 2316 12-13 0.07219726839484508 2316 14-15 0.12054817714369648 2316 16-17 0.28522539504957223 2316 18-19 0.3218215301319418 2316 20-21 0.37636419726661785 2316 22-23 0.34399504558999183 2316 24-25 0.39008465236983625 2316 26-27 0.4361302066164754 2316 28-29 0.3690472458875149 2316 30-31 0.2747393313831239 2316 32-33 0.48190361084346023 2316 34-35 0.5013847652546986 2316 36-37 0.4714188432063935 2316 38-39 0.6277893682862086 2316 40-41 0.6738511513460494 2316 42-43 0.6071916768449128 2316 44-45 0.5731432338558591 2316 46-47 0.47269444077060285 2316 48-49 0.3806193709579091 2316 50-51 0.482974149521759 2316 52-53 0.5680882478229989 2316 54-55 0.3049350789983194 2316 56-57 0.14995026510971599 2316 58-59 0.1502023108013404 2316 60-61 0.29938273641517554 2316 62-63 0.44515027742237123 2316 64-65 0.3989242892626095 2316 66-67 0.3361743651157951 2316 68-69 0.3403912397909181 2316 70-71 0.09456928356801342 2316 72-73 0.012016261204777834 2316 74-75 0.2333724322114179 2316 76-77 0.3937796377845473 2316 78-79 0.456005346217772 2316 80-81 0.5977088783747071 2316 82-83 0.6063809819499717 2316 84-85 0.6954353148161054 2316 86-87 0.8011037255256852 2316 88-89 0.7289096018215488 2316 90-91 0.5605934709233402 2316 92-93 0.6115249689890625 2316 94-95 0.7492652909537973 2316 96-97 0.7162775268045429 2316 98-99 0.5466927267147668 2316 100 0.4294578085853473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 36.0 1 673.0 2 270.0 3 220.0 4 180.0 5 155.0 6 168.0 7 713.0 8 2251.0 9 4420.0 10 7245.0 11 10467.0 12 14957.0 13 19313.0 14 23581.0 15 28292.0 16 34790.0 17 43565.0 18 53305.0 19 66618.0 20 83565.0 21 103726.0 22 114175.0 23 113975.0 24 110894.0 25 114339.0 26 125346.0 27 143190.0 28 169709.0 29 203723.0 30 247075.0 31 306678.0 32 390270.0 33 512824.0 34 734250.0 35 1017150.0 36 1528815.0 37 2081674.0 38 1788804.0 39 543652.0 40 523.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60661587104362 20.52500915311949 12.952297256075418 22.916077719761464 2 15.494018992738182 19.632035499330474 41.64443454624576 23.229510961685584 3 19.306177231354614 26.35546421613934 28.488003790089362 25.850354762416682 4 12.334578788761322 16.26897444038042 39.58364688448594 31.81279988637232 5 12.997864673683027 38.45235800396461 34.53388854361735 14.015888778735015 6 29.90477940940273 38.01842251715059 17.965729823876313 14.11106824957037 7 27.35660516521328 32.20411975654926 22.628705960963817 17.81056911727364 8 26.289535720493962 35.336753033126854 19.654704855600656 18.71900639077852 9 26.37422486814135 15.567815885637753 21.59001798396218 36.46794126225871 10-11 25.842723930975865 26.332056137885907 26.926775239684197 20.898444691454028 12-13 25.898650314996015 23.966346737953238 27.73984715182511 22.395155795225637 14-15 23.197018904706518 25.137761495994624 25.823455485179597 25.841764114119258 16-17 21.630531204396114 27.990308340152463 26.97895472501693 23.400205730434493 18-19 21.020498135439475 27.896030453984384 29.516694202998984 21.56677720757716 20-21 22.678885694236005 26.948128317009513 28.991360102702156 21.38162588605232 22-23 22.90238734602722 26.7780267505479 28.586343533273613 21.733242370151267 24-25 22.2474584459461 26.679301326872036 29.521895087329487 21.551345139852376 26-27 22.207876601892483 26.99417838882819 28.484595365003607 22.313349644275725 28-29 21.378412360877142 27.42458037717595 29.061302645438474 22.13570461650843 30-31 22.33641149352099 27.036909021888373 29.01370319345294 21.612976291137702 32-33 22.532551186438155 27.221262431258076 28.179339630720428 22.066846751583345 34-35 22.54338117184281 27.132248759628723 28.91721147995772 21.407158588570745 36-37 22.11417188907961 26.787039811874454 28.610043611032392 22.488744688013547 38-39 22.338350258741137 26.984232402686835 29.024463835091495 21.65295350348053 40-41 22.17854419957478 26.08755165804925 29.481599464873504 22.25230467750246 42-43 22.059673880446226 26.269096178416078 28.9135922028679 22.75763773826979 44-45 22.260881514410634 27.107467008356384 28.69123441306775 21.94041706416523 46-47 22.186142797894494 26.956883717208175 28.10093644137567 22.756037043521665 48-49 22.840919088433935 26.90806834743855 28.062403090130196 22.188609473997317 50-51 22.546597828502676 26.74765739632095 28.05802605750244 22.647718717673932 52-53 22.057217442387124 27.322299292311314 27.74521345404492 22.87526981125664 54-55 21.936619397064472 27.245442644451323 27.830022512378456 22.987915446105745 56-57 22.84534395587331 26.829257279939107 27.413913744866143 22.91148501932144 58-59 21.986222048390786 27.244840739567312 28.122902590641836 22.646034621400066 60-61 22.088438571913933 27.772895034464142 27.527406090449176 22.61126030317275 62-63 22.377299734034928 27.092971603955927 27.240940971988216 23.28878769002093 64-65 21.768306424370422 27.169487137789712 27.617139324686136 23.445067113153737 66-67 22.212927017937883 26.862297902034694 27.52035368135937 23.404421398668056 68-69 21.826651781551895 26.908443184602127 27.782390520696154 23.482514513149823 70-71 21.83373510419742 26.524143445092758 27.864622536948165 23.77749891376166 72-73 22.351770383608873 26.601728208120857 28.0280836612718 23.018417746998466 74-75 22.533374248028412 26.823180424357002 27.654365278522537 22.98908004909205 76-77 22.225475394868376 26.75922059195921 27.343348916449173 23.671955096723245 78-79 22.15022005084784 26.77559746769792 27.36807141409081 23.706111067363427 80-81 21.76537119097161 27.163757764595235 27.857652395693215 23.213218648739932 82-83 22.580870206584404 26.336404905840883 28.771249061164028 22.311475826410685 84-85 22.693974620994194 26.65752870933892 27.648519612287398 22.999977057379496 86-87 21.56859396210996 27.290888808947518 27.994738451328814 23.145778777613707 88-89 22.521777219965884 26.17217597955837 27.886467119018025 23.41957968145772 90-91 22.265888136017605 26.253903008522045 27.914810279694862 23.565398575765485 92-93 22.533863375178605 26.387107244209794 27.873797456742977 23.205231923868624 94-95 21.505826448059963 26.399647213369587 28.476513276622185 23.61801306194826 96-97 21.793791056224162 26.312370321207794 28.06352852910157 23.83031009346647 98-99 21.3109299330874 26.63580230680751 27.911038817388217 24.14222894271687 100 22.065098672730596 26.19310284608763 27.100262501104268 24.64153598007751 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1211.0 1 877.0 2 840.5 3 1168.0 4 999.5 5 902.0 6 1344.5 7 2501.5 8 4184.5 9 5078.5 10 4814.0 11 4685.5 12 5258.5 13 6316.5 14 7579.5 15 8968.5 16 10372.0 17 11621.0 18 12771.5 19 13919.0 20 15140.0 21 16474.5 22 18406.0 23 21043.5 24 24119.5 25 28273.5 26 34612.5 27 42221.5 28 53059.5 29 72536.0 30 99584.5 31 126078.0 32 161953.0 33 200331.0 34 229595.5 35 260704.5 36 288732.5 37 331666.0 38 353056.0 39 339137.5 40 320658.0 41 299664.0 42 294776.0 43 308406.5 44 334481.5 45 383734.5 46 431205.0 47 464318.0 48 487897.5 49 484142.5 50 466244.0 51 476834.5 52 478345.0 53 448824.5 54 418157.5 55 358515.5 56 308295.0 57 253953.5 58 198592.5 59 184312.0 60 151883.0 61 104641.0 62 77108.0 63 64900.5 64 48073.0 65 24394.0 66 13883.0 67 9351.5 68 6919.0 69 5425.0 70 4145.0 71 2996.0 72 2143.0 73 1686.5 74 1316.5 75 968.0 76 724.5 77 520.5 78 377.0 79 283.0 80 205.0 81 148.5 82 121.5 83 105.0 84 79.0 85 56.5 86 44.5 87 30.5 88 26.5 89 26.5 90 21.5 91 16.5 92 12.0 93 12.5 94 15.5 95 10.0 96 5.5 97 5.0 98 2.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05646975090027747 2 0.13127262791682828 3 0.016434670416923206 4 0.015960056492085675 5 0.01878912773033293 6 0.051807367050402876 7 0.05727938641911797 8 0.0489224588798218 9 0.0403794082327462 10-11 0.03910911802215163 12-13 0.040286346678856486 14-15 0.018686760021054245 16-17 0.017732879093684695 18-19 0.017602592918239096 20-21 0.021190115820687506 22-23 0.025759438116672386 24-25 0.02457755638227304 26-27 0.019287007043642893 28-29 0.018328473038578853 30-31 0.016439323494617693 32-33 0.023228163850872213 34-35 0.023474776968679948 36-37 0.023614369299514517 38-39 0.021492565870829074 40-41 0.025084741850971974 42-43 0.03231562458820262 44-45 0.04138447301475509 46-47 0.047224085521334544 48-49 0.03983965122018587 50-51 0.02781144537994054 52-53 0.03974658966629616 54-55 0.03657319067865697 56-57 0.03852748331034092 58-59 0.043245704092549346 60-61 0.023507348512541347 62-63 0.02616890895378712 64-65 0.02558262116428193 66-67 0.032362155365147485 68-69 0.0390532810898178 70-71 0.04075165444830505 72-73 0.04411117654372367 74-75 0.026983197550322108 76-77 0.02063639957504372 78-79 0.020008234086288164 80-81 0.024777638723135924 82-83 0.020650358808127177 84-85 0.012847147514474794 86-87 0.014806093223853239 88-89 0.018705372331832188 90-91 0.021041217334463968 92-93 0.019984968697815735 94-95 0.023623675454903487 96-97 0.024996333374776747 98-99 0.027736996136828774 100 0.029267858698314546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0745576E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.66394164942517 #Duplication Level Percentage of deduplicated Percentage of total 1 75.39513397982569 48.75346544322041 2 14.919814887114288 19.29548078561167 3 4.981252593359016 9.663222811140455 4 2.0449353557953747 5.289343220959944 5 1.0534668428742702 3.4060659228612984 6 0.5582660985795475 2.1659811854040054 7 0.30931404861782213 1.4001025913819218 8 0.18656933984625698 0.9651447124312108 9 0.12254287340158654 0.7131694693673778 >10 0.39526893628515425 4.071522112208005 >50 0.01962934323114272 0.8846690995446539 >100 0.012398424543767826 1.5016855870810963 >500 8.774736106488487E-4 0.36988046515336126 >1k 4.5672669417124606E-4 0.466231461398227 >5k 1.8269055328188236E-5 0.08374583966492742 >10k+ 5.48071659845647E-5 0.9702892925714697 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36318 0.3379809514166574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28185 0.26229398963815437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17631 0.1640768256629519 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.306155388971239E-6 0.0 0.0 0.0 0.0 3 9.306155388971239E-6 0.0 0.0 0.0 0.0 4 9.306155388971239E-6 0.0 0.0 0.0 0.0 5 9.306155388971239E-6 0.0 0.0 9.306155388971239E-6 0.0 6 9.306155388971239E-6 0.0 0.0 9.306155388971239E-6 0.0 7 9.306155388971239E-6 0.0 0.0 9.306155388971239E-6 0.0 8 9.306155388971239E-6 0.0 0.0 1.8612310777942478E-5 0.0 9 9.306155388971239E-6 0.0 0.0 3.7224621555884955E-5 9.306155388971239E-6 10-11 9.306155388971239E-6 0.0 0.0 4.653077694485619E-5 9.306155388971239E-6 12-13 9.306155388971239E-6 0.0 0.0 5.1183854639341814E-5 9.306155388971239E-6 14-15 9.306155388971239E-6 0.0 0.0 6.0490010028313044E-5 9.306155388971239E-6 16-17 9.306155388971239E-6 0.0 0.0 7.444924311176991E-5 9.306155388971239E-6 18-19 1.3959233083456859E-5 0.0 0.0 8.840847619522676E-5 9.306155388971239E-6 20-21 1.8612310777942478E-5 0.0 0.0 1.3959233083456858E-4 1.8612310777942478E-5 22-23 3.7224621555884955E-5 0.0 0.0 3.1640928322502213E-4 1.8612310777942478E-5 24-25 6.514308772279867E-5 0.0 0.0 7.956762857570408E-4 1.8612310777942478E-5 26-27 7.444924311176991E-5 0.0 0.0 0.001670454892320337 1.8612310777942478E-5 28-29 7.444924311176991E-5 0.0 0.0 0.005062548531600354 1.8612310777942478E-5 30-31 7.444924311176991E-5 0.0 0.0 0.01831916688318988 1.8612310777942478E-5 32-33 7.444924311176991E-5 0.0 0.0 0.0491504596868516 1.8612310777942478E-5 34-35 7.444924311176991E-5 0.0 0.0 0.09595111513798796 1.8612310777942478E-5 36-37 7.444924311176991E-5 0.0 0.0 0.17567229527761008 1.8612310777942478E-5 38-39 7.444924311176991E-5 0.0 0.0 0.2913478067625226 1.8612310777942478E-5 40-41 7.910232080625553E-5 0.0 0.0 0.41232782681914865 1.8612310777942478E-5 42-43 8.840847619522676E-5 0.0 0.0 0.52964122165252 1.8612310777942478E-5 44-45 9.306155388971238E-5 0.0 0.0 0.666199745830284 1.8612310777942478E-5 46-47 9.771463158419799E-5 0.0 0.0 0.8290574651372806 1.8612310777942478E-5 48-49 1.0702078697316924E-4 0.0 0.0 1.0085406310466745 1.8612310777942478E-5 50-51 1.1632694236214048E-4 0.0 0.0 1.1927280585051931 1.8612310777942478E-5 52-53 1.2098002005662609E-4 0.0 0.0 1.3679676175572162 2.3265388472428096E-5 54-55 1.3959233083456858E-4 0.0 0.0 1.5565568565147183 2.7918466166913715E-5 56-57 1.4889848622353982E-4 0.0 0.0 1.778220171724624 2.7918466166913715E-5 58-59 1.4889848622353982E-4 0.0 0.0 2.0338276887158027 2.7918466166913715E-5 60-61 1.4889848622353982E-4 0.0 0.0 2.2905891689752136 2.7918466166913715E-5 62-63 1.4889848622353982E-4 0.0 0.0 2.5498121273350076 2.7918466166913715E-5 64-65 1.4889848622353982E-4 0.0 0.0 2.8099191704567534 2.7918466166913715E-5 66-67 1.4889848622353982E-4 0.0 0.0 3.102835064402318 2.7918466166913715E-5 68-69 1.4889848622353982E-4 0.0 0.0 3.424455794645164 2.7918466166913715E-5 70-71 1.4889848622353982E-4 0.0 0.0 3.77488372889457 2.7918466166913715E-5 72-73 1.4889848622353982E-4 0.0 0.0 4.126246931760568 2.7918466166913715E-5 74-75 1.4889848622353982E-4 0.0 0.0 4.464051066224835 2.7918466166913715E-5 76-77 1.4889848622353982E-4 0.0 0.0 4.8402663570570805 2.7918466166913715E-5 78-79 1.4889848622353982E-4 0.0 0.0 5.2407986319207085 2.7918466166913715E-5 80-81 1.5820464161251104E-4 0.0 0.0 5.668914351357246 2.7918466166913715E-5 82-83 1.5820464161251104E-4 0.0 0.0 6.109393298228033 2.7918466166913715E-5 84-85 1.5820464161251104E-4 0.0 0.0 6.529896582556393 2.7918466166913715E-5 86-87 1.5820464161251104E-4 0.0 0.0 6.967020660409457 2.7918466166913715E-5 88 1.5820464161251104E-4 0.0 0.0 7.324484048132924 2.7918466166913715E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22045 0.0 43.37966 1 GTATCAA 38370 0.0 39.33505 1 GTACTAG 10740 0.0 37.772484 1 GCATCGT 10585 0.0 34.637638 8 CAACGCA 42955 0.0 33.498276 5 ATCAACG 43170 0.0 33.27731 3 GGGCACC 10765 0.0 32.917667 94 TAGGCAT 13180 0.0 32.13529 5 TATCAAC 47615 0.0 31.469929 2 CTGTGCG 13205 0.0 31.004322 9 GTGGTAT 7505 0.0 30.287888 1 AACGCAG 49035 0.0 30.131386 6 CTAGGCA 14725 0.0 29.593288 4 TACCTGG 5750 0.0 29.46464 2 TAGTACT 14315 0.0 28.89749 4 TCAACGC 51020 0.0 28.479172 4 ACTGTGC 15260 0.0 28.030535 8 ACGCAGA 52115 0.0 27.565731 7 CGCAGAG 52440 0.0 27.294125 8 CATCGTC 13765 0.0 26.942835 9 >>END_MODULE