FastQCFastQC Report
Mon 6 Jun 2016
ERR1211225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10745576
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331900.3088712973599554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276510.2573245026604437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172840.16084758974297889No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219400.045.451941
GTATCAA373650.037.975421
GGGCACC109450.034.18936594
CAACGCA435150.031.1076765
ATCAACG437950.030.9181423
CTGTGCG138550.030.6230569
TAGGCAT137450.030.0132545
TATCAAC476850.029.5859262
TACCTGG57100.028.888652
GTGGTAT74000.028.7815931
TAGTACT150650.028.670884
GCATCGT128900.028.6142278
ACTGTGC160350.028.1591648
AACGCAG497350.028.1337646
CTAACGC46450.028.0277823
CTAGGCA154950.027.450594
TAACGCC48000.027.1227174
CGCCTAC48200.026.8071697
TCTAACG49100.026.799882
TCAACGC513350.026.7431554
Produced by FastQC (version 0.11.5)