FastQCFastQC Report
Mon 6 Jun 2016
ERR1211224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9787466
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300700.3072296751784374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237280.24243251521895454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147030.15022274406879166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161650.048.502821
GTATCAA283800.040.2959631
CAACGCA331900.032.7880865
ATCAACG336000.032.5700383
GTACTAG63050.032.3945771
TATCAAC355200.031.850962
AACGCAG371450.029.7655376
GTGGTAT57400.029.598041
TCAACGC378250.029.1928424
GCATCGT64200.028.0423188
TACCTGG66300.027.3990042
ACGCAGA403850.026.9236897
GTACATG378300.026.634711
CGCAGAG410450.026.4202848
TAGGCAT84250.026.1682875
TACATGG379500.025.9424462
CTGTGCG83000.025.937939
TAGTACT85100.025.1335724
ACATGGG380650.024.9459533
CTAACGC32000.024.3855553
Produced by FastQC (version 0.11.5)